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1.
J Ethnopharmacol ; 282: 114656, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-34551361

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Aidi injection is one of the China Food and Drug Administration approved Chinese herbal injections and the most competitive product in cancer care in China. It is composed of the extracts from Mylabris Phalerata, Astragalus Membranaceus, Panax Ginseng, and Acanthopanax Senticosus. AIM OF THE STUDY: This overview aims to map systematic reviews (SRs) of Aidi injection for cancer and provide a summarized evidence for clinical practice and decision making. MATERIALS AND METHODS: Seven databases were searched for SRs and/or meta-analyses of randomized controlled trials on Aidi injection for cancer care until December 2020. Six authors worked in pairs independently identified studies, collected data, and assessed the quality of included studies according to the revised Assessment of Multiple Systematic Reviews (AMSTAR 2) and the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). A narrative synthesis was used for the evidence mapping. RESULTS: Fifty-two SRs on Aidi injection as adjuvant therapy were included, involving lung cancer (20 SRs), liver cancer (10), colorectal cancer (7), gastric cancer (6), lymphoma (2), breast cancer (2), esophageal cancer (1), ovary cancer (1), and a mix of different cancers (4). Except for one SR focusing on Aidi injection used alone, other SRs evaluated Aidi injection in combination with chemotherapy (43), radiotherapy (4), or chemo/radiology/targeting therapy (4). Aidi injection showed additional beneficial effects on survival (9), objective response rate (44), quality of life (42), and the reduction of side-effects from chemo/radiotherapy (48). Using AMSTAR 2 tool, two reviews were assessed as low and the rest as critically low methodological quality mainly due to the lack of prospective registration. The reporting quality was insufficient assessed with PRISMA in the reporting of search strategy (26, 50.0%), additional analysis (19, 36.5%), and the summary of evidence (2, 3.8%). CONCLUSION: Aidi injection has been evaluated for its adjuvant beneficial effects on cancer survival, tumor responses, quality of life, and reducing the side effects of chemo/radiotherapy, mainly focusing on lung, liver and colorectal cancer. The methodological and reporting quality are weak and need to be improved in the future.

2.
Theriogenology ; 177: 84-93, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34687940

RESUMO

MicroRNAs (miRNAs) are ∼22 nt RNAs that direct post-transcriptional repression of mRNA targets in diverse eukaryotic lineages. Granulosa cells (GCs) are the earliest differentiated follicular somatic cells. From the initiation of primordial follicles, their differentiation and growth are closely related to the development of follicles. The research on follicular development mostly focused on the granular layer, as well as the hormone synthesis induced by granulosa cell differentiation before and after follicular selection. In this study, we evaluated the effects of miR-23b-3p on chicken granulosa cells, including granulosa cell proliferation and steroid hormone synthesis. Elevated expression of miR-23b-3p significantly inhibited granulosa cell proliferation and steroid hormone synthesis, but did not affect apoptosis. Furthermore, it was observed that the forecast growth differentiation factor 9 (GDF9) is a target gene of miR-23b-3p and miR-23b-3p can down-regulate expression of GDF9. Overall, this study demonstrated that miR-23b-3p can regulate the proliferation and steroid hormone synthesis of chicken granulosa cells by inhibiting the expression of GDF9.


Assuntos
Fator 9 de Diferenciação de Crescimento , MicroRNAs , Animais , Proliferação de Células , Galinhas/genética , Feminino , Fator 9 de Diferenciação de Crescimento/genética , Hormônios , MicroRNAs/genética , Esteroides
3.
Environ Pollut ; 292(Pt A): 118263, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34601035

RESUMO

The willingness of migrating due to air pollution is widespread in China. However, there is a lack of direct evidence and discussion regarding whether this willingness has been translated into action. In this study, PM2.5 concentrations were used to represent air pollution in each city and were compared with individual migration data from the China Labor-force Dynamics Survey (CLDS) to examine population migration effects caused by air pollution. This study showed that (1) Population migration between Chinese cities shows sensitivity to air pollution, and air pollution increases the probability of moving away for local population. This finding is held under multiple robustness and endogeneity tests. (2) Population migration effects caused by air pollution were more pronounced among women, middle-aged people, those with lower educational levels, from agricultural households, Han Chinese groups, and populations in southern cities. (3) The use of individual self-rated health data verified that physical health is an important channel through which individual migration decisions are influenced by air pollution, the older an individual, the more his or her health was affected. In light of these findings, this study led to conclusions regarding targeted policy recommendations in terms of talent clustering, social equity, and demographic balance.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Poluentes Atmosféricos/análise , Poluição do Ar/análise , China , Cidades , Monitoramento Ambiental , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Material Particulado/análise
4.
Sci Total Environ ; 804: 149965, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34508937

RESUMO

Ammonium removal in wastewater treatment plants demands large quantities energy input, such as aeration for wastewater and the addition of organics for nitrate reduction. Anaerobic ammonium oxidation coupled to Fe(III) reduction, called Feammox process play a crucial role in natural nitrogen cycle, which has been rarely investigated in the field of wastewater treatment. Besides, Iron-reducing bacteria (FeRB) as function bacteria of Feammox could transfer electrons to iron oxide by oxidizing organics. The possibility of anaerobic ammonium removal coupled with organics should be investigated to assess the potential of Feammox process for conventional wastewater treatment. In this study, five Fe(III) compounds, Fe2O3, Fe3O4, Fe(OH)3, Citrate-Fe and pyrite were supplemented to investigate the effect of iron oxides on ammonium removal in serum bottles with working volume of 100 mL. It was found that ammonium removal efficiency of the Fe2O3 group was the highest. To simulate wastewater treatment process in sewage treatment plant, three Up-flow anaerobic sludge blanket reactors with volume of 250 mL adding Fe2O3 were applied with influent of ammonium and carbon sources. It was found that the organics significantly inhibited the ammonium removal by Feammox process. This was attributed to that carbon sources and ammonium could be used as electron donors for Fe(III) reduction. In addition, this nitrogen removal was also likely related with the iron cycle, i.e., Fe(III) reduction with ammonium oxidation and Fe(II) oxidation with nitrate/nitrite reduction. This study provides a promising alternative technology for anaerobic ammonium removal in wastewater treatment. Optimizing nitrogen removal and carbon sources applied in conventional wastewater plants are required in future.


Assuntos
Compostos de Amônio , Purificação da Água , Anaerobiose , Reatores Biológicos , Compostos Férricos , Nitrogênio , Ciclo do Nitrogênio , Oxirredução
5.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120282, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34454131

RESUMO

The conversion of p-aminothiophenol (PATP) or p-nitrothiophenol (PNTP) to p,p'-dimercaptoazobenzene (DMAB) has been used as model reactions to study plasmon-catalyzed reaction on nanoparticles. Herein, we report the conversion of PNTP to DMAB which is triggered by SO32- ions on gold nanoparticles (AuNPs) for the first time. With the addition of SO32-, the Raman peaks at 1139, 1392, 1437 cm-1 appears, which indicates the formation of DMAB. The experiment results suggested that the synergistic effect of AuNPs and SO32- promoted the conversion of PNTP to DMAB. Besides, the proposed catalysis system is high selectivity to SO32- ions, which provides a new detection route to SO32- ions in the future. More importantly, the possible reaction mechanism has been put forward which is helpful to understand the surface plasmon-assisted catalytic reduction of PNTP on the surface of SERS substrate.


Assuntos
Ouro , Nanopartículas Metálicas , Catálise , Análise Espectral Raman , Compostos de Sulfidrila , Sulfitos
6.
Environ Res ; 203: 111796, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34339698

RESUMO

Treatment of organic wastewater is a challenging task. Biological techniques using biocatalysts have shown their benefits in organic wastewater treatment. In this research, a novel biocatalyst was developed by encapsulation of Fe3O4 microspheres and haemoglobin (Hb) with mesoporous silica, named Fe3O4@mSiO2(Hb). Fe3O4@mSiO2(Hb) exhibited typical mesoporous characteristics (mesoporous silica), magnetic feature (Fe3O4) and peroxidase activity (Hb). The results showed that the immobilization of Hb into Fe3O4@mSiO2 did not affect its activity. In addition, Fe3O4@mSiO2(Hb) exhibited a higher efficiency in the peroxidation of aromatic compounds than free Hb. The peroxidase activity of the synthesized biocatalyst was estimated to be 120 Ug-1, which was almost four times greater than that of previously reported immobilized Hb. Also, the Km of Fe3O4@mSiO2(Hb) was similar to that of the free Hb and it was estimated to be 4.3 × 10-4 µM, indicating that the activity of the Hb in the immobilized enzyme was not affected after immobilization. The immobilized enzyme was also found to be stable, recyclable and reusable. Taken together, these results indicate that the Fe3O4@mSiO2(Hb) has good potential to be used for treating organic wastewater containing aromatic compounds. The magnetically separable novel biocatalyst developed in this study provided not only a more suitable microenvironment for retaining the activity of Hb, but also demonstrated enhanced stability and activity under unfavorable conditions.

7.
Small ; : e2105308, 2021 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-34741427

RESUMO

The ever-increasing development of flexible and wearable electronics has imposed unprecedented demand on flexible batteries of high energy density and excellent mechanical stability. Rechargeable lithium (Li) metal battery shows great advantages in terms of its high theoretical energy density. However, the use of Li metal anode for flexible batteries faces huge challenges in terms of its undesirable dendrite growth, poor mechanical flexibility, and slow fabrication speed. Here, a highly scalable Li-wicking strategy is reported that allows ultrafast fabrication of mechanically flexible and electrochemically stable Li metal anodes. Through the rational design of the interface and structure of the wicking host, the mean speed of Li-wicking reaches 10 m2 min-1 , which is 1000 to 100 000 fold faster than the reported electrochemical deposition or thermal infusion methods and meets the industrial fabrication speed. Importantly, the Li-wicking process results in a unique 3D Li metal structure, which not only offers remarkable flexibility but also suppresses the dendrite formation. Paring the Li metal anode with lithium-iron phosphate or sulfur cathode yields flexible full cells that possess a high charging rate (8.0 mA cm-2 ), high energy density (300-380 Wh kg-1 ), long cycling stability (over 550 cycles), and excellent mechanical robustness (500 bending cycles).

8.
Front Cell Dev Biol ; 9: 752753, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34746146

RESUMO

Introduction: Type 2 diabetes mellitus (T2DM) is an independent risk factor of Alzheimer's disease (AD), and populations with mild cognitive impairment (MCI) have high incidence to suffer from AD. Therefore, discerning who may be more vulnerable to MCI, among the increasing T2DM populations, is important for early intervention and eventually decreasing the prevalence rate of AD. This study was to explore whether the change of plasma ß-amyloid (Aß) could be a biomarker to distinguish MCI (T2DM-MCI) from non-MCI (T2DM-nMCI) in T2DM patients. Methods: Eight hundred fifty-two T2DM patients collected from five medical centers were assigned randomly to training and validation cohorts. Plasma Aß, platelet glycogen synthase kinase-3ß (GSK-3ß), apolipoprotein E (ApoE) genotypes, and olfactory and cognitive functions were measured by ELISA, dot blot, RT-PCR, Connecticut Chemosensory Clinical Research Center (CCCRC) olfactory test based on the diluted butanol, and Minimum Mental State Examination (MMSE) test, respectively, and multivariate logistic regression analyses were applied. Results: Elevation of plasma Aß1-42/Aß1-40 is an independent risk factor of MCI in T2DM patients. Although using Aß1-42/Aß1-40 alone only reached an AUC of 0.631 for MCI diagnosis, addition of the elevated Aß1-42/Aß1-40 to our previous model (i.e., activated platelet GSK-3ß, ApoE ε4 genotype, olfactory decline, and aging) significantly increased the discriminating efficiency of T2DM-MCI from T2DM-nMCI, with an AUC of 0.846 (95% CI: 0.794-0.897) to 0.869 (95% CI: 0.822-0.916) in the training cohort and an AUC of 0.848 (95% CI: 0.815-0.882) to 0.867 (95% CI: 0.835-0.899) in the validation cohort, respectively. Conclusion: A combination of the elevated plasma Aß1-42/Aß1-40 with activated platelet GSK-3ß, ApoE ε4 genotype, olfactory decline, and aging could efficiently diagnose MCI in T2DM patients. Further longitudinal studies may consummate the model for early prediction of AD.

9.
iScience ; 24(11): 103223, 2021 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-34746696

RESUMO

Serine/threonine kinase 39 (STK39) is overexpressed in various tumor tissues and plays an essential role in tumor progression. In this study, we investigated the clinical value, as well as the potential functions and mechanisms of STK39 in cholangiocarcinoma (CCA). The results showed that STK39 was overexpressed in CCA and negatively associated with the prognosis of patients with CCA. Functionally, STK39 knockdown suppressed cell proliferation, migration, and invasion, while STK39 overexpression facilitated tumor aggressiveness. The tumor-promoting effects of STK39 in CCA were also validated by in vivo experiments. Mechanistically, RNA-seq analysis identified that STK39 enhanced the progression of CCA by activating PI3K/AKT signaling pathway. Furthermore, overexpression of STK39 could induce gemcitabine resistance in CCA cells. Moreover, the increased expression of STK39 may be mediated by the dysregulation of miR-26a-5p. In summary, STK39 could be served as a valuable prognostic candidate and a potential therapeutic target of CCA.

10.
Educ Inf Technol (Dordr) ; : 1-22, 2021 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-34754268

RESUMO

Learning motivation is crucial to online learning success, especially for K-12 students. Although previous research has proved that there are many factors influencing online learning motivation, few studies have systematically investigated this phenomenon from the integrated perspectives of community of inquiry and technology acceptance, two theoretical frameworks that are commonly used to explain experiences of and attitude towards online learning. This study investigates the effects of K-12 students' perceived presence and technology acceptance on their online learning motivation. A total of 13,610 valid questionnaires were collected from K-12 students from Wuhan and adjacent areas in central China participated in the survey. The findings reveal that: (1) perceived usefulness, self-efficacy, social presence, and perceived ease of use have a larger positive effect on online learning motivation, while cognitive presence has a small positive effect on online learning motivation; (2) teaching presence positively influences online learning motivation through social or cognitive presence; (3) factors, such as school location, previous online learning experience, family social-economic status, and prior academic achievements, may influence technology acceptance. The implications for designing, developing, and managing K-12 online education are discussed.

11.
Front Endocrinol (Lausanne) ; 12: 722861, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34759889

RESUMO

Objective: We aimed to investigate the alterations of brain functional connectivity (FC) in type 2 diabetes mellitus (T2DM) patients without clinical evidence of cognitive impairment and microvascular complications (woCIMC-T2DM) using resting-state functional MRI (rs-fMRI) and to determine whether its value was correlated with clinical indicators. Methods: A total of 27 T2DM and 26 healthy controls (HCs) were prospectively examined. Cognitive impairment was excluded using the Mini-Mental State Examination (MMSE) and the Montreal Cognitive Assessment (MoCA) scales, and microvascular complications were excluded by fundus photography, microalbuminuria, and other indicators. The correlation maps, derived from rs-fMRI with posterior cingulate cortex (PCC) as the seed, were compared between T2DM patients and HCs. Pearson's correlation analysis was performed to determine the relationship between the FC of PCC and the clinical indicators. Results: Compared with HC, woCIMC-T2DM patients showed significantly decreased FCs with PCC (PCC-FCs) in the anterior cingulate cortex (ACC), right superior frontal gyrus, right medial frontal gyrus, and right angular gyrus. Meanwhile, increased PCC-FCs was observed in the right superior temporal gyrus and calcarine fissure (CAL). The FC of PCC-ACC was negatively correlated with glycosylated hemoglobin (HbA1c) and diabetes duration, and the FC of PCC-CAL was significantly positively correlated with HbA1c and diabetes duration. Conclusion: The FC, especially of the PCC with cognitive and visual brain regions, was altered before clinically measurable cognitive impairment and microvascular complications occurred in T2DM patients. In addition, the FC of the PCC with cognitive and visual brain regions was correlated with HbA1c and diabetes duration. This indicates that clinicians should pay attention not only to blood glucose control but also to brain function changes before the occurrence of adverse complications, which is of great significance for the prevention of cognitive dysfunction and visual impairment.

12.
Medicine (Baltimore) ; 100(46): e27669, 2021 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-34797290

RESUMO

ABSTRACT: To provide reliable molecular markers and effective therapeutic targets for chondrosarcoma and glioma.Gene Set Enrichment (GSE) 29745 and GSE48420 were downloaded from the Gene Expression Omnibus (GEO) database. Differently expressed genes (DEGs) were identified by the GEO2R. We annotated the function of common DEGs through Digital Audio/Video Interactive Decoder (DAVID) and Metascape. Protein-protein interaction network construction was performed through STRING. Hub genes were identified by the two different algorithms (MCC, EPC). DDX10 and BYSL were key factors in embryo implantation and development, and plays a role in a variety of cancers. The role of the DDX10 and BYSL on the glioma derived from the chondrosarcoma would be explored by the clinical samples.A total of 1442 DEGs were identified. The variations in DEGs were mainly enriched in vasculature development, cell motion, blood vessel development, cell migration, regulation of cell proliferation, regulation of cell proliferation, wound healing, biological adhesion, growth factor binding, identical pathways in cancer, and p53 signaling pathway. Dead-box helicase 10 (DDX10), Bystin-like (BYSL), and WD repeat domain 12 (WDR12) were identified as the hub genes, and the three hub genes were up-regulated in the chondrosarcoma. Chondrosarcoma patients with high expression levels of DDX10 (Logrank P = .0052; HR (high) = 1.8; n (high) = 131, 50%), and BYSL (P = 6.5e-05; HR (high) = 2.3; n (high) = 131, 50%) had poorer overall survival times than those with low expression levels.DDX10 and BYSL genes may provide reliable molecular markers and effective therapeutic targets for chondrosarcoma and glioma.

13.
Chemosphere ; : 132948, 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34800504

RESUMO

Antibiotic and heavy metal residues in shallow lakes caused by aquaculture and human activities such as sewage discharge have attracted much attention and public concern. However, mechanisms by which these environmental pollutants affect the microorganism-mediated biogeochemical cycle are unknown. This study focused on the effects of antibiotics, heavy metal, and antibiotic resistance genes (ARGs) on denitrification in shallow lakes. The results showed that antibiotics and metal elements had inhibitory effects on denitrification, whereas AGRs exhibited stimulating effects. Specifically, the enrofloxacin concentration showed a significant negative correlation with the copy number of denitrifying bacteria, whereas the copy number of the ARGs sulI, sulII, and tetG showed significant positive correlations. In addition, tetG was closely related to the community structure of nirS-type denitrifiers, and nirS-type denitrifiers were significantly correlated with the potential denitrification rate (PDR). Furthermore, the ARGs sulI, sulII, and tetG were positively correlated with PDR (P < 0.05). By contrast, the metal elements arsenic, manganese, cobalt, and antimony were negatively correlated with the copy number of denitrifying bacteria. Arsenic was significantly correlated with the community composition of nirK-type denitrifiers, but nirK-type denitrifiers did not show a significant correlation with the PDR. This work extends our understanding of the effects of antibiotics and heavy metals on denitrification, but further studies are needed to determine the interaction effects of pollutants.

14.
Adv Med Sci ; 67(1): 29-38, 2021 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-34784538

RESUMO

PURPOSE: Oxidative damage and intestinal flora dysbiosis play important roles in the progression of chronic ulcerative colitis (UC). This study explored the effect and mechanism of molecular hydrogen in chronic UC. MATERIALS AND METHODS: Male C57BL/6 mice (19.6 â€‹± â€‹0.4 â€‹g, 7 weeks) were randomly divided into 3 groups: normal control (NC) group, UC (Dextran Sulfate Sodium, DSS) group, and hydrogen-rich water (HRW, 0.8 â€‹ppm)-treated UC (DSS â€‹+ â€‹HRW) group. Mice in the DSS treatment group were treated with DSS for the following 3 cycles to establish chronic UC model: the first 2 cycles consisted of 2.5% DSS for 5 days, followed by drinking water for 16 days, and a third cycle consisted of 2% DSS for 4 days, followed by drinking water for 10 days. The mice in the DSS â€‹+ â€‹HRW group were administered HRW daily throughout the experiment. RESULTS: The mice in the DSS groups developed typical clinical signs of colitis. HRW treatment partially ameliorated colitis symptoms, improved histopathological changes, significantly increased glutathione (GSH) concentration and decreased TNF-α level. Notably, HRW treatment significantly inhibited the growth of Enterococcus faecalis, Clostridium perfringens and Bacteroides fragilis (P â€‹< â€‹0.05 vs. DSS group), with the relative abundance that was close to the levels in the NC group. Microarray analysis revealed that 252 genes were significantly modified after HRW treatment compared with those in the DSS treatment alone group, and 17 genes were related to inflammation, including 9 interferon-stimulated genes (ISGs). CONCLUSIONS: Hydrogen-rich water partially alleviates inflammation, oxidative stress and intestinal flora dysbiosis in DSS-induced chronic UC mice.

15.
Int J Gen Med ; 14: 7961-7969, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34785940

RESUMO

Background: Benign prostatic hyperplasia (BPH) is a frequent-occurring disease in middle-aged and elderly men. This work is a prospective study and aims at exploring the incidence of post-operative depression and the potential risk factors of depression in a cohort of patients with BPH in China. Methods: In this survey, 611 men who underwent transurethral resection of the prostate (TURP) were strictly selected at our institution from January 2016 to August 2019. Zung Self-rating Depression Scale was used for evaluation of depressive symptoms. Sociodemographic, clinical and other data were also collected. Results: We found that 152/611 (24.9%) patients suffered from different degree of depression at 6 months after TURP, including mild symptoms (20.9%) and moderate/severe symptoms (3.9%). A total of 421 (68.9%) patients developed post-TURP erectile dysfunction (ED). The occurrence of depression was closely associated with marital status, education level, cigarette smoking, alcohol consumption, severity of lower urinary tract symptoms (LUTS), duration of BPH, erectile function, and comorbidities (such as diabetes, dyslipidaemia and bladder stone). The risk factors related to the severity of depression included widowed or single marital status, frequent alcohol consumption, moderate or severe LUTS, longer duration (> 5 years) of BPH, ED, urinary continence, and comorbidities such as diabetes and bladder stone. Conclusion: Many risk factors are related to the occurrence of depression in patients undergoing TURP. Widowed or single marital status, frequent alcohol consumption, moderate or severe LUTS, longer duration of BPH, ED, urinary incontinence and comorbidities such as diabetes and bladder stone are connected with the increase odds of moderate or severe depressive symptom.

16.
Food Funct ; 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34788781

RESUMO

Phloretin, a dihydrochalcone, widely exists in the fruits of apple trees and crabapple trees (Malus prunifolia) with multiple biological activities. Presently, we studied the function of phloretin on the attenuation of hepatic steatosis and further explored the underlying mechanisms both in vitro and in vivo. Male C57BL/6J mice were fed a normal diet or high fat diet (HFD) with or without phloretin (100 mg kg-1) for 12 weeks. HepG2 cells were induced by 200 µM palmitic acid (PA) and co-incubated with phloretin (50 µM) for 24 h. The results showed that phloretin treatment significantly decreased the accumulation of lipids in the liver of the HFD-fed C57BL/6J mice and PA-induced HepG2 cells. Also, phloretin effectively ameliorated hepatic steatosis via promoting fatty acid ß-oxidation (FAO). This biological activity of phloretin was closely related to its capacity to improve mitochondrial dysfunction, including the promotion of mitochondrial biosynthesis and inhibition of mitochondrial swelling through the AMPK-dependent SIRT1/PGC-1α and SIRT3/CypD signaling pathways, respectively. These results demonstrate that phloretin effectively improves mitochondrial function and ameliorates HFD-induced hepatic steatosis through an AMPK-dependent signaling pathway.

17.
Adv Mater ; : e2106314, 2021 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-34847272

RESUMO

The implant-related microbial infection is a challenging clinical problem and its treatment requires efficient eradiation of the biofilm from the implant surface. NIR-responsive strategies have been proposed as an emerging efficient antibacterial therapy. However, the utilization of photosensitizer or photocatalytic/photothermal nanomaterials in the available approach likely induces high potential risks of interfacial deterioration and biosafety compromise. Herein, TiO2 /TiO2-X metasurface with potent NIR-responsive antibacterial activity was produced on Ti alloy implant by a newly invented topochemical conversion-based alkaline-acid bidirectional hydrothermal method (aaBH). Electromagnetic simulations proved that NIR absorption and near-field distribution of the metasurface can be tuned by the dimension and arrangement of the nanostructural unit. Promising antibacterial efficacy was proved by both in vitro and in vivo tests, with low power NIR irradiation for 10 mins. Besides, the designed nanostructure in the metasurface itself also showed excellence in enhancing the adhesion-related gene expression of human gingival fibroblasts that were exposed to 10 mins NIR irradiation, proving the potent nanostructure-induced biological effects. This work provides a biosafe and upscalable metasurfacing approach with extraordinary capacity of manipulating light adsorption, photocatalysis, and biological properties. This article is protected by copyright. All rights reserved.

18.
Poult Sci ; 101(1): 101524, 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34784514

RESUMO

Follicular atresia is an important cause of reproductive decline in egg-laying hens. Therefore, a better understanding of the regulation mechanism of follicle atresia in poultry is an important measure to maintain persistent high egg performance. However, how the role of the regulatory relationship between autophagy and apoptosis in the intrafollicular environment affects the follicular atresia of chickens is remain unclear. The objective of this study was to explore the regulatory molecular mechanisms in regard to follicular atresia. 20 white leghorn layers (32-wk-old) were equally divided into 2 groups. The control group was fed freely, and the experimental group induced follicular atretic by fasting for 5 d. The results showed that the expression of prolactin (PRL) levels was significantly higher in the fasted hens, while the levels of luteinizing hormone (LH) and follicle stimulating hormone (FSH) were lower. Most importantly, RNA sequencing, qPCR, and Western blotting detected significantly elevated levels of autophagy and apoptosis markers in atresia follicles. Interestingly, we found that fibromodulin (FMOD) levels was significantly lower in follicles from fasted hens and that this molecule had an important regulatory role in autophagy. FMOD silencing significantly promoted autophagy and apoptosis in granulosa cells, resulting in hormonal imbalance. FMOD was found to regulate autophagy via the transforming growth factor beta (TGF-ß) signaling pathway. Our results suggest that the increase in autophagy and the imbalance in internal homeostasis cause granulosa cell apoptosis, leading to follicular atresia in the chicken ovary. This finding could provide further insight into broodiness in chicken and provide avenues for further improvements in poultry production.

19.
Poult Sci ; 101(1): 101531, 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34823187

RESUMO

Circadian timing system controlled the rhythmic events, for example, ovulation and oviposition in chickens. However, how biological clock mediates eggshell formation remains obscure. Here, A 24-h mRNA transcriptome analysis was carried out in the uterus of 18 chickens with similar oviposition time points to identify the rhythmic genes and to reveal critical genes and biological pathways involved in the eggshell biomineralization. JTK_CYCLE analysis and real-time PCR revealed a total of 1,793 genes from the sequencing database with 23,513 genes (FPKM>1) were rhythmic genes regulating the rhythmic system and the expression of typical clock genes Per2, Cry1, Bmal1, Clock, Per3, and Rev-erbß were rhythmically expressed, which suggested that endogenous clock in uterus might control the eggshell mineralization. Time of peak expression of the rhythmic genes was analyzed based on their acrophase. The main phases clustered at the periods from Zeitgeber time 0 (ZT0) to ZT4 (6:00-10:00) and from ZT10 to ZT14 (16:00-20:00). The rhythmic genes were annotated to the following Gene Ontology terms rhythmic process, lyase, ATP binding, cell membrane component. KEGG pathway enrichment analysis revealed the top 15 rhythmic genes were involved in vital biological pathways, including syndecan (1, 2, 3)-mediated signaling, post-translational regulation of adheres junction stability and disassembly, FoxO family signaling, TGF-ß receptor and transport of small molecular pathways. 166 of total 1,235 genes (13.4%) were defined as rhythmic transfer factors (TFs) and they were investigated expression time distribution of cis-elements of circadian clock system D-box, E-box, B-site, and Y-Box within 24 h. Results indicated that rhythmic TFs at each phase are potential drivers of their circadian transcription activities. Compared with the control, the expression abundances of ion transport elements SCNN1G, CA2, SPP1, and ATP1B1 were significantly decreased after the interference of Bmal1 gene in synchronized uterine tubular gland cells. Clock genes changed their expression along with the eggshell formation, indicating that there is circadian clock in the uterus of chicken and it regulates the expression of eggshell formation genes.

20.
Sensors (Basel) ; 21(22)2021 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-34833560

RESUMO

In view of the demand of location awareness in a special complex environment, for an unmanned aerial vehicle (UAV) airborne multi base-station semi-passive positioning system, the hybrid positioning solutions and optimized site layout in the positioning system can effectively improve the positioning accuracy for a specific region. In this paper, the geometric dilution of precision (GDOP) formula of a time difference of arrival (TDOA) and angles of arrival (AOA) hybrid location algorithm is deduced. Mayfly optimization algorithm (MOA) which is a new swarm intelligence optimization algorithm is introduced, and a method to find the optimal station of the UAV airborne multiple base station's semi-passive positioning system using MOA is proposed. The simulation and analysis of the optimization of the different number of base stations, compared with other station layout methods, such as particle swarm optimization (PSO), genetic algorithm (GA), and artificial bee colony (ABC) algorithm. MOA is less likely to fall into local optimum, and the error of regional target positioning is reduced. By simulating the deployment of four base stations and five base stations in various situations, MOA can achieve a better deployment effect. The dynamic station configuration capability of the multi-station semi-passive positioning system has been improved with the UAV.


Assuntos
Ephemeroptera , Algoritmos , Animais , Simulação por Computador
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