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1.
Int Immunopharmacol ; 81: 106176, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32044667

RESUMO

A high salt diet (HSD) is among the most important risk factors for many diseases. One mechanism by which HSD aggravates cerebral ischemic injury is independent of blood pressure changes. The direct role of HSD in inflammation after cerebral ischemia is unclear. In this research, after twenty-one days of being fed a high salt diet, permanent focal ischemia was induced in mice via operation. At 12 h and 1, 3 and 5 days postischemia, the effects of HSD on the lesion volume, microglia polarization, aldose reductase (AR) expression, and inflammatory processes were analyzed. We report that in mice, surplus dietary salt promotes inflammation and increases the activation of classical lipopolysaccharide (LPS)-induced microglia/macrophages (M1). This effect depends on the expression of the AR protein in activated microglia after permanent middle cerebral artery ligation (pMCAL) in HSD mice. The administration of either the AR inhibitor Epalrestat or a p38-neutralizing antibody blocked the polarization of microglia and alleviated stroke injury. In conclusion, HSD promotes polarization in pro-inflammatory M1 microglia by upregulating the expression of the AR protein via p38/MAPK, thereby exacerbating the development of ischemia stroke.

2.
Shock ; 53(3): 284-292, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32045395

RESUMO

BACKGROUND: Sepsis is a potentially life-threatening complication of an underlying infection that quickly triggers tissue damage in multiple organ systems. To date, there are no established useful prognostic biomarkers for sepsis survival prediction. Sphingosine-1-phosphate (S1P) and its receptor S1P receptor 1 (S1PR1) are potential therapeutic targets and biomarkers for sepsis, as both are active regulators of sepsis-relevant signaling events. However, the identification of an S1PR1-related gene signature for prediction of survival in sepsis patients has yet to be identified. This study aims to find S1PR1-associated biomarkers which could predict the survival of patients with sepsis using gene expression profiles of peripheral blood to be used as potential prognostic and diagnostic tools. METHODS: Gene expression analysis from sepsis patients enrolled in published datasets from Gene Expression Omnibus was utilized to identify both S1PR1-related genes (co-expression genes or functional-related genes) and sepsis survival-related genes. RESULTS: We identified 62-gene and 16-gene S1PR1-related molecular signatures (SMS) associated with survival of patients with sepsis in discovery cohort. Both SMS genes are significantly enriched in multiple key immunity-related pathways that are known to play critical roles in sepsis development. Meanwhile, the SMS performs well in a validation cohort containing sepsis patients. We further confirmed our SMSs, as newly developed gene signatures, perform significantly better than random gene signatures with the same gene size, in sepsis survival prognosis. CONCLUSIONS: Our results have confirmed the significant involvement of S1PR1-dependent genes in the development of sepsis and provided new gene signatures for predicting survival of sepsis patients.

3.
Phys Chem Chem Phys ; 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32031546

RESUMO

LiBH4 is one of the most promising solid electrolyte materials for use in solid-state batteries because its hexagonal phase above 110 °C offers Li-ion conductivity of almost 10-3 S cm-1. However, near room temperature, its orthorhombic phase delivers Li-ion conductivity of only 10-8 S cm-1, which considerably hampers its further applications. In the present study, a highly disordered interface between LiBH4 and two-dimensional MoS2 in the composite material was formed, yielding ionic conductivity of 10-4 S cm-1 at room temperature. LiBH4 and MoS2 are found to be in close contact without the formation of any intermediate phase at the interface. First-principles calculations employing density functional theory (DFT) and the nudged elastic band (NEB) method reveal that the migration energy barrier on three specific pathways could be established via microstructure analyses. It was found that the interface between the two phases yields the lowest Li-ion diffusion barrier among all the possible Li-ion pathways; further, the superior conductivity of the composite could be attributed to the interface with high Li-ion conductivity. This study proposes a new strategy for designing solid electrolytes and provides certain possibilities for two-dimensional materials to serve as superior solid electrolytes.

4.
Viruses ; 12(2)2020 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-32050494

RESUMO

New strategies against antibiotic-resistant bacterial pathogens are urgently needed but are not within reach. Here, we present in vitro and in vivo antimicrobial activity of TSPphg, a novel phage lysin identified from extremophilic Thermus phage TSP4 by sequencing its whole genome. By breaking down the bacterial cells, TSPphg is able to cause bacteria destruction and has shown bactericidal activity against both Gram-negative and Gram-positive pathogenic bacteria, especially antibiotic-resistant strains of Klebsiella pneumoniae, in which the complete elimination and highest reduction in bacterial counts by greater than 6 logs were observed upon 50 µg/mL TSPphg treatment at 37 °C for 1 h. A murine skin infection model further confirmed the in vivo efficacy of TSPphg in removing a highly dangerous and multidrug-resistant Staphylococcus aureus from skin damage and in accelerating wound closure. Together, our findings may offer a therapeutic alternative to help fight bacterial infections in the current age of mounting antibiotic resistance, and to shed light on bacteriophage-based strategies to develop novel anti-infectives.

5.
Sci Total Environ ; 711: 135183, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32000350

RESUMO

China is the largest rice producer and consumer in the world. Accurate estimations of paddy rice planting area and rice grain production is important for feeding the increasing population in China. However, Southern China had substantial losses in paddy rice area over the last three decades in those regions where paddy rice has traditionally been produced. Several studies have shown increased paddy rice area in Northeast China. Here we document the annual dynamics of paddy rice area, gross primary production (GPP), and grain production in Northeast China (Heilongjiang, Jilin and Liaoning provinces) during 2000-2017 using agricultural statistical data, satellite images, and model simulations. Annual maps derived from satellite images show that paddy rice area in Northeast China has increased by 3.68 million ha from 2000 to 2017, which is more than the total combined paddy rice area of North Korea, South Korea, and Japan. Approximately 82% of paddy rice pixels had an increase in annual GPP during 2000-2017. The expansion of paddy rice area slowed down substantially since 2015. Annual GPP from those paddy rice fields cultivated continuously over the 18 years were moderately higher than that from other paddy rice fields, which suggested that improved management practices could increase grain production in the region. There was a strong linear relationship between annual GPP and annual rice grain production in Northeast China by province and year, which illustrates the potential of using satellite-based data-driven model to track and assess grain production of paddy rice in the region. Northeast China is clearly an emerging rice production base and plays an increasing role in crop production and food security in China. However, many challenges for the further expansion and sustainable cultivation of paddy rice in Northeast China remain.

6.
Artigo em Inglês | MEDLINE | ID: mdl-31933057

RESUMO

The goals of this study were to increase the production of antroquinonol (AQ) and to elucidate the response mechanism of the cell membrane during the in situ extractive fermentation (ISEF) of Antrodia camphorata S-29. Through ISEF, the concentration of AQ reached a maximum of 146.1 ± 2.8 mg/L, which was approximately (7.4 ± 0.1)-fold that of the control (coenzyme Q0-induced fermentation). Transcriptome sequencing showed that four genes (FAD2, fabG, SCD, and FAS1) related to fatty acid biosynthesis were upregulated. FAD2 and SCD may regulate the increase in oleic acid (C18:1) and linoleic acid (C18:2) in the cell membrane of A. camphorata S-29, resulting in an increase in cell membrane permeability. AQ was successfully transferred to the n-tetradecane phase through the cell membrane, reducing product feedback inhibition and improving the production of AQ from A. camphorata S-29.

7.
Proc Natl Acad Sci U S A ; 117(5): 2473-2483, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-31941714

RESUMO

Neddylation is a ubiquitination-like pathway that controls cell survival and proliferation by covalently conjugating NEDD8 to lysines in specific substrate proteins. However, the physiological role of neddylation in mammalian metabolism remains elusive, and no mitochondrial targets have been identified. Here, we report that mouse models with liver-specific deficiency of NEDD8 or ubiquitin-like modifier activating enzyme 3 (UBA3), the catalytic subunit of the NEDD8-activating enzyme, exhibit neonatal death with spontaneous fatty liver as well as hepatic cellular senescence. In particular, liver-specific UBA3 deficiency leads to systemic abnormalities similar to glutaric aciduria type II (GA-II), a rare autosomal recessive inherited fatty acid oxidation disorder resulting from defects in mitochondrial electron transfer flavoproteins (ETFs: ETFA and ETFB) or the corresponding ubiquinone oxidoreductase. Neddylation inhibition by various strategies results in decreased protein levels of ETFs in neonatal livers and embryonic hepatocytes. Hepatic neddylation also enhances ETF expression in adult mice and prevents fasting-induced steatosis and mortality. Interestingly, neddylation is active in hepatic mitochondria. ETFs are neddylation substrates, and neddylation stabilizes ETFs by inhibiting their ubiquitination and degradation. Moreover, certain mutations of ETFs found in GA-II patients hinder the neddylation of these substrates. Taken together, our results reveal substrates for neddylation and add insight into GA-II.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31953901

RESUMO

Transition metal-catalyzed direct asymmetric reductive amination (ARA) of diaryl or sterically hindered ketones remains unknown and an open challenge. Herein, Ru-catalyzed direct asymmetric reductive amination of ortho -OH-substituted diaryl and sterically hindered ketones with ammonium salts has been disclosed for the first time, offering a straightforward route toward the synthesis of synthetically useful chiral primary diarylmethylamines and sterically hindered benzylamines (up to 97% yield, 93->99% ee). The elaborations of chiral amine products into bioactive compounds have been exhibited through manipulations on the removable and convertible -OH group. Moreover, gram-scale synthesis of chiral sterically bulky amine 6ac and its application in the synthesis of a new benzo-bisoxazine ligand further showcase the utility of this method.

9.
Cancer Med ; 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31953923

RESUMO

Long non-coding RNA (lncRNA) is emerging as a pivotal regulator in tumorigenesis and aggressive progression. Here, we focused on an oncogenic lncRNA, ARAP1 antisense RNA 1 (ARAP1-AS1), which was notably upregulated in cervical cancer (CC) tissues, cell lines and serum. High ARAP1-AS1 expression was closely associated with larger tumor size, advanced FIGO stage as well as lymph node metastasis. Importantly, it was identified as an effective diagnostic and prognostic biomarker for CC. In vitro and in vivo assays showed that knockdown of ARAP1-AS1 inhibited, while overexpression of ARAP1-AS1 promoted CC cell growth and dissemination. Stepwise mechanistic dissection unveiled that ARAP1-AS1 could directly interact with PSF to release PTB, resulting in accelerating the internal ribosome entry site (IRES)-driven translation of proto-oncogene c-Myc, thereby facilitating CC development and progression. Moreover, c-Myc was able to transcriptionally activate ARAP1-AS1 by directly binding to the E-box motif located on ARAP1-AS1 promoter. Taken together, our findings clearly reveal the crucial role of ARAP1-AS1 in CC tumorigenesis and metastasis via regulation of c-Myc translation, targeting ARAP1-AS1 and its related regulatory loop implicates the therapeutic possibility for CC patients.

10.
J Hematol Oncol ; 13(1): 5, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31915027

RESUMO

BACKGROUND: Long non-coding RNAs (lncRNAs) possess significant regulatory functions in multiple biological and pathological processes, especially in cancer. Dysregulated lncRNAs in hepatocellular carcinoma (HCC) and their therapeutic applications remain unclear. METHODS: Differentially expressed lncRNA profile in HCC was constructed using TCGA data. LINC00958 expression level was examined in HCC cell lines and tissues. Univariate and multivariate analyses were performed to demonstrate the prognostic value of LINC00958. Loss-of-function and gain-of-function experiments were used to assess the effects of LINC00958 on cell proliferation, motility, and lipogenesis. Patient-derived xenograft model was established for in vivo experiments. RNA immunoprecipitation, dual luciferase reporter, biotin-labeled miRNA pull-down, fluorescence in situ hybridization, and RNA sequencing assays were performed to elucidate the underlying molecular mechanisms. We developed a PLGA-based nanoplatform encapsulating LINC00958 siRNA and evaluated its superiority for systemic administration. RESULTS: We identified a lipogenesis-related lncRNA, LINC00958, whose expression was upregulated in HCC cell lines and tissues. High LINC00958 level independently predicted poor overall survival. Functional assays showed that LINC00958 aggravated HCC malignant phenotypes in vitro and in vivo. Mechanistically, LINC00958 sponged miR-3619-5p to upregulate hepatoma-derived growth factor (HDGF) expression, thereby facilitating HCC lipogenesis and progression. METTL3-mediated N6-methyladenosine modification led to LINC00958 upregulation through stabilizing its RNA transcript. A PLGA-based nanoplatform loaded with si-LINC00958 was developed for HCC systemic administration. This novel drug delivery system was controlled release, tumor targeting, safe, and presented satisfactory antitumor efficacy. CONCLUSIONS: Our results delineate the clinical significance of LINC00958 in HCC and the regulatory mechanisms involved in HCC lipogenesis and progression, providing a novel prognostic indicator and promising nanotherapeutic target.

11.
Food Chem ; 312: 126080, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-31896451

RESUMO

The influence of ultrasonic pretreatment on the structure, antioxidant activity, and IgG/IgE binding activity of ß-lactoglobulin (ß-Lg) during digestion in vitro were studied by spectroscopy, chromatography and ELISA. After ß-Lg was treated by ultrasonic prior to digestion in vitro, the treated ß-Lg showed high intrinsic fluorescence emission, more of the hydrolytic products and high antioxidant activity, which depend on the proteolysis promoted by ultrasonic, and produced many small-molecule antioxidant peptides. Native ß-Lg was resistant to gastric digestion and retained its allergenicity. However, the allergenicity of ultrasonicated ß-Lg after gastric digestion was increased due to ultrasonic promotes the production of peptides with intact structure and immunogenicity. Subsequent gastrointestinal digestion, ultrasonicated ß-Lg has a diametrically opposite results because the increase of small peptides with the decreasing of immunogenicity. Therefore, the structural changes of ß-Lg by ultrasonic and gastrointestinal digestion were responsible for improving the antioxidant activity and reducing the IgG/IgE binding activity.

12.
Bioorg Chem ; 95: 103546, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31911302

RESUMO

Twenty new ingol diterpenoids, euphornans A-T (1-20), representing a rare class of C-19-oxidated and H-2, H-3 ß-oriented ingols, were isolated from the seeds of Euphorbia marginata. Their structures including absolute configurations were elucidated by extensive spectroscopic analysis, ECD analysis, and single crystal X-ray diffraction. Compounds 1-20 were screened for the multidrug resistance (MDR) reversal activity on P-glycoprotein (Pgp)-dependent MDR cancer cell line HepG2/ADR, and 11, 14, and 18 were identified as potent MDR modulators that could enhance the efficacy of anticancer drug adriamycin to ca. 20 folds at 5 µM. The Pgp inhibition mechanism and brief structure-activity relationships (SARs) of these compounds were also discussed.

13.
Nat Commun ; 11(1): 554, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31992693

RESUMO

Agriculture (e.g., rice paddies) has been considered one of the main emission sources responsible for the sudden rise of atmospheric methane concentration (XCH4) since 2007, but remains debated. Here we use satellite-based rice paddy and XCH4 data to investigate the spatial-temporal relationships between rice paddy area, rice plant growth, and XCH4 in monsoon Asia, which accounts for ~87% of the global rice area. We find strong spatial consistencies between rice paddy area and XCH4 and seasonal consistencies between rice plant growth and XCH4. Our results also show a decreasing trend in rice paddy area in monsoon Asia since 2007, which suggests that the change in rice paddy area could not be one of the major drivers for the renewed XCH4 growth, thus other sources and sinks should be further investigated. Our findings highlight the importance of satellite-based paddy rice datasets in understanding the spatial-temporal dynamics of XCH4 in monsoon Asia.

14.
Exp Cell Res ; 387(2): 111806, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31891681

RESUMO

GRWD1 is a member of the WD repeat protein family that is over-expressed in various cancer cell lines and associated with poor prognosis in patients with cancer. However, its biological function and mechanism in non-small cell lung cancer (NSCLC) remain unclear. In this study, we aimed to elucidate the role of GRWD1 in NSCLC. Immunohistochemistry on tumor specimens from 170 patients showed that GRWD1 is highly expressed in NSCLC tissues and positively correlated with tumor size, lymph node metastasis, and P-TNM stage, but negatively correlated with differentiation and prognosis. We found that GRWD1 promotes cell colony formation by affecting the expression of Cyclin B1, CDK1, and p27 and inducing G2/M transition. GRWD1 was also found to stimulate cell migration through RhoA, RhoC, and CDC42, and induce epithelial-mesenchymal transition by affecting the expression of E-cadherin, N-cadherin, Vimentin, Snail, Zeb1, and ZO-1. Our results indicated that the GRWD1 can activate the Notch signaling pathway by affecting the Notch intracellular domain and promoting the expression of Hes1. Our use of DAPT to suppress Notch signaling confirmed that GRWD1 promotes the progression of NSCLC through the Notch signaling pathway and may be a potential prognostic biomarker and therapeutic target for this disease.

15.
Talanta ; 210: 120620, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31987167

RESUMO

Boronate affinity is widely used for the isolation of glycoproteins at alkaline conditions. For further proteomic studies, however, it is of highly importance to perform protein adsorption in neutral medium. For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid. The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0). The moiety of sodium alginate (SA) in the hybrid further improves the isolation/enrichment capacity for glycoproteins through hydrophilic interaction. The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 µg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%. The adsorption behavior of IgG fits Langmuir adsorption model, offering a maximum adsorption capacity of 495 mg g-1. The CPBA-Ni6PW9/SA hybrid is practically applied for the enrichment of glycoproteins from human serum. SDS-PAGE assay result indicates that approximately 92% serum albumin is eliminated and high-purity IgG is obtained. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis clearly demonstrated that after enriching with CPBA-Ni6PW9/SA, 81 glycoproteins are identified and 79.4% recognition selectivity is achieved.

16.
Food Funct ; 11(1): 759-767, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31915755

RESUMO

Vitamin A and its precursor (ß-carotene) have been linked with cancer incidence and mortality. However, the relationship between vitamin A and the prognosis of hepatocellular-carcinoma (HCC) is still unknown. Therefore, we investigated whether dietary intakes of vitamin A, retinol, and ß-carotene were associated with survival in patients with HCC who participated in the Guangdong Liver Cancer Cohort (GLCC) study. Patients aged 18-80 years with a diagnosis of incident Primary Liver Cancer (PLC) were enrolled within one month of diagnosis prior to cancer treatment at the Sun Yat-sen University Cancer Center. Dietary information one year before diagnosis of HCC was obtained using a 79-item, validated semiquantitative food frequency questionnaire (FFQ). We restricted the present analysis to 877 HCC patients enrolled in the GLCC between September, 2013 and April, 2017 who had completed FFQ. Cox proportional hazard regression models were used to estimate hazard ratios (HR) and 95% confidence intervals (CIs) for overall and HCC-specific survival. After a median follow-up of 797 days, 384 deaths were documented, 343 of which died from HCC. The multivariable-adjusted HRs (95% CI) of overall and HCC-specific survival for the highest versus the lowest quartile were 0.70 (0.53-0.94) and 0.68 (0.50-0.92) for vitamin A, and 0.72 (0.54-0.96) and 0.69 (0.51-0.94) for ß-carotene, respectively. However, no significant association of dietary retinol intakes with survival outcomes was observed. Our observations suggest that higher prediagnostic dietary intakes of vitamin A and ß-carotene were associated with improved overall and HCC-specific survival.

17.
Bosn J Basic Med Sci ; 2020 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-31999940

RESUMO

The diagnostic performance of the Liver Imaging Reporting and Data System (LI-RADS) in differentiating hepatocellular carcinoma (HCC) from other hepatic malignancies has not been investigated in Chinese patients with chronic liver disease from hepatitis B virus (HBV) infection. The aim of this study was to evaluate the accuracy of the LI-RADS version 2018 in differentiating HCC, intrahepatic cholangiocarcinoma (ICCA), and combined HCC-cholangiocarcinoma (cHCC-CCA) in Chinese patients with HBV infection. Seventy consecutive HBV-infected patients with ICCA (n = 48) or cHCC-CCA (n = 22) who underwent contrast-enhanced magnetic resonance imaging (CE-MRI) between 2006 and 2017 were enrolled along with a comparison cohort of 70 patients with HCC and CE-MRI-matched for tumor size (10-19 mm, 20-30 mm, 31-50 mm, and >50 mm). Imaging feature frequencies for each tumor type were compared using Fisher's exact test. The classification accuracy of LR-5 and LR-M was estimated for HCC versus non-HCC (ICCA and cHCC-CCA). The interobserver agreement was good for LI-RADS categories of HCC, and moderate for non-HCC. After consensus read, 66 of 70 (94%) HCCs were categorized LR-5 (including tumor in vein [TIV] with LR-5), while 42 of 48 (88%) ICCAs and 13 of 22 (59%) cHCC-CCAs were categorized LR-M (including TIV with LR-M) (p < 0.001). Thus, assignment of LR-5 provided 94% sensitivity and 81% specificity for HCC. LR-M provided 79% sensitivity and 97% specificity for non-HCC (ICCA and cHCC-CCA); and the sensitivity and accuracy were lower in differentiating HCC from non-HCC (tumor size <20 mm). LI-RADS v2018 category 5 and M reliably differentiated HBV-related HCC from ICCA. However, a substantial proportion of cHCC-CCAs were categorized LR-5 rather than LR-M. While management is controversial for these combined tumors, accurate prospective differentiation is desired for optimal treatment.

18.
HPB (Oxford) ; 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31980306

RESUMO

BACKGROUND: Aging of the population and prolonged life expectancy have significantly increased the number of elderly patients undergoing hepatectomy for hepatocellular carcinoma (HCC). However, potential benefits, especially long-term oncologic outcomes of hepatectomy for elderly patients with HCC remain unclear. METHOD: Patients treated with curative-intent hepatectomy for HCC in 8 Chinese hospitals were enrolled. Patients were divided into the elderly (≥70 years old) and younger (<70 years old) groups. Overall survival (OS), cancer-specific survival (CSS), and time-to-recurrence (TTR) were compared. Risk factors of CSS and TTR were evaluated by univariable and multivariable competing-risk regression analyses. RESULTS: Of 2134 patients, 259 (12.1%) and 1875 (87.9%) were elderly and younger aged, respectively. Postoperative 30-day and 90-day mortality was comparable among elderly and younger patients. Compared with younger patients, the elderly had a worse 5-year OS (49.4% vs. 55.3%, P = 0.032), yet a better 5-year CCS (74.5% vs. 61.0%, P = 0.005) and a lower 5-year TTR (33.7% vs. 44.9%, P < 0.001), respectively. Multivariable analyses identified that elder age was independently associated with more favorable CSS (HR 0.74, 95%CI 0.58-0.90, P = 0.011) and TTR (0.69, 0.53-0.88, P < 0.001) but was not associated with OS (P = 0.136). CONCLUSIONS: Age by itself is not a contraindication to surgery, and selected elderly patients with HCC can benefit from hepatectomy. Compared with younger patients, elderly patients have noninferior oncologic outcomes following hepatectomy for HCC.

19.
Curr Microbiol ; 77(2): 220-231, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31802201

RESUMO

The mitochondrial citrate transport system, composed of citrate and malate transporters (MTs), can regulate the citrate efflux from mitochondria to cytosol, and then citrate is cleaved into OAA and acetyl-CoA which can be used for fatty acid (FA) biosynthesis. However, in the fungus Mucor circinelloides the molecular mechanism of citrate efflux from the mitochondria by this system and its role in FA synthesis is unclear. In the present study, we have analyzed the genome of high lipid-producing strain WJ11 and the low lipid-producing strain CBS 277.49 to find the potential genes involving in this system. Five potential genes are present in the genome of WJ11. These genes encode one citrate transport protein (CT), one tricarboxylate carrier (TCT), one MT, and two 2-oxoglutarate:malate antiporters (SoDIT-a and SoDIT-b). However, the genome of CBS 277.49 contains the same set of genes, except for the presence of just one SoDIT. The proteins from WJ11 had similar properties as their counterparts in CBS 277.49. Moreover, phylogenetic analyses revealed the evolutionary relationship of these proteins and illuminated their typical motifs related to potential functions. Additionally, the expression of these genes was analyzed to predict the possible functions in lipid metabolism in M. circinelloides. This is the first study to report the in silico analysis of structures and functions of the mitochondrial citrate transport system in M. circinelloides. This work showed a new strategy for research for the selection of candidate genes for further detailed functional investigation of the mitochondrial citrate transport system in lipid accumulation.

20.
Acta Biomater ; 103: 165-171, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31812842

RESUMO

Long-distance waveguiding and submicron focusing of light in a bio-medium are crucial for biomedical sensing and imaging. Disordered bio-mediums usually exhibit high scattering and absorption, which limits effective waveguiding and focusing. Here, we demonstrate an optofluidic cell chain, assembled via an optical trapping force from an optical fiber probe, to achieve long-distance waveguiding and submicron light focusing in a disordered bio-medium. By applying a trapping light at 980 nm to generate an optical force, stable binding of E. faecalis cells was achieved in a fluid to assemble cell chains of different lengths. The length could reach up to 360 µm and the incident light (at 675, 532 and 473 nm) could be focused into a beam with a waist radius of 400 nm. As a potential practical application, backscattered signals from human red blood cells were detected using the cell chains, which is expected to benefit biomedical sensing and single cell analysis. STATEMENT OF SIGNIFICANCE: With the assistance of optofluidic techniques, we assembled an E. faecalis cell chain with a length up to 360 µm to achieve long-distance waveguiding and submicron focusing at a propagation loss of 0.03 dB/µm in the bio-medium. Visible lights were launched into the cell chain and the incident lights can converge into a beam with a waist radius of 400 nm. The cell chain was further used to detect the backscattering signals from human red blood cells (RBCs), and the results indicate that the cell chain can be applied as a fully biocompatible extension of the probe for the real-time detection of RBCs in healthy and pathological states.

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