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1.
J Colloid Interface Sci ; 560: 284-292, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31670101

RESUMO

In this work, the chitosan wrapped multiwalled carbon nanotubes (MWCNTs-CS) composited material was prepared by surface deposition and crosslinking method. This mild process can maintain the unique properties of the original carbon nanotubes intact. The morphological character of MWCNTs-CS was examined via Fourier transform infrared spectroscopy and field emission scanning electron microscopy. MWCNTs-CS was used as sensing film to fabricate quartz crystal microbalance (QCM) humidity sensors. The optimized sensor possesses high response sensitivity (46.7 Hz/% RH), negligible humidity hysteresis (around 1.1% RH), quick response and recovery time (75 s/34 s), and remarkable reversibility, repeatability, long-term stability and selectivity. Langmuir adsorption isotherm model was used to study the adsorption process of water molecules on MWCNTs-CS film, and the Gibbs free adsorption energy was calculated as -21.85 kJ/mol. By combining the good mechanic properties of MWCNTs and the high hydrophilia of chitosan, the MWCNTs-CS composites are promising for humidity sensing application.

2.
Curr HIV Res ; 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31654514

RESUMO

BACKGROUND: Antiretroviral therapy (ART) is associated with lipid abnormalities that contribute to increasing risk of cardiovascular (CV) events among patients with human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS). Although disorders of lipid metabolism associated with ART have been described before in developed countries, data on lipid profile disorders associated with ART use in China are limited. This study aimed to examine the changes in lipid profile among patients with HIV/AIDS who initiated lopinavir/ritonavir LPV/r or efavirenz (EFV)-based antiretroviral treatment regimens, which continue to be widely used China and other developing countries. METHODS: This is a retrospective, matched case control study of HIV-positive patients initiating either LPV/r or EFV regimens at the Beijing You'an Hospital, Capital Medical University between July 2012 and January 2017. Generalized estimating equations were used to compare the differences in total cholesterol [TC], triglycerides [TG], low-density lipoprotein-cholesterol [LDL-C], and high-density lipoprotein-cholesterol [HDL-C] at baseline and up to 24-months after ART initiation between the two treatment arms. RESULTS: Baseline characteristics, including age, sex, CD4 cell count, viral load, and serum lipids, which were comparable between the two groups. The LPV/r-based regimen group had increased TC, TG, HDL-C, and LDL-C after 24-months of treatment. In the EFV-regimen group, TC, HDL-C, and LDL-C were increased compared to baseline, while the TC/HDL-C ratio decreased, and TG did not change significantly. After 24-months of treatment, the percentage of patients with dyslipidemia in the LPV/r group was much higher than in the EFV group (84.0% vs. 52.6%, P<0.001), and 17(10%) patients on LPV/r-based regimens had severe dyslipidemia. Patients on LPV/r-based regimens were at increased odds of hypercholesterolemia (odds ratio [OR]=1.709, P=0.038), hypertriglyceridemia (OR=4.315, P<0.001), and high TC/HDL-C ratio (OR=1.951, P=0.003). However, no significant difference was found in HDL-C (OR=1.246, P=0.186) or LDL-C (OR=1.253, P=0.410) between the treatment groups. CONCLUSIONS: Both LPV/r or EFV treatment regimens impacted patients' lipid profile. Compared to EFV-based regimens, patients on LPV/r-based regimens had increased odds of dyslipidemia, such as hypercholesterolemia, hypertriglyceridemia, or high TC/HDL-C ratio; however, there was no obvious effect on LDL-C, which is more relevant to the development of cardiovascular disease.

3.
Nat Commun ; 10(1): 4574, 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31594929

RESUMO

Several recent studies have shown the presence of genes for the key enzyme associated with archaeal methane/alkane metabolism, methyl-coenzyme M reductase (Mcr), in metagenome-assembled genomes (MAGs) divergent to existing archaeal lineages. Here, we study the mcr-containing archaeal MAGs from several hot springs, which reveal further expansion in the diversity of archaeal organisms performing methane/alkane metabolism. Significantly, an MAG basal to organisms from the phylum Thaumarchaeota that contains mcr genes, but not those for ammonia oxidation or aerobic metabolism, is identified. Together, our phylogenetic analyses and ancestral state reconstructions suggest a mostly vertical evolution of mcrABG genes among methanogens and methanotrophs, along with frequent horizontal gene transfer of mcr genes between alkanotrophs. Analysis of all mcr-containing archaeal MAGs/genomes suggests a hydrothermal origin for these microorganisms based on optimal growth temperature predictions. These results also suggest methane/alkane oxidation or methanogenesis at high temperature likely existed in a common archaeal ancestor.

4.
Aging (Albany NY) ; 112019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31649207

RESUMO

The therapeutic strategies for advanced gastric cancer (GC) remain unsatisfying and limited. Therefore, it is still imperative to fully elucidate the mechanisms underlying GC aggressive progression. The prognostic value and biological functions of low density lipoprotein receptor class A domain containing protein 2 (LDLRAD2) in GC have never been studied yet. We found that LDLRAD2 expression was significantly upregulated in GC and closely correlated with poor prognosis in GC patients. Functionally, LDLRAD2 promoted epithelial-mesenchymal transition, migration and invasion, and metastasis of GC cells. Mechanistically, LDLRAD2 interacted with and inhibited Axin1 from binding to cytoplasmic ß-catenin, which facilitated the nuclear translocation of ß-catenin, thereby activating Wnt/ß-catenin pathway. Inhibition of ß-catenin activity markedly abolished LDLRAD2-induced migration, invasion and metastasis. Together, these results suggested that LDLRAD2 contributed to invasion and metastasis of GC through activating Wnt/ß-catenin pathway. LDLRAD2/ Wnt/ß-catenin axis may be a potential therapeutic target for GC treatment.

5.
Nanoscale ; 11(40): 18758-18768, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31591618

RESUMO

The search for suitable anodes with good performance is a key challenge for rechargeable Li- and Na-ion batteries (LIBs and NIBs). In this work, we adopt first-principles calculations and ab initio molecular dynamics simulations to investigate the ion transport mechanism and potential of C2N stoichiometric nitrogenated holey graphite (C2N-NHG) as a promising anode material for LIBs and NIBs. Although huge in-plane diffusion barriers for both Li and Na ions restrict the application of the C2N-NHG monolayer as an effective anode, Li and Na ions are found to exhibit facile out-of-plane ion transport in the most stable layered AD stacking C2N-NHG. The fully lithiated and sodiated cases of LiC2N and Na0.67C2N show reversible specific capacities up to 587 mA h g-1 and 353 mA h g-1, low chemical potentials of 0.12 V and 0.25 V, and small volume expansions of 7.16% and 13.54%, respectively. Meanwhile, the out-of-plane collective diffusion reduces Li/Na collective migration barriers to 0.23 eV and 0.18 eV. These findings suggest that AD stacking C2N-NHG, with metallic properties after lithiation and sodiation processes, high specific capacity, low open circuit voltage, small volume expansion, and low collective migration barriers, has the potential to serve as a promising high-rate anode material for LIBs and NIBs with large energy density and power density. The calculations reveal that the novel out-of-plane diffusion behaviour plays a crucial role in Li/Na ion transport in holey layered materials.

6.
ACS Appl Mater Interfaces ; 11(43): 39841-39847, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31596067

RESUMO

Developing hollow self-supported nanotube arrays with hierarchical microporous and abundant multiactive sites shows great promise for oxygen evolution reaction (OER) electrocatalysis. Herein, a facile and low-cost strategy of NiCo2S4 hollow nanotubes arrays decorated with CeOx nanoparticles (NPs) assembled on a flexible support carbon cloth (CC) for enhanced OER performance is reported. The obtained hierarchical nanoarrays CeOx/NiCo2S4/CC exhibited excellent activity toward OER with an overpotential (270 mV) at 10 mA cm-2, relatively weak Tafel slope, and distinguished durability. CeOx/NiCo2S4/CC nanoarrays not only provide fast electronic transmission and well-defined connection to the substrate but also defective sites and electron transfer by the introduction of CeOx NPs. This new strategy was offered to construct low-cost and effectively hierarchical structural electrocatalysts containing rare-earth species.

7.
Brain Res ; : 146518, 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31647899

RESUMO

Chloroquine, a prototype anti-malaria drug, has been reported to possess anti-inflammatory effects. Moreover, chloroquine pretreatment could improve DNA damage repair. It is therefore reasonable to hypothesize that chloroquine pretreatment could attenuate ischemia/reperfusion injury in the brain. Considering the fact that chloroquine could also improve glucose metabolism, we speculated that the potential effects of chloroquine on ischemia/reperfusion injury might be particularly pronounced in diabetic mice. In this study, chloroquine pretreatment protected neurons from Oxygen Glucose Deprivation (OGD) induced cytotoxicity and apoptosis. In vivo, Ob/ob mice and wildtype (WT) mice were pretreated with chloroquine for 3 weeks. Then, ischemic stroke was induced by 60 min Middle Cerebral Artery Occlusion (MCAO). We found that chloroquine pretreatment normalized blood glucose in diabetic ob/ob mice, and reduced cerebral damage after ischemic stroke especially for diabetic mice. In addition, chloroquine pretreatment reduced High-mobility group box 1 (HMGB1) content in the cerebrospinal fluid (CSF) and serum and lowered myeloperoxidase (MPO) activity and inflammatory cytokines gene expression both in the ob/ob diabetic mice and WT mice. Moreover, harmful DNA damage-signaling responses, including PARP activation and p53 activation, were also attenuated by chloroquine pretreatment in these two kinds of mice. In conclusion, chloroquine pretreatment could reduce cerebral damage after ischemic stroke especially in diabetic mice through multiple mechanisms, which include reducing neural cell DNA injury, restoring euglycemia and anti-inflammatory effects. The findings may provide potential for the development of chloroquine in the prevention and treatment of stroke in diabetic high-risk patients.

8.
Bioinformatics ; 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31593245

RESUMO

SUMMARY: The biosynthetic ability of living organisms has important applications in producing bulk chemicals, biofuels and natural products. Based on the most comprehensive biosynthesis knowledgebase, a computational system, BCSExplorer, is proposed to discover the unexplored chemical space using nature's biosynthetic potential. BCSExplorer first integrates the most comprehensive biosynthetic reaction database with 280 000 biochemical reactions and 60 000 chemicals biosynthesized globally over the past 130 years. Second, in this study, a biosynthesis tree is computed for a starting chemical molecule based on a comprehensive biotransformation rule library covering almost all biosynthetic possibilities, in which redundant rules are removed using a new algorithm. Moreover, biosynthesis feasibility, drug-likeness and toxicity analysis of a new generation of compounds will be pursued in further studies to meet various needs. BCSExplorer represents a novel method to explore biosynthetically available chemical space. AVAILABILITY: BCSExplorer is available at: http://www.rxnfinder.org/bcsexplorer/. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

9.
Plant Biotechnol J ; 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31618513

RESUMO

Viral diseases severely affect crop yield and quality, thereby threatening global food security. Genetic improvement of plant virus resistance is essential for sustainable agriculture. In the last decades, several modern technologies were applied in plant antiviral engineering. Here we summarized breakthroughs of the two major antiviral strategies, RNA silencing and genome editing. RNA silencing strategy has been used in antiviral breeding for more than thirty years, and many crops engineered to stably express small RNAs targeting various viruses have been approved for commercial release. Genome editing technology has emerged in the past decade, especially CRISPR/Cas, which provides new methods for genetic improvement of plant virus resistance and accelerates resistance breeding. Finally, we discuss the potential of these technologies for breeding crops, and the challenges and solutions they may face in the future.

10.
Nat Methods ; 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31659327

RESUMO

Two-photon microscopy is a mainstay technique for imaging in scattering media and normally provides frame-acquisition rates of ~10-30 Hz. To track high-speed phenomena, we created a two-photon microscope with 400 illumination beams that collectively sample 95,000-211,000 µm2 areas at rates up to 1 kHz. Using this microscope, we visualized microcirculatory flow, fast venous constrictions and neuronal Ca2+ spiking with millisecond-scale timing resolution in the brains of awake mice.

11.
Water Res ; 168: 115160, 2019 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-31614233

RESUMO

Aquatic ecosystems have been increasingly threatened by anthropogenic activities, e.g., wastewater discharge and farm operation. Several methods are adopted to evaluate the effects of anthropogenic activities on biological risk in the environment, such as qPCR and amplicon next-generation sequencing. However, these methods fall short of providing genomic information of target species, which is vital for risk assessment from genomic aspect. Here, we developed a novel approach integrating metagenomic analysis and flow cytometry to identify and quantify potential pathogenic antibiotic resistant bacteria (PARB; carrying both antibiotic resistance genes (ARGs) and virulence factor genes (VFGs)) in the environment, which are of particular concern due to their infection ability and antibiotic resistance. Based on the abundance/density of PARB, we evaluated microbiological risk in a river impacted by both municipal drainage and agriculture runoff. We collected samples upstream (mountainous area) as the control. Results showed that 81.8% of dominant PARB (33) recovered using our approach were related to known pathogenic taxa. In addition, intragenomic ARGs-VFGs coexistence patterns in the dominant Pseudomonas genomes (20 out of 71 PARB) showed high similarity with the most closely related Pseudomonas genomes from the NCBI RefSeq database. These results reflect acceptable reliability of the approach for (potential) pathogen identification in environmental samples. According to the PARB density, microbiological risk in samples from the agricultural area was significantly higher than in samples from the urban area. We speculated that this was due to the higher antibiotic usage in agriculture as well as intragenomic ARGs-VFGs co-evolution under antibiotic selective pressure. This study provides an alternative approach for the identification and quantification of PARB in aquatic environments, which can be applied for microbiological risk assessment.

12.
BMC Immunol ; 20(1): 38, 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31651258

RESUMO

BACKGROUND: Helios has been reported to stabilize regulatory T (Treg) suppressive function. Programmed cell death protein 1 (PD-1) expression in three human monocyte subsets modulates immune responses. Recently, our team reported that three monocyte subsets are associated with T helper cell differentiation in HIV-1-infected patients. Until now, the effects of monocyte subsets and their PD-1 expression on Foxp3+Helios+ Treg cells have not been fully characterized, especially during acute HIV-1 infection. RESULTS: The frequency of Foxp3+Helios+CD45RA+ Treg cells is significantly higher in patients with acute HIV-1 infection than those of healthy controls and chronic HIV-1-infected patients undergoing combined antiretroviral therapy. The frequency of Foxp3+Helios+CD45RA+ Treg cells is inversely correlated with CD4 T-cell counts and the CD4/CD8 ratio in chronic HIV-1-infected patients. During acute HIV-1 infection, the frequency of Foxp3+Helios+CD45RA+ Treg cells is inversely correlated with the frequency of the intermediate CD14++CD16+ monocyte subset, but positively correlated with PD-1 expression in both intermediate CD14++CD16+ and non-classical CD14+CD16++ monocyte subsets. CONCLUSIONS: In this study, the perturbations of Foxp3+Helios+ Treg cells were characterized, and the association between monocyte subsets and their PD-1 expression and Foxp3+Helios+ Treg cells was evaluated during HIV-1 infection. Our observations provide new evidence of the roles for Foxp3+Helios+ Treg cells and PD-1 expression on monocyte subsets in HIV pathogenesis.

13.
Nat Commun ; 10(1): 4883, 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31653868

RESUMO

Cellular homeostasis relies on having dedicated and coordinated responses to a variety of stresses. The accumulation of unfolded proteins in the endoplasmic reticulum (ER) is a common stress that triggers a conserved pathway called the unfolded protein response (UPR) that mitigates damage, and dysregulation of UPR underlies several debilitating diseases. Here, we discover that a previously uncharacterized 54-amino acid microprotein PIGBOS regulates UPR. PIGBOS localizes to the mitochondrial outer membrane where it interacts with the ER protein CLCC1 at ER-mitochondria contact sites. Functional studies reveal that the loss of PIGBOS leads to heightened UPR and increased cell death. The characterization of PIGBOS reveals an undiscovered role for a mitochondrial protein, in this case a microprotein, in the regulation of UPR originating in the ER. This study demonstrates microproteins to be an unappreciated class of genes that are critical for inter-organelle communication, homeostasis, and cell survival.

14.
BMC Plant Biol ; 19(1): 454, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31660870

RESUMO

BACKGROUND: Salicylic acid (SA) is a significant signaling molecule that induces rice resistance against pathogen invasion. Protein phosphorylation carries out an important regulatory function in plant defense responses, while the global phosphoproteome changes in rice response to SA-mediated defense response has not been reported. In this study, a comparative phosphoproteomic profiling was conducted by two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) analysis, with two near-isogenic rice cultivars after SA treatment. RESULTS: Thirty-seven phosphoprotein spots were differentially expressed after SA treatment, twenty-nine of which were identified by MALDI-TOF/TOF MS, belonging to nine functional categories. Phosphoproteins involved in photosynthesis, antioxidative enzymes, molecular chaperones were similarly expressed in the two cultivars, suggesting SA might alleviate decreases in plant photosynthesis, regulate the antioxidant defense activities, thus improving basal resistance response in both cultivars. Meanwhile, phosphoproteins related to defense, carbohydrate metabolism, protein synthesis and degradation were differentially expressed, suggesting phosphorylation regulation mediated by SA may coordinate complex cellular activities in the two cultivars. Furthermore, the phosphorylation sites of four identified phosphoproteins were verified by NanoLC-MS/MS, and phosphorylated regulation of three enzymes (cinnamoyl-CoA reductase, phosphoglycerate mutase and ascorbate peroxidase) was validated by activity determination. CONCLUSIONS: Our study suggested that phosphorylation regulation mediated by SA may contribute to the different resistance response of the two cultivars. To our knowledge, this is the first report to measure rice phosphoproteomic changes in response to SA, which provides new insights into molecular mechanisms of SA-induced rice defense.

15.
Immunol Lett ; 214: 37-44, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31473255

RESUMO

Oral tolerance, induced by oral administration of autoantigens, is a promising therapeutic approach to treat type 1 diabetes mellitus (T1DM). However, the degradation of antigens passing through the gastrointestinal tract (GIT) leads to low induction efficiency. Based on our previous study, a single-chain insulin (SCI-59) analog, bound to the surface of lactic acid bacteria (LAB) bacterium-like particles (BLPs), was more stable in the simulated gastric fluid, compared to free SCI-59 and insulin. Based on the analysis of diabetes progression, a significant decrease in the incidence of diabetes was observed in mice fed BLPs-SCI-59. Oral administration of BLPs-SCI-59 can enhance glucose tolerance in NOD mice and this effect may result from the protection of pancreatic islet beta cells, as compared to the free SCI-59 group and BLPs group. Oral administration of BLPs-SCI-59 can significantly reduce insulitis and preserve the ability of insulin secretion in treated mice. Oral vaccination with BLPs-SCI-59 induced SCI-59 specific T cell tolerance in treated mice, which may due to the repair of Th1/Th2 imbalance and increased CD4+CD25+FoxP3+ regulatory T cells (Tregs). These results show that oral vaccination with BLPs-SCI-59 is a promising way to prevent T1DM in NOD mice.

16.
Environ Pollut ; 254(Pt B): 113113, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31484101

RESUMO

Rapid industrialization and urbanization have resulted in widespread pollution of airborne particulate matter (PM) containing various heavy metals with adverse human-health effects. Health risk assessment of PM calls for accurate evaluation of the bioaccessibility, instead of the total content, of heavy metals in PM. Here, we demonstrated that the leachable fraction of particle-bound As, Pb, Cr, Mn, Cd, Cu, Ni and Zn in lung fluid within the typical retention duration of particles in human lungs varied drastically among particles originated from different air pollution sources, including coal combustion, biomass combustion, fugitive dust, road dust, construction dust, cement and soil. Moreover, bioaccessibility of heavy metals, particularly in biomass combustion, cement and soil particles, was strongly dependent on pollution sources, and the particulate Cu, Ni, Pb and Cd appeared to be the primary indicators of the source dependence of heavy metal bioaccessibility. Using total rather than bioaccessible concentrations of particle-bound heavy metals not only led to overestimation of the health risk of source particles, but more importantly, inaccurate identification of the high-risk pollution sources and the priority metal pollutants in the source particles. When considering bioaccessibility of particle-bound heavy metals examined in this study, coal combustion products exhibited the highest carcinogenic and noncarcinogenic risks among all source particles, whereas cement particles would be the source with highest risk based on total metal content. As and Mn appeared to be the main drivers for the noncarcinogenic risks of source particles, while As, Ni and Cr were the major contributors to the carcinogenic risks of source particles, significantly different from those based on total contents. This research underlines the importance of incorporating bioaccessibility into health risk indexes of frequently occurring particle-bound heavy metals from specific air pollution sources, which will facilitate risk-based assessment of source contribution and hence effective source regulation of airborne PM.

17.
Org Lett ; 21(18): 7450-7454, 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31508970

RESUMO

Rare-earth catalysis has become a hot topic in the field of catalytic organic reaction. Chain ethers mostly have lower reactivity and lower boiling points which limited their reaction scope. Herein, we found a rare-earth Y(OTf)3 can catalyze the coupling reaction of ethers especially chain ethers and thioethers with azaarenes. This protocol features simple operations, a broad substrate scope (31 examples), moderate to good yields (up to 85%), and atom economy.

18.
Int J Nanomedicine ; 14: 6061-6071, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31534336

RESUMO

Background: Doxorubicin (DOX), a broad-spectrum chemotherapy drug, is clinically employed to treat cancers especially for breast cancer and lung cancer. But its clinical applications are limited by the dose-dependent cardiac toxicity. Resveratrol (Res), a polyphenolic antitoxin, has been proved to be capable of improving the cardiomyocyte calcium cycling by up-regulating SIRT-1-mediated deacetylation to inhibit DOX-induced cardiotoxicity. Purpose: The objective of this study was to develop a solid lipid nanoparticle (SLN) loaded with Res to trigger inhibition of DOX-induced cardiotoxicity. Methods: Res-SLN was prepared by emulsification-diffusion method followed by sonication and optimized using central composite design/response surface method. The Res-SLN was further evaluated by dynamic light scattering, transmission electron microscopy for morphology and high performance liquid chromatography for drug loading and release profile. And the Res distribution in vivo was determined on rats while the effect of inhibit DOX-induced cardiotoxicity was investigated on mice. Results: Res-SLN with homogeneous particle size of 271.13 nm was successfully formulated and optimized. The prepared Res-SLN showed stable under storage and sustained release profile, improving the poor solubility of Res. Heart rate, ejection fractions and fractional shortening of Res-SLN treating mice were found higher than those on mice with cardiac toxicity induced by single high-dose intraperitoneal injection of DOX. And the degree of myocardial ultrastructural lesions on mice was also observed. Conclusion: Res-SLN has a certain therapeutic effect for protecting the myocardium and reducing DOX-induced cardiotoxicity in mice.

19.
Medicine (Baltimore) ; 98(37): e16945, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31517815

RESUMO

BACKGROUND: Traditional Chinese medicine (TCM) has gradually drawn the attention of clinicians as an alternative choice for insomniacs and TCM yangxin anshen therapy (TYAT), as a crucial therapy of treating insomniacs, is based on the theory of syndrome differentiation. However, owing to the lack of evidence-based medical evidence, the authors intend to carry out this study to evaluate TYAT's effectiveness and safety. METHODS: Seven electronic databases will be searched from inception to July 2019. Two authors will independently identify randomized controlled trials, fetch data and assess the risk of bias with tools provided by Cochrane. A comprehensive meta-analysis will be conducted with the Cochrane Collaboration software (Review Manager 5.3) for eligible and appropriate studies. Further, the evidence will be assessed with the grading of recommendations assessment, development, and evaluation approach. RESULTS: This article will be dedicated to assessing TYAT's efficacy and safety for insomniacs. CONCLUSION: This systematic review and meta-analysis may provide persuasive evidence for the clinical application of TYAT in treating insomnia. TRIAL REGISTRATION NUMBER: PROSPERO CRD 42019135115.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Hipnóticos e Sedativos/uso terapêutico , Metanálise como Assunto , Distúrbios do Início e da Manutenção do Sono/terapia , Revisão Sistemática como Assunto , Medicamentos de Ervas Chinesas/efeitos adversos , Humanos , Hipnóticos e Sedativos/efeitos adversos , Ensaios Clínicos Controlados Aleatórios como Assunto
20.
J Chromatogr Sci ; 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31560746

RESUMO

A combination method of ultra-performance liquid chromatography (UPLC) coupled with diode array detection has been developed for quality evaluation of Qinma prescription (QMP), based on chromatographic fingerprint technology with the similarity analysis (SA) and the quantitative analysis of 12 components by hierarchical cluster analysis (HCA). The established method has been validated by linearity, precision, repeatability, stability and recovery tests. The UPLC fingerprints with 17 common peaks of 5 QMP samples prepared by different extraction methods including water decoction extraction, water extraction-ethanol precipitation method, ethanol reflux extraction, ethanol extraction-water precipitation method and methanol ultrasonic extraction were obtained, and the SA results indicated that similarity index was greatly influenced by the large peak. The similarity index ranged from 0.816 to 0.999 basing on 17 peaks, which has been decreased to 0.683-0.999 basing on 16 peaks without the large peak of baicalin (BA). The results of simultaneous quantification of 12 components in these 5 QMP samples proved that BA, gallic acid (GA), wogonoside (WOG) and gentiopicroside (GEN) were the major ingredients in QMP with high contents >1.44 (mg/g), indicating that ethanol reflux was the most effective extraction method. Integrating fingerprint analysis, simultaneous determination and HCA, the established method is rapid, sensitive, accurate and readily applicable. All the results indicated that the combination method can control the quality of QMP and its related traditional Chinese medicinal compounds more comprehensively and scientifically.

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