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1.
Carbohydr Polym ; 224: 115172, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31472830

RESUMO

Chitosan/calcium phosphate nanosheet as a promising antigen carrier was prepared via the direct mixture of modified chitosan, PBS and CaCl2. Specifically, chitosan was modified with catechol groups, and its water solubility under neutral conditions was improved. Then, nanosheet was formed by mixing modified chitosan and PBS followed by addition of CaCl2. Antigen was entrapped into the nanosheet via coprecipitation during its preparation. The nanosheet composed of CaHPO4·2H2O crystals, was internalized by dendritic cells (DCs) via macropinocytosis with enhanced efficiency. The antigen endosomal escape induce by nanosheet was successful observed with intracellular Lysotracker and Lamp-1 staining. Moreover, DCs activation was triggered by the nanosheet along with the up-regulated expression of co-stimulation marker and production of Th1-type cytokines. More importantly, cross-presentation of antigens achieved by the nanosheet was markedly increased when compared to free antigen. Therefore, chitosan/calcium phosphate nanosheet could be used as a vaccine carrier for effective cross-presentation of exogenous antigens.

2.
Nanotechnology ; 30(46): 465301, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31476139

RESUMO

To design a rotor with recoverable deformation for conversion between rotation and translation in a nanodevice, an internally hydrogenated deformable part (HDP) was introduced in the carbon nanotube-based rotor. Initially, under van der Waals (vdW) force, the hydrogenated areas on the HDP curved toward the rotating axis. When a rotational frequency was exerted on the rotor, the hydrogenated parts on the HDP were separated under strong centrifugal force. Translational motion of the free edge of the rotor was generated synchronously during deformation of the HDP. Once removing the input rotation, the rotor would stop rotating by friction from the stators, and the HDP shrank back by strong vdW force but weakening centrifugal force. Hence, the nanoconvertor has recoverability, which was verified by molecular dynamics simulations with considering the effects of hydrogenation schemes and input rotational frequency at room temperature. Conclusions were drawn for a design of a nanodevice based on the present rotation-translation nanoconvertor model.

3.
PLoS One ; 14(9): e0221729, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31483808

RESUMO

Past research on the process of extinguishing a fire typically used a traditional linear water jet falling point model and the results ignored external factors, such as environmental conditions and the status of the fire engine, even though the water jet falling point location prediction was often associated with these parameters and showed a nonlinear relationship. This paper constructed a BP (Back Propagation) neural network model. The fire gun nozzle characteristics were included as model inputs, and the water discharge point coordinates were the model outputs; thus, the model could precisely predict the water discharge point with small error and high precision to determine an accurate firing position and allow for the timely adjustment of the spray gun. To improve the slow convergence and local optimality problems of the BP neural network (BPNN), this paper further used a genetic algorithm to optimize the BPNN (GA-BPNN). The BPNN can be used to optimize the weights in the network to train them for global optimization. A genetic algorithm was introduced into the neural network approach, and the water jet landing prediction model was further improved. The simulation results showed that the prediction accuracy of the GA-BP model was better than that of the BPNN alone. The established model can accurately predict the location of the water jet, making the prediction results more useful for firefighters.

4.
Cancer Res ; 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31484669

RESUMO

The genetic events occuring in recurrent nasopharyngeal carcinoma (rNPC) are poorly understood. Here, we performed whole-genome and whole-exome sequencing in 55 rNPC and 44 primarily diagnosed NPC (pNPC) patients; with 7 patients having paired rNPC and pNPC samples. Previously published pNPC exome data were integrated for analysis. rNPC and pNPC tissues had similar mutational burdens, however, the number of clonal mutations was increased in rNPC samples. TP53 and three NF-κB pathway components (TRAF3, CYLD and NFKBIA) were significantly mutated in both pNPC and rNPC. Notably, mutations in TRAF3, CYLD and NFKBIA were all clonal in rNPC, however, 55.6-57.9% of them were clonal in pNPC. In general, the number of clonal mutations in NF-κB pathway-associated genes was significantly higher in rNPC than in pNPC. The NF-κB mutational clonality was selected and/or enriched during NPC recurrence. The amount of NF-κB translocated to the nucleus in samples with clonal NF-κB mutants was significantly higher than that in samples with subclonal NF-κB mutants. Moreover, the nuclear abundance of NF-κB protein was significantly greater in pNPC samples with local regional relapse than in those without relapse. Further, high nuclear NF-κB levels were an independent negative prognostic marker for locoregional relapse-free survival in pNPC. Lastly, inhibition of NF-κB enhanced both radiosensitivity and chemosensitivity in vitro and in vivo. In conclusion, NF-κB pathway activation by clonal mutations plays an important role in promoting the recurrence of NPC. Moreover, nuclear accumulation of NF-κB is a prominent biomarker for predicting locoregional relapse-free survival.

5.
Bioresour Technol ; 293: 122081, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31479855

RESUMO

It is very important to utilize associated vegetable products as resources, especially in large-scale vegetable cultivation areas. In this study, pepper straw, a vegetable waste, was pyrolyzed into pepper straw biochars (PBs) to investigate their sorption potential for phthalate acid esters (PAEs). The results showed that PBs have porous structures and abundant surface functional groups. Dibutyl phthalate (DBP) and dimethyl phthalate (DMP) removal by PBs was divided into two stages, fast and slow sorption. The PBs pyrolyzed at 500 °C showed greater DBP and DMP sorption capacity than those pyrolyzed at 400 and 600 °C. Both chemical and physical sorption occurred in the whole sorption process of PAEs to PBs. It is proposed that converting pepper straw into biochars to use as sorbents could be an environmentally friendly way of vegetable waste resource utilization.

6.
J Cell Physiol ; 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31489637

RESUMO

Platelet endothelial cell adhesion molecule-1 (PECAM-1) is a member of the immunoglobulin superfamily and is expressed by hematopoietic and endothelial cells (ECs). Recent studies have shown that PECAM-1 plays a crucial role in promoting the development of the EC inflammatory response in the context of disturbed flow. However, the mechanistic pathways that control PECAM-1 protein stability remain largely unclear. Here, we identified PECAM-1 as a novel substrate of the APC/Cdh1 E3 ubiquitin ligase. Specifically, lentivirus-mediated Cdh1 depletion stabilized PECAM-1 in ECs. Conversely, overexpression of Cdh1 destabilized PECAM-1. The proteasome inhibitor MG132 blocked Cdh1-mediated PECAM-1 degradation. In addition, Cdh1 promoted K48-linked polyubiquitination of PECAM-1 in a destruction box-dependent manner. Furthermore, we demonstrated that compared with pulsatile shear stress (PS), oscillatory shear stress decreased the expression of Cdh1 and the ubiquitination of PECAM-1, therefore stabilizing PECAM-1 to promote inflammation in ECs. Hence, our study revealed a novel mechanism by which fluid flow patterns regulate EC homeostasis via Cdh1-dependent ubiquitination and subsequent degradation of PECAM-1.

7.
Medicine (Baltimore) ; 98(36): e16981, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31490379

RESUMO

RATIONALE: Henoch-Schönlein purpura (HSP) is a common disease in children. However, HSP with intussusception and intestinal obstruction has a low morbidity in children and is occasionally seen in adults. Herein, a rare adult case of HSP complicated with intussusception and hemafecia that was successfully treated with surgery is described. PATIENT CONCERNS: A 19-year-old Chinese man suffered from HSP combined with intussusception and intestinal obstruction and presented with vomiting, diarrhea, abdominal pain, and rash. DIAGNOSIS: Henoch-Schönlein purpura with intussusception and intestinal obstruction. INTERVENTIONS: The patient underwent an emergency laparotomy and manual reset. OUTCOME: The patient was discharged 7 days after surgery in stable condition. We followed the patient to the sixth month after surgery. This patient has no long-term complications after surgery. LESSONS: HSP with intussusception and intestinal obstruction is rarely seen in adults. Patients with HSP often present with abdominal pain as the first symptom, which is easily confused with other diseases. Once the HSP is diagnosed, surgery should be performed as soon as possible, provided there is no absolute contraindication.

8.
Chemosphere ; 238: 124630, 2019 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-31473530

RESUMO

Mercury (Hg) mining activities are an important anthropogenic source of atmospheric Hg. The Xunyang Hg mine located in Shaanxi Province is the largest active Hg producing centre in China. To understand the biogeochemical processes of atmospheric Hg through Hg mining activities, six groups of experimental pots were carefully designed to investigate the effect of Hg mining activities on Hg contamination from atmospheric deposition in the local surface soils. Based on the variations of Hg in the soil from the experimental pots, the deposition flux and loading of Hg in the Xunyang Hg mining district were investigated. The results showed that the average concentration of total gaseous mercury (TGM) as high as 193 ±â€¯122 ng m-3 was observed in the ambient air, which was orders of magnitude higher than that in remote areas. The average deposition flux and annual loading of atmospheric Hg were 72 mg m-2 y-1 and 10 t y-1, respectively. The dominant atmospheric Hg deposition is within a distance range of 6.0-12 km from the Hg retorting facility, accounting for approximately 85% of the total Hg loading. After 14 months of exposure, total mercury (THg) concentrations in the soil from the experimental pots increased 0.35-9.5 times, and the highest concentrations of methylmercury (MeHg) (3.7 ±â€¯2.9 µg kg-1) in soil were observed in February. Concentrations as high as 643 µg kg-1 THg and 13 µg kg-1 MeHg in rice were observed in the second experimental year. Elevated concentrations of both THg and MeHg in rice indicated that the newly deposited atmospheric Hg was bioavailable, readily methylated, and taken up by rice, suggesting that the ongoing Hg mining activities cause serious Hg contamination in the soil-rice ecosystem and posed a threat to local residents in the Xunyang Hg mining area.

9.
Stem Cell Res Ther ; 10(1): 281, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31481100

RESUMO

Stem cell therapy has been applied in many fields. Basic and clinical studies on stem cell therapy for acute kidney injury (AKI) have been conducted. Stem cells have been found to exert renal protection through a variety of mechanisms, such as regulating the immune system and secreting growth factors, cytokines, and extracellular vesicles (EVs). Among them, EVs are considered to be important mediators for stem cell protection because they contain various biological components, including microRNAs (miRNAs). miRNAs are a class of small RNAs that function in posttranscriptional gene regulation. A number of studies have confirmed that miRNAs in stem cell-derived EVs can protect from AKI. miRNAs can enter the injured renal tissue through EVs released from stem cells, thereby exerting anti-inflammatory, anti-apoptotic, anti-fibrotic, and pro-angiogenesis effects on AKI. However, the stem cell sources and AKI models used in these studies have differed. This article will summarize the miRNAs that play a role in kidney protection in stem cell EVs and clarifies the treatment characteristics and mechanisms of different miRNAs. This may provide a reference for clinical practice for acute and chronic kidney diseases.

10.
JAMA Netw Open ; 2(9): e199292, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31483464

RESUMO

Importance: Only a small fraction of patients with cancer receiving immune checkpoint therapy (ICT) respond, which is associated with tumor immune microenvironment (TIME) subtypes and tumor-infiltrating lymphocytes (TILs). Objective: To examine whether germline variants of natural killer (NK) cells, a key component of the immune system, are associated with TIME subtypes, the abundance of TILs, response to ICT, clinical outcomes, and cancer risk. Design, Setting, and Participants: This genetic association study explored TIME subtypes and examined the association of the germline genomic information of patients with cancer with TIME subtypes, abundance of TILs, response to ICT, prognosis, and cancer risk. Clinical information, tumor RNA sequencing, and whole-exome sequencing (WES) data of paired normal samples of patients with 13 common cancers (n = 5883) were obtained from the Cancer Genome Atlas. The WES data of individuals with no cancer (n = 4500) were obtained from the database of Genotypes and Phenotypes. Data collection and analysis took place in March 2017. Main Outcomes and Measures: Associations between the number of germline defective genes in NK cells and survival time and the abundance of TILs. Results: Based on tumor RNA sequencing data, tumors were stratified into TIME-rich, TIME-intermediate, and TIME-poor subtypes. Tumors of TIME-rich subtype had more TILs (TIL-NK cells in TIME-rich head and neck squamous cell carcinoma [HNSC] tumors: t = 4.85; 95% CI of the difference, 0.01-0.03; P = 2.19 × 10-6) compared with TIME-intermediate HNSC tumors (t = 3.70; 95% CI of the difference, 0.01-0.03; P < .001), better prognosis (patients with HNSC: hazard ratio, 0.65; 95% CI, 0.41-1.02; P = .054) compared with TIME-intermediate and TIME-poor subtypes, and better ICT response (patients with melanoma: odds ratio [OR], 4.45; 95% CI, 0.99-27.08; P = .04). Patients with TIME-rich tumors had significantly fewer inherited defective genes in NK cells than patients with TIME-intermediate and TIME-poor tumors (patients with HNSC: OR, 0.49; 95% CI, 0.26-1.07; P = .005). Similarly, patients with cancer had significantly more inherited defective genes in NK cells than individuals with no cancer (patients with HNSC: OR, 19.09; 95% CI, 4.30-315.96; P = 6.21 × 10-4). Among 11 of 13 common cancers, the number of heritable defective genes in NK cells was significantly negatively associated with survival (patients with HNSC: hazard ratio, 1.77; 95% CI, 1.18-2.66; P = .005), abundance of TILs (patients with HNSC: R = -0.25; 95% CI, -0.65-2.17; P = 0.02), and response to ICT (patients with melanoma: OR, 4.45; 95% CI, 0.99-27.08; P = .04). Conclusions and Relevance: These results suggest that individuals who have more inherited defective genes in NK cells had a higher risk of developing cancer and that these inherited defects were associated with TIME subtypes, recruitment of TILs, ICT response, and clinical outcomes. The findings have implications for identifying individuals at risk for developing cancer of many types based on germline variants of NK cells and for improving existing ICT and chimeric antigen receptor-T cell therapy by adoptive transfer of healthy NK cells to patients with TIME-intermediate and TIME-poor tumors.

11.
Opt Lett ; 44(17): 4139-4142, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31465348

RESUMO

We study terahertz (THz) optoelectronic properties of monolayer (ML) MoS2 placed on different substrates such as SiO2/Si, sapphire, and quartz. Through the measurements of THz Fourier transform spectroscopy (2.5-6.5 THz) and THz time-domain spectroscopy (TDS, 0.2-1.2 THz), we find that the real part of optical conductivity increases for ML MoS2 on SiO2/Si and sapphire substrates and decreases for it on quartz with increasing radiation frequency. It is shown that the complex optical conductivity for ML MoS2, obtained from THz TDS measurements, can fit very well to the Drude-Smith formula. Thus, the dependence of optical conductivity of ML MoS2 on different substrates can be understood via a mechanism of electronic localization, and the electron density, relaxation time, and localization factor of the sample can be determined optically. Furthermore, we examine the influence of temperature on these key parameters in ML MoS2 on different substrates. The results obtained from this Letter indicate that THz spectroscopy is a very powerful tool in studying and characterizing ML MoS2-based electronic systems, especially in examining the electronic localization effect which cannot be directly measured in conventional electrical transport experiment. This Letter is relevant to an in-depth understanding of the optoelectronic properties of ML MoS2 and of the proximity effect induced by different substrates.

13.
Gait Posture ; 73: 262-268, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31382233

RESUMO

BACKGROUND: Previous studies showed functional ankle instability (FAI) patients have morphological ligamentous abnormality, despite having no apparent joint laxity. RESEARCH QUESTION: Whether tibiotalar and subtalar joints hypermobility exists in FAI patients during stance phase of walking, remains controversial. METHODS: Ten unilateral FAI patients, ten unilateral lateral ankle sprain (LAS) copers and ten healthy controls were included. A dual fluoroscopy imaging system was utilized to capture the fluoroscopic images of tibiotalar and subtalar joint during the stance phase of walking. Kinematic data from six degrees of freedom were calculated utilizing a solid modeling software. The range of motion and joint excursions about six degrees of freedom were compared among the three groups. The correlations between range of motion and Cumberland Ankle Instability Tool (CAIT) scores were assessed utilizing the Spearman's correlation coefficient (r). RESULTS: During the stance phase, the FAI patients and LAS copers showed larger tibiotalar anterior/posterior translation than the healthy controls (FAI patients, p = .013; LAS copers, p = .002). The FAI patients also showed significantly larger lateral/medial translation (p = .035) and inversion/eversion rotation (p = .003) of subtalar joints than healthy controls. By contrast, the subtalar joints of the LAS copers were not different from those of the healthy controls in the lateral/medial translation (p = .459) and inversion/eversion rotation (p = .091). CAIT scores were negatively correlated with range of motion. SIGNIFICANCE: During the stance phase of walking, FAI patients showed significantly larger hypermobility of subtalar joints than healthy controls, contrary to the LAS copers. These findings justify the utilization of dual fluoroscopy imaging system to detect joint hypermobility in FAI patients. Treatment for FAI patients may require stabilization of the subtalar joint.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31382609

RESUMO

Background: Dementia is one of the public health priorities in China. This study aimed to examine the hospitalization costs of patients with dementia and analyzed the factors associated with their inpatient costs. Methods: This was a prevalence-based, observational study using claims data derived from two urban insurance schemes during the period from 2008 through 2013 in Guangzhou. The extended estimating equations model was performed to identify the main drivers of total inpatient costs. Results: We identified 5747 dementia patients with an average age of 77.4. The average length of stay (LOS) was 24.2 days. The average hospitalization costs per inpatient was Chinese Yuan (CNY) 9169.0 (CNY 9169.0 = US$1479.8 in 2013). The mean inpatient costs for dementia patients with the Urban Employee-based Basic Medical Insurance (UEBMI) scheme (CNY 9425.0 = US$1521.1) were higher than those for patients with the Urban Resident-based Basic Medical Insurance scheme (CNY 7420.5 = US$1197.6) (p < 0.001). Having UEBMI coverage, dementia subtypes, having hypertension, being admitted in larger hospitals, and longer LOS were significantly associated with hospitalization costs of dementia. Conclusions: The costs of hospitalization for dementia were high and differed by types of insurance schemes. Dementia was associated with substantial hospitalization costs, mainly driven by insurance type and long LOS. These findings provided economic evidence for evaluating the burden of dementia in China.

15.
Langmuir ; 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31424228

RESUMO

Mussel-inspired phenol/amine deposition in wet adhesion provides a cost-effective strategy to readily fabricate functional coatings for a wide range of applications. The adhesion of phenol/amine to different substrates and the cohesion between phenol/amine are believed to play critical roles during the deposition process. However, the understanding on the correlation between the deposition capability and interaction behavior involved in the coating formation still remains incomplete, which limits further developing phenol/amine-based functional materials and coatings. In this work, we correlated the interaction forces between two phenol/amine coatings and between phenol/amine coating and different substrate surfaces with the deposition capability of phenol/amine using surface forces apparatus and atomic force microscopy. The mass ratio of phenol and amine was found to significantly influence the deposition behavior through regulating the surface properties of phenol/amine aggregates' cohesion strength between phenol/amine coatings. Furthermore, the strong adhesion measured between phenol/amine coating and substrates with varying surface chemistry was demonstrated to be able to effectively initiate the surface-independent phenol/amine deposition as well as the continuous growth of phenol/amine coatings. This work provides useful insights into the fundamental understanding of the interactions and deposition mechanism during phenol/amine deposition process, with implications for developing advanced phenol/amine-based coating materials for a wider range of applications.

16.
Mol Ther ; 2019 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-31401147

RESUMO

MicroRNA is an endogenous, small RNA controlling multiple target genes and playing roles in various tumorigenesis processes. In this study, our results revealed that miR-602 expression levels in tumor tissues and preoperative serum from esophageal squamous cell carcinoma (ESCC) patients were higher than those in non-tumorous tissues and healthy volunteers. miR-602 overexpression was closely related to lymph node metastasis and TNM stages and correlated short overall, and it acted as an independent prognostic marker of ESCC. The methylation status of the miR-602 gene indicated more frequent hypomethylation of the CpG sites located upstream of the miR-602 gene in the ESCC tissues than in the adjacent normal tissues, and the methylation status of miR-602 correlated inversely with its expression levels. Subsequently, miR-602 overexpression promoted ESCC proliferation and metastasis and regulated cell cycles in vitro and in vivo. Mechanistically, a dual-luciferase experiment validated that Fork head box (FOX)K2 (FOXK2) was a direct target of miR-602. More importantly, systemic delivery of formulated miR-602 antagomir could reduce tumor growth and increased FOXK2 protein expression in nude mice. This work provides novel insight into the molecular pathogenesis of ESCC.

17.
Plant Biol (Stuttg) ; 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31419841

RESUMO

Verticillium wilt, an infection caused by the soilborne fungus Verticillium dahliae, is one of the most serious diseases in cotton. No effective control method against V. dahliae has been established, and the infection mechanism of V. dahliae in upland cotton remains unknown. GFP-tagged V. dahliae isolates with different pathogenic abilities were used to analyse the colonization and infection of V. dahliae in the roots and leaves of different upland cotton cultivars, the relationships among infection processes, the immune responses and the resistance ability of different cultivars against V. dahliae. Here, we report a new infection model of V. dahliae in upland cotton plants. V. dahliae can colonize and infect any organs of upland cotton plants and then spread to the entire plant from the infected organs through the surface and interior of the organs. In fact, vascular tissue was found to not be the sole transmission route of V. dahliae in cotton plants. In addition, the rate of infection of a V. dahliae isolate with strong pathogenicity was notably faster than that of an isolate with weak pathogenicity. The resistance of upland cotton to Verticillium wilt was related to the degree of the immune response induced in plants infected with V. dahliae. These results provide a theoretical basis for studying the mechanism underlying the interaction between V. dahliae and upland cotton.

18.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 33(8): 923-928, 2019 Aug 15.
Artigo em Chinês | MEDLINE | ID: mdl-31407547

RESUMO

Objective: To research the value of virtual reality (VR) technology in the preoperative planning of transtrochanteric curved varus osteotomy for avascular necrosis of the femoral head (ANFH) in adults. Methods: Between June 2018 and November 2018, 7 patients (11 hips) with ANFH, who were treated with transtrochanteric curved varus osteotomy, were enrolled in the study. There were 4 males (7 hips) and 3 females (4 hips) with an average age of 31.9 years (range, 14-46 years). Among them, 3 patients were unilateral ANFH and 4 patients were bilateral ANFH. There was 1 patient (1 hip) of traumatic ANFH, 2 patients (4 hips) of alcohol-induced ANFH, 2 patients (3 hips) of hormonal ANFH, and 2 patients (3 hips) of idiopathic ANFH. All hips were Association Research Circulation Osseous (ARCO) stage Ⅲ. There were 5 hips for Japanese Investigation Committee (JIC) type C1 and 6 hips for type C2. There were 5 hips for China-Japan Friendship Hospital (CJFH) type L1,1 for type L2, and 5 for type L3. The disease duration ranged from 5 to 12 months (mean, 8 months). Preoperative Harris score was 53.91±7.66. The neck-shaft angle ranged from 128 to 143° (mean, 133.9°). VR technology was adopted for the preoperative planning. CT data were imported into the software to construct the morphology of necrotic area, and the transrtrochanteric varus osteotomy was simulated. The varus angle was designed according to the integrity rate of femoral head. The planned varus angle was 6 to 16° (mean, 9.7°). The transtrochanteric curved varus osteotomy was performed according to the preoperative planning, and the varus angle and loading area were confirmed under fluoroscopy. If the planned varus angle was too small, it would continue to increase under the fluoroscopy until a satisfactory varus angle. Postoperative changes of the neck-shaft angle were calculated and compared with the preoperative planned varus angle (error). The hip function was assessed by using the Harris score. Results: All incisions healed by first intention. All patients were followed up 6-11 months with an average of 8 months. The X-ray film at 2 days after operation showed that the neck-shaft angle was 112-135° (mean, 123.4°). The difference of the neck-shaft angle between pre- and post-operation was 6-16° (mean, 11.0°). Among them, the difference of the neck-shaft angle was consistent with planned varus angle in 5 hips, while the error of the remaining 6 hips was 1-4°. There was 1 patient (1 hip) of osteotomy nonunion at 4 months after operation, 1 patient (1 hip) of proximal femur fracture at 2 months after operation. The rest 5 patients (9 hips) obtained union at the osteotomy. At last follow-up, the Harris score was 82.18±16.35, showing significant difference when compared with preoperative score ( t=-5.195, P=0.000). Conclusion: VR technology is a brand-new preoperative planning method for transtrochanteric curved varus osteotomy in treating ANFH.

19.
Hum Vaccin Immunother ; : 1-9, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31403352

RESUMO

Enterovirus 71 (EV71) is one of the major causative agents for hand, foot and mouth disease (HFMD) in children. Although there are three inactivated virus-based HFMD vaccines licensed in China, alternative approaches have been taken to produce an effective and safer vaccine that is easier to manufacture in large scale. Among these, a virus-like particles (VLPs) based EV71 vaccine is under active development. For this purpose, an efficient methodology for the production of EV71-VLPs by recombinant technology is needed. We here report the construction and expression of the P1 and 3C genes of EV71 in Pichia pastoris for producing VLP-based EV71 vaccine antigen with a high yield and simple manufacturing process. Based on codon-optimized P1 and 3C genes, EV71-VLPs were efficiently expressed in Pichia pastoris system, and the expression level reached 270 mg/L. Biochemical and biophysical analyses showed that the produced EV71-VLPs consisted of processed VP0, VP1, and VP3 present as ~35nm spherical particles. The immune response as a function of EV71-VLPs and adjuvant dose ratio was investigated for vaccine development. Immunization with EV71-VLPs of 1-5 µg/dose and adjuvant of 225 µg/dose induced robust neutralizing antibody responses in mice and provided effective protection against lethal challenge in both maternally transferred antibody and passive transfer protection mouse models. Therefore, the yeast produced EV71-VLPs antigen is a promising candidate for the development of a vaccine against HFMD.

20.
Yonsei Med J ; 60(9): 832-841, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31433581

RESUMO

PURPOSE: Epirubicin is one of the most effective drugs against osteosarcoma. miR-1301 is involved in the occurrence and development of osteosarcoma. Whether miR-1301 is responsible for the chemosensitivity of osteosarcoma cells to epirubicin remains largely unknown. MATERIALS AND METHODS: U2OS and SAOS-2 cells were treated with various concentrations of epirubicin. Flow cytometry was employed to evaluate cell apoptotic rate. Cell proliferation was measured by Cell Counting Kit-8 assay. Western blot and quantitative real-time polymerase chain reaction were utilized to detect the expressions of B-cell lymphoma-2 (Bcl-2), Bcl-2 assaciated X protein (Bax), cleaved-caspase-3, cleaved-poly (ADP-ribose) polymerases (PARP1), TP53-regulated inhibitor of apoptosis 1 (TRIAP1), and microRNA-1301 (miR-1301). The relationship between miR-1301 and TRIAP1 was determined by luciferase reporter assay. RESULTS: Epirubicin inhibited proliferation in a dose-dependent manner, induced apoptosis, decreased the expression of Bcl-2, and increased the expressions of Bax, cleaved-caspase-3, and cleaved-PARP1 in osteosarcoma cells. miR-1301 was downregulated in U2OS and SAOS-2 cells. Importantly, epirubicin significantly increased the levels of miR-1301. Overexpression of miR-1301 suppressed proliferation and promoted apoptosis. Interestingly, those effects were enhanced by epirubicin. In contrast, miR-1301 depletion attenuated the epirubicin-mediated anti-osteosarcoma effect. miR-1301 negatively regulated the expression of TRIAP1 in U2OS and SAOS-2 cells. Furthermore, epirubicin inhibited the mRNA and protein levels of TRIAP1 by upregulating miR-1301 levels. Epirubicin suppressed cell proliferation by downregulating TRIAP1. CONCLUSION: miR-1301 was implicated in the chemosensitivity of osteosarcoma to epirubicin by modulating TRIAP1.

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