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1.
Food Chem ; 303: 125404, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31466033

RESUMO

Fourier transform infrared (FTIR) spectroscopy calibrations were developed to simultaneously determine the multianalytes of five artificial sweeteners, including sodium cyclamate, sucralose, sodium saccharin, acesulfame-K and aspartame. By combining the pretreatment of the spectrum and principal component analysis, 131 feature wavenumbers were extracted from the full spectral range for modelling to qualitative and quantitative analysis. Compared to random forest, k nearest neighbour and linear discriminant analysis, support vector machine model had better predictivity, indicating the most effective identification performance. Furthermore, multivariate calibration models based on partial least squares regression were constructed for quantifying any combinations of the five artificial sweeteners, and validated by prediction data sets. As shown by the good agreement between the proposed method and the reference HPLC for the determination of the sweeteners in beverage samples, a promising and rapid tool based on FTIR spectroscopy, coupled with chemometrics, has been performed to identify and objectively quantify artificial sweeteners.

2.
J Nanosci Nanotechnol ; 20(2): 701-708, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383065

RESUMO

In the present study we developed novel luminescent magnetic nanocomposites termed Fe3O4@polyaniline/carbon dots. First, Fe3O4 magnetic nanoparticles were prepared by the coprecipitation method. The nanoparticles were then coated with polyaniline using the in situ growth method to form Fe3O4@polyaniline nanohybrids, which were endowed with amino functional groups on the surface and avoided the aggregation of Fe3O4 nanoparticles. The X-ray diffraction pattern demonstrated that the crystalline phase of the Fe3O4 nanoparticles was an inverse spinel structure and was not changed in the Fe3O4@polyaniline nanohybrids. The saturation magnetization and the coercive force of the as-prepared Fe3O4@polyaniline nanohybrids measured by a vibrating sample magnetizer were 63.7 emu·g-1 and zero respectively, which indicated that the Fe3O4@polyaniline nanohybrids exhibited excellent superparamagnetism. The Fe3O4@polyaniline nanohybrids were conjugated with carbon dots, prepared from orange juice, via the amide bond between the amino groups on the surface of the Fe3O4@polyaniline nanohybrids and the carboxyl groups on the surface of carbon dots. The obtained luminescent magnetic nanocomposites Fe3O4@polyaniline/carbon dots showed good photoluminescent properties, which hinted that the nanocomposites have potential in drug tracing and magnetic targeted drug delivery. Finally, the anticancer drug methotrexate was loaded into the Fe3O4@polyaniline/carbon dots nanocomposites, forming a novel magnetic targeted drug delivery system. The results confirmed that the novel drug delivery system exhibited excellent drug-loading capability for methotrexate of ca. 70%, and emits strong fluorescence at the wavelength of 360 nm. An in vitro release experiment of the drug delivery system indicated that the cumulative release percentage of methotrexate was 17.2% in the phosphate-buffered saline (pH = 7.4) within 36 h.

3.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 41(4): 517-523, 2019 Aug 30.
Artigo em Chinês | MEDLINE | ID: mdl-31484615

RESUMO

To analyze the potential associations of ultrasound-guided fine-needle aspiration(FNA),BRAF V600E gene mutation detection,and the combination of these two techniques with the clinicopathological features of papillary thyroid cancer(PTC). Methods Patients with PTC confirmed by surgery from April 2016 to July 2017 were included in this study.The relationship between clinicopathological features and BRAF V600E mutation,FNA results,and the combination of them were explored. Results The sensitivity of FNA was 86.3%(227/263)and the mutation rate of BRAF V600E was 85.9%(226/263)in 263 patients with PTC.The mutation rate of papillary thyroid microcarcinoma(PTMC)was 91.1%(153/168)and that of non-PTMC was 76.8%(73/95).A total of 225 patients underwent lymph node dissection.The lymph node metastasis rate was 35.6%(80/225),and it was 23.8%(34/143)in PTMC,56.1%(46/82)in non-PTMC;in addition,9.9%(26/263)of PTC patients had extracapsular invasion.BRAF V600E mutation rate was higher in patients with the following features:aged over 45 years(P=0.043);the tumor was FNA diagnosed as malignant or suspected malignant(P=0.011);the tumor had a maximum diameter of ≤1 cm(P=0.001);and the primary tumor was in stage T1(P=0.039);however,there was no significant difference in BRAF V600E mutation rate among patients with different sex,capsule invasion,or lymph node metastasis.The diagnostic sensitivity of FNA was not statistically different under different clinical and pathological characteristics.The clinicopathologic features of FNA and BRAF V600E double-positive patients were not significantly different from those of other patients. Conclusion FNA-confirmed malignancy,BRAF V600E gene mutation,and their double-positive results are not correlated with the invasive pathological features of PTC,and thus their roles in guiding an extended operation(or not)are limited.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31485941

RESUMO

Residential coal combustion is one of the main sources of ambient polycyclic aromatic hydrocarbons (PAHs). Updating its emission estimation is limited by the shortages of emission factors, especially for them in different particle sizes and from different combustion conditions. PAH emission factors (EFs) for nine size-segregated particle segments emitted from smoldering and flaming combustion of residential coals (four kinds of raw coals (RCs) and three kinds of honeycomb coal briquettes (HCBs)) were obtained in China, using a dilution sampling system. EFs of PAHs for the flaming and smoldering of HCB ranged from 1.32 to 2.04 mg kg-1 and 0.35 to 5.36 mg kg-1, respectively. The EFs of PAHs for RC flaming combustion varied from 0.50 to 218.96 mg kg-1. About 53.5-96.4% and 47.4-90.9% of PAHs concentrated in PM2.1 and PM1.1, respectively. Different fuel types and combustion conditions strongly affected the PAH EFs. The PAH EF for the RC was 0.3 times that for HCB in Guizhou, which implied that PAH EFs for RC combustion were not always higher than those from HCB burning. For different combustion conditions, the PAH EFs from flaming were more than 2.5 times higher than those from smoldering for HCB except in the Anhui region. Results indicated that current PAH EFs may not be universal, which may bias the establishment of control policies for toxic pollutants emitted from domestic coal burning. On average, 73.2 ± 15.5% of total PAH potential toxicity risks were concentrated in submicron particles. More size-segregated PAH EFs for residential coal combustion should be investigated considering combustion conditions with a uniform sampling method in China.

5.
Viruses ; 11(9)2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31470645

RESUMO

Middle East respiratory syndrome coronavirus (MERS-CoV), a new coronavirus that has been causing severe and fatal acute respiratory illnesses in humans since its outbreak in 2012, has raised public fear worldwide. The development of prophylactics and therapeutics is urgently needed to prevent and control MERS-CoV infections. In this study, a bacterium (Lactococcus lactis)-like particle (BLP) vaccine displaying the MERS-CoV receptor-binding domain (RBD) was developed, and gram-positive enhancer matrix (GEM) particles were used as substrates to externally bind to the MERS-CoV RBD through a protein anchor (PA). The designs included different numbers of lysin motif (LysM) repeats in the PAs linked by linkers (RBD-linker-PA2 (RLP2), RBD-linker-PA3 (RLP3) and RBD-PA3 (RP3)), and three LysM repeats and a linker in the fusion proteins increased the binding activity to the RBD. The specific immune responses were tested by intranasally immunizing mice with RLP3-GEM with or without the adjuvant GEL01. The results showed that GEL01-adjuvanted RLP3-GEM increased the systemic humoral, cellular and local mucosal immune responses in the mouse model, especially in the intestinal tract. The above results indicate that the MERS-CoV BLP product has the potential to be developed into a promising mucosal candidate vaccine to protect against MERS-CoV infections.

6.
Chin J Integr Med ; 2019 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-31471834

RESUMO

OBJECTIVE: To evaluate the association between Chinese medicine (CM) therapy and disease-free survival (DFS) outcomes in postoperative patients with non-small cell lung cancer (NSCLC). METHODS: This multiple-center prospective cohort study was conducted in 13 medical centers in China. Patients with stage I, II, or IIIA NSCLC who had undergone radical resection and received conventional postoperative treatment according to the National Comprehensive Cancer Network (NCCN) guidelines were recruited. The recruited patients were divided into a CM treatment group and a control group according to their wishes. Patients in the CM treatment group received continuous CM therapy for more than 6 months or until disease progression. Patients in the control group received CM therapy for less than 1 month. Follow-up was conducted over 3 years. The primary outcome was DFS, with recurrence/metastasis rates as a secondary outcome. RESULTS: Between May 2013 and August 2016, 503 patients were enrolled into the cohort; 266 were classified in the CM treatment group and 237 in the control group. Adjusting for covariates, high exposure to CM was associated with better DFS [hazard ratio (HR) = 0.417, 95% confidential interval (CI): 0.307-0.567)]. A longer duration of CM therapy (6-12 months, 12-18 months, >24 months) was associated with lower recurrence and metastasis rates (HR = 0.225, 0.119 and 0.083, respectively). In a subgroup exploratory analysis, CM therapy was also a protective factor of cancer recurrence and metastasis in both stage I-IIIA (HR=0.50, 95% CI: 0.37-0.67) and stage IIIA NSCLC postoperative patients (HR = 0.48, 95% CI: 0.33-0.71), DFS was even longer among CM treatment group patients. CONCLUSIONS: Longer duration of CM therapy could be considered a protective factor of cancer recurrence and metastasis. CM treatment is associated with improving survival outcomes of postoperative NSCLC patients in China. (Registration No. ChiCTR-OOC-14005398).

7.
Glycoconj J ; 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31478096

RESUMO

Kashin-Beck disease (KBD) is an endemic degenerative osteoarthropathy of uncertain etiology. Our study sought to identify a correlation between small proteoglycans decorin and biglycan expression and Kashin-Beck Disease. Immunohistochemistry was used to assess the decorin and biglycan levels in cartilage specimens from both child KBD patients, and rats fed with T-2 toxin under a selenium-deficient condition. Real-time PCR and Western blot were used to assess mRNA and protein levels of decorin and biglycan in rat cartilages, as well as in C28/I2 chondrocytes stimulated by T-2 toxin and selenium in vitro. The result showed that decorin was reduced in all zones of KBD articular cartilage, while the expression of biglycan was prominently increased in KBD cartilage samples. Increased expression of biglycan and reduced expression of decorin were observed at mRNA and protein levels in the cartilage of rats fed with T-2 toxin and selenium- deficiency plus T-2 toxin diet, when compared with the normal diet group. Moreover, In vitro stimulation of C28/I2 cells with T-2 toxin resulted in an upregulation of biglycan and downregulation of decorin, T-2 toxin induction of biglycan and decorin levels were partly rescued by selenium supplement. This study highlights the focal nature of the degenerative changes that occur in KBD cartilage and may suggest that the altered expression pattern of decorin and biglycan have an important role in the onset and pathogenesis of KBD.

8.
Expert Opin Drug Metab Toxicol ; : 1-8, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31478386

RESUMO

Introduction: The phosphatidylinositide 3-kinase/AKT/mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway has emerged as an important target in cancer therapy. Numerous PI3K/AKT/mTOR pathway inhibitors are extensively studied; some are used clinically, but most of these drugs are undergoing clinical trials. Potential adverse effects, such as severe hepatotoxicity and pneumonitis, have largely restricted the application and clinical significance of these inhibitors. A summary of mechanisms underlying the adverse effects is not only significant for the development of novel PI3K/AKT/mTOR inhibitors but also beneficial for the optimal use of existing drugs. Areas covered: We report a profile of the adverse effects, which we consider the class effects of PI3K/AKT/mTOR inhibitors. This review also discusses potential molecular toxicological mechanisms of these agents, which might drive future drug discovery. Expert opinion: Severe toxicities associated with PI3K/AKT/mTOR inhibitors hinder their approval and limit long-term clinical application of these drugs. A better understanding regarding PI3K/AKT/mTOR inhibitor-induced toxicities is needed. However, the mechanisms underlying these toxicities remain unclear. Future research should focus on developing strategies to reduce toxicities of approved inhibitors as well as accelerating new drug development. This review will be useful to clinical, pharmaceutical, and toxicological researchers.

9.
Molecules ; 24(17)2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31480226

RESUMO

Four new phenolic components, eurylophenolosides A (1) and B (2), eurylolignanosides A (3) and B (4), along with twelve known compounds were isolated from the roots of Eurycoma longifolia Jack. The structure of these components was elucidated by using various spectral techniques and chemical reactions. Among the known isolates, syringaldehyde (12), 3-chloro-4-hydroxybenzoic acid (13), 3-chloro-4-hydroxyl benzoic acid-4-O-ß-d-glucopyranoside (14), and isotachioside (15) were isolated from the Eurycoma genus for the first time. Further, the NMR data of 14 was reported here firstly. Meanwhile, the nitric oxide (NO) inhibitory activities of all compounds were examined in lipopolysaccharide (LPS)-stimulated RAW264.7 cells at 40 µM. As results, piscidinol A (6), 24-epi-piscidinol A (7), bourjotinolone A (10), and scopoletin (16) were found to play important role in suppressing NO levels without cytotoxicity. Furthermore, the Western blot method was used to investigate the mechanism of compounds 6, 7, 10, and 16 by analysing the level of inflammation related proteins, such as inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in LPS-stimulated RAW264.7 cells. Consequently, compounds 6, 7, 10, and 16 were found to significantly inhibit LPS-induced protein expression of IL-6, NF-κB and iNOS in NF-κB signaling pathway. Moreover, it was found that the protein expression inhibitory effects of 6, 7, and 16 exhibited in a dose-dependent manner. The mechanism may be related to the inhibition of the iNOS expressions through suppressing the IL-6-induced NF-κB pathway.

10.
J Neurooncol ; 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31482267

RESUMO

BACKGROUND AND PURPOSE: microRNAs are small noncoding RNAs that play important roles in cancer regulation. In this study, we investigated the expression, functional effects and mechanisms of action of microRNA-29a (miR-29a) in glioblastoma (GBM). METHODS: miR-29a expression levels in GBM cells, stem cells (GSCs) and human tumors as well as normal astrocytes and normal brain were measured by quantitative PCR. miR-29a targets were uncovered by target prediction algorithms, and verified by immunoblotting and 3' UTR reporter assays. The effects of miR-29a on cell proliferation, death, migration and invasion were assessed with cell counting, Annexin V-PE/7AAD flow cytometry, scratch assay and transwell assay, respectively. Orthotopic xenografts were used to determine the effects of miR-29a on tumor growth. RESULTS: Mir-29a was downregulated in human GBM specimens, GSCs and GBM cell lines. Exogenous expression of miR-29a inhibited GSC and GBM cell growth and induced apoptosis. miR-29a also inhibited GBM cell migration and invasion. PDGFC and PDGFA were uncovered and validated as direct targets of miR-29a in GBM. miR-29a downregulated PDGFC and PDGFA expressions at the transcriptional and translational levels. PDGFC and PDGFA expressions in GBM tumors, GSCs, and GBM established cell lines were higher than in normal brain and human astrocytes. Mir-29a expression inhibited orthotopic GBM xenograft growth. CONCLUSIONS: miR-29a is a tumor suppressor miRNA in GBM, where it inhibits cancer stem cells and tumor growth by regulating the PDGF pathway.

11.
Sao Paulo Med J ; 137(3): 255-261, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31483011

RESUMO

BACKGROUND: There is evidence that genetic predisposition and epigenetic alteration (e.g. DNA methylation) play major roles in lung cancer. In our genetic epidemiological studies, rs1970764 in oncogene PPP1R13L was most consistently associated with lung cancer risk. Here, we explored the role of PPP1R13L methylation in lung cancer development. DESIGN AND SETTING: Analytical cross-sectional study (45 lung cancer cases and 45 controls), conducted in China. METHODS: We investigated the DNA methylation status of 2,160 cytosine-phosphate-guanine (CpG) sites in the PPP1R13L promoter region using the EpiTYPER assay of the Sequenom MassARRAY platform. RESULTS: In the whole study group, the methylation levels of CpG-6, CpG-9, CpG-20 and CpG-21 were significantly lower and those of CpG-16 were significantly higher in cases than in controls. Among smokers, the methylation levels at five CpG sites (CpG-6, CpG-11, CpG-15, CpG-20 and CpG-21) were statistically significantly lower among cases. Among men, the methylation levels at four CpG sites (CpG-11, CpG-15, CpG-20 and CpG-21) were significantly lower among cases. Regarding smokers, the methylation levels at CpG-7.8 and CpG-21 among cases and at CpG-22 among controls were significantly lower, compared with nonsmokers. The frequency of positivity for methylation was not significantly different between lung cancer cases and controls (68.22% for cases and 71.87% for controls; P = 0.119). CONCLUSION: Our study on a Chinese population suggests that lung cancer patients have aberrant methylation status (hypomethylation tended to be more frequent) in peripheral blood leukocytes at several CpG sites in the PPP1R13L promoter region and that exposure to smoking may influence methylation status.

12.
J Acoust Soc Am ; 146(2): EL111, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31472552

RESUMO

This letter presents a model for mapping sea surface observations to the spectra of underwater ambient noise through the self-organizing map method (SOM). The data used to train and test the proposed model include observations of wind speed, atmospheric pressure, and significant wave height from public databases, as well as observations of ambient noise from two deep-water experiments. SOM extracts nonlinear relations from the data and is more suitable for the study of nonlinear dynamics in the ocean than conventional methods. Results indicate the proposed model is reliable with coefficients of determination above 0.9 and root-mean-square errors below 1 dB.

13.
Cancer Med ; 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31475468

RESUMO

Pancreatic cancer (PC) remains one of the deadliest malignancies worldwide. Chemoresistance is a significant clinical problem in pancreatic ductal adenocarcinoma (PDAC) and numerous potential mechanisms have been demonstrated but much remains to be understood. To overcome the existing limitations in PC treatment, newer approaches targeting intrinsic or acquired mechanisms have been found to improve drug therapeutic effectiveness in PC patients. Here, we provide an update of the most recent findings and their implications for clinicians, and attempt to summarize the various aspects of different individualized novel therapies for PC that could most benefit metastatic PDAC patients.

14.
Brain Res Bull ; 153: 191-201, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31472185

RESUMO

The present study aimed to test the hypothesis that propofol (PRO) could exert a neuroprotective effect via inhibiting oxidative stress induced by iron accumulation. Human SH-SY5Y cells were pretreated with ferric citrate (FAC), and then were protected by PRO. Cell viability was measured by MTT method. Iron levels were assayed by ICP-MS. Cell apoptosis was examined by TUNEL and digital holographic technique. Malondialdehyde (MDA), reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) depolarization were measured by MDA, DCFH-DA and JC-1 kits, respectively. The expression of proteins or genes involved in iron metabolism such as ferritin, TfR1, DMT1, Fpn1 and hepcidin, and other apoptosis-related proteins including Bcl2, Bax, Bid, Cox2, IL-6, JAK1 and STAT3 were detected by western blot. Our results showed low concentration of PRO (5 µM) could significantly prevent FAC induced apoptosis via inhibiting oxidative stress and iron accumulation. PRO suppressed the increase of ROS and MDA and decrease of MMP induced by FAC. PRO significantly down-regulated the expression of ferritin and up-regulated the expression of TfR1and Fpn1, but had no effect of DMT1. Furthermore, this effect was not done by PRO chelating iron. Meanwhile, PRO suppressed the inflammatory response through inhibiting IL-6 and Cox2 expression and activating JAK/STAT3 signaling induced by iron overload. In conclusion, here we demonstrated a new antioxidation mechanism of PRO. PRO could protect against nerve cell injury induced by overload of iron through regulating iron metabolism and inhibiting stress oxidative and inflammation reaction pathways by targeting JAK/STAT3 signaling.

15.
Brain Res ; : 146432, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31491422

RESUMO

Therapeutic transplantation of autologous bone marrow mesenchymal stem cells (BMSCs) holds great promise for ischemic stroke, yet the efficacy is negatively impacted by aging. Here, we examined whether hypoxia conditioning could enhance aged human BMSCs-induced neuroprotection via secretome action. Primary cultured mouse neurons were exposed to oxygen glucose deprivation (OGD) to mimic ischemic stroke in vitro, then randomized into a hypoxia conditioned aged human BMSCs-conditioned medium (BMSC-hypoCM) versus normoxia conditioned (BMSC-norCM). After 22 hours of reperfusion, cell viability was significantly increased in neurons treated with BMSC-hypoCM rather than BMSC-norCM. ELISA revealed that hypoxia conditioning enhanced vascular endothelial growth factor (VEGF) release into BMSC-derived CM. Blocking the VEGF receptor negated BMSC-hypoCM-induced protection for neurons against OGD insult. Altogether, our data indicates that hypoxia conditioning improves aged human BMSCs' therapeutic efficacy for neurons with ischemic challenge, in part via promoting secretion of VEGF.

16.
Sci Total Environ ; 697: 134173, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31491636

RESUMO

Low pH requirement is one of the biggest limitations of the application of traditional Fenton and electro-Fenton (EF) process because FeII/FeIII would precipitate at high pH. In this study, a flow-through EF system operated in batch recirculation mode was constructed. Nitrilotriacetic acid (NTA) was used as a chelating agent in the EF system (NTA/EF) to keep iron soluble at high pH values, producing OH by reaction of H2O2 generated in situ with FeIINTA that obtained by the reduction of FeIIINTA at the cathode. This flow-through NTA/EF system accelerated the mass transfer of target molecules to the electrode surface and showed high efficiency for phenol removal at pH 5-8 with rate constants (k) at around 0.26 min-1, higher than that of the batch test (k = 0.15 min-1) and EF process without NTA (k = 0.16 min-1). The influences of aeration rate, current, flow rate, Fe dose, the ratio of NTA to Fe, pH, and initial phenol concentration on the phenol removal were investigated. The system could be used for at least 3 times for phenol removal without obvious efficiency decline. The flow-through NTA/EF system is promising for the removal of organic contaminants in a wide pH range.

17.
Artigo em Inglês | MEDLINE | ID: mdl-31492044

RESUMO

Little is known about the potential interactive effects of heat waves and ambient particulate matter on cardiovascular morbidity. A time-stratified case-crossover design was used to examine whether particulate matter (PM10) modifies the association between heat waves and emergency hospital admissions for six cardiovascular diseases in Greater Sydney, Australia during the warm season for 2001-2013. We estimated and compared the effect of heat waves on high- and low-level PM10 days at lag0-lag2, adjusting for dew-point temperature, ambient ozone, ambient nitrogen dioxide, and public holidays. We also investigated the susceptibility of both younger (0-64 years) and older populations (65 years and above), and tested the sensitivity of three heat wave definitions. Stronger heat wave effects were observed on high- compared to low-level PM10 days for emergency hospital admissions for cardiac arrest for all ages combined, 0-64 years and 65 years and above; conduction disorders for 0-64 years; and hypertensive diseases for all ages combined and 0-64 years. Overall, we found some evidence to suggest that PM10 may modify the association between heat waves and hospital admissions for certain cardiovascular diseases, although our findings largely differed across disease, age group, lag, and heat wave definition.

18.
Anal Chem ; 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31475521

RESUMO

Multifunctional probes integrating accurate multidiagnosis and efficient therapy hold great prospects in biomedical research. However, the sophisticated construction and difficulties in matching the ratios of doses and laser triggers of probes for each modal imaging and therapy still hinder the extensive practice of multifunctional probes in biomedicine. We herein rationally designed an organic dye SY1080 with intrinsic multifunction by introducing both 3,4-ethylenedioxy thiophene (EDOT) and the selenium containing acceptor unit into the backbone to balance the fluorescent brightness and emission wavelength. Under single dose and 808 nm laser irradiation conditions, SY1080 not only carried out NIR-II fluorescence/photoacoustic imaging of real-time and noninvasive tumor delineation with excellent contrast, but also effectively ablated tumors with laser irradiation to perform photothermal therapy under the guidance of dual-modal imaging. These exciting results highlight SY1080 as a multifunctional and universal phototheranostic platform for potential applications.

19.
Sci Total Environ ; 697: 133997, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31479902

RESUMO

To recognize the occurrence and health risk of organophosphate esters (OPEs) in soil of residential areas, a nationwide survey was conducted covering 32 provincial-level administrative regions of China in 2018. Except for triamyl phosphate (TAP), twelve OPEs were detected in residential soil from 89 cities with a total concentration of 1.70-179 ng/g dw, indicating a relatively low contamination level of OPEs in residential soil of China (compared with point source soils and urban soils). Tris(1-chloro-2-propyl) phosphate (TCIPP) was the most abundant OPEs, followed by tris(2-butoxyethyl) phosphate (TBOEP), tris(2-chloroethyl) phosphate (TCEP) and triphenyl phosphate (TPHP), which suggested that TCIPP has been a replacement of TCEP in recent years. An apparent geographical distribution of higher OPEs levels in the Bohai Rim, the Yangtze Delta and Shanxi province than other regions was found. According to the division of administrative levels, the most serious OPEs pollution occurred in prefecture-level city (20.2 ng/g dw), followed by provincial capital (17.9 ng/g dw) and county town (14.1 ng/g dw), which was affected by the usage of OPEs products and behaviors of local residents. The correlation analysis results showed that environmental parameters (total organic carbon (TOC), precipitation and temperature) were not the major factors determining OPEs contamination, while socioeconomic parameters were significantly correlated with OPEs concentration in undeveloped regions/cities with rare industrial source. Although the health risk assessment suggested a negligible risk to local residents (5.92 × 10-7-1.75 × 10-6 for non-carcinogenic risk and 4.82 × 10-12-2.10 × 10-11 for carcinogenic risk), the production and usage of TCIPP and TCEP should be paid more attention due to their relatively high risks. Our study was attempted to provide a nationwide baseline concentration of OPEs in soil of residential areas, which could be used to support further studies.

20.
Int J Neurosci ; : 1-16, 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31487217

RESUMO

Objectives. The objective of this systematic review was to assess the analgesic efficacy of duloxetine (DLX) for fibromyalgia (FM) and find which dosage between 60mg/d DLX and 120mg/d DLX was more suitable for clinical application. Methods. A systematic search through multiple databases (Cochrane Central Register of Controlled Trials (CENTRAL), ProQuest, Pubmed) was conducted from 2000 until March 7, 2019. All steps were performed by two or more independent reviewers. A meta-analysis was performed to report the effects of DLX on pain reduction and its accompanied adverse events. Results. This meta-analysis, including 7 studies with totaling 2642 FM patients, demonstrated that DLX could produce greater pain relief in FM than placebo (standardized mean difference (SMD) -0.26; 95% confidence interval (CI) -0.37 to -0.16). The Risk Ratio (RR) of at least 30% pain relief was 1.31(95%CI 1.19 to 1.44); The RR of at least 50% pain relief was 1.46 (95% CI 1.28 to 1.67). However, the adverse events were more common in patients with DLX than the ones with placebo (RR 1.17, 95%CI 1.12 to 1.23). The subgroup analyses of withdrawal effects demonstrated that 120mg/d DLX had a higher incidence (RR 0.96, 95% CI 0.80 to 1.15) than 60mg/d DLX (RR 0.77, 95% CI 0.63 to 0.93). Conclusions. In general, DLX was a great choice for pain relief in FM. Moreover, 60mg/d DLX produced less withdrawal effects than 120mg/d DLX. Highlight Fibromyalgia(FM) is a chronic condition of unknown etiology, characterized by widespread pain and often associated with other symptoms. Duloxetine (DLX), a serotonin-norepinephrine (noradrenaline) reuptake inhibitor(SNRI), is used to treat FM in many countries. DLX can produce greater pain relief in FM than placebo. DLX can bring about more adverse events than placebo. 60mg/d DLX produce less withdrawal than 120mg/d DLX for FM patients.

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