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1.
Artigo em Inglês | MEDLINE | ID: mdl-32029582

RESUMO

Epigenetic alterations and metabolic dysfunction are two hallmarks of aging. However, the mechanism of how their interaction regulates aging, particularly in mammals, remains largely unknown. Here we show ELOVL fatty acid elongase 2 (Elovl2), a gene whose epigenetic alterations are most highly correlated with age prediction, contributes to aging by regulating lipid metabolism. Impaired Elovl2 function disturbs lipid synthesis with increased endoplasmic reticulum stress and mitochondrial dysfunction, leading to key accelerated aging phenotypes. Restoration of mitochondrial activity can rescue age-related macular degeneration (AMD) phenotypes induced by Elovl2 deficiency in human retinal pigmental epithelial (RPE) cells. We revealed an epigenetic-metabolism axis contributing to aging and potentially to antiaging therapy.

2.
Spectrochim Acta A Mol Biomol Spectrosc ; 231: 118096, 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-32006915

RESUMO

In this study, a simple low-temperature route is presented for the synthesis of Mg and Ce co-doped ZnO quantum dots (QDs). X-ray diffraction, transmission electron microscopy, electron paramagnetic resonance, X-ray photoelectron spectroscopy, UV-vis absorption spectra, and fluorescence measurements were used to characterize the synthesized QDs. The results indicate that the oxygen vacancy concentration could be tuned via Mg and Ce ions doping, which leads to the regulation of luminescence. The visible emission was directly associated with oxygen vacancies, and a blue shift of the visible emission with increasing Ce doping concentration was due to the quantum confinement effect. Finally, we explored the application of Mg and Ce co-doped ZnO QDs by fabricating a white LED device. Notably, the white LED device presents good luminescence properties under a voltage of 3 V and a driven current of 200 mA. The Commission International de l'Eclairage chromaticity, the correlated color temperature, and the color rendering index were determined to be (x = 0.32, y = 0.30), 5733 K, and 81, respectively, which make them potential candidates as single-phased QDs for white light-emitting diodes.

3.
Sci Total Environ ; 716: 137120, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32059321

RESUMO

LiAl hydrotalcite-like compound (LiAl HTlc) was synthesized via a hydrothermal method and used to adsorb Cu2+ and Zn2+ for investigating the adsorption characteristics of heavy metal cations. The X-Raydiffraction (XRD), fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and transmission electron microscopy (TEM) characterizations revealed the interconnecting flower-like layered structure of LiAl HTlc. The adsorption kinetics and isotherms of Cu2+ and Zn2+ on LiAl HTlc agreed with the pseudo-second-order model and the Langmuir model at a given sorbent concentration (Cs), respectively. The Cs-effect on the adsorption kinetics and isotherms was observed, and the Langmuir-surface component activity (SCA) equation could be utilized to characterize the effect of Cs in the adsorption isotherms. The adsorption process was spontaneous and endothermic. The adsorption mechanism denoted that the adsorption process was controlled using two main mechanisms, i.e., surface complexation and isomorphic substitution. This is the first report, to the best of our knowledge, on the usage of LiAl HTlc for the removal of heavy metal cations Cu2+ and Zn2+ from a solution. LiAl HTlc is a promising sorbent for treating water containing heavy metal cations.

4.
BMC Complement Med Ther ; 20(1): 7, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32020868

RESUMO

BACKGROUND: As the dry rhizome of Anemone raddeana Regel, Rhizoma Anemones Raddeanae (RAR), which belongs to Ranunculaceae, is usually used to treat wind and cold symptoms, hand-foot disease and spasms, joint pain and ulcer pain in China. It is well known that the efficacy of RAR can be distinctly enhanced by processing with vinegar due to the reduced toxicity and side effects. However, the entry of vinegar into liver channels can cause a series of problems. In this paper, the differences in the acute toxicity, anti-inflammatory and analgesic effects between RAR and vinegar-processed RAR were compared in detail. The changes in the chemical compositions between RAR and vinegar-processed RAR were investigated, and the mechanism of vinegar processing was also explored. METHODS: Acute toxicity experiments were used to examine the toxicity of vinegar-processed RAR. A series of studies, such as the writhing reaction, ear swelling experiment, complete Freund's adjuvant-induced rat foot swelling experiment and cotton granuloma, in experimental mice was conducted to observe the anti-inflammatory effect of vinegar-processed RAR. The inflammatory cytokines of model rats were determined by enzyme-linked immunosorbent assay (ELISA). Liquid Chromatography-Quadrupole-Time of Flight mass spectrometer Detector (LC-Q-TOF) was used to analyse the chemical compositions of the RARs before and after vinegar processing. RESULTS: Neither obvious changes in mice nor death phenomena were observed as the amount of vinegar-processed RAR in crude drug was set at 2.1 g/kg. Vinegar-processed RAR could significantly prolong the latency, reduce the writhing reaction time to reduce the severity of ear swelling and foot swelling, and remarkably inhibit the secretion of Interleukin-1ß(IL-1ß), Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) proinflammatory cytokines. The content of twelve saponins (e.g., Eleutheroside K) in RAR was decreased after vinegar processing, but six other types (e.g., RDA) were increased. CONCLUSIONS: These results revealed that vinegar processing could not only improve the analgesic and anti-inflammatory effects of RAR but also reduce its own toxicity. TRIAL REGISTRATION: Not applicable.

5.
Ecotoxicol Environ Saf ; 191: 110236, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-32001424

RESUMO

Copper (Cu), a transition metal, is an essential trace element in human and animal nutrition at low concentration, but Cu has toxic effects on tissues and organs at high concentration. Endoplasmic reticulum (ER) is a toxicological target in Cu poison. Thus far, no studies have focused on the relationship among copper, endoplasmic reticulum (ER) stress and apoptosis in animal and human livers. In the present study, mice treated with copper sulfate (CuSO4) were used to assess the impacts of copper on ER stress and hepatic apoptosis. A total of 240 mice were orally administered with 0 (control), 10, 20 and 40 mg/kg of CuSO4 for 42 days. The results indicated that CuSO4 at 10 mg/kg markedly induced hepatocyte apoptosis and ER stress. In addition, ER stress was characterized by the increased mRNA and protein levels of glucose-regulated protein 78 (GRP78) and 94 (GRP94). Furthermore, ER stress-triggered 3 apoptotic pathways were also activated by the increased intracellular calcium and up-regulated expression levels of genes involved in growth arrest- and DNA damage-inducible gene 153 (Gadd153/CHOP), c-Jun N-terminal kinase (JNK) and cysteine aspartate-specific protease 12 (caspase-12) signaling pathways in CuSO4-treated mice. In conclusion, CuSO4-induced ER stress can promote hepatic apoptosis in mice by activating CHOP, JNK and caspase-12 signaling pathways.

6.
Nanoscale ; 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32043508

RESUMO

Based on ab initio calculations and the Wannier-based tight-binding method, we studied the topological electronic properties and strain modulation of transition metal monochalcogenides (TMM) Mo2XY (X, Y = S, Se, Te, X ≠ Y). These materials are nodal line semimetals in the absence of spin-orbit coupling (SOC). The presence of SOC turns them into Weyl semimetals with 24 Weyl nodes located in the kz ≠ 0 planes and related by time-reversal, rotation C3z, and mirror symmetries. The maximal separation between two neighboring Weyl points with opposite chirality is of the order of magnitude of 0.10 Å-1, which can be readily accessed by angle-resolved photoemission spectroscopy (ARPES). The Weyl semimetal phase shows great robustness and demonstrates different responses under uniaxial and biaxial strain. Intriguingly, the location of the Weyl point changes significantly, resulting in a striking modulation of topological properties under in-plane biaxial strain. Our finding provides a realistic and promising platform for studying and manipulating the behavior of Weyl fermions in experiments.

7.
Chin J Integr Med ; 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31974753

RESUMO

BACKGROUND: Knee osteoarthritis (OA) is a major cause of disability among the older adults. Few treatments are safe and effective. Moxibustion is commonly used in treating knee OA in Chinese medicine (CM). CO2 Laser moxibustion device is a substitute for traditional moxibustion, which mimics the effects of traditional moxibustion. More data are needed to support its application in knee OA. OBJECTIVE: The trial aims to assess the effect and safety of CO2 laser moxibustion in patients with knee osteoarthritis compared with a sham control. METHODS: This is a protocol for a multicenter, randomized, double-blind, placebo-controlled trial. A total of 392 participants were recruited and assigned to the CO2 laser moxibustion group and sham laser moxibustion group with a 1:1 ratio at 6 outpatient clinics in Shanghai, China. Participants in both groups received treatment at the affected knee(s) at the acupuncture point Dubi (ST 35) and an Ashi point. There were 3 sessions per week for 4 weeks, and an additional 20-week follow-up. Primary outcomes were changes in the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) pain scores at week 4. Secondary outcomes were WOMAC function score, stiffness score and overall score, VAS pain, Short-Form heath survey (SF-36), and patients' global assessment. The serum levels of cytokines involved in progress of knee OA were explored. Safety was assessed during the whole trial. Masking effectiveness was assessed by both participants and treatment providers. DISCUSSION: CO2 laser moxibustion device, designed as a substitute for CM moxibustion, is easy to use and control with no choking smoke and smell, and is a plausible method for double-blind research. This study would provide rigorous evidence for the effect and safety of CO2 laser moxibustion in treating knee OA (Trial registration No.: ISRCTN15030019).

8.
BMJ Open ; 10(1): e034290, 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31911525

RESUMO

INTRODUCTION: Migraine is the second-leading cause of years lived with disability worldwide. The high prevalence of migraine-related emotional disorders is often overlooked. Acupuncture is often used to treat both migraine and emotional disorders. This systematic review protocol aims to analyse whether acupuncture is effective for treating emotional disorders in patients with migraine. METHODS AND ANALYSIS: Nine databases will be searched from inception to may 2019: cochrane central register of controlled trials, medline, embase, allied and complementary medicine database, cinahl, china national knowledge infrastructure, chinese biomedical literature database, vip database and wanfang database. Randomised controlled trials (rcts) of acupuncture therapy for migraine with emotional functioning outcomes, which were reported in chinese or english, will be included. The primary outcome is the change in emotional functioning. Study selection, data extraction and assessment of the risk of bias will be performed independently by two or more reviewers. Revman software (v.5.3) will be used to perform the assessment of the risk of bias and data synthesis. ETHICS AND DISSEMINATION: Ethics approval is not be needed because the data will not contain individual patient data, and there are no concerns about privacy. The results of this meta-analysis will be disseminated through publication in a peer-reviewed journal or relevant conference. TRIAL REGISTRATION NUMBER: CRD42019139433.

9.
Methods Mol Biol ; 2108: 29-42, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31939168

RESUMO

Interleukin (IL)-22 belongs to the IL-10 family of cytokines. IL-22 exerts its biological effects via members of the cytokine receptor family class 2. CD4+ T helper (Th) cells predominantly producing IL-22 have been designated as Th22 cells. IL-22/Th22 cells are functionally related to IL-17/Th17 cells, but are distinctly different. Both IL-22 and IL-17 are cytokines recruiting neutrophils in response to microbe invasion. In chronic inflammation, IL-22 mediates protective and regenerative processes, whereas IL-17 cytokines tend to induce inflammation. Studies found that increased IL-22 levels and Th22 cells in peripheral blood were associated with disease activity in patients with systemic lupus erythematosus (SLE), but decreased IL-22 and Th22 cells were also reported. Here we describe analysis of IL-22 and Th22 cells in peripheral blood quantified by flow cytometry, and correlate our findings with SLE disease activity.

10.
Water Res ; 172: 115494, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31954934

RESUMO

Microbial degradation is an elimination method for removal of organic contaminants from soil and water. However, the main factor limiting its practical application is high bacterial sensitivity to environmental factors such as pH, toxicity, and mass transfer. In this study, biochar was produced pyrolytically from peanut shells at 350 °C, 550 °C, and 750 °C (referred to as BC350, BC550, and BC750, respectively) and their promotion on phenol biodegradation in wastewater by the bacterium Pseudomonas citronellolis was investigated. Higher initial phenol concentration (>400 mg L-1) showed obvious inhibition on biodegradation with the removal efficiencies being less than 46%, and even the bacterium failed to survive at the phenol concentrations of higher than 1000 mg L-1. With biochar incorporated, the removal efficiencies of phenol increased from below 46% to up to 99% at the initial concentrations of 400-1200 mg L-1. Immobilization of strains in biochar by calcium alginate further increased the microbial tolerance to high concentrations of phenol (i.e., 63% removal at 1200 mg L-1). Scanning electron microscopy demonstrated that biochar acted as shelter to support the bacterium to struggle with extreme conditions. The initial adsorption of phenol by biochar alleviated the initial toxicity of phenol to bacterium and the subsequent gradual desorption controlled the bioavailability of phenol. In this regard, BC350 showed a comparable sorption capacity with BC550 and BC750, while a higher desorption potential than them, thus balanced better the toxicity and bioavailability of phenol to microbes. Alkalinity of BC550 and BC750 played important roles in rescuing the microbes from being damaged by pH shock via neutralizing the fast generation of acidic intermediates. The extractable organic substances in BC350 could be consumed by bacterium as substrates, which was confirmed by incubating the strains in water-extractable solution. Results of this study indicate that incorporation of microbes with biochar could promote the biodegradation of high concentration organic wastewater.

11.
Medicine (Baltimore) ; 99(1): e18558, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31895798

RESUMO

BACKGROUND: The invasive surgical intervention for neurogenic bladder dysfunction (NBD) following spinal cord injury (SCI) involves permanently altering one's body system and carries many surgical related risks and medication side effects are often seen in long term usage of pharmaceutical medications. Therefore, acupuncture and moxibustion therapies have been recommended due to their efficacy, simplicity of operation, cost effectiveness and safety. This protocol is designed for systematic review and network meta-analysis, which will perform comparisons or rankings of efficacy among the currently available acupuncture and moxibustion techniques and provide evidence to guide the best practice in acupuncture and moxibustion treatments of NBD due to SCI. METHODS/DESIGN: The Cochrane Library, EMBASE, PubMed, Web of Science, CENTRAL, CNKI, The VIP Database, The Wanfang database, CDFD, CMFD will be searched from inception to November 1, 2019. All randomized controlled trials containing eligible interventions(s) and outcome(s) will be included. The quality of included trials will be assessed using the "Risk of bias" tool from the Cochrane Handbook (V.5.1.0). Data analysis will be conducted by using STATA software (Version 13.0). Continuous outcome will be indicated as mean difference (MD) or standard mean difference (SMD), and enumeration data will be presented with odds risk (OR) or relative risk (RR). RESULTS: This systematic review and network meta-analysis study aims to determine the most effective and safe approach in relieving urinary symptoms, and whether it produces better results in urodynamic examination. And a high-quality ranking of the therapeutic classes will be presented. The report will follow the PRISMA checklist for network meta-analysis. Results of the search strategy and the study selection will be presented in a PRISMA compliant flow chart. CONCLUSION: This study aims to propose a standard clinical decision-making guideline for acupuncture and moxibustion treatment of NBD after SCI.


Assuntos
Terapia por Acupuntura/métodos , Moxibustão/métodos , Traumatismos da Medula Espinal/complicações , Bexiga Urinaria Neurogênica/terapia , Feminino , Humanos , Masculino , Meta-Análise em Rede , Ensaios Clínicos Controlados Aleatórios como Assunto , Projetos de Pesquisa , Revisão Sistemática como Assunto , Resultado do Tratamento , Bexiga Urinaria Neurogênica/etiologia
12.
Chin J Integr Med ; 26(1): 33-38, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31776963

RESUMO

OBJECTIVE: To investigate the effect of compound laser acupuncture-moxibustion on blood glucose, fasting insulin and blood lipids levels in type 2 diabetes mellitus (T2DM) rats. METHODS: Forty male Wistar rats were randomly divided into 4 groups, including the normal group, model control group, laser group and sham laser group (n=10 per group). The rats in the normal group were fed with a standard diet. Rats in other groups were fed with a high-sugar and high-fat diet for 4 weeks, then intraperitoneally injected with 1% streptozotocin to induce T2DM model. The laser group was irradiated by 10.6 µm and 650 nm compound laser on bilateral Pishu (BL 20), Shenshu (BL 23) and Sanyinjiao (SP 6) for 5 min, 6 times a week for 5 weeks. The sham laser group received the same treatment as the laser group, but without laser output. The model control group and normal group were not treated. Blood glucose levels were measured before and after 1, 2, 3, 4 and 5 weeks of treatment. The serum levels of fasting insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) were analyzed after the last treatment. RESULTS: The blood glucose levels in the model control group increased during the 5 weeks of treatment compared with the normal group (P<0.05), while those in the laser group were significantly lower than the model control group after weekly treatment (P<0.01 or P<0.05). After 1, 2 and 3 weeks of treatment, the blood glucose levels in the laser group decreased obviously compared with the sham laser group (P<0.01 or P<0.05). Compared with the normal group, the levels of fasting insulin, TC and LDL in the model control group notably increased (P<0.01 or P<0.05), while their levels in the laser group were significantly lower than the model control group after 5 weeks of treatment (P<0.05 or P<0.01). However, no statistically significant differences were observed in TG or HDL levels among the 4 groups (P>0.05). CONCLUSION: The compound laser acupuncture-moxibustion of 10.6 µm and 650 nm had positive effects on the regulation of hyperglycemia and insulin resistance in T2DM rats, which may be a potential treatment for T2DM, and also provide an alternative to the traditional acupuncture and moxibustion therapy.

13.
J Clin Invest ; 130(1): 438-450, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31815740

RESUMO

An excess of fecal bile acids (BAs) is thought to be one of the mechanisms for diarrhea-predominant irritable bowel syndrome (IBS-D). However, the factors causing excessive BA excretion remain incompletely studied. Given the importance of gut microbiota in BA metabolism, we hypothesized that gut dysbiosis might contribute to excessive BA excretion in IBS-D. By performing BA-related metabolic and metagenomic analyses in 290 IBS-D patients and 89 healthy volunteers, we found that 24.5% of IBS-D patients exhibited excessive excretion of total BAs and alteration of BA-transforming bacteria in feces. Notably, the increase in Clostridia bacteria (e.g., C. scindens) was positively associated with the levels of fecal BAs and serum 7α-hydroxy-4-cholesten-3-one (C4), but negatively correlated with serum fibroblast growth factor 19 (FGF19) concentration. Furthermore, colonization with Clostridia-rich IBS-D fecal microbiota or C. scindens individually enhanced serum C4 and hepatic conjugated BAs but reduced ileal FGF19 expression in mice. Inhibition of Clostridium species with vancomycin yielded opposite results. Clostridia-derived BAs suppressed the intestinal FGF19 expression in vitro and in vivo. In conclusion, this study demonstrates that the Clostridia-rich microbiota contributes to excessive BA excretion in IBS-D patients, which provides a mechanistic hypothesis with testable clinical implications.

14.
J Hazard Mater ; 384: 121455, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31668763

RESUMO

Biochar as a soil amendment could be involved in redox process of elements which would be affected by soil-redox-active components including minerals and organic acids. This study evaluated the effects of Fe mineral and lactate on reducing capacity of biochar for Cr(VI) reduction and the underlying mechanisms. Fe minerals inhibited the reduction of Cr(VI) by biochar, with the decrease of Cr(VI) reduction rate constant obtained by pseudo first-order reaction model from 2.18 to 2.47 × 10-2 h-1 to 0.71-1.51 × 10-2 h-1. The decrease of reduction rate constant was because (1) the loss of electron donating moieties in biochar; and (2) inhibition of electron transfer between biochar and Cr(VI) due to surface coverage by biochar-Fe complex. However, the coexistence of Fe minerals with lactate enhanced the reduction of Cr(VI) by biochar, with the rate constant increasing from 2.58 to 3.05 × 10-2 h-1 to 2.91-27.2 × 10-2 h-1. The positive effect was also attributed to two reasons: (1) lactate can decrease the surface Fe-coverage of biochar through chelating process; (2) electron from lactate can be shuttled by Fe(II) and thus enhancing the Cr(VI) reduction. Our results revealed that different soil redox-active components could have varying effects on biochar amendment for Cr(VI) reduction, which should be further considered during the application of biochar.

15.
J Hazard Mater ; 382: 121033, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31561196

RESUMO

The pyrolytic production of Fe-enriched composite biochar is receiving increasing attention. However, understanding of the environmental risk from the polycyclic aromatic hydrocarbons (PAHs) potentially generated during composite biochar production is lacking. This study investigated the formation of PAHs from the pyrolysis of barley straw impregnated with FeCl3 or Fe(NO3)3 at 350 °C, 500 °C, and 650 °C. The total amount of PAHs formation increased with increasing heating temperature. Most of the PAHs were concentrated in bio-oil (72.7-94.6%), with only a small fraction retained in biochar (1.7-11.1%) and in biogas (2.2-16.2%). Preloading FeCl3 or Fe(NO3)3 onto the biomass greatly reduced PAH formation by up to 33% and 21%, respectively, compared to that obtained with biomass alone. The suppressed formation of PAHs was due to the generation of more reductive forms of Fe, such as Fe0 and FeO, in the O2-starved pyrolysis atmosphere, which reduced C2H2 and C6H5OH, two important PAH precursors in hydrogen abstraction acetylene addition reactions. Although Fe loading reduced the amounts of PAHs in biochar, the toxic equivalent value increased because Fe induced more accumulation of high-molecular-weight PAHs in the biochar. This study proved that Fe loading suppresses PAH generation during biomass pyrolysis, which can guide the design of composite biochar production.

16.
J Hazard Mater ; 386: 121973, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31884366

RESUMO

The aggregation kinetics and colloidal stability of CeO2-NPs in the presence of monovalent or divalent electrolytes, as well as inorganic (kaolin and goethite) and organic (humic acid, HA) colloids were evaluated using time-resolved dynamic light scattering, advanced spectroscopic tools, and theoretical calculations. Critical coagulation concentrations for CeO2-NPs were generally lower in CaCl2 than that in NaCl electrolyte. The negatively charged kaolin accelerated CeO2-NPs aggregation due to electrostatic attraction, whilst opposite phenomenon was observed for the positively charged goethite in NaCl solution. In CaCl2 solution, goethite destabilized CeO2-NPs because of its well-crystal structure and specific adsorption of Ca2+. The presence of 0.1 mg C/L HA decreased the surface charge of CeO2-NPs, resulting in lower critical coagulation concentrations. Increasing the HA concentration from 0.1 to 1 mg C/L improved CeO2-NPs stability, mainly via electrostatic and steric repulsion. The Ca2+-bridging and complexation contributed significantly to CeO2-NPs aggregation. Additional aggregation experiments in seven natural waters revealed that CeO2-NPs remained stable in water types with high contents of organic colloids and low levels of salts, thus having higher transport potential. These findings provided new insights into the interactive influence of naturally occurring colloids and ions on the heteroaggregation behavior and fate of CeO2-NPs.

17.
Aging (Albany NY) ; 11(23): 11659-11672, 2019 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-31822637

RESUMO

Nickel (Ni), an environmental hazard, widely causes allergic contact hypersensitivity worldwide. Despite that Ni-stimulated pro-inflammatory response is vital in allergy, the underlying molecular mechanisms remain largely unclear. Here, we demonstrated that NiCl2 activated nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPKs) and interferon regulatory factor 3 (IRF3) signaling pathways in primary bone marrow-derived macrophages (BMDMs), leading to the altered transcription levels of interleukin-1ß (IL-1ß), -6, -8, -18, tumor necrosis factor-α (TNF-α) and interferon ß (INF-ß). We also found that nickel chloride (NiCl2) activated Nod-like receptor 3 (NLRP3) inflammasome pathway, resulting in the proteolytic cleavage and release of IL-1ß. NiCl2 induced the accumulation of mitochondrial reactive oxygen species (mtROS) and the release of mitochondrial DNA (mtDNA), thus activating NLRP3 inflammasome pathway. Additionally, NiCl2-induced apoptosis was dependent on the generation of mtROS, and caspase-1 activation might also partly contribute to the apoptotic process. Altogether, abovementioned results indicate that NiCl2 induces inflammatory activation in BMDMs via NF-κB, MAPKs, IRF3 signaling pathways as well as NLRP3 inflammasome pathway, which provides a mechanism to improve the efficiency of treatment against Ni-induced allergic reactions.

18.
Aging (Albany NY) ; 11(24): 12476-12496, 2019 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-31825894

RESUMO

Understanding the bone and musculoskeletal system is essential to maintain the health and quality of life of our aging society. Mesenchymal stem cells (MSCs) can undergo self-renewal and differentiate into multiple tissue types including bone. We demonstrated that BMP9 is the most potent osteogenic factors although molecular mechanism underlying BMP9 action is not fully understood. Long noncoding RNAs (lncRNAs) play important regulatory roles in many physiological and/or pathologic processes. Here, we investigated the role of lncRNA Rmst in BMP9-induced osteogenic differentiation of MSCs. We found that Rmst was induced by BMP9 through Smad signaling in MSCs. Rmst knockdown diminished BMP9-induced osteogenic, chondrogenic and adipogenic differentiation in vitro, and attenuated BMP9-induced ectopic bone formation. Silencing Rmst decreased the expression of Notch receptors and ligands. Bioinformatic analysis predicted Rmst could directly bind to eight Notch-targeting miRNAs, six of which were downregulated by BMP9. Silencing Rmst restored the expression of four microRNAs (miRNAs). Furthermore, an activating Notch mutant NICD1 effectively rescued the decreased ALP activity caused by Rmst silencing. Collectively, our results strongly suggest that the Rmst-miRNA-Notch regulatory axis may play an important role in mediating BMP9-induced osteogenic differentiation of MSCs.

19.
Animals (Basel) ; 9(12)2019 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-31795481

RESUMO

This study determined the effects of increased consumption of sulfur amino acids (SAA), as either DL-Met or Hydroxy-Met (OH-Met), by sows and piglets on their performance and the ability of the progeny to resist a lipopolysaccharide (LPS) challenge. Thirty primiparous sows were fed a diet adequate in SAA (CON) or CON + 25% SAA, either as DL-Met or OH-Met from gestation day 85 to postnatal day 21. At 35 d old, 20 male piglets from each treatment were selected and divided into 2 groups (n = 10/treatment) for a 3 × 2 factorial design [diets (CON, DL-Met or OH-Met) and challenge (saline or LPS)]. OH-Met and/or DL-Met supplementation increased (p ≤ 0.05) piglets' body weight gain during day 0-7 and day 7-14. Sow's milk quality was improved in the supplemented treatments compared to the CON. The LPS challenge decreased (p ≤ 0.05) piglets' performance from 35 to 63 d and increased (p ≤ 0.05) the levels of aspartate aminotransferase, total bilirubin, IL-1ß, IL-6, TNF-a, and malondialdehyde. Plasma albumin, total protein, total antioxidant capacity and glutathione peroxidase decreased post-challenge. The results were better with OH-Met than DL-Met. The increase of Met consumption, particularly as OH-Met increased piglets' growth performance during the lactation phase and the challenging period.

20.
ACS Omega ; 4(23): 20275-20284, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31815230

RESUMO

Polyphosphazene microspheres were fabricated by ultrasonic-assisted precipitation polymerization using 4,4'-(hexafluoroisopropylidene)diphenol, 4,4'-sulfonyldiphenol, 4,4-(9-fluorenylidene)diphenol, and phenolphthalein to obtain poly[4,4'-(hexafluoroisopropylidene)diphenol]phosphazene (PZAF), poly(4,4'- sulfonyldiphenol)phosphazene (PZS), poly[4,4'-(9-fluorenylidene)diphenol]phosphazene, and poly(phenolphthalein)phosphazene (PZPT) and were incorporated into polybenzoxazines (PBa) to obtain corresponding PZAF/PBa, PZS/PBa, fluorenyl polyphosphazene (PZFP)/PBa, and PZPT/PBa composites. Addition of 5 wt % of PZAF, PZS, PZFP, and PZPT microspheres improved the thermal stability and fire retardancy of PBa resin significantly. Notably, addition of PBa with 5% PZAF led to a 62.5% decrease in the peak heat release rate and 49.3% reduction in total heat release. The role of microspheres in the gas-phase flame-retardancy mechanism in the PBa matrix was studied. Dynamic mechanical analysis results demonstrated that the T g of PBa flame-retardant composites was still around 210 °C compared to 221 °C of pure PBa. Hence, the synthesized PBa composites had potential applications as high flame-retardancy materials.

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