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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 245: 118942, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32977105

RESUMO

In this study, we report a rapid statistical approach used in determining the caprolactam (CPL) content in curdlan packaging films, which is based on the spectral data observed in the near-infrared (NIR) and Mid-infrared (MIR) regions. At the first stage of the study, the CPL content was added into the curdlan films prepared by controlling the concentration, and then the effect of the CPL concentration on the measured mechanical properties of the produced films were evaluated. At the next stage, the NIR and MIR spectra of the curdlan films with different CPL concentrations were recorded by using the FT-NIR and FT-IR spectroscopy technique, and the spectral data to be used in the regression models in our quantitative analyses were carefully selected. It was observed that the curdlan film with 5% CPL exhibited the best mechanical properties. The obtained best correlation parameters which are used in evaluation of CPL content through the observed NIR and MIR spectral data are Rp = 0.9552, RMSEP = 1.2506 (NIR); Rp = 0.9092 and RMSEP = 1.9136 (MIR), respectively. These optimal values support the expectation that our statistical approach based on NIR and MIR data can provide a rapid, accurate and nondestructive way of determining CPL content in curdlan packaging films.

2.
Chemosphere ; 262: 127707, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32755691

RESUMO

The corrosion mechanisms of nanoscale zero-valent iron (nZVI) vary with different geochemical constituents, which affect the reductive dechlorination process of trichloroethylene (TCE). In this study, the effect of nZVI anaerobic corrosion on the reductive dechlorination of TCE with different groundwater geochemical constituents (Ca2+-SO42-, Ca2+-HCO3-, Na+-NO3-) was investigated. Microscopic characterization by X-ray diffraction (XRD) and transmission electron microscopy (TEM) combined with pH, oxidation-reduction potential (ORP) and dissolved Fe2+ in solutions to illustrate the corrosion mechanism of nZVI. In the four systems including ultrapure water (UPW), the reduction of TCE conformed to pseudo-first-order kinetics, the generation of Cl- accorded with zero-order kinetics, and multi-step reaction kinetics was used to fit the generation and degradation of chlorinated byproducts (Dichloroethylene, DCEs). Compared with UPW system, the dissolution corrosion of Ca2+-HCO3- and Ca2+-SO42- promoted the reductive dechlorination of TCE (kobs, TCE = 0.658 ± 0.010 & 0.245 ± 0.028 d-1 and kobs, Cl- = 41.682 ± 1.016 & 20.623 ± 1.923 µM⋅d-1 for Ca2+-HCO3- & Ca2+-SO42-, respectively) and the degradation of DCEs (0.444 ± 0.036 & 0.244 ± 0.040 µM⋅d-1 for Ca2+-HCO3- & Ca2+-SO42-, respectively); redox-active NO3- competed for electrons and passivated the surface of nZVI, which limited the reductive dechlorination of TCE (kobs, TCE = 0.111 ± 0.025 d-1 & kobs, Cl- = 14.943 ± 0.664 µM⋅d-1) and the degradation of DCEs (0.078 ± 0.018 µM⋅d-1), and the passivation layer promoted the adsorption of TCE. This study from the perspective of nZVI corrosion provides a theoretical basis for the long-term application of nZVI technology in the remediation of TCE-contaminated sites with different groundwater geochemical types.

3.
Ultrason Sonochem ; 71: 105375, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-33166916

RESUMO

Herein, we investigated experimentally the dynamics of three laser-induced, same-sized, symmetrically aligned, and synchronized bubbles. Three synchronized laser beams split from the same beam using a Diffractive Optical Element splitter were focused on water, and then we obtained three bubbles. Another nanosecond laser pulse was used to probe the bubbles to obtain shadowgraphs. The exact delay of the excited and detected light was controlled using a delay generator. The results revealed that the maximum volumes of bubbles in arrays decrease as the normalized distance falls, while the lifetimes and translation increase. It was explained by the interaction between the acoustic radiation of bubbles and the surrounding bubbles. The shrinkage of linear bubble arrays exists an anomaly. The center bubbles were stretched, to ellipsoid, stick, even fractured, by the peripheral bubbles. The closer they are, the more distinct is the above phenomenon. However, when the normalized distance was sufficiently small, instead of being stretched, the center bubbles were compressed to disk shape and thus shrank with the whole array. Finally, the dependence of the distance on the energy transfer of the bubble system is also discussed.

4.
Clin Lung Cancer ; 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33160898

RESUMO

BACKGROUND: To compare the surgical outcomes of patients with clinical stage I ground-glass opacity (GGO) lung adenocarcinomas with maximum diameters of ≤ 2 cm who underwent lobectomy versus limited resection. PATIENTS AND METHODS: We retrospectively reviewed cases of clinical stage I GGO lung adenocarcinoma with a diameter ≤ 2 cm that were treated via lobectomy or limited resection in our department between January 2011 and September 2018. The clinical characteristics and surgical outcomes were analyzed using a propensity score-matched comparison and a Cox regression model. RESULTS: A total of 552 patients were identified; 128 patients with pure GGO were excluded. Four hundred twenty-four patients met our criteria, including 242 (57.1%) who underwent lobectomy and 182 (42.9%) who underwent limited resection. No perioperative mortality occurred in either group. The overall 5-year survival rate of the entire cohort was 88%. Patients who underwent limited resection tended to have a shorter operation time, smaller blood loss volume, fewer removed nodes, and a shorter postoperative stay. However, the groups did not differ in terms of postoperative complications. Lobectomy and limited resection could lead to equivalent overall survival in patients with GGO-dominant tumor, while lobectomy showed better overall survival than limited resection in patients with solid-dominant tumor. CONCLUSION: Patients with small GGO lung adenocarcinoma had a favorable prognosis after surgery. The oncologic surgical procedures of lobectomy and limited resection yielded comparable outcomes in patients with clinical stage I GGO-dominant lung adenocarcinomas ≤ 2 cm, while lobectomy showed better survival than limited resection in patients with solid-dominant tumor.

5.
Chemosphere ; : 128948, 2020 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-33220979

RESUMO

In this study, to improve the mechanical and thermal properties of curdlan film, a curdlan/nanocellulose (NC) blended film was prepared and characterized for the first time. NC was successfully prepared from microcrystalline cellulose (MCC) with NaOH/urea treatment. The particle size of NC was observed to be 70-140 nm by cryo-electron microscope (cryo-EM). The blended film was prepared by adding the NC to curdlan solution. The tensile strength (TS) of the blended film reached the maximum value of 38.6 MPa, and the elongation at break (EB) was 40%. The DSC curve showed that the heat absorption peak of the film was 240 °C, indicating that the blended film has good temperature stability. Additionally, some other film properties were also improved, including gas barrier properties and transparency. Obvious morphological and molecular differences between the blended film and the pure curdlan film were discovered by SEM and FTIR analysis. Finally, the blended film was used for the preservation of chilled meat and extended the storage time of meat to 12 days. These results provided a theoretical basis for future application and development of biodegradable film.

6.
Chem Commun (Camb) ; 56(86): 13093-13096, 2020 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-33034609

RESUMO

A novel viologen-based multifunctional Eu-MOF was obtained by integrating a luminescent component Eu(NO3)3·6H2O and a viologen-functionalized ligand. The Eu-MOF not only exhibited reversible photochromic and electrochromic properties, but also displayed photoluminescent and electroluminochromic properties.

7.
Artigo em Inglês | MEDLINE | ID: mdl-33075564

RESUMO

BACKGROUND & AIMS: Increased vascular permeability (VP) has been indicated to play an important role in the pathogenesis of inflammatory bowel disease (IBD). However, the pathological causes of increased intestinal VP in IBD remain largely unknown. METHOD: Fibrinogen level was measured in dextran sulphate sodium (DSS)-induced colitis and patients with ulcerative colitis. Gly-Pro-Arg-Pro acetate (GPRP), an Fg inhibitor, was used to detect the effect of Fg inhibition on the pathogenesis of DSS-induced colitis, as indicated by tissue damage, cytokine release and inflammatory cell infiltration. Miles assay was used to detect vascular permeability. RESULTS: Through tandem mass tag-based quantitative proteomics, fibrinogen (Fg) was found to be upregulated in the colon of DSS-treated mice, which was consistent with increased Fg level in colon sample of patients with ulcerative colitis. Gly-Pro-Arg-Pro acetate (GPRP), an Fg inhibitor, significantly alleviated DSS-induced colitis as indicated by improvement of body weight loss and mortality. GPRP decreased colonic inflammation and VP in DSS-treated mice. In vivo, Fg enhanced VP as indicated by Miles assay, which was significantly inhibited by GRPR, AKT (serine/threonine kinase 1) inhibitors and low doses of Jasplakinolide which induced actin polymerization, while was dramatically enhanced by Cytochalasin D (an actin polymerization inhibitor). Moreover, activation of AKT was found in vessels of DSS-treated mice. In vitro, Fg induced activation of AKT and depolymerization of microfilament and promoted cell-to-cell disaggregation. Furthermore, inhibition of AKT decreased Fg-induced microfilament depolymerization. CONCLUSIONS: Our findings highlight the importance of Fg in regulating colitis by modulation of VP via activating AKT and subsequent depolymerization of microfilament and suggest Fg as an attractive target for anti-colitis treatment.

8.
Bioorg Med Chem ; 28(24): 115821, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33091789

RESUMO

Podophyllotoxin (PPT), a toxic polyphenol derived from the roots of genus Podophyllum, had been reported with strong inhibition on both normal human cells and tumor cells, which hindered the development of PPT as the candidate antitumor agent. In the present work, multiple NQO1-activatable PPT prodrugs were synthesized for reducing normal cell toxicity and keeping tumor cell toxicity. The antiproliferative activities in vitro showed prodrug 3 was greatly selectively toxic to tumor cells over-expressing NQO1, taxol-resistant A549, hypoxia A549 and HepG2, and lower damage to normal cells in comparison with podophyllotoxin, prodrug 1 and 2. As elucidated by further mechanistic research, prodrug 3 was activated via NQO1 to efficiently while gently produce cytotoxic PPT units and kill tumor cells. In additions, in vivo study revealed that 3 significantly suppressed cancer growth in HepG2 xenograft models without obvious toxicity. Therefore, this NQO1-activatable prodrug delivery system exhibits good biosafety and provides a novel strategy for the development of drug delivery systems.

9.
Artigo em Inglês | MEDLINE | ID: mdl-33075030

RESUMO

INTRODUCTION: Renal trauma grading has a limited ability to distinguish patients who will need intervention after high-grade renal trauma (HGRT). A nomogram incorporating both clinical and radiologic factors has been previously developed to predict bleeding control interventions after HGRT. We aimed to externally validate this nomogram using multi-center data from level-1 trauma centers. METHODS: We gathered data from 7 Level-1 trauma centers. Patients with available initial CT scans were included. Each CT scan was reviewed by two radiologists blinded to the intervention data. Nomogram variables included trauma mechanism, hypotension/shock, concomitant injuries, vascular contrast extravasation (VCE), pararenal hematoma extension, and hematoma rim distance (HRD). Mixed-effect logistic regression was used to assess the associations between predictors and bleeding intervention. The prediction accuracy of the nomogram was assessed using the area under the receiver operating characteristic curve (AUC) and its 95% confidence interval (CI). RESULTS: Overall, 569 HGRT patients were included for external validation. Injury mechanism was blunt in 89%. Using initial CT scans, 14% had VCE and median HRD was 1.7 (0.9-2.6) cm. Overall, 12% underwent bleeding control interventions including 34 angioembolizations and 24 nephrectomies. In the multivariable analysis, presence of VCE was associated with a 3-fold increase in the odds of bleeding interventions (OR: 3.06, 95% CI: 1.44-6.50). Every centimeter increase in HRD was associated with 66% increase in odds of bleeding interventions. External validation of the model provided excellent discrimination in predicting bleeding interventions with an AUC of 0.88 (95% CI: 0.84-0.92). CONCLUSIONS: Our results reinforce the importance of radiologic findings such as VCE and hematoma characteristics in predicting bleeding control interventions after renal trauma. The prediction accuracy of the proposed nomogram remains high using external data. These variables can help to better risk stratify high-grade renal injuries. LEVEL OF EVIDENCE: Prognostic and Epidemiological Study, Level III.

10.
Artigo em Inglês | MEDLINE | ID: mdl-33042860

RESUMO

Objectives: To evaluate metagenomic next-generation sequencing (mNGS) as a diagnostic tool in detecting pathogens from osteoarticular infection (OAI) samples. Methods: 130 samples of joint fluid, sonicate fluid, and tissue were prospectively collected from 92 patients with OAI. The performance of mNGS and microbiology culture was compared pairwise. Results: The overall sensitivity of mNGS was 88.5% (115/130), significantly higher than that of microbiological culture, which had a sensitivity of 69.2% (90/130, p < 0.01). Sensitivity was significantly higher for joint fluid (mNGS: 86.7% vs. microbiology culture: 68.7%, p < 0.01) and sonicate fluid (mNGS: 100% vs. microbiology culture: 66.7%, p < 0.05) samples. mNGS detected 12 pathogenic strains undetected by microbiological culture. Additional pathogens detected by mNGS were Coagulase-negative Staphylococci, Gram-negative Bacillus, Streptococci, Anaerobe, non-tuberculosis mycobacterium, MTCP (p > 0.05), and Mycoplasma (OR = ∞, 95% confidence interval, 5.12-∞, p < 0.001). Additionally, sensitivity by mNGS was higher in antibiotic-treated samples compared to microbiological culture (89.7 vs. 61.5%, p < 0.01). Conclusions: mNGS is a robust diagnostic tool for pathogenic detection in samples from OAI patients, compared to routine cultures. The mNGS technique is particularly valuable to diagnose pathogens that are difficult to be cultured, or to test samples from patients previously treated with antibiotics.

11.
Nan Fang Yi Ke Da Xue Xue Bao ; 40(9): 1288-1294, 2020 Sep 30.
Artigo em Chinês | MEDLINE | ID: mdl-32990238

RESUMO

OBJECTIVE: To investigate the pattern of shikonin-induced cell death in testicular cancer cell I-10 and seminoma TCAM-2 cells and explore the possible mechanism in light of mitochondrial function and glycolysis. METHODS: I-10 cells treated with 0, 1.2, 1.4 and 1.6 µmol/L shikonin and TCAM-2 cells treated with 0, 0.5, 1 and 1.5 µmol/L shikonin were examined for mitochondrial membrane potential and production of reactive oxygen species (ROS) using JC-1 kit and ROS kit, respectively. The levels of intracellular lactic acid in the cells were detected using a lactic acid kit. The inhibitory effect of shikonin on the proliferation of the cells was assessed with MTT assay. The death patterns of the cells were observed by transmission electron microscopy, and annexin V-FITC/PI double staining was used to detect cell apoptosis. Western blotting was used to detect the relative expression levels of the apoptotic proteins Bax, Bcl-2, and cleaved caspase-3, the autophagy- related protein LC3B and glycolysis- related proteins PKM2, GLUT1 and HK2. RESULTS: MTT assay showed that shikonin significantly inhibited the proliferation of I-10 and TCAM-2 cells in a time- and dose-dependent manner (P < 0.05). The IC50 values of shikonin in I-10 cells at 24, 48, and 72 h were 1.8, 1.36 and 1.16 µmol/L, as compared with 2.37, 0.8 and 0.41 µmol/L in TCAM-2 cells, respectively. Shikonin treatment significantly reduced mitochondrial membrane potential, increased ROS levels and lower the level of lactic acid in both I-10 and TCAM-2 cells (P < 0.05). Transmission electron microscopy and annexin V-FITC/PI double staining demonstrated that shikonin induced apoptosis and excessive autophagy in I-10 and TCAM-2 cells (P < 0.05). In both I-10 and TCAM cells, shikonin treatment significantly down- regulated the expressions of Bax, Bcl-2, cleaved caspase-3, PKM2, GLUT1 and HK2, and up-regulated the expression of autophagy-related protein LC3B (P < 0.05). CONCLUSIONS: Shikonin can inhibit the proliferation, induce apoptosis and increase autophagy in both I-10 and TCAM-2 cells probably by affecting energy metabolism of the cells.


Assuntos
Seminoma , Neoplasias Testiculares , Linhagem Celular Tumoral , Glicólise , Humanos , Masculino , Naftoquinonas , Neoplasias Testiculares/tratamento farmacológico
12.
Appl Opt ; 59(24): 7371-7375, 2020 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-32902505

RESUMO

Modeling the mechanical stress birefringence and slow-axis distributions of optical plates is critical for optical lithography systems. In this paper, the distributions of mechanical stress birefringence and the slow axes of optical plates were modeled by the finite element (FE) model, stress optic relations, and the ray-traced Jones matrices method. To validate this model, the load incremental approach was utilized to reduce the disturbance of residual birefringence in mechanical stress birefringence measurement. The measured distributions of birefringence and the slow axis of the optical plate show a good agreement with our numerical simulation results. This model provides a better understanding of simulation of mechanical stress birefringence and provides a reference for optical design and polarization analysis of other optical elements.

13.
Carbohydr Polym ; 249: 116824, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32933671

RESUMO

Non-anticoagulant biological functions of heparin-based drugs have drawn increasing attention. However, the exploration into the non-anticoagulant activities of various low molecular weight heparins was associated with bleeding risks in clinical practice and often led to controversial conclusions due to the structural differences. In this study, we aimed to establish a process to produce a library of heparin derivatives with structural diversity and reduced/abolished anticoagulant activity through the combination of chemical modifications and enzymatic cleavage of heparins. The depolymerization characteristics of various selectively modified heparin derivatives by three heparinases were comprehensively analyzed. The order of periodate treatment and heparinase-I depolymerization was proved to significantly change the structural characteristics of the oligosaccharide products. Finally, among several heparin derivatives that screened in the bleomycin-induced cell apoptosis model, the low molecular weight partially 6-O-/N-desulfated heparins showed the strongest anti-apoptotic activities. This study provided a useful approach for future development of novel heparin-derivative medications.

14.
Bioorg Chem ; 103: 104200, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32890998

RESUMO

Tumor-specific prodrug treatment renders the exclusive delivery of antitumor agents with the lowest untoward effects. In this work, we reported the synthesis and biological assessment of four NQO1-activatable combretastatin A-4 prodrugs constituted by active drug CA-4, different self-immolating linkers, and NQO1-responsive trigger groups. The in vitro antiproliferative activities showed that prodrug 4 displayed greater selective toxicity toward the tumor cells that overexpressed NQO1, taxol-resistant A549 cells, hypoxia-exposed A549 and HepG2 cells, and incurred lower damage to normal cells in comparison with combretastatin A-4, prodrugs 1, 2, and 3. Moreover, based on a mechanistic study, NQO1 triggered prodrug 4 to effectively liberate the parent drug combretastatin A-4 and kill tumor cells. Furthermore, we also demonstrated that prodrug 4 exerted a stronger anticancer effect and greater safety than combretastatin A-4 under in vivo conditions. Hence, from the above results, NQO1 can be used as a specific delivery system for releasing anticancer agents; besides, prodrug 4 can serve as a candidate lead for developing specific anticancer agents.

15.
Sci Rep ; 10(1): 12862, 2020 07 30.
Artigo em Inglês | MEDLINE | ID: mdl-32732994

RESUMO

Recent studies establish a crucial role of the circadian clock in regulating plant defense against pathogens. Whether pathogens modulate host circadian clock as a potential strategy to suppress host innate immunity is not well understood. Coronatine is a toxin produced by the bacterial pathogen Pseudomonas syringae that is known to counteract Arabidopsis defense through mimicking defense signaling molecules, jasmonates (JAs). We report here that COR preferentially suppresses expression of clock-related genes in high throughput gene expression studies, compared with the plant-derived JA molecule methyl jasmonate (MJ). COR treatment dampens the amplitude and lengthens the period of all four reporters tested while MJ and another JA agonist JA-isoleucine (JA-Ile) only affect some reporters. COR, MJ, and JA-Ile act through the canonical JA receptor COI1 in clock regulation. These data support a stronger role of the pathogen-derived molecule COR than plant-derived JA molecules in regulating Arabidopsis clock. Further study shall reveal mechanisms underlying COR regulation of host circadian clock.

16.
Sci Adv ; 6(30): eaay9206, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32766447

RESUMO

Despite advances in hematopoietic stem/progenitor cell (HSPC) transplant for HIV-1-infected patients, the impact of a preexisting HIV-1 infection on the engraftment and clonal repopulation of HSPCs remains poorly understood. We have developed a long terminal repeat indexing-mediated integration site sequencing (LTRi-Seq) method that provides a multiplexed clonal quantitation of both anti-HIV-1 RNAi (RNA interference) gene-modified and control vector-modified cell populations, together with HIV-1-infected cells-all within the same animal. In our HIV-1-preinfected humanized mice, both therapeutic and control HSPCs repopulated efficiently without abnormalities. Although the HIV-1-mediated selection of anti-HIV-1 RNAi-modified clones was evident in HIV-1-infected mice, the organ-to-organ and intra-organ clonal distributions in infected mice were indistinguishable from those in uninfected mice. HIV-1-infected cells showed clonal patterns distinct from those of HSPCs. Our data demonstrate that, despite the substantial impact of HIV-1 infection on CD4+ T cells, HSPC repopulation remains polyclonal, thus supporting the use of HSPC transplant for anti-HIV treatment.

17.
J Tissue Viability ; 2020 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-32768331

RESUMO

This aim of this study was to observe the effect of Yang Yan Qing E Wan (YYQEW) on senescent phenotypes and the expression of ß-catenin and p16INK4a in the hydrogen peroxide (H2O2)-induced premature senescence of normal human skin fibroblasts (NHSFs). Primary normal human skin fibroblasts were randomly divided into a normal group, a blank group, a model group, and a YYQEW group. The cells of the model group and the YYQEW group were exposed to 150 µmol/L H2O2 for 2 h. The morphological changes of the cells were analyzed by microscopy and by kits used to estimate the activities of the senescence-associated ß-galactosidase (SA-ß-gal), reactive oxygen species (ROS), and superoxide dismutase (SOD). The outcomes revealed that dyeing rate proportion of SA-ß-gal was 2.78% ± 0.22% in the normal group, 2.83% ± 0.29% in the blank group, 37.58% ± 2.56% in the model group, and 28.39% ± 0.93% in the YYQEW group. The number of SA-ß-gal positive cells was thus significantly higher in the model group than in the normal or blank group. There were also fewer SA-ß-gal positive cells in the YYQEW group compared with the model group. The expression of ROS and p16INK4a in the model group increased significantly compared with that in the normal or blank groups, while the expression of ROS and p16INK4a in the YYQEW group decreased significantly compared with that in the model group. The expression of SOD and ß-catenin in the model group decreased significantly compared with that in the normal or blank group, and the expression of SOD and ß-catenin in the YYQEW group increased significantly compared with that in the model group. Overall, it was found that YYQEW was able to delay the senescence of NHSFs induced by H2O2 treatment by alleviating oxidative stress and regulating a number of senescence-related molecules, such as ß-catenin and p16INK4a.

18.
Carbohydr Polym ; 247: 116670, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-32829798

RESUMO

With the growing interest in food safety and in environmental protection, it is more attractive to develop novel biodegradable packaging films. In this regard, one new blending film was prepared with curdlan (CD)/polyvinyl alcohol (PVA)/thyme essential oil. Our results demonstrated that the mechanical properties of the blending film were the best when the ratio of the CD and PVA was 4:1. Further, the barrier properties of the film were optimized by incorporating with thyme essential oil. It was proved that not only water vapor permeability was lower, but also the elongation at break was improved, when 2% (w/w) thyme essential oil used. The potential interactions of the film matrix were analyzed by FTIR, XRD and Cryo-scanning electron microscopy. Importantly, both the antioxidant activity and antibacterial activity were improved. Finally, the blending film was employed for the preservation of chilled meat, while the shelf life was extended up to 10 days.

19.
Sensors (Basel) ; 20(16)2020 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-32784818

RESUMO

Eugenol is hepatotoxic and potentially hazardous to human health. This paper reports on a rapid non-destructive quantitative method for the determination of eugenol concentration in curdlan (CD) biofilms by electronic nose (E-nose) combined with gas chromatography-mass spectrometry (GC-MS). Different concentrations of eugenol were added to the film-forming solution to form a series of biofilms by casting method, and the actual eugenol concentration in the biofilm was determined. Analysis of the odor collected on the biofilms was carried out by GC-MS and an E-nose. The E-nose data was subjected to principal component analysis (PCA) and linear discriminant analysis (LDA) in order to establish a discriminant model for determining eugenol concentrations in the biofilms. Further analyses involving the application of all sensors and featured sensors, the prediction model-based partial least squares (PLS) and support vector machines (SVM) were carried out to determine eugenol concentration in the CD biofilms. The results showed that the optimal prediction model for eugenol concentration was obtained by PLS at R2p of 0.952 using 10 sensors. The study described a rapid, non-destructive detection and quantitative method for determining eugenol concentration in bio-based packaging materials.

20.
Artigo em Inglês | MEDLINE | ID: mdl-32778382

RESUMO

BACKGROUND: The purpose of this study was to determine the factors associated with outcomes after reverse total shoulder arthroplasty (RTSA). METHODS: We retrospectively evaluated all RTSAs performed by the senior author between January 1, 2007, and November 1, 2017. We evaluated pain visual analog scale (VAS), Simple Shoulder Test (SST), and American Shoulder and Elbow Surgeons Standardized Shoulder Assessment Form (ASES) scores and complication and reoperation rates at a minimum of 2-year follow-up. We evaluated preoperative and 2-week postoperative radiographs for glenoid inclination (GI), medialization as distance between the center of the humeral head or glenosphere and the line of the deltoid, and distalization via the acromial-greater tuberosity distance. We performed inter- and intrarater reliabilities via intraclass correlation coefficients (ICCs) and conducted a multivariable analysis. RESULTS: We included 230 RTSAs in the analysis, with 70% follow-up at a median of 3.4 years. Reliability was acceptable with all ICCs >.678. Increased postoperative GI was significantly associated with increased VAS pain postoperatively (P = .008). Increased distalization was associated with an increased rate of complications and reoperations (P = .032). Younger age (P = .008), female gender (P = .009), and lower body mass index (BMI) (P = .006) were associated with worse ASES scores. Female gender (P < .001) and lower BMI (P = .039) were associated with worse SST scores. Female gender (P = .013) and lower BMI (P = .005) were associated with worse VAS-pain scores. CONCLUSION: Age, gender, and BMI are associated with outcome after RTSA. In this retrospective analysis of a Grammont-style RTSA, superior inclination is associated with increased pain postoperatively, whereas excessive arm lengthening is associated with increased risk for complication or reoperation.

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