Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.453
Filtrar
1.
PLoS One ; 15(1): e0227182, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31914138

RESUMO

OBJECTIVES: Fluctuation is a common but neglected phenomenon of physical activity (PA) behavior. This study aimed to explore the psychosocial profiles of PA fluctuation in office employees, and to examine the association of latent profiles with demographics and PA level. METHOD: 434 Chinese office employees who were identified as PA fluctuators (M = 32.4 years, SD = 6.9, 55.5% female) completed a cross-sectional online survey covering demographics, PA behavior, and six psychosocial indicators (self-efficacy, planning, action control, affective attitude, social support, and perceived barriers). Latent profile analysis was used to determine PA fluctuators' psychosocial profiles. Associated factors of profile membership were identified with multinomial logistic regression. RESULTS: The two-profile model (uncommitted vs. moderately committed) was selected as the best solution. The moderately committed group (n = 346, 79.7%) possessed a more active mindset by reporting significantly higher scores of self-efficacy (t = 9.42 p < .001), planning (t = 16.33 p < .001), action control (t = 14.55 p < .001), affective attitude (t = 13.33 p < .001), and social support (t = 11.50 p < .001) compared with the uncommitted group (n = 88, 20.3%). Results from a multinomial logistic regression showed that the moderately committed profile was associated with normal weight status (OR = 2.00, p< .05), having a medium managerial position (OR = 2.54, p< .01), and high level of moderate to vigorous PA behavior (OR = 4.85, p< .001). CONCLUSIONS: These findings demonstrate the variability of PA fluctuators' mindsets. Future tailored interventions are recommended to promote PA behavior for this population based on the categorization from the present study.

2.
Sci Total Environ ; 713: 136530, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31951845

RESUMO

This study investigates anaerobically-digested sludge (ADS) disintegration by activated peroxymonosulfate (PMS) with transition metal ions of Co2+, Cu2+, Fe2+, and Mn2+ (PMS-Me2+). The activating performances of Me2+ are quantitatively compared by capillary suction time (CST), extracellular polymeric substances (EPS), bound water content (BWC), particle size distribution, and metal speciation. At Me2+ dose of 1.2 mmol/g VSS, PMS-Fe2+ and PMS-Cu2+ achieve the lowest normalized CST, i.e., CST/CST0, of 0.47, and the higher normalized CST values of 0.71 and 0.74 are observed for PMS-Mn2+ and PMS-Co2+, respectively. BWC shows little extent of decrease upon PMS-Me2+ oxidation, and the most significant decrease from 89.5% to 88.3% is observed for PMS-Mn2+. PMS-Co2+ contributes to the decrease of DOC in total EPS fractions (DOCtot) from 698.0 mg/L to 496.6 mg/L, whereas the increased DOCtot to 713.6, 734.4, and 755.0 mg/L is observed with the introduction of Cu2+, Fe2+, and Mn2+, respectively. Fe2+ tends to transform to Fe3+ and the coagulation effect increases the median particle diameter (D50) from 15.8 µm to 91.1 µm. Comparatively, much lower D50 values of below 20.0 µm are observed for other divalent ions. The European Community Bureau of Reference (BCR) sequential extraction method is used to analyze the metal speciation in ADS sediment after PMS-Me2+ disintegration. The dominant species of Co and Mn are acid extractable fractions with the ratios of 91.0% and 87.3%, whereas the main Fe and Cu species are observed to be residual and oxidizable fractions. The pre-captured Me2+ ions with 50-days aging interestingly exhibit activating efficacy towards PMS, and CST values were observed to decrease by 11.5%, 30.5%, 11.8%, and 27.3% with the presence of pre-captured Co2+, Cu2+, Fe2+, and Mn2+ at 1.2 mmol/g VSS. This study proposes the potentially valuable strategy for the disintegration and dewatering of sludge with high contents of transition metal ions.

3.
Sci Total Environ ; 713: 136570, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31954245

RESUMO

Wastewater reclamation and reuse is considered an attractive and practical method for coping with water scarcity. However, the presence of micropollutants in reclaimed water, including endocrine disrupting chemicals (EDCs), is a major public health concern. This study attempted to identify unknown EDCs with liver X receptor (LXRα) agonist/antagonist activities in reclaimed wastewater, using nuclear receptors binding extraction coupled with high-resolution mass spectrometry (NRBE-HRMS). In total, 105 compounds in the reclaimed wastewater exhibited LXRα-binding activity. Among them, two previously unknown LXRα-antagonist compounds, catechol and 4-acetamidoantipyrine, were identified, based on authentic standards. The two LXRα-antagonist compounds exhibited weak LXRα-antagonist activities in a yeast two-hybrid assay. Catechol and 4-acetamidoantipyrine inhibited the ß-galactosidase activity induced by 60 nM of TO901317 in an LXRα yeast assay, with IC20 values of 79,938.9 nM and 6286.4 nM, respectively. To the best of our knowledge, this is the first study to identify EDCs in reclaimed wastewater with LXRα-agonist/antagonist activity using the NRBE-HRMS method.

4.
Genome ; 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31928416

RESUMO

Chinese indigenous pig and Western commercial pig breeds show different patterns of lipid metabolism, fat deposition, and fatty acid composition, and have become vitally important models of energy metabolism and obesity in humans. To compare the mechanisms underlying lipid metabolism between Yorkshire (lean type) and Anqing six-end-white pigs (obese type), the liver transcriptomes of six castrated boars with a body weight of approximately 100 kg (three Yorkshire and three Anqing) were analyzed by RNA-seq. The total number of reads produced for each liver sample ranged from 47.05 to 62.6 million. Among 362 differentially expressed genes, 142 were up-regulated and 220 were down-regulated in Anqing six-end-white pigs. Based on these data, nine significantly enriched terms were identified by GO enrichment analysis, including lipid metabolic process and carboxylic acid metabolic process. Pathway analysis revealed three significantly enriched KEGG pathways including PPAR signaling pathway, steroid hormone biosynthesis, and retinol metabolism. Based on protein-protein interaction networks, multiple genes responsible for lipid metabolism were identified, such as PCK1, PPARA, and CYP7A1, and these were considered promising candidate genes that could affect porcine liver lipid metabolism and fat deposition. Our results provide abundant transcriptomic information that will be useful for animal breeding and biomedical research.

5.
Telemed J E Health ; 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31928505

RESUMO

Abstract Background/Introduction: The quality of maternal health care service is a crucial determinant of maternal morbidities and mortalities. This study aimed to explore feasibility and relative efficacy of WeChat (WC), of specialist team (ST) service, and of combined of both interventions (WC-ST) for improving the quality of maternal health care in China. Materials and Methods: A four-arm randomized controlled trial of 1,400 pregnant women was conducted in three hospitals in Chengdu, Southwest China, from December 2016 to October 2017. Eligible women were randomly assigned to either of three intervention groups or the control group (service as usual; SAU). Main outcome measures were satisfaction rate and uptakes of maternal health care service at 49 days postpartum based on questionnaire survey. Results: No significant differences in satisfaction rate were found among four groups at baseline (p = 0.981), and significant group differences were noted at 49 days postpartum (p < 0.001), with the highest rate from WC-ST group (98.6%), followed by that of ST (95.2%) and WC (91.6%) groups, and SAU group being the lowest (85.2%). The same pattern of group difference was observed in measures of health care uptake behaviors. Most health care uptake measures from the baseline to post-trial were significantly improved within each of the intervention groups, while most such measures in the control group were not different from baseline to post-trial. Discussion and Conclusions: The WC and ST service is feasible and potentially effective in improving the quality of maternal health care service in China. The study has revealed limitations and options for improvement in future main trial.

6.
Nutrients ; 12(1)2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31936273

RESUMO

Wikstroemia indica (L.) C.A. Mey. is used in traditional Chinese medicine to treat inflammatory diseases such as arthritis and bronchitis. In this study, we aimed to investigate the effects of an ethanolic extract of W. indica on cutaneous inflammation in mice with 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD). Dermal administration of W. indica ethanolic extract to DNCB-sensitized hairless mice with dermatitis, for two weeks, reduced erythema, scaling, and edema. Skin hydration was improved and transepidermal water loss was reduced at a W. indica concentration of 1%. Furthermore, W. indica also significantly reduced serum IgE and IL-4 concentrations in our mouse model. These results suggest that W. indica has potential as a topical treatment for AD and as an adjunctive agent to control AD.

7.
Kaohsiung J Med Sci ; 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31913555

RESUMO

China lacks data demonstrating associations of cervical neoplastic lesions with CD4 T-lymphocyte (CD4 cell) counts and antiretroviral therapy (ART) among HIV-infected women, suggesting relevant investigations are needed. A total of 545 HIV-infected women were enrolled in Yunnan, China, between 2011 and 2013. CD4 cell counts and ART were measured via medical records and cervical neoplastic lesions were measured by professional pathologists. Multivariable logistic models, which treated cervical intraepithelial neoplasia (CIN) 1+ and CIN2+ as outcomes, calculated adjusted odds ratio (aOR) of CD4 cell counts and ART. Subgroup analysis treating CIN1+ as the outcome was conducted by HIV infection duration (<4 vs ≥4 years), ethnicity (Han vs non-Han), and study site (Mangshi vs Kunming). The prevalence of CIN1+ and CIN2+ was 17.4% and 7.3%, respectively. Overall, 243 (44.6%) women had CD4 cell counts ≥500 cell/µL, 187 (34.3%) used ART for less than 2 years, and 236 (43.3%) used ART for at least 2 years. We found inverse associations of CIN1+ with CD4 cell counts (≥500 compared to <500 cells/µL: aOR = 0.46, 95% CI = 0.27-0.79) and ART use (<2 years: aOR = 0.43, 95% CI = 0.21-0.87; ≥2 years: aOR = 0.54, 95% CI = 0.27-1.10). Point estimates did not change substantially for CIN2+ but aORs of ART became nonsignificant. No significant interaction was observed for HIV infection duration. We found significant interaction between CD4 cell counts and ethnicity and study site in relation to CIN1+. Our study suggests potential protective effects of high CD4 cell counts against cervical neoplastic lesions among HIV-infected women, whereas associations of ART are less consistent.

8.
J Agric Food Chem ; 68(2): 530-540, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31891490

RESUMO

The influence of ß-hydroxy-ß-methylbutyrate (HMB) on proliferation and differentiation of myogenic cells has been well-studied. However, the role of HMB in myofiber specification and potential mechanisms is largely unknown. Thus, the objective of this research was to explore the role of HMB supplementation in myofiber specification. Results showed that HMB treatment significantly increased the fast MyHC protein level (mice: 1.59 ± 0.08, P < 0.01; C2C12: 2.26 ± 0.11, P < 0.001), decreased the slow MyHC protein level (mice: 0.76 ± 0.05, P < 0.05; C2C12: 0.52 ± 0.02, P < 0.001), and increased the miR-199a-3p level (mice: 4.93 ± 0.37, P < 0.001; C2C12: 11.25 ± 0.57, P < 0.001). Besides, we also observed that HMB promoted the activity of glycolysis-related enzymes and reduced the activities of oxidation-related enzymes in mice and C2C12 cells. Overexpression of miR-199a-3p downregulated the slow MyHC protein level (0.71 ± 0.02, P < 0.01) and upregulated the fast MyHC protein level (2.13 ± 0.09, P < 0.001), while repression of miR-199a-3p exhibited the opposite effect. Target identification results verified that miR-199a-3p targets the 3'UTR of the TEA domain family member 1 (TEAD1) to cause its post-transcriptional inhibition (0.41 ± 0.07, P < 0.01). Knockdown of TEAD1 exhibited a similar effect with miR-199a-3p on myofiber specification. Moreover, suppression of miR-199a-3p blocked slow-to-fast myofiber type transition induced by HMB. Together, our finding revealed that miR-199-3p is induced by HMB and contributes to the action of HMB on slow-to-fast myofiber type conversion via targeting TEAD1.


Assuntos
MicroRNAs/metabolismo , Fibras Musculares Esqueléticas/efeitos dos fármacos , Mioblastos Esqueléticos/efeitos dos fármacos , Valeratos/farmacologia , Regiões 3' não Traduzidas , Animais , Diferenciação Celular/efeitos dos fármacos , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Camundongos , MicroRNAs/genética , Fibras Musculares Esqueléticas/citologia , Fibras Musculares Esqueléticas/metabolismo , Mioblastos Esqueléticos/citologia , Mioblastos Esqueléticos/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Regulação para Cima/efeitos dos fármacos
9.
Water Res ; 172: 115469, 2020 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-31954932

RESUMO

Drinking water purification and distribution processes shape the bacterial community in tap water; however, this study calls attention to the impact of source water on tap water bacterial community. Herein, paired source and tap water samples were collected from 46 drinking water supply systems in different watersheds across China, and high-throughput sequencing delineated that bacterial richness and diversity decreased and biogeographical distribution pattern weakened in tap water compared to source water. Despite the great changes of bacteria from source to tap, Bayesian-based SourceTracker analysis still verified that the proportional contributions of source water to shaping the tap water bacterial community ranged from 0% to 92.8% (49.73% ± 30.22% on average). This indelible contribution was further confirmed by the Mantel test (P < 0.001), Procrustes test (P < 0.001) and variance partition analysis, which showed that the source water together with geographical location explained 40.11% of tap water bacterial community variation. To explore the potential reasons that explain the great differences in the impact of source water among different samples, the universal shift pattern of bacterial communities from source to tap was summarized as a classification of dominant bacterial taxa: "sensitive taxa" versus "resistant taxa". The taxa including Planctomycetes, Verrucomicrobia, Acidobacteria, Chloroflexi, and δ-Proteobacteria could act as biomarkers to distinguish samples between source and tap water, and were classified as sensitive taxa. In contrast, α-Proteobacteria, ß-Proteobacteria, γ-Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes, and Cyanobacteria were classified as resistant taxa. The abundance of sensitive taxa was negatively correlated with the SourceTracker proportion, while the abundance of resistant taxa was positively correlated with the SourceTracker proportion (P < 0.01). Thus, variation in source water bacterial community could be responsible for the degree of impact on tap water. Our findings give notice that the impact of source water microbiomes must be taken more seriously, and sufficient source water protection and engineering control strategies should be implemented to prevent the biological consequences of source water on tap water.

10.
Retina ; 40(2): 322-332, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31972803

RESUMO

PURPOSE: This study aims to characterize quantitative optical coherence tomography angiography (OCTA) features of nonproliferative diabetic retinopathy (NPDR) and to validate them for computer-aided NPDR staging. METHODS: One hundred and twenty OCTA images from 60 NPDR (mild, moderate, and severe stages) patients and 40 images from 20 control subjects were used for this study conducted in a tertiary, subspecialty, academic practice. Both eyes were photographed and all the OCTAs were 6 mm × 6 mm macular scans. Six quantitative features, that is, blood vessel tortuosity, blood vascular caliber, vessel perimeter index, blood vessel density, foveal avascular zone area, and foveal avascular zone contour irregularity (FAZ-CI) were derived from each OCTA image. A support vector machine classification model was trained and tested for computer-aided classification of NPDR stages. Sensitivity, specificity, and accuracy were used as performance metrics of computer-aided classification, and receiver operation characteristics curve was plotted to measure the sensitivity-specificity tradeoff of the classification algorithm. RESULTS: Among 6 individual OCTA features, blood vessel density shows the best classification accuracies, 93.89% and 90.89% for control versus disease and control versus mild NPDR, respectively. Combined feature classification achieved improved accuracies, 94.41% and 92.96%, respectively. Moreover, the temporal-perifoveal region was the most sensitive region for early detection of DR. For multiclass classification, support vector machine algorithm achieved 84% accuracy. CONCLUSION: Blood vessel density was observed as the most sensitive feature, and temporal-perifoveal region was the most sensitive region for early detection of DR. Quantitative OCTA analysis enabled computer-aided identification and staging of NPDR.

11.
Artigo em Inglês | MEDLINE | ID: mdl-31974680

RESUMO

PURPOSE: Primary tumor (PT) and metastatic lymph node (MLN) status have a great influence on diagnosis and treatment of lung cancer. Our main purpose was to investigate the imaging characteristics of PT or MLN by applying the 18F-FDG PET dynamic modeling approach for non-small cell lung cancer (NSCLC). METHODS: Dynamic 18F-FDG PET scans were performed for 76 lung cancer patients, and 62 NSCLC cases were finally included in this study: 37 with newly diagnosed early and locally advanced lung cancer without distant metastases (group M0) and 25 metastatic lung cancer (group M1). Patlak graphic analysis (Ki calculation) based on the dynamic modeling and SUV analysis from conventional static data were performed. RESULTS: For PT, both KiPT (0.050 ± 0.005 vs 0.026 ± 0.004 min-1, p < 0.001) and SUVPT (8.41 ± 0.64 vs 5.23 ± 0.73, p < 0.01) showed significant higher values in group M1 than M0. For MLN, KiMLN showed significant higher values in M1 than M0 (0.033 ± 0.005 vs 0.016 ± 0.003 min-1, p < 0.01), while no significant differences were found for SUVMLN between M0 and M1 (4.22 ± 0.49 vs 5.57 ± 0.59, p > 0.05). Both SUV PT and KiPT showed significant high values in squamous cell carcinoma than adenocarcinoma, but neither SUVPT nor KiPT showed significant differences between EGFR mutants versus wild types. The overall Spearman analysis for SUV and Ki from different groups showed variable correlation (r = 0.46-0.94). CONCLUSION: The dynamic modeling for MLN (KiMLN) showed more sensitive than the static analysis (SUV) to detect metastatic lymph nodes in NSCLC, although both methods were sensitive for PT. This methodology of non-invasive imaging may become an important tool to evaluate MLN and PT status for patients who cannot undergo histological examination. CLINICAL TRIAL REGISTRATION: The clinical trial registration number is NCT03679936 (http://www.clinicaltrials.gov/).

12.
Wiley Interdiscip Rev RNA ; : e1584, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31925936

RESUMO

Osteoarthritis (OA) is a bone and joint disease characterized by progressive cartilage degradation. In the face of global trends of population aging, OA is expected to become the fourth most common disabling disease by 2020. Nevertheless, the detailed pathogenesis of OA has not yet been elucidated. Noncoding RNAs (ncRNAs), including long noncoding RNAs, microRNAs, and circular RNAs, do not encode proteins but have recently emerged as important regulators of apoptosis and autophagy of chondrocytes, thereby highlighting a potential role in chondrocyte injury leading to OA onset and progression. We here review recent findings on these regulatory roles of ncRNAs to provide new directions for research on the pathogenesis of OA and offer new therapeutic targets for prevention and treatment. This article is categorized under: RNA in Disease and Development > RNA in Disease RNA in Disease and Development > RNA in Development.

13.
Chemosphere ; 238: 124632, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31472355

RESUMO

Fenton (Fe2+ + H2O2) reagents acting to remove organic pollutants possess dual functions, including the oxidation by hydroxyl radicals and the coagulation of Fe(III). Previous papers have extensively studied the oxidation reactions by hydroxyl radicals, however, the coagulation role of Fenton for benzoic acid (BA) removal is not clear. Comparing three coagulation systems, it was found that Fenton coagulation possesses a significant advantage for the removal of BA. Through Fenton conditional experiments, results showed that with the increase of H2O2 dosage, not only was the Fenton oxidation effect improved, but the Fenton coagulation effect was also significantly enhanced. Interestingly, the flocs produced by in situ Fenton possess a better coagulation effect than an aged Fenton system when processing BA. To further explain these results, Zeta potential, Transmission electron microscope (TEM), X-ray diffraction (XRD), X-ray absorption fine structure (EXAFS) and Brunner-Emmet-Teller (BET) measurements were used for characterization, and we found that the flocs produced by Fenton possessed a smaller particle size, lower polymerization states and a larger specific surface area and pore volume, which exposed more active sites to create a better coagulation effect. Additionally, through Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and Gas chromatography-mass spectrometer (GC-MS), we found that in situ Fenton oxidation and coagulation have synergistic effects, and the carboxyl-containing intermediates produced by the Fenton oxidation of BA can be combined with hydroxyl active sites of the flocs produced by in situ Fenton, resulting in a better removal effect. Finally, Fenton oxidation increases oxygen/carbon (O/C) to promote Fenton coagulation, and in situ Fenton more fully utilizes the active sites on the flocs' surface.


Assuntos
Ácido Benzoico/análise , Peróxido de Hidrogênio/química , Ferro/química , Esgotos/química , Poluentes Químicos da Água/análise , Purificação da Água/métodos , Floculação , Radical Hidroxila/química , Oxirredução , Espectroscopia Fotoeletrônica , Difração de Raios X
14.
J Colloid Interface Sci ; 562: 279-286, 2020 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-31841887

RESUMO

The rational design of nanostructure is very important for improving the number and effective utilization of active sites of the electrocatalysts. Here, a core-shell nanostructure composed of ZIF-67 core and Co(OH)2 shell (ZIF-67@Co(OH)2) has been obtained by subjecting ZIF-67 nanocube to the optimal high temperature etching process. After refluxing and etching in ethanol/water mixed solution, the loose Co(OH)2 shell can be constructed based on the surface of etched ZIF nanocube, which provides the obviously abundant active cobalt sites and better contact for oxygen evolution reaction (OER). Compared to the whole hollow Co(OH)2 nanocube, the solid ZIF-67 core in ZIF-67@Co(OH)2 can be favorable for charge transfer and provide the stable structure. The synergistic effect between Co(OH)2 shell and ZIF-67 core under suitable etching regulation can realize the optimized electrocatalysis for OER. The performance measurements show that Co(OH)2-1 after refluxing 1 h demonstrates the excellent activity requiring 354 mV overpotential at the current density of 10 mA cm-2 and the good stability. The enhanced mechanism may be due to the formation of loose Co(OH)2 as shell with fully exposed active sites, as well as the synergistic effect between ZIF-67 core and Co(OH)2 shell. Therefore, the surface construction of active composites based on ZIF precursor may be a new strategy for efficient electrocatalysis for water splitting.

15.
J Cell Biochem ; 121(1): 596-608, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31407404

RESUMO

Cervical cancer is the third leading cause of female death in the world. Serum microRNAs (miRNAs) are currently considered to be valuable as noninvasive cancer biomarkers, but their role in the prognosis of cervical cancer has not been elucidated. We aimed to find serum miRNAs that can be used as prognostic factors for cervical cancer. A traumatic pathological biopsy is the only reliable method for determining the severity of cervical cancer currently. Thus, noninvasive diagnostic markers are needed. The serological expression of candidate miRNAs were measured in 90 participants, including 60 patients with cervical cancer and 50 patients with cervical intraepithelial neoplasia. Two patients with cervical cancer were excluded from the study because of lack of data. miRNAs were evaluated by quantitative reverse transcription polymerase chain reaction. miR-143/-4636 appeared specific for cervical cancer compared with cervical intraepithelial neoplasia (P < .001). The classification performance of validated miRNAs for cervical cancer [Area under the receiver operating characteristic curve (AUC) = 0.942] was better than that reached by squamous cell carcinoma antigen (SCC-Ag; AUC = 0.727). Poor-differentiation group has lower miR-143/-4636 levels in serum (P < .05). miR-4636 level was correlated gross tumor volume and the depth of invasion (P < .0001). In our study, we found a combination of miR-143 and miR-4636 that is independently and strongly associated with cervical cancer prognosis and can be used as a clinically prognostic factor.

16.
Ultrason Sonochem ; : 104861, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31796325

RESUMO

Emodin is a bioactive compound with strong anti-inflammatory and antioxidant properties. Micellar casein is casein concentrates close to the native state of casein micelles. The interaction of emodin and micellar casein under heat treatment in the absence and presence of ultrasound was investigated, and the properties of microencapsulated emodin in micellar casein were compared. Fluorescence experiments proved that the major interaction between emodin and micellar casein was through hydrophobic forces under heat treatment in the absence and presence of ultrasound. However, ΔH, ΔS and ΔG of emodin-casein complexation without sonication were higher than those with sonication, in contradiction to binding constants. The particle sizes of emodin-casein complexes in the presence of ultrasound were smaller than those without sonication, while the specific surface area showed an opposite trend. As to encapsulation, emodin-casein capsules under heat-sonication treatment showed higher antioxidant properties than those of heat treatment alone under similar experimental conditions. Interestingly, micellar casein-emodin encapsulation in the presence of ultrasound showed a lower release rate of emodin in gastrointestinal conditions than that without ultrasound at the emdoin concentration of 10 µmol per gram casein. Ultrasound has been shown to be a potential processing technology for customizing the release kinetics of bioactive compounds.

17.
Artigo em Inglês | MEDLINE | ID: mdl-31822393

RESUMO

BACKGROUND: Hepatic epithelioid hemangioendothelioma (HEH) is a rare tumor of vascular origin with an unknown etiology, a low incidence, and a variable natural course. We evaluated the management and prognosis of HEH from the Surveillance, Epidemiology and End Results (SEER) program and changes in treatment modalities of HEH over 30 years. METHODS: From 1973 to 2014 in the SEER database, we selected patients diagnosed with HEH. We analyzed the clinical characteristics, patterns of management, and clinical outcomes of patients with HEH. RESULTS: We identified 79 patients with HEH (median age: 54.0 years; male to female ratio: 1:2.6). The initial extent of disease was local in 22 (27.8%) patients, regional metastasis in 22 (27.8%), distant metastasis in 31 (39.2%) and unknown in 4 (5.1%). The median size of primary tumor was 3.85 cm (interquartile range, 2.50-7.93 cm). Among 74 patients with available management data, the most common management was no treatment (29/74, 39.2%), followed by chemotherapy only (22/74, 29.7%), liver resection-based (13/74, 17.6%), and transplantation-based therapy (6/74, 8.1%). The 5-year cancer-specific survival rate was 57.8%. Patients who underwent surgical treatment had significantly higher survival than those who underwent non-surgical treatment (5-year survival; 88% vs. 49%, P = 0.019). Multivariate analysis revealed that surgical therapy was the only independent prognostic factor for survival (hazard ratio: 0.20, P = 0.040). CONCLUSIONS: Resection or liver transplantation is worth considering for treatment of patients with HEH.

18.
Front Med ; 2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31784918

RESUMO

This study aimed to investigate the prevalence, clinical characteristics, and prognostic impact of 1p32.3 deletion in patients with newly diagnosed multiple myeloma (MM). A retrospective analysis was conducted on 411 patients with newly diagnosed MM; among which, 270 received bortezomib-based therapies, and 141 received thalidomide-based therapies. Fluorescence in situ hybridization (FISH) was performed to detect six cytogenetic abnormalities, namely, del(1p32.3), gain(1q21), del(17p13), del(13q14), t(4;14), and t(11;14). Results showed that 8.3% of patients with MM were detected with del(1p32.3) and had significantly more bone marrow plasma cells (P = 0.025), higher ß2-microglobulin levels (P = 0.036), and higher lactate dehydrogenase levels (P = 0.042) than those without del(1p32.3). Univariate analysis showed that patients with del(1p32.3) under thalidomide-based therapies (median PFS 11.6 vs. 31.2 months, P = 0.002; median OS 16.8 vs. 45.9 months, P < 0.001) were strongly associated with short progression-free survival (PFS) (P = 0.002) and overall survival (OS) (P < 0.001). Multivariate analysis revealed that del(1p32.3) remained a powerful independent factor with worse PFS (P = 0.006) and OS (P = 0.016) for patients under thalidomide-based treatments. Patients with del(1p32.3) under bortezomib-based treatments tended to have short PFS and OS. In conclusion, del(1p32.3) is associated with short PFS and OS in patients with MM who received thalidomide- or bortezomib-based treatments.

19.
J Biosci Bioeng ; 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31813671

RESUMO

Recent studies demonstrate that ursolic acid (UA) presents potent antimicrobial activity against a few microorganisms, however, the underlying mechanisms remain elusive. Moreover, little is known about the effect of UA on carbapenem-resistant Enterobacter cloacae (CREC). In this study, we aimed to investigate the mechanisms underlying-antimicrobial activity of UA and its mode of action against CREC. Minimum inhibitory concentrations (MIC) of UA against CREC were determined by the agar dilution method. The antibacterial effect of UA against CREC was elucidated by evaluating changes in intracellular ATP concentration, intracellular pH, membrane potential, membrane integrity, and cell morphology. A crystal violet assay, field emission scanning electron microscopy (FESEM), and confocal laser scanning microscopy (CLSM) were applied to examine the effect of UA on biofilm formation. Furthermore, the inactivation of CREC cells in biofilms was explored by CLSM. Our results showed that UA has a MIC of 0.1 mg/mL against CREC. UA treatment inhibited CREC growth and impaired membrane integrity of CREC cells, as measured by the decrease in intracellular ATP level, pH, and membrane potential, as well as distinctive deformation in cellular morphology. Moreover, UA exerted a significant inhibitory effect on biofilm formation of CREC and inactivated CREC cells within biofilms. These findings reveal that UA has potent antibacterial and antibiofilm activity against CREC and, thus, can be used as a natural food preservative to reduce CREC-related infections.

20.
Molecules ; 24(24)2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31817721

RESUMO

Overexpression of lysine specific demethylase 1 (LSD1) has been found in many cancers. New anticancer drugs targeting LSD1 have been designed. The research on irreversible LSD1 inhibitors has entered the clinical stage, while the research on reversible LSD1 inhibitors has progressed slowly so far. In this study, 41 stilbene derivatives were studied as reversible inhibitors by three-dimensional quantitative structure-activity relationship (3D-QSAR). Comparative molecular field analysis (CoMFA q 2 = 0.623, r 2 = 0.987, r pred 2 = 0.857) and comparative molecular similarity indices analysis (CoMSIA q 2 = 0.728, r 2 = 0.960, r pred 2 = 0.899) were used to establish the model, and the structure-activity relationship of the compounds was explained by the contour maps. The binding site was predicted by two different kinds of software, and the binding modes of the compounds were further explored. A series of key amino acids Val288, Ser289, Gly314, Thr624, Lys661 were found to play a key role in the activity of the compounds. Molecular dynamics (MD) simulations were carried out for compounds 04, 17, 21, and 35, which had different activities. The reasons for the activity differences were explained by the interaction between compounds and LSD1. The binding free energy was calculated by molecular mechanics generalized Born surface area (MM/GBSA). We hope that this research will provide valuable information for the design of new reversible LSD1 inhibitors in the future.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA