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1.
Methods Mol Biol ; 2375: 77-90, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34591300

RESUMO

Human tissue-engineered blood vessels (TEBVs) that exhibit vasoactivity can be used to test drug toxicity, modulate pro-inflammatory cytokines, and model disease states in vitro. We developed a novel device to fabricate arteriole-scale human endothelialized TEBVs in situ with smaller volumes and higher throughput than previously reported. Both primary and induced pluripotent stem cell (iPSC)-derived cells can be used. Four collagen TEBVs with 600µm inner diameter and 2.9 mm outer diameter are fabricated by pipetting a solution of collagen and medial cells into a three-layer acrylic mold. After gelation, the TEBVs are released from the mold and dehydrated. After suturing the TEBVs in place and changing the mold parts to form a perfusion chamber, the TEBVs are endothelialized in situ, and then media is perfused through the lumen. By removing 90% of the water after gelation, the TEBVs become mechanically strong enough for perfusion at the physiological shear stress of 0.4 Pa within 24 h of fabrication and maintain function for at least 5 weeks.

2.
J Hazard Mater ; 421: 126810, 2022 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-34365231

RESUMO

The pollution of perfluorooctanoic acid (PFOA) in water bodies has been a serious threat to environment and human health. Ordered mesoporous carbons (OMCs) with different oxygen contents were prepared and first used for adsorbing PFOA from aqueous solutions. The OMC-900 with a lower oxygen content has a higher PFOA adsorption capacity than the oxygen-rich OMC-700. OMCs require a much shorter time to reach the adsorption equilibrium comparing with other adsorbents reported in literature. The mesopores play an important role in this rapid adsorption kinetics. The pseudo-second-order model better fitted the kinetic data. The multilayers adsorption was proposed for the adsorption of PFOA onto OMCs since the Freundlich isotherm model fits the experimental data well. The micelle or hemi-micelle structures may be formed during the adsorption. Various background salts showed a positive effect on PFOA adsorption due to the salting-out and divalent bridge effects. The humic acid can lead to a discernible reduction in PFOA adsorption by competing for adsorption sites on OMCs. The hydrophobic interaction and electrostatic interaction adsorption mechanisms were proposed and verified by the adsorption data. The high adsorption capacity and fast adsorption kinetics of the OMC make it a potential adsorbent for PFOA removal in engineering applications.


Assuntos
Carbono , Fluorcarbonetos , Adsorção , Caprilatos , Humanos , Cinética , Oxigênio
3.
J Colloid Interface Sci ; 606(Pt 2): 1120-1127, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34487932

RESUMO

Layered double hydroxides (LDHs) have been considered as favorable pseudocapacitive electrode materials for supercapacitors due to their tunable layered structure/compositions and low cost. Here, we report the NiCo-LDH hollow spheres prepared with Co-glycerate as the sacrificial template and cobalt source. The hollow spheres are assembled with frizzy NiCo-LDH nanosheets, where the hollow structure can inhibit agglomeration of the LDH nanosheets to expose more active sites and shorten the diffusion path of electrolyte ions. The prepared NiCo-LDH hollow spheres show a high specific capacitance of 1962 F g-1 at 1 A g-1 and good capacitance retention rate of 66.4 % at 30 A g-1. The asymmetric supercapacitors fabricated using NiCo-LDH hollow spheres as positive electrode yields a large energy density 62.9 Wh kg-1 at the power density of 0.8 kW kg-1. This research may develop a facile synthesis way to prepare LDH hollow spheres for supercapacitors.


Assuntos
Cobalto , Hidróxidos , Capacitância Elétrica , Eletrodos
4.
J Hazard Mater ; 421: 126770, 2022 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-34358975

RESUMO

Ubiquitous nanoplastics (NPs) increase exposure risks to humans through the food chain and/or other ways. However, huge knowledge gaps exist regarding the fate and adverse impact of NPs on the human cardiovascular system. Autophagy is an important catabolic pathway that disposes of cytoplasmic waste through the lysosomes. In this study, we pursued to determine the interaction and autophagy effect of polystyrene nanoplastics (PS-NPs) (100 and 500 nm in size) on human umbilical vein endothelial cells (HUVECs). The results showed both sizes of PS-NPs interacted with almost all the treated HUVECs in a time- and concentration-dependent manner, and 500 nm PS-NPs were only bound to the surface of cell membranes, whereas 100 nm PS-NPs were taken up by HUVECs and aggregated in the cytoplasm. Furthermore, exposure to 25 µg/mL of 500 nm PS-NPs for 48 h significantly increased lactate dehydrogenase release from HUVECs, while internalized 100 nm PS-NPs not only caused cell membrane damage, but also induced autophagy initiation and autophagosome formation. By a mCherry-GFP-LC3 lentivirus infection assay, we also demonstrated that autophagic flux level was impaired in response to 100 nm PS-NPs. Herein, our results provide new insight into the size-dependent internalization and autophagy response to PS-NPs in HUVECs.


Assuntos
Nanopartículas , Poliestirenos , Autofagia , Células Endoteliais da Veia Umbilical Humana , Humanos , Lisossomos , Microplásticos , Nanopartículas/toxicidade
5.
Chemosphere ; 287(Pt 1): 132046, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34474386

RESUMO

Phthalate is an environmental endocrine disruptor that causes direct and intergenerational male reproductive damage. However, its mechanisms require further investigation. The role of gut microbiota in male reproductive function has been gradually revealed in the past. To explore the intergenerational testicular injury and the influence on offspring gut microbiota of the widely used phthalate dibutyl phthalate (DBP), we conducted a prenatal DBP exposure experiment with microbiota sequencing. We finally explained the gestational DBP exposure-induced gut dysbacteriosis, which is one of the mechanisms of testicular injury in the offspring. The occurrence of seminiferous atrophy and spermatogenic cells apoptosis showed a slight increase. Our study partially supported the results of previous research works on the characteristics of gut dysbacteriosis, which featured the increased relative abundance of Bacteroidetes, Prevotella and P. copri. Focusing on the role of gut microbiota in reproductive function is important. Future studies need to investigate the relationship between environmental pollution and human health.


Assuntos
Disruptores Endócrinos , Microbioma Gastrointestinal , Dibutilftalato/toxicidade , Disruptores Endócrinos/toxicidade , Feminino , Humanos , Masculino , Gravidez , Reprodução , Testículo
6.
Sci Total Environ ; 805: 150231, 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-34530352

RESUMO

Coal is not only the main fossil fuel in China but also a pollution source. To evaluate the impact of coal production on the environment, a life cycle assessment (LCA) was conducted on the mining process of a typical coal mine in China by using the SimaPro 9.0.0 software. The Ecoinvent v3 database was used to provide the background data, and midpoint results with uncertainty information were calculated using the ReCiPe Midpoint (H) method. After normalising the midpoint results, fossil depletion was identified as the most predominant environmental impact category, followed by marine ecotoxicity, freshwater ecotoxicity, climate change, freshwater eutrophication, and human toxicity. The contribution analysis indicates that coal mining activities, consumption of steel and electricity, and mine ventilation are the key processes causing the above-mentioned environmental impact categories, which should be paid special attention. According to the sensitivity analysis, the primary countermeasures for addressing the environmental issues are to reduce the mining activities and improve the efficiency of coal mining and utilisation. In addition, the quantitative and comparative analyses show that the gas extraction production mode is beneficial to the environment. Finally, technical measures were proposed to promote green and sustainable development of the coal industry. This research can provide guidance for ensuring national energy security and promoting healthy development of the national economy.


Assuntos
Minas de Carvão , Animais , China , Carvão Mineral/análise , Poluição Ambiental , Eutrofização , Humanos , Estágios do Ciclo de Vida
7.
Ocean Coast Manag ; 216: 105975, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34840439

RESUMO

The COVID-19 pandemic has affected China's economic development, international exchanges and other aspects to varying degrees, among which the impact on China's fishery development should not be underestimated. The fishery is a traditional and vast industry in China. The ongoing COVID-19 outbreak has also exposed potential problems in China's fishery development, such as the lack of specialised aquatic product quality and safety supervision and testing institutions. New problems have also arisen, such as the decline in the market demand for fish and aquatic products, affecting fishers' income, livelihood and so on. These deficiencies require China to establish a matching legal system to correspondingly solve the current legal and political problems. This paper will systematically discuss the impact of the COVID-19 on China's fisheries sector from the perspectives of 'fishers' and 'aquatic products' after the outbreak of the COVID-19 pandemic and analyse how to deal with various problems by referring to relevant experience in light of China's national conditions and characteristics.

8.
Nature ; 600(7887): E1-E3, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34853450
9.
Int J Gen Med ; 14: 8611-8620, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34849006

RESUMO

Background: Gliomas are prevalent primary intracerebral malignant tumors. Increasing evidence indicates an association between the immune signature and Grade II/III glioma prognosis. Thus, we aimed to develop an immune-related gene pair (IRGP) signature that can be used as a prognostic tool in Grade II/III glioma. Methods: The gene expression levels and clinical information of Grade II/III glioma patients were collected from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) databases. The TCGA data were randomly divided into a training cohort (n = 249) and a validation cohort (n = 162), and a CGGA dataset served as an external validation group (n = 605). IRGPs significantly associated with prognosis were selected by Cox regression. Gene set enrichment analysis and filtration were performed with the IRGPs. Results: Within a set of 1991 immune genes, 8 IRGPs including 15 unique genes that significantly affect survival constituted a gene signature. In the validation datasets, the IRGP signature significantly stratified patients with Grade II/III glioma into low- and high-risk groups (P < 0.001), and the IRGP index was found to be an independent prognostic factor through univariate and multivariate analyses (P < 0.05). Additionally, 26 functional pathways were identified through the intersection of Gene Set Enrichment Analysis (GSEA) and Gene Ontology (GO) enrichment analysis. Conclusion: The IRGP signature demonstrated good prognostic value for Grade II/III gliomas, which may provide new insights into individual treatment for glioma patients. The IRGPs might function through the identified 26 functional pathways.

10.
Sci Rep ; 11(1): 23356, 2021 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-34857818

RESUMO

PCBP1 is a multifunctional RNA-binding protein (RBP) expressed in most human cells and is involved in posttranscriptional gene regulation. PCBP1 regulates the alternative splicing, translation and RNA stability of many cancer-related genes and has been identified as a potential tumour suppressor gene. PCBP1 inhibits the invasion of hepatocellular carcinoma (HCC) cells, but there are few studies on the specific regulatory target and mechanism of RBPs in HCC, and it is unclear whether PCBP1 plays a role in tumour metastasis as a splicing factor. We analysed the regulation of gene expression by PCBP1 at the transcriptional level. We obtained and analysed PCBP1-knockdown RNA-seq data and eCLIP-seq data of PCBP1 in HepG2 cells and found that PCBP1 widely regulates the alternative splicing and expression of genes enriched in cancer-related pathways, including extracellular matrix, cell adhesion, small molecule metabolic process and apoptosis. We validated five regulated alternative splicing events affected by PCBP1 using RT-qPCR and found that there was a significant difference in the expression of APOC1 and SPHK1 between tumour and normal tissues. In this study, we provided convincing evidence that human PCBP1 profoundly regulates the splicing of genes associated with tumour metastasis. These findings provide new insight into potential markers or therapeutic targets for HCC treatment.

11.
Front Med (Lausanne) ; 8: 735318, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34859005

RESUMO

Purpose: To evaluate the treatment solutions and effectiveness of intravitreal ranibizumab (RBZ) or conbercept in patients with wet age-related macular degeneration (wAMD) in a real-life setting in China. Methods: The medical records of 368 patients with wAMD who started RBZ or conbercept treatment between 1 May 2014 and 30 April 2018 were evaluated. All patients were defined on fundus angiography at baseline to determine the subtype of AMD (PCV or CNV). We report visual acuity (VA) and central retinal thickness (CRT) measurements at baseline and 12 months. Results: The average number of anti-VEGF injections was 2.1 ± 1.2. The BCVA improvement of these two groups was similar with a difference of 1.00 letter (95% CI: -1.4~3.4, p = 0.8505). At the end of the study, a BCVA increase of at least 5 letters was determined to be a satisfactory efficacy endpoint. Several factors were related to the possible improvement in the satisfactory efficacy endpoint, including female sex (OR 2.07, 95% CI 1.22~3.51), number of injections (OR 1.40, 95% CI 1.12~1.75) and VA change at the first month (OR 13.75, 95% CI 7.41~25.51). Additionally, some factors were related to the possible reduction in the satisfactory efficacy endpoint, including diabetes (OR 0.27, 95% CI 0.10~0.73) and disease history (OR 0.75, 95% CI 0.57~0.98). Conclusion: Our study demonstrates that anti-VEGF drugs can effectively improve BCVA and reduce CRT in AMD patients. Sex, number of injections, VA change at the first month, diabetes and disease history are the most important factors affecting visual acuity.

12.
ACS Nano ; 2021 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-34860017

RESUMO

In order to overcome the shuttling effect of soluble polysulfides in lithium-sulfur (Li-S) batteries, we have designed and synthesized a creative MoS2-MoO3/carbon shell (MoS2-MoO3/CS) composite by a H2O2-enabled oxidizing process under mild conditions, which is further used for separator modification. The MoS2-MoO3 heterostructures can conform to the CS morphology, forming two-dimensional nanosheets, and thus shorten the transport path of lithium ion and electrons. Based on our theoretical calculations and experiments, the heterostructures show strong surface affinity toward polysulfides and good catalytic activity to accelerate polysulfide conversion. Benefiting from the above merits, the Li-S battery with a MoS2-MoO3/CS modified separator exhibits good electrochemical performance: it delivers a high discharge capacity of 1531 mAh g-1 at 0.2 C; the initial capacity can be maintained by 92% after 600 cycles at 1 C, and the discharge capacity decay rate is only 0.0135% per cycle. Moreover, the MoS2-MoO3/CS battery still achieves good cycling stability with 78% capacity retention after 100 cycles at 0.2 C with a high sulfur loading of 5.9 mg cm-2. This work offers a facile design to construct the MoS2-MoO3 heterostructures for high-performance Li-S batteries, and may also improve one's understanding on the heterostructure contribution during polysulfide adsorption and conversion.

13.
Exp Hematol Oncol ; 10(1): 52, 2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34758872

RESUMO

BACKGROUND: MET amplification plays an important role in the development of non-small-cell lung cancer (NSCLC) either de novo or in resistance to epidermal growth factor receptor tyrosine-kinase inhibitor (EGFR-TKI) settings. Fluorescence in situ hybridization (FISH) is the standard method for MET amplification. With more and more discoveries of oncogenic driver genes, next-generation sequencing (NGS) plays a significant role in precision oncology. Meanwhile, the role of NGS in MET amplification remains uncertain. METHODS: Forty patients diagnosed with advanced NSCLC were included. FISH and NGS were conducted prior to MET inhibitors treatment. MET amplification by FISH was defined as a MET/CEP7 ratio of > 2.0 and/or copy number (CN) > 5. MET amplification by NGS was defined as gene copy number (GCN) ≥ 5. RESULTS: The concordance rate among FISH and NGS was 62.5% (25/40). MET amplification identified by FISH showed the optimal predictive value. The partial response (PR) rate was 68.0% (17/25 with MET amplification) vs. 6.7% (1/15 without MET amplification); the median progression-free survival (PFS) was 5.4 months versus 1.0 months (P < 0.001). MET amplification identified by NGS failed to distinguish significant clinical outcomes. The PR rate was 60.0% (6/10, with MET GCN ≥ 5) vs. 40.0% (12/30, with MET GCN < 5); the median PFS was 4.8 months vs. 2.2 months (P = 0.357). The PR rate was 68.8% (11/16) and the median PFS was 4.8 months in patients with focal amplification by NGS. CONCLUSIONS: MET amplification identified by FISH remains the optimal biomarker to identify suitable candidates for MET-TKI therapy. In comparison, amplification identified by NGS seems not as robust to be effective predictive biomarker. Further exploration is needed regarding the focal amplification by NGS in predicting the efficacy.

14.
Front Immunol ; 12: 686809, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34777331

RESUMO

Immune microenvironment of prostate cancer (PCa) is implicated in disease progression. However, previous studies have not fully explored PCa immune microenvironment. This study used ssGSEA algorithm to explore expression levels of 53 immune terms in a combined PCa cohort (eight cohorts; 1,597 samples). The top 10 immune terms were selected based on the random forest analysis and used for immune-related risk score (IRS) calculation. Furthermore, we explored differences in clinical and genomic features between high and low IRS groups. An IRS signature based on the 10 immune terms showed high prediction potential for PCa prognosis. Patients in the high IRS group showed significantly higher percentage of immunotherapy response factors, implying that IRS is effective in predicting immunotherapy response rate. Furthermore, consensus clustering was performed to separate the population into three IRSclusters with different clinical outcomes. Patients in IRScluster3 showed the worst prognosis and highest immunotherapy response rate. On the other hand, patients in IRScluster2 showed better prognosis and low immunotherapy response rate. In addition, VGLL3, ANPEP, CD38, CCK, DPYS, CST2, COMP, CRISP3, NKAIN1, and F5 genes were differentially expressed in the three IRSclusters. Furthermore, CMap analysis showed that five compounds targeted IRS signature, thioridazine, trifluoperazine, 0175029-0000, trichostatin A, and fluphenazine. In summary, immune characteristics of PCa tumor microenvironment was explored and an IRS signature was constructed based on 10 immune terms. Analysis showed that this signature is a useful tool for prognosis and prediction of immunotherapy response rate of PCa.

15.
Adv Mater ; : e2105410, 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34787336

RESUMO

Noble-metal-free, durable and high-efficiency electrocatalysts for oxygen reduction and evolution reaction (ORR/OER) are vital for rechargeable Zn-air batteries (ZABs). Herein, a flexible and free-standing carbon fiber membrane immobilized with atomically dispersed Fe-N4 /C catalysts (Fe/SNCFs-NH3 ) is synthesized and used as air cathode for ZABs. The intertwined carbon fibers with hierarchical nanopores facilitate the gas transportation, electrolyte infiltration and electron transfer. The larger specific surface area after NH3 activation exposes a high concentration of Fe-N4 /C sites that embedded in the carbon matrix. Modulation of local atomic configurations by sulfur doping in Fe/SNCFs-NH3 catalyst leads to excellent ORR and enhanced OER activities. As-synthesized Fe/SNCFs-NH3 catalyst demonstrates a positive half-wave potential of 0.89 V and a small Tafel slope of 70.82 mV dec-1 , outperforming the commercial Pt/C (0.86 V/94.74 mV dec-1 ) and most reported M-Nx /C (M = Fe, Co, Ni) catalysts. Experimental characterizations and theoretical calculations uncover the crucial role of S doping in regulating ORR and OER activity. The liquid-state ZABs with Fe/SNCFs-NH3 catalyst as air cathode deliver a large peak power density of 255.84 mW cm-2 and a long-term cycle durability over 1000 h. Solid-state ZAB shows stable cycling at various flat/bent/flat states, demonstrating great prospects in portable and wearable device applications. This article is protected by copyright. All rights reserved.

16.
Oncogene ; 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34782718

RESUMO

Bladder cancer (BLCA) is the most common malignant tumor of the urinary system and is characterized by high metastatic rates and poor prognosis. The expression of tight junction protein 1 (TJP1) is associated with bladder cancer invasion; however, the mechanism by which TJP1 affects vasculature remodeling remains unknown. In this study, we found that TJP1 expression correlated with tumor angiogenesis and poor overall survival in clinical samples. Furthermore, TJP1 overexpression promoted tumor angiogenesis in BLCA cells and stimulated recruitment of macrophages to tumors by upregulating CCL2 expression. Mechanistically, TJP1 interacted with TWIST1 and enhanced the transcriptional activity of CCL2. The impairment of tumor angiogenesis caused by knockdown of TJP1 was dramatically rescued by overexpression of TWIST1. Furthermore, TJP1 recruited USP2, which deubiquitinated TWIST1, thereby protecting TWIST1 from proteasome-mediated protein degradation. In conclusion, our results suggest that TJP1 controls angiogenesis in BLCA via TWIST1-dependent regulation of CCL2. We demonstrate that TJP1 functions as a scaffold for the interaction between USP2 and TWIST1 and this may provide potential therapeutic targets in bladder cancer.

17.
Small ; : e2105194, 2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34783451

RESUMO

Annihilation of vacancy clusters in monolayer molybdenum diselenide (MoSe2 ) under electron beam irradiation is reported. In situ high-resolution transmission electron microscopy observation reveals that the annihilation is achieved by diffusion of vacancies to the free edge near the vacancy clusters. Monte Carlo simulations confirm that it is energetically favorable for the vacancies to locate at the free edge. By computing the minimum energy path for the annihilation of one vacancy cluster as a case study, it is further shown that electron beam irradiation and pre-stress in the suspended MoSe2 monolayer are necessary for the vacancies to overcome the energy barriers for diffusion. The findings suggest a new mechanism of vacancy healing in 2D materials and broaden the capability of electron beam for defect engineering of 2D materials, a promising way of tuning their properties for engineering applications.

18.
Nucl Med Commun ; 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34783717

RESUMO

OBJECTIVE: T cell lymphomas are associated with an aggressive worse prognosis. This study is designed to assess T cell lymphomas using 18F-FDG PET/CT. METHODS: Sixty-four patients with newly diagnosed T cell lymphomas underwent PET/computed tomography (PET/CT) scans, 47 cases who were fully followed up were retrospectively reviewed and analyzed. Overall survival (OS) and progression-free survival (PFS) were recorded for prognosis. We measured the maximum standardized uptake value (SUVmax) in all cases, analyzed the correlation between SUVmax and survival and other clinicopathologic parameters. Kaplan-Meier log-rank tests were then used to compare the survival of high and low PET/CT parameter groups, and multivariate Cox proportional hazards regression analysis was carried out to identify predictors of OS and PFS. RESULTS: With a median follow-up of 26.5 (range 0.7-117.5) months, the 1-, 2- and 3-year OS were 75.6, 61.7 and 49.2%, and PFS were 49.3, 39.9 and 29.9%, respectively in 47 patients. Among them, 33 cases progressed with a median time of 9.5 (0.7-115.0) months, and 26 patients died with a median survival time of 26.5 (0.7-117.5) months. Multivariate analysis showed the following independent prognostic factors for OS: age >60 years (P = 0.002), SUVmax >9.7 (P = 0.009) and extranodal involvement of more than one site (P = 0.018). In addition, lactate dehydrogenase level (P = 0.003) and B symptoms (P = 0.018) were independent risk factors for PFS. CONCLUSION: Pretherapy SUVmax may serve as an independent predictor of outcome in patients with newly diagnosed T cell lymphomas.

19.
Nanoscale ; 13(44): 18695-18701, 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34738607

RESUMO

Tandem catalysts with multifunctional sites can achieve high-efficiency catalytic transformations for quickly converting simple raw materials into complex value-added products. The integration of highly active species of metal nanoparticles (NPs) and single-atom catalytic sites (SACs) into one tandem system promises to synthesize an ideal bifunctional catalyst on account of the synergistic effect between NPs and SACs. However, such ideas face some challenges as deactivation or loss of active species, and low efficiency or side reactions caused by the disorder of different active species. Herein, a double-shell microencapsulated nanoreactor was fabricated as a bifunctional catalyst for the one-pot synthesis of cyclic carbonates from olefins. The microcapsules consist of an inner shell of nitrogen-doped porous carbon rich in Zn SACs, an outer shell of mesoporous SiO2, and Au NPs confined between the outer and inner shells, noted as Zn-N-C/Au@mSiO2. Particularly, two active species are spatially compartmented within microcapsules. Furthermore, the catalyst was applied in the one-pot synthesis of styrene carbonate from styrene with CO2 under normal pressure and showed admirable performance. The yield of cyclic carbonate reached 92.9% at 93.2% olefins conversion. Furthermore, the catalyst shows good reusability with little loss of catalytic performance (4.0%) even after using it 15 consecutive times. The unique structure used in this work can rationally integrate diverse catalytic species into one system and offering adequate protection, which provides an effective strategy for the development of multi-site catalysts.

20.
Ann Transl Med ; 9(20): 1516, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34790722

RESUMO

Background: Few studies have exclusively investigated the value of pathological complete response (pCR), in esophageal squamous cell carcinoma (ESCC) patients, although it is a clinically significant parameter to evaluate the impact of neoadjuvant chemoradiotherapy (nCRT) on treatment outcome after surgery. The aim of our study was to explore the relationship between pCR after nCRT and survival among patients with local ESCC. Methods: All patients receiving nCRT followed by surgery in NEOCRTEC5010-trial (NCT01216527) were included. Non-pCR patients were classified into three subgroups: ypTanyN0M0, ypT0NanyM0 and ypTanyNanyM0. The Kaplan-Meier method with log-rank test was employed to evaluate disease-free survival (DFS) and overall survival (OS). Multivariate regression analysis was performed using a Cox proportional hazards model to identify clinicopathological parameters associated with pCR. Results: Among the 185 patients included, 80 (43.2%) achieved pCR after nCRT. The mean survival time of the pCR group was significantly longer than that of the non-pCR group (92.6 vs. 69.2 months; HR, 2.70; 95% CI: 1.48-4.92; P=0.001). The 5-year OS and DFS of the pCR group were 79.3% and 77% respectively, compared to 54.8% and 51.2%, respectively, in the non-pCR group. The results showed that the OS and DFS of the ypTanyN0M0 group were better than those of the ypT0NanyM0 group and the ypTanyNanyM0 group. We also found that the number of dissected lymph nodes and pCR were independent risk factors for DFS and OS rates. Conclusions: pCR after nCRT is an important prognostic indicator of OS and DFS in patients with ESCC. In addition, lymph-node status could represent an important parameter in the prognostic evaluation of esophageal cancer patients.

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