Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 4.091
Filtrar
1.
J Mech Behav Biomed Mater ; 101: 103448, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31563846

RESUMO

A thin diamond-like carbon (DLC) film, a graphite-like carbon (GLC) film and a thick diamond-like carbon (PE-DLC) film are deposited on the micro arc oxidation (MAO) coated pure titanium substrates using a hybrid ion beam deposition system, magnetron sputtering and plasma enhanced chemical vapor deposition, respectively. The microstructure, adhesion, biological corrosion resistance and biotribological properties were determined. The results showed that the three duplex coatings presented uneven surface features and increased binding force. The binding force of the duplex coatings was strongly affected by the bonding strength between the MAO coating and Ti substrate. Although the roughness Ra of the three duplex coatings was high, their friction coefficients were small (under 0.22) in the SBF solution. The MAO/DLC and MAO/GLC coatings showed an excellent tribological behavior and corrosion resistance in the SBF solution.

2.
Food Chem ; 305: 125438, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31494498

RESUMO

Fifteen vitisin A-type pyranoanthocyanins (vAPs) were determined in bilberry wines fermented with Saccharomyces cerevisiae and Schizosaccharomyces pombe by HPLC-DAD and UPLC-DAD-ESI-MS/MS. The fermentation involving S. pombe enhanced the production of vAPs compared to the fermentation with pure S. cerevisiae. The formation of vAPs correlated significantly with the decrease in the content of monomeric anthocyanins and pyruvic acid during 12 months of aging. vAPs were more stable than their corresponding monomeric anthocyanins. Methylation in the B-ring and glycosylation with galactose and arabinose further improved the stability of vAPs. Aging for 12 months led to depletion of pyruvic acid and reduction of over 50% of monomeric anthocyanins. The content of vAPs increased by 26-54% during the first six months of aging, followed by a 2.2-10.2% reduction over the following six months. More residual pyruvic acid in S. pombe wines after fermentation consequently enhanced the generation of vAPs during aging.

3.
Waste Manag ; 101: 200-209, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31622865

RESUMO

Present work was focused on recovering gold (Au) from the printed circuit boards (PCBs) of discarded cellphone by bioleaching assisted continuous foam fractionation. First, the cyanide-producing strains of Pseudomonas putida and Bacillus megaterium were co-cultured in order to supply a high cyanide concentration in the nutrient solution for mobilizing Au from waste PCBs (WPCBs). Bioleaching conditions were optimized by using response surface methodology. Under the suitable bioleaching conditions of pH of 10.0, pulp density of 5 g/L and leaching time of 34 h, the Au mobilization percentage was 83.59%. The leaching liquor with an Au concentration of 1.34 mg/L could be used as the feeding solution of continuous foam fractionation after removing solid particles and cell biomass. In order to strengthen foam drainage, a novel internal component of foam fractionation column was developed. Under the suitable operation conditions of CTAB concentration of 0.2 g/L, volumetric air flow rate of 100 mL/min and feed flow rate of 10 mL/min, the enrichment ratio and recovery percentage of Au were 43.62 and 87.46%, respectively. This study is expected to provide an effective strategy to recover Au from WPCBs, and to supplement the depleting natural resources.


Assuntos
Telefone Celular , Resíduo Eletrônico , Cobre , Ouro , Reciclagem
4.
Methods Mol Biol ; 2050: 175-179, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31468492

RESUMO

Electroporation refers to the application of high strength electric pulse to create transient pores in the membrane, thereby enabling the passage of hydrophilic molecules into the cells. Based on the properties of cell and cell wall, the electroporation parameters vary among the algal species. Here, we demonstrated the optimized protocol for successful introduction of recombinant DNA (~5000 bp) into Nannochloropsis oceanica. The linearized recombinant plasmid that harbors eGFP and Bh-sle as the reporter and marker gene, respectively, was electroporated into the electrocompetent N. oceanica cells at voltage of 2200 V, 50 µF, resistance at 600 Ω using electroporator, and the transformed cells were then screened by molecular analysis. The report exemplifies a straightforward and reliable electroporation strategy for generating transgenic N. oceanica cells.

5.
Life Sci ; : 117121, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31794772

RESUMO

AIMS: This study addressed whether endogenous hepatic stem/progenitor (HSP) cells survival were related to the injured signals during liver cirrhosis. MATERIAL AND METHODS: Liver cirrhosis was induced in C57BL/6 mice by administering diethylnitrosamine (DEN) in drinking water. Hematoxylin-eosin staining and Masson's trichrome staining were used to identify infiltrative cells and connective tissues, respectively. The inflammatory activity grade and fibrotic stage, represented as G and S respectively, and evaluated by the International Simplified Grading and Staging System (ISGSS). Endogenous HSP cells (Ng2+HSP) were identified by immunofluorescence staining with an anti-neuro/glial antigen 2 (Ng2) antibody. All data were analyzed using SPSS 22.0 and GraphPad Prism 6 and Student's t-test was performed to determine statistical significance. p-values < 0.05 were considered statistically significant. KEY FINDINGS: All mice administered oral DEN developed liver fibrosis, liver cirrhosis and hepatocellular carcinoma (HCC). During the fibrosis period, observed a positive correlation of survival of endogenous HSP (Ng2+HSP) cells with inflammatory activity. As the disease developed into the cirrhotic stage, when lost correlation of endogenous HSP cells with inflammatory activity, revealed a dramatically reduced survival rate of endogenous HSP (Ng2+HSP) cells. SIGNIFICANCE: The DEN-induced liver cirrhosis could develop into three time zone of fibrosis, cirrhosis and cancer, and the histological patterns in the model are similar to those described in humans. Dramatic survival and less apoptosis of endogenous HSP (Ng2+HSP) cells was within fibrotic state, where inflammation signals is strong, indicating such time zone (1-6 weeks) during liver cirrhosis is important for mobilizing endogenous HSP-based regeneration.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31796390

RESUMO

We present a structure-from-motion (SfM) framework based on a special type of multi-perspective camera called the cross-slit or XSlit camera. Traditional perspective camera based SfM suffers from the scale ambiguity which is inherent to the pinhole camera geometry. In contrast, an XSlit camera captures rays passing through two oblique lines in 3D space and we show such ray geometry directly resolves the scale ambiguity when employed for SfM. To accommodate the XSlit cameras, we develop tailored feature matching, camera pose estimation, triangulation, and bundle adjustment techniques. Specifically, we devise a SIFT feature variant using non-uniform Gaussian kernels to handle the distortions in XSlit images for reliable feature matching. Moreover, we demonstrate that the XSlit camera exhibits ambiguities in pose estimation process which can not be handled by existing work. Consequently, we propose a 14 point algorithm to properly handle the XSlit degeneracy and estimate the relative pose between XSlit cameras from feature correspondences. We further exploit the unique depth-dependent aspect ratio (DDAR) property to improve the bundle adjustment for the XSlit camera. Synthetic and real experiments demonstrate that the proposed XSlit SfM can conduct reliable and high fidelity 3D reconstruction at an absolute scale.

8.
Nat Commun ; 10(1): 5533, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31797924

RESUMO

Diamond is the hardest natural material, but its practical strength is low and its elastic deformability extremely limited. While recent experiments have demonstrated that diamond nanoneedles can sustain exceptionally large elastic tensile strains with high tensile strengths, the size- and orientation-dependence of these properties remains unknown. Here we report maximum achievable tensile strain and strength of diamond nanoneedles with various diameters, oriented in <100>, <110> and <111> -directions, using in situ transmission electron microscopy. We show that reversible elastic deformation depends both on nanoneedle diameter and orientation. <100> -oriented nanoneedles with a diameter of 60 nm exhibit highest elastic tensile strain (13.4%) and tensile strength (125 GPa). These values are comparable with the theoretical elasticity and Griffith strength limits of diamond, respectively. Our experimental data, together with first principles simulations, indicate that maximum achievable elastic strain and strength are primarily determined by surface conditions of the nanoneedles.

9.
Brachytherapy ; 2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31791712

RESUMO

PURPOSE: The purpose of the study was to explore the dose-effect relationship between dose-volume histogram parameters and clinical prognosis of definitive radio(chemo)therapy followed by intracavitary/interstitial brachytherapy in locally advanced cervical cancer. METHODS AND MATERIALS: A retrospective analysis was performed on 110 patients with locally advanced cervical cancer who underwent external beam radiotherapy combined with intracavitary/interstitial brachytherapy with or without chemotherapy from July 2010 to September 2018. We reported D100, D98, and D90 for high-risk clinical target volume (HR-CTV) and intermediate-risk clinical target volume, D2cm³ for organs at risk. Multivariate Cox regression was used to screen independent factors. Dose-volume parameters screened by the Cox regression were incorporated into the probit model for investigating its relationship with survival. RESULTS: The median followup time was 72.33 months. Multivariate Cox regression analysis showed that HR-CTV D100, HR-CTV D98, and HR-CTV D90 were independent factors, affecting the 5-year overall survival (OS), cancer-specific survival (CSS), and local control (LC) rates. The probit model showed that HR-CTV D98 had predictive values for the 5-year OS, CSS, and LC, and HR-CTV D100 had predictive values for the 5-year OS, CSS, whereas HR-CTV D90 had a predictive value only for the 5-year OS. The HR-CTV D98 corresponding to OS ED90, CSS ED90, and LC ED90 was 86.8, 85.6, and 78.6 Gy, respectively. CONCLUSIONS: A significant dependence of OS, CSS, and LC on D98 for HR-CTV was found. When the long-term OS, CSS, and LC rate of the patient was >90%, HR-CTV D98 > 86.8 Gy EQD2, 85.6 Gy EQD2, and 78.6 Gy EQD2 were required.

10.
G3 (Bethesda) ; 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31792007

RESUMO

In order to enrich the Anser cygnoides genome and identify the gene expression profiles of primary and secondary feather follicles development, de novo transcriptome assembly of skin tissues was established by analyzing three developmental stages at embryonic day 14, 18, and 28 (E14, E18, E28). Sequencing output generated 436,730,608 clean reads from nine libraries and de novo assembled into 56,301 unigenes. There were 2,298, 9,423 and 12,559 unigenes showing differential expression in three stages respectively. Furthermore, differentially expressed genes (DEGs) were functionally classified according to genes ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and series-cluster analysis. Relevant specific GO terms such as epithelium development, regulation of keratinocyte proliferation, morphogenesis of an epithelium were identified. In all, 15,144 DEGs were clustered into eight profiles with distinct expression patterns and 2,424 DEGs were assigned to 198 KEGG pathways. Skin development related pathways (mitogen-activated protein kinase signaling pathway, extra-cellular matrix -receptor interaction, Wingless-type signaling pathway) and genes (delta like canonical Notch ligand 1, fibroblast growth factor 2, Snail family transcriptional repressor 2, bone morphogenetic protein 6, polo like kinase 1) were identified, and eight DEGs were selected to verify the reliability of transcriptome results by real-time quantitative PCR. The findings of this study will provide the key insights into the complicated molecular mechanism and breeding techniques underlying the developmental characteristics of skin and feather follicles in Anser cygnoides.

11.
J Am Chem Soc ; 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31793304

RESUMO

Novel actinide cluster fullerenes, U2C2@Ih(7)-C80 and U2C2@D3h(5)-C78, were synthesized and fully characterized by mass spec-trometry, single crystal X-ray crystallography, UV-vis-NIR , Nuclear Magnetic Resonance spectroscopy (NMR), X-ray absorption spectroscopy (XAS), Raman spectroscopy, IR spectroscopy, as well as density functional and multireference wavefunction calcu-lations. The encapsulated U2C2 is the first example of a uranium carbide cluster featuring two U centers bridged by a C≡C unit. The U-C bond distances in these U2C2 clusters are in the range between 2.130-2.421 Å. While the U2C2 cluster in U2C2@C80 adopts a butterfly-shaped geometry with a U-C2-U dihedral angle of 112.7°and a U-U distance of 3.855 Å, the U-U distance in U2C2@C78 is 4.164 Å and the resulting U-C2-U dihedral angle is increased to 149.1°. The combined experimental and quantum-chemical results suggest that the formal U oxidation state is +IV in the U2C2 cluster, and each U center transfers three electrons to the C2n cage and one electron to C2. Different from the strong U=C covalent bonding reported for U2C@C80, the U-C bonds in U2C2 are less covalent and predominantly ionic. The C-C triple bond is somewhat weaker than in HCCH and the C-C π bonds undergo donation bonding with the U centers. This work demonstrates that the combination of the unique encapsulation effect of fullerene cages and the variable oxidation states of actinide elements can lead to the stabilization of novel actinide clusters which are not accessible by conventional synthetic methods.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31802358

RESUMO

Matrix metalloproteinases (MMPs) play a major role in extracellular matrix remodeling and are involved in tumor cell invasion. Cancers such as childhood leukemia are characterized by their capacity to infiltrate different organs. MMP production by leukemic cells may indicate a leukemic subtype or subpopulation with a more invasive phenotype. Therefore, clarifying the action mechanisms of MMPs as prognostic predictors or MMP targeting as a therapeutic strategy is necessary. MMP-targeting drugs have been developed for the treatment of hematological malignancies. In this review, we highlight current advances in understanding the molecular mechanisms and pathological characteristics of various MMPs, as well as recent therapeutic advances targeting MMPs in childhood leukemia. Several studies have been conducted on the therapeutic efficacy of MMP inhibitors in cancer, such as collagen peptidomimetics, nonpeptidomimetic inhibitors of MMP active sites, bisphosphonates, and tetracycline derivatives. Here, we conclude that more clinical trials are necessary to estimate the role of selective MMP inhibitors in the treatment and prevention of childhood leukemia.

13.
Anal Chem ; 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31802668

RESUMO

Glutathione (GSH) levels are closely related to the homeostasis of redox state which directly affects human diseases occurrence by regulating cell apoptosis. Hence, real-time monitoring of dynamic changes in intracellular GSH levels is urgently needed for disease early diagnosis and evaluation of therapy efficiency. In this study, an endogenous cysteine (Cys)-assisted detection system based on GSH@AgNCs and reduced graphene oxide (rGO) with high sensitivity and specificity was developed for GSH detection. Compared with GSH, GSH@AgNCs with weaker affinity and bonding force was quite easier to extrude from the rGO surface when competing against GSH, leading to the obvious change in fluorescence signal. This phenomenon was termed as "a crowding out effect". Furthermore, the presence of Cys can improve GSH assay sensitivity by enhancing the quenching efficiency of rGO on the GSH@AgNCs. In vitro assay indicated that the efficiency of fluorescence recovery was positively related with GSH concentration in the range from 0 to 10 mM. In addition, the method was employed for real-time monitoring of the dynamic changes in GSH levels regulated by natural drugs. The imaging results showed that the natural compound 3 (C3) can downregulate GSH levels in HepG2 cells, which was accompanied by reactive oxygen species (ROS) release and apoptosis induction. Finally, the method was used to monitor the change of GSH levels in serum samples with chronic hepatitis B (CHB) infection. The results demonstrated that the occurrence and development of CHB may be positively correlated with GSH levels to some extent. Overall, the above results demonstrate the potential application of this new nanosystem in anti-cancer natural drug screening and clinical assay regarding GSH levels.

14.
Brain Behav ; : e01444, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31804775

RESUMO

INTRODUCTION: Intermittent fasting (IF) has been suggested to have neuroprotective effects through the activation of multiple signaling pathways. Rodents fasted intermittently exhibit enhanced hippocampal neurogenesis and long-term potentiation (LTP) at hippocampal synapses compared with sedentary animals fed an ad libitum (AL) diet. However, the underlying mechanisms have not been studied. In this study, we evaluated the mechanistic gap in understanding IF-induced neurogenesis. METHODS: We evaluated the impact of 3 months of IF (12, 16, and 24 hr of food deprivation on a daily basis) on hippocampal neurogenesis in C57BL/6NTac mice using immunoblot analysis. RESULTS: Three-month IF significantly increased activation of the Notch signaling pathway (Notch 1, NICD1, and HES5), neurotrophic factor BDNF, and downstream cellular transcription factor, cAMP response element-binding protein (p-CREB). The expression of postsynaptic marker, PSD95, and neuronal stem cell marker, Nestin, was also increased in the hippocampus in response to 3-month IF. CONCLUSIONS: These findings suggest that IF may increase hippocampal neurogenesis involving the Notch 1 pathway.

15.
Curr Opin Struct Biol ; 61: 25-32, 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31783299

RESUMO

Helicases are ATP-dependent motor proteins that translocate along single-stranded or double-stranded nucleic acids to alter base-pairing structures or molecular interactions. Helicases can be divided to monomeric and hexameric types, each with distinct ternary structures, nucleic acid-binding modes, and translocation mechanisms. It is well established that monomeric helicases translocate by the inchworm mechanism. Recent structures of different superfamilies of hexameric helicases reveal that they use a hand-over hand mechanism for translocation. Structures of bacteriophage T7 replisome illustrate how helicase and polymerase cooperatively catalyze DNA unwinding. In this review, we survey structures of monomeric and hexameric helicases and compare different mechanisms for translocation.

16.
Eur J Pharmacol ; : 172775, 2019 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-31689413

RESUMO

Individuals with traumatic brain injury (TBI) manifest a high incidence of depression, which is associated with an impaired recovery from TBI and a lower quality of life. Several neurobiological changes in patients with TBI contribute a form of depression that is unique to that of general depression. This is evinced by the poor efficacy of antidepressants in treating post-TBI depression relative to general depression. In general, however, the treatment of post-TBI depression has received relatively scattered attention in the literature. The purpose of this review is thus to discuss about the possible pathology of depression following TBI and summarize the recent findings on the treatment of it in clinical studies. While both pharmacological and non-pharmacological approaches can reportedly attenuate depressive symptoms in patients with TBI to a moderate extent, the various limitations of such studies require that further well-powered, randomized controlled trials with larger sample sizes and longer follow-ups are warranted to investigate the exact pathophysiology underlying post-TBI depression, the mechanism underlying treatment efficacy, and the optimal pharmacological and non-pharmacological interventions for this population. A combination of different treatments in a comprehensive therapeutic regimen may be an optimal direction for future research.

17.
J Alzheimers Dis ; 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31683484

RESUMO

Exaggerated Ca2+ signaling might be one of primary causes of neural dysfunction in Alzheimer's disease (AD). And the intracellular Ca2+ overload has been closely associated with amyloid-ß (Aß)-induced endoplasmic reticulum (ER) stress and memory impairments in AD. Here we showed for the first time the neuroprotective effects of Xestospongin C (XeC), a reversible IP3 receptor antagonist, on the cognitive behaviors and pathology of APP/PS1 AD mice. Male APP/PS1-AD mice (n = 20) were injected intracerebroventricularly with XeC (3µmol) via Alzet osmotic pumps for four weeks, followed by cognition tests, Aß plaque examination, and ER stress-related protein measurement. The results showed that XeC pretreatment significantly improved the cognitive behavior of APP/PS1-AD mice, raising the spontaneous alteration accuracy in Y maze, decreasing the escape latency and increasing the target quadrant swimming time in Morris water maze; XeC pretreatment also reduced the number of Aß plaques and the overexpression of ER stress proteins 78 kDa glucose-regulated protein (GRP-78), caspase-12, and CAAT/enhancer-binding protein (C/EBP) homologous protein (CHOP) in the hippocampus of APP/PS1 mice. In addition, in vitro experiments showed that XeC effectively ameliorated Aß1 - 42-induced early neuronal apoptosis and intracellular Ca2+ overload in the primary hippocampal neurons. Taken together, IP3R-mediated Ca2+ disorder plays a key role in the cognitive deficits and pathological damages in AD mice. By targeting the IP3 R, XeC might be considered as a novel therapeutic strategy in AD.

18.
Plast Reconstr Surg ; 144(6): 1417-1428, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31764662

RESUMO

BACKGROUND: Computer-assisted mandibular reconstruction facilitates preoperative surgery simulation and transfers the virtual plan to a real operation. This systematic review and meta-analysis aimed to compare the accuracy, efficiency, postoperative complications, and economic viability between computer-assisted mandibular reconstruction and conventional freehand mandibular reconstruction. METHODS: The PubMed, Embase, Cochrane Library, and Google Scholar databases were searched up to November of 2018. The accuracy, efficiency, postoperative complications, and economic viability of computer-assisted mandibular reconstruction compared to conventional freehand mandibular reconstruction were systematically reviewed. Continuous and dichotomous data were pooled in mean difference (or standardized mean difference if necessary) and odds ratio, subsequently, with 95 percent confidence interval. RESULTS: A total of 12 studies were included in the systematic review, and data extracted from 11 of them were combined in meta-analysis. The accuracy of computer-assisted mandibular reconstruction was better than or equal to that of conventional freehand mandibular reconstruction according to qualitative analysis, although the quantitative comparison from meta-analysis was excluded because of the diversity of measurements. As for efficiency, computer-assisted mandibular reconstruction, when compared to conventional freehand mandibular reconstruction, revealed a shorter ischemic time, reconstructive time, total operative time, and length of stay. There was no difference in postoperative complication rate. CONCLUSIONS: Computer-assisted mandibular reconstruction showed increased efficiency considering the reduced ischemic time, total operative time, reconstructive time, and length of stay. However, the accuracy, reconstruction outcomes, and perioperative cost should be further elucidated because of diverse measurements and the lack of included studies.

19.
Nature ; 574(7780): 653-657, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31666722

RESUMO

Superconductivity can occur under conditions approaching broken-symmetry parent states1. In bilayer graphene, the twisting of one layer with respect to the other at 'magic' twist angles of around 1 degree leads to the emergence of ultra-flat moiré superlattice minibands. Such bands are a rich and highly tunable source of strong-correlation physics2-5, notably superconductivity, which emerges close to interaction-induced insulating states6,7. Here we report the fabrication of magic-angle twisted bilayer graphene devices with highly uniform twist angles. The reduction in twist-angle disorder reveals the presence of insulating states at all integer occupancies of the fourfold spin-valley degenerate flat conduction and valence bands-that is, at moiré band filling factors ν = 0, ±1, ±2, ±3. At ν ≈ -2, superconductivity is observed below critical temperatures of up to 3 kelvin. We also observe three new superconducting domes at much lower temperatures, close to the ν = 0 and ν = ±1 insulating states. Notably, at ν = ± 1 we find states with non-zero Chern numbers. For ν = -1 the insulating state exhibits a sharp hysteretic resistance enhancement when a perpendicular magnetic field greater than 3.6 tesla is applied, which is consistent with a field-driven phase transition. Our study shows that broken-symmetry states, interaction-driven insulators, orbital magnets, states with non-zero Chern numbers and superconducting domes occur frequently across a wide range of moiré flat band fillings, including close to charge neutrality. This study provides a more detailed view of the phenomenology of magic-angle twisted bilayer graphene, adding to our evolving understanding of its emergent properties.

20.
Soc Sci Med ; 244: 112667, 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31734601

RESUMO

China piloted a publicly funded Long-term Care Nursing Insurance (LTCNI) with an aim to improve access to long-term care (LTC) for older people in China in 2012. Existing studies showed that the scheme has been successful in meeting some goals, but little is known on how the availability, price and quality of the local care providers affect access to care. Using an Agent-based Modelling approach, this paper simulates the effects of the LTCNI on access to care by considering quality, costs and availability of the local care providers. Our simulation is based on Qingdao city-the first city in China that piloted the LTCNI. We found considerable discrepancies in terms of access to services among the LTCNI beneficiaries. The poor are more likely to choose nursing home care with low costs albeit these services may be of poor quality. The demand for home-based services is high, but these services are not distributed according to population needs and are not available in some districts with high concentration of older people. Consequently, a large proportion of disabled older people have no access to home-based services. We urge policy makers to improve the benefit package of the LTCNI, especially for the poor. We also suggest that more resources should be directed to promote and improve the availability of home-based services in areas with a large number of older people.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA