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1.
Food Chem ; 367: 130701, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34388635

RESUMO

Carotenoids are colored compounds with important physiological functions. The Haida golden scallop, which has an orange adductor muscle, is a carotenoid-enriched variety of scallop Mizuhopecten yessoensis, an important aquaculture shellfish. In this study, we investigated the tissue distribution of the carotenoids, pectenolone and pectenoxanthin, in both Haida golden scallop and normal Yesso scallop. Both carotenoids were detected in all the sampled tissues of the two scallops, except in the adductor muscle of normal scallop. There were significantly more carotenoids in Haida golden scallop than in normal scallop, in the tissues of the mantle, female gonad, kidney, and adductor muscle. Increased carotenoid concentrations were detected in Haida golden scallop adductor muscle during the spring spawning season, indicating the effects of reproduction on muscle carotenoids accumulation. This study was the first systematic investigation of carotenoid distribution in Yesso scallop tissues and will benefit future research on carotenoid accumulation and function in scallops.


Assuntos
Citrus sinensis , Pectinidae , Animais , Carotenoides/metabolismo , Citrus sinensis/metabolismo , Músculo Esquelético/metabolismo , Pectinidae/metabolismo , Estações do Ano , Distribuição Tecidual
2.
Spectrochim Acta A Mol Biomol Spectrosc ; 264: 120300, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34455388

RESUMO

Breast cancer is a major health threat for women. The drug responses associated with different breast cancer subtypes have obvious effects on therapeutic outcomes; therefore, the accurate classification of breast cancer subtypes is critical. Breast cancer subtype classification has recently been examined using various methods, and Raman spectroscopy has emerged as an effective technique that can be used for noninvasive breast cancer analysis. However, the accurate and rapid classification of breast cancer subtypes currently requires a great deal of effort and experience with the processing and analysis of Raman spectra data. Here, we adopted Raman spectroscopy and machine learning techniques to simplify and accelerate the process used to distinguish normal from breast cancer cells and classify breast cancer subtypes. Raman spectra were obtained from cultured breast cancer cell lines, and the data were analyzed by two machine learning algorithms: principal component analysis (PCA)-discriminant function analysis (DFA) and PCA-support vector machine (SVM). The accuracies with which these two algorithms were able to distinguish normal breast cells from breast cancer cells were both greater than 97%, and the accuracies of breast cancer subtype classification for both algorithms were both greater than 92%. Moreover, our results showed evidence to support the use of characteristic Raman spectral features as cancer cell biomarkers, such as the intensity of intrinsic Raman bands, which increased in cancer cells. Raman spectroscopy combined with machine learning techniques provides a rapid method for breast cancer analysis able to reveal differences in intracellular compositions and molecular structures among subtypes.


Assuntos
Neoplasias da Mama , Análise Espectral Raman , Algoritmos , Neoplasias da Mama/diagnóstico , Feminino , Humanos , Aprendizado de Máquina , Análise de Componente Principal , Máquina de Vetores de Suporte
3.
Artigo em Inglês | MEDLINE | ID: mdl-34857467

RESUMO

A new perovskite BaLaMgTaO6:Mn4+ (BLMTO:Mn4+) red phosphor was synthesized for the first time via the high-temperature solid-state method. The emission band of the phosphor ranges from 650 to 750 nm, which matches well with the absorption band of PFR and PR. By doping of Bi3+ and Ca2+ ions in the BLMTO:Mn4+ phosphor, a 4.76-fold enhancement in the luminescence emission intensity was achieved. The optimized BLMTO:0.5%Mn4+, 1.5%Bi3+, 2%Ca2+ phosphor exhibited a high quantum efficiency of 65% and a high color purity of 98.1% with the chromaticity coordinate (CIE) at (0.733, 0.267). Finally, a LED device was fabricated with the BLMTO:0.5%Mn4+, 1.5%Bi3+, 2%Ca2+ phosphor for further agricultural lighting, which emits warm white light with a low color temperature of 3549 K. The result indicates that the BLMTO:Mn4+, Bi3+, Ca2+ phosphors have a potential for applications in agricultural cultivations.

4.
Transl Lung Cancer Res ; 10(10): 3912-3928, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34858781

RESUMO

Background: Female menstrual and reproductive factors, as remarkable indicators of hormone effect, were hypothesized to be associated with lung cancer risk, whereas the existed epidemiological evidence was inconsistent. Our study aims to investigate the association between menstrual and reproductive factors and lung cancer risk based on the Chinese Lung Cancer Screening Program. Methods: This study was based on a large-scale multi-center population cohort across China recruiting individuals aged 40-74 years old between 2013-2018. Cox regression model was applied to estimate the HRs and 95% CIs. Restricted cubic spline (RCS) analysis was used to estimate dose-response relationships and test for nonlinear associations. Results: Among 553,434 female participants, 1,529 incident lung cancer cases were identified with a median follow-up of 3.61 years. With adjustment for multiple covariates and all significant hormonal factors, elevated lung cancer risk was associated with later age (15, or ≥16 years) at menarche (HR =1.27, 95% CI: 1.04-1.56; HR =1.45, 95% CI: 1.19-1.76), later age (25-29, or ≥30 years) at first live birth (HR =1.27, 95% CI: 1.13-1.43; HR =1.23, 95% CI: 1.00-1.51), and benign breast disease history (HR =1.25, 95% CI: 1.10-1.41). For postmenopausal females specifically, surgical menopause (HR =1.62; 95% CI: 1.29-2.05) and other surgeries on the reproductive system (HR =1.19; 95% CI: 1.01-1.40) both appeared to be predictive of elevated lung cancer risk. Concerning age at menopause, a nonlinear association was observed (P-nonlinear =0.0126). Increased lung cancer risk was observed among females with age at menopause especially above 50. Although we observed no significant associations between longer time (≥13 months) of breastfeeding and lung cancer risk among all participants (HR =0.86; 95% CI: 0.71-1.04), significant decreased adenocarcinoma risk (HR =0.65; 95% CI: 0.53-0.81) was noted among nonsmoking females. Conclusions: Our findings add some support for the role of menstrual and reproductive factors in lung carcinogenesis. However, these relationships were complex, and required further investigations addressing the biological mechanisms.

5.
Mol Genet Genomics ; 2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34786636

RESUMO

Brassica juncea is one of a unique vegetable in China, its tumorous stem can be processed into pickle or as fresh vegetable. For a long time, early-bolting as a main factor affects yield and quality of B. juncea, which happens about 15% all year round. As plant specific blue light receptors, FKF1/LKP2 involved in photoperiod flowering. To analyze the expression levels of BjuFKF1/BjuLKP2 and screen their interaction proteins in B. juncea, qRT-PCR and yeast two hybrid assays were recruited. qRT-PCR assays found that the expression levels of BjuFKF1 and BjuLKP2 were up-regulated expressed under both white and blue light. When under different light, BjuFKF1 was significantly increased at vegetative growth stage, but decreased in flowers under blue light. For BjuLKP2, its expression levels did not show significant changes under different light treatment. To investigate interaction proteins, BjuFKF1 and BjuLKP2 were used as bait proteins, and nine potential proteins were screened from yeast library. Yeast two hybrid assays was recruited to further verify their interaction, the results showed that both BjuFKF1 and BjuLKP2 interacted with BjuCOL, BjuCOL3, BjuCOL5, BjuAP2, BjuAP2-1 and BjuSKP1f, only BjuLKP2 interacted with BjuSVP-1 and BjuCDF1 in vivo. In this study, BjuFKF1 and BjuLKP2 were up-regulated expressed under both white and blue light. Yeast two hybrid results verified that BjuFKF1 and BjuLKP2 interacted with six and eight of those nine proteins in vivo, respectively. All of those results will provided reference genes to study BjuFKF1/BjuLKP2 regulated flowering pathway in B. juncea.

6.
Small ; : e2104200, 2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34786839

RESUMO

Many enveloped viruses utilize endocytic pathways and vesicle trafficking to infect host cells, where the acidification of virus-containing endosomes triggers the virus-endosome fusion events. Therefore, simultaneous correlation of intracellular location, local pH, and individual virus dynamics is important for gaining insight into viral infection mechanisms. Here, an imaging approach is developed for spatiotemporal quantification of endosomal acidification on the viral journey in host cells using a fluorescence resonance energy transfer based ratiometric pH sensor consisting of a photostable and high-brightness QD, pH-sensitive fluorescent dyes, and virus-binding proteins. Ratiometric analysis of sensor-based single-virus tracking data enables to dissect a two-step endosomal acidification process during the infection of influenza viruses and elucidates the occurrence of the fission and sorting of virus-containing endosomes to recycling endosomes after initial acidification. This technique should serve as a robust approach for in situ quantification of endosomal acidification on the viral journey.

7.
ACS Appl Mater Interfaces ; 13(45): 53564-53573, 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34726381

RESUMO

Intelligent drug delivery systems (DDSs) that can improve therapeutic outcomes of antitumor agents and decrease their side effects are urgently needed to satisfy special requirements of treatment of malignant tumors in clinics. Here, the fabrication of supramolecular self-assembled amphiphiles based on the host-guest recognition between a cationic water-soluble pillar[6]arene (WP6A) host and a sodium decanesulfonate guest (G) is reported. The chemotherapeutic agent doxorubicin hydrochloride (DOX) can be encapsulated into the formed vesicle (G/WP6A) to construct supramolecular DDS (DOX@G/WP6A). WP6A affords strong affinities to G to avoid undesirable off-target leakage during delivery. Nanoscaled DOX@G/WP6A is capable of preferentially accumulating in tumor tissue via enhanced permeability and retention (EPR) effect. After internalization by tumor cells, the abundant adenosine triphosphate (ATP) binds competitively with WP6A to trigger the disintegration of self-assembled vesicles with the ensuing release of DOX. In vitro and in vivo research confirmed that DOX@G/WP6A is not only able to promote antitumor efficacy but also reduce DOX-related systemic toxicity. The above favorable findings are ascribed to the formation of ternary self-assembly, which profits from the combination of the factors of the EPR effect and the ATP-triggered release.

8.
Free Radic Biol Med ; 2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34767921

RESUMO

Calcium oxalate (CaOx) is the most common component of kidney stones. Oxidative stress, inflammation and autophagy-induced cell death are the major causes of CaOx crystal deposition and CaOx crystal deposition can further lead to kidney injury. Trimethylamine N-oxide (TMAO), a gut microbiota-derived metabolite, plays an important role in the pathogenesis of many diseases, such as atherosclerosis, diabetes and chronic kidney disease, but the effect of TMAO on hyperoxaluria-induced CaOx crystal deposition and kidney injury remains unknown. We hypothesize that TMAO aggravates CaOx crystal deposition via promoting CaOx-mediated cell death. C57Bl/6 mice were given high-oxalate diet as a model of hyperoxaluria. TMAO was provided via drinking water. Serum TMAO levels increased 15 days after CaOx treatment (6.30 ± 0.17 µmol/L vs. 34.65 ± 8.95 µmol/L). High-oxalate diet induced inflammation, CaOx deposition and kidney injury, which TMAO aggravated. In accordance, TMAO intensified high-oxalate diet induced oxidative stress, autophagy and apoptosis. Moreover, TMAO enhanced CaOx crystal adhesion to HK-2 cells and reduced cell viability (from 88.9 ± 1.6% to 75.0 ± 2.7%). Protein kinase R-like endoplasmic reticulum kinase (PERK) may mediate these TMAO effects, as TMAO promoted PERK phosphorylation. Consistently, PERK knockdown alleviated TMAO-evoked CaOx-autophagy, apoptosis and oxidative stress in HK-2 cells. In conclusion, TMAO can aggravate hyperoxaluria-induced kidney injury by triggering the PERK/ROS pathway, which enhances autophagy, apoptosis and inflammation, and facilitates CaOx crystal deposition in renal tubular cells.

9.
Phytochem Anal ; 2021 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-34750870

RESUMO

INTRODUCTION: Chrysanthemums are traditional flowers that originated in China and have high ornamental, economic and medicinal value. They are widely used as herbal remedies and consumed as food or beverages in folk medicine. However, little is known about their metabolic composition. OBJECTIVES: The aims of this work were to determine the metabolic composition of and natural variation among different species of Chrysanthemum and to explore new potential resources for drug discovery and sustainable utilisation of wild Chrysanthemum. METHODS: The metabolomes of Chrysanthemum mongolicum (Ling) Tzvel. and Chrysanthemum rhombifolium H. Ohashi & Yonek. were compared using a widely targeted metabolomics approach based on liquid chromatography-tandem mass spectrometry (LC-MS/MS). RESULTS: In total, 477 metabolites were identified, of which 72 showed significant differences in expression between C. mongolicum and C. rhombifolium, mainly in flavonoids, organic acids and nucleotides. The flavone and flavonol biosynthesis pathway showed significant enrichment among the differentially expressed metabolites. The contents of genkwanin, trigonelline, diosmin, narcissoside, 3,4-dihydroxyphenylacetic acid, linarin, N',N'-p-coumarin, C-hexosyl-tricetin O-pentoside, chrysoeriol, acacetin and kaempferol-3-O-gentiobioside were significantly different between the two species and represent potential biomarkers. CONCLUSION: The types of flavonoid-related metabolites in the flavonoid biosynthesis pathway differed between C. mongolicum and C. rhombifolium. The mechanisms underlying the unique adaptations of these two species to their environments may involve variations in the composition and abundance of flavonoids, organic acids, and nucleotides. These methods are promising to identify functional compounds in Chrysanthemum species and can provide potential resources for drug discovery and the sustainable utilisation of Chrysanthemum plants.

10.
J Hazard Mater ; 424(Pt C): 127321, 2021 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-34741940

RESUMO

The effect of different crystal phases, i.e. spinel phase (CuMn2O4) and amorphous phase (Cu0.2MnOx), was explored in Cu-Mn catalytic ozonation of toluene. The toluene removal efficiency followed the order of Cu0.2MnOx (91.2%) ˃ CuMn2O4 (74.5%) ˃ commercial catalyst Cu0.3MnOx (70.3%) in 130 min, and the higher CO2 yield (67.6%) could be also observed using Cu0.2MnOx. In order to investigate the effect of phases on the toluene degradation pathway, the intermediates and byproducts were identified by DRIFTS, GC-MS, and TOF-SIMS. No obvious difference was observed in the distribution of byproducts, except for the quantities, suggesting the discrepancy of oxidation rate. On the other hand, the catalysts were characterized before and after the ozonation process by TEM, BET, XPS, XRD, EPR, TGA, and TPR. It was proposed that for amorphous catalysts, the oxygen vacancy (Vo) helped the chemisorption of toluene, and adjacent Mn reacted as the main active site for the ozonation process. While, the redox pair of Cu+/Mn4+ and Cu2+/(Mn3+, Mn2+) in the spinel phase plays an important role in the generation of oxygen vacancies for O3 decomposition.

11.
Asian J Surg ; 2021 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-34815143

RESUMO

OBJECTIVE: This study aimed to investigate the clinical effect of the encircling fixation of a transplanted palmaris longus tendon in the treatment of Doyle types II and III mallet finger. METHODS: There were 115 cases of mallet finger deformity with Zone 1 extensor tendon rupture and defect. After debridement by first intention, the tendon bundles of the palmaris longus tendon were used to pass through the subcutaneous tunnel on the volar side of the base of distal phalanx, forming an encircling binding, crossing on the dorsal side. The tail of the tendon was then overlapped with the proximal extensor tendon and sutured. The finger extension position was fixed with plaster for four weeks. If the skin defect could not be closed directly, depending on the size of the skin defect, either a local turndown flap or a pedicled flap was used to cover the wound. RESULTS: The patients were followed up for 3-12 months after the operation. According to Total Active Motion criteria, the clinical effect was excellent in 89 cases, good in 16 cases, acceptable in 7 cases, poor in 2 cases and inferior in 1 case. CONCLUSION: The treatment of Doyle types II and type III mallet finger with the encircling fixation of a transplanted palmaris longus tendon is simple and effective, with a low recurrence rate, few complications, and satisfactory results.

12.
Artigo em Inglês | MEDLINE | ID: mdl-34747102

RESUMO

The activation and oxidization of inert C(sp3 )-H bonds into value-added chemicals affords attractively economic and ecological benefits as well as central challenge in modern chemistry. Inspired by the natural enzymatic transformation, herein, we report a new multiphoton excitation approach to activate the inert C(sp3 )-H bonds and oxygen by integrating the photoinduced electron transfer (PET), ligand-to-metal charge transfer (LMCT) and hydrogen atom transfer (HAT) events together into one metal-organic framework. The well-modified nicotinamide adenine dinucleotide (NAD+ ) mimics oxidized CeIII -OEt moieties to generate CeIV -OEt chromophore and its reduced state mimics NAD. via PET. The in situ formed CeIV -OEt moiety triggers a LMCT excitation to form the alkoxy radical EtO. , abstracts a hydrogen atom from the C(sp3 )-H bond, accompanying the recovery of CeIII -OEt and the formation of alkyl radicals. The formed NAD. activates oxygen to regenerate the NAD+ for next recycle, wherein, the activated oxygen species interacts with the intermediates for the oxidization functionalization, paving a catalytic avenue for developing scalable and sustainable synthetic strategy.

13.
J Nutr Biochem ; : 108908, 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34801687

RESUMO

Succinic acid widely exists in foods and is used as a food additive. Succinate not only serves as an energy substrate, but also induces protein succinylation. Histone succinylation activates gene transcription. The brown adipose tissue (BAT) is critical for prevention of obesity and metabolic dysfunction, and the fetal stage is pivotal for BAT development. Up to now, the role of maternal succinate supplementation on fetal BAT development and offspring BAT function remains unexamined. To test, female C57BL/6J mice (2-month-old) were separated into two groups, received with or without 0.5% succinic acid in drinking water during gestation and lactation. After weaning, female offspring were challenged with high fat diet (HFD) for 12 weeks. Newborn, female weanling, and HFD female offspring mice were analyzed. For neonatal and weaning mice, the BAT weight relative to the whole body weight was significantly increased in the succinate group. The expression of PGC-1α, a key transcription co-activator promoting mitochondrial biogenesis, was elevated in BAT of female neonatal and offspring born to succinate-fed dams. Consistently, maternal succinate supplementation enhanced thermogenesis and the expression of thermogenic genes in offspring BAT. Additionally, maternal succinate supplementation protected female offspring against HFD-induced obesity. Furthermore, in C3H10T1/2 cells, succinate supplementation promoted PGC-1α expression and brown adipogenesis. Mechanistically, succinate supplementation increased permissive histone succinylation and H3K4me3 modification in the Ppargc1a promoter, which correlated with the higher expression of Ppargc1a. In conclusion, maternal succinate supplementation during pregnancy and lactation enhanced fetal BAT development and offspring BAT thermogenesis, which prevented HFD-induced obesity and metabolism dysfunction in offspring.

14.
Opt Express ; 29(22): 34986-35002, 2021 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-34808945

RESUMO

Analytical formulas for the cross-spectral density matrix of a partially coherent radially polarized Laguerre-Gaussian vortex (PCRPLGV) beam in anisotropic atmospheric turbulence are derived based on the extended Huygens-Fresnel principle. The evolution laws of statistical properties of a PCRPLGV beam in turbulence, such as the average intensity, degree of coherence (DOC) and degree of polarization (DOP), are investigated in detail. It is found that the atmospheric turbulence induces degeneration of the intensity distribution of a PCRPLGV beam on propagation, and some new properties, such as self-shaping and self-rotating, will appear on propagation due to vortex phase. In addition, in order to verify our theoretical results, we combined the complex screen method and multi-phase screens method to simulate the propagation of PCRPLGV beam in atmospheric turbulence. And the studies indicate that the simulation results are consistent with the theoretical predictions. Our results will be useful in some potential applications, such lidar detection, remote sensing and free-space optical communications.

15.
Artigo em Inglês | MEDLINE | ID: mdl-34774322

RESUMO

The metal oxides-based chemiresitive gas sensors have attracted enormous interest because of their exellent sensing perforamcnes, which have emerged as very promising candidates for gas monitoring. However, from the view of organophosphorus compounds detection, a unique combination of low detection limit and fast respons/recovery rate remainschallenging. Herein, the synersgitic effects of oxygen vacancy engineering and surface gold decoration enabling excellent sensing performances for detection of dimethyl methyl phosphonate (DMMP, a typical organophosphorus) is reported. To demonstrate the proof of concept, Au nanoparticles (NPs) decorated oxygen vacancy-enriched SnO2 hybrids (designated as Au-O-SnO2) were designedas sensing materials, where the O-SnO2 samples were fabricated by introduction of oxygen vacancies onto commercial SnO2 through organometallic chemistry-assisted approach using (CH3)2SnCl2 as precursor, followed by deposition of Au NPs by an in-situ reduction routine. After optimizing Au NPs content in hybrids (1 wt%, 3 wt%, 5 wt% and 7 wt%), O-SnO2 decorated with 5 wt% Au NPs (designated as Au-O-SnO2-5) exhibits excellent DMMP sensing performances, such as, an enhanced recoverable response of 1.67 to 680 ppb DMMP, low detection limit of 4.8 ppb, shortresponse time of 26 s and recoverytime of 32 s, as well as good selectivity, which are much better than that of commercial SnO2 (C-SnO2) and O-SnO2, and Au NPs decorated C-SnO2. Based on the detailed investigation, the enhanced DMMP sensing performances of Au-O-SnO2 hybrids can be mainly ascribed to the synergistic effect of increasing surface active sites induced by oxygen vacancies, the chemical and electronic sensitization of Au NPs. As a result, Au-O-SnO2-5 hybrids display relatively low activation energy of 24.11 kJ/mol for DMMP oxidization, which is lower than that of O-SnO2 (35.54 kJ/mol). Our results provide a feasible method for boosting sensing performances for DMMP detection, paving new way for fabrication of metal oxides-based gas sensors for rapid detection of trace organic compounds with complexed structures.

16.
Heart Rhythm ; 2021 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-34775069

RESUMO

BACKGROUND: Pulmonary vein isolation (PVI) for paroxysmal atrial fibrillation (AF) is associated with a non-negligible long-term recurrence rate. OBJECTIVES: The aim of this study was to investigate whether PVI combined with six short ablation lines on the PVI circumferences (PVI+6L group) yields higher success rates than PVI alone (PVI group). METHODS: In this multicenter, single-blind, randomized trial, a total of 390 patients with paroxysmal AF were randomly assigned into PVI group (n=193) or PVI+6L group (n=197). The primary endpoint was freedom from AF/atrial tachycardia recurrence between 91 and 365 days. The secondary endpoints included AF burden, procedural parameters, and complications. RESULTS: Freedom from atrial tachyarrhythmia was achieved in in 160 of 197 patients (81.2%) in the PVI+6L group and 142 of 193 patients (73.6%) in the PVI group (hazard ratio, 0.61; 95% confidence interval, 0.39 to 0.97; P=0.040). Mean AF burden tended to be lower in PVI+6L group as compared to PVI group (1.95% vs. 0.53%, P=0.097). Procedure and ablation time were slightly longer in the PVI+6L group than in the PVI group (130 ± 25 vs. 121 ± 28 min, P=0.002, and 46 ± 14 vs. 41 ± 16 min, P=0.001, respectively) while X-ray exposure was similar (60 ± 54 vs. 61 ± 60 s, P=0.964). Complications occurred in three patients (1.6%) in the PVI group and in three patients (1.5%) in the PVI+6L group. CONCLUSIONS: In patients with paroxysmal AF undergoing catheter ablation, adding six short ablation lines on the PVI circumferences could reduce recurrence rate of AF.

17.
Viruses ; 13(11)2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34835006

RESUMO

The Madin-Darby Canine Kidney (MDCK) cell line is among the most commonly used cell lines for the production of influenza virus vaccines. As cell culture-based manufacturing is poised to replace egg-based processes, increasing virus production is of paramount importance. To shed light on factors affecting virus productivity, we isolated a subline, H1, which had twice the influenza virus A (IAV) productivity of the parent (P) through cell cloning, and characterized H1 and P in detail on both physical and molecular levels. Transcriptome analysis revealed that within a few hours after IAV infection, viral mRNAs constituted over one fifth of total mRNA, with several viral genes more highly expressed in H1 than P. Functional analysis of the transcriptome dynamics showed that H1 and P responded similarly to IAV infection, and were both subjected to host shutoff and inflammatory responses. Importantly, H1 was more active in translation and RNA processing intrinsically and after infection. Furthermore, H1 had more subdued inflammatory and antiviral responses. Taken together, we postulate that the high productivity of IAV hinges on the balance between suppression of host functions to divert cellular resources and the sustaining of sufficient activities for virus replication. Mechanistic insights into virus productivity can facilitate the process optimization and cell line engineering for advancing influenza vaccine manufacturing.

18.
Small ; : e2104567, 2021 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-34837314

RESUMO

In vivo fluorescence imaging can perform real-time, noninvasive, and high spatiotemporal resolution imaging to accurately obtain the dynamic biological information in vivo, which plays significant roles in the early diagnosis and treatment of cancer. However, traditional in vivo fluorescence imaging usually operates in the visible and near-infrared (NIR)-I windows, which are severely interfered by the strong tissue absorption, tissue scattering, and autofluorescence. The emergence of NIR-II imaging at 1000-1700 nm significantly breaks through the imaging limitations in deep tissues, due to less tissue scattering and absorption. Benefiting from the outstanding optical properties of NIR-II quantum dots (QDs), such as high brightness and good photostability, in vivo fluorescence imaging exhibits excellent temporal-spatial resolution and large penetration depth, and QDs have become a kind of promising fluorescent biomarkers in the field of in vivo fluorescence imaging. Herein, the authors review NIR-II QDs from preparation to modification, and summarize recent applications of NIR-II QDs, including in vivo imaging and imaging-guided therapies. Finally, they discuss the special concerns when NIR-II QDs are shifted from in vivo imaging applications to further in-depth applications.

19.
Neoplasia ; 23(12): 1227-1239, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34768109

RESUMO

Sorafenib is a first-line molecular-target drug for advanced hepatocellular carcinoma (HCC), and reducing sorafenib resistance is an important issue to be resolved for the clinical treatment of HCC. In the current study, we identified that ABCC5 is a critical regulator and a promising therapeutic target of acquired sorafenib resistance in human hepatocellular carcinoma cells. The expression of ABCC5 was dramatically induced in sorafenib-resistant HCC cells and was remarkably associated with poor clinical prognoses. The down-regulation of ABCC5 expression could significantly reduce the resistance of sorafenib to HCC cells. Importantly, activation of PI3K/AKT/NRF2 axis was essential for sorafenib to induce ABCC5 expression. ABCC5 increased intracellular glutathione (GSH) and attenuated lipid peroxidation accumulation by stabilizing SLC7A11 protein, which inhibited ferroptosis. Additionally, the inhibition of ABCC5 enhanced the anti-cancer activity of sorafenib in vitro and in vivo. These findings demonstrate a novel molecular mechanism of acquired sorafenib resistance and also suggest that ABCC5 is a new regulator of ferroptosis in HCC cells.

20.
Anal Methods ; 13(46): 5564-5572, 2021 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-34780584

RESUMO

Level 3 details play essential roles in practical latent fingerprint (LFP) identification. To reliably extract reproducible and identifiable level 3 features, high-resolution images of fingerprints with adequate quality are required. Conventional methods for acquiring level 3 details often involve specific pretreatment, intricate peripheral, leading to time-consuming analysis. Herein, we simply used water to develop the sebaceous LFPs deposited on nitrocellulose (NC) membranes with only one step, and then the high-resolution (2048 pixels per inch) optical micrographs were captured to reflect the live fingertip with high fidelity. From the pictures, level 3 features, including all dimensional attributes of the ridges and pores such as number, size, location, shape, and edge contour can be extracted accurately and reproducibly. Among them, qualitative features (the structures of ridge edges) and several quantitative characteristics (the number and the relative location of sweat pores) exhibit good reproducibility. Remarkably, we proposed a new parameter termed "frequency distribution of the distance between adjacent sweat pores", short form "FDDasp", which was further proved highly identifiable in different individuals, enabling the successful distinguishment between two fragmentary fingerprints with similar level 2 structures. We believe that this methodology provides a fast and quantitative analytical paradigm for latent fingerprint identification at level 3 details.

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