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1.
Mater Sci Eng C Mater Biol Appl ; 106: 110227, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31753352

RESUMO

Organelle-targeting agents are promising in both fundamental and applied biomedicine research, but such materials are very limited. As a curved 2D carbon material, corannulene (Cor) displays an uneven intramolecular electron distribution, producing a large dipole moment that can favor the electrostatic interaction. Based on the large negative mitochondrial membrane potential and the presence of a connection structure between mitochondria and endoplasmic reticulum (ER), we hypothesized that Cor could simultaneously target both mitochondria and ER. Such hypothesis was well validated by using the fluorescence tag-labelled Cor. The co-localization analysis in a model cell line (PC3) revealed a preferred accumulation of Cor in both organelles, as evidenced by a large Pearson correlation coefficient. The large dipole also empowered Cor the ability of controlled production of reactive oxygen species (ROS) upon light irradiation. This feature plus mitochondria targeting of Cor induced depletion of adenosine triphosphate (ATP) and caspase 9/3 activation. The triggered ROS generation in ER caused the calcium dumping in the cytosol, as revealed by a calcium-specific fluorescence probe. A significant degree of apoptosis was induced by Cor as a result of the interplay of dual mitochondria/ER targeting and triggered organelle-specific ROS delivery. This study demonstrated the subcellular targeting ability of Cor for potential ROS-based therapy, and implied that the dipole could be a valuable parameter for efficient design and tailored screening of organelle-targeting materials for various biomedical applications.

2.
Biosens Bioelectron ; 148: 111819, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31678825

RESUMO

In this paper, a sensor based on a magnetic surface molecularly imprinted membrane (MMIP) was prepared for the highly sensitive and selective determination of acetaminophen (AP). Before the experiment, the appropriate functional monomers and solvents required for the polymer were screened, and the molecular electrostatic potentials (MEPs) were calculated by the DFT/B3LYP/6-31 + G method. MMIP with high recognition of AP was synthesized based on Fe3O4@SiO2nanoparticles (NPs) with excellent core-shell structure. Next, a carbon paste electrode (CPE) was filled with a piece of neodymium-iron-boron magnet to make magnetic electrode (MCPE), and MMIP/MCPE sensor was obtained by attaching a printed polymer to the surface of the electrode under the strong magnetic. Due to the stable molecular structure of the electrode surface, the sensor is highly effective and accurate for detection of AP using DPV. The DPV response of the sensor exhibited a linear dependence on the concentration of AP from 6 × 10-8 to 5 × 10-5 mol L-1 and 5 × 10-5 to 2 × 10-4 mol L-1, with a detection limit based on the lower linear range of 1.73 × 10-8 mol L-1(S/N = 3). When used for determination of AP in actual samples, the recovery of the sensor to the sample was 95.80-103.76%, and the RSD was 0.78%-3.05%.

3.
J Nanosci Nanotechnol ; 20(5): 2728-2735, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31635608

RESUMO

Biomass-derived porous carbons are considered as one of the most promising electrode materials for supercapacitors due to their low-cost and natural abundance. In this work, pinecone is used to fabricate biomass N, S, O-doped porous carbon via one-step carbonization process with KOH activation. By optimizing the additive amount of KOH and calcination temperature, the asprepared product shows a high specific surface area and pore volume up to 1593.8 m² g-1 and 0.8582 cm³ g-1, respectively. As an electric double-layer capacitor (EDLC) electrode, the N, S, O-doped porous carbon exhibits a high specific capacitance of 285 F g-1 at 0.5 A g-1 and good rate performance with a capacitance retention of 78.6% from 0.5 to 20 A g-1. Furthermore, the as-assembled symmetric supercapacitor with 6 mol L-1 KOH as electrolyte possesses a promising energy density of 6.34 Wh kg-1 and a power density of 250 W kg-1. Outstanding cycling stability was also demonstrated with 94.4% capacitance retention after 10,000 charge/discharge cycles at 1 A g-1.

4.
Sci Total Environ ; : 134771, 2019 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-31784165

RESUMO

China's Mu Us Desert, located in an energy-rich strategic base of the northwestern Loess Plateau, has acted as a crucial agro-pastoral transition zone for thousands of years. However, the area experienced notable climate and environmental change from 221 BCE to CE 907 (1128 years), which may have profoundly affected its landscape evolution up to modern times. To explore this process and associated driving mechanisms, we conducted a comprehensive study based on a dataset of 882 human archaeological sites (HASs), historical documents, and related environmental data of the Mu Us Desert and its surrounding area (MUDISA). We found that the MUDISA experienced large-scale immigration and an agricultural boom (790 HASs) during the Qin and Han dynasties (221 BCE-CE 220). This coincided with an ecologically favorable environment and may have potentially disturbed the desert's eco-environmental equilibrium. Coinciding with the deteriorating natural conditions, the MUDISA's animal husbandry replaced agriculture as the dominant subsistence economy during the era of disunity (CE 220-581, 33 HASs) and during the Sui and Tang dynasties (CE 581-907, 59 HASs). Although natural and political-economic factors together caused a sharp decline in number and scattering for HASs in the desert, people and livestock were widely distributed and maintained a high population level. This situation, coupled with the dry climate and fragile geographical environment, may have significantly accelerated the climate-driven desertification process from CE 220 to CE 907. This study highlights the long-term human-nature relationship and its impact on historical desertification in the Mu Us Desert, and may shed new light on historical environmental change in arid and/or semi-arid areas in northwest China and globally.

5.
Haematologica ; 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31753928

RESUMO

Thrombotic thrombocytopenic purpura (TTP) is caused by severe deficiency of ADAMTS13 (A13), a plasma metalloprotease that cleaves endothelium-derived von Willebrand factor (VWF). However, severe A13 deficiency alone is often not sufficient to cause an acute episode; additional factors may be required to trigger the disease. Using CRISPR/Cas9, we created and characterized several novel zebrafish lines carrying a null mutation in a13-/-, vwf, and both. We further used these zebrafish lines to test the hypothesis that inflammation that results in neutrophil activation and release of histone/DNA complexes may trigger TTP. As shown, a13-/- zebrafish exhibit increased levels of plasma VWF antigen, multimer size, and ability of thrombocytes to adhere to a fibrillar collagen-coated surface under flow. The a13-/- zebrafish also show an increased rate of forming occlusive thrombi in the caudal venules after FeCl3 injury. More interestingly, a13-/- zebrafish exhibit ~30% reduction in the number of total, immature, and mature thrombocytes with increased fragmentation of erythrocytes. An administration of a lysine-rich histone results in more severe and persistent thrombocytopenia and a significantly increased mortality rate in a13-/- than in wild type (wt) zebrafish. However, both spontaneous and histone-induced TTP in a13-/- zebrafish are rescued by the deletion of vwf. These results demonstrate a potentially mechanistic link between inflammation and the onset of TTP in light of severe A13 deficiency; the novel zebrafish models of TTP may help accelerate our understandings of pathogenic mechanisms and the discoveries of novel therapeutics for TTP and perhaps other arterial thrombotic disorders. .

6.
Nature ; 576(7785): 158-162, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31776509

RESUMO

Features of higher-order chromatin organization-such as A/B compartments, topologically associating domains and chromatin loops-are temporarily disrupted during mitosis1,2. Because these structures are thought to influence gene regulation, it is important to understand how they are re-established after mitosis. Here we examine the dynamics of chromosome reorganization by Hi-C after mitosis in highly purified, synchronous mouse erythroid cell populations. We observed rapid establishment of A/B compartments, followed by their gradual intensification and expansion. Contact domains form from the 'bottom up'-smaller subTADs are formed initially, followed by convergence into multi-domain TAD structures. CTCF is partially retained on mitotic chromosomes and immediately resumes full binding in ana/telophase. By contrast, cohesin is completely evicted from mitotic chromosomes and regains focal binding at a slower rate. The formation of CTCF/cohesin co-anchored structural loops follows the kinetics of cohesin positioning. Stripe-shaped contact patterns-anchored by CTCF-grow in length, which is consistent with a loop-extrusion process after mitosis. Interactions between cis-regulatory elements can form rapidly, with rates exceeding those of CTCF/cohesin-anchored contacts. Notably, we identified a group of rapidly emerging transient contacts between cis-regulatory elements in ana/telophase that are dissolved upon G1 entry, co-incident with the establishment of inner boundaries or nearby interfering chromatin loops. We also describe the relationship between transcription reactivation and architectural features. Our findings indicate that distinct but mutually influential forces drive post-mitotic chromatin reconfiguration.

7.
Mol Med Rep ; 20(5): 4523-4532, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31702044

RESUMO

Betatrophin [also known as lipasin, angiopoietin­like 8 (ANGPTL8), refeeding induced in fat and liver (RIFL), or hepatocellular carcinoma­associated gene TD26], a 22­kDa protein in the angiopoietin­like family, is a liver­derived hormone that promotes pancreatic ß­cell proliferation and lipid metabolism. The aim of the present study was to investigate the effect of recombinant betatrophin on ß­cell regeneration in a neonatal streptozotocin (STZ)­induced diabetic rat model. One­day­old Wistar rats were injected with STZ (100 mg/kg), followed by intraperitoneal administration of betatrophin to the STZ­injected rats for 6 days. Plasma glucose and body weight were monitored. On days 4 and 7, expression levels of pancreatic duodenal homeobox gene­1 (PDX­1), the Bax/B­cell lymphoma­2 (Bcl­2) ratio and plasma insulin were assessed, and the ß­cell proliferation rate was determined. Pancreatic islet area and number were determined at 10 weeks. It was found that betatrophin treatment alleviated STZ­induced hyperglycemia, elevated pancreatic expression levels of Bcl­2, PDX­1, plasma insulin levels and the ß­cell proliferation rate on days 4 and 7. Long­term betatrophin treatment improved glucose tolerance, associated with improved plasma insulin levels and ß­cell mass. These results suggest that early administration of betatrophin promotes ß­cell proliferation in STZ­induced diabetic neonates and prevents the development of diabetes in adults.

8.
Nat Prod Res ; : 1-6, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31686531

RESUMO

A new indoloditerpene (1) and fifteen known compounds (2-16) were isolated from a marine-derived fungus Aspergillus versicolor ZZ761. Structure of the new compound was elucidated as (3 R,9S,12R,13S,17S,18S)-2-carbonyl-3-hydroxylemeniveol based on its HRESIMS data, NMR spectroscopic analyses, the Mosher's method, and ECD calculation. This new indoloditerpene (1) showed antimicrobial activities with MIC values of 20.6 µM against Escherichia coli and 22.8 µM against Candida albicans. Diorcinol (2) and versicolorin B (6) had activities in inhibiting the proliferation of human glioma U87MG and U251 cells with IC50 values of 4.4 and 6.2 µM and 11.3 and 30.5 µM, respectively.

9.
ACS Appl Mater Interfaces ; 11(48): 45199-45206, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31701734

RESUMO

Silicon integration of nanoscale metamaterials is a crucial step toward low-cost and scalable optical-based integrated circuits. Here, a self-assembled epitaxial Au-BaTiO3 (Au-BTO) hybrid metamaterial with highly anisotropic optical properties has been integrated on Si substrates. A thin buffer layer stack (<20 nm) of TiN and SrTiO3 (STO) was applied on Si substrates to ensure the epitaxial growth of the Au-BTO hybrid films. Detailed phase composition and microstructural analyses show excellent crystallinity and epitaxial quality of the Au-BTO films. By varying the film growth conditions, the density and dimension of the Au nanopillars can be tuned effectively, leading to highly tailorable optical properties including tunable localized surface plasmon resonance (LSPR) peak and hyperbolic dispersion shift in the visible and near-infrared regimes. The work highlights the feasibility of integrating epitaxial hybrid oxide-metal plasmonic metamaterials on Si toward future complex Si-based integrated photonics.

10.
J Clin Pharm Ther ; 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31778586

RESUMO

WHAT IS KNOWN AND OBJECTIVE: Myelosuppression, an adverse drug reaction (ADR), often causes medical treatment termination in cancer patients. It has been known that genetic components, such as single-nucleotide polymorphisms (SNPs), influence the risk of myelosuppression at the individual-patient level. However, due to ethnic variation in frequency of genetic polymorphisms, results reported in Caucasian patients may not be generalizable to the Chinese Han population. Until now, few researches on myelosuppression included Chinese Han patients. In this study, we conducted a systematic study of potential biomarkers for docetaxel-induced myelosuppression in Han Chinese patients. METHODS: We examined 61 SNPs in 36 genes that code for drug transporters, metabolism enzymes, nuclear receptors and DNA repair pathway in 110 Chinese Han patients receiving docetaxel-based chemotherapy. Genotyping was conducted using the Sequenom MassARRAY system. Significant SNPs were identified by logistic regression, and gene-gene interactions were investigated by generalized multifactor dimensionality reduction (GMDR) analysis. RESULTS AND DISCUSSION: Our results revealed that 11 SNPs in nine genes (SLC15A1, SLCO1A2, CYP2D6, FMO3, UGT1A1, NAT2, SULT2A1, PXR and HNF4α) were associated with docetaxel-induced myelosuppression. GMDR analyses suggested that a 3-locus model: SLC15A1 rs2297322-PXR rs3732359-FMO3 rs2266782 was an appropriate predictive model of docetaxel-induced myelosuppression (P = .017, Testing Bal.Acc = 0.653, CV Consistency = 10/10). WHAT IS NEW AND CONCLUSION: Our findings suggest multiple novel predictive biomarkers of docetaxel-induced myelosuppression: SLC15A1 rs2297322, PXR rs3732359 and FMO3 rs2266782. These discoveries should help in advancing future personalized therapy of docetaxel-based chemotherapy specific to Chinese Han patients.

11.
Int Immunopharmacol ; 77: 105924, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31678864

RESUMO

As a membrane-permeable derivative of itaconate, dimethyl itaconate (DMI) was recently showed to limit inflammatory response of activated macrophages, and to decrease the generation of reactive oxygen species and reduce cardiac ischemia/reperfusion injury. However, the effect of DMI in the context of cerebral ischemia/reperfusion injury remains unclear. Here, we treated the transient middle cerebral artery occlusion (tMCAO) mice with DMI or saline at the beginning of occlusion, and allowed them to recover for 3 days. We found that DMI obviously decreased the neurologic deficit score. Further, DMI significantly inhibited the toxic conversion of the peri-infarct microglia, and decreased the protein level of interleukin 1ß. The present findings suggest that DMI might be recognized as a promising candidate for the treatment of ischemic stroke.

12.
Neuropharmacology ; 162: 107843, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31704273

RESUMO

Alzheimer's disease (AD), the most common form of dementia, still lacks effective treatment at present. Alpha-asarone (ASA) is the major compound isolated from the Chinese medicinal herb Acorus gramineus. It has been reported to enhance cognitive function in rodent models, yet its mechanism was not fully understood. In this work, we demonstrated that ASA improved the spatial memory, reduced the neuronal injury, and decreased the level of Aß1-42 in the hippocampus of aged rats. The results also showed that ASA had the neuroprotective effects against glutamate toxicity and decreased cytoplasmic calcium level in primary hippocampal neurons. By comparing the multiple properties of ASA and propofol (PPF) via computer modelling, we speculated that ASA may bind to the PPF binding site of type A gamma (γ)-aminobutyric acid receptors (GABAARs). This was further supported by the whole-cell patch-clamp recording. Our results suggested that ASA, as a GABAAR positive allosteric modulator (PAM), can improve cognitive function of aged rats by alleviating the neuronal overexcitation. Furthermore, the binding mode of ASA on GABAAR may lay a foundation for structure-based drug design in AD therapy.

13.
J Headache Pain ; 20(1): 104, 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31711434

RESUMO

BACKGROUND: Functional connectivity (FC) has been used to investigate the pathophysiology of migraine. Accumulating evidence is pointing toward malfunctioning of brainstem structures, i.e., the red nucleus (RN) and substantia nigra (SN), as an important factor in migraine without aura (MwoA). We aimed to identify atypical FC between the RN and SN and other brain areas in patients with MwoA and to explore the association between RN and SN connectivity changes and performance on neuropsychological tests in these patients. METHODS: Resting-state functional magnetic resonance imaging (fMRI) data were obtained from 30 patients with MwoA and 22 age-, sex-, and years of education-matched healthy controls (HC). The FC of the brainstem structures was analyzed using a standard seed-based whole-brain correlation method. The results of the brainstem structure FC were assessed for correlations with other clinical features. RESULTS: Patients with MwoA exhibited reduced left RN-based FC with the left middle frontal gyrus, reduced right RN-based FC with the ipsilateral superior parietal lobe, and increased FC with the ipsilateral cerebellum. Additionally, patients with MwoA demonstrated significantly decreased right SN-based FC with the right postcentral gyrus, left parietal lobule, and left superior frontal gyrus. Hypo-connectivity between the right SN and right postcentral gyrus was negatively correlated with disease duration (r = - 0.506, P = 0.004). Additionally, increased connectivity of the right RN to the ipsilateral cerebellar lobes was positively correlated with the Headache Impact Test-6 scores (r = 0.437, P = 0.016). CONCLUSIONS: The present study suggested that patients with MwoA have disruption in their RN and SN resting-state networks, which are associated with specific clinical characteristics. The changes focus on the regions associated with cognitive evaluation, multisensory integration, and modulation of perception and pain, which may be associated with migraine production, feedback, and development. Taken together, these results may improve our understanding of the neuropathological mechanism of migraine.

14.
Crit Rev Eukaryot Gene Expr ; 29(2): 113-121, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31679266

RESUMO

Endometriosis is a common debilitating gynecologic disease. Almost 10% of reproductive-age women are affected by this disease; they commonly suffer pelvic pain and/or infertility. Early diagnosis of this multifactorial disease remains difficult because its etiology is not clear and the early symptoms are nonspecific. In addition, many reproductive-age women are unwilling to undergo invasive laparoscopic surgery because of the possibility of decreasing fertility. Thus, identifying biomarkers for the early diagnosis of endometriosis a key focus of current research. Long noncoding RNAs (lncRNAs) are a class of noncoding transcripts that have length of > 200 nucleotides and lack protein-coding ability but still influence gene expression in various ways. With advances in genome-wide analysis, researchers have determined that lncRNAs play an important role in many human diseases, particularly tumors. Moreover, the role of lncRNAs in the pathogenesis of endometriosis has been continually recognized. In this review, we discuss the status of current research on dysregulated lncRNAs and their roles in the pathogenesis of endometriosis. We aim to stimulate new investigations toward the identification of lncRNAs as biomarkers for the early diagnosis and therapy of this long-term gynecological disease.

15.
Medicine (Baltimore) ; 98(48): e17984, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31770208

RESUMO

Pediatric patients suffer from chronic pancreatitis (CP), especially those with diabetes mellitus (DM). This study aimed to identify the incidence of and risk factors for DM in pediatric CP.CP patients admitted to our center from January 2000 to December 2013 were assigned to the pediatric (<18 years old) and adult group according to their age at onset of CP. Cumulative rates of DM and risk factors for both groups were calculated and identified.The median follow-up duration for the whole cohort was 7.6 years. In these 2153 patients, 13.5% of them were pediatrics. The mean age at the onset and the diagnosis of CP in pediatrics were 11.622 and 19.727, respectively. DM was detected in 13.1% patients and 31.0% patients in the pediatric group and adult group, respectively. Age at the onset of CP, smoking history, body mass index (BMI), and etiology of CP were identified risk factors for DM in pediatrics.DM was detected in 13.1% pediatric patients. Age at the onset of CP, smoking history, BMI, and etiology of CP were identified risk factors for the development of DM in pediatric CP patients. The high-risk populations were suggested to be monitored frequently. They could also benefit from a lifestyle modification.

16.
Sci Signal ; 12(609)2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31772125

RESUMO

Cyclic GMP-AMP synthase (cGAS) is a major sensor of cytosolic DNA from invading pathogens and damaged cellular organelles. Activation of cGAS promotes liquid-like phase separation and formation of membraneless cytoplasmic structures. Here, we found that cGAS bound G3BP1, a double-stranded nucleic acid helicase involved in the formation of stress granules. Loss of G3BP1 blocked subcellular cGAS condensation and suppressed the interferon response to intracellular DNA and DNA virus particles in cells. Furthermore, an RNA-dependent association with PKR promoted G3BP1 foci formation and cGAS-dependent interferon responses. Together, these results indicate that PKR promotes the formation of G3BP1-dependent, membraneless cytoplasmic structures necessary for the DNA-sensing function of cGAS in human cells. These data suggest that there is a previously unappreciated link between nucleic acid sensing pathways, which requires the formation of specialized subcellular structures.

17.
J Cell Mol Med ; 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31778016

RESUMO

Proteolipid protein 2 (PLP2) is an integral ion channel membrane protein of the endoplasmic reticulum. The protein has been shown to be highly expressed in many cancer types, but its importance in glioma progression is poorly understood. Using publicly available datasets (Rembrandt, TCGA and CGGA), we found that the expression of PLP2 was significantly higher in high-grade gliomas than in low-grade gliomas. We confirmed these results at the protein level through IHC staining of high-grade (n = 56) and low-grade glioma biopsies (n = 16). Kaplan-Meier analysis demonstrated that increased PLP2 expression was associated with poorer patient survival. In functional experiments, siRNA and shRNA PLP2 knockdown induced ER stress and increased apoptosis and autophagy in U87 and U251 glioma cell lines. Inhibition of autophagy with chloroquine augmented apoptotic cell death in U87- and U251-siPLP2 cells. Finally, intracranial xenografts derived from U87- and U251-shPLP2 cells revealed that loss of PLP2 reduced glioma growth in vivo. Our results therefore indicate that increased PLP2 expression promotes GBM growth and that PLP2 represents a potential future therapeutic target.

18.
Spectrochim Acta A Mol Biomol Spectrosc ; 227: 117585, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31734570

RESUMO

We report the preparation of an organic-inorganic hybrid mesoporous material, PHC-SBA-15, derived from the coupling of a pyrene-based derivative PHC and mesoporous SBA-15 silica. Compared with the stable aggregation-induced emission (AIE) properties of PHC, those of PHC-SBA-15 were more promoted and active due to the fixation of PHC and the space limitation in mesoporous SBA-15. The aggregation and disaggregation activities can be tuned by controlling the concentrations in aqueous media and changing the fluorescence color from yellow to blue. In addition to the controllable AIE properties, PHC-SBA-15 was applied for the highly selective and sensitive detection of Hg2+ through the fluorescence quenching of monomeric pyrene in aqueous media. The fluorescence intensity at 395 nm was linearly proportional to that of Hg2+ in the concentration ranges of 0-1.0 × 10-5 and 1.0 × 10-5-10 × 10-5 M, showing a low detection limit of 1.02 × 10-7 M. This work provides an effective strategy for modulating the AIE properties from non-active to active by introducing AIE stable molecule into mesoporous silica material. This method also favors the development of fluorescent sensors for detecting targets with high sensitivity and selectivity in aqueous media with less synthetic difficulties.

19.
Mikrochim Acta ; 186(11): 699, 2019 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-31617008

RESUMO

A vertical flow microarray chip is described that uses core-shell SERS nanotags as tags for ultrasensitive quantification of the tumor markers α-fetoprotein (AFP) and carcinoembryonic antigen (CEA) by detecting the intensity of the specific Raman bands at 592 cm-1. The nanotags warrant high sensitivity, and the use of porous nitrocellulose warrants a high surface-to-volume ratio. The linear dynamic ranges are 0.1 ng mL-1 - 10 µg mL-1 for both AFP and CEA, and the limits of detection) are 0.27 pg mL-1 and 0.96 pg mL-1, respectively. Quantification is rapid and can be performed without preconcentration. Graphical abstract Schematic representation of a vertical flow microarray chip using AuNBA@Ag SERS nanotags (where NBA stands for Nile blue A) as labels for rapid, ultrasensitive, and simultaneous detection of tumor biomarkers CEA and AFP.

20.
ACS Appl Mater Interfaces ; 11(43): 39594-39602, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31577410

RESUMO

Photodynamic therapy (PDT) has attracted great attention as an alternative tumor treatment method. Unfortunately, it suffers from some limitations like poor targeting capability and insufficient therapeutic efficiency caused by tumor hypoxia. In this work, we introduce a novel O2-evolving PDT nanoparticle for homologous cancer cell targeting as well as dual-mode imaging [i.e., magnetic resonance imaging (MRI) and fluorescence imaging]. Specifically, the nanostructure consists of a MnO2 nanosheet-coated metal-organic framework core and cancer cell membrane shell (defined as CM-MMNPs). The MnO2 layer displays H+ and H2O2 responsiveness, which can produce O2 to enhance O2-mediated singlet oxygen (1O2) generation for PDT. Moreover, the resulted Mn2+ can also be used as an optimal MRI contrast agent. The introduction of cell membrane and membrane proteins endow the CM-MMNPs with good stability and integrity in the process of cellular endocytosis, as well as strong homologous cell-targeting ability. This multifunctional nanoparticle has the potential to overcome the hypoxia of cancer cells in PDT, and provides a new paradigm for tumor targeting, detection, and therapy, which is promising for biomedical applications in the future.

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