Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 35
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Acta Pharmacol Sin ; 2021 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-34234269

RESUMO

Phage display technology allows for rapid selection of antibodies from the large repertoire of human antibody fragments displayed on phages. However, antibody fragments should be converted to IgG for biological characterizations and affinity of antibodies obtained from phage display library is frequently not sufficient for efficient use in clinical settings. Here, we describe a new approach that combines phage and mammalian cell display, enabling simultaneous affinity screening of full-length IgG antibodies. Using this strategy, we successfully obtained a novel germline-like anti-TIM-3 monoclonal antibody named m101, which was revealed to be a potent anti-TIM-3 therapeutic monoclonal antibody via in vitro and in vivo experiments, indicating its effectiveness and power. Thus, this platform can help develop new monoclonal antibody therapeutics with high affinity and low immunogenicity.

3.
Appl Microbiol Biotechnol ; 104(1): 107-117, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31734810

RESUMO

Vaccination has been regarded as the most effective way to reduce death and morbidity caused by infectious diseases in the livestock industry. In this study, plasma activated water (PAW) was introduced to prepare the inactivated Newcastle disease vaccine. Humoral immune response was tested by hemagglutination inhibition (HI) assay and enzyme-linked immunosorbent assay (ELISA). In addition, cell-mediated immune response was evaluated by lymphocyte proliferation assay and flow cytometry. The results demonstrated that the vaccine prepared by PAW at appropriate volume ratio could induce similar antibody titers in specific pathogen-free (SPF) chickens compared with the formaldehyde-inactivated vaccine. The challenge experiment further confirmed that the vaccine prepared by PAW conferred solid protection against virulent NDV. Moreover, it was found that the vaccine could promote the proliferation of lymphocytes and stimulate cell-mediated immunity of SPF chickens. Furthermore, analysis of electron spin resonance (ESR) spectroscopy and physicochemical properties of PAW suggested reactive oxygen and nitrogen species (RONS) played an essential role in the virus inactivation. Therefore, this study indicated that NDV treated by PAW in an appropriate ratio retained immunogenicity on the premise of virus inactivation. PAW as a promising strategy could be used to prepare inactivated vaccine for Newcastle disease.


Assuntos
Doença de Newcastle/prevenção & controle , Vírus da Doença de Newcastle/imunologia , Gases em Plasma/química , Vacinas Virais/imunologia , Água/análise , Animais , Anticorpos Antivirais/sangue , Galinhas/imunologia , Testes de Inibição da Hemaglutinação , Imunidade Celular , Imunidade Humoral , Ativação Linfocitária , Doenças das Aves Domésticas/prevenção & controle , Doenças das Aves Domésticas/virologia , Organismos Livres de Patógenos Específicos , Vacinas de Produtos Inativados , Vacinas Virais/administração & dosagem
4.
Nat Prod Res ; 34(6): 847-850, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30445844

RESUMO

Thirty-three compounds (1-33) were isolated from the rhizomes of Osmunda japonica. Their structures were elucidated on the basis of spectral analysis and identified as ent-kaurene terpenoids (1-3), anthraquinones (4-8), flavonoids (9-12), steroids (13-15), and other compounds (16-33). Compound 1-14, 19-27 were isolated from the genus osmunda for the first time. Compound 28-29 were isolated from the plant for the first time. Cytotoxicities of all compounds against Hela, HepG2 and A549 cell lines were measured by MTT assay, and their antioxidant activities were determined by DPPH and ABTS radical scavenging assays. Compound 2 exhibited potent cytotoxicity against Hela, HepG2 cell lines with IC50 values of 9.31 and 9.66 µM, respectively. Compound 9 showed good antioxidant activity. The Trolox-equivalent antioxidant capacity (TEAC) value was 0.95 mM in ABTS assay, and the IC50 value was 18.63 µM in DPPH assay.


Assuntos
Antineoplásicos/isolamento & purificação , Antioxidantes/isolamento & purificação , Extratos Vegetais/química , Rizoma/química , Traqueófitas/química , Antraquinonas/química , Antraquinonas/isolamento & purificação , Antineoplásicos/química , Antioxidantes/química , Antioxidantes/farmacologia , Linhagem Celular Tumoral , Flavonoides/química , Flavonoides/isolamento & purificação , Humanos , Extratos Vegetais/farmacologia , Terpenos/química , Terpenos/isolamento & purificação
5.
Chin J Integr Med ; 25(11): 812-819, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31471834

RESUMO

OBJECTIVE: To evaluate the association between Chinese medicine (CM) therapy and disease-free survival (DFS) outcomes in postoperative patients with non-small cell lung cancer (NSCLC). METHODS: This multiple-center prospective cohort study was conducted in 13 medical centers in China. Patients with stage I, II, or IIIA NSCLC who had undergone radical resection and received conventional postoperative treatment according to the National Comprehensive Cancer Network (NCCN) guidelines were recruited. The recruited patients were divided into a CM treatment group and a control group according to their wishes. Patients in the CM treatment group received continuous CM therapy for more than 6 months or until disease progression. Patients in the control group received CM therapy for less than 1 month. Follow-up was conducted over 3 years. The primary outcome was DFS, with recurrence/metastasis rates as a secondary outcome. RESULTS: Between May 2013 and August 2016, 503 patients were enrolled into the cohort; 266 were classified in the CM treatment group and 237 in the control group. Adjusting for covariates, high exposure to CM was associated with better DFS [hazard ratio (HR) = 0.417, 95% confidential interval (CI): 0.307-0.567)]. A longer duration of CM therapy (6-12 months, 12-18 months, >24 months) was associated with lower recurrence and metastasis rates (HR = 0.225, 0.119 and 0.083, respectively). In a subgroup exploratory analysis, CM therapy was also a protective factor of cancer recurrence and metastasis in both stage I-IIIA (HR=0.50, 95% CI: 0.37-0.67) and stage IIIA NSCLC postoperative patients (HR = 0.48, 95% CI: 0.33-0.71), DFS was even longer among CM treatment group patients. CONCLUSIONS: Longer duration of CM therapy could be considered a protective factor of cancer recurrence and metastasis. CM treatment is associated with improving survival outcomes of postoperative NSCLC patients in China. (Registration No. ChiCTR-OOC-14005398).


Assuntos
Carcinoma Pulmonar de Células não Pequenas/terapia , Neoplasias Pulmonares/terapia , Medicina Tradicional Chinesa , Cuidados Pós-Operatórios/métodos , Adulto , Idoso , Carcinoma Pulmonar de Células não Pequenas/epidemiologia , Carcinoma Pulmonar de Células não Pequenas/cirurgia , China/epidemiologia , Estudos de Coortes , Terapia Combinada/estatística & dados numéricos , Intervalo Livre de Doença , Feminino , Seguimentos , Humanos , Neoplasias Pulmonares/epidemiologia , Neoplasias Pulmonares/cirurgia , Masculino , Medicina Tradicional Chinesa/métodos , Medicina Tradicional Chinesa/estatística & dados numéricos , Pessoa de Meia-Idade , Cuidados Pós-Operatórios/estatística & dados numéricos , Período Pós-Operatório , Resultado do Tratamento
6.
Appl Opt ; 57(24): 7070-7075, 2018 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-30129601

RESUMO

We present the results of numerical simulation of a dissipative solitons mode-locked fiber laser working over 1.6 µm. First, systematic and computationally intensive analysis of high-energy-pulse nonlinear phase shift of a fiber laser was conducted, and we numerically simulated dissipative solitons pulse evolution. In addition, we employed a method named A/B ratio to decrease B-integral by changing the distribution of passive fiber in the ring cavity. This method inhibited the accumulation of nonlinear phase shift and effectively enlarged the pulse energy from 0.8 nJ to 3.6 nJ. Finally, we optimized linear chirp of the pulse by using a designed larger-mode-area fiber, and ultimately a 2.82-kW, 650-fs pulse was obtained.

7.
Huan Jing Ke Xue ; 39(1): 321-330, 2018 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965698

RESUMO

Controlling agricultural greenhouse gas emissions, such as N2O, is important in mitigating global climate warming. Through monitoring the dynamics of N2O emission fluxes, we investigated the effect of organic nitrogen (N) substitution of synthetic N on N2O emissions and the yield of winter wheat and summer maize in the Guanzhong Plain of Shaanxi Province, China. The study involved six treatments, consisting of no fertilizer (CK), synthetic N, phosphorus (P), and potassium (K) fertilizers alone (NPK), 75% NPK+25% organic N through manure (25%M), 50% NPK+50% organic N (50%M), 25% NPK+75% organic N (75%M), 100% organic N (100%M). The results showed that the peak value of the N2O emission flux appeared after fertilization, rainfall, and irrigation. In the wheat season, the emission flux of N2O varied from -1.33 to 144.2 µg·(m2·h)-1, with the highest peak value in the NPK treatment. In the maize season, the emission flux of N2O varied from 88.2 to 1800.1 µg·(m2·h)-1, and the 50%M treatment showed the highest peak value. The range in the total amount of N2O emissions from the different treatments in the wheat-maize rotation system was 429.8-2632.1 g·hm-2, and the amount for the treatments decreased in order as follows:50%M > 25%M > NPK > 75%M > 100%M > CK. The yields of wheat, maize, or wheat plus maize were significantly higher in the fertilized treatments compared to the CK. Organic substitution treatments significantly increased wheat yield by 26.1% to 50.0% relative to the NPK treatment. While the maize yield in 50%M and 75%M treatments was similar to that in the NPK treatment, the 25%M and 100%M treatments showed significantly lower yields than with the NPK treatment. The total yield of wheat plus maize varied from 9166 to 17496 kg·hm-2, of which total yield was significantly higher with 50%M and 75%M compared to NPK. Overall, the 75%M treatment is the best measure to guarantee crop yield and to reduce N2O emissions in the wheat-maize rotation system based on a one year study in the Guanzhong plain of Shaanxi Province.


Assuntos
Agricultura/métodos , Fertilizantes , Esterco , Nitrogênio/química , China , Gases de Efeito Estufa/análise , Óxido Nitroso/análise , Solo , Triticum , Zea mays
8.
Am Surg ; 84(2): 215-219, 2018 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-29580348

RESUMO

The aim of this study was to determine the feasibility of prosthetic mesh repair according to the degree of bowel necrosis in the emergency management of acutely strangulated groin hernias. Emergency prosthetic mesh repair versus primary suture repair was randomly performed in 208 consecutive strangulated groin hernia patients with bowel necrosis between January 2005 and August 2016. The degree of bowel necrosis of each patient was determined according to a modified three-grade classification system. Patient characteristics sorted by repair method were analyzed by using Pearson's chi-squared tests. Correlations between mortality and wound-related morbidity with bowel necrosis grade and repair method were analyzed. There was no difference in gender, age, body mass index, comorbid diseases, hernia type (left or right, primary or recurrent), necrosis grade, and mortality between the mesh repair and suture repair groups (all P > 0.05). However, with regard to wound-related morbidity, there was significant difference between the two groups (P < 0.05). Mortality and wound-related morbidity showed significant relationship with necrosis grade, especially with regard to postoperative wound infection (P < 0.001). The wound infection rate with mesh repair was significantly higher than that with primary suture in Grade II and III necrosis patients (P < 0.05), but there was no difference in Grade I patients (P > 0.05). The use of prosthetic mesh in the emergency repair of acutely strangulated groin hernias seems to be as safe as suture-only repair in patients with noninfected strangulated bowel (Grade I necrosis). The use of prosthetic mesh repair is a rational choice made based on the degree of bowel necrosis in the emergency management of acutely strangulated hernias.


Assuntos
Hérnia Inguinal/cirurgia , Herniorrafia/métodos , Intestinos/patologia , Telas Cirúrgicas , Adulto , Idoso , Idoso de 80 Anos ou mais , Emergências , Estudos de Viabilidade , Feminino , Hérnia Inguinal/complicações , Hérnia Inguinal/patologia , Herniorrafia/instrumentação , Humanos , Masculino , Pessoa de Meia-Idade , Necrose , Técnicas de Sutura , Resultado do Tratamento
9.
J Med Virol ; 89(11): 1988-1994, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-28577316

RESUMO

Hepatitis E virus (HEV) infection is a serious public health concern in developing countries. China is regarded as an HEV-endemic area, but epidemiological data for HEV among different nationalities is limited. This study was conducted to estimate the seroprevalence and risk factors of HEV infection in Koreans (n = 520), Manchus (n = 303), Mongols (n = 217), and Hans (n = 802) in Eastern and Northeastern China between 2013 and 2015. A total of 366 (19.87%) out of 1842 samples were seropositive for IgG or IgM HEV-antibodies detected by enzyme-linked immunoassays. Among these groups, the Mongols had the highest seroprevalence of HEV infection (25.35%, 55/217), followed by the Koreans (23.65%, 123/520), the Manchus (19.80%, 60/303), and the Hans (15.96%, 128/802). Multiple analysis showed that the gender, consumption of raw/undercooked meat, source of drinking water, residence area, and age were significantly associated with HEV infection in four ethnic groups. The present results indicated that HEV infection was prevalent in Mongols, Koreans, Manchus, and Hans in the surveyed regions, which demonstrated the higher risk of transmitting HEV in multiple nationalities in Eastern and Northeastern China.


Assuntos
Anticorpos Anti-Hepatite/sangue , Vírus da Hepatite E/imunologia , Hepatite E/etnologia , Estudos Soroepidemiológicos , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , China/epidemiologia , Ensaio de Imunoadsorção Enzimática , Feminino , Hepatite E/imunologia , Hepatite E/virologia , Vírus da Hepatite E/isolamento & purificação , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Prevalência , Fatores de Risco , Adulto Jovem
10.
Biomed Res Int ; 2017: 5139703, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28512638

RESUMO

Bluetongue (BT), caused by bluetongue virus (BTV), is an arthropod-borne viral disease in ruminants. However, information about BTV infection in yaks in China is limited. Moreover, no such data concerning BTV in Tibetan sheep is available. Therefore, 3771 serum samples were collected from 2187 Tibetan sheep and 1584 yaks between April 2013 and March 2014 from Tibetan Plateau, western China, and tested for BTV antibodies using a commercially available ELISA kit. The overall seroprevalence of BTV was 17.34% (654/3771), with 20.3% (443/2187) in Tibetan sheep and 13.3% (211/1584) in yaks. In the Tibetan sheep group, the seroprevalence of BTV in Luqu, Maqu, Tianzhu, and Nyingchi Prefecture was 20.3%, 20.8%, 20.5%, and 19.1%, respectively. The seroprevalence of BTV in different season groups varied from 16.5% to 23.4%. In the yak group, BTV seroprevalence was 12.6%, 15.5%, and 11.0% in Tianzhu, Maqu, and Luqu counties, respectively. The seroprevalence in different seasons was 12.6%, 15.5%, 15.4%, and 9.0% in spring, summer, autumn, and winter, respectively. The season was the major risk factor concerning BTV infection in yaks (P < 0.05). The date of the BTV seroprevalence in Tibetan sheep and yaks provides baseline information for controlling BT in ruminants in western China.


Assuntos
Vírus Bluetongue/imunologia , Bluetongue/epidemiologia , Bluetongue/etiologia , Ovinos/virologia , Animais , Anticorpos Antivirais/sangue , Anticorpos Antivirais/imunologia , Bluetongue/sangue , Bluetongue/virologia , Bovinos , China/epidemiologia , Fatores de Risco , Estudos Soroepidemiológicos , Ovinos/sangue , Tibet/epidemiologia
11.
Huan Jing Ke Xue ; 38(6): 2586-2593, 2017 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965381

RESUMO

The primary aim of this study was to quantify the effects of long-term organic amendments on soil nitrous oxide (N2O) emissions. Using static chamber-gas chromatograph technique, we measured N2O fluxes from winter wheat-maize rotation system and related environmental factors in the Guanzhong Plain for one year (October 2014 to October 2015). Field experiments were based on the "Chinese National Loess Fertility and Fertilizer Effects Long-term Monitoring Experiment". Four treatments were control (CK, 0 kg·hm-2), NPK (NPK, 353 kg·hm-2), NPK combined with maize straw[NPKS, (353+40) kg·hm-2] and cattle waste[NPKM, (238+115) kg·hm-2]. During the experimental period, N2O fluxes from CK treatment were small[<2.9 g·(hm2·d)-1]; while emissions from fertilized treatments peaked after fertilization[up to 113.4 g·(hm2·d)-1 for NPKS] and irrigation[up to 495.0 g·(hm2·d)-1 for NPKM] during winter wheat and maize seasons, respectively. N2O flux was significantly correlated to soil water-filled pore space for all treatments (r>0.28,P<0.05). Annual N2O emissions were (0.1±0.0), (2.6±0.1), (3.4±0.7) and (2.9±0.3) kg·hm-2 for CK, NPK, NPKS and NPKM, respectively. The fertilized treatments released higher N2O emissions than CK treatment (P<0.05), indicating that fertilization stimulated N2O emissions. However, the differences in N2O emissions were not significant among the fertilized treatments (P=0.06), suggesting that organic amendments did not increase N2O emissions obviously. The direct emission factors were 0.72%, 0.83% and 0.80% for NPK, NPKS and NPKM, respectively, all of which were lower than the IPCC default of 1%. The yield-scaled N2O emission for NPKM was the lowest among the fertilized treatments.


Assuntos
Agricultura , Fertilizantes , Óxido Nitroso/análise , Triticum/crescimento & desenvolvimento , Zea mays/crescimento & desenvolvimento , Animais , Bovinos , China , Solo/química
12.
Parasitol Res ; 114(12): 4725-9, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26403980

RESUMO

Toxoplasma gondii is a zoonotic protozoan of almost all species of mammals and birds. However, no information is available about seroprevalence of the pathogen in stray dogs in seven cities from Inner Mongolia, Jilin, Liaoning province, China. Using the indirect hemagglutination antibody (IHA) test with a cutoff titer of 1:64, 129 (14.05 %) out of 918 stray dogs were surveyed to be T. gondii seropositive. Analysis of the risk factor showed that there was no statistical difference of seroprevalence of T. gondii between males (13.46 %) and females (14.54 %) (P > 0.05). The seropositive rates of T. gondii infection in stray dogs among the seven cities in northern China were significant statistically different (P = 0.005). Moreover, seroprevalence of T. gondii in dogs were varied from 11.01 % (95 % CI 6.94-15.09) to 16.86 % (95 % CI 12.27-21.46) among different age groups, and the difference was statistically significant (P = 0.03). More importantly, T. gondii seropositive in stray dogs has a declining tendence from 2011 to 2015 (P = 0.004). The results of the present study showed that stray dogs in Inner Mongolia, Jilin, Liaoning province, China are exposed to T. gondii, for the first time, and could also provide a foundation data for prevent and control T. gondii infection in dogs, other animals and humans.


Assuntos
Anticorpos Antiprotozoários/sangue , Doenças do Cão/parasitologia , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/parasitologia , Animais , China/epidemiologia , Cidades , Doenças do Cão/sangue , Doenças do Cão/diagnóstico , Doenças do Cão/epidemiologia , Cães , Feminino , Testes de Hemaglutinação , Humanos , Masculino , Fatores de Risco , Estudos Soroepidemiológicos , Toxoplasma/imunologia , Toxoplasmose Animal/sangue , Toxoplasmose Animal/diagnóstico , Toxoplasmose Animal/epidemiologia
13.
Nutr Res Pract ; 9(1): 30-6, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25671065

RESUMO

BACKGROUD/OBEJECTIVES: The mechanism of how black garlic effects lipid metabolism remains unsolved. Therefore, the objectives of this study were to determine the effects of black garlic on lipid profiles and the expression of related genes in rats fed a high fat diet. MATERIALS/METHODS: Thirty-two male Sqrague-Dawley rats aged 4 weeks were randomly divided into four groups (n=8) and fed the following diets for 5 weeks: normal food diet, (NF); a high-fat diet (HF); and a high-fat diet + 0.5% or 1.5% black garlic extract (HFBG0.5 or HFBG1.5). Body weights and blood biochemical parameters, including lipid profiles, and expressions of genes related to lipid metabolism were determined. RESULTS: Significant differences were observed in the final weights between the HFBG1.5 and HF groups. All blood biochemical parameters measured in the HFBG1.5 group showed significantly lower values than those in the HF group. Significant improvements of the plasama lipid profiles as well as fecal excretions of total lipids and triglyceride (TG) were also observed in the HFBG1.5 group, when compared to the HF diet group. There were significant differences in the levels of mRNA of sterol regulatory element binding protein-1c (SREBP-1c), acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), and glucose-6-phosphate dehydrogenase (G6PDH) in the HFBG1.5 group compared to the HF group. In addition, the hepatic expression of (HMG-CoA) reductase and Acyl-CoA cholesterol acyltransferase (ACAT) mRNA was also significantly lower than the HF group. CONCLUSIONS: Consumption of black garlic extract lowers SREBP-1C mRNA expression, which causes downregulation of lipid and cholestrol metahbolism. As a result, the blood levels of total lipids, TG, and cholesterol were decreased.

14.
Int J Biol Sci ; 10(10): 1138-49, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25332688

RESUMO

HIV-1 tat targets a variety of host cell proteins to facilitate viral transcription and disrupts host cellular immunity by inducing lymphocyte apoptosis, but whether it influences humoral immunity remains unclear. Previously, our group demonstrated that tat depresses expression of DNA-PKcs, a critical component of the non-homologous end joining pathway (NHEJ) of DNA double-strand breaks repair, immunoglobulin class switch recombination (CSR) and V(D)J recombination, and sensitizes cells to ionizing radiation. In this study, we demonstrated that HIV-1 Tat down-regulates DNA-PKcs expression by directly binding to the core promoter sequence. In addition, Tat interacts with and activates the kinase activity of DNA-PKcs in a dose-dependent and DNA independent manner. Furthermore, Tat inhibits class switch recombination (CSR) at low concentrations (≤ 4 µg/ml) and stimulates CSR at high concentrations (≥ 8 µg/ml). On the other hand, low protein level and high kinase activity of DNA-PKcs promotes HIV-1 transcription, while high protein level and low kinase activity inhibit HIV-1 transcription. Co-immunoprecipitation results revealed that DNA-PKcs forms a large complex comprised of Cyclin T1, CDK9 and Tat via direct interacting with CDK9 and Tat but not Cyclin T1. Taken together, our results provide new clues that Tat regulates host humoral immunity via both transcriptional depression and kinase activation of DNA-PKcs. We also raise the possibility that inhibitors and interventions directed towards DNA-PKcs may inhibit HIV-1 transcription in AIDS patients.


Assuntos
Proteína Quinase Ativada por DNA/metabolismo , Regulação Viral da Expressão Gênica/fisiologia , HIV-1/metabolismo , Imunidade Humoral/imunologia , Switching de Imunoglobulina/fisiologia , Transcrição Genética/genética , Produtos do Gene tat do Vírus da Imunodeficiência Humana/metabolismo , Primers do DNA/genética , Ensaio de Desvio de Mobilidade Eletroforética , Citometria de Fluxo , Regulação Viral da Expressão Gênica/genética , Células HEK293 , HIV-1/genética , Células HeLa , Humanos , Imunoprecipitação , Luciferases , Linfócitos T , Produtos do Gene tat do Vírus da Imunodeficiência Humana/imunologia
15.
Vector Borne Zoonotic Dis ; 14(6): 394-8, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24866015

RESUMO

Toxoplasmosis, caused by the obligate intracellular protozoan parasite Toxoplasma gondii, has become a serious public health problem worldwide. T. gondii can infect almost all warm-blooded animals, including parrots. However, little is known of T. gondii infection in parrots in China. Antibodies against T. gondii in 311 parrots including 202 Budgerigars (Melopsittacus undulatus), 26 Lovebirds (Agapornis sp.), 22 Cockatiels (Nymphicus hollandicus), and 61 Alexandrine Parakeets (Psittacula eupatria) in the cities of Beijing and Weifang in north China were tested using the modified agglutination test (MAT). Twenty-six (8.36%) out of 311 serum samples were positive for T. gondii at the cutoff of 1:5. Among the four species, a higher seroprevalence of T. gondii was found in Cockatiels (13.64%, 95% confidence interval [CI] 0.00-27.98), although the difference was not statistically significant (p=0.61). Seropositivity rates against T. gondii in male parrots (10.43%, 95% CI 5.74-15.12) were not statistically different from that in female parrots (6.08%, 95% CI 2.23-9.93, p=0.17). The seropositivity of T. gondii in parrots from Weifang and Beijing was 11.11% (95% CI 6.13-16.09) and 5.70% (95% CI 2.08-9.31), respectively. The seroprevalence varied in parrots of different age groups, ranging from 5.71% (95% CI 1.27-10.15) to 13.00% (95% CI 6.41-19.69), however, the difference among age groups was not statistically significant (p=0.12). The seroprevalence of T. gondii infection in parrots in summer (11.63%, 95% CI 6.84-16.42) was significantly higher than in spring (4.32%, 95% CI 0.94-7.70, p=0.02). The results of the present survey indicated that parrots in China are exposed to T. gondii. To our knowledge, this is the first report of T. gondii seroprevalence in parrots in China.


Assuntos
Testes de Aglutinação/veterinária , Anticorpos Antiprotozoários/sangue , Toxoplasma/imunologia , Toxoplasmose Animal/epidemiologia , Animais , China/epidemiologia , Feminino , Masculino , Papagaios , Estudos Soroepidemiológicos , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/parasitologia
16.
Environ Sci Technol ; 46(5): 2911-7, 2012 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-22304368

RESUMO

A large amount of organophosphate pesticides (OPs) is used throughout China. Animal studies have suggested that even moderate doses are toxic to neurodevelopment, but there are a few studies in humans. We investigated both the urinary levels of OP metabolites in children and their relationship with child neurodevelopment. Participating 301 young children (23-25 months of age) were recruited from two community hospitals in Shanghai between February and October 2008. We measured five nonspecific dialkyl phosphate (DAP) metabolite levels of OPs in the children's urine and examined their association with the children's developmental quotients (DQs) based on the Gesell Developmental Schedules (GDS). The creatinine-adjusted geometric means (GMs) of OP metabolites in urine samples were 11.27 µg/g for DMP; 6.99 µg/g for DMTP; 7.96 µg/g for DEP; 14.19 µg/g for DETP; and 4.55 µg/g for DEDTP. The children had relatively higher levels of OP urinary metabolites compared with those reported in developed countries, no association was found between child urinary levels of OP metabolites and any of the DQ scores. However, our results should be interpreted with caution, and more studies of children living in China are warranted given the relatively high levels of child OP urinary metabolites in Shanghai.


Assuntos
Exposição Ambiental/análise , Sistema Nervoso/crescimento & desenvolvimento , Compostos Organofosforados/urina , Praguicidas/urina , Criança , Desenvolvimento Infantil , Pré-Escolar , China , Creatinina/metabolismo , Demografia , Monitoramento Ambiental , Feminino , Humanos , Idioma , Masculino , Atividade Motora/fisiologia
17.
Zhonghua Yi Xue Za Zhi ; 92(48): 3426-8, 2012 Dec 25.
Artigo em Chinês | MEDLINE | ID: mdl-23327705

RESUMO

OBJECTIVE: To explore the clinical characteristic and therapeutic strategies of acute appendicitis after radical gastrectomy for gastric carcinoma. METHODS: The clinical data of 31 patients with acute appendicitis after radical gastrectomy for gastric carcinoma from January 2006 to January 2012 was analyzed retrospectively. The profiles of previous operations, symptoms, physical signs, disease duration, progression time, examination results, peri-operative complications, results of bacterial culture and use of antibiotics were used to evaluate the clinical characteristics and therapeutic strategies. RESULTS: There were 19 males and 12 females with a mean age of (61 ± 4) years. Gastric cancer postoperative acute appendicitis lacked typical symptoms. The presenting symptoms were persistent and progressive severe right lower abdominal pains (n = 31, 100.0%), associated, with fever (n = 27, 87.1%) nausea or vomiting (n = 11, 35.5%), abdominal distension (n = 9, 29.0%), intestinal obstruction (n = 21, 67.7%) and abdominal purulent exudate (n = 31, 100.0%). The average onset time from abdominal pain to peritonitis was (15 ± 4) hours. Perforated appendix occurred in 16 cases (51.6%). Seven patients had no increase of the total number of WBC or percentage of neutrophils (22.6%). Exploratory laparotomy was performed in 17 cases, and the rate of delayed diagnosis was 54.8%. And 31 patients were cured by surgery and anti-infection treatment. There was no intraoperative death. CONCLUSIONS: Because of rapidly spreading abdominal infection, peritonitis occurs early with a high incidence rate. Early diagnosis, early operation and rational use of antibiotics are the most important therapeutic modalities of acute postoperative appendicitis in patients with gastric cancer.


Assuntos
Apendicite/diagnóstico , Apendicite/etiologia , Gastrectomia/efeitos adversos , Complicações Pós-Operatórias , Neoplasias Gástricas/cirurgia , Doença Aguda , Idoso , Apendicite/tratamento farmacológico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
18.
Ying Yong Sheng Tai Xue Bao ; 22(3): 621-30, 2011 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-21657016

RESUMO

By using GLOPEM-CEVSA model, the spatiotemporal pattern and its affecting factors of the vegetation net primary productivity (NPP) in Northeast China in 2000-2008 were simulated, and, taking four forest ecosystem stations (Daxing' anling, Laoyeling, Liangshui and Changbai Mountains) as the cases, the seasonal changes and their main driving force of forest NPP in Northeast China were studied. In 2000-2008, the annual averaged vegetation NPP in the region was 445 g C x m(-2) x a(-1), being the highest in the areas from Changbai Mountains to Xiaoxing' anling Mountains and parts of Sanjiang Plain, followed by in the areas from Changbai Mountains to Liaohe River Plain, eastern Songnen Plain, Sanjiang Plain, and Daxing' anling Mountain, and the lowest in the sparse grass and desert areas in the west. Forest ecosystem had the highest annual averaged NPP, followed by shrub, cropland and grassland, and desert. In forest ecosystem, coniferous and broad-leaf mixed forests had the highest annual averaged NPP (722 g C x m(-2) x a(-1)), while deciduous needle-leaf forest had the lowest one (451 g C x m(-2) x a(-1)). During the study period, no significant inter-annual changes were observed in the forest NPP though it was higher in 2007 and 2008 probably due to the increased air temperature (1 degrees C-2 degrees C higher than that in other years). The beginning time of forest growth season in Northeast China advanced gradually from north to south, and the growth season became longer.


Assuntos
Biomassa , Ecossistema , Monitoramento Ambiental/métodos , Árvores/crescimento & desenvolvimento , China , Simulação por Computador , Geologia , Estações do Ano
19.
Pediatr Surg Int ; 27(11): 1203-12, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21691763

RESUMO

PURPOSE: Kidney ischemia-reperfusion injury (IRI) after kidney transplant remains a major problem, separate from immune rejection that can lead to kidney transplant failure and graft function loss. Free radicals, disturbance of microcirculation and the inflammatory cascade appear to be the main contributors. Radix Codonopsis, a traditional Chinese drug used in vascular diseases, is an antioxidant and free radical scavenger. This study investigates the protective effect and underlying mechanisms of Radix Codonopsis extract saponins on kidney transplantation. METHODS: Renal transplantation was performed after rat kidneys had been stored for 1 h at 4°C. Blood urea nitrogen (BUN), serum creatinine (Scr), superoxide dismutase (SOD) and malondialdehyde (MDA) were assayed; bcl-2 and bax mRNA expression was detected using RT-PCR; bcl-2 and bax protein expression was detected by immunohistochemistry (IHC). Terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) was used to detect apoptotic cells and determine the apoptosis index (AI). Analysis of variance (ANOVA) followed by Dunnett's test was used when more than two groups were compared. RESULTS: Saponin-treated animals showed increased SOD levels accompanied by decreased MDA, Scr and BUN levels (p < 0.05 vs. untreated controls); bcl-2 mRNA and protein levels were increased in transplanted kidney from treated animals, while bax mRNA and protein levels were decreased (p < 0.05 vs. untreated controls). AI was significantly decreased in transplanted kidneys from treated animals relative to untreated controls (p < 0.05 vs. untreated controls). CONCLUSION: This study clearly demonstrates the protective effects on IRI after kidney transplantation, which may be explained by decreased lipid peroxidation and inhibition of apoptosis.


Assuntos
Codonopsis , Rejeição de Enxerto/prevenção & controle , Transplante de Rim/efeitos adversos , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/uso terapêutico , Traumatismo por Reperfusão/prevenção & controle , Animais , Apoptose/efeitos dos fármacos , Modelos Animais de Doenças , Sequestradores de Radicais Livres/metabolismo , Rejeição de Enxerto/metabolismo , Rejeição de Enxerto/patologia , Imuno-Histoquímica , Marcação In Situ das Extremidades Cortadas , Masculino , Malondialdeído/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/biossíntese , Proteínas Proto-Oncogênicas c-bcl-2/genética , RNA Mensageiro/genética , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/metabolismo , Traumatismo por Reperfusão/patologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Superóxido Dismutase/metabolismo , Resultado do Tratamento , Proteína X Associada a bcl-2/biossíntese , Proteína X Associada a bcl-2/genética
20.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue ; 23(3): 150-3, 2011 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-21366943

RESUMO

OBJECTIVE: To evaluate the effect of 7.5% hypertonic saline (HS) and 6% hydroxyethyl starch (HES) 130/0.4 on early fluid resuscitation for severe sepsis. METHODS: Prospective randomized control trial was carried out in intensive care unit (ICU) of the Affiliated Hospital of Jianghan University. One hundred and thirty five patients with severe sepsis were randomly divided into three groups, each group consisted of 45 patients. Patients in HS+HES group received lactated Ringer solution following 4 ml/kg of 7.5% HS and 6% HES 130/0.4 500 ml, those in HES group received lactated Ringer solution following 6% HES 130/0.4 500 ml, and those in the lactated Ringer group (RL group) received lactated Ringer solution only.Mean arterial pressure (MAP), oxygenation index (PaO2/FiO2),arterial lactate (Lac),lactate clearance rate, acute physiology and chronic health evaluation II(APACHEII) score,fluid infusion volume, urine output as well as incidence of multiple organ dysfunction syndrome (MODS), and mortality were compared among three groups at 6 hours and 24 hours after ICU admission. RESULTS: At 6 hours after ICU admission,MAP[mm Hg (1 mm Hg=0.133 kPa): 68.7 ± 3.0], PaO2/FiO2(mm Hg: 262.2 ± 17.4), lactate clearance rate [(21 ± 4)%] in HS+HES group were significantly higher than those in HES group [MAP: 63.8 ± 3.5,PaO(2)/FiO(2): 252.0 ± 21.2, lactate clearance rate: (11 ± 2)%] and RL group [MAP: 62.6 ± 3.6, PaO2/FiO2:248.4 ± 17.0,lactate clearance rate: (9 ± 1)%, all P <0.01]. Arterial Lac in HS+HES group (mmol/L: 3.5 ± 0.7) was significantly lower than that in HES group (4.1 ± 0.7) and RL group (4.0 ± 0.7, both P <0.01). There was no significant difference in APACHE II score between HS+HES group (13.2 ± 1.9) and HES group (14.0 ± 1.6), and the APACHEII score in HS+HES group was significantly lower than that in RL group (15.2 ± 1.7, P <0.01). At 24 hours after ICU admission, PaO2/FiO2 (mm Hg: 303.3 ± 17.3) was significantly higher in HS+HES group than that in HES group (282.9 ± 21.1) and RL group (268.9 ± 15.2, both P <0.01). There was no significant difference in MAP, arterial Lac, lactate clearance rate and APACHEII score among three groups. At 6 hours and 24 hours after ICU admission, fluid infusion volume in HS+HES group (ml, 6 hours: 1 877.8 ± 215.2, 24 hours: 5 475.6 ± 208.8) was markedly less than that in HES group (6 hours: 2 505.6 ± 276.2, 24 hours: 6 383.3 ± 287.4) and RL group (6 hours: 3 496.7 ± 325.5,24 hours: 7 439.6 ± 229.6), yet urine output in HS+HES group (ml, 6 hours: 294.2 ± 36.9, 24 hours : 2 793.8 ± 37.1) was significantly higher than that in HES group (6 hours: 248.9 ± 25.3, 24 hours : 2 248.9 ± 25.3) and RL group (6 hours: 178.9 ± 14.8, 24 hours: 2 000.4 ± 147.0, all P <0.01). The incidence of MODS in HS+HES group (6.7%) was statistically lower than that in RL group (28.9%, P <0.05), while no obvious difference was found between HS+HES group and HES group (17.8%, P >0.05). There was no significant difference in mortality among three groups (HS+HES group: 2.2%, HES group: 4.4%, RL group: 8.9%, all P >0.05). CONCLUSION: 7.5%HS and 6%HES 130/0.4 could improve the effect of early fluid resuscitation on severe sepsis, and it could lower the incidence of MODS.


Assuntos
Derivados de Hidroxietil Amido/uso terapêutico , Solução Salina Hipertônica/uso terapêutico , Sepse/terapia , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Choque Hemorrágico
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...