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1.
IEEE Trans Vis Comput Graph ; 26(1): 397-406, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31425037

RESUMO

Tennis players and coaches of all proficiency levels seek to understand and improve their play. Summary statistics alone are inadequate to provide the insights players need to improve their games. Spatio-temporal data capturing player and ball movements is likely to provide the actionable insights needed to identify player strengths, weaknesses, and strategies. To fully utilize this spatio-temporal data, we need to integrate it with domain-relevant context meta-data. In this paper, we propose CourtTime, a novel approach to perform data-driven visual analysis of individual tennis matches. Our visual approach introduces a novel visual metaphor, namely 1-D Space-Time Charts that enable the analysis of single points at a glance based on small multiples. We also employ user-driven sorting and clustering techniques and a layout technique that aligns the last few shots in a point to facilitate shot pattern discovery. We discuss the usefulness of CourtTime via an extensive case study and report on feedback from an amateur tennis player and three tennis coaches.

2.
Sci Total Environ ; 698: 134010, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31505337

RESUMO

The use of fertilizers as addition inputs in agricultural systems can increase the yield of wheat and maize, while also stimulating the emission of carbon dioxide from soil that the main man-made greenhouse gas. Our objectives focused on the impact of different types of synthetic fertilizers and water quality. The purposes were to determine the feasibility of using wasted water for irrigation and to relate CO2 fluxes to the yield of maize and wheat, as well as to select the best fertilizer type with low CO2 emission and high yield. The experiment consisted of a double factors test focusing on four forms of fertilizer (urea, amine and slow release fertilizer) and the quality of water (reclaimed water and underground water). The results showed that the reclaimed water was not significant on the CO2 discharge rate, the maize-wheat yield or the soil properties in 2014 or 2015; however, the CO2 emission increased slightly in 2015. Focusing on fertilizer treatments, the reclaimed water & amine fertilizer treatment (CAF) that had higher cumulative CO2 emissions was 32.75 t·ha-1 in 2014 and 33.86 t·ha-1 in 2015. According to the ratio CO2/Y, the slow released fertilizer that reduces CO2 emissions and keeps the yield high is the preferred choice.

3.
J Virol Methods ; 275: 113757, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31669331

RESUMO

The outbreak of an infectious disease characterized by severe symptom of gout has set great threat to several major goose-producing regions in China since December 2016. The causative agent for the novel infection has been identified was a novel goose-origin astrovirus (GoAstV). Lack of effective detection methods indeed hinders further research, as well as prevention and control of GoAstV. Keep this in mind, a TaqMan-based one-step real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) assay for rapid detection of GoAstV was developed. Primers and probe were targeting the capsid protein gene sequence (ORF2). The method is capable of detecting quite low number of targeting nucleic acid as low as 10 copies/µL. What's more, it is also of great specificity and repeatability for GoAstV detection. No cross-activity was found with other goose-origin viruses. The assay had excellent intra-assay and inter-assay repeatability with the coefficient of variation (CV) value from 0.48% to 0.99%. A total of 340 GoAstV specimens from different regions of China were used in this study to verify the feasibility and effectiveness of this method in clinical diagnosis. The results indicated that qRT-PCR is a highly sensitive, specific and repeatable method for quantitative detection of GoAstV, which can be used to detect this virus, thereby facilitating epidemiological investigations of gout in goslings.

4.
Enzyme Microb Technol ; 132: 109428, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31731949

RESUMO

Phenylalanine aminomutase (PaPAM) from Pantoea agglomerans is a member of the MIO (4-methylene-imidazol-5-one) family of enzymes, which isomerizes α-phenylalanine to ß-phenylalanine, and could be used to synthesize unnatural ß-arylalanine. However, the mechanism of isomerization reaction is not clear. To investigate the mechanism, the gene (pam), which encodes PaPAM, was first expressed in E.coli, and recombinant PaPAM was prepared using affinity chromatography. Then, 15N-(2S)-α-phenylalanine, (2S)-(3-2H2)-α-phenylalanine and (2S,3S)-[2,3-2H2]-α-phenylalanine were used as substrates to analyze the mechanism of isomerization reaction. The results of MS and NMR showed that the isomerization reaction was performed through the intramolecular exchange of NH2 with pro-3R hydrogen of α-phenylalanine. The PaPAM shuttles the α-NH2 of α-phenylalanine to ß site to replace the pro-3R hydrogen. Simultaneously, the pro-3R hydrogen is shifted to α site to produce ß-phenylalanine. Furthermore, a key residue, Phe at position 455 in the active site, was determined to control the exchange way using molecular docking and sequence alignment of MIO family enzymes. The results indicated that the key 455 Phe residue is involved in changing the binding orientation of the carboxyl group of the intermediate trans-cinnamic acid to control the NH2-H pair exchange.

5.
J Nanosci Nanotechnol ; 20(5): 3105-3116, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31635654

RESUMO

The Z-scheme Bi2WO6/NaBiO3 nanocomposites were first fabricated by a facile hydrothermal method, and were then characterized by X-ray diffraction, transmission electron microscopy, scanning electron microscopy, energy dispersive spectrometer, Fourier-transform-infrared spectroscopy, X-ray photoelectron spectroscopy and N2 adsorption-desorption. The as-prepared Bi2WO6/NaBiO3 nanocomposites exhibit outstanding photocatalytic activity and recyclability. A 98.4% photodegradation of 2,3-dichlorophenol (50 mg·L-1) was attained in the presence of Bi2WO6/NaBiO3 (1:10) under the visible-light irradiation in 30 min. In particular, the photocatalytic mechanism has been discussed in detail, based on four aspects: (1) oxidative species, (2) photoelectrochemical performance, (3) conduction band and valence band energy levels and (4) possible transition states and reactions. In conclusion, O-2 is the main active oxidative species in the Bi2WO6/NaBiO3 nanocomposite. The material has higher photocurrent and visible light adsorption but lower electron-hole pairs recombination, which contributes to distinguished photocatalytic efficiency. The Z-scheme photocatalytic path was proposed and the possible degradation process and routes have been summarized.

6.
Artigo em Inglês | MEDLINE | ID: mdl-31674893

RESUMO

Two strains, designated 2251T and 3058, that were aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli, have recently been isolated from Tibetan antelopes on the Qinghai-Tibet Plateau. The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %). Results of genomic sequence-based phylogenomic analysis agreed with those from 16S rRNA gene sequence analysis. Optimal growth was achieved at pH 7.0-7.5 and 28 °C with marine medium. Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone. The polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phospholipid, glycolipid and an unidentified lipid. The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose. The G+C content of strain 2251T was 66.5 mol%. Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database. Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp. nov. is suggested. The type strain is 2251T (=CGMCC 1.16490T=DSM 106269T).

7.
Fitoterapia ; 139: 104400, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31669962

RESUMO

Two novel epimer pairs of acetaminophen derivatives penicilquei A-D (1-4) were isolated from Penicillium herquei JX4. Their structures were elucidated using comprehensive spectroscopic methods. The absolute configurations of penicilquei A-D (1-4) were determined by modifified Mosher's method, and comparing their experimental and calculated electronic circular dichroism (ECD) spectra. Penicilquei A-D (1-4) are the first example of acetaminophen derivatives featuring an unprecedented carbon skeleton. The inhibitory activities of all compounds against nine phytopathogenic fungi and α-glucosidase were evaluated. Penicilquei A-D (1-4) showed strong inhibitory activities against at least eight phytopathogenic fungus.

8.
Sci Total Environ ; 703: 134487, 2019 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-31726294

RESUMO

Hydrochar, a solid hydrate with a high energy density, is produced by hydrothermal carbonization of lignocellulosic biomass and is widely applied in agriculture as a soil amendment. The fate and transport of hydrochar when applied to soil need to be investigated. The major components of soil, clay and phosphate, likely interact with hydrochar in the subsurface. This study investigated the cotransport behavior of hydrochar and diatomite (D) through water-saturated quartz sand in the presence of montmorillonite (M) and/or phosphate in NaCl (1-50 mM) solutions at pH 6.0 and 9.0. The transmission electron microscopy (TEM) and scanning electron microscopy (SEM) images and zeta potential (ZP) results showed that hydrochar-D nanoaggregates formed preferentially due to surface charge heterogeneity. M inhibited the transport of hydrochar-D in sand columns regardless of the solution pH mainly because the organo-mineral clusters of hydrochar-D with M were prone to filling the pores of the sand medium. Moreover, fine M particles preferentially attached to sand could decrease the ZP of the sand surface and subsequently decrease the repulsive forces between hydrochar-D and sand. The copresence of M and phosphate slightly facilitated hydrochar-D transport at pH 6.0 due to phosphate adsorption, whereas a negligible effect on transport occurred at pH 9.0. Thus, phosphate played a predominant role in the transport of hydrochar when clays were also present. A two-site kinetic retention model suggested that k1d/k1 and k2 are responsible for hydrochar-clay aggregate deposition in sand. Our findings relate to the potential risks posed by hydrochar in subsurface soils and aquifers where clay and phosphate ubiquitously co-occur.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31773943

RESUMO

The strong rigidity, low thermal conductivity and short of multi-driven capabilities of form-stable phase change materials (FSPCMs) have limited their practical utilizations. Herein, we report a shape-adaptable FSPCM with the coinstantaneous light/electro-driven shape memory property and light/electro-to-thermal energy storage performance. The FSPCM is fabricated by incorporating the polyethylene glycol (PEG) into the cellulose nanofiber/graphene nanoplatelet hybrid coated melamine foam (CG@MF). The CG@MF/PEG FSPCMs show good encapsulation effect, enhanced thermal conductivity, and large melting enthalpy (178.9 J g-1). Due to the high elasticity of MF and the excellent photothermal conversion and electrical conductivity of GNP network, the CG@MF/PEG FSPCMs exhibit remarkable light/electro-driven shape memory effect by activating the phase change process of PEG. Meanwhile, the CG@MF/PEG FSPCMs can effectively convert light or electric energy into heat energy and reposit the converted energy during the phase change process. Furthermore, the CG@MF/PEG FSPCMs possess excellent multiresponsive self-adhesion property. A light-sensitive, shape-adaptable and thermal-insulating container is further explored. This study provides routes towards the development of multiresponsive shape-adaptable FSPCMs for energy storage applications.

10.
Zhongguo Dang Dai Er Ke Za Zhi ; 21(11): 1099-1104, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31753092

RESUMO

OBJECTIVE: To investigate the current status of neglect in children aged 3-6 years in the rural areas of Urumqi, China and risk factors for neglect. METHODS: Stratified random cluster sampling was performed to select 1 326 children, aged 3-6 years, from 6 kindergartens in the rural areas of Urumqi. A self-designed questionnaire and "Chinese Neglect Evaluation Questionnaire for Rural Children Aged 3-6 Years" were used for investigation. RESULTS: Of the 1 326 children, the neglect rate was 51.89% (688 cases), and the degree of neglect was 49±6. There were significant differences in the neglect rate and the degree of neglect between the children in different age groups (P<0.01). The single-parent families and the remarried families had significantly higher child neglect rate and degree of neglect than the families of three generations under one roof and the core families (P<0.01). The children in left-behind status had a significantly higher degree of neglect (P<0.01). The multivariate logistic regression analysis showed that younger children, mothers with low education level, employment status of the mother as a migrant worker, single-parent and remarried families, low-income families, and left-behind status were risk factors for child neglect (P<0.05). CONCLUSIONS: The neglect of children aged 3-6 years in the rural areas of Urumqi is serious, and more attention should be paid to younger children, children whose mothers with low education level, left-behind children, and children from single-parent families, remarried families and low-income families.


Assuntos
Maus-Tratos Infantis , Criança , Pré-Escolar , China , Feminino , Humanos , Mães , Fatores de Risco , População Rural , Inquéritos e Questionários
11.
Vet Parasitol ; 276: 108991, 2019 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-31770701

RESUMO

Eimeria tenella, an obligate intracellular parasite, can actively invade the cecal epithelial cells of chickens and cause severe enteric disease. Eukaryotic elongation factor 2 (eEF2) plays a major role in protein synthesis and cell survival. This study aims to explore the exact mechanisms underlying diclazuril inhibition in second-generation merozoites of E. tenella. The eEF2 cDNA of the second-generation merozoites of E. tenella (EtEF2) was cloned by reverse transcriptase polymerase chain reaction and rapid amplification of cDNA ends. Diclazuril-induced expression profiles of EtEF2 were also analyzed. The cloned full-length cDNA (2893 bp) of the EtEF2 nucleotide sequence encompassed a 2499 bp open reading frame (ORF) that encoded a polypeptide of 832 residues with an estimated molecular mass of 93.12 kDa and a theoretical isoelectric point of 5.99. The EtEF2 nucleotide sequence was submitted to the GenBank database with the accession number KF188423. The EtEF2 protein sequence shared 99 % homology with the eEF2 sequence of Toxoplasma gondii (GenBank XP_002367778.1). The GTPase activity domain and ADP-ribosylation domain were conserved signature sequences of the eEF2 gene family. The changes in the transcriptional and translational levels of EtEF2 were detected through quantitative real-time PCR and Western blot analyses. The mRNA expression level of EtEF2 was 2.706 fold increases and the protein level of EtEF2 was increased 67.31 % under diclazuril treatment. In addition, the localization of EtEF2 was investigated through immunofluorescence assay. Experimental results demonstrated that EtEF2 was distributed primarily in the cytoplasm of second-generation merozoites, and its fluorescence intensity was enhanced after diclazuril treatment. These findings indicated that EtEF2 may have an important role in understanding the signaling mechanism underlying the anticoccidial action of diclazuril and could be a promising target for novel drug exploration.

12.
BMC Pulm Med ; 19(1): 225, 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31775708

RESUMO

BACKGROUND: This study was performed to analyze the clinical manifestations, imaging features, and prognosis of Takayasu's arteritis (TA) with pulmonary arteritis (PA). METHODS: In total, 51 of 815 patients with TA were diagnosed with PA at the Peking Union Medical College Hospital from 1986 to 2015. The patients' medical records and radiographic data were retrospectively reviewed. RESULTS: The patients comprised 39 women and 12 men with a median age of 33 years (range, 14-67 years). The most common symptoms were dyspnea (70.6%), cough (66.7%), hemoptysis (47.1%), and chest pain (45.1%). Computed tomography (CT) pulmonary angiography, pulmonary arteriography, and pulmonary perfusion imaging showed pulmonary artery stenosis or occlusion in 44 patients. A total of 82.4% of patients had lung parenchyma lesions on CT scans, indirectly indicating pulmonary artery involvement. Additionally, 58.8% of patients had pulmonary hypertension (PH) by echocardiography. Compared with the PH group, the non-PH group was characterized by a shorter disease duration; more symptoms such as fever, chest pain, and hemoptysis; an increased erythrocyte sedimentation rate; and a higher incidence of subpleural wedge-shaped shadows on chest CT (P < 0.05). The median follow-up period was 48 months (range, 1-212 months), and all three deaths occurred in the PH group. CONCLUSIONS: The clinical manifestations of TA with PA are nonspecific. PH often complicates PA and is associated with a poor prognosis. Early clinical manifestations such as repeated fever, chest pain, hemoptysis, and recurrence of subpleural wedge-shaped shadows on chest CT should arouse suspicion of PA in patients with TA and prompt further investigations. This may allow PA to be diagnosed before the occurrence of PH. TRIAL REGISTRATION: ClinicalTrials, NCT03189602. Date of registration: June 16, 2017. Retrospectively registered.

13.
Artigo em Inglês | MEDLINE | ID: mdl-31701732

RESUMO

The doping ring-core nickel phosphide/graphene nanomaterial is obtained by H2 reduction of the flower-like nickel phosphates/graphene oxide (NiPOGO) and sea urchin-like nickel phosphates/chemically converted graphene (NiPOG) substrates. The obtained structure of nickel phosphates depends on the influence of different kinds of oxygen-containing groups on the graphene substrate. The substrate can also affect the particle size and distribution of nickel phosphate nanoparticles. The substrate can adjust the particle size, distribution, and exposed growth direction of nickel phosphide. These materials with high activity are employed as electrochemical catalysts for methanol oxidation reactions, which is ∼7 times that of pure nickel phosphide, and there is a very small Tafel slope of 47 mV decade-1 in the water oxidation reaction. Our results highlight that the substrate structure is essential to catalytic materials for electrochemical oxidation of methanol and water.

14.
World J Pediatr ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31748985

RESUMO

BACKGROUND: The prognosis for relapsed or refractory acute myeloid leukemia (RR-AML) in children is poor, and the preferred salvage chemotherapy is unclear. One regimen is cladribine, cytarabine, and granulocyte-colony stimulating factor (CLAG), but little is known about its efficacy and safety in children with RR-AML. METHODS: We enrolled RR-AML patients aged 0-18 years who received modified CLAG regimen for re-induction between July 1, 2015 and April 1, 2018, or conventional induction between August 1, 2011 and April 1, 2018. Patients were followed up to March 31, 2019. Patients underwent allogeneic stem cell transplantation (allo-SCT) or chemotherapy after the induction of complete remission (CR). The CR rate, survival, and side effects were analyzed. RESULTS: The CR rate for induction was 66.7% after one cycle and 75.0% after two cycles of the CLAG regimen in 12 children. The nine children who received conventional chemotherapy had a CR rate of 22.2% after one cycle and 33.3% after two cycles (P = 0.087 vs. CLAG). The 3-year event-free survival (EFS) of the CLAG group and the conventional treatment group were 44.4 ± 15.7% and 22.2 ± 13.8% (P = 0.112). The 3-year overall survival of the two groups were 59.5 ± 16.2% and 22.2% ± 13.8% (P = 0.057). The 3-year EFS for allo-SCT and chemotherapy after CLAG regimen was 66.7 ± 19.2% and 25.0 ± 21.7% (P = 0.015). A single case of chemotherapy-related death was recorded. CONCLUSION: Our data suggest a promising CR rate using CLAG salvage treatment in childhood RR-AML. Allo-SCT after CR may improve the long-term outcome in these patients.

15.
Radiat Oncol ; 14(1): 206, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31752953

RESUMO

PURPOSE: To evaluate the short-term outcomes in terms of tumor control and toxicity of patients with skull base or cervical spine chordoma and chondrosarcoma treated with intensity-modulated proton or carbon-ion radiation therapy. METHODS: Between 6/2014 and 7/2018, a total of 91 patients were treated in our Center. The median age was 38 (range, 4-70) years. Forty-six (50.5%) patients were treated definitively for their conditions as initial diagnosis, 45 (49.5%) patients had recurrent tumors including 14 had prior radiotherapy. The median gross tumor volume was 37.0 (range, 1.6-231.7) cc. Eight patients received proton therapy alone, 28 patients received combined proton and carbon ion therapy, 55 patients received carbon-ion therapy alone. RESULTS: With a median follow-up time of 28 (range, 8-59) months, the 2-year local control (LC), progression free (PFS) and overall survival (OS) rates was 86.2, 76.8, and 87.2%, respectively. Those rates for patients received definitive proton or carbon-ion therapy were 86.7, 82.8, and 93.8%, respectively. On multivariate analyses, tumor volume of > 60 cc was the only significant factor for predicting PFS (p = 0.045), while re-irradiation (p = 0.012) and tumor volume (> vs < 60 cc) (p = 0.005) were significant prognosticators for OS. Grade 1-2 late toxicities were observed in 11 patients, and one patient developed Grade 3 acute mucositis. CONCLUSIONS: Larger tumor volume and re-irradiation were related to inferior survival for this group of patients. Further follow-up is needed for long-term efficacy and safety.

16.
Artigo em Inglês | MEDLINE | ID: mdl-31753647

RESUMO

Developing simple and sensitive non-aggregation strategy for detecting Cd2+ is necessary for improving the selectivity and sensitivity of probe. Here, we establish a simple, rapid and ratiometric strategy for the recognition of Cd2+ based on the formation of core-shell ZnSe/CdS structure using ZnSe quantum dots (QDs). The transformation from binary ZnSe QDs to core-shell ZnSe/CdS QDs both change the elemental composition and structure of ZnSe QDs, leading to the changes in band gap of ZnSe QDs, which could be observed in the UV-vis spectra. In the detection process, ZnSe QDs only possess absorption peak at 343 nm, the formation of ZnSe/CdS after the addition of Cd2+ leads to the appearance of the new peak at 397 nm, while other heavy metal ions could not cause the appearance of new absorption peak. Therefore, this strategy shows good selectivity for Cd2+ detection. Based on this strategy, the limit of detection (LOD) for Cd2+ is 11 nM by UV-vis spectroscopy with a desirable relation of linearity (R2 = 0.999) between A397/A343 and Cd2+ contents, which is superior to the LOD of most reported nanomaterials. The response time for Cd2+ detection is as short as 60 s, which is suitable for rapid detection. This ratiometric probe has also been applied to the detection of Cd2+ in tap water samples, the recovery of Cd2+ was between 94.9% and 105.6% for tap water samples, indicating the high accuracy of our ratiometric assay. Our strategy not only provided a new method for detecting Cd2+, but also put forward an implication that the band energy changes of QDs caused by heavy metal ions can be applied in the selective and sensitive detection of heavy metal ions.

17.
J Cell Mol Med ; 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31756048

RESUMO

Endometritis is a postnatal reproductive disorder disease, which leads to great economic losses for the modern dairy industry. Emerging evidence indicates that microRNAs (miRNAs) play a pivotal role in a variety of diseases and have been identified as critical regulators of the innate immune response. Recent miRNome profile analysis revealed an altered expression level of miR-148a in cows with endometritis. Therefore, the present study aims to investigate the regulatory role of miR-148a in the innate immune response involved in endometritis and estimate its potential therapeutic value. Here, we found that miR-148a expression in lipopolysaccharide (LPS)-stimulated endometrial epithelial cells was significantly decreased. Our results also showed that overexpression of miR-148a using agomiR markedly reduced the production of pro-inflammatory cytokines, such as IL-1ß and TNF-α. Moreover, overexpression of miR-148a also suppressed NF-κB p65 activation by targeting the TLR4-mediated pathway. Subsequently, we further verified that miR-148a repressed TLR4 expression by binding to the 3'-UTR of TLR4 mRNA. Additionally, an experimental mouse endometritis model was employed to evaluate the therapeutic value of miR-148a. In vivo studies suggested that up-regulation of miR-148a alleviated the inflammatory conditions in the uterus as evidenced by H&E staining, qPCR and Western blot assays, while inhibition of miR-148a had inverse effects. Collectively, pharmacologic stabilization of miR-148a represents a novel therapy for endometritis and other inflammation-related diseases.

18.
Biomed Pharmacother ; 120: 109546, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31675687

RESUMO

Cervical cancer (CC) is one of the most common cancers in women. However, copy number alteration (CNA)-driven dysregulated genes and their functions in CC are still rarely investigated. In the present study, we conducted integrative analysis of CNA and gene expression data from The Cancer Genome Atlas (TCGA) cervical cancer to identify dysregulated genes triggered by CNAs. The integration of copy number status and RNA expression revealed 763 amplified and 1,391 deleted genes significantly dysregulated by the CNAs (P-value < 1e-8). Among these CNA genes, five driver genes, including PI3KCA, PI3KCB, DVL3, WWTR1, and ERBB2, exhibited a strong association with immune cell infiltration, suggesting that the pathways that they participate in may be involved in regulating immune cell infiltration. Moreover, we also observed that the genes of immunotherapeutic targets were abundantly expressed in the wild-type samples, suggesting that immunotherapy based on these immunotherapeutic targets may be applied to wild-type samples. In addition, the two CNA driver genes, DVL3 and ERBB2, might be sensitive and resistant biomarkers for examining the tumor's response to chemoradiotherapy, respectively. Particularly, the expression of ERBB2 was also observed to be higher in responders of chemotherapy than non-responders. Furthermore, a subset of CNA genes was identified to predict the prognosis of cervical cancer. In summary, our systematic data analysis of these CNA genes not only improved our understanding of the veiled mechanism behind immune cell infiltration, but also provided the potential clinical application of these CNA genes in cervical cancer.

19.
Dalton Trans ; 48(45): 16974-16985, 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31687705

RESUMO

Herein, we have studied the crystallization of zeolite omega by the solid-state transformation of TMA-magadiite at 100 °C for 12 h. The samples prepared at different times were subjected to XRD, SEM, IR, Raman and solid MAS NMR analyses to investigate the crystallization behaviors and changes in the medium-range structure during the synthesis process and a comprehensive mechanism was proposed. It has been demonstrated that the 5Rs and 6Rs in magadiite are partially retained in the system and participate in the growth of zeolite omega. The 4Rs were formed after heating for 4 h. The synthesis time of the zeolite omega using this method is shorter than that using the magadiite hydrothermal conversion method (about 72 h), because special composition building units, which have similarities to the structure of zeolite omega, were formed and adsorbed on the surface of the TMA-magadiite, and then provided a growth surface for the synthesis of targets. In addition, recycling the waste mother liquid produced in the preparation of the precursor was done to achieve the low cost and green synthesis. Finally, several factors influencing the reaction are discussed.

20.
J Exp Bot ; 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31740933

RESUMO

The plant hormone abscisic acid (ABA) plays a crucial role during the plant life cycle as well as in adaptive responses to environmental stresses. The core regulatory components of ABA signaling in plants are the pyrabactin resistance1/PYR1-like/regulatory ABA receptors (PYLs for simplicity) comprise the largest plant hormone receptor family known. They act as negative regulators of members of the protein phosphatases type 2C family (PP2Cs). Due to the biological importance of PYLs, many researchers have focused on their genetic redundancy and consequent functional divergence. However, little is understood of their evolution and its impact on the generation of regulatory diversity. In this study, we identified positive selection and function divergency in PYLs through phylogenetic reconstruction, gene structure and expression pattern analysis, positive selection analysis, functional divergence analysis, and structure comparison. We found correlation of desensitization of PYLs with specific modifications in the molecular recognition domain with functional diversification. Hence, an interesting antagonistic coevolution mechanism is proposed, which results in the functional diversification of ABA receptor family proteins. We believe a compensatory evolutionary pathway may have occurred.

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