Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 3.425
Filtrar
1.
J Cell Biochem ; 121(1): 713-722, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31385350

RESUMO

Small for gestational age (SGA) has a high risk of mortality and morbidity and is common in obstetrics. To date, no effective prediction and treatment tools are available. Acting as microRNA (miRNA) sponges and disease biomarkers are clear functions of circular RNAs (circRNAs). However, it is still unknown what role circRNAs act in SGA. To explore the role of circRNAs in SGA, circRNA expression patterns of the umbilical cord and maternal plasma in SGA was assessed. We first evaluated circRNAs in umbilical cord blood of the SGA and appropriate for gestational age (AGA) groups by microarray sequencing. In total, 170 340 circRNAs were sequenced, and 144 circRNAs were significantly upregulated while 977 were markedly downregulated. Has_circRNA15994-13, has_circ_0001359, and has_circ_0001360 were abundant and differentially expressed between the SGA and AGA groups, and confirmed in the umbilical cord and maternal blood specimens by reverse transcription polymerase chain reaction. By combining miRNA microarray data of the SGA placenta tissue in NCBI, it was found that two miRNAs were both hsa_circRNA15994-13 targets and differentially expressed, including hsa-miR-3619-5p and hsa-miR-4741. Further KEEG analysis revealed that the most significant pathway enriched by hsa-miR-3619-5p was Wnt signaling that is closely related to SGA; meanwhile, previous reports demonstrated that hsa-miR-3619-5p directly binds to ß-catenin to accommodate the Wnt/ß-catenin pathway, whereby the suggestive hsa_circRNA15994-13 → hsa-miR-3619-5p → ß-catenin signaling pathway may play an important part in SGA.

2.
J Hazard Mater ; 382: 121057, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31470300

RESUMO

Zeolitic imidazolate frameworks (ZIFs) possess tremendous potential in various adsorption and catalysis areas for their particular structures. However, the dispersibility and acid stability of ZIFs are important issues hindering their applications. To address these challenges, a transparent polydimethysiloxane (PDMS) coating was constructed to heterogeneously anchor the Cu doped ZIF-67 (Cu/ZIF-67) nanoparticles on melamine sponge surface, achieving a PDMS-coated ZIF three-dimensional composite sponge. Thus PDMS coating could also effectively protect ZIFs from acid damage to prolong the service life of photocatalyticity. It was demonstrated that the composite sponges were able to repeatedly (over 40 cycles) degrade Sudan I dyes with remarkable photocatalytic efficiency (>97%). More importantly, the ion impenetrability of PDMS coating made the ZIFs based composite a longer term catalytic life than unprotected Cu/ZIF-67 under acid condition. Incidentally, due to the introduction of rough ZIFs and hydrophobic PDMS coating, the obtained sponge also exhibits super-hydrophobicity (158.5°), great compressibility and excellent oil/acid water separation performance. We expect that such a polymer coating strategy could act as a novel inspiration for extending the applications and life span of ZIF-based composites.

3.
Arch Gerontol Geriatr ; 86: 103965, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31683176

RESUMO

BACKGROUND: Centenarians are the fastest growing population worldwide. However, this group has been less studied in developing countries. Contemporary centenarians in China have experienced many ups and downs due to historical reasons, which may have resulted in a population with different characteristics from those in other countries. This study aimed to investigate the current sociodemographic characteristics, health profiles, and social relationships of Chinese centenarians. METHODS: We conducted face-to-face surveys in April 2017 with centenarian residents in Suixi County, the first "International Healthy Longevity Area" in China. A total of 100 centenarians were involved, including 67 females and 33 males. Information for socioeconomic and demographics characteristics, quality of life (physical, cognitive, and psychological function), and social support and relationships was collected. Sex differences in each measure were examined. RESULTS: We find that good self-reported health, good life satisfaction, intact memory function, independence, and unsatisfied healthcare needs were reported by 24.4%, 45.9%, 31.6%, 46.3%, and 33.4% of the respondents respectively. Subjective symptoms among males were less prevalent (p < 0.05). There were no statistical significant sex differences in cognitive and psychological function. The major source of care provision has been family. Generally, the centenarians had intimate relationships within families but maintained distant relationships with friends and communities. CONCLUSION: Our results bring attention to family-based care to provide informal care, and health education to promote healthy behaviors and healthcare utilization, for the oldest-old in China. The findings also imply a crucial role of good relationships with family in exceptional longevity.

5.
Carbohydr Polym ; 228: 115370, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31635728

RESUMO

Nanofiber-based materials have recently gained increasing attention in food packaging, drug delivery, and biomedical applications. In this study, a multi-nanofibers composite film was developed based on bacterial cellulose nanofiber (BCNF)/chitin nanofiber (CNF) hybridization. The nanofibers were responsible for the formation of well-dispersed curcumin (Cur) micro/nanoparticles in the nanocomposite films. The release of Cur from the films were affected by CNF and the sizes of Cur particles formed in situ. The Cur particles reduced tensile strength and increased water vapor permeability of BCNF film. However, CNF improved the mechanical strength and barrier property of the Cur/BCNF/CNF composite film. Moreover, the multi-nanofibers composite film showed excellent dynamic antioxidant capacity and antibacterial activity, as well as was capable to monitor pH change and trace amount of boric acid. Results of this study suggested that the Cur/BCNF/CNF composite film can be used as a smart and active food packaging material.

6.
Ann Thorac Surg ; 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31794736

RESUMO

For McKeown esophagectomy, gastric tube is widely used for constructing esophagogastrotomy. Traditionally, the gastric fundus is preserved for gastric tube formation in order to provide adequate length of the gastric conduit for neck anastomosis. However, gastric stump necrosis, anastomotic leakage, and postoperative gastric tube dilatation are the common postoperative complications for McKeown esophagectomy using traditional gastric tube. Here, we reported a novel coniform gastric tube shaping technique to maximally avoid conduit dilatation after the McKeown esophagectomy without nasogastric tube placement, while simultaneously maintain a tension-free and well-perfused anastomosis.

7.
Sci Total Environ ; : 135233, 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31787276

RESUMO

Polystyrene (PS) is one of the major plastic debris accumulated in environment. Previously, we reported that mealworm (Tenebrio molitor) was capable of degrading and mineralizing Styrofoam (PS foam). This finding arouses our curiosity to explore whether more other insect species have the same capability as mealworms. Here, an insect larva, superworm (Zophobas atratus), was newly proven to be capable of eating, degrading and mineralizing PS. Superworms could live with Styrofoam as sole diet as well as those fed with a normal diet (bran) over a 28-day period. The average consumption rate of Styrofoam for each superworm was estimated at 0.58 mg/d that was 4 times more than that of mealworm. Analyses of frass, using gel permeation chromatography (GPC), solid-state 13C cross-polarization/magic angle spinning nuclear magnetic resonance (CP/MAS NMR) spectroscopy, and thermogravimetric interfaced with Fourier transform infrared (TG-FTIR) spectroscopy, demonstrated that the depolymerization of long-chain PS molecules and the formation of low molecular-weight products occurred in the larval gut. A respirometry test showed that up to 36.7% of the ingested Styrofoam carbon was converted into CO2 during a 16-day test period. The PS-degrading capability of superworm was inhibited by the antibiotic suppression of gut microbiota, indicating that gut microbiota contributed to PS degradation. This new finding extends the PS-degrading insects beyond the species within the Tenebrio genus and indicates that the gut microbiota of superworm would be a novel bioresource for pursuit of plastic-degrading enzymes.

8.
J Oral Pathol Med ; 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31788854

RESUMO

BACKGROUND: Small extracellular vesicles (SEVs), have a diameter between 30-150nm and play a key role in cell-cell communication. As cells cultured in 3D versus 2D behave differently, this project aimed to assess whether there were differences in SEVs derived from human oral mucosa lamina propria-progenitor cells (OMLP-PCs) cultured in a 3D matrix compared to traditional 2D monolayer cultures. METHODS: OMLP-PCs were cultured in 3D type I collagen matrices or on traditional 2D tissue culture plastic. Cell morphology and viability were assessed by light microscopy, actin staining and trypan blue staining. SEVs secreted by OMLP-PCs were purified and quantitatively analyzed by a BCA assay and nanoparticle tracking analysis (NTA; nanosight™). SEVs were further characterized by flow cytometry. SEV proliferative function was assessed by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. RESULTS: Cells cultured in 3D grew well as observed by light microscopy and phalloidin staining with cells branching in three-dimensions (as opposed to the cells grown as monolayers on tissue culture plastic). NTA demonstrated a significantly higher number of SEV-sized particles in the conditioned medium of cells grown in 3D type I collagen matrices versus a 2D monolayer (P<0.01). Like SEVs from 2D culture, SEVs from 3D culture demonstrated a particle size within the expected SEV range. Tetraspannin analysis confirmed that 3D-derived SEVs were positive for typical, expected tetraspannins. Cell proliferation analysis demonstrated that SEVs produced through 3D cell culture conditions, significantly reduced the proliferation of skin fibroblasts when compared to SEVs from 2D monolayers (P<0.05). CONCLUSION: 3D culture of OMLP-PCs produced typical SEVs but in a greater amount than when the same cells were cultured in 2D. The downstream proliferative potential of the SEVs was influenced by the initial culture methodology. Future work should now assess the potential effects of 3D SEVs on key wound healing activities.

9.
Adv Mater ; : e1907088, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31788889

RESUMO

Future wearable electronics requires safe and high-energy-density supercapacitors (SCs). Commercial SCs making use of organic electrolytes show high energy density, but the flammability of the electrolyte raises serious safety concerns. Aqueous SCs, on the other hand, are very safe, but the energy density is low due to the much narrower voltage window and the difficulty of fabricating thick electrodes. A new materials strategy named soft hybrid scaffold (SHS), which allows easy buildup of ultrathick electrodes made of 3D porous pseudo-material-modified carbon networks, is reported. The carbon network provides excellent mechanical stability and electric conductivity, the hierarchically porous structures ensure rapid ionic transport, and the pseudomaterials enlarge the electrochemical window. Asymmetric aqueous SCs using SHS electrodes show higher energy density than both commercial organic SCs and literature-reported aqueous SCs, with good cycle life and mechanical flexibility. The aqueous SC device is tailorable, waterproof, and fire-retardant, representing a high safety toward practical applications.

10.
Artigo em Inglês | MEDLINE | ID: mdl-31801197

RESUMO

BACKGROUND: Air pollutants cause endocrine disorders and hormone disruption. The relationship between air pollutants and polycystic ovary syndrome (PCOS) must be carefully investigated using a nationwide cohort. METHODS: Data were extracted from two nationwide databases, namely Longitudinal Health Insurance Database and Taiwan Air Quality Monitoring Database, and analyzed. The study considered a range of data that began on 1 January 2000 and ended on 31 December 2013. Women diagnosed with PCOS were excluded. From the residential data, the study assessed the daily concentrations of sulfur dioxide (SO2), nitrogen oxides (NOx), nitrogen monoxide (NO), nitrogen dioxide (NO2), and PM2.5 the women were exposed to. A Cox proportional hazard regression model was applied to assess PCOS risk. RESULTS: In total, 91,803 women were enrolled in this study; of those women, 2072 developed PCOS after 12 years of follow-up. The mean daily concentrations of SO2, NOx, NO, NO2, and PM2.5 women were exposed to were 4.25 (±1.44) ppb, 20.41 (±6.65) ppb, 9.25 (±4.36) ppb, 20.99 (±3.33) ppb, and 30.85 (±6.16) µg/m3, respectively. Compared with the first-quartile levels of exposure, the fourth-quartile levels of exposure to SO2, NOx, NO, NO2, and PM2.5 increased PCOS risk by 10.31 times (95% CI = 8.35-12.7), 3.37 times (95% CI = 2.86-3.96), 4.18 times (95% CI = 3.57-4.89), 7.46 times (95% CI = 6.38-8.71), and 3.56 times (95% CI = 3.05-4.15), respectively. CONCLUSION: Women exposed to a high concentrations of air pollutants, namely SO2, NO, NO2, NOx, and PM2.5, had a high PCOS risk.

11.
Arthritis Res Ther ; 21(1): 267, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31801580

RESUMO

BACKGROUND: The information concerning non-invasive, easily obtainable, and accurate biomarkers for diagnosis of lupus nephritis (LN) is extremely limited. The aim of this study was to evaluate the diagnostic performance of cystatin C (CysC) and complement component 1q (C1q) for LN. METHODS: A case-control study that included 905 patients with systemic lupus erythematosus (SLE) without LN (group SLE), 334 patients with active lupus nephritis (group LNA), 255 patients with inactive lupus nephritis (group LNI), and 497 healthy individuals (group HC) was performed in Mianyang Central Hospital from March 2017 to December 2018. The serum levels of CysC, C1q, urea (Urea), and creatinine (Creat) were measured, and 2 estimated glomerular filtration rates (eGFRCysC and eGFRCreat) were calculated by equations which were based on serum CysC established by our group and the modification of diet in renal disease (MDRD), respectively. ANOVA analysis or Kruskal-Wallis test was used for comparing the differences among the groups, and receiver operating characteristic (ROC) curve was applied to identify the diagnostic efficiencies of individual or combined multiple indicators. RESULTS: Significantly elevated CysC and decreased C1q were observed in the LNA and LNI groups, which was in contrast to their levels in the SLE and HC groups. CysC (AUC = 0.906) or eGFRCysC (AUC = 0.907) assessed the highest diagnostic performance on LNA when detected individually, followed by C1q (AUC = 0.753). Joint utilization of C1q and CysC achieved very good performance (AUC = 0.933) which approximated to the best one observed in the combinations of C1q, Urea, CysC, eGFRCreat, and Creat (AUC = 0.975). CONCLUSION: The separately detected CysC (eGFRCysC) and C1q were superior to the conventional biomarkers Urea, Creat, and eGFRCreat in the diagnosis of LNA. Moreover, although the combined detection of Urea, Creat, C1q, CysC, and eGFRCreat had the greatest diagnostic performance, the joint utilization of CysC and C1q could be prioritized for rapid discrimination of LNA if the economic burden is taken into consideration.

12.
EMBO J ; : e102602, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31802519

RESUMO

Plants establish mutualistic associations with beneficial microbes while deploying the immune system to defend against pathogenic ones. Little is known about the interplay between mutualism and immunity and the mediator molecules enabling such crosstalk. Here, we show that plants respond differentially to a volatile bacterial compound through integral modulation of the immune system and the phosphate-starvation response (PSR) system, resulting in either mutualism or immunity. We found that exposure of Arabidopsis thaliana to a known plant growth-promoting rhizobacterium can unexpectedly have either beneficial or deleterious effects to plants. The beneficial-to-deleterious transition is dependent on availability of phosphate to the plants and is mediated by diacetyl, a bacterial volatile compound. Under phosphate-sufficient conditions, diacetyl partially suppresses plant production of reactive oxygen species (ROS) and enhances symbiont colonization without compromising disease resistance. Under phosphate-deficient conditions, diacetyl enhances phytohormone-mediated immunity and consequently causes plant hyper-sensitivity to phosphate deficiency. Therefore, diacetyl affects the type of relation between plant hosts and certain rhizobacteria in a way that depends on the plant's phosphate-starvation response system and phytohormone-mediated immunity.

14.
Molecules ; 24(21)2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31683952

RESUMO

Four new constituents, as cis-6-oxogeran-4-enyl-10-oxy-O-ß-arabinopyranosyl-4'-O-ß-arabinopyranosyl-2''-octadec-9''',12''',15'''-trienoate (1), geran-3(10)-enyl-1-oxy-O-ß-arabinopyranosyl-4'-O-ß-arabinopyranosyl-2''-octadec-9''',12''',15'''-trienoate (2), geranilan-8-oxy-O-α-d-xylopyranosyl-2'-n-octadec-9'',12'',15''-trienoate (3), 1-cyclohex-2', 5'-dienyl 1-cyclohexylethanol-O-ß-d-xylopyranoside (4), along with six known constituents, guaiacol-O-ß-d-arabinopyaranoside (5), n-tetradecanyl oleate (6), oleyl-O-ß-d-xyloside (7), n-octadec-9,12-dienoyl-O-ß-d-arabinopyranoside (8), linolenyl-O-ß-d-arabinofuranoside (9) andglyceryl-1,3-dipalmito-2-olein (10), were isolated and identified from the Dendropanax morbifera bark. The new structures were established by one-and two-dimensional NMR (and in combination with IR, FAB-MSand HR-ESI-FTMS. The comparative evaluation of antioxidant potential by phosphomolybdenum, DPPH, FRAP and the NO assay of four different compounds (1-4), we have found that the compounds 1 and 2 have power as a natural antioxidant, whereas the compound 3 and 4 exhibited mild activity in comparison to compounds 1 and 2.

15.
J Clin Anesth ; : 109631, 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31669050

RESUMO

STUDY OBJECTIVE: Dexmedetomidine, a highly selective α2-receptor agonist, has been widely used for protection against ischemia-reperfusion (IR) injury. We hypothesized that dexmedetomidine might exert a protective effect on IR injury after hepatectomy. DESIGN: A prospective, randomized, single-blind study was conducted in 58 patients undergoing hepatectomy who were randomly assigned to two study groups. The dexmedetomidine group (D group) received a loading dose of 0.5 µg/kg for 10 min, and maintained it with 0.5 µg/kg/h until resection of the liver lobes. The control group (C group), received 0.9% sodium chloride administered in the same volume and infusion rate as D group. Eleven patients had hepatic inflow occlusion in D group as did 14 patients in C group. MEASUREMENTS: The primary outcome was the serum concentration of α-glutathione S-transferase (α-GST), which reflects hepatic ischemic injury. Secondary outcomes included laboratory variables reflecting inflammatory responses, liver and kidney function, and blood coagulation, as well as hemodynamic changes, recovery variables, and complications related to anesthesia and surgery. RESULTS: The concentration of α-GST at 0.5 h after resection was significantly lower in the dexmedetomidine group than the control group (9.1 ±â€¯3.4 ng/mL vs 15.8 ±â€¯6.5 ng/mL; p < .01), and was also significantly lower in the dexmedetomidine group in subgroup analyses of patients with and without hepatic inflow occlusion. While the concentrations of α-GST at 0.5 h after resection in patients with or without occlusion in D group were comparable, in C group the α-GST concentration without occlusion was significantly higher than that with occlusion. There was an interaction between dexmedetomidine and no occlusion (p < .01), and its concentration in D group without occlusion was the lowest of all subgroups. In addition, there were significant differences in interleukin (IL)-6 and tumor necrosis (TNF)-α concentrations at 24 h after hepatectomy between the two groups, and mean arterial pressure, heart rate, and the bispectral index were also significantly lower in D group than in C group (p < .05). There were significant differences between the two groups in ALT and AST at 2 h and 24 h after the resection of the liver lobe. However, there were no significant differences in renal function, recovery variables, blood coagulation. No severe complications related surgeries and anesthesia were found in both groups. CONCLUSION: Dexmedetomidine exerts a protective effect on ischemia-reperfusion injury after hepatectomy.

16.
Chaos ; 29(11): 113126, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31779352

RESUMO

Driver fatigue is an important cause of traffic accidents, which has triggered great concern for detecting drivers' fatigue. Numerous methods have been proposed to fulfill this challenging task, including feature methods and machine learning methods. Recently, with the development of deep learning techniques, many studies achieved better results than traditional feature methods, and the combination of traditional methods and deep learning techniques gradually received attention. In this paper, we propose a recurrence network-based convolutional neural network (RN-CNN) method to detect fatigue driving. To be specific, we first conduct a simulated driving experiment to collect electroencephalogram (EEG) signals of subjects under alert state and fatigue state. Then, we construct the multiplex recurrence network (RN) from EEG signals to fuse information from the original time series. Finally, CNN is employed to extract and learn the features of a multiplex RN for realizing a classification task. The results indicate that the proposed RN-CNN method can achieve an average accuracy of 92.95%. To verify the effectiveness of our method, some existing competitive methods are compared with ours. The results show that our method outperforms the existing methods, which demonstrate the effect of the RN-CNN method.

17.
Protoplasma ; 2019 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-31760482

RESUMO

As a perennial herbaceous species, Panax ginseng is widely cultivated and used as traditional herbal medicine. The root of Panax ginseng commonly remains expensive as conventional breeding of Panax ginseng is difficult. Somatic embryogenesis (S.E.) is a useful tool for plant propagation and optimal model for understanding the mechanisms of plant embryogenesis. In Panax ginseng, increasing studies have been widely performed to optimize the technology of S.E., while the underlying mechanism remains unclear. In this paper, we cloned and identified a WRKY family gene named PgWRKY6 which is upregulated in response to 2,4-D (2,4-dichlorophenoxyacetic acid)-induced embryogenic callus development. The silencing of PgWRKY6 obviously reduces the induction rate of embryogenic callus, indicating its crucial role in S.E. of Panax ginseng hairy root. The expressions of several ROS-scavenging genes are also inducible during embryogenic callus development, and the transcriptions of PgGST, PgAPX1, and PgSOD are demonstrated to be regulated by PgWRKY6. Recombinant PgWRKY6, an approximate 40-KDa protein purified from Escherichia coli, shows a specific DNA-binding activity with a potential recognition site of TTGAC(C/T). This work demonstrated that as a conserved WRKY family transcription factor, PgWRKY6 functions upstream of PgGST, PgAPX1, and PgSOD, and potentially mediated auxins -ROS signaling pathway in the process of S.E. in Panax species.

18.
J Diabetes Complications ; : 107468, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31761418

RESUMO

AIMS: To investigate the effect of multifactorial intervention on the urinary albumin to creatinine ratio (UACR) and the estimated glomerular filtration rate (eGFR) in short-duration type 2 diabetes. METHODS: A total of 150 type 2 diabetes patients, with disease duration <1 year and with no evidence of atherosclerosis were randomized to either the intensive intervention group (IG, n = 75), or the conventional group (CG, n = 75) for 7 years. The predefined endpoint of microvascular complications was the progression of renal impairments (the development of albuminuria and the change of eGFR). RESULTS: The incidence of progression to albuminuria (UACR ≥30 mg/g) was 12% in IG and 28% in CG (HR 0.37, 95% CI: 0.19-0.70, P = .0025). eGFR was significantly lower in IG than that in CG in the year 2 (P = .043) and 3 (P = .032) follow-up. Sex, fasting plasma glucose (FPG), HbA1c, and systolic blood pressure (SBP) were independently associated with the UACR (ß = -5.112, P = .015; ß = 0.908, P = .045; ß = 2.087, P = .038; and ß = 2.787, P = .002, respectively); aging was independently associated with eGFR (ß = -0.447, P = .000). CONCLUSIONS: Intensive multifactorial intervention delayed the progression to albuminuria, and reduced eGFR rapidly in early stage of intervention in short-duration type 2 diabetes. FPG, HbA1c, and SBP were risk factors for UACR increase; aging was a risk factor for eGFR decline.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31699591

RESUMO

In this work, a primary method was constructed for detecting hydrazine in plant, thus accomplished the closed-loop monitoring of hydrazine circulation within manufacture, environment, plants, animals and human. From a series of sensors, QYL-1 was selected to present the hydrazine sensing properties. As a preliminary tool, QYL-1 suggested the ultra-wide linear range (0-20.0 equivalent) and high selectivity, which were extremely essential for linking the monitoring in various scale and field. For the first time, concentration-dependent tracking of hydrazine was successfully performed in Arabidopsis Thaliana root tips. Afterwards applications in water samples and living MCF-7 cells then fulfilled the demonstration of closing the loop by linking both the upstream and downstream nodes. More than raising a practical method, this work offered initial information for the closed-loop monitoring of hydrazine circulation, which might be significant for the ideal systematic managing in future.

20.
Artigo em Inglês | MEDLINE | ID: mdl-31701376

RESUMO

Agro-industrial wastes are excellent sources for solid-state culture to produce spores of microorganisms, whereas microbial co-cultivation is not fully exploited in solid-state culture. In this work, the co-cultivation of different strains of Bacillus subtilis, and three microbes of B. subtilis, Bacillus mucilaginosus, and Paecilomyces lilacinus was studied using a solid medium only composed of water and tobacco waste residue after extraction of nicotine and solanesol. The influences of matrix thickness, moister, temperature, and ratio of three microbes in seed on the cell growth and spore formation were studied. The maximum viable cells and spores of each microbe reached 1013 cfu/g when cultured alone at 30 °C in a medium containing 58.3% moisture. Co-cultivation of microbes stimulated cell growth and maximum viable cells of each microbe reached 1014 cfu/g, while spore production was inhibited and decreased to 1011 cfu/g. With decreasing amount of P. lilacinus in seed, total amount of spores was increased. When the seed with a ratio of 6:3:1 for B. mucilaginosus, B. subtilis, and P. lilacinus was inoculated, the total amount of spores reached 4.14 × 1012 cfu/g and the ratio was 1.7:0.7:1. These results indicate the potential of solid-state cultivation in the high production of spores from tobacco waste residue at low cost.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA