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1.
J Adv Res ; 2024 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-38527587

RESUMO

INTRODUCTION: With age and ATP decrease in the body, the transcription factors hypophosphorylation weakens the transcription of Slc40a1 and hinders the expression of the iron discharger ferroportin. This may lead to iron accumulation in the brain and the catalysis of free radicals that damage cerebral neurons and eventually lead to Alzheimer's disease (AD). OBJECTIVES: To prevent AD caused by brain iron excretion disorders and reveal the mechanism of J bs-5YP peptide restoring ferroportin. METHODS: We prepared J bs-YP peptide and administered it to the senile mice with dementia. Then, the intelligence of the mice was tested using a Morris Water Maze. The ATP content in the body was detected using the ATP hydrophysis and Phosphate precipitation method. The activation of Slc40a1 transcription was assayed with ATAC seq and the ferroportin, as well as the phosphorylation levels of Ets1 in brain were detected by Western Blot. RESULTS: The phosphorylation level of Ets1in brain was enhanced, and subsequently, the transcription of Slc40a1 was activated and ferroportin was increased in the brain, the levels of iron and free radicals were reduced, with the neurons protection, and the dementia was ultimately alleviated in the senile mice. CONCLUSION: J bs-5YP can recover the expression of ferroportin to excrete excessive iron in the brain of senile mice with dementia by enhancing the transcription of Slc40a1 via phosphorylating Ets1, revealing the potential of J bs-5YP as a drug to alleviate senile dementia.

2.
J Imaging Inform Med ; 2024 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-38321312

RESUMO

Panoramic radiography imaging plays a crucial role in the diagnostic process of dental diseases. However, current artificial intelligence research datasets for panoramic radiography dental image processing are often limited to single-center and single-task scenarios, making it difficult to generalize their results. To address this, we present a multi-center, multi-task labeled dataset. In this study, our dataset comprises three datasets obtained from different hospitals. The first set has 4940 panoramic radiography images and corresponding labels from the Stemmatological Hospital of the General Hospital of Ningxia Medical University. The second set includes 716 panoramic radiography images and labels from the People's Hospital of Yinchuan City, Ningxia. The third dataset contains 880 panoramic radiography images and labels from a hospital in Shenzhen, Guangdong Province. This comprehensive dataset encompasses three types of dental diseases: impacted teeth, periodontitis, and dental caries. Specifically, it comprises 2555 images related to impacted teeth, 2735 images related to periodontitis, and 1246 images related to dental caries. In order to evaluate the performance of the dataset, we conducted benchmark tests for segmentation and classification tasks on our dataset. The results show that the presented dataset could be effectively used for benchmarking segmentation and classification tasks critical to the diagnosis of dental diseases. To request our multi-center dataset, please visit the address: https://github.com/qinxin99/qinxini .

3.
Artigo em Inglês | MEDLINE | ID: mdl-38323905

RESUMO

Cardiac fibrosis is considered as unbalanced extracellular matrix (ECM) production and degradation, contributing to heart failure. Short-chain acyl-CoA dehydrogenase (SCAD) negatively regulates pathological cardiac hypertrophy. The purpose of this study was to investigate the possible role of SCAD in cardiac fibrosis. In-vivo experiments were performed on spontaneously hypertensive rats (SHR) and SCAD knockout mice. The cardiac tissues of hypertensive patients with cardiac fibrosis were used for measurement of SCAD expression. In-vitro experiments, with angiotensin II (Ang II), SCAD siRNA and adenovirus-SCAD (Ad-SCAD) were performed using cardiac fibroblasts (CFs). SCAD expression was significantly decreased in the left ventricles of SHR. Notably, swim training ameliorated cardiac fibrosis in SHR in association with the elevation of SCAD. The decrease in SCAD protein and mRNA expression levels in SHR CFs were in accordance with those in the left ventricular myocardium of SHR. In addition, SCAD expression was downregulated in CFs treated with Ang II in vitro, and SCAD siRNA interference induced the same changes in cardiac fibrosis as Ang II-treated CFs, while Ad-SCAD treatment significantly reduced the Ang II-induced CFs proliferation, α-SMA and collagen expression. In SHR infected with Ad-SCAD, the cardiac fibrosis of the left ventricle was significantly decreased. On the other hand, cardiac fibrosis occurred in conventional SCAD knockout mice. SCAD immunofluorescence intensity of cardiac tissue in hypertensive patients with cardiac fibrosis was lower than that of healthy subjects. All together, the current experimental outcomes indicate that SCAD has a negative regulatory effect on cardiac fibrosis and support its potential therapeutic target for suppressing cardiac fibrosis.

4.
BMC Oral Health ; 24(1): 39, 2024 01 07.
Artigo em Inglês | MEDLINE | ID: mdl-38185621

RESUMO

BACKGROUND: Dentigerous cyst are most common odontogenic cyst and they frequently occur at the mandibular third molar. Their asymptomatic long medical history always resulted in severe bone resorption at the distal aspect of the adjacent second molar. BonMaker® ATB demonstrate an excellent autogenous bone graft candidacy. The aim of this study is to share a single team's experience of dentigerous cyst osseous defect repairing by applying autogenous tooth sticky bone graft. METHOD: In total, 18 patients with dentigerous cyst, which was arised from mandibular third molar unilaterally, were enrolled in this study. Enucleation of dentigerous cyst was performed extracting with involving teeth under general anesthesia. Autogenous tooth sticky bone graft was prepared using extracted tooth and autogenous fibrin glue. Subsequently, grafting was performed above covering with concentrate growth factors. Patients were followed up at sixth months. RESULTS: They were eleven male and seven female patients. Their ages ranged from 20 to 40 years, with a mean of 31 years. Primary wound healing of all sites was achieved in all the patients. Sixth months postoperative radiographic assessment show that dentigerous cysts osseous defects of seventeen patients were good bone filling and ossification. One patient occurred slight bone resorption at the distal aspect of the adjacent second molar. CONCLUSION: Within the limitation of sample size and retrospective nature of the present study, autogenous tooth sticky bone graft demonstrates one of the best alternative alveolar bones repairing graft.


Assuntos
Reabsorção Óssea , Cisto Dentígero , Humanos , Feminino , Masculino , Adulto Jovem , Adulto , Cisto Dentígero/cirurgia , Dente Serotino/cirurgia , Estudos Retrospectivos , Dente Molar
5.
J Cell Mol Med ; 27(24): 3980-3994, 2023 12.
Artigo em Inglês | MEDLINE | ID: mdl-37855260

RESUMO

Co-transplantation of mesenchymal stem cells (MSCs) with telocytes (TCs) was found to have therapeutic effects, although the mechanism of intercellular communication is still unknown. Our current studies aim at exploring the potential molecular mechanisms of TCs interaction and communication with MSCs with a focus on integrin beta1 (ITGB1) in TCs. We found that the co-culture of MSCs with ITGB1-deleted TCs (TCITGB1-ko ) changed the proliferation, differentiation and growth dynamics ability of MSC in responses to LPS or PI3K inhibitor. Changes of MSC proliferation and apoptosis were accompanied with the dysregulation of cytokine mRNA expression in MSCs co-cultured with TCITGB1-ko during the exposure of PI3Kα/δ/ß inhibitor, of which IL-1ß, IL-6 and TNF-α increased, while IFN-γ, IL-4 and IL-10 decreased. The responses of PI3K p85, PI3K p110 and pAKT of MSCs co-cultured with TCITGB1-ko to LPS or PI3K inhibitor were opposite to those with ITGB1-presented TCs. The intraperitoneal injection of TCITGB1-ko , TCvector or MSCs alone, as well as the combination of MSCs with TCITGB1-ko or TCvector exhibited therapeutic effects on LPS-induced acute lung injury. Thus, our data indicate that telocyte ITGB1 contributes to the interaction and intercellular communication between MSCs and TCs, responsible for influencing other cell phenomes and functions.


Assuntos
Lesão Pulmonar Aguda , Células-Tronco Mesenquimais , Telócitos , Humanos , Integrina beta1/genética , Integrina beta1/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Lipopolissacarídeos/farmacologia , Lipopolissacarídeos/metabolismo , Lesão Pulmonar Aguda/terapia , Lesão Pulmonar Aguda/metabolismo , Telócitos/metabolismo , Células-Tronco Mesenquimais/metabolismo , Proliferação de Células , Pulmão/metabolismo
6.
Cytokine ; 171: 156382, 2023 11.
Artigo em Inglês | MEDLINE | ID: mdl-37782985

RESUMO

Globally, tens of millions of individuals experience osteoarthritis (OA), a degenerative joint condition for which a definitive cure is currently lacking. This condition is characterized by joint inflammation and the progressive deterioration of articular cartilage. In this study, western blotting, quantitative reverse-transcription polymerase chain reaction, and immunofluorescence analysis were performed to elucidate the molecular mechanisms by which calcipotriol alleviates chondrocyte ferroptosis. The effect of calcipotriol on reactive oxygen species and lipid peroxidation levels in chondrocytes was assessed using dihydroethidium staining and the fluorescent dye BODIPY. To replicate OA, the destabilized medial meniscus model was employed, followed by the injection of calcipotriol into the knee articular cavity. Morphological analysis was conducted through hematoxylin and eosin staining, safranin O-Fast green staining, and micro-computed tomography analysis. Immunohistochemical analysis was performed to validate the effect of calcipotriol in vivo. Our results demonstrate that the expression of SOX9, col2a1, and Aggrecan, as well as MMP13 and ADAMTS5 protein expression levels, decrease upon treatment with calcipotriol in interleukin-1ß stimulated chondrocytes. Despite these promising outcomes, the exact mechanism underlying calcipotriol's therapeutic effect on OA remains uncertain. We discovered that calcipotriol inhibits chondrocyte GPX4-mediated ferroptosis by suppressing the expression of transforming growth factor-ß1. Furthermore, our study established an in vivo model of OA using rats with medial meniscus instability. Our experiments on rats with OA revealed that intra-articular calcipotriol injection significantly reduces cartilage degradation caused by the disease. Our findings suggest that calcipotriol can mitigate OA by impeding GPX4-mediated ferroptosis of chondrocytes, achieved through the suppression of the TGF-ß1 pathway.


Assuntos
Cartilagem Articular , Ferroptose , Osteoartrite , Ratos , Animais , Condrócitos/metabolismo , Fator de Crescimento Transformador beta1/metabolismo , Osteoartrite/tratamento farmacológico , Osteoartrite/metabolismo , Microtomografia por Raio-X , Células Cultivadas , Cartilagem Articular/metabolismo , Interleucina-1beta/metabolismo
7.
SAGE Open Med Case Rep ; 11: 2050313X231204136, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37799293

RESUMO

Black hairy tongue is a rare condition, characterized by a black discoloration and hair-like structure appearing on the dorsal surface of the tongue. The mechanism of black hairy tongue remains unclear but could be predisposed by multiple factors. We described a case of a 53-year-old Chinese female with a diagnosis of bronchiectasis complicated with Mycobacterium abscessus infection, and a triple antibiotic regimen was prescribed for the infection. One month later, a black hairy tongue appeared. The clinical pharmacist was consulted to investigate the association of medication-related factors by the attending clinician, and the clinical pharmacist identified the potential cause and suggested an appropriate treatment for the black hairy tongue. The black hairy tongue disappeared and did not recur during subsequent treatment.

8.
Neoplasma ; 70(3): 402-415, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-37498068

RESUMO

The regulation of protein kinase B (AKT) phosphorylation by Tripartite motif-containing protein 31 (TRIM31) is implicated as an essential mechanism in the progression of many malignant tumors. Nevertheless, the function of the TRIM31/AKT pathway in oral squamous cell carcinoma (OSCC) remains elusive. Here, immunohistochemistry analysis of human OSCC tissue microarrays indicated significantly higher levels of TRIM31 and phosphorylated AKT (p-AKT) in OSCC tumors than in adjacent tissue samples. Also, we detected a positive association between TRIM31 expression and clinical OSCC development. In in vitro studies, TRIM31 knockdown severely impaired OSCC cell growth, invasion, and migration. By contrast, TRIM31 overexpression improved these cell behaviors, while subsequent AKT inhibition abrogated the effect. In vivo tumorigenesis experiments using nude mice also validated the effects of TRIM31/AKT signaling in tumor growth. Furthermore, TRIM31 upregulation facilitated glucose uptake, as well as lactate and adenosine triphosphate production of OSCC cells, while such positive effects on glycolysis and malignant cell phenotypes were reversed by treatment with AKT or glycolysis inhibitors. In conclusion, TRIM31 may improve OSCC progression by enhancing AKT phosphorylation and subsequent glycolysis. Hence, TRIM31 has the potential as a treatment target in OSCC.


Assuntos
Carcinoma de Células Escamosas , Neoplasias de Cabeça e Pescoço , Neoplasias Bucais , Animais , Humanos , Camundongos , Carcinoma de Células Escamosas/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Glicólise , Camundongos Nus , Neoplasias Bucais/genética , Fosforilação , Proteínas Proto-Oncogênicas c-akt/metabolismo , Carcinoma de Células Escamosas de Cabeça e Pescoço , Proteínas com Motivo Tripartido/metabolismo , Ubiquitina-Proteína Ligases/genética
10.
Cell Biol Toxicol ; 39(6): 2937-2952, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37261679

RESUMO

We present an integrated analysis of the clinical measurements, immune cells, and plasma lipidomics of 2000 individuals representing different age stages. In the study, we explore the interplay of systemic lipids metabolism and circulating immune cells through in-depth analysis of immune cell phenotype and function in peripheral dynamic lipids environment. The population makeup of circulation lymphocytes and lipid metabolites changes dynamically with age. We identified a major shift between young group and middle age group, at which point elevated, immune response is accompanied by the elevation of specific classes of peripheral phospholipids. We tested the effects in mouse model and found that 10-month-dietary added phospholipids induced T-cell senescence. However, the chronic malignant disease, the crosstalk between systemic metabolism and immunity, is completely changed. In cancer patients, the unusual plasma cholesteryl esters emerged, and free fatty acids decreased. The study reveals how immune cell classes and peripheral metabolism coordinate during age acceleration and suggests immune senescence is not isolated, and thus, system effect is the critical point for cell- and function-specific immune-metabolic targeting. • The study identifies a major shift of immune phenotype between young group and middle age group, and the immune response is accompanied by the elevation of specific classes of peripheral phospholipids; • The study suggests potential implications for translational studies such as using metabolic drug to regulate immune activity.


Assuntos
Fosfolipídeos , Exaustão das Células T , Pessoa de Meia-Idade , Camundongos , Animais , Humanos , Fosfolipídeos/análise , Fosfolipídeos/metabolismo , Ácidos Graxos/metabolismo , Ésteres do Colesterol
11.
Artigo em Inglês | MEDLINE | ID: mdl-37126637

RESUMO

Since math word problem (MWP) solving aims to transform natural language problem description into executable solution equations, an MWP solver needs to not only comprehend the real-world narrative described in the problem text but also identify the relationships among the quantifiers and variables implied in the problem and maps them into a reasonable solution equation logic. Recently, although deep learning models have made great progress in MWPs, they ignore the grounding equation logic implied by the problem text. Besides, as we all know, pretrained language models (PLM) have a wealth of knowledge and high-quality semantic representations, which may help solve MWPs, but they have not been explored in the MWP-solving task. To harvest the equation logic and real-world knowledge, we propose a template-based contrastive distillation pretraining (TCDP) approach based on a PLM-based encoder to incorporate mathematical logic knowledge by multiview contrastive learning while retaining rich real-world knowledge and high-quality semantic representation via knowledge distillation. We named the pretrained PLM-based encoder by our approach as MathEncoder. Specifically, the mathematical logic is first summarized by clustering the symbolic solution templates among MWPs and then injected into the deployed PLM-based encoder by conducting supervised contrastive learning based on the symbolic solution templates, which can represent the underlying solving logic in the problems. Meanwhile, the rich knowledge and high-quality semantic representation are retained by distilling them from a well-trained PLM-based teacher encoder into our MathEncoder. To validate the effectiveness of our pretrained MathEncoder, we construct a new solver named MathSolver by replacing the GRU-based encoder with our pretrained MathEncoder in GTS, which is a state-of-the-art MWP solver. The experimental results demonstrate that our method can carry a solver's understanding ability of MWPs to a new stage by outperforming existing state-of-the-art methods on two widely adopted benchmarks Math23K and CM17K. Code will be available at https://github.com/QinJinghui/tcdp.

12.
ACS Omega ; 8(17): 15611-15619, 2023 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-37151497

RESUMO

This paper developed a new methodology for rate transient analysis of fractured wells in carbonate reservoirs. Both the heterogeneity and dual-permeability flow behavior are incorporated into the proposed model, and the fractured carbonate reservoir was simulated with a two-zone composite model. In each zone, a traditional dual-porosity model was applied to describe the characteristics of the natural fractures and matrix. With the Laplace transform, we derived the solution of the mathematical model and plotted new type curves for transient rate decline analysis. Then, the flow regimes were divided and analyzed based on the new type curves. The influences of several critical parameters on transient rate response were also examined. A field case was studied further to demonstrate the precision and application of the proposed method. The results show that the new type curves are mainly composed of eight flow stages. The difference in physical properties (k 2,1, η2,1) between the two zones significantly impacts the transition and boundary-dominated flow regimes. When the values of k 2,1 and η2,1 are smaller, the derivative curve of the transition flow stage will move down, and the duration of this stage on the derivative curve is longer, while the duration of the boundary dominant flow stage will decrease. The dimensionless radial radius of the inner zone (r 1D) can significantly influence the transition flow regime. When r 1D is larger, the production rate and its derivative curve of the transition flow stage will move up, and the duration of this stage will be longer. The results also show that the proposed methodology can effectively fit the field production data. This method can be applied in well productivity evaluation for fractured carbonate reservoirs.

13.
J Proteome Res ; 22(5): 1446-1454, 2023 05 05.
Artigo em Inglês | MEDLINE | ID: mdl-36751022

RESUMO

The global proteome analysis was limited by the identification of peptides with low abundance or specific physiochemical properties. Here, a one-dimensional online alkaline-pH reverse phase nanoelectrospray-tandem mass spectrometry (alkaline-pH-MS/MS) method was developed and optimized for global proteomic analysis. In this method, peptides were separated on a nanoflow C18 column with an alkaline-pH mobile phase (pH = 8.0) and directly injected into the mass spectrometer. The unique peptides overlapped between alkaline-pH-MS/MS and conventional online low-pH reverse phase nanoelectrospray-tandem mass spectrometry (low-pH-MS/MS) were as low as 45%, strongly indicating that these two methods were complementary to each other. In addition, alkaline-pH-MS/MS showed identification capacity for a higher proportion of peptides with negative grand average of hydropathy (GRAVY) or high isoelectric point (pI). Compared to low-pH-MS/MS, alkaline-pH-MS/MS enabled enrichment preference toward histidine-, lysine-, methionine-, and proline-containing peptides. The complementarity of alkaline-pH-MS/MS and low-pH-MS/MS was further demonstrated for the analysis of tryptic digests from 15 intrahepatic cholangiocarcinoma (iCCA) cell lines. The alternating 60 min alkaline-pH-MS/MS plus 60 min low-pH-MS/MS method outperformed the conventional 120 min low-pH-MS/MS method in both the identification of amino acid variants and protein groups. Therefore, we established the alkaline-pH-MS/MS method as a simple, competitive, alternative method to low-pH-MS/MS for global proteomic analysis.


Assuntos
Proteômica , Espectrometria de Massas em Tandem , Espectrometria de Massas em Tandem/métodos , Cromatografia Líquida de Alta Pressão , Proteômica/métodos , Peptídeos/análise , Proteínas do Sistema Complemento , Proteoma/análise , Concentração de Íons de Hidrogênio
14.
Environ Sci Pollut Res Int ; 30(12): 33504-33515, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36480138

RESUMO

Water consumption prediction is an integral part of water resource planning and management. Constructing a highly precise water consumption prediction model is of great significance for promoting regional water resource planning and high-quality development of the socio-economy. This paper focuses on the case of the typical karst region in Guizhou Province in China. Based on data on water consumption and its influencing factors spanning 2000-2020, the principal component analysis method was applied to reduce the dimensionality of 16 influencing factors of water consumption in Guizhou; the principal components extracted were used as input samples of the BP neural network and a PCA-BP neural network water consumption prediction model was conducted to predict water consumption of Guizhou Province in the next 10 years. The results show that the mean absolute error and mean relative error of prediction based on the constructed PCA-BP neural network were 2.8% and 2.9%, respectively, with superior performance in terms of prediction error and trends compared with other models. This paper discusses the main influencing factors of water consumption and analyzes their influence on the water consumption forecasting model so that the parameters of the water consumption forecasting model can be selected more efficiently and provide a reference for regional water consumption analysis and water resource planning and management.


Assuntos
Ingestão de Líquidos , Redes Neurais de Computação , Previsões , China , Análise de Componente Principal
15.
Cytokine ; 162: 156101, 2023 02.
Artigo em Inglês | MEDLINE | ID: mdl-36512936

RESUMO

The goals of present research are to investigate if the genetic polymorphisms in the caspase-1 (CASP1) gene are associated with the risk of rheumatoid arthritis (RA) and the clinical characteristics of the illness in Han patients from China. Our team studied the CASP1 rs2409062 A/G polymorphisms in 1095 healthy controls and 805 RA patients, while the genotype was identified via a custom-by-design 48-Plex single nucleotide polymorphism (SNP) scan™ Kit. The mRNA expression levels of the CASP1 in 40 RA cases and 40 healthy controls were detected by qRT-PCR, while blood plasma levels of the CASP1 in 40 RA cases and 40 paired controls measured via ELISA. Our research showed that the CASP1 rs2409062 A/G polymorphisms were related to an elevated risk for RA. By stratified analysis, our team discovered a remarkably elevated RA risk in females sufferers, age ≥ 55, CRP-positive, or DAS28 < 3.20. In contrast to the control group, the mean level of CASP1 protein in the plasma of RA cases rised significantly. Moreover, RA cases displayed significantly greater levels of CASP1 mRNA versus the control group (P < 0.05). Those outcomes reveal that the CASP1 rs2409062 A/G gene polymorphisms are associated with an elevated risk for RA in a Chinese Han population.


Assuntos
Artrite Reumatoide , Predisposição Genética para Doença , Feminino , Humanos , Predisposição Genética para Doença/genética , População do Leste Asiático , Artrite Reumatoide/genética , Genótipo , Polimorfismo de Nucleotídeo Único/genética , Caspases , China/epidemiologia , Estudos de Casos e Controles , Frequência do Gene
16.
Waste Manag ; 155: 153-161, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36379165

RESUMO

With the rapid expansion of the market scale for indium phosphide (InP) semiconductors in high-tech industries such as optoelectronics and solar energy, the generation of hazardous waste InP has also increased dramatically, and the task of recycling waste InP is urgent. However, InP as a representative phosphide semiconductor is prone to produce highly toxic substances such as yellow phosphorus and PH3 in the recycling process, which discourages most companies from using it. In this study, a safe and efficient method of "vacuum decomposition-directional condensation (VD-DC)" is proposed to recover valuable materials from waste InP. In this method, briquetting pretreatment is used to improve thermal conductivity. At a decomposition temperature of 1123 K, system pressure of 30 Pa, and holding time of 3.5 h, indium with a purity of 99.43 wt% is obtained, and the direct yield reaches 98.54%. Non-toxic and stable red phosphorus with a purity of 98.14 wt% is recovered by converting the condensed yellow phosphorus at 573 K. Vacuum technology significantly reduces the decomposition temperature of InP and avoids the emission of waste water and waste gas, thus operating in an environmentally friendly manner.

17.
Spine J ; 23(2): 271-280, 2023 02.
Artigo em Inglês | MEDLINE | ID: mdl-36252809

RESUMO

BACKGROUND CONTEXT: In recent years, unilateral biportal endoscopic lumbar interbody fusion (ULIF) has been more and more favored by spinal surgeons because of its advantages of low trauma, rapid recovery, high fusion rate and fewer complications. PURPOSE: To compare the clinical effects of ULIF with those of conventional open posterior lumbar interbody fusion (PLIF). STUDY DESIGN: Prospective case control study. PATIENT SAMPLE: Twenty-seven patients treated by ULIF and thirty-three patients treated by PLIF. OUTCOME MEASURES: The preoperative baseline and surgical technique-related outcomes (mean operation time, blood loss during operation, postoperative drainage, and postoperative hospital stay) were compared between the two groups. The clinical status of the two groups before and after surgery were also compared: visual analogue scale (VAS) score of the legs and back, Japanese Orthopedic Association (JOA) score and Oswestry Disability Index (ODI). The clinical laboratory indexes of the two groups before and after the operation were compared: C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), creatine phosphokinase (CPK), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), as well as the incidence of complications, such as dural tear, nerve root injury and infection. METHODS: Adult patients who underwent L3-S1 single level lumbar interbody fusion were included in the study. They were divided into a PLIF group and a ULIF group according to the type of surgery. This study comprised 60 cases: 27 cases in the ULIF group and thirty-three cases in the PLIF group. RESULTS: There was no significant difference in preoperative baseline between the two groups. The ULIF group experienced less blood loss, postoperative drainage and a shorter postoperative hospital stay than the PLIF group; however the ULIF group required a longer operation time than the PLIF group (p<.05). CRP, ESR, CPK, IL-6, and TNF-α levels of the PLIF group were all significantly higher than those of the ULIF group 5 days after surgery (p<.05). The improvements in the VAS scores for back pain, VAS scores for leg pain and JOA score in the ULIF group were all significantly better than those in the PLIF group at 5 days after surgery (p<.05). There was no significant difference in fusion rate at 6 months between the 2 groups (p>.05). CONCLUSIONS: This study showed that ULIF and PLIF were both effective surgical techniques for lumbar interbody fusion. However, ULIF caused less bleeding, reduced inflammatory reaction, less tissue damage and faster postoperative recovery compared with PLIF. Both long-term follow-up and larger clinical studies are needed to validate the clinical and radiological results of this surgery.


Assuntos
Vértebras Lombares , Fusão Vertebral , Adulto , Humanos , Estudos de Casos e Controles , Interleucina-6/sangue , Interleucina-6/química , Vértebras Lombares/cirurgia , Estudos Retrospectivos , Fusão Vertebral/efeitos adversos , Fusão Vertebral/métodos , Resultado do Tratamento , Fator de Necrose Tumoral alfa/sangue , Fator de Necrose Tumoral alfa/química , Proteína C-Reativa/química , Inflamação
18.
Medicine (Baltimore) ; 101(48): e31538, 2022 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-36482553

RESUMO

Locking plate (LP) re-fixation is mainly used to treat postoperative implant periprosthetic refractures; however, the extensive trauma and the fixation form of LP make the operation difficult. The bridge combined fixation system (BCFS) is a new clip-rod internal fixation system, and its clinical application is in its infancy. To compare the clinical effect of BCFS and LP in the treatment of geriatric postoperative implant periprosthetic refracture following proximal femoral fracture surgery. Thirty-two patients (14 with BCFS and 18 with LP) with postoperative implant periprosthetic refracture following proximal femoral fracture surgery, who underwent surgery in our hospital, were analyzed retrospectively. The incision length, operation time, intraoperative bleeding volume, postoperative drainage volume, postoperative hospital stay, fracture healing time and complications of each patient were recorded. Regular radiographs were taken after the operation to evaluate the fracture reduction and fixation. All the patients were followed for 12 months to evaluate their limb function by Johner-Wruhs scoring criteria. The patients were followed for an average of 24.1 months, and all achieved bony union, with no complications such as infection, nonunion, and internal fixation instrument falling off and loosening after the operation. Delayed healing occurred in two cases in the LP group. The average value of surgical incision length, operation time, postoperative hospitalization time and fracture healing time in the BCFS group were significantly smaller than those in the LP group, accompanied by a decrease in intraoperative bleeding and postoperative drainage volumes (P < .05). The rate of limb function in the BCFS group (85.7%) was higher than that in the LP group (83.3%), with no significance (P > .05). The BCFS in the refracture around the implant of the proximal femoral fracture exhibited many advantages such as simple operation, strong plasticity, effective reduction of surgical trauma, promotion of fracture healing and early functional rehabilitation, etc, making it an advantageous clinical application.


Assuntos
Fraturas Proximais do Fêmur , Humanos , Idoso , Estudos Retrospectivos
19.
BMC Oral Health ; 22(1): 433, 2022 10 02.
Artigo em Inglês | MEDLINE | ID: mdl-36184595

RESUMO

BACKGROUND: Impaction of mandibular third molars (M3) is one of the most common diseases. Extraction of M3 usually exacerbates osseous defects at the distal aspect of the adjacent second molar (M2). BonMaker® ATB has been cited as a novel autogenous bone grafting material. The aim of this pilot study was to introduce a novel method for repairing the distal osseous defects of M2 after the surgical removal of M3 with autogenous tooth graft powder (ATGP). METHOD: A total of five patients were enrolled in this prospective split-mouth clinical pilot study. Four impacted wisdom teeth were extracted bilaterally from each patient with proximal alveolar bone loss ≥ 5 mm of M3. The ATGP was prepared chairside from two extracted one side third molars and randomly implanted in one of the M3 extraction sockets, and the other side was treated with a blank and considered the control site. Patients were followed up at 6 months. RESULTS: The five patients included three males and two females. Their ages ranged from 25 to 30 years, with a median of 27 years. Primary wound healing without complications was achieved in all the patients. There was a greater tendency for swelling of the cheeks and trismus to occur at the experimental site on the third postoperative day. Compared with the control site, the experimental site exhibited progressive bone filling and ossification in the sixth postoperative month. Moreover, the probing pocket depth of the experimental site was lower than that of the control site. CONCLUSION: The results of this study demonstrate that ATGP effectively and economically repairs distal osseous defects of M2. Further study is required to validate the effectiveness with a larger study population.


Assuntos
Dente Serotino , Dente Impactado , Adulto , Transplante Ósseo/métodos , Feminino , Humanos , Masculino , Mandíbula/cirurgia , Dente Molar , Dente Serotino/cirurgia , Projetos Piloto , Pós , Estudos Prospectivos , Extração Dentária/efeitos adversos , Extração Dentária/métodos , Dente Impactado/cirurgia
20.
Comput Intell Neurosci ; 2022: 5207178, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36120698

RESUMO

To realize the automatic symptom recognition and classification of MR images and improve the accuracy and efficiency of the diagnosis of lumbar intervertebral disc herniation (LDH), a method for lumbar intervertebral disc recognition and disease classification is proposed in this paper. The method mainly includes three steps: preprocessing, target segmentation, and symptom classification. Preprocessing is performed by noise reduction and interference removal methods for blurred images. The contour poles are used to determine the four points of the tail vertebra in order to reduce the wrong segmentation of the tail vertebra. A classification method based on five judgment indicators is proposed, which effectively improves the stability of disease diagnosis. The example verifies that the algorithm can accurately complete the target segmentation and the accuracy of symptom classification reaches the standard of professional doctors, which proves that the method has good robustness.


Assuntos
Processamento de Imagem Assistida por Computador , Imageamento por Ressonância Magnética , Algoritmos , Processamento de Imagem Assistida por Computador/métodos , Imageamento por Ressonância Magnética/métodos
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