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1.
Chemosphere ; 287(Pt 4): 132454, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34610376

RESUMO

This study aimed to clarify that long-term leguminous grass mulching (crown vetch (CV) and white clover (WC)) and gramineous grass (orchardgrass (OG)) drive the distribution of soil aggregates and are associated with dissolved organic matter (DOM) components. Excitation emission spectroscopy and parallel factor analysis (EEM-PARAFAC) were used to evaluate the influence of different grass mulches among aggregates. The results indicated that legumes had a more significant impact on the distribution of aggregates and DOM content than gramineae grass mulching. Leguminous grass mulching significantly increased the proportion of macroaggregates >250 µm (74.65%-83.50%) and aggregates associated with dissolved organic carbon (DOC), especially in microaggregates <250 µm (172.27 mg kg-1 to 391.55 mg kg-1). In addition, leguminous grass mulching (CV and WC) contributed more to the increase in soil total nitrogen (TN) and three identified fluorescent components (UVC humic-like, UVA humic-like and protein-like). The component of UVC humic-like relative abundance decreased (48.66%-36.57%), and the protein-like component increased (21.88%-36.50%) as the aggregate size decreased, but the DOM three compositions did not change. The DOM of macroaggregates had higher aromaticity and lower molecular weight than microaggregates, and the highest abundance of UVC humic-like component (54.52%) was found in the gramineous (OG) large macroaggregates, while the higher abundance of protein-like components (31.07%-36.50%) occurs in leguminous mulching (CV and WC) microaggregates. The results contribute to a further understanding of the dynamic process by which grass mulching mediates aggregate formation and DOM component transformation in semiarid apple orchards under grass waste management.


Assuntos
Carbono , Substâncias Húmicas , Análise Fatorial , Substâncias Húmicas/análise , Poaceae , Solo , Espectrometria de Fluorescência
2.
J Proteomics ; 250: 104385, 2022 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-34606990

RESUMO

Candida albicans is the most common human fungal pathogen in immunocompromised individuals. With the emergence of clinical fungal resistance, there is an urgent need to develop novel antifungal agents. AMP-17, a novel antimicrobial peptide from Musca domestica, has an antifungal effect against C. albicans, but its mechanism of antifungal action remains unclear. In the current study, we performed a proteomics analysis in C. albicans using TMT technique under the treatment of AMP-17. A total of 3931 proteins were identified, of which 3600 included quantitative information. With a 1.5-fold change threshold and a t-test p-value < 0.05 as standard, 423 differentially expressed proteins (DEPs) were up-regulated and 180 DEPs were down-regulated in the AMP-17/control. Notably, GO enrichment revealed that DEPs associated with the cell wall, RNA and oxidative stress were significantly up-regulated, while DEPs involved in ergosterol metabolism and membrane were significantly down-regulated in the AMP-17/control. KEGG pathway enrichment revealed that DEPs involved seven significant metabolic pathways, mainly involved oxidative phosphorylation, RNA degradation, propanoate metabolism and fatty acid metabolism. These results show that AMP-17 induces a complex organism response in C. albicans, indicating that AMP-17 may inhibit growth by affecting multiple targets in C. albicans cells. SIGNIFICANCE: Antimicrobial peptides (AMPs) are an important part of the innate immune system of organisms and having broad range of activity against fungi, bacteria and viruses. These AMPs are considered as probable candidate for forthcoming drugs, due to their broad range of activity, lesser toxicity and decreased resistance development by target cells. AMP-17, a novel antimicrobial peptide from M. domestica, has significant antifungal activity against C. albicans. It has been confirmed that AMP-17 can play an antifungal effect by destroying the cell wall and cell membrane of C. albicans in previous studies, but its mechanism of action at the protein level is currently unclear. In the current study, using the TMT-based quantitative proteomics method, 603 differentially expressed proteins were identified in the cells of C. albicans treated with AMP-17 for 12 h, and these DEPs were closely related to cell wall, cell membrane, RNA degradation and oxidative stress. The results provide new insights into the potential mechanism of action of AMP- 17 against C. albicans. Meanwhile, it provides certain technical support and theoretical basis for the research and development of novel peptide drugs.

3.
Artigo em Inglês | MEDLINE | ID: mdl-34735345

RESUMO

Identifying geometric features from sampled surfaces is a significant and fundamental task. The existing curvature-based methods that can identify ridge and valley features are generally sensitive to noise. Without requiring high-order differential operators, most statistics-based methods sacrifice certain extents of the feature descriptive powers in exchange for robustness. However, neither of these types of methods can treat the surface boundary features simultaneously. In this paper, we propose a novel neighbor reweighted local centroid (NRLC) computational algorithm to identify geometric features for point cloud models. It constructs a feature descriptor for the considered point via decomposing each of its neighboring vectors into two orthogonal directions. A neighboring vector starts from the considered point and ends with the corresponding neighbor. The decomposed neighboring vectors are then accumulated with different weights to generate the NRLC. With the defined NRLC, we design a probability set for each candidate feature point so that the convex, concave and surface boundary points can be recognized concurrently. In addition, we introduce a pair of feature operators, including assimilation and dissimilation, to further strengthen the identified geometric features. Finally, we test NRLC on a large body of point cloud models derived from different data sources. Several groups of the comparison experiments are conducted, and the results verify the validity and efficiency of our NRLC method.

4.
Chem Sci ; 12(39): 12971-12976, 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34745527

RESUMO

Copper-catalyzed electrochemical direct chalcogenations of o-carboranes was established at room temperature. Thereby, a series of cage C-sulfenylated and C-selenylated o-carboranes anchored with valuable functional groups was accessed with high levels of position- and chemo-selectivity control. The cupraelectrocatalysis provided efficient means to activate otherwise inert cage C-H bonds for the late-stage diversification of o-carboranes.

5.
World J Clin Cases ; 9(29): 8906-8914, 2021 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-34734074

RESUMO

BACKGROUND: Although acute pancreatitis associated with hyperparathyroidism has occasionally been reported, acute pancreatitis with metabolic encephalopathy caused by hyperparathyroidism combined with paraneoplastic syndrome is an extremely rare entity and poorly described in the literature. CASE SUMMARY: We present a case of a 56-year-old female with upper abdominal discomfort and intermittent nausea and vomiting for 1 wk, without apparent abdominal pain or bloating, no jaundice and decreased blood pressure at the outset. The patient was ultimately diagnosed with moderately severe acute pancreatitis (according to the revised Atlanta classification of acute pancreatitis) combined with metabolic encephalopathy secondary to hypercalcemia caused by primary hyperparathyroidism associated with paraneoplastic syndrome. After active treatment of acute pancreatitis, massive fluid resuscitation, resection of parathyroid and uterine malignant tumors, neoadjuvant chemotherapy and other treatments, her serum calcium eventually returned to the normal level. The patient was successfully discharged from hospital. CONCLUSION: This is the first case of acute pancreatitis caused by primary hyperparathyroidism associated with paraneoplastic syndrome.

6.
Clin Appl Thromb Hemost ; 27: 10760296211059498, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34755551

RESUMO

This study investigates the prognostic value of immune cell subsets in assessing the risk of death in patients with sepsis. This retrospective study collected 169 patients from March 2020 to February 2021 at our hospital. Baseline data were collected from patients. The absolute values (Abs) and percentages (%) of immune cell subsets for lymphocytes, T cells, CD4+ cells, CD8+, B cells, NK cells, and NKT cells were measured using flow Cytometry. Among the included patients, 43 patients were in the nonsurvivor group and 126 patients were in the survivor group. The age of patients in the nonsurvivor survivor was higher than that of survivor group patients (P = .020). SOFA, APACHE II, C-reactive protein, and procalcitonin were higher in the nonsurvivor group than in the survivor group (all P values < .05). Multivariate regression analysis showed that lymphocytes (%) and SOFA were independent risk factors affecting patients' prognosis. Lymphocytes (%) have the highest area under the receiver operating characteristic (ROC) curve (0.812). The model area under the ROC curve for immune cell subsets was 0.800, with a sensitivity of 72.09%, and specificity of 79.27% (z = 7.796, P < .001). Analysis of patient prognosis by immune cell subsets diagnostic showed statistically significant differences in the grouping of cut-off values for all 5 indicators (all P < .05). The lymphocytes (%) and SOFA score are independent risk factors affecting the prognosis of patients. A moderate predictive power for mortality in sepsis patients by immune cell subsets model.

7.
Org Biomol Chem ; 19(45): 9867-9871, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34734622

RESUMO

A [6 + 3] annulation reaction of Morita-Baylis-Hillman carbonates and dicyanoheptafulvene is accomplished by employing commercially available triphenylphosphine as the Lewis base catalyst. A spectrum of densely functionalized bicyclo[4.3.1]decane architectures are efficiently constructed with exclusive diastereoselectivity and good yield (up to 95%).

8.
Pharmazie ; 76(10): 494-498, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34620277

RESUMO

Background: After a stroke, more than 50% of patients are suffering from dysphagia. Because the swallowing dysfunction is closely related to some neural pathways, the probing of the neuro-molecular mechanism of dysphagia is very important for future diagnosis and treatment. Methods: Our study is a typical causal study with the purpose of exploring molecular mechanisms. In this study, a rat model of dysphagia after stroke was constructed, and ARID1B overexpression plasmid was injected into the rat body through tail vein injection. The number of swallows and the swallowing response time induced by distilled water in each group of rats on the 7 th and 14 th day after modeling were detected. After 14 days of successful model establishment, the rat brain tissues were collected, part of the brainstem nucleus tractus solitarius and nucleus suspicious tissues were analyzed with a Ca2+ fluorescent indicator to analyze the intracellular concentration of Ca2+. For a part of the brainstem nucleus tractus solitarius and suspected nucleus tissues, immunohistochemistry was used to analyze the expression characteristics of genes ARID1B and TACR1 related proteins. The cerebrospinal fluid of brain tissue was collected, and the expression of gene TAC1 related protein in cerebrospinal fluid was analyzed by ELISA. For a part of the brainstem nucleus tractus solitarius and suspicious nucleus tissues, western blot was used to analyze the expression of gene SMARCA1 related protein, protein UNC80 and NALCN. Results: The detection of swallowing characteristics and the detection of intracellular Ca2+ concentration indicate the serious impact of stroke on swallowing function. The protein expression showed a consistent trend, which also showed that the overexpression of gene ARID1B can improve swallowing function to a certain extent. Conclusion: Due to our experiments, the molecular mechanism related to dysphagia was explored to a certain extent. At the same time, we found that the overexpression of the gene ARID1B can improve the swallowing function.

9.
Open Life Sci ; 16(1): 1010-1021, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34632071

RESUMO

It has been shown that the activation of calcineurin is involved in regulating ion channel remodeling in hypertrophic cardiomyocytes. But the precise role of calcineurin in the regulation of transient outward potassium current (I to), an ion channel associated with fatal arrhythmia, remains controversial. This study aimed to examine the effects of calcineurin Aß (CnAß) gene knockdown on I to channel remodeling and action potential duration (APD) in the hypertrophic ventricular myocytes of neonatal rats. Results showed that phenylephrine stimulation caused hypertrophy of ventricular myocytes, upregulation of CnAß protein expression, downregulation of Kv4.2 mRNA and protein expression, a decrease in I to current density, and prolongation of APD. CnAß gene knockdown significantly inhibited the effects of phenylephrine stimulation. Our data indicate that CnAß gene knockdown can inhibit I to channel remodeling and APD prolongation in hypertrophic neonatal rat ventricular myocytes. This finding suggests that calcineurin may be a potential target for the prevention of malignant ventricular arrhythmia in a hypertrophic heart.

10.
Infect Drug Resist ; 14: 4191-4205, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34675564

RESUMO

Background: Few studies have studied the relationship between blood culture and mortality in sepsis patients. The aim of this study was to compare the characteristics and outcomes of positive and negative blood culture sepsis. Methods: We performed a study on 640 patients suffering from sepsis in Beijing Chao-Yang Hospital from October 2017 to December 2019. The primary findings revolved around length and expenditure of hospital stay, the possibility of suffering from acute respiratory distress syndrome (ARDS), and any requirements for mechanical ventilation. The secondary findings revolved around whether the patient died early (28-day) or late (28-to-90-day). Results: A total of 592 of the 640 patients met the inclusion criteria for sepsis, with 274 of them having culture-positive results. The culture-positive patients were mostly elderly suffering from diabetes and at risk of cancer, with a higher white blood cell count, and higher procalcitonin. Additionally, they scored higher in their acute physiology and chronic health evaluation II score (15 vs.11, P=0.010), as well as in their predisposition, infection, response, and organ dysfunction (17 vs 11, P<0.001) than the individuals in the culture-negative group. Culture-positive patients had a longer duration of hospital stay (14 vs 6, P<0.001) and higher in-hospital mortality (14.6% vs 8.5%, P=0.019) than culture-negative ones. No significant difference in intensive care unit (ICU) mortality (45.7% vs.36.4%, P=0.254) or early mortality (9.5% vs 7.2%, P=0.321) was noted between the two groups. However, the culture-positive patients had increased late mortality (15.7% vs.6.9%, P=0.001), when compared with those with culture-negative results in the cohort. Furthermore, the culture-positive patients who received the appropriate antibiotics early had a lower mortality rate than the culture-negative patients (7.3% vs.14.2%, P=0.008). Conclusion: Culture-positive patients had higher in-hospital mortality, comparable early mortality, and worse late mortality than the culture-negative patients. Early appropriate use of antibiotics might reduce mortality and improve clinical prognosis.

11.
Front Cardiovasc Med ; 8: 727546, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34692782

RESUMO

Background: The origin distribution in right ventricular outflow tract (RVOT) ventricular arrhythmias (VAs), as well as the initial ablation effectiveness of reversed U-curve method and antegrade method, remains unclear. Objectives: To investigate the origin distribution of RVOT-type VAs and compare the initial ablation effectiveness of the two methods. Method: Consecutive patients who had idiopathic RVOT-type VAs were prospectively enrolled. After activation mapping, patients were randomly assigned to supravalvular strategy using the reversed U-curve or subvalvular strategy using the antegrade method. The primary outcome was initial ablation (IA) success, defined as the successful ablation within the first three attempts. Results: Sixty-one patients were enrolled from November 2018 to June 2020. Activation mapping revealed that 34/61 (55.7%) of the earliest ventricular activating (EVA) sites were above the pulmonary valves (PVs). The IA success rate was 25/33 (75.8%) in the patients assigned to supravalvular strategy as compared with 16/28 (57.1%) in those assigned to subvalvular strategy (p = 0.172). Multivariate analysis revealed a substantial and qualitative interaction between the EVA sites and IA strategies (p interaction < 0.001). Either strategy had a remarkably higher IA success rate in treating its ipsilateral EVA sites than contralateral ones (p < 0.0083). Conclusion: Of the idiopathic RVOT-type VA origins, half were located above the PV. The supravalvular and subvalvular strategies did not differ in IA success rates. However, they were complementary to reveal the EVA sites and facilitate ipsilateral ablation, which produces a significantly higher IA success rate. Clinical Trial Registration: Chinese Clinical Trial Registry number, https://www.chictr.org.cn/showproj.aspx?proj=45623, ChiCTR2000029331.

12.
Biology (Basel) ; 10(10)2021 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-34681095

RESUMO

Freezing stress in winter is the biggest obstacle to the survival of C. sinensis in mid-latitude and high-latitude areas, which has a great impact on the yield, quality, and even life of C. sinensis every year. In this study, transcriptome and metabolome were used to clarify the freezing resistance mechanism of 60-year-old natural overwintering C. sinensis under freezing stress. Next, 3880 DEGs and 353 DAMs were obtained. The enrichment analysis showed that pathways of MAPK and ABA played a key role in the signal transduction of freezing stress, and Pyr/PYL-PP2C-SnRK2 in the ABA pathway promoted stomatal closure. Then, the water holding capacity and the freezing resistance of C. sinensis were improved. The pathway analysis showed that DEGs and DAMs were significantly enriched and up-regulated in the three-related pathways of phenylpropanoid biosynthesis, flavone and flavonol biosynthesis, and flavonoid biosynthesis. In addition, the carbohydrate and fatty acid synthesis pathways also had a significant enrichment, and the synthesis of these substances facilitated the freezing resistance. These results are of great significance to elucidate the freezing resistance mechanism and the freezing resistance breeding of C. sinensis.

13.
Plant Biotechnol J ; 2021 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-34626524

RESUMO

Genetically-modified crops expressing Bacillus thuringiensis (Bt) proteins have been widely cultivated, permitting an effective non-chemical control of major agricultural pests. While their establishment can enable an area-wide suppression of polyphagous herbivores, no information is available on the impact of Bt crop abandonment in entire landscape matrices. Here, we detail a resurgence of the cosmopolitan bollworm Helicoverpa armigera following a contraction of Bt cotton area in dynamic agro-landscapes over 2007-2019 in North China Plain. An 80% reduction in Bt cotton was mirrored in a 1.9-fold increase of ambient H. armigera population levels, culminating in 1.5-2.1-fold higher yield loss and a 2.0-4.4-fold increase in pesticide use frequency in non-Bt crops (i.e. maize, peanut, soybean). Our work unveils the fate of herbivorous insect populations following a progressive dis-use of insecticidal crop cultivars, and hints at how tactically deployed Bt crops could be paired with agro-ecological measures to mitigate the environmental footprint of crop production.

14.
Acta Biochim Biophys Sin (Shanghai) ; 53(11): 1538-1546, 2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34636395

RESUMO

BACE1 antisense RNA (BACE1-AS) is implicated in promoting cell proliferation in different types of tumors. However, the function and mechanism of BACE1-AS in hepatocellular carcinoma (HCC) are still unclear. In the present study, we found that the relative expression of BACE1-AS in HCC cell lines, HCC tissues, and serum samples of HCC patients was significantly increased, and its high expression was correlated with the poor prognosis of HCC patients. In addition, overexpression of BACE1 promoted HCC cell proliferation, cell cycle progression, migration, and invasion, but inhibited cell apoptosis, while knockdown of BACE1 exerted the opposite role. Furthermore, BACE1-AS sponged miR-214-3p and inhibited its expression, thus promoting Apelin (APLN) expression. Overexpression or knockdown of miR-214-3p could partially reverse the abnormal proliferation, cell cycle progression, migration, invasion, and apoptosis caused by overexpression or knockdown of BACE1. These findings suggest that the BACE1-AS/miR-214-3p/APLN axis is a novel signaling pathway that facilitates HCC.

15.
Front Genet ; 12: 704712, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34671381

RESUMO

Background: To explore the biological and clinical effects of titin-antisense RNA1 (TTN-AS1) in bladder cancer (BC) and the association between TTN-AS1 and activating transcription factor 2 (ATF2) in BC. Methods: The Kaplan-Meier method was performed to analyze the association between the expression of TTN-AS1 and prognosis of BC patients from TCGA data set and our institution. Quantitative real-time PCR (RT-PCR) was conducted to explore the expression of TTN-AS1 between the patients who underwent TURBT and Re-TURBT. MTT, colony formation, and tumor formation assays were conducted to evaluate the effect of TTN-AS1 on the ability of proliferation in BC cell lines. Transwell assay was performed to evaluate the effect of TTN-AS1 on the ability of invasion in BC cell lines. Bioinfomatics and immunohistochemical staining was used to identify the relationship between TTN-AS1 and ATF2. Results: The higher expression of TTN-AS1 was related to poorer disease-free survival (DFS) in patients with BC. The expression of TTN-AS1 was higher in BC patients who underwent Re-TURBT compared with BC patients who underwent TURBT. Knocking down TTN-AS1 resulted in inhibiting the ability of proliferation and invasion of BC cells. ATF2 may serve as a downstream target of TTN-AS1 in BC, and the high expression of ATF2 is also related to adverse DFS. Conclusion: Our study reveals that TTN-AS1 serves as an oncogene by activating ATF2 in BC. The findings suggest that TTN-AS1 may act as a novel therapeutic target for patients with BC.

16.
Front Plant Sci ; 12: 770556, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34675959

RESUMO

[This corrects the article DOI: 10.3389/fpls.2020.581513.].

17.
Cell Prolif ; : e13145, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34668606

RESUMO

OBJECTIVES: Success in pregnancy in mammals predominantly depends on a well-developed placenta. The differentiation of invasive trophoblasts is a fundamental process of placentation, the abnormalities of which are tightly associated with pregnancy disorders including preeclampsia (PE). Monoclonal nonspecific suppressor factor beta (MNSFß) is an immunosuppressive factor. Its conventional knockout in mice induced embryonic lethality, whereas the underlying mechanism of MNSFß in regulating placentation and pregnancy maintenance remains to be elucidated. METHODS: Trophoblast-specific knockout of MNSFß was generated using Cyp19-Cre mice. In situ hybridization (ISH), haematoxylin and eosin (HE), immunohistochemistry (IHC) and immunofluorescence (IF) were performed to examine the distribution of MNSFß and insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) at the foeto-maternal interface. The interaction and expression of MNSFß, IGF2BP2 and invasion-related molecules were detected by immunoprecipitation (IP), immunoblotting and quantitative real-time polymerase chain reaction (qRT-PCR). The cell invasion ability was measured by the Transwell insert assay. RESULTS: We found that deficiency of MNSFß in trophoblasts led to embryonic growth retardation by mid-gestation and subsequent foetal loss, primarily shown as apparently limited trophoblast invasion. In vitro experiments in human trophoblasts demonstrated that the conjugation of MNSFß with IGF2BP2 and thus the stabilization of IGF2BP2 essentially mediated the invasion-promoting effect of MNSFß. In the placentas from MNSFß-deficient mice and severe preeclamptic (PE) patients, downregulation of MNSFß was evidently associated with the repressed IGF2BP2 expression. CONCLUSIONS: The findings reveal the crucial role of MNSFß in governing the trophoblast invasion and therefore foetal development, and add novel hints to reveal the placental pathology of PE.

18.
Mater Horiz ; 8(12): 3394-3398, 2021 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-34676385

RESUMO

The nonlinear optical (NLO) efficiency (dij) and laser-induced damage threshold (LIDT) of a material are mainly determined by their covalency and ionicity, respectively, the incompatibility between which makes balancing the dij and LIDT challenging in an IR NLO material. The topological feature (fractal dimension) of the electron localization function (ELF) map (distribution of covalency and ionicity) was evaluated for a series of NLO materials, and, phenomenologically, the fine mixing of covalency and ionicity will benefit a balanced dij and LIDT. Chemical bonds with different interaction strengths were introduced simultaneously to mix the covalency and iconicity finely, and three new IR NLO sulfides, A2Ba3Li6Ga28S49 (A = K, 1; Rb, 2; Cs, 3), were obtained. They exhibit a strong NLO efficiency (1.9-2.1 × AgGaS2 at 1064 nm and 0.5-0.6 × AgGaS2 at 1910 nm) and high LIDTs (16.7-18.0 × AgGaS2), which fulfill the criteria of being promising IR NLO candidates. This study provides a new method for designing high-performance IR NLO materials based on the topological features of the ELF.

19.
Cancer Cell Int ; 21(1): 500, 2021 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-34535132

RESUMO

BACKGROUND: The prognosis of hepatocellular carcinoma (HCC) is not optimistic. Our study focused on present inflammatory markers, including the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), gamma-glutamyl transpeptidase-to-platelet ratio (GPR), aspartate aminotransferase-to-lymphocyte ratio (ALR) and fibrinogen-to-albumin ratio (FAR), and explored their optimal combination for the prognosis of HCC after resection. METHODS: A total of 347 HCC patients who underwent curative resection were enrolled. The optimal cutoff values of the inflammatory markers were calculated using receiver operating characteristic (ROC) curve analysis, and used to divide patients into two groups whose differences were compared by Kaplan-Meier analysis. Cox univariate and multivariate analyses were used to analyze the independent prognostic inflammatory markers. The χ2 test was chosen to determine the relationship between independent prognostic inflammatory markers and clinicopathological features. We created combined scoring models and evaluated them by Cox univariate and multivariate methods. The concordance index (C-index), Akaike information criterion (AIC) and likelihood ratio were calculated to compare the models. The selected optimal inflammatory markers and their combinations were tested in different stages of HCC by Kaplan-Meier analysis. RESULTS: The ALR and GPR were independent prognostic factors for disease-free survival (DFS); the ALR, PLR, and GPR were independent prognostic factors for overall survival (OS). The proposed GPR and ALR-GPR-PLR score models were independent predictors for DFS and OS, respectively. CONCLUSION: The preoperative GPR and ALR-GPR-PLR score models were independent predictors for DFS and OS, respectively, and performed well in stratifying patients with HCC. The higher the score in the model was, the worse the prognosis.

20.
Front Microbiol ; 12: 698930, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34484142

RESUMO

Healthy cotton samples were collected and 93 endophytic fungal strains were isolated: 23 strains from the roots and 70 strains from the stems. Morphological characterization and ITS sequence analysis were used for the identification of these isolates. The results showed that the 93 strains including 20 species were highly diverse in terms of their taxonomy. Simpson's and Shannon's diversity indices were 0.915 and 3.848, respectively. Fusarium and Alternaria were the two dominant genera, constituting 19.4% of the total strains. Then, 72 spore-producing strains were tested for the suppression of cotton Verticillium wilt (CVW) caused by Verticillium dahliae in a greenhouse. Five strains exhibited effective suppression of CVW with average efficacy values higher than 50%. One of the effective strains, namely, Fusarium proliferatum 10R-7, was selected for the investigation of the role of fusaric acid, a secondary metabolite of strain 10R-7, in the suppression of V. dahliae and CVW. The results showed that F. proliferatum 10R-7 could produce fusaric acid, and this metabolite exhibited 100% inhibition of mycelial growth of V. dahliae at concentrations higher than 20 µg/ml. However, fusaric acid at 2.5 to 80 µg/ml was not effective in the suppression of CVW, compared with the control treatment with V. dahliae alone. F. proliferatum 10R-7 was labeled with green fluorescent protein (GFP), and the GFP-tagged strain was found to be able to colonize inside the taproots of cotton, suggesting that F. proliferatum 10R-7 is a true endophyte of cotton and endophytic colonization may play a role in the suppression of infection of cotton by V. dahliae.

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