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1.
Biochim Biophys Acta Biomembr ; 1865(1): 184082, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36374760

RESUMO

Both metal ions and lipid membranes have a wide distribution in amyloid plaques and play significant roles in AD pathogenesis. Although influences of different metal ions or lipid vesicles on the aggregation of Aß peptides have been extensively studied, their combined effects are less understood. In this study, we reported a unique effect of copper ion on Aß aggregation in the presence of lipid vesicles, different from other divalent metal ions. Cu2+ in a super stoichiometric amount leads to the rapid formation of ß-sheet rich structure, containing abundant low molecular weight (LMW) oligomers. We demonstrated that oligomerization of Aß40 induced by Cu2+ binding was an essential prerequisite for the rapid conformation transition. Overall, the finding provided a new view on the complex triple system of Aß, copper ion and lipid vesicles, which might help understanding of Aß pathologies.


Assuntos
Peptídeos beta-Amiloides , Cobre , Cobre/química , Peptídeos beta-Amiloides/metabolismo , Metais , Íons , Lipídeos
2.
Sci Total Environ ; 855: 158652, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36108864

RESUMO

Due to the public health concern of arsenic, environmental management measures in mining areas had been implemented. To assess the effect of environmental management measures in the mining area comprehensively, arsenic accumulation in the urine, hair, nails, and urinary metabolites of residents in a realgar mining area in Hunan province, China were investigated in 2019, and the changes in arsenic levels in the biomarkers during 2012-2019 were tracked. The importance of confounding factors (age, sex, occupation, residence, clinical history, vegetable source, cooking fuel, smoking, alcohol consumption, BMI) was analyzed using the Boruta algorithm. After the implementation of environmental management measures (including ceasing mining and smelting activities, building landfills, adjusting the planting structure, and soil restoration), urine, hair, and nail arsenic concentration decreased drastically but were still excessive. Arsenic accumulation was highest in older male miners who were long settled in the mining area and consumed homegrown vegetables. The only factor for changes in urinary arsenic levels was the cooking fuel type; residents using wood as cooking fuel experienced sustained arsenic exposure. Occupation and sex were important for determining arsenic changes in the hair and nails. Short-term arsenic accumulation in urine was affected by arsenic exposure, while long-term accumulation in hair and nails by arsenic metabolic capacity. The percentage of urinary arsenic metabolism and arsenic methylation indices of the participants in the mining area were within the normal range (%iAs: 10-30 %, %MMA: 10-20 %, % DMA: 60-80 %); samples indicated worse metabolic capacity than the reference population. The arsenic metabolic capacity of male miners was relatively weak, probably aggravated by alcohol drinking and smoking. Without soil remediation, arsenic exposure will continue. Homegrown vegetables and biomass fuels should be abandoned; reduced cigarette and alcohol consumption is recommended. Urinary arsenic would be more proper for assessing environmental remediation in mining areas.


Assuntos
Arsênio , Humanos , Masculino , Idoso , Arsênio/análise , Conservação dos Recursos Naturais , Exposição Ambiental/análise , Mineração , Solo
3.
Sci Total Environ ; 857(Pt 1): 159119, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36183764

RESUMO

The lateral transport of heavy metals can expand the scope of original contamination, and an accurate prediction of heavy metal migration is necessary to control heavy metal transport. However, previous studies have mainly focused on the migration of soil pollutants in the runoff-soil-groundwater system, whereas research on the lateral migration of heavy metals in surface soil driven by rainfall is relatively scarce. Therefore, in this study we analyzed the horizontal migration of water-soluble heavy metals with surface runoff and non-water-soluble heavy metals with sediment particles, investigated the main factors affecting the processes of runoff and sediment transport and the main factors affecting the mobility of heavy metals in soils, summarized the existing methods for the simulation of heavy metal transportation. The construction of a lateral migration model based on the migration mechanism of soil heavy metals, the hydrological model, and the application of the lateral migration model should be the focus of future research. This study provides a theoretical basis for establishing a model of the lateral migration of soil heavy metals and is of great significance for the prevention and control of the risks related to the lateral migration of soil heavy metals.

4.
Sci Total Environ ; 856(Pt 1): 159004, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36155037

RESUMO

Cyanobacterial blooms have profound effects on the structure and function of plankton communities in inland waters, but few studies have focused on the effects of microbial-based processes in one­carbon and nitrogen cycling on water quality improvement following the bloom. Here, we compared the structure and function of the bacterial community, focusing on microbial one­carbon and nitrogen metabolisms during and after a cyanobacterial Microcystis bloom in a deep subtropical reservoir. Our data showed that microbial one­carbon and nitrogen cycles were closely related to different periods of the bloom, and the changes of functional genes in microbial carbon and nitrogen cycling showed the same consistent trend as that of Methylomonas sp. With the receding of the bloom, the abundance of Methylomonas as well as the functional genes of microbial one­carbon and nitrogen cycling reached the peak and then recovered. Our results indicate that microbial one­carbon and nitrogen metabolisms were beneficial to the recovery of water quality from the cyanobacterial bloom. This study lays a foundation for a deep understanding of the cyanobacterial decomposition mediated by microbes in one­carbon and nitrogen cycles in inland freshwaters.


Assuntos
Cianobactérias , Microcystis , Carbono/metabolismo , Cianobactérias/metabolismo , Microcystis/metabolismo , Água Doce/microbiologia , Nitrogênio/metabolismo , Eutrofização , Lagos/microbiologia
5.
Bioresour Technol ; 367: 128228, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36332868

RESUMO

Shortage of anaerobic ammonium oxidation (anammox) sludge greatly limits the extensive full-scale application of anammox-based processes. Although numerous start-up strategies have been proposed, the interaction among microbial consortia and corresponding mechanism during the process development remain unknown. In this study, three reactors were established based on different seed sludges. After 27 days, the anammox process inoculated with anammox granules and activated sludge (1:5) was firstly achieved, and the highest nitrogen removal rate was 1.17 kg N m-3 d-1. Correspondingly, the anammox activity and abundances of related functional genes increased. Notably, the dominant anammox bacteria shifted from Candidatus Kuenenia to Candidatus Brocadia. Metagenomic analysis indicated that quorum sensing-based regulation mainly contributed to the proliferation and accumulation of anammox bacteria. This work provides an insight into the quorum sensing (QS)-regulated microbial interactions in the anammox and activated sludge consortia during the process development.


Assuntos
Compostos de Amônio , Esgotos , Esgotos/microbiologia , Percepção de Quorum , Anaerobiose , Reatores Biológicos/microbiologia , Oxirredução , Nitrogênio , Bactérias/genética , Desnitrificação
6.
J Hazard Mater ; 442: 130085, 2023 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-36193611

RESUMO

Material-enhanced heterogeneous peroxymonosulfate (PMS) activation for degradation of antibiotic in water has attracted intensive attention. However, one challenge is the electron transfer efficiency from the material to PMS for reactive oxygen species (ROS) production. Considering that the B-sites of perovskite oxides are closely associated with the catalytic performance, partial substitution of the B-sites of perovskite oxides can enhance the redox cycle of metals. Consequently, adjusting the ratio of each element at the B site can introduce oxygen vacancies on the surface of perovskite. Herein, a method was developed in which manganese (Mn) partially substitutes B-sites to modify surface properties of SrCoO2.52 perovskite oxides, resulting in the enhancement of catalytic activity. In degradation kinetics studies using SrCoMnO3-δ-0.5/PMS (SrCoMnO3-δ-0.5 denotes that the molar substitution of Mn at the B site of SrCoO2.52 perovskite oxide is 0.5) reaction system and sulfamethoxazole (SMX) as the target pollutant, it was found that the reaction rate constant (kobs) is 0.287 min-1 which is 2.4 times that of SrCoO2.52/PMS system. Experimental and theoretical analyses revealed that Mn-O covalent bonding governs the intrinsic catalytic activity of SrCoMnO3-δ-0.5 perovskite oxides. The Mn sites exhibits stronger adsorption energy with PMS than the Co sites, facilitating the breaking of O-O bond. Simultaneously, oxygen vacancies and surface adsorbed oxygen species have a synergistic effect for PMS adsorption. This work can provide a potential route in developing advanced catalysts based on manipulation of the B-sites of perovskite oxides for PMS activation.


Assuntos
Poluentes Ambientais , Manganês , Espécies Reativas de Oxigênio , Peróxidos/química , Óxidos/química , Sulfametoxazol/química , Oxigênio , Água , Antibacterianos
7.
J Hazard Mater ; 443(Pt A): 130079, 2023 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-36242955

RESUMO

Compared with the widespread and serious heavy metal contamination in soils, microplastic pollution has gained attention only recently. Little is known about how microplastics affect the distribution of heavy metals in soils, especially across soil components level. In this study, a 180-day soil aging experiment and soil density fractionation were performed to investigate the effect of polypropylene (PP) microplastics on the binding behavior of cadmium (Cd) to solid components, i.e. particulate organic matter, organo-mineral complexes (OMC), and mineral. Results showed addition of 2-10% microplastics in soils induced the decomposition of OMC fraction by 10.88-23.10%. Compared to the control, the content of dissolved organic carbon increased, and pH, humic substances, and soil organic matter decreased with microplastics. After 180d of aging, the content of Cd in OMC fraction increased by 17.92%, while microplastics made Cd contents decline by 10.01-19.75%. The impacts strongly depended on the dose and surface characteristic of microplastics. Overall, PP microplastics increased the concentration of bioavailable Cd in soils via decreasing soil retention of Cd by the OMC fraction. These findings based on the solid components level will provide a new perspective for understanding microplastics effects on soil systems and pollutants.


Assuntos
Metais Pesados , Poluentes do Solo , Solo/química , Cádmio/química , Microplásticos , Plásticos/metabolismo , Polipropilenos , Disponibilidade Biológica , Poluentes do Solo/análise , Metais Pesados/análise , Material Particulado
8.
Meat Sci ; 195: 109019, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36335867

RESUMO

This study aimed to explore the effects of different levels (0, 8, and 12 kGy) of low-energy electron beam (LEEB; 0.2 MeV) on the quality and shelf-life of vacuum-packaged pork stored under chilled and superchilled conditions for 30 days. LEEB irradiation promoted lipid and protein oxidation, regardless of the levels used. Combined 8 kGy LEEB irradiation with superchilled storage significantly elevated a* values and preserved acceptable sensory characteristics of pork until day 30, unlike the other treatment combinations. This synergistic treatment also significantly reduced total viable counts and total volatile basic nitrogen contents, and thus extended the shelf-life of vacuum-packaged pork to >30 days, which may be due to the antibacterial effects on Photobacterium, Carnobacterium and Lactobacillus. In conclusion, it is recommended that the 8 kGy LEEB irradiation level be applied to pork and that it should be used in combination with superchilled storage to preserve meat quality and extend the shelf-life of vacuum-packaged pork.


Assuntos
Carne de Porco , Carne Vermelha , Animais , Suínos , Vácuo , Embalagem de Alimentos , Carne Vermelha/microbiologia , Microbiologia de Alimentos , Elétrons
9.
Neural Regen Res ; 18(5): 1118-1123, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-36255001

RESUMO

Studies have shown that human hair keratin (HHK) has no antigenicity and excellent mechanical properties. Schwann cells, as unique glial cells in the peripheral nervous system, can be induced by interleukin-1ß to secrete nerve growth factor, which promotes neural regeneration. Therefore, HHK with Schwann cells may be a more effective approach to repair nerve defects than HHK without Schwann cells. In this study, we established an artificial nerve graft by loading an HHK skeleton with activated Schwann cells. We found that the longitudinal HHK microfilament structure provided adhesion medium, space and direction for Schwann cells, and promoted Schwann cell growth and nerve fiber regeneration. In addition, interleukin-1ß not only activates Schwann cells, but also strengthens their activity and increases the expression of nerve growth factors. Activated Schwann cells activate macrophages, and activated macrophages secrete interleukin-1ß, which maintains the activity of Schwann cells. Thus, a beneficial cycle forms and promotes nerve repair. Furthermore, our studies have found that the newly constructed artificial nerve graft promotes the improvements in nerve conduction function and motor function in rats with sciatic nerve injury, and increases the expression of nerve injury repair factors fibroblast growth factor 2 and human transforming growth factor B receptor 2. These findings suggest that this artificial nerve graft effectively repairs peripheral nerve injury.

10.
Rev. bras. med. esporte ; 28(6): 676-678, Nov.-Dec. 2022. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1376728

RESUMO

ABSTRACT Introduction Running can promote health prevent obesity, cardiovascular, and cerebrovascular diseases, among other chronic diseases. Much research reports the benefits of this aerobic resistance exercise, but little is known about the impacts of running on knee osteoarthritis. Objective To observe and study the clinical effect of running on pain and function improvement in patients with knee osteoarthritis. Methods Seventy cases that met the inclusion criteria were selected and randomly divided into control and observation groups. Patients in the observation group received eight weeks of neuromuscular exercise (NEMEX) and eight weeks of quadriceps muscle strength training while the control group was subjected to a running protocol. Patients were assessed using the Western Ontario and McMaster Universities Pain Scale and Osteoarthritis Index (WOMAC) after 1, 2, 4 and 8 weeks of treatment. Results After 1, 2, 4, and 8 weeks of treatment, the WOMAC function scores of the two groups significantly improved compared to that found before treatment (P < 0.05); There was no difference between the two groups before treatment and after 1, 2 weeks of treatment. After 4 and 8 weeks of treatment, the function of the observation group was better than that of the control group; the difference was statistically significant (t= -4.287, -6.355, P<0.05). Conclusions NEMEX training methods and quadriceps muscle strength training can effectively improve patients' functional level, but NEMEX improves more significantly than quadriceps muscle strength training. Evidence level II; Therapeutic Studies - Investigating the results.


RESUMO Introdução A corrida pode promover a saúde, prevenir a obesidade, doenças cardiovasculares e cerebrovasculares entre outras doenças crônicas. Muitas pesquisas relatam os benefícios desse exercício de resistência aeróbica, mas pouco conhecimento há sobre os impactos da corrida na osteoartrite do joelho. Objetivo Observar e estudar o efeito clínico da corrida na melhora da dor e da função em pacientes com osteoartrite de joelho. Métodos Setenta casos que preencheram os critérios de inclusão foram selecionados e divididos aleatoriamente em grupos controle e observação. Os pacientes do grupo de observação receberam 8 semanas de exercício neuromuscular (NEMEX) e 8 semanas de treinamento de força muscular do quadríceps enquanto o grupo controle foi sujeitado a um protocolo de corrida. Os pacientes foram avaliados usando a Escala de Dor e o Índice de Osteoartrite das Universidades Western Ontario and McMaster (WOMAC) após 1, 2, 4 e 8 semanas de tratamento. Resultados Após 1, 2, 4 e 8 semanas de tratamento, os escores de função WOMAC dos dois grupos melhoraram significativamente comparados ao encontrado antes do tratamento (P <0,05). Não houve diferença entre os dois grupos antes do tratamento e após 1, 2 semanas de tratamento. Após 4 e 8 semanas de tratamento, a função do grupo de observação foi melhor que a do grupo controle, a diferença foi estatisticamente significativa (t= -4,287, -6,355, P<0,05). Conclusões Ambos os métodos de treinamento NEMEX e o treinamento de força muscular do quadríceps podem efetivamente melhorar o nível funcional dos pacientes, e o NEMEX melhora mais significativamente do que o treinamento de força muscular do quadríceps. Nível de evidência II; Estudos terapêuticos - Investigação de resultados.


RESUMEN Introducción La corrida puede promover la salud, prevenir la obesidad, enfermedades cardiovasculares y cerebrovasculares, entre otras enfermedades crónicas. Muchas investigaciones informan los beneficios de este ejercicio de resistencia aeróbica, pero se sabe poco sobre los impactos de correr en la osteoartritis de rodilla. Objetivo Observar y estudiar el efecto clínico de la corrida sobre el dolor y la mejora funcional en pacientes con artrosis de rodilla. Métodos Setenta casos que cumplieron con los criterios de inclusión fueron seleccionados y divididos aleatoriamente en grupos de control y observación. Los pacientes del grupo de observación recibieron 8 semanas de ejercicio neuromuscular (NEMEX) y 8 semanas de entrenamiento de fuerza de los cuádriceps, mientras que el grupo de control se sometió a un protocolo de corrida. Los pacientes fueron evaluados utilizando la escala de dolor y el índice de osteoartritis de las universidades de Western Ontario y McMaster (WOMAC) después de 1, 2, 4 y 8 semanas de tratamiento. Resultados Después de 1, 2, 4 y 8 semanas de tratamiento, las puntuaciones de función WOMAC de los dos grupos mejoraron significativamente en comparación con las encontradas antes del tratamiento (P < 0,05). No hubo diferencia entre los dos grupos antes del tratamiento y después de 1, 2 semanas de tratamiento. Después de 4 y 8 semanas de tratamiento, la función del grupo de observación fue mejor que la del grupo control, la diferencia fue estadísticamente significativa (t= -4.287, -6.355, P<0.05). Conclusiones Tanto los métodos de entrenamiento NEMEX como el entrenamiento de la fuerza muscular del cuádriceps pueden mejorar eficazmente el nivel funcional de los pacientes, y NEMEX mejora de forma más significativa que el entrenamiento de la fuerza muscular del cuádriceps. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

11.
Macromol Rapid Commun ; : e2200694, 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36412066

RESUMO

Poly(malic acid) (PMLA) is a water-soluble, biodegradable, biocompatible, and nontoxic polyester in the poly(hydroxyalkanoate) (PHA) family. it features various applications in pharmaceutical field. Here, NaCo(CO)4 and pyridine derivatives are employed for direct carbonylative polymerization of benzyl glycidate (BG) for poly(ß-malic acid) production. Further investigation on reaction mechanism reveals that this polymerization undergoes a direct chain growth, rather than a sequential process involving ß-lactone intermediate. The low cost and facile preparation of epoxide substrate render this methodology extremely appealing that avoids the rather tedious procedures for ß-malolactonate synthesis required toward ring opening polymerization. This study also represents an alternative strategy over traditional methods for poly(ß-malic acid) production using step growth polycondensation of malic acid.

12.
Front Neurol ; 13: 997217, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36388219

RESUMO

Objective: The present study aimed to investigate the status of vestibular function in children with vestibular migraine of childhood (VMC) reflected by vestibular function test battery and explore the pathophysiological implication of these instrument-based findings. Methods: The clinical data of 22 children (mean age 10.7 ± 2.9 years) with VMC who met the diagnostic criteria of the Barany Society were collected from September 2021 to March 2022. A vestibular function test battery on these children included a caloric test, video head impulse test (vHIT), cervical vestibular-evoked myogenic potential (cVEMP), and ocular vestibular-evoked myogenic potential (oVEMP); these parameters were triggered by air-conducted sound (ACS) and galvanic vestibular stimulation (GVS). The subjects were further divided into two groups: <3 months and >3 months according to the disease duration from symptom onset. The functional abnormalities and their characteristics reflected by the vestibular test battery, as well as the outcomes in children with or without aura, were analyzed. Results: (1) The abnormal rate of the caloric test was 15.8% and that of vHIT was 0%. The response rates of ACS-cVEMP and ACS-oVEMP were 100% and 90.5%, respectively. The response rates of GVS-cVEMP and GVS-oVEMP were 100% and 88.9%, respectively. (2) No statistical difference was observed in the abnormal rate of the caloric test (P = 0.55) and the response rate of ACS-oVEMP (P = 0.21) between the two groups, irrespective of the course duration. (3) No statistical difference was detected in the abnormal rate of the caloric test (P = 0.53) and the response rate of ACS-oVEMP (P = 1.00) in children with or without aura. Conclusion: Vestibular function status comprehensively reported by the vestibular test battery did not show an aggravation with the disease duration in children with VMC. Also, it was not affected by the existence of aura in children with VMC. The high abnormal rates of the caloric test and oVEMPs (ACS-oVEMP and GVS-oVEMP) suggested that the lateral semicircular canal (low-frequency function component), the utricle, and the superior vestibular conduction pathway might be involved in VMC.

13.
Front Plant Sci ; 13: 1013331, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36388515

RESUMO

Exploring the diversity and formation mechanism of under-ground bud banks is essential for understanding the renewal of plant populations and community succession. However, there are few studies on the response of bud bank size and composition to different degradation gradients in alpine meadows. In view of this, we investigated the size and composition of bud bank under four degradation gradients (non-degraded:ND, lightly degraded:LD, moderately degraded:MD, and heavily degraded:HD) caused by overgrazing in a typical alpine meadow in Tibet, China, using a unit area excavation sampling method, and analyzed the correlation between above-ground plant community composition and bud bank density. Our results showed that: (i) in the ND alpine meadow, rhizome buds were dominant, in the LD, tiller buds were dominant, and in the MD, root-sprouting buds were dominant; (ii) total bud bank and cyperaceae bud density decreased with increasing degradation gradient, the density of leguminosae was insignificant in each degradation gradient, and the density of gramineae and forb were dominant in LD and MD meadows, respectively; (iii) total bud bank density was significantly and positively correlated with total above-ground biomass in the LD gradient, tiller bud density was significantly positively correlated with the species diversity index of above-ground vegetation under the ND gradient, rhizome bud density was significantly and positively correlated with total above-ground biomass in the LD gradient, and root-sprouting density was significantly negatively correlated with total above-ground biomass in ND meadows, but was significantly positively correlated with the species diversity index of the LD gradient. Therefore, our research shows that rhizome buds are more important in ND meadow habitats, tiller buds are more important in LD meadow habitats, and root-sprouting buds are more important in MD meadows. The response of bud banks to degradation gradient varies with different types of bud banks and different functional groups of plants, and the survival strategy of bud banks is of great value for community restoration and regeneration, which should be paid more attention to in subsequent alpine meadow research.

14.
Front Plant Sci ; 13: 1022819, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36388559

RESUMO

Didymella leaf blight (DLB) caused by Didymella glomerata is a new fungal disease of maize (Zea mays), first detected in 2021 in Panjin, Liaoning province of China. Here we report the reference genome assembly of D. glomerata to unravel how the fungal pathogen controls its virulence on maize at the molecular level. A maize-infecting strain Pj-2 of the pathogen was sequenced on the Illumina NovaSeq 6000 and PacBio Sequel II platforms at a 575-fold genomic coverage. The 33.17 Mb gapless genome assembly comprises 32 scaffolds with L/N50 of 11/1.36 Mb, four of which represent full-length chromosomes. The Pj-2 genome is predicted to contain 10,334 protein-coding genes, of which 211, 12 and 134 encode effector candidates, secondary metabolite backbone-forming enzymes and CAZymes, respectively. Some of these genes are potentially implicated in niche adaptation and expansion, such as colonizing new hosts like maize. Phylogenomic analysis of eight strains of six Didymella spp., including three sequenced strains of D. glomerata, reveals that the maize (Pj-2)- and Chrysanthemum (CBS 528.66)-infecting strains of D. glomerata are genetically similar (sharing 92.37% genome with 98.89% identity), whereas Pj-2 shows truncated collinearity with extensive chromosomal rearrangements with the Malus-infecting strain M27-16 of D. glomerata (sharing only 55.01% genome with 88.20% identity). Pj-2 and CBS 528.66 carry four major reciprocal translocations in their genomes, which may enable them to colonize the different hosts. Furthermore, germplasm screening against Pj-2 led to the identification of three sources of DLB resistance in maize, including a tropical inbred line CML496. DLB resistance in the line is attributed to the accumulation of ROS H2O2 in the apoplastic space of the infected cells, which likely restricts the fungal growth and proliferation.

15.
BMJ Open ; 12(11): e062406, 2022 11 14.
Artigo em Inglês | MEDLINE | ID: mdl-36375972

RESUMO

OBJECTIVES: Primary aldosteronism (PA), the most common endocrine cause of hypertension, is associated with a higher risk of cardiovascular disease (CVD) than blood pressure (BP)-matched essential hypertension (EH). We aimed to compare the calculated risks of CVD in patients who had hypertension with PA or EH using CVD risk calculators, hypothesising that they will fail to recognise the increased CVD risk in PA. DESIGN: Cross-sectional analysis. SETTING: An endocrine hypertension service in Victoria, Australia. PARTICIPANTS: Patients who had hypertension without CVD referred for the investigation of hypertension. OUTCOME MEASURES: Calculated 5-year or 10-year CVD risk as predicted by the National Vascular Disease Prevention Alliance (NVDPA) algorithm, Framingham Risk Score, Pooled Cohort Equations and QRISK3. RESULTS: Those with PA (n=128) and EH (n=133), did not differ significantly in their calculated CVD risks with the NVDPA algorithm (moderate-to-high 5-year risk 36/100 vs 45/99, p=0.17); the Framingham Risk Score (median 10-year risk 7.72% (4.43%-12.95%) vs 6.84% (3.85%-10.50%), p=0.14); the Pooled Cohort Equations (median 10-year risk 9.45% (4.36%-15.37%) vs 7.90% (2.09%-14.73%), p=0.07); and QRISK3 (median 10-year risk 11.31% (7.22%-20.29%) vs 12.47% (5.10%-19.93%), p=0.51). Similarities persisted on regression analyses accounting for systolic BP. CONCLUSIONS: CVD risk algorithms do not reflect the increased risk of CVD in patients with PA, and likely underestimate the true risk of CVD among those with PA. Screening for PA, in addition to using the CVD risk algorithm in patients who had hypertension, may facilitate the targeted treatment of PA and minimisation of cardiovascular risk in affected individuals.


Assuntos
Doenças Cardiovasculares , Hiperaldosteronismo , Hipertensão , Humanos , Hipertensão Essencial/complicações , Hipertensão Essencial/epidemiologia , Hiperaldosteronismo/complicações , Hiperaldosteronismo/epidemiologia , Doenças Cardiovasculares/etiologia , Doenças Cardiovasculares/complicações , Estudos Transversais , Fatores de Risco , Hipertensão/complicações , Hipertensão/epidemiologia , Fatores de Risco de Doenças Cardíacas , Vitória
16.
Artigo em Inglês | MEDLINE | ID: mdl-36402359

RESUMO

PURPOSE: The standard dose of definitive concurrent chemoradiotherapy (dCRT) remains 50.4 Gy in patients with esophageal cancer; a higher dose, when applied conventional radiotherapy techniques, increases toxicities without improving survival. We investigated whether a high dose of 59.4 Gy using intensity-modulated radiotherapy (IMRT) improve survival without increasing toxicities. METHODS AND MATERIALS: Patients with inoperable thoracic esophageal squamous cell carcinoma (SCC) referred for dCRT were randomly assigned (1:1) to high-dose (HD) IMRT (59.4 Gy) or standard-dose (SD) IMRT (50.4 Gy). Chemotherapy consisted of six cycles of concurrent weekly paclitaxel and carboplatin, and a maximum of 2 cycles of consolidation chemotherapy. Nutritional intervention was implemented for patients with malnutrition on the basis of nutritional screening. The primary endpoint was median overall survival (mOS). Analyses were by modified intention to treat. RESULTS: Between April 30, 2016 and April 30, 2019, 167 patients were enrolled at nine participating centers in China, 71 patients in the HD and 73 patients in the SD groups were included in the analysis. 86.8% of the patients completed radiotherapy and 70.1% received five or six cycles of concurrent chemotherapy. The median follow-up was 36.0 months. The mOS was 28.1 months and 26.0 months in the HD and SD arms, respectively (P = .54). A total of seven treatment related deaths were observed. Grade 3 or worse treatment related toxicities were observed in 62% and 68.5% of the patients in the HD and SD arms, respectively (P = .675). CONCLUSIONS: For patients with inoperable thoracic esophageal SCC, a dose of 59.4 Gy did not improve survival compared with the standard dose of dCRT using IMRT.

17.
Front Psychol ; 13: 903250, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36405196

RESUMO

Background: Mental stress and imbalance of its two neural stress systems, the autonomic nervous system (ANS) and the hypothalamic-pituitary-adrenal (HPA) axis, are associated with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) and erectile dysfunction (ED). However, the comprehensive analyses of psychological stress and stress systems are under-investigated, particularly in CP/CPPS patients complicated by lower urinary tract symptoms (LUTS) and ED. Materials and methods: Participants were 95 patients in CP/CPPS+ED group, 290 patients in CP/CPPS group, 124 patients in ED group and 52 healthy men in control group. The National Institutes of Health Chronic Prostatitis Symptom Index (NIH-CPSI), the International Index of Erectile Function-5 (IIEF-5) and the International Prostate Symptom Score (IPSS) were used for assessing the disease severity of CP/CPPS, LUTS and ED. Psychometric self-report questionnaires including the Beck Anxiety Inventory (BAI), Perceived Stress Scale (PSS), Type A Personality Test (TAPT) and Symptom Checklist 90 (SCL-90) were completed for distress from physical symptoms. Twenty-five subjects per group were randomly selected for further investigating the changes of the HPA axis and ANS. Saliva samples were taken on 3 consecutive days at 8 specific times with strict reference to time of morning awakening for evaluation of free cortisol. Heart rate variability (HRV) as marker of the ANS was measured using 24 h electrocardiography, and time-and frequency-domain variables were analyzed. Results: The BAI and SCL-90 scores were significantly higher in the CP/CPPS+ED, CP/CPPS and ED groups compared with the control group (p < 0.01). The PSS scores of both groups with ED were significantly higher than the control group (p < 0.01). Compared with the CP/CPPS group, the differences of PSS, SCL-90 and TAPT scores were statistically significant in CP/CPPS+ED patients (p < 0.01). The IPSS scores were shown to have significantly positive correlations with BAI (r = 0.32, p < 0.0001), PSS (r = 0.18, p < 0.01) and SCL-90 (r = 0.19, p < 0.01) in the CP/CPPS patients. However, in all subjects, the IIEF-5 scores were shown to have significantly negative correlations with BAI (r = -0.17,p < 0.001), PSS (r = -0.25,p < 0.0001), SCL-90 (r = -0.20,p < 0.001) and quality of life score in NIH-CPSI (r = -0.14,p = 0.0075). Cortisol awakening response (CAR) parameters and diurnal cortisol levels did not significantly vary between the four groups. Time-dependent parameters of HRV also did not differ significantly across groups. In the frequency domain analysis, low frequency (LF) was significantly lower in ED patients when compared with CP/CPPS+ED patients (p = 0.044) and healthy controls (p = 0.005), high frequency (HF) power was significantly higher in healthy controls compared to patients with ED (p < 0.001), CP/CPPS (p < 0.001) and CP/CPPS+ED (p < 0.001), and the CP/CPPS+ED group had significantly higher LF/HF ratio than the control group (p = 0.001). Conclusion: CP/CPPS and ED patients score exceedingly high on most psychosocial variables. The symptom scores of LUTS and ED positively correlate with the severity of psychological stress. Our findings also suggest that the ANS sympathovagal imbalance is associated with ED and LUTS in CP/CPPS, whereas HPA axis activity is not.

18.
Front Neurol ; 13: 1017608, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36408508

RESUMO

Objective: This study investigates the association between vestibular function and prognosis in patients with unilateral idiopathic sudden sensorineural hearing loss (UISSNHL). Design: A retrospective analysis of 64 patients with UISSNHL was performed. Pure tone audiometry and vestibular function tests for otoliths and semicircular canals were performed to assess the influence of vestibular functional status on the outcome of patients with UISSNHL. Results: Patients with abnormal cervical vestibular evoked myogenic potential (cVEMP) or ocular vestibular evoked myogenic potential (oVEMP) responded less favorably to treatment. In the ineffective group, cVEMP was normal in four patients (6.3%) and oVEMPs in three (4.7%). Meanwhile, cVEMP was abnormal in 32 patients (50.0%) and oVEMP in 33 (51.6%). Better hearing recovery occurred in those with normal cVEMP (33.76 ± 15.07 dB HL improvement) or oVEMP (32.55 ± 19.56 dB HL improvement), but this was not the case in those with normal caloric tests. Patients with abnormalities in both cVEMP and oVEMP were less responsive to treatment and had worse hearing recovery than those with normal results in only one of the two tests. Conclusion: Abnormal oVEMP and/or cVEMP results indicate poor auditory outcomes in patients with UISSNHL. Patients with impaired otolith organ function are likely to have a larger and more severe pathological change in their inner ear.

19.
Zhongguo Zhen Jiu ; 42(11): 1211-5, 2022 Nov 12.
Artigo em Chinês | MEDLINE | ID: mdl-36397216

RESUMO

OBJECTIVE: To observe the effect of Huayu Tongluo (resolving stasis and promoting collateral circulation) moxibustion combined with intradermal needling on depressive symptoms, quality of life and cognitive impairment in patients with mild to moderate depression after cerebral infarction on the basis of western medicine treatment. METHODS: Fifty patients with mild to moderate depression after cerebral infarction were randomly divided into an acupuncture combined with western medication group (group A, 25 cases) and a western medication group (group B, 25 cases). In the group B, paroxetine hydrochloride tablets were taken orally, 20 mg after breakfast, once a day, and the dose could be adjusted to the maximum 40 mg/d according to the patients' condition, for 4 weeks totally. On the basis of the treatment in the group B, the group A was treated with Huayu Tongluo moxibustion, namely aconite cake-separated moxibustion at Baihui (GV 20) and suspended moxibustion at Dazhui (GV 14) and Shenting (GV 24), combined with intradermal needling at Shenmen (HT 7), Jianshi (PC 5), Zusanli (ST 36), etc. Huayu Tongluo moxibustion was performed 6 times a week, and intradermal needling was performed 3 times a week,for 4 weeks totally. In the two groups, the scores of Hamilton depression scale (HAMD), stroke specific quality of life scale (SS-QOL) and mini mental state examination (MMSE) were observed before and after treatment, and the clinical efficacy and safety were compared. RESULTS: After treatment, the HAMD score in the each group was decreased compared with that before treatment (P<0.05), and that in the group A was lower than the group B (P<0.05); after treatment, the SS-QOL score in the group A and MMSE score in the two groups were increased compared with those before treatment (P<0.05), and the SS-QOL score in the group A was higher than the group B (P<0.05). The total effective rate was 88.0% (22/25) in the group A, which was higher than 60.0% (15/25) in the group B (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (4.0% [1/25] vs 16.0%[4/25], P>0.05). CONCLUSION: On the basis of the treatment of western medication paroxetine hydrochloride tablets, Huayu Tongluo moxibustion combined with intradermal needling therapy can effectively improve the depressive symptoms, quality of life and cognitive impairment of patients with mild to moderate depression after cerebral infarction.


Assuntos
Moxibustão , Humanos , Qualidade de Vida , Paroxetina , Depressão/etiologia , Depressão/terapia , Infarto Cerebral/complicações , Infarto Cerebral/terapia
20.
Sci Adv ; 8(45): eabq2321, 2022 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-36367940

RESUMO

Solution-processable all-inorganic CsPbI3-xBrx perovskite holds great potential for pure red light-emitting diodes. However, the widely existing defects in this mixed halide perovskite markedly limit the efficiency and stability of present light-emitting diode devices. We here identify that intragrain Ruddlesden-Popper planar defects are primary forms of such defects in the CsPbI3-xBrx thin film owing to the lattice strain caused by inhomogeneous halogen ion distribution. To eliminate these defects, we develop a stepwise metastable phase crystallization strategy to minimize the CsPbI3-xBrx perovskite lattice strain, which brings planar defect-free CsPbI3-xBrx thin film with improved radiative recombination, narrowed emission band, and enhanced spectral stability. Using these high-quality thin films, we fabricate spectrally stable pure red perovskite light-emitting diodes, showing 17.8% external quantum efficiency and 9000 candela meter-2 brightness with color coordinates required by Rec. 2020.

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