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1.
Support Care Cancer ; 28(1): 373-380, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31049672

RESUMO

BACKGROUND/OBJECTIVES: The assessment of nutritional status and the quality of life in patients with gastric cancer has become one of the important goals of current clinical treatment. The purpose of this study was to assess the nutritional status in hospitalized gastric cancer patients by using patient-generated subjective global assessment (PG-SGA) and to analyze the influence of nutritional status on the patients' quality of life (QOL). METHODS: We reviewed the pathological diagnosis of gastric cancer for 2322 hospitalized patients using PG-SGA to assess their nutritional status and collected data on clinical symptoms, the anthropometric parameters (height, weight, body mass index (BMI), mid-arm circumference (MAC), triceps skin-fold thickness (TSF), and hand-grip strength (HGS). We also collected laboratory data (prealbumin, albumin, hemoglobin) within 48 h after the patient was admitted to the hospital. The 30-item European Organization for Research and Treatment of Cancer Core Quality of Life Questionnaire (EORTC QLQ-C30) was used for QOL assessment in all patients. RESULTS: By using PG-SGA, we found 80.4% of the patients were malnourished (score ≥ 4) and 45.1% of the patients required urgent nutritional support (score ≥ 9). In univariate analysis, old age (> 65 years, p < 0.001), female (p = 0.007), residence in a village (p = 0.004), a lower level of education (p < 0.001), and self-paying (p < 0.001) were indicated as risk factors of patients with gastric cancer to be suffering from severe malnutrition. There was a negative correlation between PG-SGA and various nutritional parameters (p < 0.05). The quality of life was significantly different in gastric cancer patients with different nutritional status (p < 0.01). CONCLUSION: Malnutrition of hospitalized patients with gastric cancer in China is common and seriously affects the patients' quality of life. The nutritional status should be evaluated in a timely manner and reasonable nutritional intervention should be provided as soon as possible. The PG-SGA was fit for using as a clinical nutrition assessment method, being worthy of clinical application.

2.
Ecotoxicol Environ Saf ; 188: 109905, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31706245

RESUMO

Cooking oil fumes-derived PM2.5 (COFs-derived PM2.5) is the main source of indoor pollution. Exposure to COFs-derived PM2.5 can cause oxidative stress and affect angiogenesis. Here we investigated the roles of vitamin D3 (VD3) in protecting tubule formation injury induced by COFs-derived PM2.5, and the roles of ROS/NLRP3/VEGF signaling pathway in the effects. Human umbilical vein endothelial cells (HUVECs) were exposed to 0 (1‰ DMSO), 1000 nmol/l VD3, 100 µg/ml PM2.5, and 1000 nmol/l VD3 + 100 µg/ml PM2.5, respectively. Cell viability and tube formation, as well as protein and mRNA levels were measured. The results showed that exposure of COFs-derived PM2.5 dose-and time-dependently reduced the viability of HUVECs, increased the levels of mitochondrial and intracellular ROS, and changed the mitochondrial membrane potential level. While co-incubation with VD3 rescued these adverse effects. Both Western blot and real-time PCR (RT-PCR) showed that the expressions of NLRP3, caspase-1, Interleukin (IL)-1ß, and IL-18 in COFs-derived PM2.5 exposure group increased significantly, which could be effectively decreased by co-incubation with VD3. COFs-derived PM2.5 exposure could also reduce the expression of VEGF, while co-incubating HUVECs with VD3 evidently up-regulated the protein level of VEGF in HUVECs. In addition, COFs-derived PM2.5 could also inhibit the tube formation of HUVECs in vitro, which could be effectively rescued by the co-incubation of VD3. Our study proved that COFs-derived PM2.5 could damage the tubule formation of HUVECs in vitro, which could be effectively rescue by co-incubation with VD3, in which processes the ROS/NLRP3/VEGF signaling pathway played a crucial role. It provides a new theoretical basis for further study on the toxicity of PM2.5 to umbilical cord blood vessels.

3.
J Phys Condens Matter ; 32(6): 065306, 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-31671411

RESUMO

Recently, a novel two-dimensional (2D) semiconductor, InSe, has attracted great attention due to its potential applications in optoelectronic devices and field effect transistors. In this study, phonon-limited mobility is investigated by the first-principles calculation. At 300 K, the intrinsic electron mobilities calculated from the electron-phonon coupling (EPC) matrix element are as high as [Formula: see text] (zigzag direction) and [Formula: see text] [Formula: see text] (Armchair direction), respectively. The deformation potential theory (DPT) based on longitudinal acoustic and optical phonon scattering is also employed to investigate electron mobility. The mobility from optical phonon scattering is much higher than that from longitudinal acoustic phonon scattering. If the polarization characteristics of InSe are not considered, the electron mobility calculated from EPC matrix element is closed to that from the longitudinal acoustic phonon DPT. In this study, we have also investigated the effect of polarization properties in 2D InSe on electron mobility. At 300 K, the electron mobility for including Fröhlich interaction is reduced to [Formula: see text] and [Formula: see text] [Formula: see text]. Therefore, the electron mobility for InSe is controlled by the scattering from polar phonons. The mobility can be increased to [Formula: see text] and [Formula: see text] [Formula: see text] under 4% biaxial strain. This result is compared with the experiment, and some disagreements are explained.

4.
Food Chem ; : 125866, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31784068

RESUMO

The composition of donkey milk is similar to that of human milk. However, the lipid content in donkey milk is lower than that in human milk. Thus far, the lipid composition of donkey milk during lactation has not been well-studied. Through mass spectroscopy-based quantitative lipidomics, we analyzed lipids in donkey colostrum (DC) and mature milk (DM). Thirteen subclasses of 335 lipids were identified in both DC and DM; 60 lipids - 17 upregulated and 43 downregulated - were differentially regulated between DM and DC (Variable Importance in Projection >1, P < 0.05), demonstrating that lipid composition changed with lactation. These different lipids were involved in 19 metabolic pathways, of which glycerophospholipid, linoleic acid, alpha-linolenic acid, glycosylphosphatidylinositol-anchor, glycerolipid, and arachidonic acid metabolism were the most relevant. Our results provide insights into quantitative alterations in donkey milk lipids during lactation, development of donkey milk products, and screening of potential biomarkers.

5.
J Sci Food Agric ; 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31802504

RESUMO

BACKGROUND: Methoxyfenozide possesses efficacy against to a variety of lepidopteron pests, including the major pests in cauliflower and tea, it is of great importance to generalize the practical use of methoxyfenozide in the field. RESULTS: An efficient method was developed and validated in both vegetable matrix and extract-rich matrix (cauliflower and tea) using modified QuEChERS combined with UPLC-MS/MS analysis. The recoveries in cauliflower, made tea and tea shoots range from 94.5-108.0 %, 85.0-91.6 % and 77.3-82.0 % respectively, with the relative standard deviations (RSDs) below 17.3 % for all the cases. The field results showed that methoxyfenozide dissipated in cauliflower with the half-lives (t1/2 ) at 2.5-3.5 days, and in tea with t1/2 at 1.2 days. In combination of above experimental data and statistical food intake value, the risk quotient (RQ) values were significantly lower than 1. CONCLUSION: The quantification method of methoxyfenozide in cauliflower or tea has not been established until this study. The dissipation and dietary exposure risk assessment of methoxyfenozide in cauliflower and tea were investigated in field. Methoxyfenozide dissipated rapidly in cauliflower despite of the different climates, and it dissipated faster in tea. The dietary risk of methoxyfenozide through cauliflower or tea was negligible to humans. This study not only provides guidance for the safe use of methoxyfenozide, but also serves as a reference for the establishment of maximum residue limits (MRLs) in China. This article is protected by copyright. All rights reserved.

6.
Hypertension ; 74(6): 1532-1540, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31679423

RESUMO

The association between primary aldosteronism (PA) and obstructive sleep apnea (OSA) has been a matter of debate. 2016 Endocrine Society guideline recommends screening for PA all hypertensive patients with OSA. We designed a multicenter, multiethnic, cross-sectional study to evaluate the prevalence of PA in patients with OSA and the prevalence of OSA in unselected patients with PA. Two hundred and three patients with OSA (102 whites and 101 Chinese) were screened for PA, and 207 patients with PA (104 whites, 100 Chinese, and 3 of African descent) were screened for OSA by cardiorespiratory polygraphy. Eighteen patients with OSA (8.9%) had PA (11.8% of white and 5.9% of Chinese ethnicity). In patients without other indications for PA screening, the prevalence of PA dropped to 1.5%. The prevalence of OSA in patients with PA was 67.6%, consistent in both white and Chinese patients. A correlation between aldosterone levels and apnea/hypopnea index was observed in white patients with PA (R2=0.225, P=0.016) but not in Chinese patients. Multinomial logistic regression confirmed a significant and independent association between plasma aldosterone levels and moderate to severe OSA diagnosis in white patients (odds ratio, 1.002; P=0.002). In conclusion, aldosterone levels may contribute to the severity of OSA in white patients with hyperaldosteronism, but patients with OSA are not at high risk of PA. Results of the present study challenge the current recommendation of the Endocrine Society guideline that all patients with OSA should be screened for PA, irrespective of the grade of hypertension.

7.
Food Res Int ; 126: 108612, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31732041

RESUMO

To shorten the fermentation time and reduce the contamination with heterozygous bacteria during the process of fermenting rice noodles, pure bacterial fermentation combined with semidry flour milling was used to produce fermented rice noodles. The changes in the pH value and microbial community diversity of the rice slurries, the physicochemical properties of the rice flours, and the texture, cooking qualities, sensory qualities and volatile components of the rice noodles prepared with different fermentative methods were investigated. The results showed that the pure bacterial fermentation required 18 h to reach a stable pH value, while the natural fermentation required 54 h. In addition, compared with naturally fermented rice slurry, the bacterial community diversity of the pure bacterial fermented rice slurry was significantly lower, while the fungal community diversity did not differ significantly. Significant differences were observed in the content of the damaged starch, amylose and protein between semidry milled pure bacterial fermented (SP) and wet milled naturally fermented (WN) rice flours (P < .05), while these differences had no significant effect on the texture and cooking qualities of the SP and WN rice noodles (P > .05). In addition, the types of volatile components of the SP noodles were significantly lower than those of other noodles, while the primary volatile component categories of the SP noodles were consistent with that of the WN noodles, and they had a similar flavoring substance.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31732574

RESUMO

Xanthomonas campestris pv. campestris (Xcc) is the causative agent of black rot disease in crucifer plants. This Gram-negative bacterium utilises type III secretion system (T3SS), encoded by the hrp gene cluster, to aid with its resistance to host defences and ability to cause disease. The T3SS injects a set of proteins known as effectors into host cells that come into contact with the bacterium. T3SS is essential for the virulence and hypersensitive response (HR) of Xcc, making it a potential target for disease control strategies. Using a unique and straightforward high-throughput screening method, we examined a large collection of diverse small molecules for their potential to modulate T3SS without affecting the growth of Xcc. Screening of 13,129 different compounds identified ten small molecules that had a significant inhibitory influence on T3SS. Moreover, qRT-PCR assays demonstrated that all of the ten compounds repress expression of the hrp genes. Interestingly, the effect of these small molecules on hrp genes may be through the HpaS and ColS sensor kinase proteins that are key to the regulation of T3SS in planta Five of the compounds were also capable of inhibiting Xcc virulence in a Chinese radish leaf clipping assay. Furthermore, seven of the small molecules significantly weakened the HR in non-host pepper plant challenged with Xcc Taken together, these small molecules may provide potential tool compounds for further development of anti-virulence agents that could be used in the disease control of the plant pathogen Xcc IMPORTANCE The bacterium Xanthomonas campestris pv. campestris (Xcc) is known to cause black rot disease in many socio-economically important vegetable crops worldwide. The management and control of black rot disease has been tackled with chemical and host resistance methods to varying success. This has motivated the development of alternative methods for preventing this disease. Here, we identify a set of novel small-molecules capable of inhibiting Xcc virulence in plant test, which may represent leading compounds for further development of anti-virulence agents that could be used in the control of black rot disease.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31739449

RESUMO

Particulate matter with a diameter of less than 2.5 µ m (PM2.5) has damaged public health globally for a decade. Accurate forecasts of PM2.5 concentration can provide early warnings to prevent the public from hazard exposure. However, existing methods have not considered the available spatiotemporal data sufficiently due to their architecture or inadequate input, and most neglected wind impact on spatiotemporal correlation when selecting related sites. To fill this gap, we proposed a long short-term memory-convolutional neural network based on dynamic wind field distance (LSTM-CNN-DWFD) to predict PM2.5 concentration of a specific site for the next 24 h. A KNN method based on dynamic wind field distance was developed and applied to select highly related sites considering wind impact. A local stateful LSTM model was employed to capture temporal correlations in historical air quality and meteorological data for each related site. Then, these temporal features were integrated as a spatiotemporal matrix, and input into CNN for extracting spatiotemporal correlation features. Weather forecasts were also integrated into the model to promote accuracy. Hourly PM2.5 data from 36 monitoring sites in Beijing, China collected from 1 May 2014 to 30 April 2015 were used as experimental dataset. Six-fold rolling origin method was employed to conduct experiments on each site, and the results of 216 experiments validated the performance of the proposed LSTM-CNN-DWFD model. The mean R 2 values of the next 1-6 h prediction were 0.85, 0.81, 0.76, 0.70, 0.64, and 0.59, respectively, showing a decrease trend, indicating that the prediction accuracy decreases as the prediction time increases. Comparisons of LSTM-CNN-DWFD results to results from six other methods show that it delivered higher accuracy PM2.5 predictions, with the mean RMSE (MAE) of 1-6, 7-12, and 13-24 h prediction were 43.90 (29.17), 57.89 (42.16), and 63.14 (47.64), respectively. The results also demonstrate that the sites selected based on dynamic wind field distance are more related to the central site than that based on geographical distance, also contributing to prediction accuracy.

10.
Int J Pharm ; : 118864, 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31765782

RESUMO

Natural phenolic drugs have good safety and various biological activities. However, poor bioavailability and inadequate bioactivity severely limit their application. A novel composite alkali polysaccharide nanovesicle was formed with supramolecule- and nano- technologies to efficiently deliver natural phenolic antitumor drugs. Alkali polysaccharide nanovesicles (ASDLM) containing supramolecular diferuloylmethane (DLM) had the additional effects of alkali polysaccharide nanovesicles and supramolecules of drug and high-molecular-weight polymers. DLM was isolated from the external environment when double loaded by cyclodextrin and nanovesicles; The nanosize, negative/positive charges and supramolecular structure were beneficial attributes that helped to increase the bioavailability and antitumor activity; supramolecular DLM-loaded nanovesicles made of natural biodegradable excipients showed good safety. Compared to free DLM, ASDLM exhibited superior physicochemical characteristics, favorable changes in the in vitro/in vivo kinetic performance, a possible in vitro-in vivo correlation, enhanced in situ gastrointestinal absorption, increased bioavailability, and an elevated anti-lung cancer efficiency. Composite alkali polysaccharide nanovesicles conjugated with supramolecular-/nano- technology may provide a valuable platform for the oral delivery of botanical drugs to meet clinical requirements.

11.
J Chromatogr A ; : 460725, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31767260

RESUMO

In this work, a new analytical method based on polydopamine functionalized magnetic graphene (PDA@MG) adsorbent material has been developed to determine three triazole fungicides in water samples. As previous step, a novel polydopamine functionalized PDA@MG adsorbent material has been successfully prepared, which was characterized by fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscope (TEM), and vibrating sample magnetometer (VSM). Based on this novel material, a new magnetic solid phase extraction (MSPE) method coupled with high performance liquid chromatography (HPLC) has been established for the determination of triazole fungicides in water samples. The main factors which could affect the experimental results were optimized. Under the optimal conditions, good linarites has been achieved in the range of 0.2-50 µg L-1, with the correlation coefficients (R2) were between 0.9962 and 0.9996. The limits of detections (LODs) were 0.0048-0.0084 µg L-1, and the relative standard deviations (RSDs) were between 1.7% and 4.8%. In addition, enrichment factors (EFs) were 572-916 times, which showed triazole fungicides residues could be accurately extracted and analyzed in this way. In the final experiment, the established method was applied to the detection of target analyzes in water samples. Satisfied results could be obtained for tebuconazole, propiconazole, and flusilazole. The recoveries of five water samples were between 69.4% and 106.4%, and the RSD were between 1.0% and 6.5%. The development method is more easy, effective, green and environmental-friendly, and has potential for application.

12.
Colloids Surf B Biointerfaces ; : 110631, 2019 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-31776054

RESUMO

Cancer is a huge challenge humanity facing today, and single chemical treatments inevitably have shortcomings such as poor selectivity and large side effects. This paper constructed an egg yolk phospholipids modified molybdenum disulfide (MoS2) nanocarrier system for the treatment of tumors via the combination of chemotherapy and photothermal therapy. The lipid-modified layered MoS2 (MoS2-Lipid) nanocomposite was synthesized by simple physical adsorption. The lipid modification strongly enhanced the stability of MoS2 nanosheets and the nanocarrier has a large drug loading amount with pH dependent DOX release profile, an excellent photothermal property, and an ideal cellular uptake property. Therefore, we combined chemotherapy and photothermal therapy to treat tumors synergistically. Through in vitro cell experiments, pure nanocomposite had no obvious cytotoxicity to cells, and the synergistic treatment of tumors by chemotherapy and photothermal therapy was more effective than any single treatment. More importantly, in vivo experiments indicated that lipid modification enhanced the accumulation of the nanocarrier in mice tumors, thus a better photothermal performance could be seen compared with original MoS2 nanosheets. In summary, the MoS2-lipid nanocomposite is a promising nanocarrier for the treatment of tumors by chemo and photothermal therapy.

13.
Eur J Radiol ; 121: 108711, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31677544

RESUMO

PURPOSE: We developed and validated a radiomic model based on mammography and assessed its value for predicting the pathological diagnosis of Breast Imaging Reporting and Data System (BI-RADS) category 4 calcifications. MATERIALS AND METHODS: Patients with a total of 212 eligible calcifications were recruited (159 cases in the primary cohort and 53 cases in the validation cohort). In total, 8286 radiomic features were extracted from the craniocaudal (CC) and mediolateral oblique (MLO) images. Machine learning was used to select features and build a radiomic signature. The clinical risk factors were selected from the independent clinical factors through logistic regression analyses. The radiomic nomogram incorporated the radiomic signature and an independent clinical risk factor. The diagnostic performance of the radiomic model and the radiologists' empirical prediction model was evaluated by the area under the receiver operating characteristic curve (AUC). The differences between the various AUCs were compared with DeLong's test. RESULTS: Six radiomic features and the menopausal state were included in the radiomic nomogram, which discriminated benign calcifications from malignant calcifications with an AUC of 0.80 in the validation cohort. The difference between the classification results of the radiomic nomogram and that of radiologists was significant (p < 0.05). Particularly for patients with calcifications that are negative on ultrasounds but can be detected by mammography (MG+/US- calcifications), the identification ability of the radiomic nomogram was very strong. CONCLUSIONS: The mammography-based radiomic nomogram is a potential tool to distinguish benign calcifications from malignant calcifications.

14.
Lipids Health Dis ; 18(1): 199, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31729985

RESUMO

BACKGROUND: The triglyceride (TG) to high-density lipoprotein cholesterol (HDL-C) ratio (TG/HDL-C) has been suggested as a simple method to identify unfavorable cardiovascular (CV) outcomes in the general population. The aim of this study was to investigate the association between the TG/HDL-C ratio and all-cause and CV mortality in peritoneal dialysis (PD) patients. METHODS: We retrospectively analyzed patients on PD from November 1, 2005, to February 28, 2017, with a follow-up period lasting until May 31, 2017. The main outcomes were all-cause and CV mortality. RESULTS: Among the 973 PD patients, the mean age was 49.67 ± 14.58 (y). During a median follow-up period of 27.2 months (IQR = 13.4-41.5 months), 229 (23.5%) patients died, with 120 (12.3%) dying as a result of CV diseases. The median serum TG/HDL-C ratio was 1.11 (IQR = 0.71-1.80). In a multivariate Cox regression analysis, patients with higher TG/HDL-C ratio levels (tertile 3) had a higher incidence of CV mortality (adjusted HR = 2.12; 95% CI: 1.21-3.72; P = 0.009) and all-cause mortality (adjusted HR = 2.08; 95% CI: 1.37-3.14; P = 0.001) compared to patients in tertile 1. These associations persisted after excluding the patients who have already taken lipid-lowering medications. For older patients (> 60 years), each 1-unit higher baseline TG/HDL-C level was associated with a 48% (95% CI: 1.06-2.07; P = 0.021) increased risk of all-cause mortality and a 59% (95% CI: 1.03-2.45; P = 0.038) increased risk of CV mortality; however, this association was not observed in patients ≤60 years of age. CONCLUSIONS: A higher serum TG/HDL-C ratio was an independent predictor of all-cause and CV mortality in PD patients. Furthermore, an elevated TG/HDL-C ratio was significantly associated with higher all-cause and CV mortality in older PD patients.

15.
BMC Pediatr ; 19(1): 423, 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31707983

RESUMO

BACKGROUND: The initial episode of angioedema in children can be potential life-threatening due to the lack of prompt identification and treatment. We aimed to analyze the factors predicting the severity and outcomes of the first attack of acute angioedema in children. METHODS: This was a retrospective study with 406 children (< 18 years) who presented in the emergency department (ED) with an initial episode of acute angioedema and who had subsequent follow-up visits in the out-patient department from January 2008 to December 2014. The severity of the acute angioedema was categorized as severe (requiring hospital admission), moderate (requiring a stay in the short-term pediatric observation unit [POU]), or mild (discharged directly from the ED). The associations among the disease severity, patient demographics and clinical presentation were analyzed. RESULT: In total, 109 (26.8%) children had severe angioedema, and the majority of those children were male (65.1%). Most of the children were of preschool age (56.4%), and only 6.4% were adolescents. The co-occurrence of pyrexia or urticaria, etiologies of the angioedema related to medications or infections, the presence of respiratory symptoms, and a history of allergies (asthma, allergic rhinitis) were predictors of severe angioedema (all p < 0.05). Finally, the duration of angioedema was significantly shorter in children who had received short-term POU treatment (2.1 ± 1.1 days) than in those who discharged from ED directly (2.3 ± 1.4 days) and admitted to the hospital (3.5 ± 2.0 days) (p < 0.001). CONCLUSION: The co-occurrence of pyrexia or urticaria, etiologies related to medications or infections, the presence of respiratory symptoms, and a history of allergies were predictors of severe angioedema. More importantly, short-term POU observation and prompt treatment might be benefit for patients who did not require hospital admission.

16.
Nanotechnology ; 31(8): 085702, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31675728

RESUMO

The cross-sectional shape of the nanotube is a key factor governing fundamental mechanical properties of the nanotube and the nanotube forest. In contrast to most circular nanotubes, in the present work, we demonstrate that the holey phenine nanotubes have polygonal cross sections with diameter-dependent number of sides. The non-circular cross section is attributed to the high twistability of the continuous C-C chains in the phenine nanotube. Consequently, the phenine nanotube forest has a square lattice structure rather than the regular hexagonal lattice of the carbon nanotube forest, resulting in a smooth buckling process under biaxial compression. The buckling pattern of the phenine nanotube forest is highly ordered with the orientation determined by the initial dislocation that frequently appears in the phenine nanotube forest.

17.
J Dairy Sci ; 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31677837

RESUMO

Donkey milk has been widely shown to be an ideal substitute for human milk because of its similar composition. However, alterations to the composition of donkey milk during lactation have not been well studied. In this study, untargeted metabolomics with ultra-high-performance liquid tandem chromatography quadrupole time-of-flight mass spectrometry were used to analyze and compare the metabolites in donkey colostrum (DC) and mature milk (DMM). Two hundred seventy metabolites were characterized in both DC and DMM. Fifty-two of the metabolites in the DC were significantly different from those in the DMM; 8 were downregulated and 44 were upregulated. This demonstrated that the composition of the donkey milk changed with lactation. Additionally, the interactions and metabolic pathways were further analyzed to explore the mechanisms that altered the milk during lactation. Our results provide comprehensive insights into the alterations in donkey milk during lactation. The results will aid in future investigations into the nutrition of donkey milk and provide practical information for the dairy industry.

18.
Int J Mol Med ; 44(6): 2103-2112, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31638180

RESUMO

Recent studies have suggested that calcium­activated potassium channel (KCa) agonists increase the proportion of mouse embryonic stem cell­derived cardiomyocytes and promote the differentiation of pacemaker cells. In the present study, it was hypothesized that adipose­derived stem cells (ADSCs) can differentiate into pacemaker­like cells via overexpression of the SK4 gene. ADSCs were transduced with a recombinant adenovirus vector carrying the mouse SK4 gene, whereas the control group was transduced with GFP vector. ADSCs transduced with SK4 vector were implanted into the rat left ventricular free wall. Complete atrioventricular block (AVB) was established in isolated perfused rat hearts after 2 weeks. SK4 was successfully and stably expressed in ADSCs following transduction. The mRNA levels of the pluripotent markers Oct­4 and Sox­2 declined and that of the transcription factor Shox2 was upregulated following SK4 transduction. The expression of α­actinin and hyperpolarization­activated cyclic nucleotide­gated potassium channel 4 (HCN4) increased in the SK4 group. The hyperpolarizing activated pacemaker current If (8/20 cells) was detected in ADSCs transduced with SK4, but not in the GFP group. Furthermore, SK4 transduction induced the expression of p­ERK1/2 and p­p38 MAPK. In the ex vivo experiments, the heart rate of the SK4 group following AVB establishment was significantly higher compared with that in the GFP group. Immunofluorescence revealed that the transduced ADSCs were successfully implanted and expressed HCN4 in the SK4 group. In conclusion, SK4 induced ADSCs to differentiate into cardiomyocyte­like and pacemaker­like cells via activation of the extracellular signal­regulated kinase 1/2 and p38 mitogen­activated protein kinase pathways. Therefore, ADSCs transduced with SK4 may be used to generate biological pacemakers in ex vivo rat hearts.

19.
J Agric Food Chem ; 67(44): 12322-12332, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31638792

RESUMO

The objective of the present study was to reveal the antibacterial mechanism of lactobionic acid (LBA) against methicillin-resistant Staphylococcus aureus (MRSA) using quantitative proteomics by sequential window acquisition of all theoretical mass spectra (SWATH-MS) to analyze 100 differentially expressed proteins after LBA treatment. Furthermore, multiple experiments were conducted to validate the results of the proteomic analysis including reactive oxygen species (ROS), virulence-associated gene expression, and the relative quantification of target proteins and genes by parallel reaction monitoring and quantitative real-time PCR. Combining the ultrastructure observations, proteomic analysis, and our previous research, the mode of LBA action against MRSA was speculated as cell wall damage and loss of membrane integrity; inhibition of DNA repair and protein synthesis; inhibition of virulence factors and biofilm production; induction of oxidative stress; and inhibition of metabolic pathways. These results suggest potential applications for LBA in food safety and pharmaceuticals, considering its multitarget effects against MRSA.


Assuntos
Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Dissacarídeos/farmacologia , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Proteínas de Bactérias/química , Proteínas de Bactérias/metabolismo , Dissacarídeos/genética , Dissacarídeos/metabolismo , Espectrometria de Massas , Staphylococcus aureus Resistente à Meticilina/química , Staphylococcus aureus Resistente à Meticilina/genética , Staphylococcus aureus Resistente à Meticilina/metabolismo , Testes de Sensibilidade Microbiana , Proteômica
20.
Artigo em Inglês | MEDLINE | ID: mdl-31625219

RESUMO

BACKGROUND: Inappropriate sinus tachycardia (IST) remains a clinical challenge because patients often are highly symptomatic and not responsive to medical therapy. OBJECTIVE: To study the safety and efficacy of stellate ganglion (SG) block and cardiac sympathetic denervation (CSD) in patients with IST. METHODS: Twelve consecutive patients who had drug-refractory IST (10 women) were studied. According to a prospectively initiated protocol, five patients underwent an electrophysiologic study before and after SG block (electrophysiology study group). The subsequent seven patients had ambulatory Holter monitoring before and after SG block (ambulatory group). All patients underwent SG block on the right side first, and then on the left side. Selected patients who had heart rate reduction ≥15 beats per minute (bpm) were recommended to consider CSD. RESULTS: The mean (SD) baseline heart rate (HR) was 106 (21) bpm. The HR significantly decreased to 93 (20) bpm (P = .02) at 10 minutes after right SG block and remained significantly slower at 97(19) bpm at 60 minutes. Left SG block reduced HR from 99 (21) to 87(16) bpm (P = .02) at 60 minutes. SG block had no significant effect on blood pressure or HR response to isoproterenol or exercise (all P > .05). Five patients underwent right (n = 4) or bilateral (n = 1) CSD. The clinical outcomes were heterogeneous: one patient had complete and two had partial symptomatic relief, and two did not have improvement. CONCLUSION: SG blockade modestly reduces resting HR but has no significant effect on HR during exercise. Permanent CSD may have a modest role in alleviating symptoms in selected patients with IST.

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