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1.
Food Chem ; 305: 125429, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31505415

RESUMO

A simple and rapid magnetic solid-phase extraction (MSPE) method using PEGylated multi-walled carbon nanotubes magnetic nanoparticles (PEG-MWCNTs-MNP) as absorbents is proposed for isolation and enrichment of aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), aflatoxin M1 (AFM1), aflatoxin M2 (AFM2), ochratoxin A (OTA), zearalenone (ZEA), zearalanone (ZAN), α-zeralanol (α-ZAL), ß-zeralanol (ß-ZAL), α-zeralenol (α-ZOL), and ß-zeralenol (ß-ZOL) from liquid milk. Combined with ultra-high performance liquid chromatography Q-Exactive high resolution mass spectrometry, simultaneous qualification of these mycotoxins was achieved with sensitivity and specificity. The proposed method showed a good linearity (R2 ≥ 0.995), high sensitivity (limit of detection in the range of 0.005-0.050 µg/kg and limit of quantification in the range of 0.015-0.150 µg/kg), adequate recovery (81.8-106.4%), and good repeatability (intra-day precision in the range of 2.1-8.5% and inter-day precision in the range of 3.9-11.7%). It has been successfully applied to the determination of 13 mycotoxins in real liquid milk samples.

2.
J Affect Disord ; 260: 84-90, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31493644

RESUMO

BACKGROUND: Previous studies have investigated the role of cognitive factors in the relationship between stressful life events and depression; however, few studies comprehensively considered cognitive and personality factors. Therefore, this study investigated the multiple mediating roles of fatalism and core self-evaluations in the relationship between stressful life events and depression. METHODS: A cross-sectional survey was conducted with 537 Chinese university students (Mage = 20.20, SD = 1.38) at two universities in Guizhou and Sichuan provinces. The independent variable was stressful life events; mediating variables were fatalism and core self-evaluations; and the dependent variable was extent of depression. Multiple mediation analysis was performed using the PROCESS macro in SPSS. RESULTS: Significant positive correlations were found among stressful life events, fatalism, and depression, while core self-evaluations were significantly negatively correlated with stressful life events, fatalism, and depression. After adjusting for demographic variables, stressful life events directly and positively influenced depression (ß = 0.370, 95% CI = 0.292-0.448). Fatalism and core self-evaluations played multiple mediating roles in the relationship between stressful life events and depression, with stressful life events influencing depression through three mediation pathways (total mediation effect = 0.199, 95% CI = 0.145-0.254), which accounted for 53.85% of the total effect. LIMITATIONS: The data used in this study were self-reported by university students and measureed via cross-sectional designs. CONCLUSIONS: Stressful life events can influence depression either directly or indirectly by simultaneously increasing fatalism and lowering core self-evaluations (parallel mediation) or decreasing core self-evaluations through increasing the level of fatalism (serial mediation).

3.
Anticancer Drugs ; 31(1): 60-66, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31609768

RESUMO

Leucine aminopeptidase 3 is involved in the progression and metastasis of several cancers. This study aimed to screen anti-tumor lead compounds targeting leucine aminopeptidase 3. The compounds' suppression effect on enzyme activity and anti-tumor activity were evaluated through a series of assays. Leucine aminopeptidase 3 overexpression K562 cells were used as an enzyme source to screen 43 natural marine compounds. Compounds 5 and 6 exhibited high suppression effect on leucine aminopeptidase 3 activity. Cell activity tests indicated that both compounds have an anti-proliferative effect on triple-negative breast cancer cells. Wound healing assay and transwell invasion assay showed that both compounds could inhibit the migration and invasion of breast cancer cells. Immunoblot analysis exhibited that both compounds could downregulate the expression of metastasis-related proteins fascin and matrix metalloproteinase-2/9. A molecular dynamic simulation process was applied to discover the key features of compounds 5 and 6 in binding to leucine aminopeptidase 3 active site. This study described the anti-tumor effects of two leucine aminopeptidase 3 small molecule inhibitors. Taken together, compounds 5 and 6 could be used as anti-tumor lead compounds targeting leucine aminopeptidase 3.

4.
Am J Otolaryngol ; 41(1): 102318, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31732299

RESUMO

PURPOSE: Abnormal DNA methylation plays an important role in clinical diagnosis and prognosis of various tumors. DNA methylation is catalyzed by DNA methyltransferase (DNMT). However, the methylation status of MAGE-A1 and MAGE-A3 promoter regions in LSCC is unclear. To investigate the methylation levels of MAGE-A1, -A3 in LSCC and corresponding normal tissues. The expression of DNMTs (DNMT1, DNMT3a and DNMT3b) in LSCC and the relationship between the methylation status of MAGE-A1, -A3 were analyzed. MATERIALS AND METHODS: We examined methylation status of MAGE-A1, -A3 in LSCC by using MSP. Meanwhile, the expression level of DNMTs in LSCC was detected by immunohistochemistry. And further analysis the correlation between DNMTs expression level and methylation status of MAGE-A1 and MAGE-A3. RESULTS: The unmethylation rate of MAGE-A1, -A3 were 39.62% and 46.23%. The expression of DNMTs was 33.02% to 37.74%. The level of demethylation of MAGE-A1 and MAGE-A3 were negative related to DNMTs protein. MAGE-A1 and MAGE-A3 unmethylation status and DNMT3a expression were independent prognostic factors for LSCC. CONCLUSIONS: The DNMTs may participate in the methylation process of MAGE-A1 and MAGE-A3, which may play an important role in the occurrence and development of LSCC.

5.
J Nanosci Nanotechnol ; 20(2): 1098-1108, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31383110

RESUMO

In this study, the 2D porous graphitic carbon nitride (g-C3N4) nanosheets were successfully fabricated via a facile thermal decomposition polymerization method without any help of templates, and then novel porous g-C3N4/CdS complex catalysts of different mass fractions were is situ synthesized by a simple solvothermal process. The results of photocatalytic experiments demonstrate that the coupling g-C3N4/CdS cocatalysts exhibit significant enhanced visible-light-driven photocatalytic activity for the decolorization of methyl orange (MO) compared with individual porous g-C3N4 and CdS. In particular, an optimal porous g-C3N4 content in the hybridized composite has been determined to be 70 wt.%, corresponding to pseudo-first-order rate constant of 0.046 min-1, which is 7 and 11 times faster than that of pure porous g-C3N4 and CdS, respectively. Photoluminescence (PL) spectroscopy measurements clearly confirmed that the recombination of photoproduced electrons and holes in g-C3N4/CdS composites was efficiently inhibited due to the formation of heterojunctions. Furthermore, the possible mechanism of enhanced photocatalytic activity and photostability of prous g-C3N4/CdS are also tentatively proposed.

6.
Carbohydr Polym ; 227: 115362, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31590882

RESUMO

A new polysaccharide (pFSP) was first isolated from the originally discarded fibrous roots part of Chinese traditional herb, Bletilla striata. pFSP was composed of D-glucose, D-galactose and D-mannose in a molar ratio of 1: 2.03: 3.45 with molecular weight of 9.1 × 104 Da. It could effectively scavenge DPPH and superoxide radicals with inhibition rate of 64.47% and 72.27% at 5.0 mg/ml, higher than that of polysaccharide from Bletilla striata tuber. Structural investigations of the periodate oxidation studies and Smith-degradation as well as the FT-IR spectroscopy were performed, and combined with 1D and 2D NMR spectroscopy, the repeating unit of pFSP contained (1→4)-linked-α-D-Glcp, (1→4)-linked-ß-D-Manp and (1→3,6)-linked-ß-D-Manp units, together with the branches of (1→6)-linked-ß-D-Galp and terminated with (1→)-linked-ß-D-Manp residue.

7.
Nanotechnology ; 31(2): 025403, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31550690

RESUMO

Lithium-sulfur (Li/S) batteries are promising portable energy storage devices if the defects of insufficient conductivity and obvious shuttle effect can be effectively suppressed. In this work, a unique caddice-like ball carbon nanotubes/CoO (CNTs/CoO) microspheres have been synthesized through a facile spray drying and following calcination method. By using cobalt acetate (Co(CHCOO)2) as a cobalt source, polymethyl methacrylate (PMMA) as a pore former and CNTs as the supporting frame, the as-prepared CNTs/CoO microspheres endowed with enriched interconnected pores and abundant scattered CoO nanoparticles on CNT walls. CNTs provided physical adsorption platforms for adsorption of polysulfides while ensuring the conductivity of the overall material. Polycrystalline CoO nanoparticles are uniformly deposited on CNT walls, providing additional confinement of polysulfides by strong chemical adsorption. In addition, the different content of CoO in the microspheres was regulated by the amount of added cobalt source during the preparation process. With both physical entrapment by CNTs and strong chemical interaction with CoO nanocrystals, this unique design can effectively promote the active material utilization and inhibit the shuttle effect. When employed as the sulfur host, an excellent electrochemical performance was achieved on the resulting sulfur-impregnated CNTs/CoO (S-CNTs/CoO) composite with the sulfur content of 73.0%. The obtained S-CNTs/CoO microspheres delivered an exceptional initial discharge capacity of 1340 mAh g-1 and a prominent cycling stability of 1116 mAh g-1 after 100 cycles at 0.2 C, as well as a superb rate capability of 600 mAh g-1 and 506 mAh g-1 at 2.0 C and 3.0 C, respectively. The results revealed that the S-CNTs/CoO composite was an enviable cathode material and showed an excellent potentiality in the application of Li/S batteries.

8.
Eur J Protistol ; 72: 125646, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31785489

RESUMO

A new urostylid ciliate, Anteholosticha songi nov. spec., isolated from forest soil in Tibet, and an American population of Holosticha pullaster (Müller, 1773) Foissner et al., 1991, isolated from a freshwater pond in the USA, are investigated in terms of their morphology, ontogenesis, and molecular biology. Anteholosticha songi nov. spec. is characterized by a slender to ellipsoidal body measuring 160-205 × 40-55 µm in vivo; rod-shaped yellowish cortical granules arranged in irregular short rows; four dorsal kineties; adoral zone consisting of 35-40 membranelles; three frontal, one buccal, one parabuccal, two frontoterminal, two pretransverse, and four to six transverse cirri and 14-25 midventral pairs; 12-22 ellipsoidal macronuclear nodules longitudinally arranged in pairs left of cell mid-line. Supplemental information on morphogenesis in Holosticha pullaster is also presented. The phylogenetic relationship of Anteholosticha and Holosticha inferred from SSU rDNA sequence data are concordant with previous studies and showing that Holosticha is monophyletic whereas Anteholosticha is polyphyletic and should be split into two or more genera.

9.
Psych J ; 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31788984

RESUMO

Impairment in semantic association has been reported in bipolar disorder (BD) and schizophrenia (SZ) patients and could underlie abnormal speech patterns in both disorders. In this study, we compared the electrophysiological semantic processing features in patients with these two disorders. Participants (n = 61; BD = 19; SZ = 19; healthy controls [HCs] = 23) were administered a semantic judgment task and event-related potentials (ERPs) were recorded. Responses of the two patient groups were significantly slower than HCs, but comparable behavioral semantic priming effects were observed in both patient groups. The N400 priming effect was observed in all groups, with a delayed peak in the two patient groups. The N400 effect was enhanced for both BD and SZ patients over the left frontal and frontal pole region, but SZ patients showed additional reduction of N400 over the right posterior and occipital regions. The N400 mean amplitudes for related targets correlated with less severe negative symptoms in patients with SZ. Discriminant functional analysis using reaction time and N400 measures successfully classified 82% of the participants into their respective clinical groups. These results suggest that patients with BD and SZ have both overlapping and distinctive semantic processing dysfunction. These findings are consistent with the continuum conceptualization of these disorders, but also offer some support for the traditional Kraepelinian dichotomy.

10.
Neuroreport ; 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31789691

RESUMO

The present study examined the roles of 5-HT2A, 5-HT2B and 5-HT2C receptor subtypes in mediating the ventrolateral orbital cortex (VLO)-induced antiallodynia in a rat model of neuropathic pain induced by spared nerve injury (SNI). Change of mechanical paw withdrawal threshold (PWT) was measured using von-Frey filaments. Microinjection of preferential or selective 5-HT2A/C, 5-HT2B and 5-HT2C receptor agonists, (±)-1-(2,5-Dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride (DOI), α-methyl-5-(2-thienylmethoxy)-1H-Indole-3-ethanamine hydrochloride (BW723C86) and 1-(3-Chlorophenyl)-piperazine hydrochloride (m-CPP) into the VLO significantly depressed allodynia induced by SNI, and the inhibitory effect of DOI was blocked or attenuated by selective 5-HT2A/C receptor antagonists ketanserin (+)-tartrate salt (ketanserin) and 5-HT2A receptor antagonist R-(+)-alpha-(2,3-dimethoxyphenyl)-1-[2-(4-fluorophenylethyl)]-4-piperidinemethanol (M100907); the effects of BW723C86 and m-CPP were antagonized by 5-HT2B receptor antagonists N-(1-Methyl-1H-5-indolyl)-N'-(3-methyl-5-isothiazolyl)urea (SB204741) and 5-HT2C receptor antagonist RS102221 hydrochloride hydrate (RS-102221), respectively. These results suggest that 5-HT2A, 5-HT2B, 5-HT2C receptor subtypes are involved in mediating the VLO-induced antiallodynia in the neuropathic pain state.

11.
Future Oncol ; 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31793364

RESUMO

Aim: Patient-Generated Subjective Global Assessment (PG-SGA) and Nutritional Risk Screening 2002 (NRS2002) are used to evaluate patients' nutritional status. Materials & methods: The data of 114 gastric cancer patients with pyloric obstruction treated between July 2016 and July 2017 were assessed retrospectively. Results: Based on clinical evaluation, 70.1% had malnutrition, with 61.4% at nutritional risk by NRS2002 and 66.7% by PG-SGA. The area under the receiver operating characteristic curve was 0.858 for PG-SGA and 0.706 for NRS2002. Sensitivity and specificity were 89 and 85% for PG-SGA and 78 and 76% for NRS2002. In both assessments, patients at risk showed more postoperative complications. Conclusion: PG-SGA was more suitable for evaluating the preoperative nutritional status of gastric cancer patients with pyloric obstruction, with higher diagnostic efficacy.

12.
Cell Death Differ ; 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31802034

RESUMO

Infiltrated macrophages are an important constituent of the tumor microenvironment and play roles in tumor initiation and progression by promoting immune evasion. However, the molecular mechanism by which macrophage-derived cytokines foster immune escape of colorectal cancer (CRC) is unclear. Here, we demonstrated that macrophage infiltration induced by lipopolysaccharide (LPS) or a high-cholesterol diet (HCD) significantly promoted CRC growth. Similarly, LPS and poly (I:C) remarkably increased the volume of CT26 cell allograft tumors. C-C motif chemokine ligand 5 (CCL5), which is secreted by macrophages, inhibited T-cell-mediated killing of HT29 cells and promoted immune escape by stabilizing PD-L1 in vitro and in vivo. Mechanistically, CCL5 resulted in formation of nuclear factor kappa-B p65/STAT3 complexes, which bound to the COP9 signalosome 5 (CSN5) promoter, leading to its upregulation. Moreover, CSN5 modulated the deubiquitination and stability of PD-L1. High expression of CSN5 in CRC was associated with significantly shorter survival. Furthermore, compound-15 was identified as an inhibitor of CSN5, and destabilized PD-L1 to alleviate the tumor burden. Our results suggest that the novel CCL5-p65/STAT3-CSN5-PD-L1 signaling axis is significantly activated by LPS or HCD-driven macrophage infiltration in an animal model of CRC, which likely has therapeutic and prognostic implications for human cancers.

13.
Chin J Integr Med ; 25(12): 902-910, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31802424

RESUMO

OBJECTIVE: To investigate the potential efficacy of panaxadiol saponins component (PDS-C) in the treatment of aplastic anemia (AA) model mice. METHODS: Totally 70 mice were divided into 7 groups as follows: normal, model, low-, medium-, high-dose PDS-C (20, 40, 80 mg/kg, namely L-, M-, H-PDS-C), cyclosporine (40 mg/kg), and andriol (25 mg/kg) groups, respectively. An immune-mediated AA mouse model was established in BALB/c mice by exposing to 5.0 Gy total body irradiation at 1.0 Gy/min, and injecting with lymphocytes from DBA mice. On day 4 after establishment of AA model, all drugs were intragastrically administered daily for 15 days, respectively, while the mice in the normal and model groups were administered with saline solution. After treatment, the peripheral blood counts, bone marrow pathological examination, colony forming assay of bone marrow culture, T lymphocyte subpopulation analysis, as well as T-bet, GATA-3 and FoxP3 proteins were detected by flow cytometry and Western blot. RESULTS: The peripheral blood of white blood cell (WBC), platelet, neutrophil counts and hemoglobin (Hb) concentration were significantly decreased in the model group compared with the normal group (all P<0.01). In response to 3 dose PDS-C treatment, the WBC, platelet, neutrophil counts were significantly increased at a dose-dependent manner compared with the model group (all P<0.01). The myelosuppression status of AA was significantly reduced in M-, H-PDS-C groups, and hematopoietic cell quantity of bone marrow was more abundant than the model group. The colony numbers of myeloid, erythroid and megakaryocytic progenitor cells in the model group were less than those of the normal mice in bone marrow culture, while, PDS-C therapy enhanced proliferation of hematopoietic progenitor cells by significantly increasing colony numbers (all P<0.01). Furthermore, PDS-C therapy increased peripheral blood CD3+ and CD3+CD4+ cells and reduced CD3+CD8+ cells (P<0.05 or P<0.01). Meanwhile, PDS-C treatment at medium- and high doses groups also increased CD4+CD25+FoxP3+ cells, downregulated T-bet protein expression, and upregulated GATA-3 and FoxP3 protein expressions in spleen cells (P<0.05). CONCLUSION: PDS-C possesses dual activities, promoting proliferation hematopoietic progenitor cells and modulating T lymphocyte immune functions in the treatment of AA model mice.

14.
Nat Commun ; 10(1): 5552, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31804474

RESUMO

Adding small nanoparticles (NPs) into polymer melt can lead to a non-Einstein-like decrease in viscosity. However, the underlying mechanism remains a long-standing unsolved puzzle. Here, for an all-polymer nanocomposite formed by linear polystyrene (PS) chains and PS single-chain nanoparticles (SCNPs), we perform large-scale molecular dynamics simulations and experimental rheology measurements. We show that with a fixed (small) loading of the SCNP, viscosity reduction (VR) effect can be largely amplified with an increase in matrix chain length [Formula: see text], and that the system with longer polymer chains will have a larger VR. We demonstrate that such [Formula: see text]-dependent VR can be attributed to the friction reduction experienced by polymer segment blobs which have similar size and interact directly with these SCNPs. A theoretical model is proposed based on the tube model. We demonstrate that it can well describe the friction reduction experienced by melt polymers and the VR effect in these composite systems.

15.
Br J Pharmacol ; 2019 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-31734944

RESUMO

BACKGROUND AND PURPOSE: Lipid metabolism disorder and disturbed mitochondrial bioenergetics play pivotal roles in the occurrence and development of diabetic kidney disease (DKD). Berberine (BBR) is a kind of alkaloid derived from Chinese herbal medicine. It has multiple therapeutic actions on diabetes mellitus and its complications, including regulation of glucose and lipid metabolism, improvement of insulin sensitivity and alleviation of oxidative damage. Here we investigated the renoprotective effects of BBR. EXPERIMENTAL APPROACH: Combined clinical study in DKD patients with experimental studies in diabetic mice and cultured podocytes, we characterized the energy metabolism profiles based on metabolomics, investigated molecular targets and mechanisms of BBR at regulating mitochondrial function and bioenergetics, and testified the effects of BBR on metabolic alterations in DKD animal model. KEY RESULTS: Metabolomics examination suggested altered mitochondrial fuel usage and generalized mitochondrial dysfunction in DKD patients. BBR intervention potently reversed metabolic disorders, podocyte damage and glomerulosclerosis in db/db mice. Lipid accumulation, excessive generation of mitochondrial reactive oxygen species, mitochondrial dysfunction and insufficient fatty acids oxidation in DKD mouse models and cultured podocytes were significantly suppressed by BBR. The protective mechanism of BBR might involve the activation of peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) signaling pathway, thereby promoting mitochondrial energy homeostasis and fatty acid oxidation in podocytes. CONCLUSION AND IMPLICATIONS: Our research elucidated that PGC-1α-mediated mitochondrial bioenergetics might play a key role in lipid disorder-induced podocyte damage and development of DKD. Restoration of PGC-1α activity and the energy homeostasis by BBR might be a potential therapeutic strategy against DKD.

16.
J Hazard Mater ; : 121557, 2019 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-31735469

RESUMO

Ceramic membranes allow a high filtration flux with a low fouling propensity. Direct filtration of municipal wastewater using flat-sheet ceramic membranes (FSCM) is an attractive and promising technology for wastewater treatment and resource recovery. Urgent need is to determine the fouling behavior of FSCM and its optimal cleaning strategy in direct filtration applications. This study was conducted on pre-coagulation of sewage with FeCl3 (20 mg Fe/L) or polyaluminum chloride (15 mg Al/L), followed by FSCM filtration at around 1.0 m/d (as high as 41.7 L/m2-h in flux). The results showed that the coagulation-FSCM filtration removed chemical oxygen demand and total phosphorus by up to 90.0% and 99.9%, respectively. The pollutants (organics and nutrients) in the sewage influent were concentrated into the sludge for potential resource recovery. Without pre-coagulation, foulants easily entered the membrane pores, causing rapid membrane fouling. Chemically enhanced coagulation greatly decreased the membrane fouling rate by aggregating various foulants into flocs. Fouled membranes were effectively renewed by physical means, such as water flushing and sonication, or a short period of chemical backwashing with NaOH, HCl or oxidizing agents. The study revealed the fouling mechanisms of FSCM in sewage filtration and developed effective cleaning strategies for its long-term operation.

17.
Bioresour Technol ; : 122368, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31735699

RESUMO

Agricultural wastes of cow manure and wheat straw were used to prepare montmorillonite (Mt)-biochars (CMt and WMt) for sorptive removal of 17ß-estradiol (E2) from aqueous solution. The E2 adsorption performance of the two Mt-biochars was investigated using systematic adsorption kinetics and isotherms. The results exhibited that the maximum E2 adsorption amount of CMt was 41.02 mg/g, while it was 62.89 mg/g for WMt. Meanwhile, intraparticle diffusion model demonstrated that intraparticle diffusion was not the only rate-limiting step, both film diffusion and intraparticle diffusion were involved in the diffusion process. Higher pH levels (>10) decreased the adsorption capacities of Mt-biochars for E2. However, the ionic strength and the background electrolytes did not significantly affect adsorption process. Moreover, the two Mt-biochars both exhibited excellent regeneration and reusability. These results provided a potential solution to the recycling of agricultural wastes and the problem of estrogen contaminant.

18.
Peptides ; 123: 170181, 2019 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-31689455

RESUMO

Previous studies suggested that endoplasmic reticulum (ER) stress induced-apoptosis promoted vascular calcification (VC). Interestingly, erythropoietin (EPO), an endogenous glycoprotein, exerts multiple tissue protective effects by inhibiting ER stress and apoptosis. We investigated the role and potential mechanism of EPO on VC in chronic kidney disease (CKD) rats and cultured vascular smooth muscle cells (VSMCs). The calcification model was established by subtotal nephrectomy in vivo or phosphate overload in vitro. The protein level of EPO receptor (EPOR) was increased in the calcified aortas of CKD rats. EPO prevented the reduction of VSMC phenotypic markers, and reversed the increased calcium content and calcium salt deposition in the aortas of CKD rats and cultured calcified VSMCs. The protein levels of activating transcription factor 4 (ATF4) and glucose-regulated protein 94 (GRP94) were upregulated in aortas and VSMCs under calcifying conditions, indicating ER stress activation. EPO treatment of CKD rats or calcified VSMCs downregulated the protein levels of ATF4 and GRP94. Furthermore, ER stress-mediated apoptosis, determined by the protein levels of CCAAT/enhancer-binding protein-homologous protein and cleaved caspase 12, was increased in tunicamycin or calcification media-treated VSMCs, but the increased effect was reversed in EPO-treated groups. The increased apoptotic cells in calcified VSMCs, as indicated by Hoechst staining and flow cytometry, were downregulated by the co-administration of EPO or 4-phenyl butyric acid. In conclusion, EPO might attenuate VC by inhibiting ER stress mediated apoptosis through EPOR signaling.

19.
Neural Netw ; 122: 144-151, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31678798

RESUMO

In this paper, an algorithm based on collective neurodynamic system is investigated for distributed constrained convex optimization, whose objective function is a sum of smooth convex functions and non-smooth L1-norm functions. Inspired by recent advances in distributed convex optimization, the continuous-time and discrete-time distributed optimization algorithms described by collective neurodynamic systems are proposed. In the systems, each of the smooth objective functions is allocated to each node as well as each of the L1-norm functions. However, the L1-norm functions are realized by projection operators. Meanwhile, each node satisfies the local linear and bound constraints. Then a connected network is constituted from all the nodes with consensus to find the optimal solutions.

20.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 36(11): 1123-1126, 2019 Nov 10.
Artigo em Chinês | MEDLINE | ID: mdl-31703141

RESUMO

OBJECTIVE: To explore the genetic cause for abnormal pregnancies through detecting chromosomal copy number variations (CNVs) in abortic tissues by next generation sequencing (NGS). METHODS: NGS technique was used to detect CNVs in abortion tissues. Parental chromosomal karyotypes were predicted based on the results. The aberrant chromosomal segments of the parents were accurately mapped by G-banding karyotyping analysis and fluorescence in situ hybridization (FISH). RESULTS: In addition to numerical chromosomal aberrations, 12 microdeletion/microduplications were detected by NGS. For 8 families where both parents accepted chromosomal karyotyping, 4 carriers of chromosomal abnormalities were identified. One marker chromosome was missed by karyotyping analysis, and a mother was confirmed to carry a cryptic balanced translocation by FISH. CONCLUSION: NGS can facilitate detection of cryptic chromosomal translocations in couples with repeated pregnancy failure and is of great value for detecting abnormal CNVs for its high sensitivity.


Assuntos
Aborto Espontâneo/genética , Aberrações Cromossômicas , Variações do Número de Cópias de DNA , Feminino , Humanos , Hibridização in Situ Fluorescente , Cariotipagem , Pais , Gravidez
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