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1.
Bioact Mater ; 19: 418-428, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35574059

RESUMO

Labeling of mesenchymal stem cells (MSCs) with superparamagnetic iron oxide nanoparticles (SPIONs) has emerged as a potential method for magnetic resonance imaging (MRI) tracking of transplanted cells in tissue repair studies and clinical trials. Labeling of MSCs using clinically approved SPIONs (ferumoxytol) requires the use of transfection reagents or magnetic field, which largely limits their clinical application. To overcome this obstacle, we established a novel and highly effective method for magnetic labeling of MSC spheroids using ferumoxytol. Unlike conventional methods, ferumoxytol labeling was done in the formation of a mechanically tunable biomimetic hydrogel-induced MSC spheroids. Moreover, the labeled MSC spheroids exhibited strong MRI T2 signals and good biosafety. Strikingly, the encapsulated ferumoxytol was localized in the extracellular matrix (ECM) of the spheroids instead of the cytoplasm, minimizing the cytotoxicity of ferumoxytol and maintaining the viability and stemness properties of biomimetic hydrogel-induced MSC spheroids. This demonstrates the potential of this method for post-transplantation MRI tracking in the clinic.

2.
Environ Pollut ; 316(Pt 2): 120597, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36343856

RESUMO

Sand and dust have significant impacts on air quality, climate, and human health. To investigate the influences of dust storms on chemical characterization and source contributions of fine particulate matter (PM2.5) in areas with different distances from dust source regions, PM2.5 and associated chemical composition were measured in two industrial cities with one near sand sources (i.e., Wuhai) and the other far from sand sources (i.e., Jinan) in northern China in March 2021. Results showed that PM mass concentrations significantly increased and exceeded the Chinese National Ambient Air Quality standard during the dust events, with absolute concentrations and fractional contributions of PM2.5-bound crustal and trace elements increased while secondary inorganic ions decreased at both sites. Crustal materials dominated the increased PM2.5 mass from non-dust period to dust period in both cities. These were further evidenced by PM2.5 source apportionment results from positive matrix factorization model. During the dust events, dust sources contributed up to 88% of PM2.5 mass in Wuhai and ∼38% of PM2.5 mass in Jinan, a city about thousands of kilometers away from the sand source. Besides, the measurement data indicated that dust from northwest China may also bring along with high abundance of organic matter and vanadium. Secondary and traffic sources were two of the most important source contributors to PM2.5 in both cities during the non-dust periods. However, the near sand source city was more susceptible to the aggravating effects of dust and minerals, with much higher contributions by crustal materials (∼47%, from the aspect of chemical components) and dust-related sources (∼26%, from the aspect of sources) to PM2.5 mass even during non-dust periods. This study highlighted the urgent need for more action and effective control of sand sources to reduce the impact on air quality in downstream regions.


Assuntos
Poluentes Atmosféricos , Material Particulado , Humanos , Material Particulado/análise , Poeira/análise , Poluentes Atmosféricos/análise , Areia , Monitoramento Ambiental/métodos , China , Emissões de Veículos/análise , Estações do Ano
3.
Ophthalmol Sci ; 3(1): 100222, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36325476

RESUMO

Purpose: Two novel deep learning methods using a convolutional neural network (CNN) and a recurrent neural network (RNN) have recently been developed to forecast future visual fields (VFs). Although the original evaluations of these models focused on overall accuracy, it was not assessed whether they can accurately identify patients with progressive glaucomatous vision loss to aid clinicians in preventing further decline. We evaluated these 2 prediction models for potential biases in overestimating or underestimating VF changes over time. Design: Retrospective observational cohort study. Participants: All available and reliable Swedish Interactive Thresholding Algorithm Standard 24-2 VFs from Massachusetts Eye and Ear Glaucoma Service collected between 1999 and 2020 were extracted. Because of the methods' respective needs, the CNN data set included 54 373 samples from 7472 patients, and the RNN data set included 24 430 samples from 1809 patients. Methods: The CNN and RNN methods were reimplemented. A fivefold cross-validation procedure was performed on each model, and pointwise mean absolute error (PMAE) was used to measure prediction accuracy. Test data were stratified into categories based on the severity of VF progression to investigate the models' performances on predicting worsening cases. The models were additionally compared with a no-change model that uses the baseline VF (for the CNN) and the last-observed VF (for the RNN) for its prediction. Main Outcome Measures: PMAE in predictions. Results: The overall PMAE 95% confidence intervals were 2.21 to 2.24 decibels (dB) for the CNN and 2.56 to 2.61 dB for the RNN, which were close to the original studies' reported values. However, both models exhibited large errors in identifying patients with worsening VFs and often failed to outperform the no-change model. Pointwise mean absolute error values were higher in patients with greater changes in mean sensitivity (for the CNN) and mean total deviation (for the RNN) between baseline and follow-up VFs. Conclusions: Although our evaluation confirms the low overall PMAEs reported in the original studies, our findings also reveal that both models severely underpredict worsening of VF loss. Because the accurate detection and projection of glaucomatous VF decline is crucial in ophthalmic clinical practice, we recommend that this consideration is explicitly taken into account when developing and evaluating future deep learning models.

4.
Synth Syst Biotechnol ; 8(1): 33-45, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36381963

RESUMO

The current petroleum chemical methods for fumaric acid production can cause heavy pollution and global warming. In this study, the engineered strains of A. pullulans var. aubasidani were found to be suitable for green fumaric acid producer. Removal and complementation of the relevant genes showed only the ornithine-urea cycle (OUC) was involved in high level fumarate biosynthesis which was controlled by the Ca2+ signaling pathway. Removal of both the GOX gene encoding glucose oxidase and the PKS1 gene encoding the polyketide synthase for 3,5-dihydroxydecanoic acid biosynthesis and overexpression of the PYC gene encoding pyruvate carboxylase made the strain e-PYC produce 88.1 ± 4.3 g/L of fumarate at flask level and 93.9 ± 0.8 g/L of fumarate during the fed-batch fermentation. As a yeast-like fungal strain, it was very easy to cultivate A. pullulans var. aubasidani DH177 and their mutants in the bioreactor and to edit its genomic DNAs to enhance fumarate production. It was found that 2 mol of CO2 could be fixed during a maximal theoretical yield of 2 mol of fumarate per mole of glucose consumed in the OUC. Therefore, the OUC-mediated fumarate biosynthesis pathway in A. pullulans var. aubasidani was a green and eco-friendly process for the global sustainable development and carbon neutrality.

5.
Sci Total Environ ; 857(Pt 3): 159658, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36302440

RESUMO

The combined process of flocculation and filtration can improve algae harvesting performance by combining the benefits of both and overcoming the drawbacks. The entire process was thoroughly examined in this study, considering technical and economic feasibility under a variety of operating situations. Dead-end filtration was performed to evaluate the harvesting performance, the removal of extracellular organic matter and the changes of flocs. Cross-flow filtration was then carried out to explore the effect of operating parameters on permeate flux and assess the technical and economic feasibility. The optimum operating condition was to use 5 mg/L cationic polyacrylamide with 25 µm pore size and 0.1 m/s cross-flow velocity, under which a high harvesting efficiency of 95.2 %, a high average permeate flux of 55.5 m3/(m2 h) and a volumetric reduction factor of 118.9 were achieved. Algal floc analysis revealed that flocs formed by ferric chloride and polyaluminium sulfate tended to partially deconstruct into smaller pieces during the filtration process. In contrast, flocs formed by cationic polyacrylamide tended to aggregate into bigger flocs, which, when paired with the effect of flocculant dosage and membrane pore size, could explain the difference in filtration performance and membrane permeance. No negative effect on downstream technology was observed for the combined process. A significantly lowered estimated total cost of 0.139 $/kg under optimum operating condition was obtained compared to filtration without flocculation assisted (0.206 $/kg).


Assuntos
Microalgas , Floculação , Filtração , Cátions
6.
Ultrasound Med Biol ; 49(1): 375-379, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36283939

RESUMO

The aim of this study was to ascertain the safety of high-intensity focused ultrasound (HIFU) for high-grade cervical intraepithelial neoplasia grade 2/3 (CIN 2/3) in patients with fertility requirements. This was a prospective one-arm study. Consecutive CIN 2/3 patients diagnosed with histopathology were screened, enrolled and treated from September 2019 to September 2020 in the Affiliated Hospital of North Sichuan Medical College. All patients were treated with a combination of HIFU and antiviral treatment with REBACIN. The scheduled follow-up visits were 1 week, 1 mo, 3 mo, 6 mo and 12 mo after surgery. The primary outcomes included cure and human papillomavirus clearance rates. We screened 287 consecutive CIN 2/3 patients in our hospital, 29 of whom were enrolled and treated in this study. The cure rate reached 82.8% at 7 mo after treatment and 96.6% within 1 y. The HPV-negative rate reached 72.4% (21/29) around 6 mo after treatment, with mild side effects during and after the procedure. Our study suggests that in CIN 2/3 study participants with fertility requirements, HIFU + REBACIN therapy is a safe and effective therapeutic option with a high cure rate, HPV clearance and few side effects.


Assuntos
Neoplasia Intraepitelial Cervical , Infecções por Papillomavirus , Neoplasias do Colo do Útero , Feminino , Humanos , Neoplasia Intraepitelial Cervical/cirurgia , Neoplasia Intraepitelial Cervical/diagnóstico , Infecções por Papillomavirus/terapia , Estudos Prospectivos , Neoplasias do Colo do Útero/diagnóstico por imagem , Neoplasias do Colo do Útero/cirurgia , Papillomaviridae
7.
J Colloid Interface Sci ; 630(Pt A): 535-543, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36270174

RESUMO

Lithium-sulfur battery is one of the most promising candidates for next-generation energy storage systems, but the serious shuttle effect and sluggish reaction kinetics of polysulfides impair its practical applications. Herein, sulfur-deficient MoS2/carbon hollow nanospheres (MoS2-CHNs) are firstly synthesized by a NaCl-template pyrolysis and employed as sulfur host for lithium-sulfur batteries. TEM analysis reveals that carbon hollow nanospheres existing in the composite are the backbones that help to immobilize the ultrathin MoS2 nanosheets and ensure their large specific surface area. The MoS2 nanosheets consist of 5-10 layers of MoS2 with rich sulfur vacancies. The first principle calculation demonstrates that sulfur vacancy led to an intensively enhanced binding energy (-4.70 eV) towards Li2S6 compared to the pristine MoS2 (-1.57 eV). It suppressed the shuttle effect efficiently. The catalytic experiments reveal that MoS2-CHNs have a superior ability for the nucleation of Li2S and bidirectional electrocatalytic capability for the conversion of polysulfide. The large storage space inside MoS2-CHNs can work as a reservoir for intermediate polysulfides to substantially reduce the concentration overpotential. Due to this advantageous structural design of MoS2-CHNs electrode, its reversible capacity remains 1139 mAh g-1 after 100 cycles at 0.2C, and 600 mAh g-1 after 500 cycles at 5C with a sulfur loading of 5 mg cm-2. Even though the sulfur loading increases to 10 mg cm-2, the Li-S battery delivers a stable capacity of 978 mAh g-1 after 50 cycles at 0.2C. So the MoS2-CHNs demonstrate a promising application for high-energy Li-S batteries.


Assuntos
Carbono , Nanosferas , Molibdênio , Lítio , Enxofre
8.
Artigo em Inglês | MEDLINE | ID: mdl-36464270

RESUMO

This work aim to investigate the effects and mechanism of emodin in treating diabetic gastroenteropathy and colonic dysmotility in STZ+HS/HF diet induced diabetic gastroenteropathy rats. Diabetic colonic dysmotility model was established by high-fat /high-glucose (HS/HF) feeding combined with streptozotocin (STZ). Emodin was divided into high, medium and low dose groups. After eight weeks of intervention, fasting blood glucose (FBG) and body weight were measured. Gastrointestinal transmission time was evaluated. Serum vasoactive intestinal peptide (VIP) and substance P (SP) were detected. Colonic protein expression of selective autophagy adaptor proteins p62 and beclin1 were detected by immunohistochemistry. Colonic protein expression of beclin1, autophagy related gene 5 (Atg5), C-kit and p62 were detected by Western Blot. After treating with emodin, gastrointestinal transmission rate was improved. The expression of serum SP was increased and serum VIP was decreased. Colonic c-kit and p62 were up-regulated. The expressions of beclin1 and Atg5 were down-regulated. Emodin can improve colonic dysmotility and promote the recovery of colonic motility and intestinal defecation in diabetic rats. It's mechanism may involved with up-regulating the expression of C-kit and P62, down-regulating the expression of Beclin1 and Atg5 in colon, which are associated with colon over-autophagy of ICC.

9.
Front Endocrinol (Lausanne) ; 13: 914872, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36465647

RESUMO

Purpose: Exercise therapy and key regulators of bone quality exert anti-hyperglycemic effects on type 2 diabetes mellitus (T2DM) mice. A number of programs have been reported to have an effect on bone disease in T2DM. Major unanswered questions concern the potential correlation of exercise with the improvement of bone quality in T2DM mice and how the nonlinear optical properties of bone are correlated with changes to its crystal structure. Methods: Subjects were randomly divided into six groups: 1) control (C) group, which was fed a normal diet (n = 8); 2) T2DM quiet group, which was given a high-fat diet and quiet (n = 8); 3) T2DM plus swimming (T2DM+S) group, which received T2DM and swim training (n = 8); 4) T2DM plus resistance exercise (T2DM+RE) group, which was given T2DM and resistance exercise (n = 8); 5) T2DM plus aerobic exercise (T2DM+AE) group, with T2DM and medium-intensity treadmill exercise (n = 8); and 6) T2DM plus high-intensity interval training (T2DM+HIIT), with T2DM and high-intensity variable-speed intervention (n = 8). The levels of runt-related transcription factor 2 (RUNX2), osterix (OSX), and alkaline phosphatase (ALP), as well as the bone microstructure and morphometry, were measured at the end of the 8-week exercise intervention. Results: Compared with the C group, the bone microstructure indexes [bone mineral density (BMD), bone volume/tissue volume (BV/TV), cortical thickness (Ct.Th), and connectivity density (Conn.D)], the bone biomechanical properties (maximum load, fracture load, yield stress, and elastic modulus), and the osteogenic differentiation factors (RUNX2, OSX, and BMP2) of the T2DM group were significantly decreased (all p < 0.05). Compared with the T2DM group, there were obvious improvements in the osteogenic differentiation factor (OSX) and Th.N, while the separation of trabecular bone (Tb.Sp) decreased in the T2DM+AE and T2DM+HIIT groups (all p < 0.05). In addition, the bone microstructure indicators BV/TV, tissue mineral density (TMD), Conn.D, and degree of anisotropy (DA) also increased in the T2DM+HIIT group, but the yield stress and Ct.Th deteriorated compared with the T2DM group (all p < 0.05). Compared with the T2DM+S and T2DM+RE groups, the BV/TV, trabecular number (Tb.N), Tb.Sp, and Conn.D in the T2DM+AE and T2DM+HIIT groups were significantly improved, but no significant changes in the above indicators were found between the T2DM+S and T2DM+RE groups (all p < 0.05). In addition, the BMD and the expression of ALP in the T2DM+AE group were significantly higher than those in the T2DM+HIIT group (all p < 0.05). Conclusion: There was a significant deterioration in femur bone mass, trabecular bone microarchitecture, cortical bone geometry, and bone mechanical strength in diabetic mice. However, such deterioration was obviously attenuated in diabetic mice given aerobic and high-intensity interval training, which would be induced mainly by suppressing the development of T2DM. Regular physical exercise may be an effective strategy for the prevention of not only the development of diabetes but also the deterioration of bone properties in patients with chronic T2DM.


Assuntos
Diabetes Mellitus Experimental , Diabetes Mellitus Tipo 2 , Osteoporose , Camundongos , Animais , Humanos , Subunidade alfa 1 de Fator de Ligação ao Core , Diabetes Mellitus Tipo 2/terapia , Osteogênese , Fêmur , Osteoporose/etiologia , Osteoporose/terapia , Terapia por Exercício , Fosfatase Alcalina
10.
Artigo em Inglês | MEDLINE | ID: mdl-36472735

RESUMO

Although the partial nitrification-anammox process (PN-A) has achieved great success in nitrogen removal for the high ammonium concentration wastewater, its application is still limited in low ammonium concentration wastewater treatment due to its instability and low nitrogen removal efficiency. In this study, a sequencing batch reactor (SBR) with continuous aeration was employed to enrich ammonia oxidation bacteria (AOB) and suppress nitrite oxidation bacteria (NOB) first; then, the SBR was operated intermittently aerated SBR (IASBR), to which the anammox granular sludge (AMX) was added to achieve complete autotrophic nitrogen removal under low influent ammonium concentration of 100 mg/L. A mathematical model was used to optimize the IASBR aeration strategy to achieve sub-optimal nitrogen removal. The experimental results showed that high nitrite accumulation efficiency (above 80%) in the SBR and a fast start-up within 100 days and a stable TN (total nitrogen) removal efficiency of 70% were achieved in the IASBR. Meanwhile, the simulation results indicated that keeping aeration duration at 4 h, kLa (oxygen transfer coefficient) at 50 day-1, or aeration duration at 2.5 h, kLa at 80 day-1 could obtain a higher total nitrogen removal efficiency (TNR) (TNR > 80%), and the TN removal could also be improved by increasing hydraulic retention time (HRT) under the optimal oxygen supply rate.

11.
Poult Sci ; 102(1): 102240, 2022 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-36334472

RESUMO

In recent years, the utilization of antibiotics in animal feed has been restricted, probiotics have been increasingly used to replace antibiotics in maintaining animal health. The aim of this study was to screen and evaluate probiotics with excellent probiotic potential from the gut of healthy goslings for clinical application. Thirteen strains of Bacillus (named AH-G201 to AH-G2013), including 2 strains of Bacillus subtilis (B. subtilis), 6 strains of Bacillus licheniformis (B. licheniformis) and 5 strains of Bacillus amyloliquefaciens (B. amyloliquefaciens), were isolated and identified. Then, acid and bile salts tolerance tests were performed to screen probiotics strains that could survive under different environments. The effects of screened probiotics on the growth of pathogenic Escherichia coli (E. coli) and Salmonella were assessed. Furthermore, we performed the drug resistance tests and safety tests in animals. The results showed that B. Subtilis AH-G201, B. licheniformis AH-G202 and AH-G204 exhibited higher gastrointestinal resistance under in vitro conditions, and showed a moderate level of resistance to the tested antibiotics. Importantly, AH-G201 and AH-G202 showed 24 to 60% inhibition rate against pathogenic E. coli and Salmonella. Moreover, the safety analysis of AH-G201 and AH-G202 suggested that the 2 probiotics strains have no adverse effects on body weight gain and feed intake in the broilers, and in addition, they have significantly improved growth performance. Finally, we analyzed effects of B. Subtilis AH-G201and B. licheniformis AH-G202 on growth performance, immune organ index and the feces microbes of broilers. The results showed that broilers fed with high doses (5 × 109 CFU/mL, for single strain) of a mixture of AH-G201 and AH-G202 exhibited good growth performance, and exhibited the greatest gain in spleen weight and the highest lactic acid bacteria counts. These findings indicate that the combined addition of B. Subtilis AH-G201 and B. licheniformis AH-G202 has the potential to replace antibiotics and to improve the growth performance of broilers.

12.
Crit Rev Food Sci Nutr ; : 1-22, 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36330804

RESUMO

As one of the major short-chain fatty acids produced via microbial fermentation, butyrate serves as not only a preferred energy substrate but also an important signaling molecule. Butyrate concentrations in circulation, tissues, and gut luminal contents have important pathophysiological implications. The genetic capacity of butyrate biosynthesis by the gut microbiota is frequently compromised during aging and various disorders, such as inflammatory bowel disease, metabolic disorders and colorectal cancer. Substantial efforts have been made to identify potent butyrogenic substrates and butyrate-hyperproducing bacteria to compensate for butyrate deficiency. Interindividual butyrogenic responses exist, which are more strongly predicted by heterogeneity in the gut microbiota composition than by ingested prebiotic substrates. In this review, we catalog major food types rich in butyrogenic substrates. We also discuss the potential of butyrogenic foods with proven properties for promoting gut health and disease management using findings from clinical trials. Potential limitations and constraints in the current research are highlighted. We advocate a precise nutrition approach in designing future clinical trials by prescreening individuals for key gut microbial signatures when recruiting study volunteers. The information provided in this review will be conducive to the development of microbiota engineering approaches for enhancing the sustained production of butyrate.

13.
Photodiagnosis Photodyn Ther ; : 103200, 2022 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-36384211

RESUMO

BACKGROUND: Moderate-to-severe acne affects people's health and quality of life. As first-line therapeutic medications, isotretinoin and antibiotics was used to treat moderate-to-severe acne.However,some patient does not tolerate pills. Thus, It is important to improve therapeutic tools for those people. Now, there are more and more clinical studies on the treatment of moderate-to-severe acne treated by the combination of fire needle and ALA-PDT, which provides us with a new idea for the treatment of moderate-to-severe acne. It is necessary to assess the clinical evidence supporting the use of fire needle combined with ALA-PDT in the treatment of moderate to severe acne in order to offer a foundation for clinical practice.This study evaluated the safety and effectiveness in the treatment of moderate-to-severe acne by the combination with fire needle and ALA-PDT. METHODS: By July 2022, search PubMed, the Chinese Biomedical Literature Databas, the Cochrane Library, the Chinese Scientific Journal Database, the China National Knowledge Infrastructure, the Web of Science Datebase, Embase Datebase,VIP Database and WanFang Database. To gather RCTs of fire needle combination with ALA-PDT for the treatment of moderate-to-severe acne. A meta-analysis was performed according to the Handbook guidelines of Cochrane. Study selection, data extraction, and risk of bias evaluation were all governed by two reviewers, with the help of a third reviewer if needed. The meta-analysis was carried out with Review Manager Software 5.4. RESULTS: There were a total of 9 RCTs with 862 participants. Clinical efficacy was recorded in nine trials, GAGS score was published in three studies, adverse events were documented in five studies, and recurrence rate was reported in two studies. Treatment lasted between four and twelve weeks. Combination therapy outperformed monotherapy in terms of clinical efficacy (OR:3.73; 95% CI:2.51, 5.53; p<0.00001). Additional subgroup analysis revealed that the combination therapy outperformed ALA-PDT alone in terms of clinical effect (OR: 3.20; 95% CI: 2.05, 4.99; p<0.00001). Additionally, combination therapy outperformed fire needle alone in terms of clinical efficacy (OR:3.74; 95% CI: 2.55, 5.48; p<0.00001). Studies have also indicated that combination therapy has a stronger benefit in lowering the GAGS score (MD:-3.35; 95% CI:-4.62, -2.09; p<0.00001). Additionally, there was no discernible difference in the occurrence of adverse events between the combined treatments and monotherapy (OR:1.43; 95% CI: 0.76, 2.69; p=0.26), and the combined treatment was able to control the recurrence rate (OR:0.18; 95% CI: 0.07, 0.45; P=0.0002). CONCLUSIONS: The efficacy of fire needle combined with ALA-PDT in the treatment of moderate-to-severe acne is superior to that of ALA-PDT or fire needle alone. However, the conclusions of this study must be interpreted carefully due to the high risk and ambiguity of bias of the included trials.

14.
Artigo em Inglês | MEDLINE | ID: mdl-36433823

RESUMO

BACKGROUND: Pheochromocytomas and paragangliomas (PPGL) are rare neuroendocrine tumors. Metastases develop in 15-20%. The American Joint Committee on Cancer (AJCC) established inaugural guidelines for PPGL tumor-node-metastasis (TNM) staging. The objective of this analysis is to investigate the associations between TNM staging and overall survival (OS). METHODS: We retrospectively applied the TNM staging at the time of diagnosis of the primary tumor. The primary outcome was OS. Unadjusted survival rates were estimated by the Kaplan-Meier method. Cox proportional hazards regression models were used to evaluate the associations between OS and covariates of interest. RESULTS: The study included 458 patients. Median OS was 18.0 (95% CI: 15.6-not reached) years. At diagnosis, 126 (27.5%) tumors were stage I, 213 (46.5%) were stage II, 47 (10.3%) were stage III, and 72 (15.7%) were stage IV. The 10-year OS probabilities were 0.844 (95% CI: 0.768-0.928) for patients with stage I tumors, 0.792 (95% CI: 0.726-0.865) for stage II, 0.595 (95% CI: 0.435-0.813) for stage III, and 0.221 (95% CI: 0.127-0.384) for stage IV. Compared with stage I, the hazard ratios (HR) for death were 1.50 (0.87-2.57) for stage II, 2.85 (1.45-5.63) for stage III, and 8.88 (5.16-15.29) for stage IV (p < 0.001). Compared with patients with no germline mutations, those with RET 634/918 had better OS (HR: 0.28, 95% CI: 0.12-0.69). Other germline mutations, including SDHB, did not exhibit worse OS than patients with metastasis and sporadic disease. CONCLUSIONS: OS rates correlated with the recently developed AJCC TNM staging and was not worse in hereditary disease. Stage IV disease exhibited a significantly shorter OS compared with stages I-III. Future staging systems could be adjusted to better separate stages I and II.

15.
Ying Yong Sheng Tai Xue Bao ; 33(11): 3116-3126, 2022 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-36384846

RESUMO

Antibiotic resistance genes (ARGs) in soil pose a major challenge to global environment and health. The development of effective technologies to reduce their negative effects has implications for maintaining soil health and human health. Biochar would be a suitable control material due to its characteristics of high carbon content, large surface area, excellent adsorption capacity, and economic advantages. There are three mechanisms underlying its negative effects on the abundance of ARGs: 1) adsorption of certain pollutants (e.g., antibiotics and heavy metals) to reduce the co-selective pressure of ARGs; 2) alteration of microbial composition through altering soil physico-chemical properties, and thereby limiting the ability of bacteria to undergo horizontal transfer of ARGs; 3) direct impairment of horizontal gene transfer by the adsorption of horizontal transfer vectors such as plasmids, transposons, and integrons. However, the negative effect of biochar depends on the source of material, pyrolysis process, and its amount added. Furthermore, field aging of biochar may reduce its ability to block ARGs. Endogenous contaminants of biochar, such as polycyclic aromatic hydrocarbons and heavy metals, may cause the enrichment of specific antibiotic-resistant bacteria in the environment or induce horizontal gene transfer. In further studies, suitable biochar should be selected according to soil environments, and biochar aging control measures should be taken to improve its retarding effect on ARGs.


Assuntos
Metais Pesados , Solo , Humanos , Solo/química , Antibacterianos/farmacologia , Microbiologia do Solo , Resistência Microbiana a Medicamentos/genética , Metais Pesados/análise , Bactérias/genética
16.
Front Immunol ; 13: 978909, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36341328

RESUMO

Background: Cuproptosis is a newly discovered programmed cell death dependent on overload copper-induced mitochondrial respiration dysregulation. The positive response to immunotherapy, one of the most important treatments for invasive breast cancer, depends on the dynamic balance between tumor cells and infiltrating lymphocytes in the tumor microenvironment (TME). However, cuproptosis-related genes (CRGs) in clinical prognosis, immune cell infiltration, and immunotherapy response remain unclear in breast cancer progression. Methods: The expression and mutation patterns of 12 cuproptosis-related genes were systematically evaluated in the BRCA training group. Through unsupervised clustering analysis and developing a cuproptosis-related scoring system, we further explored the relationship between cuproptosis and breast cancer progression, prognosis, immune cell infiltration, and immunotherapy. Results: We identified two distinct CuproptosisClusters, which were correlated with the different patterns between clinicopathological features, prognosis, and immune cell infiltration. Moreover, the differences of the three cuproptosis-related gene subtypes were evaluated based on the CuproptosisCluster-related DEGs. Then, a cuproptosis-related gene signature (PGK1, SLC52A2, SEC14L2, RAD23B, SLC16A6, CCL5, and MAL2) and the scoring system were constructed to quantify the cuproptosis pattern of BRCA patients in the training cohort, and the testing cohorts validated them. Specifically, patients from the low-CRG_score group were characterized by higher immune cell infiltration, immune checkpoint expression, immune checkpoint inhibitor (ICI) scores, and greater sensitivity to immunotherapy. Finally, we screened out RAD23B as a favorable target and indicated its expression was associated with breast cancer progression, drug resistance, and poor prognosis in BRCA patients by performing real-time RT-PCR, cell viability, and IC50 assay. Conclusions: Our results confirmed the essential function of cuproptosis in regulating the progression, prognosis, immune cell infiltration, and response to breast cancer immunotherapy. Quantifying cuproptosis patterns and constructing a CRG_score could help explore the potential molecular mechanisms of cuproptosis regulating BRCA advancement and provide more effective immunotherapy and chemotherapy targets.


Assuntos
Neoplasias da Mama , Microambiente Tumoral , Humanos , Feminino , Microambiente Tumoral/genética , Prognóstico , Imunoterapia , Inibidores de Checkpoint Imunológico , Proteínas Proteolipídicas Associadas a Linfócitos e Mielina
17.
Medicine (Baltimore) ; 101(46): e31822, 2022 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-36401478

RESUMO

BACKGROUND: In recent years, with the increase of patients with coronary heart disease, the number of patients with heart failure (HF) has also gradually increased. Coronary heart disease is one of the most common causes of HF. Anxiety and depression are frequent psychological disorders in patients with HF. Studies have shown that anxiety and depression can affect the quality of life of patients with HF, and can increase hospitalization and mortality. Conventional pharmacotherapy and psychotherapy have certain limitations. Acupuncture has therapeutic effects on heart disease, anxiety and depression, and has been widely used to relieve symptoms in patients with HF. This protocol aims to evaluate the safety and efficacy of acupuncture for anxiety and depression in patients with HF. METHODS: We will search the following databases: PubMed, Web of Science, Springer Cochrane Library, EMBASE, MEDLINE, WHO international clinical trials registry platform, China National Knowledge Infrastructure database, Wan Fang database, Chinese scientific journal database and Chinese Biomedical Literature Database. The databases will be searched from initiate to October 1, 2022. Two reviewers will screen and document eligible studies based on inclusion and exclusion criteria. Two reviewers will independently perform data analysis and bias risk assessment. Review Manager version 5.4 software will be used for meta-analysis. RESULTS: This study will explore the efficacy and safety of acupuncture for anxiety and depression in patients with HF. CONCLUSION: The results of this study will provide high-quality evidence for evaluating the safety and efficacy of acupuncture for anxiety and depression in patients with HF.


Assuntos
Terapia por Acupuntura , Ansiedade , Depressão , Insuficiência Cardíaca , Humanos , Terapia por Acupuntura/efeitos adversos , Ansiedade/etiologia , Ansiedade/terapia , Depressão/etiologia , Depressão/terapia , Insuficiência Cardíaca/complicações , Insuficiência Cardíaca/terapia , Metanálise como Assunto , Qualidade de Vida , Revisões Sistemáticas como Assunto
18.
Microb Ecol ; 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36326874

RESUMO

The emergence of the plasmid-mediated tigecycline resistance gene tetX family in pig farms has attracted worldwide attention. The use of tetracycline antibiotics in pig farms has a facilitating effect on the prevalence of the tetX family, but the relationship among its presence, expression, and resistance phenotype in resistant bacteria is unknown. In this study, the presence and expression characteristics of tetracycline resistance genes (TRGs) in 89 strains of doxycycline-resistant E. coli (DRE) isolated from pig manure samples from 20 pig farms under low concentrations of doxycycline stress (2 µg/mL) were analyzed. The detection rate of tetO was 96.63%, which is higher than those of other TRGs, such as tetA (94.38%), tetX (76.40%), tetB (73.03%), and tet(X4) (69.66%). At least three TRG types were present in DRE strains, which thus showed extensive resistance to tetracycline antibiotics, and 37% of these strains were resistant to tigecycline. In the presence of a low concentration of doxycycline, tetA played an important role, and the expression and existence ratio of TRGs indicated low expression of TRGs. Furthermore, the doxycycline resistance of DRE was jointly determined by the total absolute abundance of TRGs, and the absolute abundance of tetX and tet(X4) was significantly positively associated with tigecycline resistance in DRE (P < 0.05). Overall, DRE isolated from swine manure is an important reservoir of the tetX family, which suggests that DRE in swine manure has a high risk of tigecycline resistance, poses a potential threat to human health, and should be of public concern.

19.
Artigo em Inglês | MEDLINE | ID: mdl-36327011

RESUMO

PURPOSE: This study aimed to determine if layer-specific strain (LSS) can be used to evaluate and predict left ventricular (LV) recovery in patients with multi-vessel coronary artery disease (CAD) undergoing hybrid coronary revascularization (HCR) using speckle tracking echocardiography (STE). METHODS: A total of 187 consecutive CAD patients who received HCR in our hospital were prospectively enrolled. 30 healthy individuals with matched age and gender were enrolled as a control group. Echocardiography was performed for CAD patients before and 1, 2, and 6 months after HCR. Comprehensive conventional and LSS echocardiography parameters were collected. LV recovery was defined as improvement in LV ejection fraction (LVEF) > 5% at 6-months follow-up compared with baseline. Logistic regression analysis was used to test the correlates of LV recovery. Receiver operating characteristic curve analysis was used to determine the optimal cutoff value of correlates for predicting LV recovery. RESULTS: LVEF and LV strain in CAD patients were significantly decreased compared with control subjects. Endocardial global longitudinal strain (Endo-GLS) improved significantly at 1-month follow-up (14.2 ± 1.6% vs. 13.8 ± 1.5%, P < 0.05), and LVGLS and global circumferential strain (GCS) improved significantly at 2-months follow-up. Multivariate regression revealed that Endo-GLS, GLS, and SYNTAX score before HCR were independently correlated to LV recovery. Endo-GLS had an optimal cutoff value of 13.2% for predicting LV recovery with sensitivity of 91% and specificity of 78%. CONCLUSION: LV myocardial systolic function in CAD patients was impaired before HCR and significantly improved after HCR. Endo-GLS was independently correlated to and has optimal predictive value for LV recovery.

20.
Front Aging Neurosci ; 14: 981451, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36389060

RESUMO

Background: Alzheimer's disease (AD) and frontotemporal lobar degeneration (FTLD) are the two most common forms of neurodegenerative dementia. Although both of them have well-established diagnostic criteria, achieving early diagnosis remains challenging. Here, we aimed to make the differential diagnosis of AD and FTLD from clinical, neuropsychological, and neuroimaging features. Materials and methods: In this retrospective study, we selected 95 patients with PET-CT defined AD and 106 patients with PET-CT/biomarker-defined FTLD. We performed structured chart examination to collect clinical data and ascertain clinical features. A series of neuropsychological scales were used to assess the neuropsychological characteristics of patients. Automatic tissue segmentation of brain by Dr. Brain tool was used to collect multi-parameter volumetric measurements from different brain areas. All patients' structural neuroimage data were analyzed to obtain brain structure and white matter hyperintensities (WMH) quantitative data. Results: The prevalence of vascular disease associated factors was higher in AD patients than that in FTLD group. 56.84% of patients with AD carried at least one APOE ε4 allele, which is much high than that in FTLD patients. The first symptoms of AD patients were mostly cognitive impairment rather than behavioral abnormalities. In contrast, behavioral abnormalities were the prominent early manifestations of FTLD, and few patients may be accompanied by memory impairment and motor symptoms. In direct comparison, patients with AD had slightly more posterior lesions and less frontal atrophy, whereas patients with FTLD had more frontotemporal atrophy and less posterior lesions. The WMH burden of AD was significantly higher, especially in cortical areas, while the WMH burden of FTLD was higher in periventricular areas. Conclusion: These results indicate that dynamic evaluation of cognitive function, behavioral and psychological symptoms, and multimodal neuroimaging are helpful for the early diagnosis and differentiation between AD and FTLD.

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