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1.
Artigo em Inglês | MEDLINE | ID: mdl-33571038

RESUMO

Background: Laryngeal squamous cell carcinoma (LSCC) has poor prognosis, and the mechanism underlying the pathogenesis of LSCC remains unclear. Recently, study has shown that long nonprotein coding RNA ferritin heavy chain 1 pseudogene 3 (FTH1P3) plays a crucial role in tumor pathogenesis. This study aimed to explore the potential role of FTH1P3 in LSCC. Materials and Methods: The expression of E2F1 and FTH1P3 in LSCC was analyzed by quantitative real time-polymerase chain reaction assay. The direct targets of FTH1P3 and miR-377-3p were predicted, followed by functional validation. The functional role of FTH1P3 was investigated in AMC-HN-8 and TU686 cells using 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide assays and the measurement of glucose uptake and L-lactate production. Results: Their results found that overexpression of FTH1P3 promoted, but knockdown of FTH1P3 suppressed cell viability and glycolysis in LSCC cells. Besides, upregulated FTH1P3 was associated with increased E2F1 expression in LSCC patients. E2F1 was proved to induce FTH1P3 expression in LSCC cells. Mechanically, FTH1P3 modulated miR-377-3p expression by targeting miR-377-3p. Interestingly, LDHA was identified to be a target of miR-377-3p, and FTH1P3 promoted LDHA expression by suppressing miR-377-3p. In addition, knockdown of FTH1P3 mitigated E2F1-induced cell viability and glycolysis through miR-377-3p/LDHA in AMC-HN-8 cells. More importantly, knockdown of E2F1 inhibited tumor growth and FTH1P3 expression in vivo. Conclusion: In conclusion, these findings revealed that E2F1-induced FTH1P3 promoted cell viability and glycolysis through miR-377-3p/LDHA axis in LSCC, which could provide a promising novel strategy for LSCC treatment.

2.
Artigo em Inglês | MEDLINE | ID: mdl-33560822

RESUMO

Polyanion-type Na3V2(PO4)3 (NVP) is an overwhelmingly attractive cathode material for sodium-ion batteries (SIBs) because of its high structural stability and fast Na+ mobility. However, its practical application is strongly plagued by either nanoscale particle size or poor rate performance. Herein, a micro/nanocomposite NVP cathode with a hierarchical porous structure is proposed to solve the problem. The microscale NVP material assembled by interconnected nanoflakes with N-doped carbon coating that is capable of simultaneously providing fast carrier transmission dynamics and outstanding structural integrity exhibits precedent sodium-storage behavior. It delivers a superior rate capability (79.1 mAh g-1 at 200C) and excellent long-life cycling (capacity retention of 73.4% after 10 000 cycles at 100C). Remarkably, a pouch-type sodium-ion full cell consisting of the as-obtained NVP cathode and a hard carbon anode demonstrates the gravimetric energy density as high as 212 Wh kg-1 and an exceptional rate performance (71.8 mAh g-1 at 10C). Such structural design of fabricating micro/nanocomposite electrode materials is expected to accelerate the practical applications of SIBs for large-scale energy storage.

3.
Nat Nanotechnol ; 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33603238

RESUMO

Many nanoscale biomaterials fail to reach the clinical trial stage due to a poor understanding of the fundamental principles of their in vivo behaviour. Here we describe the transport, transformation and bioavailability of MoS2 nanomaterials through a combination of in vivo experiments and molecular dynamics simulations. We show that after intravenous injection molybdenum is significantly enriched in liver sinusoid and splenic red pulp. This biodistribution is mediated by protein coronas that spontaneously form in the blood, principally with apolipoprotein E. The biotransformation of MoS2 leads to incorporation of molybdenum into molybdenum enzymes, which increases their specific activities in the liver, affecting its metabolism. Our findings reveal that nanomaterials undergo a protein corona-bridged transport-transformation-bioavailability chain in vivo, and suggest that nanomaterials consisting of essential trace elements may be converted into active biological molecules that organisms can exploit. Our results also indicate that the long-term biotransformation of nanomaterials may have an impact on liver metabolism.

4.
Artigo em Inglês | MEDLINE | ID: mdl-33434002

RESUMO

In this work, we propose a sulfonated poly (ether ether ketone) (SPEEK) composite proton-conductive membrane based on a 3-(1-hydro-imidazolium-3-yl)-propane-1-sulfonate (Him-pS) additive to break through the trade-off between conductivity and selectivity of a vanadium redox flow battery (VRFB). Specifically, Him-pS enables an oriented distribution of the SPEEK matrix to construct highly conductive proton nanochannels throughout the membrane arising from the noncovalent interaction. Moreover, the "acid-base pair" effect from an imidazolium group and a sulfonic group further facilitates the proton transport through the nanochannels. Meanwhile, the structure of the acid-base pair is further confirmed based on density functional theory calculations. Material and electrochemical characterizations indicate that the nanochannels with a size of 16.5 nm are vertically distributed across the membrane, which not only accelerate proton conductivity (31.54 mS cm-1) but also enhance the vanadium-ion selectivity (39.9 × 103 S min cm-3). Benefiting from such oriented proton-conductive nanochannels in the membrane, the cell delivers an excellent Coulombic efficiency (CE, ≈ 98.8%) and energy efficiency (EE, ≈ 78.5%) at 300 mA cm-2. More significantly, the cell maintains a stable energy efficiency over 600 charge-discharge cycles with only a 5.18% decay. Accordingly, this work provides a promising fabrication strategy for a high-performance membrane of VRFB.

5.
Theranostics ; 11(6): 2534-2549, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33456558

RESUMO

Rationale: Most contemporary cancer therapeutic paradigms involve initial imaging as a treatment roadmap, followed by the active engagement of surgical operations. Current approved intraoperative contrast agents exemplified by indocyanine green (ICG) have a few drawbacks including the inability of pre-surgical localization. Alternative near-infrared (NIR) dyes including IRDye800cw are being explored in advanced clinical trials but often encounter low chemical yields and complex purifications owing to the asymmetric synthesis. A single contrast agent with ease of synthesis that works in multiple cancer types and simultaneously allows presurgical imaging, intraoperative deep-tissue three-dimensional visualization, and high-speed microscopic visualization of tumor margins via spatiotemporally complementary modalities would be beneficial. Methods: Due to the lack of commercial availability and the absence of detailed synthesis and characterization, we proposed a facile and scalable synthesis pathway for the symmetric NIR water-soluble heptamethine sulfoindocyanine IRDye78. The synthesis can be accomplished in four steps from commercially-available building blocks. Its symmetric resonant structure avoided asymmetric synthesis problems while still preserving the benefits of analogous IRDye800cw with commensurable optical properties. Next, we introduced a low-molecular-weight protein alpha-lactalbumin (α-LA) as the carrier that effectively modulates the hepatic clearance of IRDye78 into the preferred renal excretion pathway. We further implemented 89Zr radiolabeling onto the protein scaffold for positron emission tomography (PET). The multimodal imaging capability of the fluorophore-protein complex was validated in breast cancer and glioblastoma. Results: The scalable synthesis resulted in high chemical yields, typically 95% yield in the final step of the chloro dye. Chemical structures of intermediates and the final fluorophore were confirmed. Asymmetric IRDye78 exhibited comparable optical features as symmetric IRDye800cw. Its well-balanced quantum yield affords concurrent dual fluorescence and optoacoustic contrast without self-quenching nor concentration-dependent absorption. The NHS ester functionality modulates efficient covalent coupling to reactive side-chain amines to the protein carrier, along with desferrioxamine (DFO) for stable radiolabeling of 89Zr. The fluorophore-protein complex advantageously shifted the biodistribution and can be effectively cleared through the urinary pathway. The agent accumulates in tumors and enables triple-modal visualization in mouse xenograft models of both breast and brain cancers. Conclusion: This study described in detail a generalized strategic modulation of clearance routes towards the favorable renal clearance, via the introduction of α-LA. IRDye78 as a feasible alternative of IRDye800cw currently in clinical phases was proposed with a facile synthesis and fully characterized for the first time. This fluorophore-protein complex with stable radiolabeling should have great potential for clinical translation where it could enable an elegant workflow from preoperative planning to intraoperative deep tissue and high-resolution image-guided resection.

6.
Planta ; 253(2): 25, 2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33404767

RESUMO

MAIN CONCLUSION: The presence of Bacillus cereus plays a key role in clubroot suppression and improves plant biomass in pak choi. B. cereus is reported for the first time as a novel biocontrol agent against clubroot. Plasmodiophora brassicae Woronin causes a devastating infectious disease known as clubroot that is damaging to cruciferous vegetables. This study aimed to isolate beneficial bacteria from the rhizosphere soil of pak choi (Brassica campestris sp. chinensis) and to evaluate the ability of the isolate to reduce the severity of clubroot. Strains obtained from the rhizosphere of symptomless pak choi were first selected on the basis of their germination inhibition rate and effects on the viability of P. brassicae resting spores. Eight bacterial isolates had inhibitory effects against the resting spores of clubroot causing pathogen. However, MZ-12 showed the highest inhibitory effect at 73.4%. Inoculation with MZ-12 enhanced the plant biomass relative to plants grown without MZ-12 as well as P. brassicae infected plants. Furthermore, enhanced antioxidant enzymatic activities were observed in clubroot-infected plants during bacterial association. Co-inoculation of the plant with both P. brassicae and MZ-12 resulted in a 64% reduction of gall formation in comparison to plants inoculated with P. brassicae only. Three applications of MZ-12 to plants infected with P. brassicae at 7, 14 and 21 days after seeding (DAS) were more effective than one application and repressed root hair infection. According to 16S rDNA sequence analysis, strain MZ-12 was identified as had a 100% sequence similarity with type strain Bacillus cereus. The findings of the present study will facilitate further investigation into biological mechanisms of cruciferous clubroot control.

7.
Transfus Apher Sci ; : 103041, 2021 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-33455877

RESUMO

BACKGROUND: Anti-allergic agents (e.g. dexamethasone, chlorpheniramine or promethazine) are commonly administered to patients prior to blood product transfusions. However, the use of these agents is largely experience-based instead of evidence-based. This meta-analysis aimed to explore the evidence behind using anti-allergic agents to attenuate transfusion reactions. MATERIALS AND METHODS: The Pubmed, EMBASE, Cochrane Library, Wanfang, Chinese National Knowledge Infrastructure (CNKI), and Chinese Biomedical literature (CMB) databases were all queried for related articles. Data from groups treated with and without anti-allergic agents were collected for meta-analysis using RevMan 5.3. Baseline characteristics and univariate statistics between groups were compared using SPSS 19.0. RESULTS: Eight eligible articles (six case control studies and two randomized controlled trials, all with high risks of bias) were identified (22060 total cases). Administered anti-allergic agents in these studies only included dexamethasone, chlorpheniramine or promethazine. Baseline characteristics showed no significant age or gender differences between treatment or control groups. There were no significant differences between the pooled experimental or control groups (for each of the three medications) in terms of fever, pruritis, rash, airway spasm or overall transfusion reaction rates. CONCLUSION: There is no evidence that dexamethasone, chlorpheniramine or promethazine can prevent transfusion reactions. Avoiding the arbitrary use of such anti-allergic agents before blood transfusions may potentially avoid needless adverse drug reactions.

8.
Am J Hum Genet ; 108(2): 337-345, 2021 02 04.
Artigo em Inglês | MEDLINE | ID: mdl-33434492

RESUMO

Mayer-Rokitansky-Küster-Hauser syndrome (MRKHS) is associated with congenital absence of the uterus, cervix, and the upper part of the vagina; it is a sex-limited trait. Disrupted development of the Müllerian ducts (MD)/Wölffian ducts (WD) through multifactorial mechanisms has been proposed to underlie MRKHS. In this study, exome sequencing (ES) was performed on a Chinese discovery cohort (442 affected subjects and 941 female control subjects) and a replication MRKHS cohort (150 affected subjects of mixed ethnicity from North America, South America, and Europe). Phenotypic follow-up of the female reproductive system was performed on an additional cohort of PAX8-associated congenital hypothyroidism (CH) (n = 5, Chinese). By analyzing 19 candidate genes essential for MD/WD development, we identified 12 likely gene-disrupting (LGD) variants in 7 genes: PAX8 (n = 4), BMP4 (n = 2), BMP7 (n = 2), TBX6 (n = 1), HOXA10 (n = 1), EMX2 (n = 1), and WNT9B (n = 1), while LGD variants in these genes were not detected in control samples (p = 1.27E-06). Interestingly, a sex-limited penetrance with paternal inheritance was observed in multiple families. One additional PAX8 LGD variant from the replication cohort and two missense variants from both cohorts were revealed to cause loss-of-function of the protein. From the PAX8-associated CH cohort, we identified one individual presenting a syndromic condition characterized by CH and MRKHS (CH-MRKHS). Our study demonstrates the comprehensive utilization of knowledge from developmental biology toward elucidating genetic perturbations, i.e., rare pathogenic alleles involving the same loci, contributing to human birth defects.


Assuntos
Transtornos 46, XX do Desenvolvimento Sexual/genética , Anormalidades Congênitas/genética , Ductos Paramesonéfricos/anormalidades , Ductos Paramesonéfricos/crescimento & desenvolvimento , Mutação , Ductos Mesonéfricos/crescimento & desenvolvimento , Adulto , Proteína Morfogenética Óssea 4/genética , Proteína Morfogenética Óssea 7/genética , Códon sem Sentido , Feminino , Estudos de Associação Genética , Pleiotropia Genética , Proteínas Homeobox A10/genética , Proteínas de Homeodomínio/genética , Humanos , Fator de Transcrição PAX8/genética , Herança Paterna , Penetrância , Proteínas com Domínio T/genética , Fatores de Transcrição/genética , Proteínas Wnt/genética , Ductos Mesonéfricos/anormalidades
9.
J Mol Graph Model ; 102: 107777, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33130390

RESUMO

Conotoxins are a group of cysteine-rich, neurotoxic peptides isolated from the venom of marine cone snails. MfVIA is a member of the µO-conotoxin family, and acts as an inhibitor of subtype 1.8 voltage-gated sodium ion channels (NaV1.8). The unique selectivity of MfVIA as an inhibitor of NaV1.8 makes it an ideal peptide for elucidation of the physiological functions of this voltage-gated ion channel. Previous experimental studies of point mutations of MfVIA showed that the double mutant [E5K,E8K] exhibited greater activity at NaV1.8 relative to the wild-type toxin. The present study employs molecular dynamics (MD) simulations to examine the effects of various mutations at these key residues (E5 and E8) on the structure and dynamics of MfVIA. Five double mutants were studied, in which the positions 5 and 8 residues were mutated to amino acids with a range of different physicochemical properties, namely [E5A,E8A], [E5D,E8D], [E5F,E8F], [E5K,E8K], and [E5R,E8R]. Except for [E5D,E8D], all of the mutants tend to show decreased contacts at the N-terminus owing to the loss of the R1-E5 salt bridge relative to that of the wild-type, which subsequently lead to greater exposure and flexibility of the N-terminus for most of the mutant peptides studied, potentially rendering them more able to interact with other species, including NaV1.8. Molecular docking studies of the peptides to NaV1.8 via different binding mechanisms suggest that the [E5R, E8R] mutant may be especially worthy of further investigation owing to its predicted binding mode, which differs markedly from those of the other peptides in this study.

10.
Entropy (Basel) ; 22(4)2020 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-33286185

RESUMO

Sample entropy (SampEn) is widely used for electrocardiogram (ECG) signal analysis to quantify the inherent complexity or regularity of RR interval time series (i.e., heart rate variability (HRV)), with the hypothesis that RR interval time series in pathological conditions output lower SampEn values. However, ectopic beats can significantly influence the entropy values, resulting in difficulty in distinguishing the pathological situation from normal situations. Although a theoretical operation is to exclude the ectopic intervals during HRV analysis, it is not easy to identify all of them in practice, especially for the dynamic ECG signal. Thus, it is important to suppress the influence of ectopic beats on entropy results, i.e., to improve the robustness and stability of entropy measurement for ectopic beats-inserted RR interval time series. In this study, we introduced a physical threshold-based SampEn method, and tested its ability to suppress the influence of ectopic beats for HRV analysis. An experiment on the PhysioNet/MIT RR Interval Databases showed that the SampEn use physical meaning threshold has better performance not only for different data types (normal sinus rhythm (NSR) or congestive heart failure (CHF) recordings), but also for different types of ectopic beat (atrial beats, ventricular beats or both), indicating that using a physical meaning threshold makes SampEn become more consistent and stable.

11.
Entropy (Basel) ; 22(5)2020 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-33286292

RESUMO

Due to the wide inter- and intra-individual variability, short-term heart rate variability (HRV) analysis (usually 5 min) might lead to inaccuracy in detecting heart failure. Therefore, RR interval segmentation, which can reflect the individual heart condition, has been a key research challenge for accurate detection of heart failure. Previous studies mainly focus on analyzing the entire 24-h ECG recordings from all individuals in the database which often led to poor detection rate. In this study, we propose a set of data refinement procedures, which can automatically extract heart failure segments and yield better detection of heart failure. The procedures roughly contain three steps: (1) select fast heart rate sequences, (2) apply dynamic time warping (DTW) measure to filter out dissimilar segments, and (3) pick out individuals with large numbers of segments preserved. A physical threshold-based Sample Entropy (SampEn) was applied to distinguish congestive heart failure (CHF) subjects from normal sinus rhythm (NSR) ones, and results using the traditional threshold were also discussed. Experiment on the PhysioNet/MIT RR Interval Databases showed that in SampEn analysis (embedding dimension m = 1, tolerance threshold r = 12 ms and time series length N = 300), the accuracy value after data refinement has increased to 90.46% from 75.07%. Meanwhile, for the proposed procedures, the area under receiver operating characteristic curve (AUC) value has reached 95.73%, which outperforms the original method (i.e., without applying the proposed data refinement procedures) with AUC of 76.83%. The results have shown that our proposed data refinement procedures can significantly improve the accuracy in heart failure detection.

12.
J Proteomics ; : 104071, 2020 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-33307251

RESUMO

Delivery mode is recognized as an important determinant of gut microbiota composition. Vaginally delivered infants were colonized by maternal vaginal and fecal microbiota, while those delivered by cesarean section were colonized by environmental microorganisms. To reveal differences induced by delivery mode, we determined fecal microbiota and fecal metabolome from 60 infants in Northeast China region. Bacterial gene sequence analysis showed that the feces of vaginally delivered infants had the highest abundance of Bifidobacterium, Lactobacillus, Bacteroides and Parabacteroides, while the feces of cesarean section delivered infants were more enriched in Klebsiella. LC-MS-based metabolomics data demonstrated that the feces of vaginally delivered infants were associated with high abundance of DL-norvaline and DL-citrulline, while the feces of cesarean section delivered infants were abundant in trans-vaccenic acid and cis-aconitic acid. Moreover, the feces of vaginally delivered infants was significantly in positive correlation with tryptophan metabolism and pyruvate metabolism, however, the feces of cesarean section delivered infants was positively correlated with ABC transporters. Collectively, our study demonstrated that gut microbiota and metabolite profiles were significantly different between vaginally delivered and cesarean section delivered infants, and provided the theoretical basis for restoring the intestinal environment of cesarean section infants birthed in the study region. SIGNIFICANCE: The intestinal microbiota and metabolites play important roles in infant development. To validate whether delivery modes influence the gut environment, we performed a detailed analysis of the earliest microbial colonization of the infant gut using a combination of 16S rRNA gene amplicon sequencing and LC-MS-based metabolomics. We found that the gut microbiota and metabolite composition were significantly different between vaginally delivered infants and cesarean section delivered infants. Our findings establish a vital baseline for studies tracking the infant gut microbiota and metabolite development following different delivery modes, and their associated effects on infant health. This study provides preliminary evidence that the observed differences due to delivery modes highlight their importance in shaping the early intestinal microbiota and metabolites.

13.
Med Sci Monit ; 26: e928573, 2020 Dec 29.
Artigo em Inglês | MEDLINE | ID: mdl-33373333

RESUMO

BACKGROUND Hyperammonemia has been reported in some critically ill patients with sepsis who do not have hepatic failure. A significant proportion of patients with non-hepatic hyperammonemia have underlying sepsis, but the association between non-hepatic hyperammonemia and prognosis is unclear. MATERIAL AND METHODS Information about patients with sepsis and non-hepatic hyperammonemia was retrieved from the Medical Information Mart for Intensive Care-III database. Survival rates were analyzed using the Kaplan-Meier method. Multivariate logistic regression models were employed to identify prognostic factors. Receiver operating characteristic (ROC) curve analysis was used to measure the predictive ability of ammonia in terms of patient mortality. RESULTS A total of 265 patients with sepsis were enrolled in this study. Compared with the non-hyperammonemia group, the patients with hyperammonemia had significantly higher rates of hospital (59.8% vs. 43.0%, P=0.007), 30-day (47.7% vs. 34.8%, P=0.036), 90-day (61.7% vs. 43.7%, P=0.004), and 1-year mortality (67.3% vs. 49.4%, P=0.004). In the survival analysis, hyperammonemia was associated with these outcomes. Serum ammonia level was an independent predictor of hospital mortality. The area under the ROC curve for the ammonia levels had poor discriminative capacity. The hyperammonemia group also had significantly lower Glasgow Coma Scale scores (P=0.020) and higher incidences of delirium (15.9% vs. 8.2%, P=0.034) and encephalopathy (37.4% vs. 19.6%, P=0.001). Intestinal infection and urinary tract infection with organisms such as Escherichia coli may be risk factors for hyperammonemia in patients who have sepsis. CONCLUSIONS Higher ammonia levels are associated with poorer prognosis in patients with sepsis. Ammonia also may be associated with sepsis-associated encephalopathy. Therefore, we recommend that serum ammonia levels be measured in patients who are suspected of having sepsis.

14.
Medicine (Baltimore) ; 99(45): e22859, 2020 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-33157928

RESUMO

BACKGROUND: This article will evaluate the effects of traditional Chinese medicine (TCM) combined with chemotherapy on the immune function and quality of life of patients with non-small cell lung cancer (NSCLC), and evaluate the published side effects. METHODS: The systematic review and meta-analysis will be conducted in accordance with the Preferred Reporting Items for Systematic Review and Meta-Analysis guidelines. The databases we will search include: PubMed, EMBASE, Cochrane Library, Web of Science, China National Knowledge Infrastructure, China Biomedicine, Wan fang Data, and Technology Periodical Database. The search date is from inception to June 30, 2020. There are no restrictions on the document language. The literatures included in this study are randomized controlled trials. The main results include ratio of CD3, CD4, CD8, CD4/CD8, NK cells, the level of IgA, IgG, IgM, and Karnofsky performance status score. The secondary result is to evaluate various side effects during treatment. We will use the Cochrane Collaboration tool to evaluate each study and use Review Manager software (RevMan, version 5.3) to merge and analyze the data. The 2 researchers will independently cross-screen the literature, extract data, and evaluate the quality. If there are differences, we will resolve them through discussion or consultation with a third reviewer. RESULTS: The results of this study will provide high-quality evidence for the effect of TCM combined with chemotherapy on the immune function and quality of life of patients with NSCLC. CONCLUSION: This article will comprehensively evaluate the effects of TCM combined with chemotherapy on the immune function and quality of life of patients with NSCLC, and provide evidence-based evidence for clinical practice. ETHICS: Since the data used in this study is based on previous trials and does not involve patient privacy, ethical approval is not required. STUDY REGISTRATION NUMBER: INPLASY202070071.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/terapia , Neoplasias Pulmonares/terapia , Medicina Tradicional Chinesa , Qualidade de Vida , Antineoplásicos/uso terapêutico , Complexo CD3/metabolismo , Linfócitos T CD4-Positivos/metabolismo , Linfócitos T CD8-Positivos/metabolismo , Carcinoma Pulmonar de Células não Pequenas/sangue , Humanos , Isotipos de Imunoglobulinas/sangue , Células Matadoras Naturais/metabolismo , Neoplasias Pulmonares/sangue , Metanálise como Assunto , Projetos de Pesquisa , Revisões Sistemáticas como Assunto
15.
Medicine (Baltimore) ; 99(45): e23151, 2020 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-33157998

RESUMO

During sepsis, platelets dysfunction contributes to organ dysfunction. Studies on platelets dysfunction in the long-term prognosis of sepsis are lacking. The aim of this study was to assess the role of platelets in the long-term prognosis of sepsis patients.A total of 4576 sepsis patients were extracted from MIMIC III Database. Survival was analyzed by the Kaplan-Meier method. Univariate and multivariate cox analyses were performed to identify prognostic factors. Significant prognostic factors were combined to build a nomogram to predict 1 year overall survival (OS). The discriminative ability and predictive accuracy of the nomogram were evaluated using the receiver operating characteristic curve (ROC) analysis and calibration curves used for sepsis.The more abnormal the platelet level, the worse prognosis of patients. After final regression analysis, age, blood urea nitrogen, platelets, international normalized ratio, partial thromboplastin time, potassium, hemoglobin, white blood cell count, organ failures were found to be independent predictors of 1 year OS of sepsis patient and were entered into a nomogram. The nomogram showed a robust discrimination, with an area under the receiver operating characteristic curve of 0.752. The calibration curves for the probability of the prognosis of sepsis patients showed optimal agreement between the probability as predicted by the nomogram and the actual probability.Platelet was an independent prognostic predictor of 1 year OS for patients with sepsis. Platelet-related nomogram that can predict the 1 year OS of sepsis patients. It revealed optimal discrimination and calibration, indicating that the nomogram may have clinical utility.


Assuntos
Plaquetas , Sepse/sangue , Sepse/mortalidade , Idoso , Estudos de Coortes , Bases de Dados Factuais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Nomogramas , Prognóstico , Estudos Retrospectivos , Taxa de Sobrevida , Fatores de Tempo
16.
Front Med (Lausanne) ; 7: 589825, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33195354

RESUMO

Background and Aims: Hyperammonemia usually develops because of hepatic disease, but it may occur in patients with non-hepatic hyperammonemia (NHH). But, studies on the prognosis and possible risk factors of this disorder are lacking. The aim of this study was to find possible prognostic and risk factors for NHH in critically ill patients. Methods: Data were extracted from MIMIC III Database. Survival was analyzed by the Kaplan-Meier method. Univariate and multivariate analyses were performed to identify prognostic factors. Results: Valproic acid, carbamazepine, corticosteroids, recent orthopedic surgery, epilepsy, disorders of urea cycle metabolism, and obesity were found to be risk factors for NHH. Patients in the hyperammonemia group had a higher 30 day mortality than those in the non-hyperammonemia group. After final regression analysis, ammonia was found to be independent predictors of mortality. Conclusion: Ammonia was an independent prognostic predictor of 30 day mortality for critical care patients without liver disease.

17.
Diabetes ; 2020 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-33199363

RESUMO

Pancreatic ß cell dysfunction plays a decisive role in progression of type 2 diabetes. Retinol binding protein 4 (RBP4) is a prominent adipokine in type 2 diabetes while its effect on ß cell function remains elusive and the underlying mechanisms are unknown. Here, we found that elevated circulating RBP4 levels were inversely correlated with pancreatic ß cell function in db/db mice across different glycemic stages. RBP4 directly suppressed glucose stimulated insulin secretion (GSIS) in primary isolated islets and INS-1E cells in a dose- and time-dependent manner. RBP4-transgenic overexpressing mice (RBP4-Tg) showed a dynamic decrease of GSIS which appeared as early as 8-week-old preceding the impairment of insulin sensitivity and glucose tolerance. Islets isolated from RBP4-Tg mice showed a significant decrease of GSIS. Mechanistically, we demonstrated that the stimulated by retinoic acid 6(STRA6), RBP4's only known specific membrane receptor, is expressed in ß cells and mediates the inhibitory effect of RBP4 on insulin synthesis via JAK2/STAT1/ISL-1 pathway. Moreover, decreasing circulating RBP4 level could effectively restore ß cell dysfunction and ameliorate hyperglycemia in db/db mice. These observations revealed a role of RBP4 in pancreatic ß cell dysfunction which provided new insight into the diabetogenic effect of RBP4.

18.
Artigo em Inglês | MEDLINE | ID: mdl-33208082

RESUMO

Sepsis-associated encephalopathy causes long-term health problems in patients with sepsis. This review ex-plores the pathogenesis of sepsis-associated encephalopathy, including its effects on the blood-brain barrier, microglia ac-tivation, mitochondrial dysfunction, the inflammatory medium and neurotransmitters and its roles in amino acid balance disorders, hyperammonemia, and intestinal flora imbalance. Understanding the etiology of sepsis-associated encephalopa-thy may allow the development of adjunctive therapies targeting its underlying mechanism and help develop preventative strategies.

19.
Small ; 16(47): e2005302, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33136347

RESUMO

Free-standing electrodes with high energy density and long life are of critical importance to the development of lithium-ion batteries (LIBs) for flexible/wearable electronic devices. Herein, the free-standing and foldable V2 O3 /multichannel carbon nanofibers (V2 O3 /MCCNFs) composites are prepared via electrospinning and subsequent carbonization. Such V2 O3 /MCCNFs electrode delivers a superior capacity of 881.1 mAh g-1 at 0.1 A g-1 after 240 cycles. More importantly, the ultralong lifespan is achieved with a high capacity of 487.8 mAh g-1 even after 5000 cycles at a high current density of 5 A g-1 with only 0.00323% decay rate, which shows the best performance among the reported V2 O3 -based anodes and other metal oxides based free-standing anodes. Furthermore, this flexible electrode is further applied to the pouch cell, which exhibits prominent capacity of 348.3 mAh g-1 after 500 cycles at 1 A g-1 with 0.094% decay per cycle. The unprecedented performance can be ascribed to synergetic contributions of V2 O3 and multichannel carbon nanofibers, which not only promote the penetration of electrolyte and reduce the transport length of Li+ , but also increase active material/electrolyte contact area and buffer the volume change. This work paves the way to develop free-standing electrode for flexible/wearable electronic devices with ultralong lifespan.

20.
Small ; : e2004925, 2020 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-33140582

RESUMO

Sodium-ion batteries (SIBs) are receiving considerable attention as economic candidates for large-scale energy storage applications. Na3 V2 (PO4 )2 O2 F (NVPF) is intensively regarded as one of the most promising cathode materials for SIBs, due to its high energy density, fast ionic conduction, and robust Na+ -super-ionic conductor (NASICON) framework. However, poor rate capability ascribed to the intrinsically low electronic conductivity severely hinders their practical applications. Here, high-rate and highly reversible Na+ storage in NVPF is realized by optimizing nanostructure and rational porosity construction. Hierarchical porous NVPF hollow nanospheres are designed to modify the issues of inconvenient electrolyte transportation and unfavorable charge transfer behavior faced by solid-structured electrode materials. The individual unique nanosphere is assembled from numerous nanoparticles, which shortens the length of Na+ transport in solid state and thus facilites the Na+ migration. Hollow nanostructure hierarchically porous configuration enables adequate electrolyte penetration, continuous electrolyte supplementation, and facile electrolyte transportation, leading to barrier-free Na+ /e- diffusion and high-rate cycling. In addition, the large electrolyte accessible surface area boosts the charge transfer in the whole electrode. Therefore, the present NVPF demonstrates unprecedented rate capability (85.4 mAh g-1 at 50 C) and long-term cyclability (62.2% capacity retention after 2000 cycles at 20 C).

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