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1.
Artigo em Inglês | MEDLINE | ID: mdl-35038856

RESUMO

Background: Sugammadex is a specific antagonist of aminosteroidal neuromuscular blocking agents with 1:1 binding to guest molecules. Sugammadex can also bind to other drugs having a steroid component in its chemical structure. In this in vivo experiment, we investigated the differences in the recovery of rocuronium-induced neuromuscular blockade using sugammadex pre-exposed with two different concentrations of hydrocortisone. Methods: The sciatic nerves and tibialis anterior muscles of 30 adult Sprague-Dawley rats were prepared for the experiment. The sciatic nerves were stimulated using a train-of-four (TOF) pattern with indirect supramaximal stimulation at 20 s intervals. After 15 min of stabilization, a 250 µg loading dose and 125 µg booster doses of rocuronium were serially administered until > 95% depression of the first twitch tension of TOF stimulation (T1) was confirmed. The study drugs were prepared by mixing sugamadex with the same volume of three different stock solutions (0.9% normal saline, 10 mg/ml hydrocortisone, and 100 mg/ml hydrocortisone). The recovery of rats from neuromuscular blockade was monitored by assessing T1 and the TOF ratio (TOFR) simultaneously until T1 was recovered to > 95% and TOFR to > 0.9. Results: In the group injected with sugammadex premixed with a high concentration of hydrocortisone, statistically significant intergroup differences were observed in the recovery progression of T1 and TOFR (P < 0.050). Conclusions: When sugammadex was pre-exposed to a high dose of hydrocortisone only, recovery from neuromuscular blockade was delayed. Delayed recovery from neuromuscular blockade is not always plausible when sugammadex is pre-exposed to steroidal drugs.

2.
Mol Neurodegener ; 17(1): 6, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-35012591

RESUMO

BACKGROUND: Viral tracers are important tools for mapping brain connectomes. The feature of predominant anterograde transneuronal transmission offers herpes simplex virus-1 (HSV-1) strain H129 (HSV1-H129) as a promising candidate to be developed as anterograde viral tracers. In our earlier studies, we developed H129-derived anterograde polysynaptic tracers and TK deficient (H129-dTK) monosynaptic tracers. However, their broad application is limited by some intrinsic drawbacks of the H129-dTK tracers, such as low labeling intensity due to TK deficiency and potential retrograde labeling caused by axon terminal invasion. The glycoprotein K (gK) of HSV-1 plays important roles in virus entry, egress, and virus-induced cell fusion. Its deficiency severely disables virus egress and spread, while only slightly limits viral genome replication and expression of viral proteins. Therefore, we created a novel H129-derived anterograde monosynaptic tracer (H129-dgK) by targeting gK, which overcomes the limitations of H129-dTK. METHODS: Using our established platform and pipeline for developing viral tracers, we generated a novel tracer by deleting the gK gene from the H129-G4. The gK-deleted virus (H129-dgK-G4) was reconstituted and propagated in the Vero cell expressing wildtype H129 gK (gKwt) or the mutant gK (gKmut, A40V, C82S, M223I, L224V, V309M), respectively. Then the obtained viral tracers of gKmut pseudotyped and gKwt coated H129-dgK-G4 were tested in vitro and in vivo to characterize their tracing properties. RESULTS: H129-dgK-G4 expresses high levels of fluorescent proteins, eliminating the requirement of immunostaining for imaging detection. Compared to the TK deficient monosynaptic tracer H129-dTK-G4, H129-dgK-G4 labeled neurons with 1.76-fold stronger fluorescence intensity, and visualized 2.00-fold more postsynaptic neurons in the downstream brain regions. gKmut pseudotyping leads to a 77% decrease in retrograde labeling by reducing axon terminal invasion, and thus dramatically improves the anterograde-specific tracing of H129-dgK-G4. In addition, assisted by the AAV helper trans-complementarily expressing gKwt, H129-dgK-G4 allows for mapping monosynaptic connections and quantifying the circuit connectivity difference in the Alzheimer's disease and control mouse brains. CONCLUSIONS: gKmut pseudotyped H129-dgK-G4, a novel anterograde monosynaptic tracer, overcomes the limitations of H129-dTK tracers, and demonstrates desirable features of strong labeling intensity, high tracing efficiency, and improved anterograde specificity.

3.
J Virol ; : JVI0182721, 2022 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-35020472

RESUMO

Human cytomegalovirus (HCMV) has a large (∼235-kb) genome with over 200 predicted open reading frames and exploits numerous cellular factors to facilitate its replication. A key feature of HCMV-infected cells is the emergence of a distinctive membranous cytoplasmic compartment termed the virion assembly compartment (vAC). Here we report that host protein WD repeat domain 11 (WDR11) plays a key role in vAC formation and virion morphogenesis. We found that WDR11 was up-regulated at both mRNA and protein levels during HCMV infection. At the late stage of HCMV replication, WDR11 relocated to the vAC and co-localized with markers of the trans-Golgi network (TGN) and vAC. Depletion of WDR11 hindered HCMV-induced membrane reorganization of the Golgi and TGN, altered vAC formation, and impaired HCMV secondary envelopment and virion morphogenesis. Further, motifs critical for the localization of WDR11 in TGN were identified by alanine-scanning mutagenesis. Mutation of these motifs led to WDR11 mislocation outside of the TGN and loss of vAC formation. Taken together, these data indicate that host protein WDR11 is required for efficient viral replication at the stage of virion assembly, possibly by facilitating the remodeling of the endomembrane system for vAC formation and virion morphogenesis. Importance During the late phase of human cytomegalovirus (HCMV) infection, the endomembrane system is dramatically reorganized, resulting in the formation of a unique structure termed the virion assembly compartment (vAC), which is critical for the assembly of infectious virions. The mechanism of HCMV-induced vAC formation is still not fully understood. In this report, we identified a host factor, WDR11, that plays an important role in vAC formation. Our findings argue that WDR11 contributes to the relocation of the Golgi and trans-Golgi network to the vAC, a membrane reorganization process that appears to be required for efficient virion maturation. The present work provides new insights into the vAC formation and HCMV virion morphogenesis and a potential novel target for anti-viral treatment.

4.
Adv Healthc Mater ; : e2101375, 2022 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-34981675

RESUMO

Interleukin-12 (IL12) is a pleiotropic cytokine with promising prospects for cancer immunotherapy. Though IL12 gene-based therapy can overcome the fatal hurdle of severe systemic toxicity, targeted delivery and tumor-located expression of IL12 gene remain the challenging issues yet to be solved. Photo-immunotherapy emerging as a novel and precise therapeutic strategy, which elaborately combines immune-activating agents with light-triggered photosensitizers for potentiated anticancer efficacy. Herein, an engineered stem cell-based biotherapy platform (MB/IL12-MSCs) incorporating immune gene plasmid IL12 (pIL12) and photosensitizer methylene blue (MB) is developed to realize tumor-homing delivery of therapeutic agents and photo-immunotherapy efficacy enhancement. The biotherapy platform retained tumor-tropic migration and penetration functions, which improved the intratumoral distribution of therapeutic agents, thereby promoting photodynamic effects and reinforcing immune responses. Importantly, MB/IL12-MSCs restricted the expression and distribution of IL12 at tumor site, which minimized potential toxicity while eliciting sufficient anticancer immunity. In noteworthy, activation of immunity induced by MB/IL12-MSCs established long-term systemic immunologic memory to prevent tumor relapse. The MB/IL12-MSCs outperform their monotherapy counterparts in breast tumor models, and the growth of tumor significantly arrested as well as re-challenging abscopal tumor growth slowdown. Collectively, this work reveals that MSCs-based strategy may advance more efficient, durable, and safer cancer photo-immunotherapy.

5.
Artigo em Inglês | MEDLINE | ID: mdl-35019237

RESUMO

INTRODUCTION: Topical steroids are a mainstay in the treatment of chronic rhinosinusitis (CRS). Multiple delivery methods of topical steroids following sinus surgery have been investigated. The purpose of this trial is to evaluate the effect of triamcinolone impregnated absorbable nasal packing on healing following endoscopic sinus surgery. METHODS: This is a prospective, randomized, double-blind, placebo-controlled trial. A total of 22 subjects (14 without polyps and 8 with polyps) were enrolled and were randomized to receive triamcinolone impregnated packing in one sinus cavity and normal saline soaked packing in the contralateral sinus cavity. Endoscopic evaluation was completed at the first two post-operative visits, and Lund-Kennedy (LK) endoscopic scores and perioperative sinus endoscopy (POSE) scores were calculated at each visit. RESULTS: The results of this study found no significant difference in the appearance of the sinuses at either post-operative visit. Mean LK scores for the triamcinolone and saline groups at the first and second post-op visits were 2.09 ± 1.23 v. 2.18 ± 1.01 (p=0.79) and 1.79 ± 1.08 v. 1.68 ± 1.16 (p = 0.77) respectively. POSE scores were 2.59 ± 1.71 v. 2.68 ± 1.62 (p = 0.86) and 1.74 ± 1.15 v. 1.95 ± 1.22 (p = 0.59). CONCLUSION: The results of this study demonstrated no significant difference in healing, crusting, polyps, edema, or secretions between the triamcinolone treated and non-triamcinolone treated sinuses. There were no adverse effects from use of triamcinolone impregnated absorbable packing. Further studies will be necessary to determine the impact of triamcinolone impregnated absorbable packing following endoscopic sinus surgery. This article is protected by copyright. All rights reserved.

6.
Opt Lett ; 47(2): 210-213, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35030569

RESUMO

The plasmonic hotspot of metal nanostructures has small dimension far beyond the optical diffraction limit. When trying to locate the hotspot using fluorescent probes, the localization is significantly distorted due to the coupling of emission and surface plasmon. A label-free technique can solve the problem, which uses hotspot emission as the native probe. We demonstrate a super-resolution microscopy investigation based on this idea. By modulating hotspot emission of crossed silver nanowires, which have a pair of plasmonic hotspots approximately 100 nm apart at the intersection, we precisely locate and separate them with nanometer precision. This label-free technique could be applied for analyzing hotspot distribution with high efficiency and precision.


Assuntos
Microscopia , Nanoestruturas , Corantes Fluorescentes
7.
Int J Cancer ; 2022 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-35037241

RESUMO

Carcinogenesis is one of major complications for patients with inflammatory bowel disease (IBD) and causes poor prognosis. We aimed to describe cancer incidence in Chinese IBD cohort compared with general population-based cancer registration data and further explore associated risk factors for cancer occurrence in IBD patients. IBD inpatients from January 1998 to January 2018 were included in this study. Patients were followed up from date of IBD diagnosis until either the date of first cancer diagnosis or January 2019. Standardized incidence ratios (SIRs) of overall cancer and site-specific cancers were calculated. A total of 869 UC and 516 CD patients were finally included with median follow-up time of 7 and 5 years respectively. 53 cases developed malignancies. After standardization by age and gender, standardized incidence ratio (SIR) of total cancer occurrence in IBD patients was 1.77 (95%CI, 1.33-2.32). As for UC, digestive cancers (SIR 3.75; 95%CI, 2.29-5.80), thyroid cancer (SIR 10.34; 95%CI, 4.72-19.64) and hematological malignancies (SIR 6.25; 95%CI, 1.68-16.00) had the highest incidence, which were prominent in young and middle-aged patients. Use of steroids, immunosuppressants or infliximab did not present higher risk of malignancies in UC patients. There were no significant difference in cancer risk between CD patients and general population. In conclusion, the increased risks of multiple cancers are particularly prominent in Chinese UC patients and these findings can provide more targeted guidance for cancer monitoring in Chinese IBD patients.

8.
Inflamm Bowel Dis ; 2022 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-34984462

RESUMO

BACKGROUND: Cytomegalovirus (CMV) causes infection in patients with inflammatory bowel disease (IBD). This study investigated the prevalence of CMV colitis, the current status of laboratory testing equipment, and physicians' opinions regarding CMV and IBD in China. METHODS: This retrospective multi-center study was conducted by Chinese members of the Asian Organization for Crohn's and Colitis and included 36 hospitals/institutes divided according to municipality, provincial capital city, and prefectural-level city. A survey questionnaire was administered, and chi-square and Fisher's exact tests were performed. RESULTS: A total of 4 823 inpatients with ulcerative colitis (UC) and 4.622 inpatients with Crohn's disease (CD) were included. The percentages of patients with moderate UC in the provincial capital city and municipality were significantly higher than that in the prefectural-level city (38.3% vs. 29.1% and 40.1% vs. 29.1%, respectively). The percentage of patients with mild CD was significantly lower in the provincial capital city than in the prefectural-level city and municipality (30.4% vs. 40.3%; 30.4% vs. 39.3%, respectively). There were 3.1% patients with UC and 0.8% patients with CD who had CMV colitis. The prevalence of CMV colitis was lower in patients with CD than in patients with UC (0.8% vs. 3.1%). Of the 150 patients with UC and concurrent CMV colitis, 17.3% patients underwent surgery, 2.0% died, and 23.3% experienced complications. Punched-out ulcerations were the major characteristic features for detecting CMV colitis. Approximately 77.8% of hospitals possessed testing facilities capable of conducting CMV immunohistochemistry. CONCLUSIONS: CMV colitis is an important issue during the disease progression of IBD. However, improvement in knowledge and facilities is required to enhance the prognosis of patients.

9.
Public Health Rep ; : 333549211061328, 2022 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-35067110

RESUMO

OBJECTIVES: Given several efforts to improve health care access in California and nationally, we studied whether linguistic and socioeconomic disparities in health care access changed from 2011 to 2019 among Asian and Pacific Islander American (APIA) people in Los Angeles, California. METHODS: We analyzed survey responses from APIA health fair participants (n = 5032) in the Greater Los Angeles area from May 15, 2011, through October 20, 2019. To assess the effect of socioeconomic factors on and shifts in health care access (ie, health insurance status/regular doctor), we used a logistic regression model. Covariates included English proficiency, year, age, sex, ethnicity, income, employment, and education. We also ran an interaction analysis between English proficiency and year. RESULTS: Health insurance access increased and doctor access remained stable throughout the study period; however, disparities in health insurance status widened by a factor of 1.08 per year between participants with high English proficiency (HEP) and participants with low English proficiency (LEP) (P = .01). People with HEP were 2.02 times more likely to have a regular doctor than people with LEP (P < .001), and this disparity persisted from 2011 to 2019 (P = .58). Participants who were young (P < .001) and male (P = .005) were significantly less likely to have health insurance and a regular doctor (P < .001) than participants who were older and female. Chinese participants were significantly more likely than Thai (P = .002) and Korean (P < .001) participants to be insured but not more likely to have a regular doctor, when controlling for health insurance. CONCLUSIONS: Policy changes targeting language and cultural barriers to care for APIA people with LEP may address the disparities observed.

10.
Mitochondrial DNA B Resour ; 7(1): 217-218, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35071761

RESUMO

Hibiscus coccineus is famous for its wide geographical distribution and the showy flowers of scarlet rose mallow. It belongs to the Malvaceae family and has greatly ornamental and ecological value. In this study, high-throughput sequencing and bioinformatics technology were used to assemble the complete chloroplast genome sequence, which will provide more genomic information for studying the genetic diversity and phylogenetic relationship. The full length of chloroplast genome is 160,280 bp, composed of a large single-copy (LSC) region of 89,121 bp, a small single-copy (SSC) region of 18,673 bp, and two inverted repeats (IRs) of 26,243 bp. A total of 113 genes were annotated, including 79 protein-coding genes, 30 tRNA, and four rRNA genes. Phylogenetic tree analysis revealed that the Hibiscus coccineus is closest to Hibiscus mutabilis in the Hibiscus L.

11.
Ginekol Pol ; 2022 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-35072240

RESUMO

OBJECTIVES: Our objective was to present the experience on prenatal diagnosis of 1p36 terminal deletion, and further delineated the fetal presentation of the syndrome. MATERIAL AND METHODS: This was a retrospective analysis of three new prenatal cases with pure 1p36 terminal deletion detected by chromosome microarray analysis (CMA) at a single Chinese medical center. We also reviewed 11 published prenatal cases with similar deletion sizes. Clinical data of all cases including indications for invasive testing, sonographic findings, maternal factors, and pregnancy outcomes were reviewed and analyzed. RESULTS: Three new cases with pure 1p36 terminal deletion were prenatal diagnosed by CMA, the sizes of the deletion were 1.3 Mb, 5.0 Mb, and 4.9 Mb respectively. All cases were detected because of abnormal ultrasound findings, including central nervous system (CNS) abnormalities, congenital heart disease (CHD) and fetal growth restriction. Two pregnancies were terminated, and one was live-born but died three months after birth. CONCLUSIONS: The 1p36 terminal deletion results in many clinical manifestations, but the specificity of clinical features are not high. Prenatal sonographic findings such as CNS, CHD may act as suggestive signs of 1p36 deletion or other microdeletion/duplication syndromes.

12.
Water Res ; 211: 118030, 2022 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-35065341

RESUMO

It is essential to understand the mechanism of algal bloom and develop effect measures to control the hazard in aquatic environment, such as large reservoirs. In this study, a series of experiments, along with field observation from 2007 to 2016, were carried out to identify the hydrodynamic parameters that drive the algal bloom in the Three Gorges Reservoir (TGR), China, and their threshold values were determined. The results show that algae concentration was markedly diluted with a short retention time, and the threshold value of the retention time to avoid algal bloom was approximately less than 3 days. With strong stratification, the algae concentration was able to approach to the level of algal bloom in 10 days, even when the water temperature is lower than 12 °C. The ratio of mixing depth to euphotic depth (Zm/Ze) had significant negative correlations with both algae concentration and algae specific growth rate (SGR). The field monitoring data indicated that Zm/Ze is an important hydrodynamic parameter which sensitively affects algae growth and concentration. This study made the first attempt to determine Zm/Ze >2.8 to restrain algal bloom in the TGR. Our findings shed light on the influence of critical depth on the algal bloom in the TGR, and the results can serve to control algal bloom in reservoirs through discharge operation.

13.
Phys Chem Chem Phys ; 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-35005759

RESUMO

Membrane permeation and the partitioning of polycyclic aromatic hydrocarbons (PAHs) are crucial aspects affecting their carcinogenicity and mutagenicity. However, a clear understanding of these processes is still rare due to the difficulty of determining the details experimentally. Here, the interactions between PAHs and lipid bilayers were studied by molecular simulations, mainly to check the influence of molecular weight and orientation. The liposome-water partition coefficient (KLW), transmembrane time (τ), and permeability coefficient (P) of the PAHs were calculated by integrating free energy profiles from umbrella sampling. For selected PAHs, the membrane adsorption is a spontaneous process. The preferred location is near the CC bond and the orientation is related to the molecular structure. The P values of all the PAHs are basically the same order of magnitude, which means that the molecular weight contributes little to the process. As for KLW and τ, they show obvious increases with different molecular weights. Unconstrained simulations showed that a flat orientation on the membrane surface would prevent PAHs from being transported through the membrane. Highly hydrophobic driving forces are not always good for the absorption of PAHs, especially the formation of aggregates. In addition, the orientations and energetic barriers of PAHs near the midplane of the lipid bilayer explain the different transitions of high- and low-weight PAHs. This work provides molecular level details relating to the interactions of PAHs with lipid membranes, with significance for understanding the health effects of PAHs.

14.
Hepatology ; 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-35007334

RESUMO

The study objective was to compare the effectiveness of microwave ablation (MWA) and laparoscopic liver resection (LLR) on solitary 3-5cm HCC over time. From 2008 to 2019, 1,289 patients from 12 hospitals were enrolled in this retrospective study. Diagnosis of all lesions were based on histopathology. Propensity score matching (PSM) was used to balance all baseline variables between the two groups in 2008-2019 (n =335 in each group) and 2014-2019 (n =257 in each group) cohorts, respectively. For cohort 2008-2019, during a median follow-up of 35.8 months, there were no differences in overall survival (OS) between MWA and LLR (Hazard ratio (HR): 0.88, 95% confidence interval (CI) 0.65-1.19, P =0.420), and MWA was inferior to LLR regarding disease-free survival (DFS) (HR 1.36, 95%CI (1.05-1.75), P =0.017). For cohort 2014-2019, there was comparable OS (HR 0.85, 95%CI (0.56-1.30), P =0.460) and approached statistical significance for DFS (HR 1.33, 95%CI (0.98-1.82), P =0.071) between MWA and LLR. Subgroup analyses showed comparable OS in 3.1-4.0cm HCCs (HR 0.88, 95%CI (0.53-1.47), P =0.630) and 4.1-5.0cm HCCs (HR 0.77, 95%CI (0.37-1.60), P =0.483) between two modalities. For both cohorts, MWA shared comparable major complications (both P >0.05), shorter hospitalization and lower cost to LLR (all P <0.001). Conclusion: MWA might be a first-line alternative to LLR for solitary 3-5cm HCC in selected patients with technical advances, especially for patients unsuitable for LLR.

15.
Eur J Clin Pharmacol ; 2022 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-35013802

RESUMO

PURPOSE: The full potential of methadone maintenance treatment (MMT) is often limited by the large inter-individual variability in both pharmacokinetics (PK) and pharmacodynamics (PD), and by the risk of torsade de pointes, a severe adverse effect caused by QTc prolongation. The current study aims to quantitate the contribution of genetic polymorphisms and other variables in PK/PD variability, and their contribution to the QTc interval prolongation in Chinese MMT patients. METHODS: Population PK models were developed to fit (R)- and (S)-methadone PK data. Hierarchical models were tested to characterize the PK profile, the concentration-QTc relationship, and concentration-urinalysis illicit drug testing relationship, with demographics and genetic variants being included as covariates. Simulation based on the developed PK/PD models was performed to assess the effect of methadone dose and genetic variants on QTc interval prolongation. RESULTS: The PK data were best-fit by a one-compartment, first-order absorption model. Clearance of (R)- and (S)-methadone was both affected by the weighted activity score derived from genetic variants. A linear model was used to describe both the methadone concentration-urinalysis illicit drug testing relationship and the methadone concentration-QTc relationship. Concentration of (R)- and (S)-methadone exhibits a comparable effect on QTc prolongation. Simulation showed that the percentage of QTc higher than 450 ms was almost doubled in the lowest clearance group as compared the highest when methadone dose was greater than 120 mg. CONCLUSIONS: The large variability in PK/PD profiles can be partially explained by the genetic variants in an extent different from other population, which confirmed the necessity to conduct such a study in the specific Chinese patients.

17.
J Environ Manage ; 305: 114404, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-34991024

RESUMO

Removal of nutrients in water is crucial to control eutrophication. Fly ash has been increasingly used to synthesize zeolite to remove nutrients, but it is still poorly understood about the removal capacity of zeolite synthesized from coal gasification slag (CGS), which has not been well recycled in many countries. In this study, the CGS was acid leached, alkali dissolved, and synthesized to carbon/zeolite composite (C/ZC) under induction by medical stone. After being modified by ferric sulfate, the composite was analyzed for the adsorption of NH4+ and PO43-. Results showed that the maximum adsorption capacity by C/ZC is 5.17 mg/g, but C/ZC has no adsorption capacity of PO43-. The ferric sulfate was used to modify C/ZC to obtain carbon/zeolite composite modified by iron (M-C/ZC). M-C/ZC has a higher specific surface area (348.3 m2/g), and the negatively charge of M-C/ZC can adsorb NH4+ and form Fe-O-P between PO43- and Fe-OH bonds. The maximum adsorption capacity of NH4+ and PO43- by M-C/ZC are 7.44 mg/g and 6.94 mg/g, respectively. The removal efficiency of NH4+ and PO43- are up to 88% and 99% under initial NH4+ (5 mg/L) and PO43- (10 mg/L) concentration. The regeneration capacity of M-C/ZC of NH4+ was stronger than that of PO43-. After three cycles, the regeneration rate of M-C/ZC of NH4+ was still up to 76.96%. Our findings suggest the good application potential of M-C/ZC for removing NH4+ and PO43- from wastewater.

18.
Bioresour Technol ; 347: 126623, 2022 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-35017093

RESUMO

The treatment performance of anaerobic ammonia oxidation (anammox) immobilized filler on different proportions of domestic wastewater was evaluated. The results showed that, in comparison to synthetic wastewater, 50% domestic wastewater promoted the anammox reaction of immobilized filler, while 100% domestic wastewater had no significant effect on the anammox activity of immobilized filler but the total nitrogen removal efficiency (TNRE) was improved through enhanced denitrification. The TNRE of the immobilized filler was 82.5%, which was significantly higher than that of AnGS (69.7%), and its average anammox contribution rate was more than 90%. This was because the encapsulated anammox biomass could better maintain competitive advantages and coordinate the symbiotic relationship with denitrifying bacteria. Moreover, lower NH4+-N concentration resulted in greater influence of C/N ratio on anammox performance than COD concentration, while the opposite was true at high NH4+-N concentration. This study verified that anammox immobilized filler is effective for mainstream applications.

19.
Process Saf Environ Prot ; 157: 1-19, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34744323

RESUMO

Establishing an accurate and efficient prediction model is of great significance for governments and other social organizations to formulate prevention and control policies and curb the explosive spread of the pandemic. To improve prediction accuracy of cumulative COVID-19 confirmed data, a new hybrid prediction model based on gradient-based optimizer variational mode decomposition (GVMD), extreme learning machine (ELM), and autoregressive integrated moving average (ARIMA), named GVMD-ELM-ARIMA, is proposed. To solve the problem of selecting the k value and the penalty factor α in variational mode decomposition (VMD), this paper proposes gradient-based optimizer variational mode decomposition (GVMD), which realizes the self-adaptive determination of k value and α value. Firstly, GVMD decomposes the cumulative COVID-19 confirmed data into some intrinsic mode functions (IMFs) and a residual component (IMFr). Secondly, IMFs are predicted by ELM. Then, IMFr is predicted by ARIMA. Finally, the final prediction results are obtained by reconstructing the prediction result of IMFs and IMFr. The cumulative COVID-19 confirmed data of the United States, India and Russia is used to verify its effectiveness. Taking the United States as an example, compared with the average MAPE, RMSE and MAE of the single model, the average MAPE of the hybrid model is reduced by 47.27%, the average RMSE is reduced by 44.50%, and the average MAE is reduced by 55.34%. Compared with GVMD-ELM-ELM, GVMD-ELM-ARIMA proposed in this paper reduces the MAPE by 60%, the RMSE by 56.85%, and the MAE by 61.61%. The experimental results show that GVMD-ELM-ARIMA has best prediction accuracy, and it provides a new method for predicting the cumulative COVID-19 confirmed data.

20.
Oncol Lett ; 23(1): 28, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34868365

RESUMO

Oral squamous cell carcinoma (OSCC) is the most commonly occurring oral malignancy. Cancer stem cells (CSCs) are known to be responsible for cancer recurrence and metastasis. Zinc-finger protein 750 (ZNF750) has been reported to inhibit OSCC cell proliferation and invasion. The present study aimed to elucidate the role of ZNF750 in the inhibition of the renewal ability of CSCs derived from the OSCC cell line, CAL-27. The effects of ZNF750 on CSC-like properties were examined using aldehyde dehydrogenase (ALDH), tumor sphere formation and colony formation assays. Reverse transcription-quantitative PCR and western blotting were performed to detect the expression levels of octamer-binding transcription factor 4, sex-determining region Y-box 2, the enhancer of zeste homolog 2 (Ezh2), embryonic ectoderm development (EED) and SUZ12 polycomb repressive complex 2 subunit (SUZ12), and for the identification of genes associated with metastasis. ZNF750 effectively attenuated CSC-like cell self-renewal abilities; ZNF750 decreased the ALDH-positive cell population, tumor sphere and colony formation abilities, cell viability and stemness factors. Furthermore, the expression levels of Ezh2, EED and SUZ12 were decreased by ZNF750. ZNF750 inhibited MMP1, 3, 9 and 13 expression levels, and decreased the cell invasion and migratory abilities. Moreover, the expression of tissue inhibitors of matrix metalloproteinases-1 was increased by ZNF750. However, opposite effects were observed following the knockdown of the ZNF750 gene. Overall, the present study demonstrated that ZNF750 has the potential to inhibit the renewal of CSC-like cells enriched from parental CAL-27 cells.

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