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1.
Bioact Mater ; 9: 29-43, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34820553

RESUMO

Inflammatory responses of nucleus pulposus (NP) can induce imbalanced anabolism and catabolism of extracellular matrix, and the cytosolic dsDNA accumulation and STING-NF-κB pathway activation found in NP inflammation are considered as fairly important cause of intervertebral disc (IVD) degeneration. Herein, we constructed a siSTING delivery hydrogel of aldehyde hyaluronic acid (HA-CHO) and poly(amidoamine) PAMAM/siRNA complex to intervene the abnormal STING signal for IVD degeneration treatment, where the formation of dynamic Schiff base bonds in the system (siSTING@HPgel) was able to overcome the shortcomings such as low cellular uptake, short half-life, and rapid degradation of siRNA-based strategy. PAMAM not only formed complexes with siRNA to promote siRNA transfection, but also served as dynamic crosslinker to construct hydrogel, and the injectable and self-healing hydrogel efficiently and steadily silenced STING expression in NP cells. Finally, the siSTING@HPgel significantly eased IVD inflammation and slowed IVD degeneration by prolonging STING knockdown in puncture-induced IVD degeneration rat model, revealing that STING pathway was a therapeutic target for IVD degeneration and such novel hydrogel had great potential for being applied to many other diseases for gene delivery.

2.
J Ethnopharmacol ; 283: 114676, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34562564

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The herbal formula Guizhi Fuling Wan is one common remedy for treating uterine fibroids (UFs) and the relevant symptoms in Traditional Chinese Medicine (TCM). Previous systematic reviews showed that Guizhi Fuling Formula appeared to have additional benefit based on mifepristone treatment in reducing volume of fibroids. AIM OF STUDY: To study the efficacy and safety of the conventional dose of a modified herbal formula Guizhi Fuling Wan in patients with symptomatic uterine fibroids in comparison with a sub-effective dose control. MATERIALS AND METHODS: This randomized double-blind, dosage-controlled trial was carried out in an outpatient clinic of traditional Chinese medicine in Hong Kong. Women with symptomatic uterine fibroids diagnosed according to the WHO International Classification of Diseases (ICD-10) were recruited and randomly assigned to one of two groups that received modified Guizhi Fuling Wan at either a low dose or the conventional dose on a daily basis for 16 weeks. This study was quality controlled by a data safety monitoring board. The primary outcome was the symptom severity as measured with the Uterine Fibroid Symptom-Quality of Life questionnaire. The secondary outcomes included quality of life, menstrual bleeding (measured on a pictorial blood loss assessment chart), pain severity (measured on the 6-point behavioral rating scale), change in Chinese medicine syndrome score, fibroid volume (measured by magnetic resonance imaging), hemoglobin level, and hormone levels. RESULTS: Seventy-eight women were recruited for this study. Between-groups comparison showed no significant difference at the endpoint for all outcomes except for the Chinese medicine syndrome score; however, at the endpoint, within-group comparison showed significant improvement in both groups relative to baseline in symptom severity, functional influence of pelvic pain, Chinese medicine syndrome score, and fibroid volume and uterus condition on magnetic resonance imaging (p < 0.05).The low-dose group yielded greater endpoint improvement in the Chinese medicine syndrome score than the conventional-dose group (p=0.024). No serious adverse events related to the intervention were noted. CONCLUSION: Both low-dose and conventional-dose preparations significantly ameliorated uterine fibroid-related symptoms and fibroid volume, although no significant difference was found between the low-dose and conventional-dose groups. The herbal formula GuizhiFuling Wan is safe in women with uterine fibroids.

3.
J Sci Food Agric ; 102(1): 214-222, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34061363

RESUMO

BACKGROUND: Fresh shiitake mushrooms are rich in nutrients, but have high water content, a fast metabolism after harvest, and deteriorate extremely easily. Therefore, the drying of shiitake mushrooms has become a research direction. However, the role of different drying techniques on shiitake mushroom quality is limited. Therefore, the purpose of this study was to investigate the effect of thermal and non-thermal drying on the drying kinetics, and the physicochemical properties of the end product. RESULTS: Results showed that shiitake mushroom treated with non-thermal drying (vacuum freeze-drying) had an attractive color, low shrinkage, and uniform honeycomb structure, while the drying time was the longest and not conducive to the formation of shiitake mushroom aroma. But shiitake mushroom treated with thermal drying presents an attractive fragrance. In thermal processing technology, compared with hot air convection drying (HAD), infrared hot air convection drying (IRHAD) shortens the drying time by 37.5%, and had the highest oxidation resistance, polysaccharide content and the lowest color change. Relative-humidity drying (RHD) samples had the lowest shrinkage compared with other thermal processing technology. The five polysaccharides exhibited similar preliminary structural characteristics, but the polysaccharides obtained by IRHAD have the highest antioxidant properties. CONCLUSION: These results showed that compared with thermal drying technology, non-thermal drying technology is not suitable for shiitake mushroom processing. In thermal processing technology, IRHAD is a potential drying method to obtain high-quality dried shiitake mushrooms and shiitake mushroom polysaccharide (SMP). However, it is necessary to increase the pretreatment technology to achieve the attractive appearance of non-thermal drying technology. © 2021 Society of Chemical Industry.


Assuntos
Dessecação/métodos , Conservação de Alimentos/métodos , Cogumelos Shiitake/química , Antioxidantes/química , Dessecação/instrumentação , Conservação de Alimentos/instrumentação , Temperatura Alta , Cinética , Água/química
4.
J Hazard Mater ; 423(Pt A): 127065, 2022 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-34523466

RESUMO

As carcinogenic and ubiquitous pollutants, an in-depth understanding of the long-term environmental behaviors of polycyclic aromatic hydrocarbons (PAHs) and their driving forces is crucial for reducing human health risks. Based on long-term monitoring data from 2001 to 2016, this study systematically investigated the temporal and seasonal trends, periodic oscillation, source apportionment, and human health risks of PAHs in eight rivers in the Free State of Saxony, Germany. The results showed that the annual average ∑16PAHs (sum of 16 PAH concentrations) ranged from 28.2 ng L-1 to 202 ng L-1. Using the Mann-Kendall test, a trend of decreasing PAH concentrations was determined (slope range: -0.103 to -0.0159). Wavelet analysis indicated that the most significant periodic oscillation of PAHs was 10-30 months, with more pollution in winter. Source apportionment analysis suggested that vehicular emissions and coal combustion contributed the most to PAH concentrations (20.6-40.3% and 21.7-41.4%, respectively) and related health risks (54.1-80.1% and 5.61-37.9%, respectively). Furthermore, the risks (oral lifetime: 4.24×10-7-1.34×10-6; dermal lifetime: 2.86×10-5-9.05×10-5) were determined to be low. The data revealed that the substitution of petroleum and coal with cleaner energy would facilitate the mitigation of PAHs.

5.
J Colloid Interface Sci ; 607(Pt 1): 760-768, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34534767

RESUMO

Poor visible light utilization and charge separation efficiency of TiO2 restrict its extensive application in the photocatalytic field. Herein, a specific Z-scheme TiO2-x/Cu/ZnO heterojunction was successfully constructed by atomic layer deposition (ALD) technique and spray pyrolysis technology. Benefited from the surface plasmon resonance (SPR) effect of Cu and Z-scheme heterojunction, the visible light absorption capacity was greatly enhanced. Meanwhile, ZnO nanolayer coating, prepared by ALD technique, protects Cu element to hinder its oxidation, thus enhancing the separation efficiency of photogenerated carriers. Therefore, the photocatalytic hydrogen production performance was significant improved, exhibiting a maximum value of 342.0 µmol·g-1·h-1 for the optimal B-T-0.1C-10Z (black TiO2/0.1Cu/10 nm ZnO) sample without any noble-metal cocatalyst, which is higher than pure TiO2 (310.7 µmol·g-1·h-1, with 3 wt% Pt) synthesized by spray pyrolysis method under equal conditions. In addition, a possible mechanism for the enhanced performance was briefly discussed based on the experimental results.

6.
Chemosphere ; 286(Pt 1): 131583, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34293558

RESUMO

Chemical degradation is one of the crucial methods for the remediation of hydrophobic organic compounds (HOCs) in soil/sediment. The sequestration effect of black carbon (BC) can affect the adsorption state of HOCs, thereby affecting their chemical degradability. Our study focused on the chemical degradability of 2-Chlorobiphenyl (PCB1) sequestrated on the typical BC (fly ash (FC), soot (SC), low-temperature biochar (BC400) and high-temperature biochar (BC900)) by iron-nickel bimetallic nanomaterials (nZVI/Ni) based on TENAX desorption technology. The results showed that PCB1 adsorbed in various states were simultaneously dechlorinated by nZVI/Ni. Specifically, rapid-desorption-state PCB1 tended to degrade more easily than resistant-desorption-state PCB1. Moreover, the degradation mechanism varied according to the type of BC. In the case of FC and SC, the degradation rate was lower than the desorption rate for the PCB1 in rapid and slow desorption states, and the degradation rate of PCB1 in the resistant desorption state was negligible. The PCB1 on FC and SC was first desorbed from BC and then degraded. However, in terms of BC400 and BC900, the degradation rate was higher than the desorption rate, and the degradation rate of the resistant-desorption-state PCB1 was 1.4 × 10-2 h-1 and 4.1 × 10-2 h-1, respectively. The graphitized structure of BC900 can directly transfer electrons, so more than 90% of the resistant-desorption-state PCB1 could be degraded. In addition, BC may affect the longevity of nZVI/Ni, thereby affecting its degradability. Therefore, the chemical degradability of BC-adsorbed HOCs should be comprehensively evaluated based on the adsorption state and the properties of BC.


Assuntos
Poluentes do Solo , Fuligem , Adsorção , Carvão Vegetal , Solo , Poluentes do Solo/análise , Tecnologia
7.
Front Plant Sci ; 12: 718981, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34721452

RESUMO

Light is highly heterogeneous in natural conditions, and plants need to evolve a series of strategies to acclimate the dynamic light since it is immobile. The present study aimed to elucidate the response of light reaction of photosynthesis to dynamic sunflecks in a shade-tolerant species Panax notoginseng and to examine the regulatory mechanisms involved in an adaptation to the simulated sunflecks. When P. notoginseng was exposed to the simulated sunflecks, non-photochemical quenching (NPQ) increased rapidly to the maximum value. Moreover, in response to the simulated sunflecks, there was a rapid increase in light-dependent heat dissipation quantum efficiency of photosystem II (PSII) (ΦNPQ), while the maximum quantum yield of PSII under light (F v'/F m') declined. The relatively high fluorescence and constitutive heat dissipation quantum efficiency of PSII (Φf,d) in the plants exposed to transient high light (400, 800, and 1,600 µmol m-2 s-1) was accompanied by the low effective photochemical quantum yield of PSII (ΦPSII) after the dark recovery for 15 min, whereas the plants exposed to transient low light (50 µmol m-2 s-1) has been shown to lead to significant elevation in ΦPSII after darkness recovery. Furthermore, PSII fluorescence and constitutive heat dissipation electron transfer rate (J f,d) was increased with the intensity of the simulated sunflecks, the residual absorbed energy used for the non-net carboxylative processes (J NC) was decreased when the response of electron transfer rate of NPQ pathway of PSII (J NPQ) to transient low light is restricted. In addition, the acceptor-side limitation of PSI [Y(NA)] was increased, while the donor-side limitation of photosystems I (PSI) [Y(ND)] was decreased at transient high light conditions accompanied with active cyclic electron flow (CEF). Meanwhile, when the leaves were exposed to transient high light, the xanthophyll cycle (V cycle) was activated and subsequently, the J NPQ began to increase. The de-epoxidation state [(Z + A)/(V + A + Z)] was strongly correlated with NPQ in response to the sunflecks. In the present study, a rapid engagement of lutein epoxide (Lx) after the low intensity of sunfleck together with the lower NPQ contributed to an elevation in the maximum photochemical quantum efficiency of PSII under the light. The analysis based on the correlation between the CEF and electron flow devoted to Ribulose-1, 5-bisphosphate (RuBP) oxygenation (J O) indicated that at a high light intensity of sunflecks, the electron flow largely devoted to RuBP oxygenation would contribute to the operation of the CEF. Overall, photorespiration plays an important role in regulating the CEF of the shade-tolerant species, such as P. notoginseng in response to transient high light, whereas active Lx cycle together with the decelerated NPQ may be an effective mechanism of elevating the maximum photochemical quantum efficiency of PSII under light exposure to transient low light.

8.
Zhongguo Dang Dai Er Ke Za Zhi ; 23(10): 981-986, 2021 Oct 15.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-34719411

RESUMO

OBJECTIVES: To study the safety and efficacy of dexmedetomidine hydrochloride combined with midazolam in fiberoptic bronchoscopy in children. METHODS: A total of 118 children who planned to undergo fiberoptic bronchoscopy from September 2018 to February 2021 were enrolled. They were divided into a control group (n=60) and an observation group (n=58) using a random number table. The observation group received intravenous pumping of dexmedetomidine hydrochloride (2 µg/mL) at 1 µg/kg and then intravenous injection of midazolam at 0.05 mg/kg, followed by dexmedetomidine hydrochloride pumped intravenously at 0.5-0.7 µg/(kg·h) 10 minutes later to maintain anesthesia. The control group was given intravenous pumping of propofol at 2 mg/kg and then intravenous injection of midazolam at 0.05 mg/kg, followed by propofol pumped intravenously at 4-6 mg/(kg·h) 10 minutes later to maintain anesthesia. Fiberoptic bronchoscopy was performed after the children were unconscious. Heart rate (HR), respiratory rate, blood oxygen saturation, and mean arterial pressure (MAP) were recorded before inserting the bronchoscope (T0), at the time of inserting the bronchoscope (T1), when the bronchoscope reached the glottis (T2), when the bronchoscope reached the carina (T3), and when the bronchoscope entered the bronchus (T4). The intraoperative peak airway pressure (Ppeak), examination time, degree of sedation, extent of amnesia, incidence of adverse reactions, postoperative awakening time, and postoperative agitation score were also recorded. RESULTS: Compared with the control group, the observation group had significantly decreased MAP at T1 to T4 and HR at T1 to T3 (P<0.05). Compared with that at T0, MAP was significantly increased at T1 to T4 in the control group and at T3 in the observation group (P<0.05). HR was significantly higher at T1 to T3 than at T0 (P<0.05). Compared with the control group, the observation group showed significantly lower intraoperative Ppeak value, incidence of intraoperative adverse reactions, and postoperative agitation score, significantly shorter examination time, and better effects of amnesia and anesthesia (P<0.05). There was no significant difference in the degree of intraoperative sedation and postoperative awakening time between the two groups (P>0.05). CONCLUSIONS: Dexmedetomidine hydrochloride combined with midazolam is a safe and effective way to administer general anesthesia for fiberoptic bronchoscopy in children, which can ensure stable vital signs during examination, reduce intraoperative adverse reactions and postoperative agitation, shorten examination time, and increase amnesic effect.


Assuntos
Dexmedetomidina , Midazolam , Brônquios , Broncoscopia , Criança , Dexmedetomidina/efeitos adversos , Humanos , Hipnóticos e Sedativos/efeitos adversos , Estudos Prospectivos
9.
Int J Mol Sci ; 22(22)2021 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-34830436

RESUMO

Laccase (LAC) plays important roles in different plant development and defense processes. In this study, we identified laccase genes (CsLACs) in Camellia sinensis cv 'Longjing43' cultivars, which were classified into six subclades. The expression patterns of CsLACs displayed significant spatiotemporal variations across different tissues and developmental stages. Most members in subclades II, IV and subclade I exhibited contrasting expression patterns during leaf development, consistent with a trade-off model for preferential expression in the early and late developmental stages. The extensive transcriptional changes of CsLACs under different phytohormone and herbivore treatment were observed and compared, with the expression of most genes in subclades I, II and III being downregulated but genes in subclades IV, V and VI being upregulated, suggesting a growth and defense trade-off model between these subclades. Taken together, our research reveal that CsLACs mediate multi-perspective trade-offs during tea plant development and defense processes and are involved in herbivore resistance in tea plants. More in-depth research of CsLACs upstream regulation and downstream targets mediating herbivore defense should be conducted in the future.

10.
Mol Ther Oncolytics ; 23: 355-366, 2021 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-34820505

RESUMO

Based on our initial bioinformatics finding of the upregulated expression of sentrin-specific protease 1 (SENP1) and cyclin E1 (CCNE1) in Wilms tumor, this study aimed to illustrate the molecular mechanism of SENP1 in Wilms tumor, which involved the hypoxia-inducible factor 1α (HIF-1α)/stanniocalcin-1 (STC1)/CCNE1 axis. Wilms tumor and adjacent normal tissues were clinically collected. Gain- and loss-of-function assays were performed to evaluate the effects of the regulatory axis on malignant phenotypes of Wilms tumor cells. A mouse model of Wilms tumor xenografts was further established for in vivo substantiation. Overexpression of CCNE1 and SENP1 occurred in Wilms tumor tissues and cells. Silencing SENP1 inhibited viability and enhanced cell-cycle arrest of Wilms tumor cells. SENP1 promoted STC1 expression and upregulated CCNE1 by driving the small ubiquitin-like modifier (SUMO)ylation of HIF-1α, which ultimately promoted the malignant phenotypes of Wilms tumor cells. It was further confirmed that silencing SENP1 downregulated the expression of CCNE1 and restricted tumorigenicity of Wilms tumor cells in vivo. Taken together, SENP1 elevated STC1 expression by driving the SUMOylation of HIF-1α, thereby upregulating the expression of CCNE1 and ultimately promoting the development of Wilms tumor.

12.
Environ Res ; : 112401, 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34801544

RESUMO

Oases environments in oases to be sensitive to anthropogenic activity because of ecological fragility. Polycyclic aromatic hydrocarbon (PAH) pollution resulting from anthropogenic activity leads to ecological degradation in oases. To examine the impact of anthropogenic activity on the oasis ecological environment, the present study focused on the spatial distribution and source apportionment of soil PAHs and bacterial community responses in typical oases in Xinjiang, China. The results showed that the soil PAH level were higher in the city centres of Urumqi (9-6340 µg kg-1), Aksu (8-957 µg kg-1) and Korla (8-1103 µg kg-1) and lower in the centres of Hotan city (11-268 µg kg-1) and Qira county (7-163 µg kg-1). Source apportionment suggested that gasoline emissions, diesel emissions, vehicle emissions, coal combustion, coke processing and biomass burning were the sources of soil PAHs. The integrated lifetime cancer risks of soil PAH exceeding the guideline safety values (10-6) recommended by United States Environmental Protection Agency. The ingestion and dermal exposure pathways caused the greatest health risk (contribution ≤82%). Additionally, in the soil with low PAH concentrations, the richness and evenness of the soil bacterial community were great, and the molecular ecological network (MEN) structure was complex. Among populations, Proteobacteria and Actinobacteria (relative abundance ≥17%) are the main dominant species in the bacterial communities and the keystone species in the MEN.

13.
Artigo em Inglês | MEDLINE | ID: mdl-34813574

RESUMO

ABSTRACT: Sodium-glucose cotransporter 2 (SGLT2) inhibitors have well-documented effects on reducing hospitalization for heart failure and cardiovascular mortality, while the effect on atrial fibrillation has not been comprehensively investigated. Therefore, we performed a meta-analysis to assess the association between SGLT2 inhibitors and atrial fibrillation risk by systematically searching PubMed, Embase and ClinicalTrials.gov. Two investigators independently identified randomized controlled trials which compared SGLT2 inhibitors with control in patients with type 2 diabetes, heart failure or chronic kidney disease. Primary outcomes were incident atrial fibrillation and stroke. We included 20 randomized trials involving 63604 patients. The SGLT2 inhibitors used were dapagliflozin (7 studies, 28834 patients), canagliflozin (7 studies, 17440 patients), empagliflozin (5 studies, 9082 patients) and ertugliflozin (1 study, 8246 patients). Follow-up ranged from 24 weeks to 202weeks. SGLT2 inhibitors treatment was associated with a significant attenuation in the risk of incident atrial fibrillation (OR=0.82, 95%CI:0.72-0.93, P=0.002) compared with control. No significant difference in stroke between SGLT2 inhibitors and control groups was found (OR=0.99, 95%CI:0.85-1.15, P=0.908). This present meta-analysis indicates that SGLT2 inhibitors are associated with a lower risk of incident atrial fibrillation and don't significantly affect stroke risk for patients with and without type 2 diabetes.

14.
Nanotechnology ; 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34814121

RESUMO

The gallium nitride (GaN) nanowires (NWs) in piezotronic applications are usually under cyclic loading, which thus may inevitably suffer the mechanical fatigue. In this paper, the fatigue behaviours of defective GaN NWs are investigated by using molecular dynamics (MD) simulations. Our results show no significant changes in the molecular structures of GaN NWs until their final failure during the fatigue process. The final fracture occurring in the GaN NWs under fatigue loading is triggered by the crack that unusually initiates from the NW surface. The GaN NW with a smaller defect concentration or under the fatigue load with a smaller amplitude is found to possess a longer fatigue life. In addition, the ultimate fatigue strain of GaN NWs can be significantly increased by reducing the defect concentration of NWs. The material parameters including elastic constants, piezoelectric coefficients, and dielectric constants of GaN NWs in the fatigue test are evaluated through MD simulations, all of which are found to keep almost unchanged during the fatigue process. These material parameters together with the band gaps of GaN NWs extracted from first-principles calculations are employed in finite element calculations to investigate the piezopotential properties of GaN NWs under fatigue loading. No significant changes are found in the piezopotential properties of GaN NWs during the fatigue process, which indicates the long-term dynamic reliability of GaN NWs in piezotronic applications.

15.
Integr Cancer Ther ; 20: 15347354211058695, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34802291

RESUMO

OBJECTIVE: Insomnia is a highly prevalent and disturbing symptom in breast cancer patients under or post chemotherapy. If not appropriately treated, it can persist for years after the completion of cancer treatments. Acupuncture has been widely used for alleviating insomnia. The aim of this study is to examine the feasibility, efficacy and safety of acupuncture for chemotherapy-related insomnia among patients with breast cancer. MATERIALS AND METHODS: This is a trial protocol for a randomized, sham-controlled, subject- and assessor-blinded clinical trial. A total of 138 eligible participants will be assigned randomly to acupuncture or sham control group at a ratio of 1:1. Participants in acupuncture group will receive electroacupuncture (EA) plus auricular acupressure (AA) treatment, while subjects in sham acupuncture group will receive sham EA plus sham AA. Both acupuncture and sham treatments will be given twice weekly for 6 weeks, followed by maintenance treatments once every 4 weeks for 12 weeks (15 sessions totally). The primary outcome is the change of Insomnia Severity Index score between baseline and the end of 6-week treatment. Secondary outcome measurements include Actiwatch, sleep diary, Pittsburgh Sleep Quality Index, Functional Assessment of Cancer Therapy-Breast Cancer, Hospital Anxiety and Depression Scale, Brief Pain Inventory-Short Form, Brief Fatigue Inventory, Acupuncture Expectancy Scale, credibility, and adverse events. Participants will be followed up to 42 weeks. CONCLUSIONS: This trial will expand our understanding of the feasibility, efficacy, and safety of acupuncture as a treatment for alleviating chemotherapy-related insomnia in patients with breast cancer. EA plus AA, if proven to be effective, can be implemented into routine settings to play a role in insomnia management for patients with breast cancer.

16.
Chem Commun (Camb) ; 57(92): 12270-12272, 2021 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-34751285

RESUMO

An automated Cas12a-microfluidic system was constructed to distinguish the B.1.617.2 (delta) variant of SARS-CoV-2 from the wild-type virus rapidly and was validated using 30 clinical samples, showing 100% consistency with next-generation sequencing. It will be a potential tool for the rapid differential diagnosis of the delta variant of SARS-CoV-2.


Assuntos
COVID-19/diagnóstico , Sistemas CRISPR-Cas/genética , Microfluídica/métodos , SARS-CoV-2/genética , Automação , COVID-19/virologia , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Mutação , Reação em Cadeia da Polimerase , RNA Guia/genética , RNA Guia/metabolismo , RNA Viral/metabolismo , SARS-CoV-2/isolamento & purificação
17.
Food Chem ; : 131574, 2021 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-34802801

RESUMO

The effect and mechanism of micro-/nano-scaled (MCB/NCB) chicken bones on improved gel properties of low-salt (0.5%) pork batters (LSPB) were investigated. Results showed that the Ca2+ released from MCB/NCB induced protein conformational transition of LSPB from α-helix and ß-turn to random coil, which facilitated the orderly protein aggregation, resulting in the formation of denser and more uniform gel network. The better-organized gel network not only improved the textural properties and gel strength, but also contributed to the cooking loss reduction, water release decline, water state transition from free water into more immobilized water, and proton density increase, resulting in better sensory attributes. NCB showed more preferable effects on the property improvement of LSPB gels than MCB, because of its smaller particle size, less redness and yellowness, higher dispersibility, and higher Ca2+ release. This study could help promote the high-value utilization of poultry by-product bones and development of low-salt meat products.

18.
ACS Synth Biol ; 2021 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-34762415

RESUMO

The oleaginous yeast Yarrowia lipolytica represents an environmentally friendly platform cell factory for ß-carotene production. However, Y. lipolytica is a dimorphic species that can undergo a yeast-to-mycelium transition when exposed to stress. The mycelial form is unfavorable for industrial fermentation. In this study, ß-carotene-producing Y. lipolytica strains were constructed via the integration of multiple copies of 13 genes related to the ß-carotene biosynthesis pathway. The ß-carotene content increased by 11.7-fold compared with the start strain T1. As the ß-carotene content increased, the oval-shaped yeast form was gradually replaced by hyphae, implying that the accumulation of ß-carotene in Y. lipolytica induces a morphological transition. To relieve this metabolic stress, the strains were morphologically engineered by deleting CLA4 and MHY1 genes to convert the mycelium back to the yeast form, which further increased the ß-carotene production by 139%. In fed-batch fermentation, the engineered strain produced 7.6 g/L and 159 mg/g DCW ß-carotene, which is the highest titer and content reported to date. The morphological engineering strategy developed here may be useful for enhancing chemical synthesis in dimorphic yeasts.

19.
BMC Gastroenterol ; 21(1): 430, 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34794384

RESUMO

BACKGROUND: Esophageal cancer (EC) is a common and lethal carcinoma; however, the effectiveness and feasibility of the chemo- and radio-therapy (CRT) for the elderly patients (≥ 70 years) with surgery have not been fully discussed. The purpose of this study was to investigate the potential effect of CRT on the prognosis. METHODS: A total of 1085 patients (534 CRT patients vs. 551 non-CRT patients) from 1998 to 2016 were collected from the Surveillance, Epidemiology, and End Results database according to the inclusion and exclusion criteria. Using the competing risk regression and survival analysis, an overall estimation of the effectiveness of CRT was performed on a well-balanced cohort via performing propensity score matching. Then, the specific impact of CRT on high- (n = 557) and low-risk (n = 528) cohorts derived from the nomogram's risk quantification for every patient were further evaluated respectively. Additionally, the advantages of the nomogram model and the conventional tumor, node, metastasis (TNM, 6th revision) staging system were compared. RESULTS: A better survival outcome was observed among patients receiving both surgery and CRT than those who underwent surgery alone (HR: 0.55, 95% CI 0.45-0.68, P < 0.001), especially for those with tumors characterized by poor differentiation, large tumor size, advanced T staging, lymphatic metastasis, and distant metastasis (HR: 0.48, 95% CI 0.39-0.59, P < 0.001), while no benefit was observed among the low-risk patients. Furthermore, the newly established nomogram model might be better than the TNM (6th revision) staging system but more data needed. CONCLUSION: Aggressive treatments, such as surgery, chemotherapy, and radiotherapy, were considered effective for selected elderly patients with EC according to the newly established nomogram model.


Assuntos
Neoplasias Esofágicas , Idoso , Neoplasias Esofágicas/terapia , Humanos , Metástase Linfática , Estadiamento de Neoplasias , Nomogramas , Prognóstico , Programa de SEER
20.
Sci Rep ; 11(1): 22481, 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34795364

RESUMO

We have performed sound velocity and unit cell volume measurements of three synthetic, ultrafine micro/nanocrystalline grossular samples up to 50 GPa using Brillouin spectroscopy and synchrotron X-ray diffraction. The samples are characterized by average grain sizes of 90 nm, 93 nm and 179 nm (hereinafter referred to as samples Gr90, Gr93, and Gr179, respectively). The experimentally determined sound velocities and elastic properties of Gr179 sample are comparable with previous measurements, but slightly higher than those of Gr90 and Gr93 under ambient conditions. However, the differences diminish with increasing pressure, and the velocity crossover eventually takes place at approximately 20-30 GPa. The X-ray diffraction peaks of the ultrafine micro/nanocrystalline grossular samples significantly broaden between 15-40 GPa, especially for Gr179. The velocity or elasticity crossover observed at pressures over 30 GPa might be explained by different grain size reduction and/or inhomogeneous strain within the individual grains for the three grossular samples, which is supported by both the pressure-induced peak broadening observed in the X-ray diffraction experiments and transmission electron microscopy observations. The elastic behavior of ultrafine micro/nanocrystalline silicates, in this case, grossular, is both grain size and pressure dependent.

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