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1.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(5): 1668-1673, 2020 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-33067971

RESUMO

OBJECTIVE: To investigate the difference of clinical characteristics between young patients(age≤40 years old) and middle-older patients(age>40 years old) with the myeloproliferative neoplasms(MPN). METHODS: The clinical data (gene mutations, peripheral blood routine examinations, imaging examination and past history) of 269 MPN patients was collected and analyzed. RESULTS: In essential thrombocythemia (ET) group, the proportion of triple-negative type in young patients was higher than that in middle-older group, while the peripheral white blood cell(WBC) and platelets(PLT) counts in the first visit were lower. In polycythemia vera (PV) group, the total detection rate of JAK2V617F (80.65%) was lower than that of other research reports. Young patients with PV showed the lower JAK2V617F rate and lower WBC count, compared with the middle-older aged patients. Both CALR and MPL mutations were not found in PV patients. There was only 1 primary myelofibrosis (PMF) patient aged <40 years old. 91.67% of the patients merged splenomegaly and this rate was higher than that of ET or PV patients. It was found that there were a diagnosed familial MPN family and an undiagnosed family, and the youngest patient was only 8 years old. The second-generation gene sequencing detection for them was not carried out. CONCLUSION: Age is an important reference index in the assessment of risks. The MPN patients with different age and types show much difference in gene mutations, peripheral blood cell counts, thrombotic events and sizes of spleen. The onset ages of patients with familial MPN trends to be generational younger.

2.
Nat Methods ; 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33046893

RESUMO

Ensuring reproducibility of results in high-throughput experiments is crucial for biomedical research. Here, we propose a set of computational methods, INTRIGUE, to evaluate and control reproducibility in high-throughput settings. Our approaches are built on a new definition of reproducibility that emphasizes directional consistency when experimental units are assessed with signed effect size estimates. The proposed methods are designed to (1) assess the overall reproducible quality of multiple studies and (2) evaluate reproducibility at the individual experimental unit levels. We demonstrate the proposed methods in detecting unobserved batch effects via simulations. We further illustrate the versatility of the proposed methods in transcriptome-wide association studies: in addition to reproducible quality control, they are also suited to investigating genuine biological heterogeneity. Finally, we discuss the potential extensions of the proposed methods in other vital areas of reproducible research (for example, publication bias and conceptual replications).

3.
Phys Rev Lett ; 125(12): 126801, 2020 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-33016726

RESUMO

Doping a topological insulator (TI) film with transition metal ions can break its time-reversal symmetry and lead to the realization of the quantum anomalous Hall (QAH) effect. Prior studies have shown that the longitudinal resistance of the QAH samples usually does not vanish when the Hall resistance shows a good quantization. This has been interpreted as a result of the presence of possible dissipative conducting channels in magnetic TI samples. By studying the temperature- and magnetic-field-dependence of the magnetoresistance of a magnetic TI sandwich heterostructure device, we demonstrate that the predominant dissipation mechanism in thick QAH insulators can switch between nonchiral edge states and residual bulk states in different magnetic-field regimes. The interactions between bulk states, chiral edge states, and nonchiral edge states are also investigated. Our Letter provides a way to distinguish between the dissipation arising from the residual bulk states and nonchiral edge states, which is crucial for achieving true dissipationless transport in QAH insulators and for providing deeper insights into QAH-related phenomena.

4.
Br J Clin Pharmacol ; 2020 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-33010043

RESUMO

AIMS: Voriconazole is a broad-spectrum antifungal agent for the treatment of invasive fungal infections. There is limited information about the pharmacokinetics and appropriate dosage of voriconazole in patients with liver dysfunction. This study aimed to explore the relationship between voriconazole trough concentration (Ctrough ) and toxicity, identify the factors significantly associated with voriconazole pharmacokinetic parameters and propose an optimised voriconazole dosing regimen for patients with liver dysfunction. METHODS: The study prospectively enrolled 51 patients with 272 voriconazole concentrations. Receiver operating characteristic (ROC) curves were used to explore the relationship between voriconazole Ctrough and toxicity. The pharmacokinetic data was analysed with nonlinear mixed-effects method. Dosing simulations stratified by total bilirubin (TBIL, TBIL-1: TBIL < 51 µmol/L; TBIL-2: 51 µmol/L ≤ TBIL < 171 µmol/L; TBIL-3: TBIL ≥ 171 µmol/L) were performed. RESULTS: ROC curve analysis revealed that voriconazole Ctrough of ≤ 5.1 mg/L were associated with significantly lower the incidence of adverse events. A one-compartment pharmacokinetic model with first-order absorption and elimination was used to describe the data. Population pharmacokinetic parameters of clearance (CL), the volume of distribution (V) and oral bioavailability (F) were 0.88 L/h, 148.8 L and 88.4%, respectively. Voriconazole CL was significantly associated with TBIL and platelet count. The V increased with body weight. Patients with TBIL-1 could be treated with a loading dose of 400 mg every 12 hours (q12h) for first day, followed by a maintenance dose of 100 mg q12h administered orally or intravenously. TBIL-2 and TBIL-3 patients could be treated with a loading dose of 200 mg q12h and maintenance doses of 50 mg q12h or 100 mg once daily (qd) and 50 mg qd orally or intravenously, respectively. CONCLUSIONS: Lower doses and longer dosing intervals should be considered for patients with liver dysfunction. TBIL-based dosing regimens provide a practical strategy for achieving voriconazole therapeutic range and therefore maximizing treatment outcomes.

5.
Chem Commun (Camb) ; 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33057501

RESUMO

Correction for 'Pd-Catalyzed oxidative isomerization of propargylic acetates: highly efficient access to α-acetoxyenones via alkenyl Csp2-O bond-forming reductive elimination from PdIV' by Jun Li et al., Chem. Commun., 2016, 52, 10644-10647, DOI: 10.1039/C6CC04463H.

6.
J Chromatogr Sci ; 2020 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-32893305

RESUMO

Biodiesel consists of various fatty acid methyl esters (FAMEs) that are mainly produced through transesterification of plant oil or animal fat. It is essential for biodiesel to be purified utmostly to meet its product standard before being traded, while the universal purification method has been water washing. However, water washing inevitably causes the residual of FAMEs in wastewater, which represents a loss of industrial profits. For the purpose of determination and monitoring of the FAME profile in wastewater, there is a necessity to develop a fast and reliable approach with small volume of sample in need. Hence, in this study, a combination of dispersive liquid-liquid microextraction (DLLME) and microwave demulsification is applied for the enrichment of residual FAMEs in water, followed by qualitative and quantitative analyses using gas chromatography-mass spectrometry. The results indicate that the optimal extractant in DLLME approach is toluene. And the optimal parameters are 20 mL of water sample, 80 µL of toluene as the extractant, 60 s of ultrasonic irradiation duration, 200 W of microwave power and 2 min of microwave irradiation duration. The standard curves and linear equations obtained with these conditions are used for the quantitative analysis of biodiesel wastewater, which reveals that there was 50.35 mg·L-1 of the total FAME residuals in wastewater. To the best of our knowledge, it is for the first time that the combined technique of DLLME and microwave demulsification is applied in determination of residual FAMEs in water samples. The proposed method corresponds to small volumes of sample and extractant and short analytical period. It also has the potential to be extended to the analysis of other water pollutants.

8.
Mini Rev Med Chem ; 2020 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-32912124

RESUMO

Many plants in the genus Zanthoxylum, belonging to the Rutaceae family, are recorded as folk medicines for the treatment of various diseases, which attracts much attention for their phytochemical and pharmacological activity investigations. Alkaloids are the largest secondary metabolites with structurally diverse types found in this genus and they demonstrate a wide range of biological activities. The aim of this review is to provide a summary on the isolation, classification, and biological properties of the alkaloids from Zanthoxylum species, which also will bring more attention to other researchers for further biological study on alkaloids for the new drug development.

9.
Gynecol Endocrinol ; : 1-5, 2020 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-32936010

RESUMO

BACKGROUND: To compare the efficacy and the tolerability of letrozole combined with oral contraceptives versus oral contraceptives alone in treating endometriosis-related pain. METHODS: A total of 820 women with endometriosis presented with endometriosis-related pain were enrolled with this study. Patients were randomly treated either with letrozole (2.5 mg/day) combined with oral contraceptives (Desogestrel and Ethinylestradiol Tablets) or oral contraceptives (Desogestrel and Ethinylestradiol Tablets) alone for 6 months. Changes in pain symptoms during treatment and in 1 months after treatment, 6-month follow-up and 12-month follow-up were evaluated. Adverse effects of each treatment protocol were recorded. RESULTS: At completion of treatment, the intensity of chronic pelvic pain continued to decrease during treatment and at 1-month after treatment it was significantly lower than at 6-month follow-up and baseline level both in LE + oral contraceptives group (Mean ± SD,1.5 ± 1.4) and in oral contraceptives alone group(Mean ± SD,2.9 ± 1.2).The intensity of chronic pelvic pain and deep dyspareunia was significantly decrease at both 1-month after treatment and 6-month follow-up. CONCLUSIONS: This treatment for endometriosis is a promising new modality that warrants further investigation.

10.
Chem Commun (Camb) ; 56(73): 10607-10620, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32930247

RESUMO

Numerous mononuclear platinum(ii) complexes are non-emissive or weakly emissive under ambient conditions, but the corresponding Pt-M (M = Cu(i), Ag(i), Au(i), etc.) heteronuclear assemblies could become intensely luminescent because of the inhibition of non-radiative relaxation and the promotion of intersystem crossing from singlet to triplet state through Pt-M intermetallic interactions. To this end, the fabrication of specifically structured Pt-M complexes by the use of slightly luminescent homonuclear Pt(ii) precursors provides a promising approach to switching on phosphorescence as well as modulating emission energy and colour. This feature article is aimed at providing some typical examples for attaining highly phosphorescent Pt-M heteronuclear complexes using homonuclear Pt(ii) precursors, focusing on the assembly strategy, the correlation of emissive properties to the structures, and the application of phosphorescence in sensing and light-emitting devices.

12.
BMJ Open ; 10(9): e039711, 2020 09 14.
Artigo em Inglês | MEDLINE | ID: mdl-32928867

RESUMO

OBJECTIVES: High levels of organisational citizenship behaviour can enable nurses to cooperate with coworkers effectively to provide a high quality of nursing care during the outbreak of COVID-19. However, the association between autonomy, optimism, work engagement and organisational citizenship behaviour remains largely unexplored. This study aimed to test if the effect of autonomy on organisational citizenship behaviour through the mediating effects of optimism and work engagement. STUDY DESIGN: This was a cross-sectional study. SETTING: The study was conducted in the Wuhan Jinyintan Hospital in China. PARTICIPANTS: In total, 242 nurses who came from multiple areas of China to work at the Wuhan Jinyintan hospital during the COVID-19 epidemic participated in this study. METHODS: A serial mediation model (model 6) of the PROCESS macro in SPSS was adopted to test the hypotheses, and a 95% CI for the indirect effects was constructed by using Bootstrapping. RESULTS: The autonomy-organisational citizenship behaviour relationship was mediated by optimism and work engagement, respectively. In addition, optimism and work engagement mediated this relationship serially. CONCLUSION: The findings of this study may have implications for improving organisational citizenship behaviour. The effects of optimism and work engagement suggest a potential mechanism of action for the autonomy-organisational citizenship behaviour linkage. A multifaceted intervention targeting organisational citizenship behaviour through optimism and work engagement may help improve the quality of nursing care among nurses supporting patients with COVID-19.


Assuntos
Comportamento Cooperativo , Infecções por Coronavirus/enfermagem , Enfermeiras e Enfermeiros/psicologia , Otimismo/psicologia , Pneumonia Viral/enfermagem , Autonomia Profissional , Engajamento no Trabalho , Atitude do Pessoal de Saúde , Betacoronavirus , China , Humanos , Pandemias , Comportamento Social , Inquéritos e Questionários
13.
Artigo em Inglês | MEDLINE | ID: mdl-32981314

RESUMO

Adenosine triphosphate (ATP) is mainly produced in mitochondria and plays an important role in lots of pathological processes such as colitis. Unfortunately, to date, few suitable fluorescence probes have been developed for monitoring the ATP level in colitis. Herein, a fluorescence nanoprobe named NIR@ZIF-90 is proposed and prepared by encapsulating a rhodamine-based near-infrared (NIR) dye into zeolitic imidazolate frameworks (ZIF-90). The nanoprobe is nonfluorescent because the emission of NIR is suppressed by the encapsulation, while in the presence of ATP, the framework of ZIF-90 is dissembled to release NIR and thus NIR fluorescence at 750 nm is observed. The nanoprobe shows high sensitivity to ATP with a 72-fold increase and excellent selectivity to ATP over other nucleotides. Moreover, with low cytotoxicity and good mitochondria-targeted ability, NIR@ZIF-90 is used to image ATP in colorectal cancer cells (HCT116). In addition, due to the NIR emission, the nanoprobe is further employed to successfully monitor the ATP level in a colitis mouse model. To the best of our knowledge, the nanoprobe is the first example to study colitis in vivo with the guidance of ATP, which will provide an efficient tool for understanding colitis.

14.
Respir Care ; 2020 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-32962996

RESUMO

BACKGROUND: Low airway surface pH is associated with many airway diseases, impairs antimicrobial host defense and worsens airway inflammation. Inhaled Optate is designed to safely to raise airway surface pH and is well-tolerated in humans. Raising intracellular pH partially prevents activation of SARS-CoV-2 in primary normal human airway epithelial (NHAE) cells, decreasing viral replication by several mechanisms. METHODS: Here, we grew primary normal human airway epithelial (NHAE) cells from healthy subjects, infected them with SARS-CoV-2 (isolate USA-WA1/2020), and used clinical Optate at concentrations used in humans in vivo to determine whether it would prevent viral infection and replication. Cells were pre-treated with Optate or placebo prior to infection (MOI of 0.1) and viral replication was determined by plaque assay and nucleocapsid (N) protein levels. Healthy human subjects also inhaled Optate as part of a Phase 2a safety trial. RESULTS: Optate almost completely prevented viral replication at each time point between 24 and 120 hours, relative to placebo, both by plaque assay and by N protein expression (p < 0.001). Mechanistically, Optate inhibited expression of major endosomal trafficking genes and raised NHAE intracellular pH. Optate had no effect on NHAE cell viability at any time point. Inhaled Optate was well tolerated in 10 normal subjects, with no change in lung function, vital signs or oxygenation. CONCLUSIONS: Inhaled Optate may be well-suited for a clinical trial in patients with a pulmonary SARS-CoV-2 infection. However, it is vitally important for patient safety that formulations designed for inhalation with regards to pH, isotonicity and osmolality be used. An inhalational treatment that safely prevents SARS-CoV-2 viral replication could be helpful for treating patients with pulmonary SARS-CoV-2 infection.

15.
Artigo em Inglês | MEDLINE | ID: mdl-32929955

RESUMO

Cadmium sulfide (CdS) as one of the most common visible-light-responsive photocatalysts has been widely investigated for hydrogen generation. However, its low solar-hydrogen conversion efficiency caused by fast carrier recombination and poor catalytic activity hinders its practical applications. To address this issue, we develop a novel and highly efficient nickel-cobalt phosphide and phosphate cocatalyst-modified CdS (NiCoP/CdS/NiCoPi) photocatalyst for hydrogen evolution. The dual-cocatalysts were simultaneously deposited on CdS during one phosphating step by using sodium hypophosphate as the phosphorus source. After the loading of the dual-cocatalysts, the photocurrent of CdS significantly increased, while its electrical impedance and photoluminescence emission dramatically decreased, which indicates the enhancement of charge carrier separation. It was proposed that the NiCoP cocatalyst accepts electrons and promotes hydrogen evolution, while the NiCoPi cocatalyst donates electrons and accelerates the oxidation of sacrificial agents (e.g., lactic acid). Consequently, the visible-light-driven hydrogen evolution of this composite photocatalyst greatly improved. The dual-cocatalyst-modified CdS with a loading content of 5 mol % showed a high hydrogen evolution rate of 80.8 mmol·g-1·h-1, which was 202 times higher than that of bare CdS (0.4 mmol·g-1·h-1). This is the highest enhancement factor for metal phosphide-modified CdS photocatalysts. It also exhibited remarkable stability in a continuous photocatalytic test with a total reaction time of 24 h.

16.
Asian J Surg ; 2020 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-32988708

RESUMO

Long-term overall survival (OS) after liver resection for non-cirrhotic hepatocellular carcinoma (NCHCC) has been reported recently. The aim of this study was to review outcomes systematically and analyze risk factors for survival after surgical resection for HCC without cirrhosis. A literature search was performed of the PubMed and Embase databases for papers published between January 1995 and October 2012, which focused on hepatic resection for HCC without underlying cirrhosis. Cochrane systematic review methodology was used for this review. Outcomes were OS, operative mortality and disease-free survival (DFS). Pooled hazard ratios (HR) were calculated using the random effects model for parameters considered as potential prognostic factors. Totally, 26 retrospective case series were eligible for inclusion. The 1-, 3- and 5-year OS rate after surgical resection of NCHCC ranged from 62% to 100%, 46.3%-78.0%, and 30%-64%, respectively. The corresponding DFS rates ranged from 48.7% to 84%, 31.0%-66.0%, and 24.0%-58.0%, respectively. Five variables were related to poor survival: multiple tumors (HR 1.68, 95%CI 1.25-2.11); larger tumor size (HR 2.66, 95%CI 1.69-3.63); non-clear resection margin (R0 resection) (HR 3.52, 95%CI 1.63-5.42); poor tumor stage (HR 2.61, 95%CI 1.64-3.58); and invasion of the lymphatic vessels (HR 4.85, 95%CI 2.67-7.02). In sum, hepatic resection provides excellent OS rates for patients with NCHCC, and results have tended to improve recently. Risk factors for poor prognosis comprise multiple tumors, lager tumor size, non-R0 resection and invasion of the lymphatic vessels.

17.
Inorg Chem ; 2020 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-32959655

RESUMO

An ethylammonium-templated indium sulfide, [CH3CH2NH3]6In8S15 (InS-2), featuring anionic layers perforated with large, 24-membered rings that facilitate the accommodation of hydrated Sr2+ ions is reported. InS-2 exhibits an excellent adsorption performance toward Sr2+ with a top-ranked capacity (qm = 143.29 mg g-1), rapid kinetics, wide pH durability (3-14), ß- and γ-radiation resistances, and a facile elution.

18.
J Orthop Res ; 2020 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-32940927

RESUMO

The association between proximal tibiofibular joint (PTFJ) and knee osteoarthritis (OA) has been understudied. The objective of this study was to determine whether the morphology of PTFJ has predictive value for the risk of total knee replacement (TKR). Case knees from Osteoarthritis Initiative participants with TKR at 24-60 months follow up (cases) were 1:1 matched with control knees (no TKR throughout 60 months) by baseline age, sex, and Kellgren-Lawrence grade. PTFJ morphological parameters, including coronal inclination angle (angle α), sagittal inclination angle (angle ß), fibular contacting area (S), load-bearing area (Sτ), lateral stress-bolstering area (Sφ), and posterior stress-bolstering area (Sυ) were assessed using coronal and sagittal magnetic resonance imaging (MRI), respectively. Associations of the morphological measures at baseline and the time point before TKR (T0 ) and their changes with TKR risks were examined using conditional logistic regression analyses. Two hundred and twenty-three knees of 193 participants received TKR between 12 and 60 months and therefore were matched with 223 control knees. Of these, 173 paired knees had MRI readings available both at baseline and T0 time point. While baseline angle α was positively associated with TKR risk, other measures at baseline and all measures at T0 were not significantly associated with TKR risk. Changes in S, Sτ, and Sυ were significantly and negatively associated with the risk of TKR (ΔS, odds ratio [OR] = 0.38, 95% confidence interval [CI]: 0.19-0.76; ΔSτ, OR = 0.37, 95% CI: 0.16-0.87; ΔSυ, OR = 0.22, 95% CI: 0.08-0.62, respectively). This data shows that morphological changes of PTFJ predict the risk of TKR, suggesting PTFJ may play a role in knee OA.

19.
Chin J Dent Res ; 23(3): 183-189, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32974618

RESUMO

OBJECTIVE: To design and fabricate 3D-printed rigid constraint guides for the tooth preparation for laminate veneers and to evaluate the accuracy of guide-assisted preparation. METHODS: Twenty maxillary right central incisor resin artificial teeth were randomly divided into two equal groups and prepared for laminate veneers. Tooth preparations were performed, assisted by guides in the test group and by depth gauge burs in the control group, and both were finished by freehand operation. The typodonts were 3D scanned before preparation, after initial preparation and after final preparation. The tooth preparation depths at each step, including initial preparation depth, final preparation depth and loss of tooth tissue during polishing, were measured by 3D deviation analysis. Statistical analyses were conducted to investigate differences. RESULTS: The initial preparation depth was 0.488 mm (median, quartile 0.013 mm) in the test group and 0.521 mm (median, quartile 0.013 mm) in the control group. A statistically significant difference was found between them (P < 0.05). The final preparation depth in the test group (0.547 ± 0.029 mm) was significantly less than that in the control group (0.599 ± 0.051 mm) (P < 0.05), and closer to the predesigned value (0.5 mm). There was no statistically significant difference in the loss of tooth tissue during polishing between the test group (0.072 ± 0.023 mm) and the control group (0.089 ± 0.038 mm) (P > 0.05). CONCLUSION: In maxillary central incisors, the tooth preparation for laminate veneers could be conducted using 3D-printed rigid constraint guides, the accuracy of which is better than that of depth gauge burs.


Assuntos
Porcelana Dentária , Facetas Dentárias , Incisivo , Lasers , Preparo do Dente
20.
Sci Rep ; 10(1): 15502, 2020 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-32968187

RESUMO

In vivo and vitro evidence indicates that ornithine and its related metabolic products play a role in tumor development. Whether ornithine is associated with breast cancer in humans is still unclear. We examined the association between circulating ornithine levels and breast cancer in females. This 1:1 age-matched case-control study identified 735 female breast cancer cases and 735 female controls without breast cancer. All cases had a pathological test to ascertain a breast cancer diagnosis. The controls were ascertained using pathologic testing, clinical examinations, and/or other tests. Fasting blood samples were used to measure ornithine levels. The average age for cases and controls were 49.6 years (standard deviation [SD] 8.7 years) and 48.9 years (SD 8.7 years), respectively. Each SD increase in ornithine levels was associated with a 12% reduction of breast cancer risk (adjusted odds ratio [OR] 0.88; 95% confidence interval [CI] 0.79-0.97). The association between ornithine and breast cancer did not differ by pathological stages of diagnosis or tumor grades (all P for trend > 0.1). We observed no effect measure modification by molecular subtypes (P for interaction = 0.889). In conclusion, higher ornithine levels were associated with lower breast cancer risk in females.

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