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1.
J Nanosci Nanotechnol ; 20(3): 1478-1485, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31492310

RESUMO

The loop-mediated isothermal amplification (LAMP) assay as efficient and convenient detection method was applied to detect the vacuolating cytotoxin A (vacA) gene of Helicobacter pylori (H. pylori). The extracted genomic DNA of H. pylori, which was purified through magnetic nanoparticles (MNPs), was amplified through the LAMP reaction using designed primers. The effect of LAMP detected on H. pylori vacA gene was evaluated through agarose gel electrophoresis in a gel imaging system and fluorescence-intensity analysis after addition of fluorescent dye. 11 pathogenic bacterial strains of different species were found to be negative for vacA, while only a single positive result was obtained for H. pylori. The minimum detection limit of the vacA gene was established as 100 fg. We used the primers with specificity and sensitivity, which were designed by the specificity analysis and sensitivity analysis system. Once developed, the LAMP assay was be used to the detection of the vacA gene in the gastric juice of patients. In conclusion, the LAMP assay is an efficient and fast tool for detection of the H. pylori vacA gene, and also for direct detection of the vacA gene in the gastric juice of patients, with high sensitivity and specificity. Most importantly, the proposed detection method shows promising potential for clinical application in the future, where it can greatly reduce the difficulty of detection and also shorten detection times.

2.
Environ Technol ; : 1-23, 2019 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-31580199

RESUMO

This study aimed to investigate the effects of red mud based-passivator (RM based-passivator) on rice yield, cadmium (Cd) in brown rice, pH and available Cd in Cd-polluted soil by pot experiments, and to explore the adsorption mechanism of the passivator by scanning electron microscope (SEM), energy dispersive X-ray spectroscopy (EDX), fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) analysis. The results showed that this passivator obviously improved the pH, reduced the available Cd in soil and the Cd content in brown rice in Changsha Cd polluted soil (CS soil), while had little effect on Hengyang Cd polluted soil (HY soil). Compared with the control, the soil pH increased in the range from 0.31 to 1.37, the available Cd in soil decreased in the range from 13.25% to 52.34%, and the total Cd in brown rice decreased in the range from 46.44% to 84.98 % in CS soil. Considering the impacts of RM based-passivator on the growth of rice, the Cd content in brown rice, and the pH and available Cd in soil, 0.10-0.30% (w/w) of the passivator is recommended to apply in CS soil but not in HY soil. Based on the analysis of SEM, EDX, FTIR, XRD, the mechanism of Cd(II) adsorption by RM based-passivator included physical adsorption, surface complexation, and ion exchange. The present results indicated that the appropriate addition of RM based-passivator could be an effective strategy for the remediation of acidic Cd-polluted soils.

3.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 36(10): 1031-1034, 2019 Oct 10.
Artigo em Chinês | MEDLINE | ID: mdl-31598954

RESUMO

OBJECTIVE: To emphasize the clinical significance of copy number variations (CNVs) detection by describing a case misdiagnosed as trisomy 21 syndrome by G-banded chromosomal karyotype analysis. METHODS: A girl with obesity and short stature was diagnosed as trisomy 21 syndrome by G-banded chromosomal karyotype analysis. Considering the discrepancy of her karyotype with her phenotype, genomic CNVs was detected by next-generation sequencing and the result was verified by quantitative PCR (qPCR). RESULTS: A microduplication of 16p11.2: 29 642 339-29 775 631 (133.292 kb) was detected. qPCR assay for QPRT and SPN located in the duplicated region confirmed the finding of CNVs assay. Meanwhile, her parents did not present similar duplication in 16p11.2. CONCLUSION: The 16p11.2 microduplication was a novel genomic structural variation in the girl, though it may not be associated with her clinical manifestations. Chromosomal microarray or next-generation sequencing-based CNVs detection can accurately determine the origin of small supernumerary marker chromosome and reduce the chance of misdiagnosis.

4.
Adv Mater ; : e1902807, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31566828

RESUMO

1D nanomaterials with high aspect ratio, i.e., nanowires and nanotubes, have inspired considerable research interest thanks to the fact that exotic physical and chemical properties emerge as their diameters approach or fall into certain length scales, such as the wavelength of light, the mean free path of phonons, the exciton Bohr radius, the critical size of magnetic domains, and the exciton diffusion length. On the basis of their components, aspect ratio, and properties, there may be imperceptible connections among hundreds of nanowires prepared by different strategies. Inspired by the heredity system in life, a new concept termed the "nanowire genome" is introduced here to clarify the relationships between hundreds of nanowires reported previously. As such, this approach will not only improve the tools incorporating the prior nanowires but also help to precisely synthesize new nanowires and even assist in the prediction on the properties of nanowires. Although the road from start-ups to maturity is long and fraught with challenges, the genetical syntheses of more than 200 kinds of nanostructures stemming from three mother nanowires (Te, Ag, and Cu) are summarized here to demonstrate the nanowire genome as a versatile toolbox. A summary and outlook on future challenges in this field are also presented.

5.
Rev Sci Instrum ; 90(9): 095001, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31575224

RESUMO

The uncertainty, complexity, and variability of the marine environment inevitably lead to a change in the measurement error resulting in erroneous estimation of navigation information. To solve this problem, this paper proposes a novel method integrating the square-root cubature Kalman filter (SCKF) with the expectation-maximization (EM) algorithm. The proposed new SCKF (NSCKF) algorithm makes better use of the advantages of SCKF and the EM online algorithm. The performance of NSCKF is verified theoretically and evaluated by experiments. The results indicate that the proposed NSCKF algorithm can better estimate predicted error covariance and measurement noise than two other comparison methods owing to the online EM method so that the more accurate attitude estimation can be obtained by the NSCKF algorithm although the measurement error has a great variation. Moreover, the accuracy and efficiency can be guaranteed by employing the SCKF. Experimental results demonstrate that the NSCKF can provide a more stable attitude estimation in different cases of measurement errors. Therefore, the NSCKF is more suitable to be used in underwater navigation than other comparison methods because of higher accuracy, more efficiency, and better robustness.

6.
Psychiatr Serv ; : appips201900106, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31575350

RESUMO

OBJECTIVE: The aim of this systematic review and meta-analysis was to examine the prevalence of voluntary and involuntary psychiatric admissions for severe mental illness in China and explore their associated factors. METHODS: The PubMed, Cochrane Library, PsycINFO, EMBASE, CNKI, CQVIP, and WanFang databases were systematically searched for the span since their inception to October 2018. Meta-analyses were conducted with the random-effects model. RESULTS: Fourteen studies with 94,305 patients were included in the analyses. The voluntary and involuntary admission rates were 30.3% (95% confidence interval [CI]=18.06% to 46.23%) and 32.3% (95% CI=10.39% to 66.21%), respectively, for severe mental illness and 19.6% (95% CI=14.09% to 26.54%) and 44.3% (95% CI=5.98% to 90.88%), respectively, for schizophrenia. Meta-regression analyses found an increase in the voluntary admission rate between 1998 and 2018. CONCLUSIONS: The voluntary admission rate for severe mental illness has increased in China, whereas the involuntary admission rate has remained high, particularly for schizophrenia. Concerted efforts should be made to further decrease the rate of involuntary admissions.

7.
Medicine (Baltimore) ; 98(40): e17406, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31577751

RESUMO

Serum ferritin (SF) has been identified as a potential prognostic factor for patients undergoing stem cell transplantation, but the prognostic value of SF in acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS) patients and the impact of iron chelation therapy (ICT) on MDS patients are controversial. The present meta-analysis aimed to better elucidate these relationships.Three electronic databases were searched systematically to identify reports on the prognostic role of SF in MDS and AML patients, and those investigating the impact of ICT on prognosis of MDS patients. The hazard ratios (HRs) and its 95% confidence interval (95%CI) were extracted from the identified studies using Cox proportional hazard regression model for overall survival (OS) and progression of MDS to AML.Twenty reports including 1066 AML patients and 4054 MDS patients were included in present study. The overall pooled HRs for OS of AML and MDS patients with elevated SF prior to transplantation was 1.73 (1.40-2.14), subgroup analyses stratified by the cut-off value of SF ≥1400/1000 ng/mL showed that the pooled HRs were 1.45 (0.98-2.15) and 1.65 (1.30-2.10), respectively. The pooled HRs for ICT in MDS patients was 0.30 (0.23-0.40). For ICT, the pooled HRs for the progression of MDS to AML was 0.84 (0.61-1.61).SF has a negative impact on the OS of AML and MDS patients when it is higher than 1000 ng/mL. ICT can improve the OS of MDS patients with iron overload but it is not associated with the progression of MDS to AML.

8.
Clin Cancer Res ; 2019 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-31582516

RESUMO

PURPOSE: Transforming growth factor-ßs (TGF-ßs) are overexpressed in many advanced cancers and promote cancer progression through mechanisms that include suppression of immunosurveillance. Multiple strategies to antagonize the TGF-ß pathway are in early phase oncology trials. However, TGF-ßs also have tumor suppressive activities early in tumorigenesis, and the extent to which these might be retained in advanced disease has not been fully explored. EXPERIMENTAL DESIGN: A panel of twelve immunocompetent mouse allograft models of metastatic breast cancer was tested for the effect of neutralizing anti-TGF-ß antibodies on lung metastatic burden. Extensive correlative biology analyses were performed to assess potential predictive biomarkers and probe underlying mechanisms. RESULTS: Heterogeneous responses to anti-TGF-ß treatment were observed, with 5/12 models (42%) showing suppression of metastasis, 4/12 (33%) showing no response and 3/12 (25%) showing an undesirable stimulation (up to 9-fold) of metastasis. Inhibition of metastasis was immune-dependent, while stimulation of metastasis was immune-independent and targeted the tumor cell compartment, potentially affecting the cancer stem cell. Thus the integrated outcome of TGF-ß antagonism depends on a complex balance between enhancing effective anti-tumor immunity and disrupting persistent tumor suppressive effects of TGF-ß on the tumor cell. Applying transcriptomic signatures derived from treatment-naive mouse primary tumors to human breast cancer datasets suggested that breast cancer patients with high-grade, estrogen receptor-negative disease are most likely to benefit from anti-TGF-ß therapy. CONCLUSIONS: Contrary to dogma, tumor suppressive responses to TGF-ß are retained in some advanced metastatic tumors. Safe deployment of TGF-ß antagonists in the clinic will require good predictive biomarkers.

9.
J Natl Compr Canc Netw ; 17(10): 1174-1183, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31590148

RESUMO

BACKGROUND: Differences between the features of primary cancer and matched metastatic cancer have recently drawn attention in research. This study investigated the concordance in microsatellite instability (MSI) and mismatch repair (MMR) status between primary and corresponding metastatic colorectal cancer (CRC). METHODS: Consecutive patients with metastatic CRC who had both primary and metastatic tumors diagnosed at our institution in January 2008 through December 2016 were identified. Immunohistochemistry was used to test the MMR status of both primary and matched metastatic tumors, and PCR analysis was performed to test MSI in patients with deficient MMR (dMMR) status. RESULTS: A total of 369 patients were included. Of the 46 patients with MSI-high primary tumors, 37 (80.4%) also had MSI-high metastatic tumors, whereas 9 (19.6%) had microsatellite stable (MSS) metastatic tumors. A high concordance was found in patients with liver, lung, or distant lymph node metastases. Interestingly, the discrepancy was more likely to be limited to peritoneal (5/20) or ovarian (4/4) metastasis (chi-square test, P<.001). These organ-specific features were also found in the pooled analysis. Along with the change of MSI-high in primary cancer to MSS in metastatic cancer, lymphocyte infiltration decreased significantly (P=.008). However, the change did not influence survival; the median overall survival of MSI-high and MSS metastatic tumors was 21.3 and 21.6 months, respectively (P=.774). The discrepancy rate was 1.6% for patients with proficient MMR primary tumors. CONCLUSIONS: For patients with dMMR primary tumors, the concordance of MSI and MMR status in primary CRC and corresponding metastatic cancer is potentially organ-specific. High concordance is found in liver, lung, and distant lymph node metastases, whereas discrepancy is more likely to occur in peritoneal or ovarian metastasis. Rebiopsy to evaluate MSI-high/dMMR status might be needed during the course of anti-PD-1 therapy in cases of peritoneal or ovarian metastasis.

10.
Sensors (Basel) ; 19(19)2019 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-31590250

RESUMO

Image analysis techniques have been applied to measure the displacements, strain field, and crack distribution of structures in the laboratory environment, and present strong potential for use in structural health monitoring applications. Compared with accelerometers, image analysis is good at monitoring area-based responses, such as crack patterns at critical regions of reinforced concrete (RC) structures. While the quantitative relationship between cracks and structural damage depends on many factors, cracks need to be detected and quantified in an automatic manner for further investigation into structural health monitoring. This work proposes a damage-indexing method by integrating an image-based crack measurement method and a crack quantification method. The image-based crack measurement method identifies cracks locations, opening widths, and orientations. Fractal dimension analysis gives the flexural cracks and shear cracks an overall damage index ranging between 0 and 1. According to the orientations of the cracks analyzed by image analysis, the cracks can be classified as either shear or flexural, and the overall damage index can be separated into shear and flexural damage indices. These damage indices not only quantify the damage of an RC structure, but also the contents of shear and flexural failures. While the engineering significance of the damage indices is structure dependent, when the damage indexing method is used for structural health monitoring, the damage indices safety thresholds can further be defined based on the structure type under consideration. Finally, this paper demonstrates this method by using the results of two experiments on RC tubular containment vessel structures.

11.
J Diabetes ; 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31602779

RESUMO

BACKGROUND: We aim to design a scoring model for differential diagnosis between diabetic nephropathy (DN) and non-diabetic renal disease (NDRD) in type 2 diabetic patients through a combination of clinical variables. METHODS: 170 patients with type 2 diabetes who underwent kidney biopsies were included and divided into three groups according to pathological findings: DN group (n=46), MIX group (DN+NDRD, n=54), NDRD group (n=70). Clinical characteristics and laboratory data were collected and compared among groups. Variables with significant statistical difference between DN and NDRD patients were analyzed by logistic regression to predict the presence of NDRD, then a scoring model was established based on regression coefficient and further validated in an independent cohort of 67 patients prospectively. RESULTS: On biopsy, 72.9% of patients had NDRD and the most common pathological type was membranous nephropathy. The established scoring model for predicting NDRD included five predictors: age, systolic blood pressure, hemoglobin, duration of diabetes, absence of diabetic retinopathy. The model demonstrated good discrimination and calibration (area under curve [AUC] 0.863, 95%CI 0.800-0.925; H-L P=0.062). Furthermore, high prediction accuracy (AUC=0.900; 95% CI, 0.815-0.985) in the validation cohort proved the stability of the model. CONCLUSIONS: We present a simple, robust scoring model for predicting the presence of NDRD with high accuracy (0.85) for the first time. This decision support tool provides a noninvasive method for differential diagnosis of DN and NDRD, which may help clinicians assess the risk-benefit ratio of kidney biopsy for type 2 diabetic patients with renal impairment.

12.
Zhongguo Zhong Yao Za Zhi ; 44(15): 3281-3286, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602884

RESUMO

Decoction pieces are important raw materials in the production of traditional Chinese medicine( TCM),and their quality could directly affect the clinical efficacy and medication safety. Research on the production and processing technology of TCM is the basis for the normalization and standardization of Chinese medicine decoction pieces. At present,the production and processing standards for Scutellaria baicalensis pieces are non-regulated,lacking data foundation. In this study,with baicalin,baicalein,wogonoside and wogonin contents as evaluation indicators,single factor experiment was designed to optimize the softening,drying and cutting processes of S. baicalensis,providing a basis for the standardization of their production and processing. The effects of different softening,drying and cutting processes on the contents of the main components in S. baicalensis were comprehensively analyzed by the summation of relative differences. RESULTS:: showed that the contents of the four components and comprehensive indexes were affected by different softening methods and drying temperatures. The content of wogonin in boiling method was higher than that in boiling with cold water,and the content of glycosides in 70 ℃ drying condition was higher than that in other groups. The content of baicalin was significantly affected by different cutting thicknesses,but not by comprehensive index. Eventually,the optimal preparation process for S. baicalensis was determined as follows: boiled in boiling water for 20 min,cut into thin slices( 1-2 mm),and then dried at 70 ℃ in blast drier. This process was close to the actual production,practical and feasible and meanwhile,it was of great significance to improve the quality of S. baicalensis pieces.

13.
J Environ Manage ; 251: 109542, 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31569024

RESUMO

Microbial community is sensitive to the variations of environment, and it plays an important role in biogeochemical cycling in acid mine drainage (AMD). In this study, an integrated high-throughput absolute abundance quantification (iHAAQ) method was applied to study the dynamics of microbial community and the characteristics of microorganism. The results showed a significant difference in bacterial community with diversity being higher in watershed area. The main influential factors for bacterial communities in watershed were physicochemical properties (e.g., pH and potassium), while in mining areas the main driving factors were metals/metalloids (e.g., As, Zn, and Pb). Notably, the major functions of microbial community were transporter and ABC transporter in mining area, while two-component system was more abundant in watershed by the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analysis (level 3). In particular, Phyllobacterium, Bacteroides, and Sulfurovum were demonstrated to be potentially useful bacterial species for bioremediation, which should be a good choice for future studies. These results could facilitate our understanding of microbial diversity in different sediments of mining areas and identify microbial communities for bioremediation projects.

14.
Water Res ; 167: 115137, 2019 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-31585386

RESUMO

Two submerged anaerobic membrane bioreactors (AnMBRs) with and without powdered activated carbon (PAC) were studied to revisit the effect of PAC on membrane fouling performance by long-term operation when treating synthetic sewage. The results showed that PAC remained efficient for membrane fouling control after long-term operation (over 140 d), and it reduced the fouling rate at a hydraulic retention time of 8 h from 3.12 to 0.89 kPa/d. PAC mainly mitigated the membrane fouling by restraining the formation of a cake layer while generating a gel layer on the membrane surface, which was attributed to the PAC-induced microbial community change in mixed liquor and the membrane surface. Microbial community analysis indicated the genera Pseudomonas (26.5%) and Methanothrix (79.21%) were the predominant bacteria and archaea, respectively, in the gel layer, and this result is completely different from the presence of a high abundance of Levilinea (7.1%), Aminivibrio (4.9%) and Methanothrix (90.04%) in the cake layer on the membrane surface without PAC. The significant difference in the predominant microbes in the membrane surface layer was attributed to the reduced enrichment of Levilinea and Methanothrix with PAC addition.

15.
J Cell Mol Med ; 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31490024

RESUMO

MicroRNA (miRNA) critically controls gene expression in many biological processes, including lung growth and pulmonary surfactant biosynthesis. The present study was conducted to investigate whether miR-20a-5p had such regulatory functions on alveolar type II (AT-II) cells. To accomplish this, miR-20a-5p-overexpressed and miR-20a-5p-inhibited adenoviral vectors were constructed and transfected into cultured AT-II cells that were isolated from rat foetal lungs of 19 days' gestation. Transfection efficiency was confirmed by observing the fluorescence of green fluorescent protein (GFP) carried by the viral vector, whereas miR-20a-5p levels were verified by real-time PCR. The CCK-8 assay was used to compare the proliferation ability of AT-II cells that had over- or underexpressed miR-20a-5p. The expression of surfactant-associated proteins (SPs) and phosphatase and tensin homolog (PTEN) was measured by real-time PCR and Western blotting. In AT-II cells, transfection resulted in over- or under-regulation of miR-20a-5p. While overexpression of miR-20a-5p promoted pulmonary surfactant gene expression, its underexpression inhibited it. Consistent with its role in negatively regulating the pulmonary surfactant gene, an opposite pattern was observed for miR-20a-5p regulation of PTEN. As a result, when miR-20a-5p was rendered overexpressed, PTEN was down-regulated. By contrast, when miR-20a-5p was underexpressed, PTEN was up-regulated. Neither overexpression nor underexpression of miR-20a-5p altered the cell proliferation. miR-20a-5p plays no role in proliferation of foetal AT-II cells but is a critical regulator of surfactant gene expression. The latter appears to be achieved through a regulatory process that implicates expression of PTEN.

16.
Artigo em Inglês | MEDLINE | ID: mdl-31482724

RESUMO

Recently, more and more kinds of circulating forms (CRFs) and unique recombinant forms (URFs) identified in the population in China. A novel HIV-1 recombinant virus (2016GXNNIDU016) was identified in this study, which was isolated from an HIV-1 infected intravenous drug user in Nanning, the capital of Guangxi, China. Phylogenetic analysis of the near full-length genome (NFLG) indicated that the sequence (2016GXNNIDU016) was a monophyletic branch that did not cluster with any previously identified genotype. Recombinant analysis showed that the NFLG of 2016GXNNIDU016 is composed of CRF07_BC, CRF01_AE and CRF08_BC, including nine mosaic segments. Differing from previously documented CRF01_AE and CRF07/08_BC recombinant forms, 2016GXNNIDU016 increases the genetic complexity of HIV-1 in Guangxi. The constant emergence of novel recombinant forms should draw our attention to make more efforts in supervising and preventing the spread of HIV infection.

17.
Org Lett ; 21(18): 7284-7288, 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31502462

RESUMO

A novel palladium-catalyzed cascade cyclization of alkene-tethered aryl halides with o-bromobenzoic acids is described, which provides an efficient avenue for building various fused hexacyclic scaffolds containing indolo[2,1-a]isoquinoline in moderate to excellent yield. The method enables the construction of three C-C bonds through an intramolecular carbopalladation, C-H activation, and a decarboxylation sequence. Furthermore, dihydrocyclohepta[de]naphthalene-fused indolo[2,1-a]isoquinolines can be synthesized in moderate yield by constructing a seven-membered ring.

18.
Sci Rep ; 9(1): 13007, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31506476

RESUMO

Diploid strawberry (Fragaria vesca 'Baiguo') is a model plant for studying functional genomics in Rosaceae. Adventitious shoot regeneration is essential for functional genomics by Agrobacterium tumefaciens-mediated transformation. An efficient shoot regeneration method using diploid strawberry leaf explants was conducted on 1/2MS + 1/2B5 medium that contained 2.0 mg L-1 TDZ over 14 days of dark culture; this induced the maximum percentage of shoot regeneration (96.44 ± 1.60%) and the highest number of shoots per explant (23.46 ± 2.14) after 11 weeks of culture. The explants considerably enlarged after 12 days; then, turned greenish brown after 30 days, yellowish brown after 36 days, and completely brown and necrotic after 48 days. Large numbers of adventitious shoots were produced from 48 to 66 days, and the shoots elongated from 66 to 78 days; this represents a critical period of reinvigoration, which included 30 days for leaf explant chlorosis, 36 days for adventitious shoot appearance, and 48 days for generation of numerous shoots. During the reinvigoration process, higher expressions of the hormone synthesis-related genes Ciszog1, CKX2, CKX3, CKX7, YUC2, YUC6, YUC10, YUC9, and GA2ox were detected from 30 to 48 days. Our results indicate that these genes may regulate reinvigoration of shoot regeneration.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31553114

RESUMO

Direct conversion of methane with carbon dioxide to value-added chemicals is attractive but extremely challenging because of the thermodynamic stability and kinetic inertness of both molecules. Herein, the first dinuclear cluster species, RhVO 3 - , has been designed to mediate the co-conversion of CH 4 and CO 2 to oxygenated products, CH 3 OH and CH 2 O, in the temperature range of 393-600 K. The resulting cluster ions RhVO 3 CO - after CH 3 OH formation can further desorb the [CO] unit to regenerate the RhVO 3 - cluster, leading to the successful establishment of a catalytic cycle for methanol production from CH 4 and CO 2 (CH 4 + CO 2 = CH 3 OH + CO). The exceptional activity of Rh-V dinuclear oxide cluster (RhVO 3 - ) identified herein provides a promising way for co-conversion of two very stable molecules CH 4 and CO 2 .

20.
Curr Mol Med ; 2019 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-31530264

RESUMO

Stearic acid (SA), a saturated long-chain fatty acid consisting of 18 carbon atoms, is widely found in feed ingredients such as corn, soybeans, and wheat. However, the roles of SA in the renewal of intestinal epithelial cells remain unclear. In the present study, we found that 0.01-0.15 mM SA promoted IPEC-J2 cell differentiation. In addition, the results showed that the viability of IPEC-J2 cells was inhibited by SA in a time- and dose-dependent manner. Flow cytometry and western blot analysis suggested that SA induced apoptosis and blocked autophagic flux in cells. In addition, the amounts of triglyceride were significantly increased upon challenge with SA. Moreover, the decrease in the viability of cells induced by SA could be attenuated by 4-PBA, an inhibitor of ER stress. In summary, SA accelerated IPEC-J2 cell differentiation at 0.01-0.15 mM. Furthermore, SA induced IPEC-J2 cell apoptosis and impaired autophagic flux by causing ER stress.

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