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1.
Prenat Diagn ; 2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-33000500

RESUMO

OBJECTIVE: To explore the copy number variants (CNVs) in case of fetal duodenal obstruction and assess the associated prenatal findings and postnatal outcomes. MATERIALS AND METHODS: This retrospective study reviewed 51 fetuses with duodenal obstruction (DO) and the findings of chromosomal microarray analysis (CMA) used as a first-tier test in our institution between January 2014 and May 2019. RESULTS: The frequency of pathogenic aberrations in fetuses with DO was 15.7% (8/51), including 9.8% (5/51) pathogenic CNVs. Three fetuses with isolated DO each had a deletion on chromosome 13q, one fetus had duplication at 1q43q44, and one had microduplication at 17q12. No significant differences in pathogenic CNVs were observed between isolated DO and DO plus additional anomalies (4/42, 9.5% vs 1/9, 11.1%, p=0.89). Of the 51 fetuses with DO, 11 pregnancies were terminated, and eight fetuses had chromosomal abnormalities; one pregnancy ended with intrauterine death, and there were 39 live births. Neonatal outcomes were available for 31 fetuses, and no neonatal deaths occurred after surgery. CONCLUSIONS: Our cohort study demonstrated the value of CMA in fetuses with DO, suggesting that CNVs may underly genetic etiologies that should be considered in the diagnostic evaluation of DO. We think CMA should be recommended in case of DO. This article is protected by copyright. All rights reserved.

2.
Adv Mater ; : e2003592, 2020 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-33015911

RESUMO

Low-cost and high-safety aqueous Zn-ion batteries are an exceptionally compelling technology for grid-scale energy storage. However, their development has been plagued by the lack of stable cathode materials allowing fast Zn2+ -ion insertion and scalable synthesis. Here, a lattice-water-rich, inorganic-open-framework (IOF) phosphovanadate cathode, which is mass-producible and delivers high capacity (228 mAh g-1 ) and energy density (193.8 Wh kg-1 or 513 Wh L-1 ), is reported. The abundant lattice waters functioning as a "charge shield" enable a low Zn2+ -migration energy barrier, (0.66 eV) even close to that of Li+ within LiFePO4 . This fast intrinsic ion-diffusion kinetics, together with nanostructure effect, allow the achievements of ultrafast charging (71% state of charge in 1.9 min) and an ultrahigh power density (7200 W kg-1 at 107 Wh kg-1 ). Equally important, the IOF exhibits a quasi-zero-strain feature (<1% lattice change upon (de)zincation), which ensures ultrahigh cycling durability (3000 cycles) and Coulombic efficiencies of 100%. The cell-level energy and power densities reach ≈90 Wh kg-1 and ≈3320 W kg-1 , far surpassing commercial lead-acid, Ni-Cd, and Ni-MH batteries. Lattice-water-rich IOFs may open up new opportunities for exploring stable and fast-charging Zn-ion batteries.

3.
J Diabetes Investig ; 2020 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-33068491

RESUMO

AIMS/INTRODUCTION: Type 2 diabetes mellitus(T2DM) iss, though which FFA is most associated with T2DM is unclear. This study aimed to identify the specific FFAs that best predict the occurrence of T2DM during obesity and assess their potential application value. MATERIALS AND METHODS: Subjects were divided into three groups: a normal weight (NW) group (n=20), an obese (OB) group (n=10) and a T2DM group (n=10). FFAs in serum samples were determined by ultra-high-pressure liquid chromatography-mass spectrometry (UHPLC - MS) and orthogonal partial least squares discriminant analysis (OPLS-DA) models were used to study the FFA profile among the three groups. RESULTS: Compared with the NW group, 14 FFAs(C8:0/10:0/14:0/16:1/18:1/20:2/ 20:3 /20:4/ 20:5/ 22:6/7:0/9:0/11:0 and C13:0) were significantly increased in the OB group, and 9 FFAs (C14:0/18:1/20:1/18:2/20:2/20:3/18:3/20:5 and C22:6) were significantly increased in the T2DM group. Subsequently, the Venn diagram results showed that 6 FFAs (C14:0/18:1/20:2/20:3/20:5 and C22:6) were significantly increased in both the OB and T2DM groups. Among these six, C22:6 was finally identified as an independent risk factor for T2DM and had a great potential to predict the susceptibility to T2DM (AUC=0.803). CONCLUSIONS: C22:6 can be an independent risk factor for T2DM, and it has a great potential to predict the susceptibility of T2DM.

4.
Mol Ecol ; 2020 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-33006793

RESUMO

Genetic stochasticity and bottlenecking in the course of Pleistocene glaciations have been identified as threatening the survival of local endemics. However, the mechanisms by which local endemic species balance the influences of these two events remain poorly understood. Here, we generated a double-digest restriction site-associated DNA sequencing (ddRAD-seq) data set, mined mitochondrial sequences and constructed ecological niche models for the island endemic water strider Metrocoris esakii (Hemiptera: Gerridae). We found that M. esakii comprised three divergent lineages (i.e., north, central and south) isolated by geographical barriers and generally experienced population declines with the constriction of suitable areas during the Last Glacial Maximum (LGM). Further demographic model testing and stairway plots revealed a history of recent gene flow among the neighbouring lineages and rapid recovery at the end of the LGM, indicating that M. esakii at least had the potential for an adaptive response to population fragmentation and bottlenecking. The northern lineage did not show genetic bottlenecking during the LGM, which was probably due to its large effective population size (Ne ) from migration, which improved its adaptive potential. Relative to the ddRAD-seq data set, the demographic results based on mitochondrial sequences were less conclusive, showing weak differentiation and oversimplified demographic trajectories for the three genetic lineages. Overall, this study provides some degree of optimism for the survival of island endemic water striders from a demographic perspective, but further evaluation of their extinction risk under the impacts of human activities is required.

5.
Artigo em Inglês | MEDLINE | ID: mdl-33049148

RESUMO

Diabetic nephropathy (DN) is a common and complicated chronic kidney disease around the world. To elucidate and find effective therapies of DN is of vital importance. In this paper, we have discovered that Cyanidin-3-O-glucoside (C3G), which is one of the anthocyanins, could alleviate high glucose induced podocytes dysfunction. MTT, flow cytometry assay and western blot analysis showed that C3G could reverse the increase of cell apoptosis under high glucose treatment in MPC5 cells by up-regulation of Bcl2 and down-regulation of Bax and cleaved-caspase 3. Moreover, C3G improved the autophagy decrease that was induced by high glucose through regulating the expression level of LC3 II/LC3 I, Beclin1 and p62. In addition, C3G inhibited epithelial-mesenchymal transition (EMT) by increasing E-cadherin and reducing Vimentin. By further mechanisms study, we found C3G activated the SIRT1 and AMPK which were inhibited in high glucose condition. Silencing SIRT1 blocked the effect of C3G on regulating cell apoptosis, autophagy and EMT. In summary, our current findings suggest the protective effect of C3G against high glucose induced podocytes dysfunction is by improving autophagy and reducing apoptosis and EMT via activating SIRT1/AMPK pathway. It might be a new insight for the treatment of DN.

6.
Int J Mol Sci ; 21(19)2020 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-33050099

RESUMO

Nitrogen (N), phosphorus (P), and potassium (K) are three essential macro-elements for plant growth and development. Used to improve yield in agricultural production, the excessive use of chemical fertilizers often leads to increased production costs and ecological environmental pollution. Vitamins C and E are antioxidants that play an important role in alleviating abiotic stress. However, there are few studies on alleviating oxidative stress caused by macro-element deficiency. Here, we used Arabidopsis vitamin E synthesis-deficient mutant vte4 and vitamin C synthesis-deficient mutant vtc1 on which exogenous vitamin E and vitamin C, respectively, were applied at the bolting stage. In the deficiency of macro-elements, the Arabidopsis chlorophyll content decreased, malondialdehyde (MDA) content and relative electric conductivity increased, and reactive oxygen species (ROS) accumulated. The mutants vtc1 and vte4 are more severely stressed than the wild-type plants. Adding exogenous vitamin E was found to better alleviate stress than adding vitamin C. Vitamin C barely affected and vitamin E significantly inhibited the synthesis of ethylene (ETH) and jasmonic acid (JA) genes, thereby reducing the accumulation of ETH and JA that alleviated the senescence caused by macro-element deficiency at the later stage of bolting in Arabidopsis. A deficiency of macro-elements also reduced the yield and germination rate of the seeds, which were more apparent in vtc1 and vte4, and adding exogenous vitamin C and vitamin E, respectively, could restore them. This study reported, for the first time, that vitamin E is better than vitamin C in delaying seedling senescence caused by macro-element deficiency in Arabidopsis.

7.
Nat Commun ; 11(1): 4909, 2020 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-32999291

RESUMO

Effectively activating macrophages against cancer is promising but challenging. In particular, cancer cells express CD47, a 'don't eat me' signal that interacts with signal regulatory protein alpha (SIRPα) on macrophages to prevent phagocytosis. Also, cancer cells secrete stimulating factors, which polarize tumor-associated macrophages from an antitumor M1 phenotype to a tumorigenic M2 phenotype. Here, we report that hybrid cell membrane nanovesicles (known as hNVs) displaying SIRPα variants with significantly increased affinity to CD47 and containing M2-to-M1 repolarization signals can disable both mechanisms. The hNVs block CD47-SIRPα signaling axis while promoting M2-to-M1 repolarization within tumor microenvironment, significantly preventing both local recurrence and distant metastasis in malignant melanoma models. Furthermore, by loading a stimulator of interferon genes (STING) agonist, hNVs lead to potent tumor inhibition in a poorly immunogenic triple negative breast cancer model. hNVs are safe, stable, drug loadable, and suitable for genetic editing. These properties, combined with the capabilities inherited from source cells, make hNVs an attractive immunotherapy.


Assuntos
Micropartículas Derivadas de Células/imunologia , Imunoterapia/métodos , Macrófagos/imunologia , Melanoma/terapia , Recidiva Local de Neoplasia/prevenção & controle , Neoplasias de Mama Triplo Negativas/terapia , Animais , Antígeno CD47/metabolismo , Linhagem Celular Tumoral/transplante , Modelos Animais de Doenças , Feminino , Células HEK293 , Humanos , Ativação de Macrófagos/efeitos dos fármacos , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Melanoma/imunologia , Melanoma/secundário , Proteínas de Membrana/agonistas , Proteínas de Membrana/imunologia , Camundongos , Nanopartículas/administração & dosagem , Recidiva Local de Neoplasia/imunologia , Nucleotídeos Cíclicos/administração & dosagem , Receptores Imunológicos/metabolismo , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/imunologia , Neoplasias de Mama Triplo Negativas/imunologia , Evasão Tumoral/efeitos dos fármacos , Evasão Tumoral/imunologia , Microambiente Tumoral/imunologia
8.
J Colloid Interface Sci ; 583: 234-242, 2020 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-33002695

RESUMO

Heating treatment is widely used in the preparation of metallic materials with controlled phase behavior and mechanical properties. However, for the soft materials assembled by short peptides, especially simple dipeptides, the detailed influences of heating treatment on the structures and functions of the materials remain largely unexplored. Here we showed that by thermal annealing or quenching of aromatic peptide solutions under kinetic control, we are able to control the self-assembly of peptide into materials with distinct phase behavior and macroscopic properties. The thermal annealing of the heated peptide solutions will lead to the formation of large nanobelts or bundles in solution, and no gels will be formed. However, by quenching the heated peptide solution, a self-supporting hydrogel will be formed quickly. Structure analysis revealed that the peptides preferred to self-assembled into much thinner and flexible nanohelices during quenching treatment. Moreover, the stability of the gels further increased with the repeated heating and quenching cycling of the peptide solutions. The results demonstrated that the heat treatment can be used to control the structure and function of self-assembled materials in a way similar to that of the conventional metallic or alloy materials.

9.
Eur J Radiol ; 132: 109286, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-33007519

RESUMO

PURPOSE: This study aims to compare pelvic incidence (PI), a skeletal angle formed by the first sacral vertebrae and femoral heads, in women with and without pelvic organ prolapse (POP) and to explore the correlation of PI with the progression of POP, through 3D reconstruction of MRI scans. METHOD: The case-control study enrolled 48 prolapse patients and 48 paired subjects by collecting and screening clinical information including age, BMI, vaginal deliveries, and levator ani defect scores. PI values were measured in 3D reconstruction models based on MRI scans, and the mean and standard deviation values of PI in both groups were calculated. Receiver operating characteristic (ROC) analysis and logistic regression were used to quantify relationships between PI and prolapse. Additionally, by performing a cross-section study of 69 patients with POP, correlations between PI values and descending vaginal locations were assessed by multivariate linear regression models. RESULTS: Compared with the control group, the patient group has a significantly larger average PI (48.68 ±â€¯10.77˚ vs 42.20 ±â€¯8.55˚, P=0.002). ROC analysis for the classification of prolapse based on PI has an area under the curve of 70.1 % (P < 0.001). Logistic regression identified a larger PI value as a risk factor of prolapse with an odds ratio of 2.90 (95 %CI: 1.46-5.74, P = 0.002) for PI per increasing 10˚. Point Ba and Bp represent the most distal positions of any part of the upper anterior and posterior vaginal walls, respectively. In the patient group, internally, Ba and Bp would descend 0.62 (95 %CI: 0.24-1.00, P=0.002) cm and 0.74 (95 %CI 0.22-1.26, P=0.006) cm for every 10° increase in PI, respectively. The coefficients of the partial correlation of PI for Ba and Bp are 0.381 (P = 0.002) and 0.336 (P = 0.006). CONCLUSIONS: PI is significantly related to morbidity and progression of POP, especially for the anterior and posterior pelvic compartments. As an individual constant value of the spinopelvic skeletal shape, a larger PI value is a risk factor and should be evaluated carefully in medical imaging of POP.

10.
Artigo em Inglês | MEDLINE | ID: mdl-33066542

RESUMO

Based on interviews with 695 smallholders in Jianghan Plain, this paper introduced the three-stage data envelopment analysis (DEA) model to analyze the agricultural production efficiency of conservation tillage adopters and explored the impact of environmental factors on agricultural production efficiency. The empirical results showed the following (1) Planting area, seed consumption, labor input, pesticide usage, chemical fertilizer usage, agricultural film usage were selected as input indicators, agricultural output was chosen as an output indicator, and the traditional DEA model was used to calculate the production efficiency of smallholders, and the agricultural production efficiency of smallholders was found to be at a low level. In addition, environmental and random factors both have significant impacts on efficiency, so they should be stripped. (2) After excluding environmental factors and random factors, the drop in pure technical efficiency of smallholders in the third stage was higher than the drop in scale efficiency when compared with the first stage. Moreover, the true technical efficiency was the main restricting factor for the agricultural production efficiency. (3) Educational level of smallholders, policy support, and information acquisition were the factors that affect the technical efficiency significantly. Improving the efficiency of agricultural production technology for smallholders requires strengthening rural basic education, improving subsidy policies for conservation agricultural technology, and establishing and improving rural information technology services.

11.
Front Immunol ; 11: 2188, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33072082

RESUMO

The understanding of protective immunity during HIV infection remains elusive. Here we showed that CD160 defines a polyfunctional and proliferative CD8+ T cell subset with a protective role during chronic HIV-1 infection. CD160+ CD8+ T cells derived from HIV+ patients correlated with slow progressions both in a cross-sectional study and in a 60-month longitudinal cohort, displaying enhanced cytotoxicity and proliferative capacity in response to HIV Gag stimulation; triggering CD160 promoted their functionalities through MEK-ERK and PI3K-AKT pathways. These observations were corroborated by studying chronic lymphocytic choriomeningitis virus (LCMV) infection in mice. The genetic ablation of CD160 severely impaired LCMV-specific CD8+ T cell functionalities and thereby resulted in loss of virus control. Interestingly, transcriptional profiling showed multiple costimulatory and survival pathways likely to be involved in CD160+ T cell development. Our data demonstrated that CD160 acts as a costimulatory molecule positively regulating CD8+ T cells during chronic viral infections, thus representing a potential target for immune intervention.

12.
Appl Opt ; 59(26): 7961-7967, 2020 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-32976471

RESUMO

Direct reconstruction of the noncontact time-domain fluorescence molecular lifetime tomography (TD-FMLT) with current nonlinear algorithms has suffered from complexity and heavy computation loads of the physical model for a large imaging area in TD-FMLT. In this work, we discretize the system matrix along time points and apply a linearized reconstruction algorithm using the fused least absolute shrinkage and selection operator method. The reconstructed yield map and object geometry are used as a priori information to mitigate the ill conditions. This approach is implemented on a fully noncontact TD-FMLT system equipped with a femtosecond pulse laser and a high-speed, time-gated camera. We validate the methodology using both numerical simulations and inhomogeneous phantom experiments. The results exhibit good localization accuracy for fluorescent targets and an efficient computation capability for the reconstruction of fluorescence lifetime in noncontact TD-FMLT. We envision that the proposed linear scheme for the direct reconstruction method in noncontact TD-FMLT has a significant potential for in vivo preclinical studies.

13.
Zhongguo Zhong Yao Za Zhi ; 45(15): 3681-3688, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-32893558

RESUMO

This study aims to explore the change laws of water absorption in Chinese herbal pieces and establish the prediction model of relative density for Chinese medicine compound decoction. Firstly, fitted equations of water absorption and decocting time was established by observing the change laws of water absorption in 36 kinds of Chinese herbal pieces in 12 groups(according to the drug-parts) with decocting time. The r value of the mineral group and other type group was 0.691 2 and 0.663 3, respectively. The r value of the remaining 10 groups was 0.802 2-0.925 4. All P values were less than 0.05(n=21). The formula of the amount of water added was optimized by combining the fitted equations with determined water absorption, and the liquid yield could be controlled in a range of 100%±10%. Secondly, it was determined that the liquid density tester could be used for the rapid determination of relative density of Chinese medicine decoction after methodological study and comparison with the pycnometer method. The linear regression equation between the corrected relative density(y) and extraction ratio(%, x) was built by measuring and analyzing the related parameters such as liquid yield, relative density and extraction ratio in 46 kinds of Chinese herbal pieces. The established equation was y=0.041 3x+1.003 7, r=0.930 9(P <0.01, n=46), with linear range of 1.94%-65.75%. Based on this, the prototype model for predicting relative density of Chinese medicine decoction was established, and the relative densities of 8 Chinese medicine decoctions were within the prediction interval of this model in verification. This study lays a foundation for database construction of Chinese medicine decoction, implementation of personalized decocting mode and rapid quality control of Chinese medicine decoction.


Assuntos
Medicamentos de Ervas Chinesas , Medicina Tradicional Chinesa , Controle de Qualidade , Gravidade Específica , Água
14.
Int J Nanomedicine ; 15: 6827-6838, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32982235

RESUMO

The ultimate goal of phototherapy based on nanoparticles, such as photothermal therapy (PTT) which generates heat and photodynamic therapy (PDT) which not only generates reactive oxygen species (ROS) but also induces a variety of anti-tumor immunity, is to kill tumors. In addition, due to strong efficacy in clinical treatment with minimal invasion and negligible side effects, it has received extensive attention and research in recent years. In this paper, the generations of nanomaterials in PTT and PDT are described separately. In clinical application, according to the different combination pathway of nanoparticles, it can be used to treat different diseases such as tumors, melanoma, rheumatoid and so on. In this paper, the mechanism of pathological treatment is described in detail in terms of inducing apoptosis of cancer cells by ROS produced by PDT, immunogenic cell death to provoke the maturation of dendritic cells, which in turn activate production of CD4+ T cells, CD8+T cells and memory T cells, as well as inhibiting heat shock protein (HSPs), STAT3 signal pathway and so on.

15.
ACS Appl Mater Interfaces ; 12(39): 43488-43500, 2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-32870657

RESUMO

Self-assembling natural small molecules (NSMs) with favorable anticancer activity are of increasing interest as novel drug delivery platforms without structural modification for biomedical applications. However, a lack of knowledge and practicability of NSMs as drug carriers limited their current biomedical application. Here, via a green and facile supramolecular coassembly strategy, we report and develop a series of carrier-free terpenoid natural small molecule-mediated coassembled photosensitive drugs for enhanced and synergistic chemo/photodynamic therapy. After screening 17 terpenoid NSMs, we identified 11 compounds that could form coassembled NSMs-Ce6 NPs with regulatable drug sizes. Analysis of the representative betulonic acid (BC)-mediated nano-coassemblies (BC-Ce6 NPs) reveals the high efficiency of the coassembly strategy and highlights the tremendous potential of NSMs as novel drug delivery platforms. Through molecular dynamics simulation and theoretical calculations, we elucidate the mystery of the coassembly process, indicating that the linear coplanar arrangement of BC dimeric units is primarily responsible for the formation of rod-like or spherical morphology. Meanwhile, we demonstrated that the reduced energy gap between the singlet and triplet excited states (ΔEST) facilitates efficient reactive oxygen species generation by promoting ·OH generation via a type I photoreaction mechanism. The assembled nanodrugs exhibit multiple favorable therapeutic features, ensuring a remarkably enhanced, synergistic, and secure combinatorial anticancer efficacy of 93.6% with highly efficient tumor ablation. This work not only expands the possibility of natural biodegradable materials for wide biological applications but also provides a new perspective for the construction of NSM-mediated nano-coassemblies for precision therapy.

16.
Transplantation ; 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32890136

RESUMO

BACKGROUND: Hepatic steatosis is a major risk factor for graft failure due to increased susceptibility of fatty liver to ischemia-reperfusion injury (IRI) during transplantation. Here, we aimed to investigate the role of carnitine palmitoyltransferase 1A (CPT1A) in fatty liver graft injury and to explore the underlying mechanism and therapeutic potential on attenuating hepatic IRI. METHODS: Intragraft CPT1A expression profile and the association with fatty graft injury were investigated in human and rat liver transplantation samples. The underlying mechanism and therapeutic potential of CPT1A activator against IRI were also explored in mouse hepatic IR plus major hepatectomy model and in vitro. RESULTS: CPT1A expression was significantly reduced (p = 0.0019; n = 96) in human fatty liver graft compared with normal one at early phase after transplantation. Low expression of CPT1A was significantly associated with high serum alanine aminotransferase (p = 0.0144) and aspartate aminotransferase (p = 0.0060) levels. The inhibited CPT1A and poor liver function were consistently observed in rat and mouse models with fatty livers. Furthermore, inhibition of CPT1A significantly promoted the translocation of chloride intracellular channel 1 to form chloride ion channel. The dysregulation of chloride ion channel activity subsequently triggered mitochondrial permeability transition pore opening, exacerbated cellular oxidative stress and energy depletion. Importantly, our intravital confocal imaging showed that CPT1A activation attenuated hepatic injury through preventing mitochondrial permeability transition (MPT) after reperfusion in fatty mice. CONCLUSIONS: CPT1A inhibition triggered MPT contributed to severe IRI in fatty liver graft. CPT1A restoration may offer therapeutic potential on attenuating hepatic IRI.

17.
J Sex Med ; 2020 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-32868262

RESUMO

BACKGROUND: Gender-affirming hormone therapy and surgery are important medically necessary approaches to transgender care. However, few related data exist in China. AIM: To understand the desire and access of transgender cares in the Chinese transgender men and women population. METHODS: A cross-sectional self-selecting survey targeting the Chinese transgender population was conducted in 2017 using a snowball sampling method. Participants completed an online questionnaire anonymously. Gender identity was verified by specifically designed questions. Data analysis of this study was performed in 2019. OUTCOMES: The main outcome was the status of receiving transgender medical care, including the desire vs actual state of receiving gender-affirming hormone treatment and gender-affirmation surgery, methods of accessing hormonal therapy and surgery, and risky behaviors associated with obtaining treatments. RESULTS: Of the total 2060 valid questionnaires, there were 1,304 transgender individuals (626 transgender men and 678 transgender women), with a median age of 22 (interquartile range, 19-26) years. Among them, 1,036 (79.4%) expressed desires for hormonal therapy, but of 1,036, 741 (71.5%) considered it difficult to obtain medications from doctors. Of 1,036 individuals, 275 (26.5%) and 172 (16.6%) had thoughts or behaviors of self-injury, respectively, when lacking access to hormone therapy. Of 1,036 individuals, 602 (58.1%) had used hormones. Of those 602 hormone users, 407 (67.6%) had ever obtained medications from informal drug dealers, and 372 (61.8%) of them did not perform regular monitoring. 868 of 1,303 (66.6%) participants had received or wanted to undergo gender-affirming surgeries, but 710 of 868 (81.8%) considered the surgery resources not adequate or very scarce. CLINICAL IMPLICATIONS: The transgender medical resources in China are scarce, and many transgender individuals have engaged in high-risk activities to access care. STRENGTHS & LIMITATIONS: This is the first study to focus on the current status of gender-affirming hormone therapy and surgery in the Chinese transgender population, providing valuable and real-world data for understanding the need for transgender health care in China. But, the online questionnaire could not provide the prevalence and other epidemiologic information about transgender individuals in China, and the survey did not address specific medication regimens, dosages, sex hormone levels, and specific hormone therapy-related or surgery-related adverse events. CONCLUSION: Significant improvement in access to gender-affirming medical and surgery care is needed in China. Liu Y, Xin Y, Qi J, et al. The Desire and Status of Gender-Affirming Hormone Therapy and Surgery in Transgender Men and Women in China: A National Population Study. J Sex Med 2020;XX:XXX-XXX.

18.
Sci Total Environ ; 750: 141646, 2020 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-32866748

RESUMO

Effectively balancing soil moisture and biodiversity restoration remains a contentious issue for managers and researchers in the Loess Plateau region of China, even after many years of restoration efforts. We conducted a regional study on the trade-off between soil moisture and species diversity using spatial grid sampling in a semi-arid steppe (200-300 mm annual precipitation) in the northwest Loess Plateau. Results reveal that only soil moisture between 20 and 60 cm depth was significantly correlated with diversity indexes. Root-mean-square deviation (RSMD, the index of the soil water-biodiversity relationship) increased by monotonous linear trends with soil moisture in 20-60 cm depth. The linear relationship for Shannon Wiener diversity index (SD) was stronger than for species richness index (SR). When soil moisture in 20-60 cm depth was lower than 6-8%, RSMD often was less than zero, representing the trade-off relationship. However, synergism was more common as the soil moisture increased beyond 6-8%. The overall trends and the soil moisture threshold (6-8%) did not differ significantly between sites with different vegetation cover and aspect, though there were differences in the relative ratio of trade-off and synergism samples. Comparing results from sampling at different scales in the Loess Plateau suggests 6-8% soil moisture in 200-300 mm precipitation gradient, consistent with 370 mm rainfall depth in 250-550 mm precipitation gradient, might be a scale-independent threshold driving the soil moisture-biodiversity relationship from trade-off to synergism in the region.

19.
Mol Cell Biochem ; 2020 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-32970285

RESUMO

Circular RNA zinc finger protein 609 (circZNF609) has been reported to involve in nasopharyngeal carcinoma (NPC) tumorigenesis regulation. However, the role and the molecular mechanism of circZNF609 in NPC remain unclear. Levels of circZNF609, microRNA (miR)-338-3p, and GTPase HRas (HRAS) were detected by quantitative real-time polymerase chain reaction or Western blot. Cell proliferation, migration, and invasion were analyzed using cell counting kit-8 assay, colony formation assay, and transwell assay, respectively. Glucose metabolism was calculated by measuring glucose consumption, lactate production, adenosine triphosphate (ATP) levels, and HK2 activity. The interaction between miR-338-3p and circZNF609 or HRAS was analyzed by the dual-luciferase reporter assay. In vivo experiment was conducted using the murine xenograft model. CircZNF609 was elevated in NPC tissues and cell lines, and high circZNF609 expression had a poor prognosis. CircZNF609 knockdown suppressed NPC progression in vitro by inhibiting cell proliferation, migration, invasion, and glycolysis and hindered tumor growth in vivo. MiR-338-3p directly bound to circZNF609 and HRAS, and circZNF609 knockdown repressed NPC cell malignant properties by binding to miR-338-3p. MiR-338-3p was low in NPC, and miR-338-3p restoration performed anti-tumor effects in cells of NPC by targeting HRAS. Importantly, circZNF609 acted as a competing endogenous RNA of miR-338-3p to regulate HRAS. CircZNF609 knockdown suppressed cell tumorigenesis in NPC via regulating miR-338-3p/HRAS axis, suggesting a novel therapeutic strategy for NPC.

20.
J Cell Mol Med ; 2020 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-32961025

RESUMO

Diabetes is a disorder of glucose metabolism, and over 90% are type 2 diabetes. Diabetic cardiomyopathy (DCM) is one of the type 2 diabetes complications, usually accompanied by changes in myocardial structure and function, together with cardiomyocyte apoptosis. Our study investigated the effect of curcumin on regulating oxidative stress (OS) and apoptosis in DCM. In vivo, diabetes was induced in an experimental rat model by streptozoticin (STZ) together with high-glucose and high-fat (HG/HF) diet feeding. In vitro, H9c2 cardiomyocytes were cultured with high-glucose and saturated free fatty acid palmitate. Curcumin was orally or directly administered to rats or cells, respectively. Streptozoticin -induced diabetic rats showed metabolism abnormalities and elevated markers of OS (superoxide dismutase [SOD], malondialdehyde [MDA], gp91phox , Cyt-Cyto C), enhanced cell apoptosis (Bax/Bcl-2, Cleaved caspase-3, TUNEL-positive cells), together with reduced Akt phosphorylation and increased Foxo1 acetylation. Curcumin attenuated the myocardial dysfunction, OS and apoptosis in the heart of diabetic rats. Curcumin treatment also enhanced phosphorylation of Akt and inhibited acetylation of Foxo1. These results strongly suggest that apoptosis was increased in the heart of diabetic rats, and curcumin played a role in diabetic cardiomyopathy treatment by modulating the Sirt1-Foxo1 and PI3K-Akt pathways.

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