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1.
Artigo em Inglês | MEDLINE | ID: mdl-36731170

RESUMO

The Ginzburg-Landau theory, which was introduced to phenomenologically describe the destruction of superconductivity by a magnetic field at the beginning, has brought up much more knowledge beyond the original one as a mean-field theory of thermodynamics states. There the complex order parameter plays an important role. Here we propose a macroscopic theory to describe the features of ferroelectrics by a two-component complex order parameter coupled to nonabelian gauge potentials that provide more freedom to reflect interplays between different measurables. Within this theoretical framework, some recently discovered empirical static and time-independent phenomena, such as vortex, anti-vortex, sprial orders can be obtained as solutions for different gauge potentials. It is expected to bring in a new angle of view with more elucidation than the traditional one that takes the polarization as order parameter.

2.
Plant Physiol ; 2023 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-36732887

RESUMO

Red-skinned pears (Pyrus L.) are preferred to consumers for their attractive color and abundant anthocyanins. Pyrus ETHYLENE RESPONSE FACTOR 3 (PyERF3) positively regulates anthocyanin biosynthesis through interacting with Pyrus myeloblastosis family 4 (PyMYB114) and Pyrus basic helix-loop-helix 3 (PybHLH3) in red-skinned pears. However, the role of APETALA2/ethylene response factors (AP2/ERFs), which negatively regulate anthocyanin biosynthesis, remains unclearin red-skinned pears. Here, we validated that two AP2/ERFs, PyERF4.1 and PyERF4.2, screened from the transcriptome data of 'Starkrimson' pear (Pyrus communis L.) and its green mutant, inhibit anthocyanin biosynthesis in transgenic pear calli, as well as in overexpression and gene edited tomato (Solanum lycopersicum) fruits. Meanwhile, the co-transformation of PyERF4.1/PyERF4.2 with PyERF3-PyMYB114-PybHLH3 inhibited anthocyanin biosynthesis in pear fruits and strawberry (Fragaria vesca) receptacles. Further assays showed that PyMYB114 activated the transcription of PyERF4.1/PyERF4.2; PyERF4.1/PyERF4.2 then interacted with PyERF3 to affect the stability of the PyERF3-PyMYB114-PybHLH3 complex, thereby inhibiting the transcription of the anthocyanin biosynthesis gene Pyrus anthocyanidin synthase (PyANS). Furthermore, deletion of the ERF-associated-amphiphilic repression (EAR) motif eliminated the inhibitory effect of PyERF4.1/PyERF4.2 on anthocyanin biosynthesis, and a mutation of the PyERF4.2-EAR motif (LxLxM to LxLxL) strengthened the inhibitory effect, demonstrating that the EAR motif is indispensable for the inhibitory effect of PyERF4.1/PyERF4.2 on anthocyanin biosynthesis in pears. Our study has shed light on a feedback regulatory loop mechanism that balances the excessive accumulation of anthocyanins in red-skinned pears, providing insights into the regulatory mechanism of anthocyanin biosynthesis and the regulatory network of coloration in red-skinned pears.

3.
Plant Physiol ; 2023 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-36732924

RESUMO

Hydrogen sulfide (H2S) is a gaseous signaling molecule reported to play multiple roles in fruit ripening. However, the molecular mechanisms underlying H2S-mediated delay in fruit ripening remain to be established. Here, the gene encoding a WRKY transcription factor (TF), WRKY71, was identified as substantially upregulated in H2S-treated tomato (Solanum lycopersicum) via transcriptome profiling. Expression of WRKY71 was negatively associated with that of CYANOALANINE SYNTHASE1 (CAS1). Transient and stable genetic modification experiments disclosed that WRKY71 acts as a repressor of the tomato ripening process. CAS1 appears to play an opposite role, based on the finding that the ripening process was delayed in the cas1 mutant and accelerated in CAS1-OE tomatoes. Dual-luciferase reporter assay (DLR), yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), and transient transformation experiments showed that WRKY71 bond to the CAS1 promoter and suppressed its activation. Moreover, the persulfidation of WRKY71 enhanced its binding ability to the CAS1 promoter. Data from luciferase complementation and Y2H assays confirmed that WRKY71 interacts with a BOI-related E3 ubiquitin-protein ligase 3 (BRG3) and is ubiquitinated in vitro. Further experiments showed that modification of BRG3 via persulfidation at Cys206 and Cys212 led to reduced ubiquitination activity. Our findings support a model whereby BRG3 undergoes persulfidation at Cys206 and Cys212, leading to reduced ubiquitination activity and decreased interactions with the WRKY71 transcript, with a subsequent increase in binding activity of the persulfidated WRKY71 to the CAS1 promoter, resulting in its transcriptional inhibition and thereby delayed ripening of tomatoes. Our collective findings provide insights into a mechanism of H2S-mediated regulation of tomato fruit ripening.

4.
J Occup Environ Med ; 2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36728160

RESUMO

BACKGROUND: Combined effect of elevated blood pressure and occupational noise exposure on hearing loss has rarely been evaluated among Chinese population. METHODS: This cross-sectional study was conducted in 242,811 participants. Logistic regression model was performed to estimate the independent and combined associations. RESULTS: Compared with participants without occupational noise exposure, the risk of bilateral high-frequency hearing loss (BHFHL) was significantly higher for noise exposure ≥10 years (OR = 1.29, 95%CI = 1.23-1.35). Compared with no hypertension, participants with grade 1 hypertension had higher risk of BHFHL in all age groups (OR = 1.14, 95%CI = 1.09-1.20). As to the combined effect, the highest BHFHL risk was found in males (OR = 1.51, 95%CI = 1.37-1.67), especially among participants with grade 1 hypertension aged >50 (OR = 1.65, 95%CI = 1.46-1.88). CONCLUSIONS: Elevated blood pressure may synergistically influence hearing loss combined with occupational noise exposure.

5.
Chem Biodivers ; 2023 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-36728645

RESUMO

Five new ent-pimarane diterpenes (1-5) and five known analogues (6-10) were isolated from the aerial parts of Siegesbeckia pubescens. Their structures, including absolute configurations, were determined by comprehensive spectroscopic methods especially 1D and 2D NMR and quantum chemical electronic circular dichroism calculations. All the isolated compounds were evaluated for their cytotoxicity against human BT549, A549 and H157 cancer cell lines. Among them, compounds 1 and 2 showed mild cytotoxicity against lung cancer cell lines H157 with IC50 values of 16.35 ± 2.59 and 18.86 ± 4.83 µM, respectively.

6.
J Orthop Surg Res ; 18(1): 77, 2023 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-36721188

RESUMO

BACKGROUND: Non-vascularized bone grafting (NVBG) has demonstrated to treat osteonecrosis of the femoral head (ONFH). There are a number of articles updating the use of NVBG to treat the ONFH, but the percentage of patients subsequently undergoing a total hip arthroplasty (THA) is controversial. METHODS: Several electronic databases, including PubMed, Embase, Web of Science, and Cochrane databases, were searched to find studies using NVBG to treat ONFH. The pooled rate and 95% confidence interval (CI) were used to assess the conversion rate to THA after NVBG. In addition, we performed subgroup, sensitivity, and publication bias analysis. RESULTS: A total of 37 studies describing 2599 hips were included. The mean weighted follow-up time was 50.5 months and the mean age at surgery was 36.3 years. The conversion rate to THA after NVBG was 21% (95%CI: 17% to 25%), and subgroup analyzes indicated lightbulb, trapdoor and Phemister techniques incidences with THA of 15%, 19%, and 24%, respectively. CONCLUSIONS: This study preliminarily obtained the general trend of the survival rate of NVBG patients, but these results should be interpreted cautiously. Pooled results from 2599 hips and of these nearly 80% with early stage of osteonecrosis, showed that approximately 21% of patients underwent a THA following NVBG. NVBG treatment for patient with ONFH appears to defer or at least delay the need for THA.


Assuntos
Artroplastia de Quadril , Necrose da Cabeça do Fêmur , Humanos , Adulto , Cabeça do Fêmur/cirurgia , Bases de Dados Factuais , Necrose da Cabeça do Fêmur/cirurgia
7.
Ann Gen Psychiatry ; 22(1): 3, 2023 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-36721207

RESUMO

BACKGROUND: Sleep disorders are common during the outbreak of pandemic diseases, and similar disorders are noted in hospitalized COVID-19 patients. It is valuable to explore the clinical manifestations and risk factors for sleep disorders in COVID-19 patients. METHODS: Inpatients with COVID-19 were enrolled. Detailed clinical information was collected, and sleep quality was assessed by PSQI. Patients were divided into a sleep disorder group and a normal group based on a PSQI ≥ 7, and the clinical features were compared between the groups. RESULTS: Fifty-three patients were enrolled, and 47.2% presented sleep disorders. Sleep disorders were associated with older age (> 50), anemia and carbon dioxide retention. Furthermore, factors associated with abnormal component scores of the PSQI were: (1) patients with older age were more likely to have decreased sleep quality, prolonged sleep latency, decreased sleep efficiency, sleep disturbances, and daytime dysfunction; (2) decreased sleep quality and prolonged sleep latency were associated with dyspnea, whereas carbon dioxide retention and more lobes involved in chest CT were associated with prolonged sleep latency; (3) decreased sleep efficiency was more prevalent in patients with anemia. CONCLUSIONS: Sleep disorders were prevalent in patients during the acute phase of COVID-19, and many risk factors (older age, anemia, carbon dioxide retention, the number of lobes involved in chest CT, and dyspnea) were identified. It is important to assess the presence of sleep disorders in patients to provide early intervention.

8.
Adv Mater ; : e2209469, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36722205

RESUMO

Reversible protonic ceramic electrochemical cells (R-PCECs) are emerging as ideal devices for highly efficient energy conversion (generating electricity) and storage (producing H2 ) at intermediate temperatures (400-700°C). However, their commercialization is largely hindered by the development of highly efficient air electrodes for oxygen reduction and water-splitting reactions. Here we report our findings in the design of a highly active and durable air electrode: high-entropy Pr0.2 Ba0.2 Sr0.2 La0.2 Ca0.2 CoO3-δ (HE-PBSLCC), which exhibits impressive activity and stability for oxygen reduction and water-splitting reactions, as confirmed by electrochemical characterizations and structural analyses. When used as an air electrode of R-PCEC, the HE-PBSLCC achieves encouraging performances in dual modes of fuel cells (FC) and electrolysis cells (EC) at 650°C, demonstrating a maximum power density of 1.51 W cm-2 in FC mode, and a current density of -2.68 A cm-2 at 1.3 V in EC mode. Furthermore, the cells display good operational durabilities in FC and EC modes for over 270 h and 500 h, respectively, and promising cycling durability for 70 h with reasonable Faradaic efficiencies. This study offers an effective strategy for the design of active and durable air electrodes for efficient oxygen reduction and water-splitting. This article is protected by copyright. All rights reserved.

9.
Curr Med Chem ; 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36683315

RESUMO

BACKGROUND: Compounds that target tumor epigenetic events are likely to constitute a prominent strategy for anticancer treatment. Histone deacetylase inhibitors (HDACis) have been developed as prospective candidates in anticancer drug development, and currently, many of them are under clinical investigation. We assessed the anticancer efficacy of a now hydroxamate-based HDACi, YF-343, in triple-negative breast cancer development and studied its potential mechanisms. METHODS: YF-343 was estimated as a novel HDACi by the HDACi drug screening kit. The biological effects of YF-343 in a panel of breast cancer cell lines were analyzed by Western blot and flow cytometry. YF-343 exhibited notable cytotoxicity, promoted apoptosis, and induced cell cycle arrest. Furthermore, it also induced autophagy, which plays a pro-survival role in breast cancer cells. RESULTS: The combination of YF-343 with an autophagy inhibitor chloroquine (CQ) significantly suppressed breast tumor progression as compared to the YF-343 treatment alone both in vitro and in vivo. Mechanistically, the molecular mechanism of YF-343 on autophagy was elucidated by gene chip expression profiles, qPCR analysis, luciferase reporter gene assay, chromatin immunoprecipitation assays, immunohistochemical analysis, and other methods. E2F7, a transcription factor, promoted the expression of ATG2A via binding to the ATG2A promoter region and then induced autophagy in triple-negative breast cancer cells treated with YF-343. CONCLUSION: Our studies have illustrated the mechanisms for potential action of YF-343 on tumor growth in breast cancer models with pro-survival autophagy. The combination therapy of YF-343 and CQ maybe a promising strategy for breast cancer therapy.

10.
Med Phys ; 2023 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-36688628

RESUMO

PURPOSE: To develop a deep learning network that treats the three-dimensional respiratory motion signals as a whole and considers the inter-dimensional correlation between signals of different directions for accurate respiratory tumor motion prediction. METHODS: We propose a deep learning framework, named as LSTM-Global Temporal Convolution-External Attention Network (LGEANet). In LGEANet, we first feed each of the univariate time series into the Long Short-Term Memory (LSTM) module respectively and utilize the strength of the global temporal convolutional layer to discover the temporal pattern of the univariate signals from hidden states of the LSTM. Then, External attention is adopted to capture the dynamic dependence of the multiple time series. Also, a traditional autoregressive linear model in parallel to the non-linear neural network part was integrated to mitigate the scale insensitivity of the networks. A total of 304 motion traces for 31 patients are acquired from a public dataset in the experiments and four representative cases were selected for model evaluation. The respiratory signals were sampled at intervals of about 37.5 ms (26 frames per second) for an average duration of 71 min. RESULTS: The proposed LGEANet achieved better performance with higher empirical correlation coefficient value (CORRs) and lower mean absolute error value (MAEs) and relative squared error value (RSEs) than other investigated models. For the four representative datasets, when the response time is less than 231 ms, the model can achieve CORRs more than 0.96. And the averaged position error reduction by using the proposed model was about 67% in the superior-inferior (SI) direction, 41% in the anterior-posterior (AP) direction and 38% in the right-left (RL) direction compared to that without prediction. The proposed network achieved the greatest error reduction in the SI direction, which is the main direction of tumor motion. CONCLUSIONS: The LGEANet achieves promising performance in minimizing the prediction error due to system latencies during real-time tumor motion tracking. This article is protected by copyright. All rights reserved.

11.
Int Immunopharmacol ; 115: 109710, 2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36652757

RESUMO

BACKGROUND: Chronic allograft dysfunction (CAD) is a common cause of allograft loss in kidney transplant recipients (KTRs). Our previous study found that elevated serum soluble T cell immunoglobulin mucin-3 (sTim-3) was positively associated with the severity of CAD in KTRs. sTim-3 was reported to be generated from ADAM10/ADAM17-mediated ectodomain shedding of membrane Tim-3 (mTim-3) in humans. However, whether mTim-3 shedding-related molecules participate in the progression of CAD remains unknown. Here, we explored the relationships between different forms of Tim-3, including mTim-3 on different peripheral blood cell subsets, serum and urine sTim-3, and ADAM10/17 expression and active status to investigate their roles in CAD. METHODS: 63 KTRs with stable grafts, 91 KTRs with CAD and 42 healthy controls (HCs) were enrolled. Total Tim-3, pADAM10/17 and mADAM10/17 proteins were semiquantified by western blot. Serum and urine sTim-3 concentrations were determined by ELISA. mTim-3 and ADAM10/17 expression on leukocyte subpopulations was determined by flow cytometry. RESULTS: The KTR groups displayed significantly higher levels of urine sTim-3 pg/µmol creatinine than the HC group, while no difference was found between the two KTR groups. KTRs with CAD presented reduced nonactive pADAM10 protein but unaltered active mADAM10 when compared to the Stable group; no difference was found between the KTR groups regarding total Tim-3 and p/m ADAM17 protein levels. In addition, the CAD group showed lower mTim-3 expression on BDCA3+ DC than the Stable group; no other difference was observed in its expression on B, T, NK, NKT, monocyte subsets and other DC subsets among groups. With the deterioration of allograft function, ADAM10 expression densities on classical, intermediate, and non-classical monocytes were significantly decreased. Correlation analyses revealed that eGFR and serum sTim-3 exhibited weak to modest correlations with ADAM10 on monocyte and DC subsets. CONCLUSIONS: Our data indicated that ADAM10, especially its decreased expression on monocytes, may play an important role in the progression of CAD in KTRs. However, whether there is an interaction between ADAM10 and mTim-3 in the pathogenesis of CAD in KTRs needs to be further studied.

12.
ACS Omega ; 8(2): 1864-1875, 2023 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-36687025

RESUMO

Brittleness and poor tensile/flexural properties restrict the application of calcium aluminate cement (CAC) in oil and gas wells. Reinforcing CAC with fibers is an effective method for improving its strength and toughness and overcoming the shortcomings of its mechanical properties. In this article, as an auxiliary cementing material, slag does not affect the thickening time of CAC. After adding slag, the cement slurry meets the thickening time during cementing construction, and basalt fiber is selected as the toughening material. The enhancement effect of basalt fiber on the mechanical properties of CAC slag composites is studied, including the evaluation of the macroscopic mechanical properties and microstructure at a high temperature (500 °C). The optimum composition of basalt and fiber-reinforced CAC was determined. Basalt fibers were added to CAC at different contents of 0, 0.1, 0.2, 0.3, 0.4, and 0.5% based on the weight of the cement. All the results showed that the introduction of basalt fibers could significantly enhance the strength of the cement at high temperatures. Compared with the control samples, an additional increase in the compressive and tensile strengths of the samples of 35.1 and 85.2%, respectively, was achieved at high temperature with approximately 0.4% fiber content. Plasma treatment further improved the reinforcing effect of the basalt fibers, where the high-temperature compressive and tensile strengths of the samples increased from 28.88 and 1.52 to 35.23 and 1.87 MPa, respectively, an increase of 21.98 and 20.6%, respectively, compared with the untreated basalt fibers. When the cement paste is cured by simulated curing for 28 d, the high-temperature compressive strength and tensile strength with plasma modification increased from 28.26 and 1.5 to 29.1 and 2.15 MPa, respectively, an increase of 3.0 and 43.3%, respectively. The structure of the formed hydrates was studied using scanning electron microscopy. Furthermore, toughening of the basalt fiber-reinforced CAC-based composites resulted mainly from crack bridging and fiber pull-out.

13.
J Food Sci ; 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36704896

RESUMO

Kudzu root (Pueraria lobata) is well known for its traditional use as a medicinal food homologous plant in China. Three varieties of kudzu roots, such as Gange-1, Gange-2, and Gange-6, are commonly used. Nowadays, kudzu starch (KS) is commercially available as satiating foods or product ingredients. Differentiation and selection of the variety are important components of quality control for KS-based products. Thus, the present work was aimed at comparing the physicochemical properties, such as thermodynamic properties, pasting properties, solubility, swelling, as well as the structural characteristics of the starches extracted from the three varieties of kudzu roots. The results show that KS-6 has a higher content of functional ingredients thus can be used as an ideal functional starch. However, KS-6 has a higher amylopectin:amylose ratio of 4.65, resulting in a better solubility, higher transition temperature, and higher gelatinization enthalpy. KS-2 showed lower transition temperature and gelatinization enthalpy, as well as higher peak viscosity, through viscosity, and final viscosity. KS-1 could result in a soft texture after pasting. The appropriate variety of KS should be differentiated and selected according to application scenarios. This study provided valuable insights into the potential use of different KS in the food and nonfood industries. PRACTICAL APPLICATION: 1. KS-1 was found to be suitable for use as a food supplement. 2. KS-6 has the highest nutritional value. 3. They can be used as a substitute for other similar starches.

14.
BMC Plant Biol ; 23(1): 55, 2023 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-36698063

RESUMO

Drought is a major abiotic stress to rice (Oryza sativa) during growth. Ideal Plant Architecture (IPA1), the first cloned gene controlling the ideal plant type in rice, has been reported to function in both ideal rice plant architecture and biotic resistance. Here, we report that the IPA1/OsSPL14, encoding a transcriptional factor, positively regulates drought tolerance in rice. The IPA1 is constitutively expressed and regulated by H2O2, abscisic acid, NaCl and polyethylene glycol 6000 treatments in rice. Furthermore, the IPA1-knockout plants showed much greater accumulation of H2O2 as measured by 3,3'-diaminobenzidine staining in leaves compared with WT plants. Yeast one-hybrid, dual-luciferase and electrophoretic mobility shift assays indicated that the IPA1 directly activates the promoter of SNAC1. Expression of SNAC1 is significantly down-regulated in IPA1 knockout plants. Further investigation indicated that the IPA1 plays a positive role in drought-stress tolerance by inducing reactive oxygen species scavenging in rice. Together, these findings indicated that the IPA1 played important roles in drought tolerance by regulating SNAC1, thus activating the antioxidant system in rice.


Assuntos
Oryza , Oryza/metabolismo , Plantas Geneticamente Modificadas/genética , Peróxido de Hidrogênio/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Estresse Fisiológico/genética , Secas , Regulação da Expressão Gênica de Plantas
15.
J Neurosci ; 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36627208

RESUMO

It is well established that Ryanodine receptors (RyanR) are overactive in Alzheimer's disease (AD) and it has been suggested that inhibition of RyanR is potentially beneficial for AD treatment. In the present study we explored a potential connection between basal RyanR activity and autophagy in neurons. Autophagy plays an important role in clearing damaged organelles and long-lived protein aggregates, and autophagy dysregulation occurs in both AD patients and AD animal models. Autophagy is known to be regulated by intracellular calcium (Ca2+) signals, and our results indicated that basal RyanR2 activity in hippocampal neurons inhibited autophagy through activation of calcineurin (CaN) and resulting inhibition of AMPK-ULK1 pathway. Thus, we hypothesized that increased basal RyanR2 activity in AD may lead to inhibition of neuronal autophagy and accumulation of ß-amyloid. To test this hypothesis, we took advantage of the RyanR2-E4872Q knock-in mouse model (EQ) in which basal RyanR2 activity is reduced due to shortened channel open time. We discovered that crossing EQ mice with the APPKI and APPPS1 mouse models of AD (both males and females) rescued amyloid accumulation and LTP impairment in these mice. Our results revealed that reduced basal activity of RyanR2-EQ channels disinhibited the autophagic pathway and led to increased amyloid clearance in these models. These data indicated a potential pathogenic outcome of RyanR2 overactivation in AD and also provided additional targets for therapeutic intervention in AD.One Sentence Summary:Basal activity of ryanodine receptors controls neuronal autophagy and contributes to development of AD phenotypeSignificance Statement:It is well established that neuronal autophagy is impaired in Alzheimer's disease (AD). Our results suggest that supranormal calcium (Ca2+) release from endoplasmic reticulum contributes to inhibition of autophagy in AD and that reduction in basal activity of type 2 ryanodine receptors (RyanR2) disinhibits the neuronal autophagic pathway and led to increased amyloid clearance in AD models. Our findings directly link neuronal Ca2+ dysregulation with autophagy dysfunction in AD point to additional targets for therapeutic intervention.

16.
Arch Oral Biol ; 147: 105615, 2023 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-36630765

RESUMO

OBJECTIVE: Head and neck squamous cell carcinoma (HNSCC), is one of the malignant tumors with high recurrence and metastasis. The family with sequence similarity (FAM) of non-coding RNAs promoted tumorigenesis and metastasis. But so far, long non-coding RNA (lncRNA) FAM239A's function in HNSCC regulation remains unclear. This study aimed to explore the lncRNA FAM239A function and regulation mechanism in HNSCC cell proliferation and migration. DESIGN: The expression level of lncRNA FAM239A and tyrosine phosphatase Src homology 2 domain-containing phosphatase 2 (SHP2) in HNSCC tumor tissue was tested by quantitative polymerase chain reaction. The cell proliferation and migration were tested by cell counting kit 8, kinetic live cell assay, and wound healing assay. The differential expression of SHP2 and immune infiltration in HNSCC were analyzed in the tumor immune estimation response and human protein atlas databases. And the survival analysis of SHP2 in HNSCC was analyzed in the gene expression profiling interactive analysis 2 databases. The SHP2 expression was tested by western blotting when lncRNA FAM239A overexpression and knockdown. RESULTS: LncRNA FAM239A and SHP2 were ectopically expressed in HNSCC tumor tissue. Cell proliferation and wound healing assays showed that lncRNA FAM239A promoted tumor cell proliferation and migration. SHP2 was overexpressed in HNSCC tumor tissue by database analyses, and the higher SHP2 expression caused poorer overall survival and disease-free survival in HNSCC patients. SHP2 expression was positively regulated by lncRNA FAM239A. CONCLUSIONS: LncRNA FAM239A promoted HNSCC cell proliferation and migration through upregulating SHP2 expression, which potentially provided new regulators for HNSCC.

17.
Foods ; 12(1)2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36613392

RESUMO

The health benefits of Vaccinium bracteatum are well recorded in ancient Chinese medical books and were also demonstrated by modern researches. However, the relationship between its beneficial functions and specific chemical constituents has not been fully characterized. This study investigated the bioactive small-molecule constituents in the leaves of V. bracteatum, which afforded 32 compounds including ten new ones (1-9) and ten pairs of enantiomers (9-18). Their structures with absolute configurations were elucidated by spectroscopic methods, especially nuclear magnetic resonance (NMR) and electronic circular dichroism (ECD) analyses, with 1-4 bearing a novel revolving-door shaped scaffold. While half-compounds exhibited decent antioxidant activity by scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, all except 19 and 20 exerted significant capturing activity against diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radicals. In addition, the new iridoids 1, 5, 6, and 7 exerted apparent neuroprotective activity toward PC12 cells, with 1 being comparable to the positive control, and selective compounds also displayed anti-diabetic and anti-inflammatory properties by inhibiting α-glucosidase and NO production, respectively. The current work revealed that the bioactive small-molecule constituents could be closely related to the functional food property of the title species.

18.
Artigo em Chinês | MEDLINE | ID: mdl-36597365

RESUMO

Objective:To explore the application value of three dimensional(3D) visualization in the endoscopic thyroidectomy. Methods:Fifty patients with thyroid papillary carcinoma confirmed by preoperative fine needle aspiration(FNA) were randomly divided into the combined group (20 cases) and the simple operation group (30 cases). Endoscopic thyroid surgery combined with three-dimensional visualization model was used in the combined group. Simple operation group was treated with simple endoscopic thyroid surgery. The communication time and communication satisfaction, intraoperative blood loss, operation time and postoperative complications between the two groups were compared. Results:The communication time with patients in the combined group and the simple operation group before operation was (23.05±6.83) min and (28.83±8.57) min. The communication time in the combined group was shorter, and the patients' satisfaction with disease awareness was higher after communication. There was a statistically significant difference between the two groups (P<0.05). The operation time of combined group and simple operation group was (104.30±13.06) min and (130.46±17.01) min respectively, and the operation time of combined group was shorter (P<0.05). The intraoperative bleeding volume of combined group and simple operation group was (12.80±6.10) mL and (17.60±5.19) mL , and the combined group had less intraoperative bleeding volume (P<0.05). There was no significant difference in postoperative complications between the two groups (P>0.05). Conclusion:3D visualization technology will benefit to improve the efficiency of doctor-patient communication, enhance the safety of the endoscopic thyroidectomy, and then accelerate the postoperative rehabilitation of patients, which worthy of clinical promotion and practice.


Assuntos
Neoplasias da Glândula Tireoide , Tireoidectomia , Humanos , Tireoidectomia/métodos , Imageamento Tridimensional , Neoplasias da Glândula Tireoide/cirurgia , Endoscopia/métodos , Complicações Pós-Operatórias/cirurgia , Estudos Retrospectivos
19.
Anticancer Drugs ; 2023 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-36602434

RESUMO

INTRODUCTION: Currently, there are few reports of patients with locally advanced lung cancer achieving a clinical complete response by medical treatment. Preoperative neoadjuvant immunotherapy combined with chemotherapy is an option for patients with unresectable, locally advanced nonsmall cell lung cancer (NSCLC) which is of great potential, and may change traditional treatment paradigms. There are relatively few large-scale, high-quality randomized-controlled trials yet, and limitations such as short postoperative follow-up period and immature disease-free survival and overall survival data still persist. Thus, evidence-based medical evidence is urgently needed. It is worthy to explore the further treatment of patients who achieved complete response after initial treatment, though lacking of evidence by now. CASE PRESENTATION: We report a stage IIIA lung squamous cell carcinoma case who achieved a major pathologic remission after neoadjuvant treatment with tislelizumab and chemotherapy. CONCLUSION: Our case study contributes to the existing evidence on the feasibility, efficacy and safety of neoadjuvant immunotherapy combined with chemotherapy in locally advanced unresectable NSCLC.

20.
J Fluoresc ; 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36622493

RESUMO

A novel fluorescent probe SHK for Zn2+ detection was designed based on the hydrazone Schiff base, successfully synthesized by Suzuki coupling and condensation reactions. The probe SHK in DMSO/H2O showed extremely weak fluorescence. However, the solution exhibited an intensive yellow-green emission with the introduction of Zn2+. In contrast, negligible fluorescence change was observed when other metal ions were added, suggesting a high selectivity of SHK for Zn2+ detection. The Job's Plot analysis revealed that a 1:1 stoichiometric adduct SHK-Zn2+ formed during the Zn2+ sensing. The binding constant of the complex was determined to be 184 M- 1, and the detection limit for Zn2+ was calculated to be 112 µM. Moreover, the probe SHK achieved selective fluorescence sensing for Zn2+ on test strips, which guaranteed its practical application prospect.

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