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1.
J Affect Disord ; 260: 557-568, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31539693

RESUMO

OBJECTIVE: This study mapped the topological configuration of the default mode network (DMN) in patients with depressive symptoms after acute ischemic stroke. METHODS: The study sample comprised 63 patients: 36 with poststroke depressive symptoms (PSD) and 37 without PSD matched according to age, gender and the severity of stroke. PSD was defined by a cutoff of ≥ 7 on the 15-item Geriatric Depression Scale (GDS). Resting-state functional magnetic resonance imaging (fMRI) was used to examine functional connectivity (FC) to reconstruct the DMN. Network based statistics estimated the FC differences of the DMN between the PSD and non-PSD groups. Graph theoretical approaches were used to characterize the topological properties of this network. RESULTS: The study sample mainly comprised patients with mild to moderate stroke. A widespread hyper-connected configuration of the functional DMN was characterized in PSD group. The orbital frontal, dorsolateral prefrontal, dorsal medial prefrontal and, ventromedial prefrontal corticis, the middle temporal gyrus and the inferior parietal lobule were the functional hubs related to PSD. The nodal topology in inferior parietal lobule and superior frontal gyrus, overlapping with dorsal medial prefrontal and, ventromedial prefrontal cortices, tended to be functionally integrated in patients with PSD. After False Discovery Rate correction, no significant difference between the PSD and non-PSD groups was found with respect to the global and nodal metrics of the DMN. However, the correlations between these altered network metrics and severity of PSD were lacking. LIMITATIONS: The diagnosis of PSD was based on the GDS score rather than established with a structured clinical interview. CONCLUSIONS: The DMN in PSD was functionally integrated and more specialized in some core hubs such as the inferior parietal lobule and dorsal prefrontal cortex. The configuration of the subnetwork like DMN may be more essential in the pathogenesis of PSD than single stroke lesions.

2.
Chemosphere ; 238: 124645, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31472352

RESUMO

Bromophenols (BPs) are important organic compounds which have become dominant pollutants during these years. Our present study investigated the potential inhibition behaviour of BPs on the activity of one of the most important phase II drug-metabolizing enzymes (DMEs), UDP-glucuronosyltransferases (UGTs). Recombinant UDP-glucuronosyltransferases (UGTs)-catalyzed glucuronidation of 4-methylumbelliferone (4-MU) was utilized as the probe reaction. 100 µM of BPs was utilized as the inhibition screening concentrations, and the complete inhibition profile of UGT isoforms by BPs was obtained. UGT1A7 was the most vulnerable UGT isoform towards BPs. Some structure-activity relationship for the inhibition of UGTs by BPs was found, and this relationship can be furtherly explained by the hydrophobic contacts of BPs with the activity cavity of UGTs using in silico docking method. The inhibition kinetics determination showed that the inhibition kinetic parameter Ki value was calculated to be 2.85, 3.99 and 31.00 µM for the inhibition of UGT1A3, UGT1A7, and UGT2B7 by representative BPs, 2,4,6-TBP. Combined with in vivo exposure concentration of 2,4,6-TBP, in vitro-in vivo extrapolation (IVIVE) was employed to demonstrate the moderate possibility for the inhibition of UGT1A3 and UGT1A7 by 2,4,6-TBP. In conclusion, our study gave the full description towards the inhibition of BPs towards UGT isoforms, which will provide a new perspective for elucidating the toxicity mechanism of bromophenols (BPs).

3.
Food Chem ; 309: 125692, 2019 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-31670119

RESUMO

Persimmon tannin (PT) exhibits antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) isolated from retail pork. The involved molecular mechanisms were investigated for the first time using transcriptome and metabolome in this study. Results showed that subinhibitory concentration of PT (0.5 mg/ml) induced significant changes in MRSA at both transcriptional and metabolic levels, as 370 genes and 19 metabolites were differentially expressed. Bioinformatic analysis revealed that the varying genes and metabolites were mainly involved in pathways of membrane transport, amino acids, carbohydrate, and energy metabolism. The highlighted changes were those related to osmotic regulation, intracellular pH regulation, amino acid synthesis and metabolism, glycolysis, TCA cycle and iron metabolism, suggesting the multifaceted effects including cell membrane damage, amino acids limitation, energy metabolism disorder and iron deprivation induced by PT. The results provided insight into the anti-MRSA mechanism of PT, which is useful for PT's development and application in food safety.

4.
Sci Rep ; 9(1): 15756, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31673142

RESUMO

Gelsemium elegans is a flowering plant in the Loganiaceae. Because it can promote the growth of pigs and sheep, it is widely used, including in veterinary clinics, but little information is available about its biological effects. Here, we used high-throughput sequencing to characterize the differentially expressed genes (DEGs) in the ileums of pigs between a control group and a group fed Gelsemium elegans for 45 days. We found that Gelsemium elegans affected many inflammatory and immune pathways, including biological processes such as defense responses, inflammation and immune responses. Moreover, this study identified several important genes related to the anti-inflammatory activity of Gelsemium elegans (e.g., CXCL-8, IL1A, and CSF2), which will be beneficial for further study of the pharmacological mechanisms and clinical applications of Gelsemium elegans.

5.
Mar Drugs ; 17(11)2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31698828

RESUMO

Agar oligosaccharide (AOS) is a marine prebiotic with apparent anti-inflammatory, antioxidant and anti-tumor effects. During this study, different doses of AOS are added to a basal diet to evaluate its effects on the lifespan, motor vigor and reproduction of male Drosophila melanogaster. Additionally, the activities of Cu,Zn-superoxide dismutase (Cu,Zn-SOD) and catalase (CAT) and the malondialdehyde (MDA) content in male Drosophila are examined on the 10th, 25th and 40th days. The fly midguts are removed on the 10th and 40th days for analyses of the intestinal microbial community by 16S rDNA sequencing and the expression level of intestinal immunity genes by quantitative real-time PCR (RT-PCR). The results show that AOS significantly prolonged the average and maximum lifespan and increased the antioxidant capacity of male Drosophila. Additionally, AOS significantly regulated the structure of the intestinal flora of "old" flies (40 days) and upregulated the expression of immune deficiency (IMD) genes to improve the intestinal immunity, which could be beneficial for delaying aging in old flies. The above-described results provide a theoretical basis for the application of AOS, a type of marine oligosaccharide, as a nutritional supplement or immunomodulator.

6.
Macromol Rapid Commun ; : e1900394, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31702099

RESUMO

Diketopyrrolopyrrole (DPP)-based copolymers have received considerable attention as promising semiconducting materials for high-performance organic thin-film transistors (OTFTs). However, these polymers typically exhibit p-type or ambipolar charge-transporting characteristics in OTFTs due to their high-lying highest occupied molecular orbital (HOMO) energy levels. In this work, a new series of DPP-based n-type polymers have been developed by incorporating fused bithiophene imide oligomers (BTIn) into DPP polymers. The resulting copolymers BTIn-DPP show narrow band gaps as low as 1.27 eV and gradually down-shifted frontier molecular orbital energy levels upon the increment of imide group number. Benefiting from the coplanar backbone conformation, well-delocalized π-system, and favorable polymer chain packing, the optimal polymer in the series shows promising n-type charge transport with an electron mobility up to 0.48 cm2 V-1 s-1 in OTFTs, which is among the highest values for the DPP-based n-type polymers reported to date. The results demonstrate that incorporating fused bithiophene imide oligomers into polymers can serve as a promising strategy for constructing high-performance n-type polymeric semiconductors.

7.
Phys Rev Lett ; 123(14): 146801, 2019 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-31702216

RESUMO

We elucidate the nature of neutral collective excitations of fractional quantum Hall liquids in the long-wavelength limit. We demonstrate that they are chiral gravitons carrying angular momentum -2, which are quanta of quantum motion of an internal metric, and show up as resonance peaks in the system's response to what is the fractional Hall analog of gravitational waves. The relation with existing and possible future experimental work that can detect these fractional quantum Hall gravitons and reveal their chirality is discussed.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31707615

RESUMO

A series of MnCoOx flower-like hollow microspheres with various molecular proportions of reactant were prepared through simple solvothermal method for the ammonia selective catalytic reduction (SCR) at low temperatures. The as-prepared samples have been applied by various characterization techniques to explore the formation process of the morphology and physicochemical properties. The Mn(1)Co(1)Ox presented the optimal intrinsic catalytic performance (95% NOx conversion at 75 °C), favorable thermal stability, and strong SO2 resistance. The excellent properties mainly related to its higher specific surface area and abundant active sites originated from hollow microsphere special structure consists of abundant nanosheets, robust redox properties beneficial for the strong interaction between the manganese and cobalt, larger number of acidic sites and stronger acid strength, etc., which collaboratively dominate its catalytic properties of NH3-SCR at low temperatures.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31741219

RESUMO

As a key part of Bohai New Area development, Haixing County has been undergoing rapid development. In order to estimate potential risks of chemical parameters to human health of local residents, carcinogenic and non-carcinogenic risks via direct ingestion of drinking water were calculated using human health risk assessment (HHRS) based on triangular fuzzy number. The levels of pH, total dissolved solids, total harness, SO42-, Na+, Cl-, SO42-, F-, Fe (total iron), NO3-, and NO2- were more or less higher than the permissible limits except parameters As and Mn. The analysis results show that risk level for different crowds in the study area demonstrated an obvious variation, generally in the order of infants > children > adult males > adult females for non-carcinogenic risk values (Rn), while the sequence of the carcinogenic risk values (Rc) are adult males > adult females > children > infants. When the confidence level was 0.8, the non-carcinogenic risk values (Rn) through drinking water intake were higher than 1, and this implied that potential health impacts on human health for local residents. However, the risks of carcinogenic risk values (Rc) were lower than 1.0E-4, demonstrating minimal and acceptable health risk. Furthermore, according to the middle values (α = 1) of Rn, the total non-carcinogenic risks for local residents were obtained in the following order: GW (Gaowan Town) > XJ (Xinji-Xiangfang County) > ZM (Zhaomaotao County) > HX (Haixing-Suji Town) > ZH (Zhanghuiting County) > XS (Xiaoshan County), and ZM > XJ > GW > HX > XS > ZH for Rc. It was also found that the spatial distribution of fluoride level in drinking water is urgently needed to be identified. In conclusion, the potential health risks to residents should cause enough attention both from society and the academic community.

11.
Peptides ; : 170212, 2019 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-31770577

RESUMO

In type 2 diabetes, lipotoxicity damages islet microvascular endothelial cells (IMECs), leading to pancreatic islet ß cell dysfunction directly or indirectly. Glucagon-like peptide-1 (GLP-1) and its analogs have beneficial roles in endothelial cells. However, the protective effects of GLP-1 agents on IMECs and their potential mechanism remained obscure. In this study, exposure of MS-1 (a cell line derived from mouse IMECs) to different concentrations of palmitic acid (PA) was used to establish an injury model. The cells exposed to PA (0.25 mmol/L) were treated with a GLP-1 analog liraglutide (3, 10, 30, and 100 nmol/L). Reactive oxygen species (ROS) generation, apoptosis-related protein level, and endothelin-1 production were detected. The protein levels of signaling molecules were analyzed and specific inhibitors or blockers were used to identify involvement of signaling pathways in the effects of liraglutide. Results showed that PA significantly increased ROS generation and the levels of pro-apoptotic protein Bax, and decreased the levels of anti-apoptotic protein Bcl-2 and the mRNA expression and secretion of endothelin-1. Meanwhile, PA downregulated the protein levels of GLP-1 receptor (GLP-1R), phosphorylated protein kinase A (PKA), guanosine 5'-triphosphate cyclohydrolase 1 (GTPCH1), and endothelial nitric oxide synthase (eNOS). Furthermore, liraglutide ameliorated all these effects of PA in a dose-dependent manner. Importantly, GLP-1R antagonist exendin (9-39), PKA inhibitor H89, GTPCH1 inhibitor 2,4-diamino-6-hydroxypyrimidine, or NOS inhibitor N-nitro-L-arginine-methyl ester abolished the liraglutide-mediated amelioration in PA-impaired MS-1 cells. In conclusion, liraglutide ameliorates the PA-induced oxidative stress, apoptosis, and endothelin-1 secretion dysfunction in mouse IMECs through GLP-1R/PKA and GTPCH1/eNOS signaling pathways.

12.
Environ Pollut ; 255(Pt 2): 113253, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31627172

RESUMO

Metals are released from biochar (BC) in either the free or dissolved organic carbon (DOC)-combined form. The complexation of metals with DOC influences their toxicity and bioavailability in the environment. The endogenous release of metal species with heterogeneous DOC from BC is very complex; this process has been neglected and remains unaddressed in the literature to date. In this study, the yield and chemical properties of labile DOC from BC were characterized, and the release of endogenous metal/metalloid elements (K, Mg, Mn, Fe, Al, Cu, and Si) and their species from BC with various pyrolysis temperatures and particle sizes were systematically investigated under various solution chemistries. The results showed that pyrolysis temperature of BC significantly influenced the yield and composition of DOC and DOC-metal/metalloid complexes, while particle size had lower impact. The yield of BC-derived DOC significantly decreased and the components gradually changed from low-molecular weight and low-aromaticity hydrophilic humic acid-like substances to complex high-molecular weight and high-aromaticity hydrophobic substances as pyrolysis temperature increased from 200 to 700 °C. The release of total dissolved metals decreased with increasing pyrolysis temperature, while the highest total dissolved Si was released from BC with the moderate pyrolysis temperature (500 °C). The metal elements were mainly released in the DOC-combined form, while the released Si was mainly in the free form in the neutral water environment. The release of DOC increased while that of dissolved metals decreased with increasing solution pH. The release of total dissolved metals/metalloids increased but the ratio of the DOC-combined metals/metalloids decreased with increasing solution ionic strength. These results provide new insight into the understanding of endogenous metal/metalloid release from BC in the natural environment.

13.
Chem Res Toxicol ; 32(10): 2118-2124, 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31565933

RESUMO

N3-Methyl-2'-deoxyadenosine (MdA) is the major dA methylation product in duplex DNA. MdA blocks DNA replication and undergoes depurination at significantly higher rates than the native nucleotide from which it is derived. Recent reports on the effects of the nucleosome core particle (NCP) environment on the reactivity of N7-methyl-2'-deoxyguanosine (MdG) inspired this investigation concerning the reactivity of MdA in NCPs. NCPs containing MdA at selected positions were produced using a strategy in which the minor groove binding Me-Lex molecule serves as a sequence specific methylating agent. Hydrolysis of the glycosidic bond in MdA to form abasic sites (AP) is suppressed in a NCP. Experiments using histone variants indicate that the proximal, highly basic N-terminal tails are partially responsible for the decreased depurination rate constant. MdA also forms cross-links with histone proteins. The levels of MdA-histone DNA-protein cross-links (DPCMdA) decrease significantly over time and are replaced by those involving AP. The time dependent decrease in DPCMdA is attributed to the reversibility of their formation and the relatively rapid rate of AP formation from MdA. Overall, MdA reactivity in NCPs qualitatively resembles that of MdG.

14.
Sci Rep ; 9(1): 15131, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31641156

RESUMO

The debate on whether the nonlinear sorption of nonionic organic compounds (NOCs) by soil organic matter (SOM) is captured by nonlinear partition or adsorption has been going on for decades because the used SOM samples are complex mixtures from various precursors with varied humification degrees in natural environment. Therefore, in this study, hydrothermal method was employed to prepare humus-like substances from a sole precursor (i.e., rice straw) with various humification degrees for nonlinear sorption of 25 aromatic compounds, then to have an insight into the underlying mechanisms of the nonlinear sorption of NOCs by SOM. It was observed that the increasing humification degree of humus-like substances, i.e., decreasing in the polarity ((O + N)/C) and increasing in the aromaticity, result in the increase of isotherm nonlinearity and sorption capacity/affinity of NOCs. Sorption capacity of NOCs, obtained by isotherm fitting using Dubinin-Astakhov (DA) model and Dual-Mode (DM) model, are positively correlated with their solubility in water and octanol, indicating the nonlinear sorption could be captured by nonlinear partition mechanism. Specific interactions including hydrogen-bonding interaction and π-π interaction between aromatic structures of humus-like substances and organic molecules could be responsible for the nonlinear partition and the increase of sorption affinity with the enhancement of humification degree. These obtained correlations are valuable for understanding the underlying mechanisms of nonlinear sorption and elucidating the transport of NOCs in the environment.

15.
World Neurosurg ; 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31629151

RESUMO

OBJECTIVE: Primary decompressive craniectomy (DC) is an important therapeutic technique for severe head-injured patients with space-occupying lesions in emergency situations, but these patients are still at high risk for unfavorable outcomes. This study aimed to investigate the predictors of 30-day mortality in adult patients undergoing primary DC after traumatic brain injury (TBI). METHODS: All adult patients (≥18 years of age) who underwent primary DC from January 2012 to March 2019 were included. Demographic, clinical, surgical, and laboratory variables were collected for analysis. Early mortality was defined as 30-day mortality after DC. First, a univariate analysis (P < 0.05) was used to compare survivors and nonsurvivors. Multivariate logistic regression analysis was used to identify the predictors of 30-day mortality for patients who underwent primary DC. RESULTS: A total of 387 patients were enrolled in the study. The 30-day mortality was 31.52% (122/387). The median age at presentation was 49 years (interquartile range, 38-60), and 316 (81.65%) patients were male. In the multivariate logistic regression analysis, the factors associated with 30-day mortality included age (odds ratio [OR], 1.068; 95% confidence interval [CI], 1.040-1.096; P < 0.001), bilateral unreactive pupils (OR, 12.734; 95% CI, 4.129-39.270; P < 0.001), subdural hemorrhage (OR, 3.468; 95% CI, 1.305-9.218; P < 0.013), completely effaced basal cistern (OR, 3.52; 95% CI, 1.568-7.901; P = 0.002), intraoperative hypotension (OR, 11.532; 95% CI, 4.222-31.499; P < 0.001), preoperative activated partial thromboplastin time (OR, 6.905; 95% CI, 2.055-23.202; P = 0.002), and Injury Severity Score (OR, 1.081; 95% CI, 1.031-1.133; P = 0.002). CONCLUSIONS: In patients undergoing primary DC after traumatic brain injury, the predictors of 30-day mortality include age, bilateral unreactive pupils, subdural hemorrhage, completely effaced basal cistern, intraoperative hypotension, preoperative activated partial thromboplastin time, and Injury Severity Score.

16.
Brain Stimul ; 2019 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-31606448

RESUMO

BACKGROUND: Transcranial direct current stimulation (tDCS) has been explored in epilepsy with limited samples, varied parameters, and inconclusive results. We aimed to study the efficacy of tDCS for patients with refractory focal epilepsy. METHOD: We conducted a randomized, double-blind, sham-controlled, and three-arm (Group 1 (sham), Group 2 (20-min), and Group 3 (2 × 20-min)) tDCS parallel multicenter study. The primary outcome measurement was seizure frequencies (SFs). The study consisted of 28-days baseline, 14-days treatment, and 56-days follow-up. The cathode was placed over the epileptogenic focus, and the current intensity was 2 mA. The generalized estimating equations model, one-way analysis of variance, chi-square and Kruskal-Wallis test were used for analysis. RESULTS: Of the 82 enrolled patients, 70 patients were included for final analysis (Group 1, n = 21; Group 2, n = 24; and Group 3, n = 25). There was a significant reduction in SFs for both active tDCS groups compared with the sham group. Patients in Group 2 showed a significantly 50.73-21.91% greater reduction in SFs that lasted for 4 weeks (p = 0.008-0.060). Patients in Group 3 showed a significantly 63.19-49.79% greater reduction in SFs compared with the sham group that lasted for 5 weeks (p = 0.011-0.045). Patients in Group 3 had a 64.98-66.32% greater reduction in SFs at W9-W10, when compared with Group 2 (p = 0.021-0.022). CONCLUSION: Fourteen consecutive days tDCS significantly decreased SFs in patients with refractory focal epilepsy, with 2 × 20-min daily stimulation protocol being superior to 20-min daily stimulation protocol.

17.
Biomaterials ; 225: 119539, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31622821

RESUMO

Photostimulation has been widely used in neuromodulation. However, existing optogenetics techniques require genetic alternation of the targeted cell or tissue. Here, we report that neural stem cells (NSCs) constitutionally express blue/red light-sensitive photoreceptors. The proliferation and regulation of NSCs to neuronal or glial cells are wavelength-specific. Our results showed a 4.3-fold increase in proliferation and 2.7-fold increase in astrocyte differentiation for cells under low-power blue monochromatic light exposure (455 nm, 300 µW/cm2). The melanopsin (Opn4)/transient receptor potential channel 6 (TRPC6) non-visual opsin serves as a key photoreceptor response to blue light irradiation. Two-dimensional gel electrophoresis coupled with mass spectrometry further highlighted the Jun activation domain-binding protein 1 (Jab1) as a novel and specific modulator in phototransduction pathways induced by blue light exposure. Quiescent adult NSCs reside in specific regions of the mammalian brain. Therefore, we showed that melanopsin/TRPC6 expressed in these regions and blue light stimulation through optical fibers could directly stimulate the NSCs in vivo. Upconversion nanoparticles (UCNPs) converted deep-penetrating near-infrared (NIR) light into specific wavelengths of visible light. Accordingly, we demonstrated that UCNP-mediated NIR light could be used to modulate in vivo NSC differentiation in a less invasive manner. In the future, this light-triggered system of NSCs will enable nongenetic and noninvasive neuromodulation with therapeutic potential for central nervous system diseases.

18.
World J Surg ; 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31637507

RESUMO

BACKGROUND: The prognostic significance of preoperative plasma fibrinogen in patients with operable gastric cancer remains under debate. This study aimed to elucidate the prognostic value of fibrinogen in gastric cancer patients underwent gastrectomy. METHODS: A total of 4351 patients with gastric cancer collected from three comprehensive medical centers were retrospectively evaluated. Patients were categorized by minimum P value using X-tile, while the baseline confounders for fibrinogen was balanced through propensity score matching (PSM). The relationships between fibrinogen and other clinicopathologic features were evaluated, and nomogram was constructed to assess its prognostic improvement compared with TNM staging system. RESULTS: Fibrinogen was significantly correlated with macroscopic type, tumor differentiation, tumor size, and T and N stage. The factors, fibrinogen and T stage as well as N stage, were identified to be independent prognostic factors after PSM. Nomogram based on fibrinogen demonstrated a smaller Akaike information criterion (AIC) and a larger concordance index (C-index) than TNM staging system, illustrating that fibrinogen might be able to improve the prognostic accuracy. CONCLUSIONS: Preoperative plasma fibrinogen levels in gastric cancer patients were significantly correlated with tumor progression, which could be regarded as a reliable marker for survival prognostic prediction.

19.
Medicine (Baltimore) ; 98(39): e17245, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31574838

RESUMO

RATIONALE: Over the past decade, although several new entities of renal tumors have emerged, a form of renal cell carcinoma (RCC) that morphologically resembles epithelial-myoepithelial carcinoma has not been reported thus far. Herein, we describe a case of an unusual renal tumor that remained unclassified under a current RCC subtype, and briefly present its morphologic, immunophenotypic, and genetic features. PATIENT CONCERNS: The patient was an 85-year-old man who presented with hematuria and flank pain. Imaging studies revealed a left renal mass without enlarged lymph nodes. There were no abnormal masses or nodules in other organs. DIAGNOSES: The patient underwent no other treatment except the left radical nephrectomy under a clinical diagnosis of invasive urothelial carcinoma and was discharged on the thirteenth day. Histologically, the renal tumor showed biphasic proliferation of epithelial (strongly cytokeratin-positive; P63, P40, and vimentin-negative) and myoepithelial (strongly vimentin-positive; focal P63 and P40-positive; and weakly cytokeratin-positive) cells arranged in a perivascular pseudorosette-like pattern. No mutations were detected in multiple gene tests. According to the pathological structure, the patient was diagnosed as primary epithelial myoepithelial carcinoma-like renal tumor. INTERVENTIONS: To the best of our knowledge, the present tumor has not been previously described, and thus, this variant has not been integrated into a known form of PCC. Therefore, we cannot diagnose this type of tumor with other types of kidney tumors. OUTCOMES: Three years after primary diagnosis, the patient died of multiple organ failure result from multiple distant metastases. LESSONS: We present the first case of carcinoma of the kidney with EMC-like features and a perivascular pseudorosette-like growth pattern. Clinicians should be aware of the features of this uncommon variant of RCC to avoid diagnostic delays or misdiagnosis and prevent unnecessary or inappropriate treatment.


Assuntos
Carcinoma de Células Renais/patologia , Neoplasias Renais/patologia , Mioepitelioma/patologia , Idoso de 80 Anos ou mais , Carcinoma de Células Renais/genética , Evolução Fatal , Humanos , Imunofenotipagem , Rim/patologia , Neoplasias Renais/genética , Masculino , Mioepitelioma/genética , Formação de Roseta
20.
Aquat Toxicol ; 216: 105323, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31606665

RESUMO

Graphene oxide (GO), used in a wide variety of applications, is increasingly being introduced into aquatic environments; this situation calls for research on GO toxicity to assess its environmental risks. In this study, the toxic effect of GO to E.coli was studied before and after its aggregation equilibrium in the synthetic surface waters (the soft water, moderately hard water, and hard water) to reveal the effects of GO aggregation and solution hardness. The cytotoxicity of GO increased with increasing solution hardness while decreased after GO aggregation. The 3 h 50% inhibitory concentration (IC50) values of dispersed GO in the soft water, moderately hard water, and hard water were 12.2 ±â€¯2.2, 8.5 ±â€¯1.5, and 4.0 ±â€¯1.0 mg/L, respectively. After 24 h shaking (aggregation equilibrium) in the synthetic surface waters, the dispersed GO aggregated and the 3 h IC50 values of GO aggregates in the three synthetic waters were 40.3 ±â€¯6.9, 15.9 ±â€¯ 2.2, and 7.5 ±â€¯1.5 mg/L, respectively. The dispersed GO sheets wrapped E. coli cells and cut the cell membrane, resulting in the disruption of cell membrane and the cell inactivation. With increasing water hardness, the heteroaggregation between GO sheets/aggregates and E. coli cells was enhanced, resulting in the increase of toxic effect. The GO aggregates could also entrap E.coli cells while exhibited limited effect on cell membrane disruption without sharp edges, thereby causing the lower toxic effect compared with the dispersed GO sheets. These outcomes shed new light on the assessment of ecological effects of GO.

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