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1.
J Coll Physicians Surg Pak ; 30(2): 213-215, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32036834

RESUMO

Depending on which part of the physiological pathway is affected by the pathology, jaundice is classified into three categories: pre-hepatic/hemolytic, hepatic/hepatocellular, and post-hepatic/cholestatic. With routine laboratory tests, most cases of jaundice can be etiologically diagnosed. However, exceptions do occur. Here, we present a case of a 14-year girl who presented with intermittent jaundice for one year that could not be diagnosed with a routine protocol. Her laboratory tests showed a moderate impairment of liver function and a positive osmotic fragility test. Computed tomography scan of her upper abdomen revealed multiple gallbladder stones and splenomegaly. With the help of liver pathological examination and exome sequencing, this patient was finally diagnosed as hereditary spherocytosis combined with Gilbert syndrome.

2.
J Invertebr Pathol ; 170: 107323, 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31926972

RESUMO

Bombyx mori nucleopolyhedrosis virus (BmNPV) has always been a great challenge to the development and stability of the sericulture industry. LncRNAs have been reported to play important roles in gene expression regulation, development and immune response but the roles of lncRNAs in BmNPV infection and silkworm-BmNPV interaction are not clear. We used a genome-wide transcriptome analysis to identify the lncRNAs in Bombyx mori cells (BmN cells) and analyzed the differentially expressed lncRNAs, microRNAs and protein-coding genes in silkworm cells with or without BmNPV infection. A total of 13,159 candidate lncRNAs were identified in the BmN cells, among which 4450 lncRNAs were differentially expressed (DE) with 2837 up-regulated and 1613 down-regulated. In addition, 66 differentially expressed miRNAs (DEmiRNAs) and 7448 differentially expressed mRNAs (DEmRNAs) were identified, and DElncRNA-DEmiRNA-DEmRNA regulatory network was constructed. Gene expression was variable in 4973 of predicted lncRNA cis target genes in BmNPV infected cells. KEGG pathway analysis indicated that the target genes of DElncRNAs are enriched in ubiquitin mediated proteolysis, endocytosis and lysosome pathways. qRT-PCR validated the differential expression of several lncRNAs and miRNAs. Our results suggested that DElncRNAs participate in host response to BmNPV infection via interactions with their target genes and miRNAs. Our results will help us to improve our understanding of lncRNA-mediated regulatory roles in BmNPV infection and provide new insights into silkworm-pathogen interactions.

3.
Curr Microbiol ; 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31894373

RESUMO

As a traditional fermented cereal, Inner Mongolian acidic gruel has a unique flavor and rich nutrition, but the microbial diversity of acidic gruel and the microbial differences among the products in different regions have not been reported. The bacterial diversity and the lactic acid bacteria species of 27 types of traditional handmade acidic gruel were evaluated using a combination of MiSeq high-throughput sequencing and PCR-DGGE. All 358,205 high-quality 16S rRNA reads were divided into 25,171 OTUs under the similarity of 97%. Firmicutes, Proteobacteria, and Bacteroidetes were the dominant bacterial phyla, and the core dominant genera were Lactobacillus and Acetobacter with average relative abundances of 64.06% and 24.13%, respectively. The primary genera that caused the differences in the bacterial community structure between the Bayan Nur and Ordos acidic gruel samples were Pseudomonas, Leuconostoc, and Acinetobacter as revealed by redundancy analysis (RDA). PCR-DGGE analyses revealed that the lactic acid bacteria in both the Bayan Nur and Ordos samples of acidic gruel were Lactobacillus (L.) amylolyticus, L. alimentarius, L. fermentum, L. hamsteri, L. helveticus, L. panis, L. plantarum, L. pontis, and Leuconostoc lactis. In addition, L. hamsteri was the core strain detected among all the samples. The results deepened the understanding of the microbial community composition and the diversity of acidic gruel to provide a theoretical basis for the preservation and protection of microbial resources in acidic gruel in the Inner Mongolia area.

4.
PeerJ ; 7: e8031, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31799071

RESUMO

Background: The Mosq-ovitrap (MOT) is currently used for routine surveillance of container-breeding Aedes in China. However, the effectiveness of monitoring Aedes albopictus using the MOT and other mosquito monitoring methods, such as the Ovitrap (OT) and the CO2-light trap (CLT), have not been extensively compared. Moreover, little is known about the spatial-temporal correlations of eggs with adult Ae. albopictus abundance among these three types of traps. Methods: Comparative field evaluation of MOT, OT and CLT for Ae. albopictus monitoring was conducted simultaneously at two city parks and three residential neighborhoods in downtown Shanghai for 8 months from April 21 to December 21, 2017. Results: Significantly more Ae. albopictus eggs were collected from both MOTs and OTs when traps remained in the field for 10 d or 7 d compared with 3 d (MOT: 50.16, 34.15 vs. 12.38 per trap, P < 0.001; OT: 3.98, 2.92 vs. 0.63 per trap, P < 0.001). Egg collections of MOTs were significantly greater than OTs for all three exposure durations (Percent positive: X 2 = 72.251, 52.420 and 51.429, P value all < 0.001; egg collections: t = 8.068, 8.517 and 10.021, P value all <0.001). Significant temporal correlations were observed between yields of MOT and CLT in all sampling locations and 3 different MOT exposure durations (correlation coefficient r ranged from 0.439 to 0.850, P values all < 0.05). However, great variation was found in the spatial distributions of Ae. albopictus density between MOT and CLT. MOT considerably underestimated Ae. albopictus abundances in areas with high Ae. albopictus density (>25.56 per day ⋅ trap by CLT). Conclusion: The MOT was more efficient than the OT in percent positive scores and egg collections of Ae. albopictus. The minimum length of time that MOTs are deployed in the field should not be less than 7 d, as Ae. albopictus collections during this period were much greater than for 3 d of monitoring. MOT considerably underestimated Ae. albopictus abundance in areas with high Aedes albopictus density compared to CLT. In areas with moderate Aedes albopictus densities, MOT results were significantly correlated with CLT catches.

5.
Leuk Lymphoma ; : 1-6, 2019 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-31858854

RESUMO

Hematological toxicity is a common adverse effect of tyrosine kinase inhibitors (TKIs) for the treatment of chronic myeloid leukemia (CML). We retrospectively investigated the incidence of hematological toxicity after TKI administration in 143 CML patients and parameters associated with hematological toxicity. Severe hematological toxicity (grade 3-4) existed in 26 (18.2%) patients. Marrow fibrosis (MF), age, Sokal score, and spleen enlargement were associated with severe hematological toxicity. Further multivariate analysis showed that only MF was an independent predictor. Complete cytogenetic response(CCyR) rates and major molecular response (MMR) rates with grade 3-4 hematological toxicity were 42.3% and 26.9%, respectively, significantly lower than patients with grade 1-2 and without hematological toxicity (p = .032 for CCyR and p = .044 for MMR). Similar results were observed regarding progression-free survival (PFS) and overall survival (OS) (p = .011 for PFS and p = .037 for OS). This study indicated that MF was an independent predictor of severe hematological toxicity of TKIs.

6.
Oncol Lett ; 18(3): 3072-3080, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31452784

RESUMO

Malignant peripheral nerve sheath tumors (MPNSTs) are unusual and aggressive malignant soft-tissue tumors that comprise 5-10% of all soft-tissue sarcomas. Approximately 50% of MPNST cases are associated with neurofibromatosis type-1 (NF-1). As a rare MPNST subset, the epithelioid variant of MPNST (eMPNST) is histologically characterized by the predominant presence of epithelioid tumor cells, and accounts for <5% of all MPNSTs. In addition, eMPNST is rarely associated with NF-1 when compared with conventional MPNST. Although extensive clinicopathological studies have been conducted on eMPNST, clinicians face difficulty when attempting to make an accurate diagnosis. Subsequently, the biological consequences, including recurrence, metastasis and mortality rate in patients with eMPNST remain unclear. The current study presents the case of a 71-year-old woman with eMPNST and a family history of NF-1 in whom tumors had recurred twice on the lower back. A literature search for eMPNSTs was conducted by browsing PubMed and MEDLINE for English-language articles, as well as references from review articles, and revealed 129 published cases. Only 5 cases of eMPNST were associated with NF-1. The studies were retrospectively reviewed and the clinicopathological data of the patients, including tumor site, treatment, follow-up, prognosis, and immunohistochemical positivity were collected.

7.
Bone ; 128: 115039, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31437567

RESUMO

INTRODUCTION: Appendicular fractures are less common in Chinese than Caucasian women. Bone mineral density (BMD) is lower, not higher than in Caucasians because Chinese have smaller appendicular dimensions than Caucasians. However, smaller bones may offset the liability to fracture by being assembled with a more robust microstructure. We hypothesized that Chinese assemble an appendicular skeleton with a thicker, less porous and more mineralized cortex that is less deteriorated in advanced age than in Caucasians. METHODS: We compared anthropometry in 477 Chinese and 278 Caucasian women and compared bone microstructure using high-resolution peripheral quantitative computed tomography in another cohort of 186 Chinese and 381 Caucasian women aged 18 to 86 years, all living in Melbourne, Australia. Trabecular plate (p) and rod (r) bone volume/total volume (BV/TV) were quantified using individual trabecula segmentation (ITS). Bone strength was estimated using micro-finite element analysis (µFEA). RESULTS: Premenopausal Chinese were shorter than Caucasian women, mainly due to shorter leg length. Distal radial total cross sectional area (CSA) was 14.8% smaller (p < 0.001). After adjusting for age and total CSA, Chinese had similar cortical and medullary areas but 0.30 SD lower cortical porosity and 0.27 SD higher matrix mineral density (both p < 0.05). Trabecular plate-to-rod ratio was 0.55 SD higher due to a 0.41 SD higher pBV/TV and 0.36 SD lower rBV/TV (p ranging 0.001 to 0.023). Chinese also had 0.36 SD greater whole bone stiffness and 0.36 SD greater failure load than Caucasians (both p < 0.05). After adjusting for age and total CSA, postmenopausal Chinese had 3.3% smaller cortical area, medullary area was 2.1% larger, cortical porosity was no lower, matrix mineral density and pBV/TV were no higher compared with Caucasians at the distal radius. Whole bone stiffness was 0.39 SD lower and failure load was 0.40 SD lower in Chinese (both p < 0.05). CONCLUSION: Chinese build a more robust skeleton than Caucasians during growth, an advantage not observed in advanced age due to greater bone loss or race-specific secular trends in bone morphology.

8.
J Biomech Eng ; 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31260520

RESUMO

High-resolution peripheral quantitative computed tomography (HRpQCT) is a promising imaging modality that provides in vivo three-dimensional assessment of bone microstructure by scanning fixed regions of the distal radius and tibia. However, how microstructural parameters and mechanical analysis based on these segment scans correlate to whole distal radius and tibia mechanics is not well-characterized. On 26 sets of cadaveric radius and tibia, HRpQCT scans were performed on the standard scan segment, a segment distal to the standard segment, and a segment proximal to the standard segment. Whole distal bone stiffness was determined through mechanical testing. Segment bone stiffness was estimated using linear finite element (FE) analysis based on segment scans. Standard morphological and Individual Trabecula Segmentation (ITS) analyses were used estimate microstructural properties. Significant variations in microstructural parameters were observed among segments at both sites. Correlation to whole distal bone stiffness was moderate for microstructural parameters at the standard segment, but correlation was significantly increased for FE-predicted segment bone stiffness based on standard segment scans. Similar correlation strengths were found between FE-predicted segment bone stiffness and whole distal bone stiffness. Additionally, microstructural parameters at the distal segment had higher correlation to whole distal bone stiffness than at standard or proximal segments. Our results suggest that FE-predicted segment stiffness is a better predictor of whole distal bone stiffness for clinical HRpQCT analysis, and that microstructural parameters at the distal segment is more highly correlated with whole distal bone stiffness than at the standard or proximal segments.

9.
JBMR Plus ; 3(4): e10083, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31044182

RESUMO

We cross-sectionally compared racial differences in bone quality between Chinese women in the United States (US) and Hong Kong (HK) with white women. A total of 514 women were included. We measured bone geometry, mass, microstructure, and stiffness by high-resolution peripheral quantitative computed tomography (HR-pQCT), individual trabecula segmentation (ITS), and microfinite element analysis (µFEA). After adjustment for age and body mass index (BMI), premenopausal Chinese women in the US and HK had smaller bone area but greater radial cortical (Ct.) thickness and Ct. and trabecular (Tb.) volumetric bone mineral density (vBMD) versus white women but did not differ from each other. At the radius, Tb. number was lower and spacing greater in Chinese women from HK and the US versus white women, whereas Chinese women did not differ from each other. Tb. thickness was highest in Chinese women from HK, intermediate in Chinese-Americans, and lowest in white women. Chinese women had more trabecular plates versus white women, leading to greater age- and BMI-adjusted stiffness for premenopausal Chinese women in HK and the US (both p < 0.05) versus white women. Tibial differences were similar in premenopausal women; analogous trends in microstructure were present in postmenopausal women at the tibia, although stiffness did not differ. In contrast, at the radius, cortical, plate-to-rod ratio, and stiffness were similar between postmenopausal HK and white women. Adjusting for age, weight, and height rather than age and BMI tended to reduce differences in bone size and Tb. parameters but accentuate cortical differences such that Chinese premenopausal women in both locations and postmenopausal women from HK had higher stiffness at both skeletal sites compared with white women. Compared with white women, Chinese women in the US and HK have vBMD and microstructural advantages leading to higher or similar mechanical competence in pre- and postmenopausal women, respectively, despite smaller bone size.

10.
Int J Syst Evol Microbiol ; 69(8): 2196-2201, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30900972

RESUMO

Strain HBUAS52074T is a Gram-positive staining, aerobic bacterium that was isolated from Zha-Chili, a traditional fermented food made in China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HBUAS52074T is a member of the genus Lactobacillus and closely related to Lactobacillus nantensis DSM 16982T (98.9 %), Lactobacillus heilongjiangensis DSM 28069T (98.8 %), Lactobacillus formosensis NBRC 1095009T (98.6 %), Lactobacillus futsaii JCM 17355T (98.5 %), Lactobacillus farciminis KCTC 3681T (98.5 %), Lactobacillus musae NBRC 112868T (98.5 %) and Lactobacillus crustorum LMG 23699T (98.4 %). The DNA G+C content is 36.3 mol%. The major cellular fatty acids are C16 : 0 (28.2 %), C18 : 1ω9c (30.5 %) and summed feature 7 (C19 : 1ω6c, and/or C19 : 1ω7c; 14.9 %). Average nucleotide identity and DNA-DNA hybridization (GGDC) values based on genomic comparisons between HBUAS52074T and related type species showed that the bacterium was significantly different from its closest relatives. Using polyphasic taxonomic analysis, we have shown that strain HBUAS52074T is a new species in the genus Lactobacillus, for which we propose the name Lactobacilluszhachilii sp. nov. The type strain is HBUAS52074T (=GDMCC 1.1417T=KCTC 21106T).


Assuntos
/microbiologia , Lactobacillus/classificação , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Ácido Láctico , Lactobacillus/isolamento & purificação , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
11.
Antonie Van Leeuwenhoek ; 112(7): 1087-1094, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30707396

RESUMO

In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1T) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1T is closely related to Mucilaginibacter kameinonensis NBRC 102645T (98.72%), followed by Mucilaginibacter gossypiicola Gh-48T (97.56%) and Mucilaginibacter oryzae DSM 19975T (97.36%). The DNA G + C content of strain RS1T was determined to be 42.80 mol%. ANIb and GGDC values from genomic comparison between the genomes of strain RS1T and the related reference species were less than 95% and 70%, respectively. The major cellular fatty acids (more than 10% of total fatty acids) were identified as iso-C15: 0, C16:0, iso-C17:0-3OH and summed feature 3 (C16: 1ω7c and/or iso-C15:02-OH). The only isoprenoid quinone detected was MK-7. Based on the physiological, genotypic and genomic characteristics, strain RS1T is concluded to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter endophyticus sp. nov. is proposed (type strain RS1T = KCTC 62785T = GDMCC 1.1414T).


Assuntos
Bacteroidetes/isolamento & purificação , Endófitos/isolamento & purificação , Poaceae/microbiologia , Polissacarídeos/metabolismo , Técnicas de Tipagem Bacteriana , Bacteroidetes/classificação , Bacteroidetes/genética , Bacteroidetes/metabolismo , Composição de Bases , DNA Bacteriano/genética , Endófitos/classificação , Endófitos/genética , Endófitos/metabolismo , Ácidos Graxos/química , Ácidos Graxos/metabolismo , Filogenia , Caules de Planta/microbiologia , RNA Ribossômico 16S/genética
12.
J Biomech Eng ; 2019 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-30703208

RESUMO

The high-resolution peripheral quantitative computed tomography (HRpQCT) provides unprecedented visualization of bone microstructure and the basis for constructing patient-specific micro-finite element (µFE) models. Based on HRpQCT images, we have developed a plate rod µFE (PRµFE) method for whole bone segments using individual trabecula segmentation (ITS) and an adaptive cortical meshing technique. In contrast to the conventional voxel approach, the complex microarchitecture of the trabecular compartment is simplified into shell and beam elements based on the trabecular plate-and-rod configuration. Compared to voxel-based µFE models of µCT and mechanical testing, nonlinear analyses of stiffness and yield strength using the HRpQCT-based PRµFE models demonstrated high correlation and accuracy, indicating that the combination of segmented trabecular plate-rod morphology and adjusted cortical mesh adequately captures mechanics of the whole bone segment. Meanwhile, the PRµFE approach reduced model size by nearly 300-fold and shortened computation time for nonlinear analysis from days to within hours, permitting broader clinical application of HRpQCT-based nonlinear µFE modeling. Furthermore, the presented approach was tested using a subset of radius and tibia HRpQCT scans of patients with prior vertebral fracture from a previous study. Results indicated that yield strength for radius and tibia predicted by the PRµFE model was effective in discriminating vertebral fracture subjects from non-fractured controls. In conclusion, the PR µFE model of HRpQCT images accurately predicted mechanics for whole bone segments and can serve as a valuable clinical tool to evaluate musculoskeletal diseases.

13.
Int J Syst Evol Microbiol ; 69(5): 1313-1319, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30801241

RESUMO

Strain HBUAS61001T was isolated from the pickling sauce used to make a traditional fermented food product, datoucai, in China. The strain belonged to the genus Vibrio, but was placed in a clade separate from any known Vibrio species based on the 16S rRNA gene and MLSA results. The genome consisted of two chromosomes: chromosome I was 2 901 449 bp long with a G+C content of 45.4 mol%; and chromosome II was 1 107 930 bp long with a G+C content of 45.5 mol%. The most abundant fatty acids were C16 : 0 (28.1 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c, 29.4 %) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c, 10.1 %). The isoprenoid quinones detected were Q7 and Q8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Strain HBUAS61001T could grow in the presence of up to 17 % NaCl. The calculated average nucleotide identity and in silico DNA-DNA hybridization (GGDC) values of the strain against the closest related type strains were all lower than 95 and 70 %, respectively. Putative genes in the genome associated with survival under high salinity stress were identified. Based on whole genome sequence analysis and phenotypic characteristics, strain HBUAS61001T is a new species in the genus Vibrio, and the name Vibrio zhugei (=GDMCC 1.1416T=KCTC 62784T) is proposed.


Assuntos
/microbiologia , Microbiologia de Alimentos , Filogenia , Vibrio/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vibrio/isolamento & purificação
14.
Int J Syst Evol Microbiol ; 69(1): 139-145, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30614783

RESUMO

In this study, we describe a new genus and species of yeast with high-salt tolerance. The strain was isolated from the pickling sauce used to make Datoucai, a traditional fermented food made from Brassica juncea in Xiangyang, China. Phylogenetic analysis of sequences from the D1/D2 region of the LSU rRNA gene and from the ITS region demonstrated that the strain, reference HBUAS51001T, was most closely related to members of the genera Occultifur and Cystobasidium. However, the greatest similarities between the D1/D2 and ITS nucleotide sequences of strain HBUAS51001T and the most closely related type strains from Occultifur and Cystobasidium were only 91 and 92 %, respectively. This suggests that strain HBUAS51001T does not belong to any currently described species. Strain HBUAS51001T grew readily on media in which xylose was the sole carbon source. The major ubiquinone was Q9. The genome of strain HBUAS51001T was 42.42 Mb with a G+C content of 53.93 mol%. Three candidate genes associated with xylose metabolism were identified. On the basis of genotypic and phenotypic data, strain HBUAS51001T can be considered as both a new species and a new genus, for which the name Halobasidium xiangyangense gen. nov., sp. nov. is proposed. The type strain is HBUAS51001T (=KCTC27810T=GDMCC 2.231T=CCTCC AY 2018002T).


Assuntos
Basidiomycota/classificação , Filogenia , Xilose/metabolismo , Composição de Bases , Basidiomycota/genética , Basidiomycota/isolamento & purificação , China , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Técnicas de Tipagem Micológica , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ubiquinona/química
15.
Sci Total Environ ; 646: 1567-1577, 2019 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-30235641

RESUMO

The activated bio-chars (AB) were successfully synthesized from rice husk by one- and two-step KOH-catalyzed pyrolysis. The two-step pyrolysis can produce the high yields of AB compared to the one-step pyrolysis. Moreover, the yield of AB decreased with the increase of the mass ratio of KOH and char, which had a significant effect on the development of the surface area and porosity of carbon. In particular, the AB derived from the two-step pyrolysis at 750°C (mass ratio of KOH and char was 3) had the highest specific surface area (SBET=2138m2/g) with many micro-porous structures, which was favored for the phenol adsorption. The maximum adsorption capacity of AB2-3-750 reached 201mg/g because of its excellent surface porosity property. The phenol can be efficiently removed from water by only several minutes. The Langmuir model defined well the adsorption isotherm with a high correlation coefficient value, indicating a monolayer adsorption behavior. And the adsorption process defined well with the pseudo-second-order model. The phenol molecules passed into the internal surface via the liquid-film controlled diffusion, so the behavior of phenol adsorption onto the AB was predominantly controlled via the chemisorption. Furthermore, the functional groups on the outer surfaces of AB can attract the phenol molecules onto the internal surfaces via "π-π dispersion interaction" and "donor-acceptor effect".


Assuntos
Carvão Vegetal/química , Hidróxidos/química , Fenol/metabolismo , Compostos de Potássio/química , Adsorção , Modelos Químicos , Oryza , Fenóis
16.
Int J Syst Evol Microbiol ; 69(11): 3344-3349, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30540241

RESUMO

In a survey of endophytic bacteria in Miscanthus sinensis, a strain of Gram-negative, non-endospore-forming, rod-shaped, aerobic bacterium was isolated and designated as RS10T. Phylogenetic analysis based on its 16S rRNA gene sequence showed that the strain RS10T was affiliated with the genus Pedobacter and exhibited the highest sequence similarities to Pedobacter kyungheensis KACC 16221T (97.78 %), Pedobacter roseus KCTC 22187T (97.75 %), Pedobacter humicola KACC 18452T (97.29 %) and Pedobacter soli KACC 14939T (97.23 %). The novel strain contained iso-C15 : 0, iso-C17 : 0-3OH and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as major fatty acids. The only isoprenoid quinone detected in strain RS10T was MK-7. Strain RS10T contains phosphatidylethanolamine and one kind of aminophospholipid as its major polar lipids. The DNA G+C content for RS10T was 39.8 mol%. Based on the results of phenotypic and genomic characterizations, we concluded that strains RS10T represents a novel species of Pedobacter, for which the name Pedobacter miscanthi sp. nov. is proposed. The type strain is RS10T (=KCTC 62786T=GDMCC 1.1415T).


Assuntos
Pedobacter/classificação , Filogenia , Poaceae/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Pedobacter/isolamento & purificação , Fosfolipídeos/química , Caules de Planta/microbiologia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
17.
Int Orthop ; 43(3): 573-577, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-29911277

RESUMO

AIM: To investigate the incidence, risk factors, and the last follow-up recovery status of sciatic and femoral nerve injury among patients who received Bernese peri-acetabular osteotomy (PAO). PATIENTS AND METHODS: The clinical file of 643 consecutive patients who received PAO from June 2012 to June 2016 was retrospectively reviewed. The number of nerve injury patients was calculated and the causes of injury were recorded. RESULTS: The sciatic or femoral nerve injury occurred in eight patients (1.24%), including four sciatic nerve injuries and four femoral nerve injuries. The reasons for sciatic nerve injury included one direct sciatic nerve injury happened at the time when deep osteotomy penetrated the posterior column to cut the nerve trunk at the area where the nerve runs through out of the greater sciatic foramen during quadrilateral bone osteotomy. The other two direct sciatic nerve injuries occurred at the inside pelvis by long drill bit or Kirschner wire drilling before the transverse screw fixation. No direct injury reasons could be found for the remaining five patients with one partial sciatic nerve injury and four femoral nerve palsies. The three patients with direct sciatic nerve injuries were partly recovered at the last follow-up. Full recovery was found in one sciatic nerve injury and four femoral nerve injury patients. CONCLUSION: The sciatic nerve can be injured directly or indirectly during PAO. It is of great importance to understand the risk factors and the precautionary measures of nerve injuries during PAO.

18.
IEEE Trans Image Process ; 28(4): 1625-1635, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30346286

RESUMO

Dilated convolutions support expanding receptive field without parameter exploration or resolution loss, which turn out to be suitable for pixel-level prediction problems. In this paper, we propose multiscale single image super-resolution (SR) based on dilated convolutions. We adopt dilated convolutions to expand the receptive field size without incurring additional computational complexity. We mix standard convolutions and dilated convolutions in each layer, called mixed convolutions, i.e., in the mixed convolutional layer, and the feature extracted by dilated convolutions and standard convolutions are concatenated. We theoretically analyze the receptive field and intensity of mixed convolutions to discover their role in SR. Mixed convolutions remove blind spots and capture the correlation between low-resolution (LR) and high-resolution (HR) image pairs successfully, thus achieving good generalization ability. We verify those properties of mixed convolutions by training 5-layer and 10-layer networks. We also train a 20-layer deep network to compare the performance of the proposed method with those of the state-of-the-art ones. Moreover, we jointly learn maps with different scales from a LR image to its HR one in a single network. Experimental results demonstrate that the proposed method outperforms the state-of-the-art ones in terms of PSNR and SSIM, especially for a large-scale factor.

19.
Conf Proc IEEE Eng Med Biol Soc ; 2019: 1228-1232, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31946114

RESUMO

In this study, we proposed a continuous stroke phase recognition method with lower-limb inertial signals. The aim of the method was to decrease the time needed and to relieve the burdensome manual configurations in the tasks of human underwater motion recognition. The method automatically segmented the data of a period of time into stroke cycles and three sub-phases (propulsion, glide and recovery). K-nearest neighbor algorithm (k-NN) was used as the classifier to train the segmented data and classify the new data on each sample interval. To validate the proposed recognition method, three elite swimmers were recruited. We also designed an wearable sensing system for human underwater motion sensing with inertial measurement units (IMUs). With only data of 5 stroke cycles for training, the recognizer produced accurate recognition results. The average precision across the phases and the subjects was 93.7% and the average recall was 92.6%. We also investigated the time difference of the key stroke events (stroke phase transitions) between the recognized decisions and the reference ones. The average time difference was 66.2 ms, which accounted for the 4.2% of a single stroke phase. The results of the pilot study proved the feasibility of the new method for human aquatic locomotion assistance tasks. Future efforts will be paid in this new direction for more promising results.

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