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1.
Chemosphere ; 239: 124759, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31518920

RESUMO

Ammonia is an important environmental stress factor in aquaculture. Long-term ammonia stress could affect the normal growth, and also increase the risk for the occurrence of various diseases. In order to learn the mechanism that ammonia caused the outbreak of the shrimp disease, transcriptomics and metabolomics approaches were used to analyze the differential expressions of the genes in hemocytes and different metabolites in the serum of the Pacific white shrimp Litopenaeus vannamei under ammonia exposure. Transcriptional analysis showed that 17 cell apoptosis related genes, seven phagocytosis related genes, 10 immunity related genes and seven cell cycle and lipid metabolism related genes showed differential expressions after ammonia exposure. Metabolomics analysis on the serum showed that 25 differential metabolites were identified in positive and negative ion patterns. They are involved in purine metabolism, amino acids metabolism and lipid metabolism. Injection of two up-regulated metabolites triethanolamine and oxypurinol to normal shrimp could induce apoptosis in normal shrimp. The total hemocytes counts in shrimp showed a significant decrease and the apoptotic cell ratio increased significantly under ammonia exposure. These results suggested that ammonia exposure increased the apoptosis of hemocytes, which affected the immunity of shrimp, and thus caused susceptibility to pathogenic infection. These data will help us understand the mechanism of ammonia stress leading to the immunity decline of shrimp.

2.
Bioresour Technol ; 295: 122253, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31630000

RESUMO

Microbial lipid production from lignocellulosic biomass has attracted much attention recently. In this study, T. dermatis 32903 was selected from eleven promising oleaginous yeast strains. Carbon to nitrogen ratio (C/N) was investigated and optimized to maximize lipid production. Dilute acid (DA) pretreated corn stover (CS) and dilute alkali (AL) pretreated CS were then used for microbial lipid production, resulting in lipid concentrations of 7.46 g/L and 6.81 g/L, with sugar to lipid yields reached 0.104 g/g and 0.101 g/g, respectively. Washing of DA-CS and AL-CS enhanced lipid production to 11.43 g/L and 20.36 g/L with sugar to lipid yields improved to 0.156 g/g and 0.186 g/g, respectively. As degradation products in pretreated biomass showed severe inhibition on lipid fermentation, eight typical degradation products were further investigated for their effects on lipid fermentation. T. dermatis 32903 exhibited high tolerance to furan derivatives and week acids, but lower tolerance to phenolic compounds.


Assuntos
Trichosporon , Zea mays , Álcalis , Biomassa , Fermentação , Hidrólise , Lipídeos
3.
Protein Cell ; 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31686352

RESUMO

In the original publication the Fig. 2 and the Supplementary Material 1 was incorrect. The correct version of Fig. 2 and the Supplementary Material are provided in this correction article. NESTIN should be corrected to PAX6 in Fig. 2C legend and at page 528 and Supplementary Material 1. NANOG should be corrected to PAX6 in Fig. 2C picture. Fig. 2. Differentiation and identification of NSCs from PCOS-derived iPSCs. (A) Schematic procedure of NSCs differentiation from iPSCs. NSC: Neural stem cell; EB: embryoid body. (B) The phenotype of specific differentiated NSCs. Scale bars = 100 µm. (C) Immunofluorescence images of the NSC markers SOX2 and PAX6. Scale bars = 50 µm. ZOOM, scale bars = 25 µm. (D) The mitochondrial respiration function of PCOS- and non-PCOS-derived iPSCs and NSCs. (E) Quantitative analysis of basal oxygen consumption, ATP production, maximal respiration, and proton leak. (F) Proposed neuroendocrine state in normal and PCOS patients. In normal patients, the GnRH pulsatile frequency is critical for steroidogenesis and follicular development. Low frequency pulses prefer FSH, and high frequency pulses favour LH. In PCOS, the increased GnRH release led to a high level of LH pulsatility, impairing the preferential release of FSH and follicular maturation, thus leading to polycystic ovaries. Red: increased; Blue: decreased. Solid arrow: up regulated; Dotted arrow: down regulated.

4.
J Am Heart Assoc ; 8(22): e012885, 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31718447

RESUMO

Background Inflammation is recognized as an important contributor of ischemia/reperfusion (I/R) damage after ischemic stroke. Sphingomyelin synthase 2 (SMS2), the key enzyme for the biosynthesis of sphingomyelin, can function as a critical mediator of inflammation. In the present study, we investigated the role of SMS2 in a mouse model of cerebral I/R. Methods and Results Cerebral I/R was induced by 60-minute transient middle cerebral artery occlusion in SMS2 knockout (SMS2-/-) mice and wild-type mice. Brain injury was determined by neurological deficits and infarct volume at 24 and 72 hours after transient middle cerebral artery occlusion. Microglia activation and inflammatory factors were detected by immunofluorescence staining, flow cytometry, western blot, and RT-PCR. SMS2 deficiency significantly improved neurological function and minimized infarct volume at 72 hours after transient middle cerebral artery occlusion. The neuroprotective effects of SMS2 deficiency were associated with (1) suppression of microglia activation through Toll-like receptor 4/nuclear factor kappa-light-chain-enhancer of activated B cells pathway and (2) downregulation of the level of galactin-3 and other proinflammatory cytokines. The mechanisms underlying the beneficial effects of SMS2 deficiency may include altering sphingomyelin components in lipid raft fractions, thus impairing the recruitment of Toll-like receptor 4 to lipid rafts and subsequently reducing Toll-like receptor 4/myeloid differentiation factor 2 complex formation on the surface of microglia. Conclusions SMS2 deficiency ameliorated inflammatory injury after cerebral I/R in mice, and SMS2 may be a key modulator of Toll-like receptor 4/nuclear factor kappa-light-chain-enhancer of activated B cells activation by disturbing the membrane component homeostasis during cerebral I/R.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31724249

RESUMO

In order to investigate the mechanism of genistein (Gen) in the treatment of climacteric syndrome, an in vivo study was performed to investigate the beneficial effects of genistein on the expression of P450 aromatase (P450 arom) and follicle-stimulating hormone receptor (FSHR) in the mouse ovary and uterus. Fifty female ICR mice (45 ± 5g, n = 50), aged 12 months, were divided into the following five groups with 10 animals in each: blank control group (CG), low-dose genistein group (L-Gen), middle-dose genistein group (M-Gen) and high-dose genistein group (H-Gen) (received 15, 30 and 60 mg/kg of genistein, respectively), and oestrogen group (EG; received 0.5 mg/kg diethylstilbestrol). The expression levels of the FSHR protein were determined by an immunohistochemical staining method. The expression of P450 arom, Cytochrome P450 19 (CYP19) and FSHR was quantified by real-time PCR. Immunohistochemical results showed that the expression levels of the FSHR protein in the M-Gen (average stained area: 20.79) and the H-Gen (average stained area: 21.21) groups were significantly stronger than in the CG (average area was 17.24) group (p < .05). The expression levels of CYP19 mRNA and P450 arom were positively correlated with the dose of genistein. Specifically, the relative expression levels in the H-Gen and EG groups were more than 1.5 times higher than in the CG group (p < .05). Genistein played a significant role in regulating aromatase and FSHR gene expression to improve perimenopausal ovarian and uterine function.

6.
Plant Physiol ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31694901

RESUMO

MicroRNAs(miRNAs) are small non-coding RNAs of ~21 nt in length, which have regulatory roles in many biological processes. In animals, proper functioning of the circadian clock, which is closely linked to the fitness of almost all living organisms, is regulated by miRNAs. However, to date, there have been no reports of the roles of miRNA in regulation of the plant circadian rhythm. Here, we report a natural variant of miR397 that lengthens the circadian period and controls flowering time in Arabidopsis (Arabidopsis thaliana). Highly conserved among angiosperms, the miRNA miR397 has two members in Arabidopsis: miR397a and miR397b. However, only miR397b significantly delayed flowering. Our results suggest that miR397b controls flowering by targeting CASEIN KINASE II SUBUNIT BETA3 (CKB3), in turn modulating the circadian period of CIRCADIAN CLOCK ASSOCIATED1 (CCA1). We further demonstrated that CCA1 directly bound to the promoter of MIR397B and suppressed its expression, forming a miR397b-CKB3-CCA1 circadian regulation feedback circuit. Evolutionary analysis revealed that miR397b is a newly evolved genetic variant in Arabidopsis, and the miR397b targeting mode may have a role in enhancing plant fitness. Our results provide evidence for miRNA-mediated circadian regulation in plants and suggest the existence of a feedback loop to manipulate plant flowering through the regulation of circadian rhythm.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31696302

RESUMO

INTRODUCTION: Next-generation sequencing (NGS)-based assays to understand various mutations and co-occurrence of genomic alterations in non-small cell lung cancer (NSCLC) have enabled understanding of treatment impact on clinical outcomes. METHODS: This retrospective study was conducted in 1353 formalin-fixed paraffin-embedded (FFPE) tissues from surgically resected, pre-TKI-treated NSCLC patients with identified gene alterations. Genomic DNA and RNA extraction was followed by NGS library preparation and sequencing using the Ion Ampliseq Colon and Lung Cancer Gene Panel V2 and the AmpliSeq RNA Lung Cancer Research Fusion Panel. RESULTS: A total of 2328 alterations in 25 genes were detected from the 1293 patients. DNA mutations and RNA fusions co-occurred in 27 patients with TP53 being the most common co-occurring DNA mutation (43.8%) with concurrent ALK fusions. Analysis of the 975 patients with EGFR mutations revealed that the incidence of dual EGFR L858R/T790M mutations was higher compared to EGFR 19del/T790M, and the mean allele fraction (MAF) of T790M was lower compared to 19del in dual EGFR 19del/T790M patients. CONCLUSION: NSCLC patients represented genetically heterogeneous subgroup with a high frequency of co-occurring mutations in cancer-associated pathways. This diverse mutational profile may have key clinical and research implications for understanding the variability of treatment outcome in pre-TKI-treated NSCLC population. The differences in the MAF of EGFR T790M may determine different responses to TKI therapy in patients harboring dual mutations.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31697226

RESUMO

A novel Vogesella strain, YM-1T, was recovered from human urine in PR China in 2017. Cells of strain YM-1T were Gram-stain-negative, rod-shaped, aerobic, motile, non-spore-forming and poly-ß-hydroxybutyrate-accumulating. The strain contained C16:1ω6c/C 16:1ω7c, C16:0 and C18:0ω7c as major fatty acids; phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified phospholipid as major polar lipids; and ubiquinone-8 as the predominant respiratory quinone. Comparison of 16S rRNA gene sequences indicated that this strain had highest similarities to Vogesella perlucida DS-28T (98.8 %) and Vogesella mureinivorans 389T (98.1 %). The results of phylogenetic analysis based on the 16S rRNA gene sequences revealed that the novel strain was clustered and well separated with V. perlucida DS-28T and V. mureinivorans 389T within the genus Vogesella. The average nucleotide identity (ANI) and amino acid identity (AAI) analyses showed that this strain was not identified as V. perlucida DS-28T or V. mureinivorans 389T, with values well below the threshold limit for species demarcation (ANI <88.1 %, AAI <88.6 %). Based on the above results, strain YM-1T is proposed to be a novel species of the genus Vogesella with the name Vogesella urethralis sp. nov. (YM-1T=NBRC 113779=CGMCC 1.17135).

9.
Artigo em Inglês | MEDLINE | ID: mdl-31692185

RESUMO

Cage-type compounds offer molecule-level confined spaces with different sizes, shapes, and functional groups for various molecule guests to achieve some specific behaviors/functions, such as ultrahigh affinity of host-guest recognition, multi-tunable photophysical property, and highly-stereoselective/photo-driven catalysis. Herein, we report the synthesis and characterization of three-dimensional tetraphenylethene-based octacationic cage with fluorescent property and host-guest recognition via CH•••π, π-π, and/or electrostatic interactions for polycyclic aromatic hydrocarbons (e.g. coronene) in organic media and water-soluble dyes (e.g. sulforhodamine 101) in aqueous media, respectively. The X-ray structure of cage⸧coronene exhibits a cuboid internal cavity with the size of approximate 17.2 Å (length) × 11.0 Å (width) × 6.96 Å (height) and "hamburger"-type host-guest complexes, which are hierarchically stacked into 1D nanotube and 3D supramolecular framework. Compared to its host-guest complexes, free cage possesses a similar but empty cavity in the crystalline state, indicating that the stability and rigidity of cage itself are consistent both in the absence and presence of guest. Furthermore, as a single-molecular fluorescent platform, tetraphenylethene-based octacationic cage with dye guests (e.g. sulforhodamine 101) can form host-guest complex with higher absolute quantum yield ( Φ F = 28.5%), larger excitation-emission gap (Δλ ex-em = 211 nm), longer lifetime (τ = 7.0 ns), compared to guest ( Φ F = 10.5%; Δλ ex-em = 11 nm; τ = 4.9 ns); and purer emission with a narrow full width at half-maxima (Δλ FWHM = 38 nm), compared to host (Δλ FWHM = 111 nm), respectively. This study provides insight into construct cationic cage with both fluorescence and host-guest properties, and might facilitate further design of multifunctional cages and other fluorescent supramolecular systems based on host-guest recognition.

10.
Artigo em Inglês | MEDLINE | ID: mdl-31701580

RESUMO

In this study, we investigated the effects of Saccharomyces cerevisiae (SC), Bacillus subtilis (BS) and Enterococcus faecalis (EF), singly and in combination, on the dry matter intake (DMI), milk production and composition, and faecal microflora of Saanen dairy goats. Fifty goats were randomly divided into five groups: (a) basal diet (control); (b) basal diet + SC; (c) basal diet + BS; (d) basal diet + EF; and (e) basal diet + mixed probiotics. Each treated animal received 5 g/d of probiotics for a total administration of 5 × 1,011 CFU/goat per day. The inclusion of B. subtilis and E. faecalis in the diet of lactating Saanen goats increased DMI (p < .05). Enhanced milk yield was observed with BS and EF. Milk fat percentage was significantly increased by feeding mixed probiotics compared with the control (p < .05); supplying SC, BS and mixed probiotics enhanced the protein percentage (p < .05). The milk lactose percentage in the SC and BS groups was higher than in the control (p < .05). The amount of milk total solids was higher after feeding EF or mixed probiotics than in the control group (p < .05). Non-fat solids showed no notable differences among groups (p > .05). There was no significant influence on gut bacterial abundance and diversity from adding these three probiotics, singly or in combination. Bacteroidales, Escherichia-Shigella and Christensenellaceae abundances were decreased by supplying these probiotics but Succinivibrionaceae increased. In conclusion, there were positive influences of probiotic feed supplementation on intake, milk performance and intestinal microecology.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31702495

RESUMO

BACKGROUND: Previous clinical studies have suggested that apolipoprotein M (apoM) is involved in glucose metabolism and play a causative role in insulin sensitivity. OBJECTIVE: We aimed to explore the potential mechanism of apoM on modulating glucose homeostasis and analyze differentially expressed genes by employing ApoM deficient (ApoM-/-) and wild type (WT) mice. METHOD: The metabolism of glucose in the hepatic tissues of high-fat diet ApoM-/- and WT mice was measured by a glycomics approach. Bioinformatic analysis was applied for analyzing the levels of differentially expressed mRNAs in the liver tissues of these mice. The insulin sensitivity of ApoM-/- and WT mice was compared using insulin tolerance test and the phosphorylation levels of protein kinase Akt (AKT) following insulin stimulation in different tissues were examined by Western blot. RESULTS: The majority of the hepatic glucose metabolites exhibited lower concentration levels in the ApoM-/- mice compared with those of the WT mice. Gene Ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that ApoM deficiency affected the genes associated with the metabolism of glucose. The insulin tolerance test suggested that insulin sensitivity was impaired in ApoM-/- mice. The phosphorylation levels of AKT in muscle and adipose tissues of ApoM-/- mice were significantly diminished in response to insulin stimulation compared with those noted in WT mice. CONCLUSION: ApoM deficiency led to the disorders of glucose metabolism and altered glucose metabolism related genes in mice liver. In vivo data indicated that apoM might augment insulin sensitivity by AKT-dependent mechanism.

13.
Spine J ; 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31734451

RESUMO

BACKGROUND CONTEXT: Radiographic realignment objectives for the surgical correction of adult spinal deformity (ASD) has been well-described. However, the optimal sagittal spinopelvic alignment after corrective osteotomy for thoracolumbar kyphosis secondary to ankylosing spondylitis (AS) is still unknown so far. PURPOSE: To comprehensively investigate the relationship between radiographic parameters and clinical outcomes measured by Oswestry Disability Index (ODI) and Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) in thoracolumbar kyphosis patients caused by AS following one-level pedicle subtraction osteotomy (PSO) and to determine the optimal sagittal alignment at a minimum of two-year follow-up. STUDY DESIGN: A retrospective review of consecutive AS patients from one institution (2006-2016). PATIENT SAMPLE: One hundred AS-related thoracolumbar kyphosis patients who underwent one-level PSO with a minimum of 2-year follow-up (range, 2-10 years). OUTCOME MEASURES: Spinopelvic parameters at final follow-up (≥2 years) included thoracic kyphosis (TK), lumbar lordosis (LL), osteotomized vertebra angle (OVA), pelvic tilt (PT), sacral slope (SS), pelvic incidence (PI), sagittal vertical axis (SVA), spinosacral angle (SSA), T1 pelvic angle (TPA), spinopelvic angle (SPA), and global kyphosis (GK). Clinical outcomes at final follow-up (≥2 years) was evaluated by ODI and BASDAI, and ODI<20 was defined as good clinical outcome. METHODS: Pre- and postoperative radiographic and clinical outcomes were compared by paired samples t-test. The correlation between the postoperative radiographic parameters and clinical outcomes was investigated by Pearson correlation analysis. Linear regression analysis was performed to construct predictive models of the clinically relevant radiographic parameters based on ODI scores and to determine the realignment goals. Multiple stepwise regression analysis was applied to figure out the major radiographic contributors of ODI score at the final follow-up. RESULTS: In total, one hundred AS patients (92 males and 8 females) with a mean age of 34.7±9.5 years (range, 17-63 years) were followed up for 38.6±17.5 months (range, 24-120 months). At the final follow-up, PT and TPA were positively correlated with both ODI and BASDAI score (P<0.05). While SS, SSA, and SPA were negatively associated with the score of ODI (P<0.05), and BASDAI was negatively related to SPA (P<0.05). Predicting regression models of the clinically-relevant radiographic parameters were built based on the ODI score at the last follow-up. CONCLUSIONS: Based on the regression models, the optimal sagittal alignment of AS patients satisfying good clinical outcome (ODI<20) at a minimum of two-year follow-up was: PT<24°, SSA>108°, TPA<22°, and SPA>152°. Realizing the aforementioned realignment goals may contribute to satisfied clinical outcome for AS patients with thoracolumbar kyphosis undergoing one-level PSO.

14.
J Clin Pharm Ther ; 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31756280

RESUMO

WHAT IS KNOWN AND OBJECTIVE: Clinical use of fluconazole against fungal infections in renal transplant patients is complicated by the potentially marked and unpredictable drug-drug interactions (DDIs). We report a case of tacrolimus-fluconazole DDI in a stable renal transplant recipient and describe the mechanism, magnitude and duration of this DDI through a literature review. CASE SUMMARY: A 38-year-old woman experienced a 9.1-fold increase in dose-normalized tacrolimus trough level (trough concentration/weight-normalized daily dose) and an 87% decrease in weight-normalized daily dose (daily dose/body weight) in the treatment of documented Candida albicans oesophagitis by fluconazole. After discontinuation of fluconazole for 161 day, a 26% reduction in weight-normalized daily dose was required to maintain therapeutic exposure. WHAT IS NEW AND CONCLUSION: Oral fluconazole has a more significant impact on its drug interactions with tacrolimus than intravenous fluconazole. Gene screening for CYP3A5 6986 A>G and ABCB1 3435 C>T in organ transplant recipients may help in preventing DDI and facilitating tacrolimus dose adjustment.

15.
Sci Total Environ ; 694: 133625, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31756838

RESUMO

Thallium (Tl) contamination caused by the industrial wastewater leakage has become a serious environmental problem due to thallium's high toxicity. In this study, a novel titanium­iron magnetic nano-sized adsorbent was synthesized and applied for the effective removal of thallium(I). The physicochemical properties of the adsorbent were investigated by a series of techniques such as scanning electron microscope (SEM), X-ray powder diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). About 83% of equilibrium adsorption capacity could be accomplished within the initial 30 min. The adsorption of Tl(I) was found to be highly dependent on solution pH. The maximum adsorption capacity of Tl(I) was 111.3 mg/g at pH 7.0. The presence of such co-existing cations as Na+, Mg2+, Ca2+ and Cu2+ could have a certain influence on the uptake of Tl(I). The adsorption mechanism was proposed as a surface complexation process of Tl(I) ions by binding to deprotonated sites of hydroxyl groups on the adsorbent surface. The prepared magnetic adsorbent would be suitable for effectively treating thallium-containing water due to its promising adsorption ability towards Tl(I) and ease in operation.

16.
Int J Biol Macromol ; 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31759027

RESUMO

This study aimed to investigate the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ). Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA). Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes. Spearman's correlation network analysis revealed that key microbial phylotypes responding to GFP intervention were strongly correlated with the glucose and lipid metabolic disorders associated parameters. Moreover, GFP treatment regulated mRNA expression levels of the genes responsible for hepatic glucose and lipid metabolism. Itisnoteworthythat GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver. These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.

17.
J Cell Mol Med ; 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31709761

RESUMO

Emerging studies have shown that long noncoding RNA (lncRNA) TUG1 (taurine-up-regulated gene 1) plays critical roles in multiple biological processes. However, the expression and function of lncRNA TUG1 in cerebral ischaemia/reperfusion injury have not been reported yet. In this study, we found that LncRNA TUG1 expression was significantly up-regulated in cultured MA-C cells exposed to OGD/R injury, while similar results were also observed in MCAO model. Mechanistically, knockdown of TUG1 decreased lactate dehydrogenase levels and the ratio of apoptotic cells and promoted cell survival in vitro. Moreover, knockdown of TUG1 decreased AQP4 (encoding aquaporin 4) expression to attenuate OGD/R injury. TUG1 could interact directly with miR-145, and down-regulation of miR-145 could efficiently reverse the function of TUG1 siRNA on AQP4 expression. Finally, the TUG1 shRNA reduced the infarction area and cell apoptosis in I/R mouse brains in vivo. In summary, our results suggested that lncRNA TUG1 may function as a competing endogenous RNA (ceRNA) for miR-145 to induce cell damage, possibly providing a new therapeutic target in cerebral ischaemia/reperfusion injury.

18.
Artigo em Inglês | MEDLINE | ID: mdl-31745779

RESUMO

Thallium as a highly toxic metal element has been listed as one of priority drinking water contaminants. In this study, manganese oxide nanoparticles were synthesized through a simple hydrothermal method and applied for the removal of thallium(I). The adsorbent was composed of numerous needle-like nanorods and had an average volume diameter of 230 nm after heat-drying procedure. The crystal form of adsorbent was determined as α-MnO2. The adsorbent exhibited a much faster adsorption rate than most of previously reported adsorbent, achieving over 66.4% of equilibrium adsorption capacity in the first 10 min. The adsorption process was found to be highly affected by solution pH and higher than 100 mg/g of adsorption capacity could be obtained in a wide pH range of 6.0-10.0. The isotherm study indicated that the adsorption of Tl(I) on the adsorbent was favorable and governed by a chemisorption process, with the maximum adsorption capacity of 505.5 mg/g at pH 7.0. The adsorption process was confirmed to be thermodynamically spontaneous and endothermic. The presence of Na+, K+, Mg2+, Ca2+, and Cu2+ cations had certain negative effects on the uptake of Tl(I). Based on the batch experiments and XPS analysis, the deprotonated hydroxyl groups that bonded to manganese atoms worked as the binding sites for the effective removal of Tl(I) ions and no redox reaction occurred during the adsorption process.

19.
Mol Med Rep ; 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31746358

RESUMO

It has previously been demonstrated that hyperglycemia­induced oxidative stress and inflammation are closely associated with the development of diabetic complications, including diabetic neuropathy. Additionally, mitochondrial ATP­sensitive potassium (Mito­K­ATP) channels play a homeostatic role on blood glucose regulation in organisms. Molecular hydrogen (H2) exhibits anti­inflammatory, anti­antioxidative and anti­apoptotic properties and can be used to treat more than 71 diseases safely. In addition, the diabetes animal models which are set up using streptozotocin (STZ) injection, is a type of high long­term stability, low animal mortality rate and security method. The aim of the current study was to assess the value of hydrogen­rich saline (HS) in diabetic peripheral neuropathy (DPN) treatment and to determine its associated mechanisms in STZ­induced diabetic experimental rats. Additionally, the effects of the Mito­K­ATP channels, oxidative stress, inflammatory cytokines and apoptosis on DPN were also evaluated. From week 5 of STZ injections, HS (2.5, 5 and 10 ml/kg) was injected into the rat abdominal cavity every day for a period of 4 weeks. The results of the current study demonstrated that HS significantly reduced behavioral, biochemical and molecular effects caused by DPN. However, 5­hydroxydecanoate, a selective Mito­K­ATP channels general pathway inhibitor, partially eliminated the therapeutic effect of HS on DPN. These results indicated that the use of HS may be a novel strategy to treat DPN by activating the Mito­K­ATP pathway and reducing oxidative stress, inflammatory cytokines and apoptosis.

20.
Org Lett ; 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31742419

RESUMO

An electrophilic thiocyano semipinacol rearrangement of allylic alcohols has been achieved for the first time by using N-thiocyano-dibenzenesulfonimide (NTSI). This approach provides a direct, simple, and efficient strategy for the formation of thiocyano carbonyl compounds with moderate to excellent yields. Meanwhile, an all-carbon quaternary center was rapidly constructed. In addition, an asymmetric version of this tandem reaction was preliminarily investigated.

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