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1.
Int J Biol Macromol ; 172: 178-185, 2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33421471

RESUMO

Tension wood is a type of defect of wood, however, it has some especial character and structure. In this study, cellulase lignin structures in normal and tension wood of Poplar 107 (Populus × euramericana cv. '74/76') were compared using ultraviolet (UV), Fourier transform infrared (FT-IR), Raman and magnetic resonance (NMR) spectroscopy, gel permeation chromatography (GPC) and elemental analysis. The results showed that the lignins in both normal and tension wood were dominated by syringyl units, followed by guaiacyl and p-hydroxyphenyl units. The FT-IR result presented that the relative intensity of syringyl units in normal and tension wood were 0.87 and 0.90. The contents of aliphatic hydroxyl, methoxyl, condensed phenolic hydroxyl and carboxyl of lignin in tension wood were higher than those of phenolic hydroxyl groups and ß-5. The contents of ß-O-4, ß-ß and ß-1 in tension wood were similar with those in normal wood. The average per-C9-unit formulae of the lignin in normal and tension wood of Poplar 107 were C9H7.21O1.79 (OH)1.17_x0001_(OCH3)1.55 and C9H7.23O1.76(OH)1.25_x0001_(OCH3)1.62, respectively.

2.
Sci Rep ; 11(1): 565, 2021 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-33436852

RESUMO

Increasing evidence suggests that circular RNAs (circRNAs) play critical roles in various pathophysiological activities. However, the role of circRNAs in inflammatory bowel disease (IBD) remains unclear. Here we report the potential roles of hsa_circRNA_103765 in regulating cell apoptosis induced by TNF-α in Crohn's disease (CD). We identify that CircRNA_103765 expression was significantly upregulated in peripheral blood mononuclear cells (PBMCs) of patients with active IBD. A positive correlation with TNF-α significantly enhanced circRNA_103765 expression in CD, which was significantly reversed by anti-TNF-α mAb (infliximab) treatment. In vitro experiments showed that TNF-α could induce the expression of circRNA_103765, which was cell apoptosis dependent, while silencing of circRNA_103765 could protect human intestinal epithelial cells (IECs) from TNF-α-induced apoptosis. In addition, circRNA_103765 acted as a molecular sponge to adsorb the miR-30 family and impair the negative regulation of Delta-like ligand 4 (DLL4). Collectively, CircRNA_103765 is a novel important regulator of the pathogenesis of IBD via sponging miR-30 family-mediated DLL4 expression changes. Blockade of circRNA_103765 could serve as a novel approach for the treatment of IBD patients.

3.
J Cell Physiol ; 2021 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-33452710

RESUMO

While mesenchymal stem cells (MSCs) have been widely used to repair radiation-induced bone damage, the molecular mechanism underlying the effects of MSCs in the maintenance of bone homeostasis under radiation stress remains largely unknown. In this study, the role and mechanisms of R-spondin 1 (Rspo1)-leucine-rich repeat-containing G protein-coupled receptor 4 (LGR4) axis on the initiation of self-defense of bone mesenchymal stem cells (BMSCs) and maintenance of bone homeostasis under radiation stress were investigated. Interestingly, radiation increased levels of Rspo1 and LGR4 in BMSCs. siRNA knockdown of Rspo1 or LGR4 aggravated radiation-induced impairment of self-renewal ability and osteogenic differentiation potential of BMSCs. However, exogenous Rspo1 significantly attenuated radiation-induced depletion of BMSCs, and promoted the lineage shift towards osteoblasts. This alteration was associated with the reversal of mammalian target of rapamycin (mTOR) activation and autophagy decrement. Pharmacological and genetic blockade of autophagy attenuated the radio-protective effects of Rspo1, rendering BMSCs more vulnerable to radiation-induced injury. Then bone radiation injury was induced in C57BL6J mice to further determine the radio-protective effects of Rspo1. In mice, administration of Rspo1 recombinant protein alleviated radiation-induced bone loss. Our results uncover that Rspo1-LGR4-mTOR-autophagy axis are key mechanisms by which BMSCs initiate self-defense against radiation and maintain bone homeostasis. Targeting Rspo1-LGR4 may provide a novel strategy for the intervention of radiation-induced bone damage.

4.
Cancer Res Treat ; 53(1): 261-269, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32972045

RESUMO

PURPOSE: We sought to investigate the effectiveness and safety of dabrafenib in children with BRAFV600E-mutated Langerhans cell histiocytosis (LCH). Materials and Methods: A retrospective analysis was performed on 20 children with BRAFV600E-mutated LCH who were treated with dabrafenib. RESULTS: The median age at which the patients started taking dabrafenib was 2.3 years old (range, 0.6 to 6.5 years). The ratio of boys to girls was 2.3:1. The median follow-up time was 30.8 months (range, 18.9 to 43.6 months). There were 14 patients (70%) in the risk organ (RO)+ group and six patients (30%) in the RO- group. All patients were initially treated with traditional chemotherapy and then shifted to targeted therapy due to poor control of LCH or intolerance to chemotherapy. The overall objective response rate and the overall disease control rate were 65% and 75%, respectively. During treatment, circulating levels of cell-free BRAFV600E (cfBRAFV600E) became negative in 60% of the patients within a median period of 3.0 months (range, 1.0 to 9.0 months). Grade 2 or 3 adverse effects occurred in five patients. CONCLUSION: Some children with BRAFV600E-mutated LCH may benefit from monotherapy with dabrafenib, especially high-risk patients with concomitant hemophagocytic lymphohistiocytosis and intolerance to chemotherapy. The safety of dabrafenib is notable. A prospective study with a larger sample size is required to determine the optimal dosage and treatment duration.

5.
Environ Sci Pollut Res Int ; 28(1): 473-481, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32815008

RESUMO

In recent 2 years, the incidence of influenza showed a slight upward trend in Guangxi; therefore, some joint actions should be done to help preventing and controlling this disease. The factors analysis of affecting influenza and early prediction of influenza incidence may help policy-making so as to take effective measures to prevent and control influenza. In this study, we used the cross correlation function (CCF) to analyze the effect of climate indicators on influenza incidence, ARIMA and ARIMAX (autoregressive integrated moving average model with exogenous input variables) model methods to do predictive analysis of influenza incidence. The results of CCF analysis showed that climate indicators (PM2.5, PM10, SO2, CO, NO2, O3, average temperature, maximum temperature, minimum temperature, average relative humidity, and sunshine duration) had significant effects on the incidence of influenza. People need to take good precautions in the days of severe air pollution and keep warm in cold weather to prevent influenza. We found that the ARIMAX (1,0,1)(0,0,1)12 with NO2 model has good predictive performance, which can be used to predict the influenza incidence in Guangxi, and the predicted incidence may be useful in developing early warning systems and providing important evidence for influenza control policy-making and public health intervention.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Influenza Humana , Poluentes Atmosféricos/análise , Poluição do Ar/análise , China/epidemiologia , Clima , Humanos , Incidência , Influenza Humana/epidemiologia
6.
Epilepsy Behav ; 114(Pt A): 107572, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33268015

RESUMO

OBJECTIVE: The goal of this study was to evaluate the predictive capacity of four scoring tools: the Status Epilepticus Severity Score (STESS), the Encephalitis-NCSE-Diazepam resistance-Image abnormalities-Tracheal intubation (END-IT) score, and two variable combinations of the Epidemiology-based Mortality Score in Status Epilepticus (EMSE) in younger and older adult patients with status epilepticus (SE). METHODS: We present a retrospective hospital-based analysis with a focus on adult patients with SE at three tertiary care hospitals in the Zhejiang province of China. Data were collected from January 2013 to December 2018. The patients were divided into two groups: younger adult patients (18-64 years old) and older adult patients (≥65 years old). Clinical outcomes (dead or alive) were assessed at hospital discharge. The four scoring tools were used to predict in-hospital mortality in both younger and older adult patients. RESULTS: The mortality rate in older adult patients (25.4%) was higher than in younger adult patients (12.9%). Compared with the elderly, the younger adult patients had a higher proportion of encephalitis, while acute cerebrovascular disease and Charlson Complications Index (CCI) were lower. For the younger adult patients, END-IT had the largest area under the curve (AUC) of 0.843 (95% CI, 0.772-0.899), which was higher than the EMSE-EAL value of 0.687 (95% CI, 0.603-0.763, p < 0.05) and EMSE-EAC of 0.646 (95% CI, 0.561-0.725, p < 0.05). For the older adult patients, EMSE-EAL had the largest AUC of 0.843 (95% CI, 0.738-0.919), which was significantly higher than STESS with an AUC of 0.676 (95% CI, 0.554-0.782, p < 0.05). Moreover, the AUC of EMSE-EAL in the elderly was larger than in younger adult patients. The cutoffs in younger adult patients were STESS ≥ 4 (sensitivity 0.444, specificity 0.951), END-IT ≥ 3 (sensitivity 0.833, specificity 0.672), EMSE-EAL ≥ 31 (sensitivity 0.778, specificity 0.566), and EMSE-EAC ≥ 33 (sensitivity 0.833, specificity 0.492). However, the cutoffs in older adult patients were STESS ≥ 5 (sensitivity 0.500, specificity 0.925), END-IT ≥ 2 (sensitivity 0.944, specificity 0.547), EMSE-EAL ≥ 30 (sensitivity 0.944, specificity 0.623), and EMSE-EAC ≥ 31 (sensitivity 0.944, specificity 0.415). CONCLUSION: Our results indicated that the STESS, END-IT, EMSE-EAC, and EMSE-EAL scores have excellent capacity to predict in-hospital mortality in both younger and older adult patients with SE. Our study supports the use of END-IT in patients under 65 years of age and suggests that EMSE-EAL is the most suitable scoring tool for patients over 65.

7.
J Colloid Interface Sci ; 586: 279-291, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33189319

RESUMO

The development of robust, stain-resistant, and self-healing liquid-repellent surfaces is a common aspiration of both consumer and industrial applications, but the existing methods suffer from limitations, such as complicated procedures, weak mechanical durability and substrate dependency. In this work, a lubricant-grafted slippery surface (LGSS) was prepared by grafting vinyl-terminated polydimethylsiloxane (Vi-PDMS) onto various substrates coated with sulfhydryl-modified hollow mesoporous silica (SH-HMS) through a thiol-ene click reaction. The uniform and intact lubricant layer can effectively decrease the absorption of the polysaccharide and protein, exhibiting superior antifouling properties. Notably, the hollow structure of SH-HMS could significantly increase the oil grafting capacity of the slippery surface from 0.013 g/cm2 to 0.027 g/cm2 compared with the surface constructed by solid silica. By virtue of the strong covalent bond forces between the lubricant oil and surfaces, the obtained LGSS exhibited robust liquid repellency when subjected to high/low temperature, ultraviolet irradiation and water impact. Moreover, the liquid-repellent LGSS exhibited good self-repairing performance owing to the directional migration of the Vi-PDMS chain segment from the hollow capsule to the surface through the mesoporous channels under heating treatment. Therefore, such a newly developed strategy for constructing liquid-repellent coatings on various substrates with self-repairing properties has the potential to promote the advancement of interfacial antifouling materials and exhibit tremendous potential for consumer and industrial applications.

8.
J Hazard Mater ; 403: 123922, 2021 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-33264973

RESUMO

Arsenic is a toxic metalloid for both animals and plants. The signaling molecule melatonin can enhance abiotic stress tolerance, but the effects of As and melatonin on tea plants and the mechanisms of resilience remain unclear. Here we report that excess As causes severe oxidative stress in tea leaves as revealed by significantly reduced maximal photochemical efficiency of photosystem-II, and increased reactive oxygen species accumulation and lipid peroxidation. However, exogenous melatonin application alleviated the As phytotoxicity and increased the anthocyanin content upto 69.4 % by selectively upregulating the expression of its biosynthetic genes such as CsCHS and CsANS. Comparison of As tolerance between two tea genotypes differing in basal levels of anthocyanin revealed that a tea cultivar with increased anthocyanin content, Zijuan (ZJ), showed enhanced tolerance to As stress compared with Longjing 43 (LJ43) that contained relatively low levels of anthocyanin. Interestingly, exogenous anthocyanin also enhanced As tolerance in LJ43, but exogenous melatonin did not improve As tolerance in ZJ genotype. Analysis of As content in tea leaves revealed that melatonin significantly reduced As content in LJ43 but not in ZJ, suggesting that melatonin-enhanced tolerance to As stress is largely dependent on the basal levels of anthocyanin in tea plants.

9.
Sci Total Environ ; 754: 142069, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33254894

RESUMO

Agriculture has played an indispensable role in the economic and social development of China. However, the inappropriate application of fertilizers in agriculture has brought about environmental pollution. Therefore, under the requirements of green development, the inevitable choice has been reducing the application amount while increasing the utilization rate of fertilizers. To solve the problems resulting from fertilizers, this research designed and made Balanced Nutrient Fertilizer based on the nutrient balance method. To test Balanced Nutrient Fertilizer, continuous planting experiments on three crops (peas, corns, and peaches) were carried out in Pinggu District, Beijing. Furthermore, a substance flow analysis (SFA) was conducted to obtain a deeper understanding of the Balanced Nutrient Fertilizer. Through a series of tests, the application of Balanced Nutrient Fertilizer was shown to increase the crop yield by 3%. The product quality was also improved. In addition, the amount of nutrients applied was significantly reduced in the experimental group (specifically, 35%-88% for nitrogen, 69%-93% for phosphorus, and 8%-82% for potassium). The results of the SFA revealed that applying the Balanced Nutrient Fertilizer could meet the required amount of nutrients for the best crop growth, greatly decrease the negative influence of chemical fertilizers on the soil and reduce agricultural non-point source pollution.

10.
Mol Med Rep ; 23(1)2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33236150

RESUMO

Subsequently to the publication of the above article, the authors have realized that the bar charts shown for Fig. 3A and B, as they appeared in the paper, were the same as the bar charts shown for Fig. 4B and D. Fig. 3, as it should have appeared, is shown below. All the authors agree to this Corrigendum. Note that the revisions made to this figure do not adversely affect the results reported in the paper, or the conclusions stated therein. The authors regret that the duplication of the histograms in Fig. 4 as Fig. 3 was not noticed prior to the publication of this article, and offer their apologies to the Editor of Molecular Medicine Reports and to the readers of the Journal. [the original article was published in Molecular Medicine Reports 22: 4611-4618, 2020; DOI: 10.3892/mmr.2020.11564].

11.
Nanomaterials (Basel) ; 10(12)2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-33255592

RESUMO

Tin disulfide (SnS2) is regarded as one of the most suitable candidates as the electrode material for sodium-ion batteries (SIBs). However, the easy restacking and volume expansion properties of SnS2 during the charge/discharge process lead to the destruction of the electrode structure and a decrease in capacity. We successfully synthesized a SnS2 nanocrystalline-anchored three-dimensional porous graphene composite (SnS2/3DG) by combining hydrothermal and high-temperature reduction methods. The SnS2 nanocrystalline was uniformly dispersed within the connected reduced graphene oxide matrix. The SnS2/3DG battery showed a high reversible capacity of 430 mAh/g after 50 cycles at 100 mA/g. The SnS2/3DG composite showed an excellent rate capability with the current density increasing from 100 mA/g to 2 A/g. The excellent performance of the novel SnS2/3DG composite is attributed to the porous structure, which not only promoted the infiltration of electrolytes and hindered volume expansion for the porous structure, but also improved the conductivity of the whole electrode, demonstrating that the SnS2/3DG composite is a prospective anode for the next generation of sodium-ion batteries.

12.
Onco Targets Ther ; 13: 12357-12363, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33293828

RESUMO

Langerhans cell histiocytosis (LCH) is a rare myeloid neoplasia in children. BRAF mutations are associated with permanent diabetes insipidus (DI). The onset of clinical DI is always latent with imaging evidence of pituitary involvement. In this study, we reported three children with BRAF-mutated LCH with pituitary involvement who improved after targeted therapy (dabrafenib and trametinib). The results may indicate that pituitary involvement may be reversible in some patients with LCH if it is observed and treated before clinical DI. Targeted therapy may be an effective choice for LCH patients with early pituitary involvement who were irresponsive to first-line or second-line chemotherapy. However, a relapse after targeted therapy is completed in patients with persistently positive cfBRAF V600E mutations is still a challenge that needs to be resolved. The timing of introducing targeted therapy, its appropriate duration and effective combinations with chemotherapy or other targeted drugs should be explored further.

13.
Medicine (Baltimore) ; 99(50): e23564, 2020 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-33327312

RESUMO

BACKGROUND: Peripheral neurotoxicity (PN) is a frequent side effect of oxaliplatin treatment, and also is its dose-limiting toxicity. Studies have confirmed that ω-3 polyunsaturated fatty acids (ω-3 PUFAs) had a neuroprotective effect. However, the efficacy of ω-3 PUFAs on the prevention of oxaliplatin-related neurotoxicity remains unclear. We assessed the effect of ω-3 PUFAs on the neurotoxicity in colon cancer patients treated by oxaliplatin combined with capecitabine. METHODS: In a randomized, double-blind, placebo-controlled study, 179 patients with colon cancer receiving oxaliplatin combined with capecitabine were recruited, and randomly assigned to take ω-3 PUFAs, 640 mg t.i.d during chemotherapy and 1 month after the end of the treatment or placebo. All patients were treated with chemotherapy for 6 treatment cycles. The incidence and severity of PN were evaluated, and the nerve conduction was measured before the onset of chemotherapy and 1 month after treatment. In addition, the quality of life was also accessed using Chinese version of European organization for research and treatment of cancer quality of life questionnaire. RESULTS: The incidence of PN in the ω-3 PUFAs group and placebo group was 52.22% and 69.66%, respectively (P = .017). In addition, there was a significant difference in the severity of PN between the 2 groups (P = .017). In terms of motor and sensory nerve conduction, the sensory action potentials amplitude of sural nerve in the ω-3 PUFAs group and placebo group after chemotherapy treatment were (15.01 ±â€Š3.14) and (13.00 ±â€Š3.63) µ V respectively, suggesting there was a significant difference in the 2 groups (P = .000). In addition, the mean score of the global health-status/quality of life was obviously higher in the ω-3 PUFAs group than that in the placebo group. CONCLUSION: ω-3 PUFAs seem to reduce the incidence and severity of oxaliplatin-related neurotoxicity, and improve the quality of patients' life, indicating it is expected to be a potential drug for the treatment of oxaliplatin-related neurotoxicity.


Assuntos
Capecitabina/uso terapêutico , Neoplasias do Colo/tratamento farmacológico , Ácidos Graxos Ômega-3/uso terapêutico , Fármacos Neuroprotetores/uso terapêutico , Síndromes Neurotóxicas/prevenção & controle , Oxaliplatina/toxicidade , Idoso , Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem , Protocolos de Quimioterapia Combinada Antineoplásica/toxicidade , Capecitabina/administração & dosagem , Método Duplo-Cego , Esquema de Medicação , Ácidos Graxos Ômega-3/administração & dosagem , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fármacos Neuroprotetores/administração & dosagem , Síndromes Neurotóxicas/etiologia , Oxaliplatina/administração & dosagem , Oxaliplatina/uso terapêutico , Doenças do Sistema Nervoso Periférico/induzido quimicamente , Doenças do Sistema Nervoso Periférico/prevenção & controle , Qualidade de Vida , Inquéritos e Questionários
14.
Nat Prod Res ; : 1-9, 2020 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-33375863

RESUMO

A new cyclo-heptadepsipeptide xanthostatin B (1), together with isobutyryl hexapeptide (2), xanthostatin (3), TXS-1 (4) and TXS-2 (5), were isolated from the marine sponge derived Streptomyces sp. SCSIO 40064. The structures were elucidated by comprehensive spectroscopic data analyses and comparison with the literatures. The D-Val unit in 1 was assigned by Marfey's method. The absolute configuration of 4 was determined by X-ray crystallographic analysis. Compounds 1‒5 were evaluated for the inhibitory activities against four pharmaceutical targets and six antibacterial indicator strains. Compound 5 displayed α-glucosidase inhibitory activity with IC50 value of 18.67 ± 1.27 µM.

15.
Biomed Res Int ; 2020: 8824737, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33344649

RESUMO

In clinical practice, skin defects occur frequently due to various kinds of acute and chronic diseases. The standard treatment for these wounds is autografting, which usually results in complications such as scar formation and new wounds at donor sites. The advent of dermal substitutes has provided a novel method for wound repair, and rapid angiogenesis of the dermal substitutes is crucial for the graft to take. At present, many strategies have been developed to improve the process of vascularisation, some of which have shown promising potentials, but they could be very far from clinical applications. Most recently, negative-pressure wound therapy (NPWT) has been used extensively in clinical practice for wound care and management. It has been reported that NPWT reduces the time required for vascular ingrowth into the dermal substitute and improves graft take, indicating great potentials for wound repair. This article presents a comprehensive overview of the combined use of NPWT and dermal substitutes for tissue repair and regeneration. Relative concerns and prospects are also discussed.

16.
Cell Death Dis ; 11(12): 1051, 2020 12 11.
Artigo em Inglês | MEDLINE | ID: mdl-33311443

RESUMO

Increasing lines of evidence indicate the role of long non-coding RNAs (LncRNAs) in gene regulation and tumor development. Hence, it is important to elucidate the mechanisms of LncRNAs underlying the proliferation, metastasis, and invasion of lung adenocarcinoma (LUAD). We employed microarrays to screen LncRNAs in LUAD tissues with and without lymph node metastasis and revealed their effects on LUAD. Among them, Linc00426 was selected for further exploration in its expression, the biological significance, and the underlying molecular mechanisms. Linc00426 exhibits ectopic expression in LUAD tissues and cells. The ectopic expression has been clinically linked to tumor size, lymphatic metastasis, and tumor differentiation of patients with LUAD. The deregulation of Linc00426 contributes to a notable impairment in proliferation, invasion, metastasis, and epithelial-mesenchymal transition (EMT) in vitro and in vivo. Mechanistically, the deregulation of Linc00426 could reduce cytoskeleton rearrangement and matrix metalloproteinase expression. Meanwhile, decreasing the level of Linc00426 or increasing miR-455-5p could down-regulate the level of UBE2V1. Thus, Linc00426 may act as a competing endogenous RNA (ceRNA) to abate miR-455-5p-dependent UBE2V1 reduction. We conclude that Linc00426 accelerates LUAD progression by acting as a molecular sponge to regulate miR-455-5p, and may be a potential novel tumor marker for LUAD.

17.
Artigo em Inglês | MEDLINE | ID: mdl-33303421

RESUMO

INTRODUCTION: The prognostic value of B-cell lymphoma 2 (BCL2) expression in de novo diffuse large B-cell lymphoma (DLBCL) treated with immunochemotherapy is of interest to define a target patient population for clinical development of BCL2 inhibitors. We aimed to develop a reproducible immunohistochemistry algorithm and assay to determine BCL2 protein expression and assess the prognostic value of BCL2 in newly diagnosed DLBCL cohorts. PATIENTS AND METHODS: The prospectively defined algorithm incorporated BCL2 staining intensity and percentage of BCL2-positive cells. Functionally relevant cutoffs were based on the sensitivity of lymphoma cell lines to venetoclax. This assay was highly reproducible across laboratories. The prognostic impact of BCL2 expression was assessed in DLBCL patients from the phase 3 MAIN (n = 230) and GOYA (n = 366) trials, and a population-based registry (n = 310). RESULTS: Approximately 50% of tumors were BCL2 positive, with a higher frequency in high International Prognostic Index (IPI) and activated B-cell-like DLBCL subgroups. BCL2 expression was associated with poorer progression-free survival in the MAIN study (hazard ratio [HR], 1.66; 95% confidence interval [CI], 0.81-3.40; multivariate Cox regression adjusted for IPI and cell of origin). This trend was confirmed in the GOYA and registry cohorts in adjusted multivariate analyses (GOYA: HR, 1.72; 95% CI, 1.05-2.82; registry: HR, 1.89; 95% CI, 1.29-2.78). Patients with BCL2 immunohistochemistry-positive and IPI-high disease had the poorest prognosis: 3-year progression-free survival rates were 51% (GOYA) and 37% (registry). CONCLUSION: Findings support use of our BCL2 immunohistochemistry scoring system and assay to select patients with BCL2-positive tumors for future studies.

18.
J Musculoskelet Neuronal Interact ; 20(4): 614-618, 2020 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-33265091

RESUMO

Wilson's disease (WD) is an autosomal recessive genetic disease linked to ATP7B, which is located on the chromosome 13q14.3. We presently report a hepatolenticular degeneration carrier whose clinical phenotype mainly included limb weakness and tremor with a novel WD mutation. The mutation in Exon 10 of ATP7B Gene [c.2480G>A p. (Arg827Gln)] was identified after gene sequencing. We have provided diagnostic analyses, such as muscle biopsy and electrophysiology, which would be helpful to deepen the understanding of the pathogenesis underneath nerve damage in WD heterozygote carriers (Hzc).

19.
Environ Pollut ; : 116040, 2020 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-33280913

RESUMO

Biochar has the potential to remediate heavy metals in agricultural soil and mitigate nitrous oxide (N2O) emissions; however, the effects of biochar on heavy metal remediation, the soil microbial community and N2O emissions are not completely understood. In this study, we conducted a pot experiment in which Glycine max L. (soybean) was cultivated in two cadmium (Cd)-contaminated soils (low, 3.14 mg kg-1; high, 10.80 mg kg-1) to investigate the effects of biochar on the bioremediation of Cd, N2O emissions and the rhizosphere microbial community structure. The bioaccumulation of Cd in the plant shoots and roots increased with all biochar addition rates (0%, 1%, 5% and 10%); unexpectedly, the translocation capacity of Cd to the edible parts of the plant significantly decreased to 0.58 mg kg-1, which was close to the edible threshold (0.4 mg kg-1). The abundance and activities of functional marker genes of microbial nitrification (amoA) and denitrification (nirK, nirS and nosZ) were quantified with quantitative PCR, and we found that biochar addition reduced the precursor production of rhizoshpere N2O by inhibiting the transcription of the nirK gene. In addition, the nitrogenase activity during anthesis (S) was significantly (P < 0.05) increased with 1% (v/v) biochar addition. Noticeably, biochar addition only changed the microbial community structure in the very first stage before eventually stabilize. This study highlighted that biochar has the potential ability to maintain the quality of agricultural crops, remediate Cd-contaminated soils and may help reduce N2O emissions without disturbing the microbial community.

20.
Ying Yong Sheng Tai Xue Bao ; 31(11): 3823-3832, 2020 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-33300733

RESUMO

The ecological climate suitability of Boletus edulis was systematically analyzed in mountainous areas of the Western Sichuan Plateau using DEM, land cover data and meteorological data from 52 weather stations in Sichuan Province and four weather stations in other provinces. Factors such as temperature, precipitation and vegetation cover were selected as the regionalization index to analyze the potential spatial distribution of B. edulis in Western Sichuan Plateau by GIS technology. The results showed that the northern boundary of potential distribution area for B. edulis was near 32° N, the upper and lower limits of the altitude were about 800 m and 3000 m. The total distribution area was 2863000 hm2, accounting for about 9.7% of the whole study area. The Panxi area at south of 29° N was the main distribution area, accounting for about 90% of all potential distribution areas. Among the main distribution area, the suitable area was about 20%, and the remaining is the secondary suitable area. The suitable area was mainly distributed in the Anning River basin at the elevation of 1000 m to 2600 m in East of the Yalong River in the Panxi area. The secondary suitable area was mainly located in forest area extending upward and downward from suitable area at an altitude of about 3000 m and 800 m, respectively. The unsuitability areas were the alpine regions at altitude above 3000 m and the dry-hot valley regions at altitude below 800 m.


Assuntos
Altitude , Clima , Basidiomycota , China , Temperatura
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