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1.
Rapid Commun Mass Spectrom ; 37(1): e9419, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36260057

RESUMO

Metabolism is undoubtedly significantly correlated with the efficacy and safety of traditional Chinese medicine. In clinic, Qi-Yu-San-Long decoction (QYSLD) has achieved good results in the treatment of non-small-cell lung cancer (NSCLC). Nevertheless, a detailed understanding of the compounds (prototypes and metabolites) of QYSLD and its dynamic metabolic profile in plasma has not been revealed. METHODS: In this study, a rapid and sensitive method based on ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF/MSE ), combined with a four-step analysis strategy, was established to investigate QYSLD metabolic profile in rat plasma. RESULTS: In all, 101 xenobiotics (41 prototypes and 60 QYSLD-related metabolites) were identified in rat plasma. The research uncovered metabolic profiles of alkaloids, saponins, flavonoids, iridoids, anthraquinones, and phenylpropanoids of QYSLD in rat plasma. The dynamic changes in these xenobiotics were also observed at different time intervals. At 0.5 h after oral administration, only 15 prototypes and 11 metabolites were detected. Within 24 h, 4 prototypes and 20 metabolites can still be detected. Four prototypes and 10 metabolites had the phenomenon of emergence-disappearance-reappearance in vivo. CONCLUSION: In rat plasma, 101 xenobiotics of QYSLD were identified and their dynamic metabolic profiles were systematically delineated, which laid a material basis for further research of the pharmacodynamic substances of QYSLD inhibiting NSCLC.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Medicamentos de Ervas Chinesas , Neoplasias Pulmonares , Ratos , Animais , Espectrometria de Massas em Tandem/métodos , Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/química , Ratos Sprague-Dawley , Cromatografia Líquida , Xenobióticos , Administração Oral
2.
Food Chem ; 403: 134287, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36183467

RESUMO

Tobramycin (TOB), as a widely used antibiotic, poses severe unpredictable risks to ecology and health. Here, a novel photoelectrochemical (PEC) aptasensor based on a "turn-off" PEC mode was constructed for TOB detection. The working electrode could be modified by g-C3N4/WC/WO3 composites and TOB aptamer probes in turn. The TOB aptamer probes could anchor on the g-C3N4/WC/WO3 through π-π stacking interaction to avoid interference from other modifications. When TOB was captured by the aptamer probes anchored on the modified fluorine-doped tin oxide (FTO) electrode, a decreased photocurrent was obtained owing to steric hindrance and hinder electron transfer. Under optimal conditions, 0.005-5 ng mL-1 of TOB could be detected with a detection limit as low as 2 pg mL-1. Meanwhile, actual samples were investigated as well. The proposed sensor shows high specificity, satisfactory detectability, great reproducibility, and may provide a new thought for detecting other pollutants.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Técnicas Eletroquímicas , Tobramicina , Reprodutibilidade dos Testes , Eletrodos , Limite de Detecção
3.
Sci Total Environ ; 855: 158982, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36155033

RESUMO

With rapid economic development, an increasing number of people suffer from mental health diseases, which are gradually receiving the attention of society. However, basic data from surveys of mental disorders are limited. Composite influent samples were collected from 26 wastewater treatment plants in 23 major cities in China. The concentrations of the psychoactive drugs diphenhydramine, fluoxetine, doxepin, imipramine, sulpiride, zolpidem, carbamazepine, and flunitrazepam in the wastewater were determined. The detection frequency of diphenhydramine, sulpiride, and carbamazepine was close to 100 %, whereas that of the compounds was lower than 35 %. Carbamazepine had the highest mean consumption (31.1 mg/d/1000 people), followed by diphenhydramine (10.4 mg/d/1000 people) and sulpiride (11.3 mg/d/1000 people). Wastewater-based epidemiology (WBE) estimates of the average use of the three drugs were lower than those from the drug statistics data. Consumption of diphenhydramine in northern China was higher than that in southern China. A correlation analysis of psychotropic and illicit drugs revealed a correlation between sulpiride and heroin use, which may be related to the adverse effects of sulpiride treatment after heroin withdrawal. Psychotropic drug use is associated with both economic and social factors. We found associations between the use of the three drugs and age, occupation, and obesity, which are risk factors for mental disorders. The results showed that the monitoring of psychotropic drug using WBE has a certain reference value for public health care and for improving the understanding of mental disorders.


Assuntos
Vigilância Epidemiológica Baseada em Águas Residuárias , Poluentes Químicos da Água , Humanos , Cidades , Heroína/análise , Sulpirida/análise , Poluentes Químicos da Água/análise , Águas Residuárias/análise , Psicotrópicos/análise , China/epidemiologia , Carbamazepina/análise , Difenidramina/análise
4.
Comput Human Behav ; 138: 107424, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35945974

RESUMO

Background: There has been growing evidence of comorbidity between internet addiction and depression in youth during the COVID-19 period. According to the network theory, this may arise from the interplay of symptoms shared by these two mental disorders. Therefore, we examined this underlying process by measuring the changes in the central and bridge symptoms of the co-occurrence networks across time. Methods: A total of 852 Chinese college students were recruited during two waves (T1: August 2020; T2: November 2020), and reported their internet addiction symptoms and depressive symptoms. Network analysis was utilized for the statistical analysis. Results: The internet addiction symptoms "escape" and "irritable," and depression symptoms "energy" and "guilty" were the central symptoms for both waves. At the same time, "guilty" and "escape" were identified as bridge symptoms. Notably, the correlation between "anhedonia" and "withdrawal" significantly increased, and that between "guilty" and "escape" significantly decreased over time. Conclusions: This study provides novel insights into the central features of internet addiction and depression during the two stages. Interestingly, "guilty" and "escape," two functions of the defense mechanism, are identified as bridge symptoms. These two symptoms are suggested to activate the negative feedback loop and further contribute to the comorbidity between internet addiction and depression. Thus, targeting interventions on these internalized symptoms may contribute to alleviating the level of comorbidity among college students.

5.
Ultrasonics ; 127: 106837, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36075161

RESUMO

In this article, a novel ultrasound computed tomography (USCT) reconstruction algorithm for breast imaging is proposed. This algorithm is based on an ultrasound propagation model, the refract-ray model (RRM). In this model, the field of imaging is assumed as piecewise homogenous and is divided into several regions. The ultrasound propagation paths are considered polylines that only refract at the borders of the regions. The edge information is provided by B-mode imaging. Both simulations and experiments are implemented to validate the proposed algorithm. Compared with the traditional bent-ray model (BRM), the time of reconstructions using RRM decreases by over 90 %. In simulations, the imaging qualities for RRM and BRM are comparable, in terms of the root mean square error, the Tenengrad value, and the deformation of digital phantom. In the experiments, a cylindrical agar phantom is imaged using a customized imaging system. When imaging using RRM, the estimate of the phantom radius is about 0.1 mm in error, while it is about 0.3 mm in error using BRM. Moreover, the Tenengrad value of the result using RRM is much higher than that using BRM (9.76 compared to 0.79). The results show that the proposed algorithm can better delineate the phantom within a water bath. In future work, further experimental work is required to validate the method for improving imaging quality under breast-mimicking imaging conditions.


Assuntos
Processamento de Imagem Assistida por Computador , Tomografia Computadorizada por Raios X , Ágar , Algoritmos , Processamento de Imagem Assistida por Computador/métodos , Imagens de Fantasmas , Tomografia Computadorizada por Raios X/métodos , Água
6.
Chemosphere ; 310: 136937, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36273608

RESUMO

The fabrication of metal-carbon hybrids with heteroatom doping from manganese-metal organic frameworks (MOFs) has rarely been reported for peroxymonosulfate (PMS) activation. In this work, novel MnOx@N-doped carbon (MnOx@NC) nanosheets were prepared using 2D manganese-1,4 benzenedicarboxylic acid-based MOFs (Mn-MOFs) and different proportions of graphitic carbon nitride (g-C3N4, additional N source and carbon source) to activate PMS for sulfamethoxazole (SMX) removal. The polarization difference induced by Mn-N coordination during the carbonization process made C an electron-poor center and Mn an electron-rich center, thus providing more Mn(II) for PMS activation. Benefiting from the highest Mn(II) content, the most uniform and exposed MnOx active sites, abundant N active species and rich defective sites, MnOx@NC-20 showed excellent degradation (72.9% within 5 min) and mineralization performance (47.40% within 60 min) for SMX. Nonradical and radical processes worked together in MnOx@NC-20/PMS/SMX system, where singlet oxygen (1O2) dominated the degradation of SMX. N-doped carbon not only exhibited dragging and protection effects on MnOx, but also provided adsorption sites for PMS and pollutants, thus reducing their migration distance. Moreover, the electrons of organic substrates could be captured by the electron-poor carbon layer and then transported to the electron-rich Mn center, thus improving the utilization efficiency of PMS and the redox of Mn. This study provides a facile optimization method to prepare MOFs-derived carbon catalysts with improved stability and catalytic performance.


Assuntos
Carbono , Estruturas Metalorgânicas , Carbono/química , Elétrons , Manganês , Peróxidos/química , Sulfametoxazol
7.
Ultrasound Med Biol ; 49(1): 289-308, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36283938

RESUMO

Shear wave velocity (SWV) reconstruction based on time-of-flight (TOF) is widely adopted to realize shear wave elasticity imaging (SWEI). It typically breaks down the reconstruction of a SWV image into many kernels and treats them independently. We hypothesized that information exchange among kernels improves the performance of SWEI. Therefore, we propose the approach of iterative re-weighted least squares based on inter-kernel communication (IKC-IRLS). We also hypothesized that time-to-peak (TTP) is superior to cross-correlation (CC) in visualizing small targets because TTP uses higher shear wave frequencies than CC. To examine the hypotheses, IKC-IRLS was combined with TTP data and compared with four established methods. The five methods were tested by imaging several small-size stiff targets (2.5, 4.0 and 6.4 mm in diameter) using different kernel sizes in the simulation and real experiments. The results indicate that the IKC-IRLS approach can mitigate speckle noise and is robust to TTP outliers. Consequently, the proposed method achieves the highest contrast-to-noise ratio and the lowest mean absolute percentage error of target in almost all tested cases.


Assuntos
Técnicas de Imagem por Elasticidade , Técnicas de Imagem por Elasticidade/métodos , Imagens de Fantasmas , Simulação por Computador , Elasticidade
8.
Food Chem ; 404(Pt A): 134583, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36257274

RESUMO

Umami peptides are an important taste substance in fermented foods. However, in the absence of known microbiota-derived umami peptides, the understanding of the umami mechanism remains unclear. Tetragenococcus halophilus, a dominant fermentation bacteria, may be an important source of umami peptides. Accordingly, T. halophilus fermentation broth was fractioned by ethanol precipitation, gel chromatography, and reverse phase-high performance liquid chromatography. The isolated peptide fraction with the most intense umami taste was screened by amino acid composition and sensory analyses. Finally, three novel microbiota-derived peptides (DFE, LAGE, and QLQ) were identified, synthesized, and characterized for taste. Among them, only DFE had umami and umami-enhancing abilities improving multiple tastes. Molecular docking studies indicated that DEF binds to T1R1/T1R3 receptors through hydrogen bonding and electrostatic interactions involving receptor residues Ser332, Ser256, ASN41, His125, etc. This study highlights the critical role of microbiota-derived peptides in the umami taste of fermented foods.


Assuntos
Microbiota , Paladar , Simulação de Acoplamento Molecular , Peptídeos/química , Aminoácidos , Receptores Acoplados a Proteínas G/metabolismo
9.
Food Chem ; 402: 134222, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36130432

RESUMO

It is significant to develop low-cost, sensitive, and portable sensing platform for point-of-care testing of ascorbic acid (AA) in food quality evaluation. Herein, a smart point-of-care (SPOC) sensor, consisting of fluorescent paper chip, 3D printed-accessories and smartphone, was developed for ultrasensitive and visual quantitative detection of AA. Fluorescent paper chip was made through printing silicon doped carbon dots (SiCDs)-Fe3+ as "ink" onto filter paper, in which SiCDs emitting strong fluorescence signal quenched by Fe3+, and then recovered by AA owing to releasing -NH2/-OH and introducing defects on SiCDs. The smartphone with an application named "AA-Tester" was established to analyze AA and exhibited high sensitivity with low detection limits of 18.12 nmol/L. HPLC method was used to verify the SPOC sensor and obtained satisfactory accuracy with relative standard deviations of 0.79-2.31 %. The portable sensing platform integrated fluorescence sensor with smartphone-device will be favorable for ultrasensitive and on-the-spot detection of AA.


Assuntos
Ácido Ascórbico , Pontos Quânticos , Espectrometria de Fluorescência/métodos , Smartphone , Limite de Detecção , Corantes Fluorescentes , Silício , Carbono
10.
Gene ; : 147087, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36464167

RESUMO

The study aimed to screen for the causative variants in Chinese patients with suspected retinitis pigmentosa (RP). A cohort of 75 unrelated Chinese patients with a clinical diagnosis of RP and their available family members were enrolled in this study. Genomic DNA of all subjects was extracted and whole-exome sequencing (WES) was applied. Candidate variants were identified, and minigene assays were conducted to evaluate the pathogenicity of novel splicing variants. Totally, the diagnostic yield was 44% (33/75) and 16 novel variants that had not been reported previously were found. Among the genetically solved 33 cases, 31 patients were identified as carrying causative variants of RP and 2 patients carried pathogenic variants implicated in other retinal diseases. USH2A, CYP4V2, and RPGR were the most common causative genes, accounting for about half of the genetically solved cases. Moreover, minigene assays validated that the novel splicing variants were detrimental. Additionally, 9 patients carried a single deleterious heterozygous variant in 6 genes with autosomal recessive hereditary patterns, and no corresponding copy number variants (CNVs) was detected. The findings of this study revealed the genetic landscape of RP in China and provided guidance for clinicians.

11.
Gen Relativ Gravit ; 54(12): 156, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36465478

RESUMO

Detection of a gravitational-wave signal of non-astrophysical origin would be a landmark discovery, potentially providing a significant clue to some of our most basic, big-picture scientific questions about the Universe. In this white paper, we survey the leading early-Universe mechanisms that may produce a detectable signal-including inflation, phase transitions, topological defects, as well as primordial black holes-and highlight the connections to fundamental physics. We review the complementarity with collider searches for new physics, and multimessenger probes of the large-scale structure of the Universe.

12.
Artigo em Inglês | MEDLINE | ID: mdl-36472768

RESUMO

PURPOSE: Pancreatic ductal adenocarcinoma (PDAC) is currently one of the leading causes of cancer death worldwide. Therefore, building further subgroups as well as enabling individual patient therapy and diagnostics are needed. X-linked inhibitor of apoptosis protein (XIAP) is known to modulate apoptotic and inflammatory pathways. Its expression was found to correlate with patients' survival in other tumor entities. This study aims to examine the role of XIAP in patients with PDAC in relation to the inflammatory microenvironment. METHODS: The PANCALYZE multicenter study group included 257 patients with PDAC. Paraffin-embedded tumor samples were stained immunohistochemically for CD3, CD20, CD38, CD56, CD66b, CD117, and CD163 and XIAP. These stainings were further analyzed digitally with QuPath and survival analyses were done. RESULTS: XIAP-positive patients with T-cell, respectively, neutrophil enriched tumors survived significantly longer compared to XIAP-negative patients (CD3: 37.6 vs. 24.6 months, p = 0.028; CD66b: 34.1 vs. 14.9 months, p = 0.027). Additionally, XIAP-positive patients showed better survival in the lymph node-negative population (48.4 vs. 24.2 months, p = 0.019). Regarding the total population, our findings did not show a correlation between XIAP expression and survival. In multivariate cox regression analyzes XIAP proves to be an independent factor for better survival in the identified subgroups (CD3: p = 0.043; CD66b: p = 0.012, N0: p = 0.040). CONCLUSION: We found XIAP-positive subgroups with significantly better survival in patients with PDAC in T-cell-rich, neutrophil-rich, or lymph node-negative cohorts. This could lead to further individualized cancer treatment with less aggressive therapy protocols for XIAP-positive tumors or more intensive follow-up for XIAP-negative tumors.

13.
Zhen Ci Yan Jiu ; 47(11): 975-82, 2022 Nov 25.
Artigo em Chinês | MEDLINE | ID: mdl-36453674

RESUMO

OBJECTIVE: To observe the effect of electroacupuncture (EA) on the ocular surface inflammation and α7 nicotinic acetylcholine receptor (α7nAChR) / nuclear factor kappa-B (NF-κB) p65 signal pathway in guinea pigs with dry eye, so as to explore its underlying mechanism. METHODS: A total of 32 male British tricolor short haired guinea pigs were randomized into blank control, model, EA and sham acupuncture groups, with 8 guinea pigs in each group. The dry eye model was established by subcutaneous injection of scopolamine hydrobromide solution (0.6 mg/0.2 mL each time, 4 times a day for 10 days). Guinea pigs of the EA group was treated with EA at bilateral "Cuanzhu" (BL2) and "Taiyang" (HN5), and manual acupuncture at bilateral "Jingming" (BL1), "Sizhukong" (SJ23), "Tongziliao" (GB1) for 15 min, once daily for 14 days. For animals of the sham acupuncture group, a blunt needle was used to prick the skin surface of the acupoints, the acupoint selection and stimulation time were the same as those in the EA group. Before and after modeling and after the intervention, the breakup time (BUT) of lacrimal film, sodium fluorescein coloring (Fl) state of corneal epithelium and phenol red thread (PRT) moist length were recorded for assessing the severity of dry eye. The density of activated immune cells around the corneal epithelial stromal cells was determined by corneal confocal microscopy. The contents of interleukin-4 (IL-4), IL-6, IL-10, tumor necrosis factor α (TNF-α) in the cornea and lacri-mal gland tissues were determined by ELISA, and the expression levels of α7nAChR and NF-κB p65 in the cornea and lacrimal gland were detected by immunohistochemistry and Western blot, separately. RESULTS: Compared with the blank control group, the corneal Fl, density of activated immune cells of corneal epithelium, contents of IL-6, IL-10 and TNF-α in both corneal and lacrimal gland tissues, NF-κB p65 cell positive rate and protein expression of lacrimal gland and corneal tissues were significantly increased (P<0.01, P<0.05), while the BUT, PRT and lacrimal gland α7nAChR cell positive rate considerably decreased (P<0.01) in the model group. In comparison with the model group, the level of corneal Fl, density of the activated immune cells of corneal epithelium, contents of corneal and lacrimal IL-6 and TNF-α, and corneal and lacrimal NF-κB p65 cell positive rates and protein expressions were remarkably down-regulated in the EA group (P<0.01, P<0.05), rather than in the sham acupuncture group (P>0.05) except content of corneal IL-10, lacrimal NF-κB p65 cell positive rate and lacrimal α7nAChR protein expression, whereas the levels of BUT, PRT, corneal and lacrimal IL-10 and corneal and lacrimal α7nAChR cell positive rates and protein expressions significantly up-regulated in the EA group (P<0.01, P<0.05), rather than in the sham acupuncture group (P>0.05) except corneal TNF-α and corneal NF-κB p65 protein expression. CONCLUSION: EA can improve corneal and lacrimal function in dry eye guinea pigs, which may be associated with its actions in increasing the expression of α7nAChR, inhibiting the nuclear translocation of NF-κB, and reducing the activated immune cells and inflammatory reaction.


Assuntos
Terapia por Acupuntura , Síndromes do Olho Seco , Aparelho Lacrimal , Masculino , Cobaias , Animais , NF-kappa B/genética , Receptor Nicotínico de Acetilcolina alfa7/genética , Interleucina-10 , Fator de Necrose Tumoral alfa , Interleucina-6 , Síndromes do Olho Seco/genética , Síndromes do Olho Seco/terapia , Transdução de Sinais , Inflamação/genética , Inflamação/terapia
14.
Br J Pharmacol ; 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36455594

RESUMO

BACKGROUND AND PURPOSE: Liver fibrosis is an important risk factor for progression from chronic liver injury to hepatocellular carcinoma. Clinically, there is a serious lack of therapeutic drugs for liver fibrosis. Previous studies have confirmed that GL-V9, a new synthesized flavonoid derivative, exhibits anti-inflammatory activity, but whether it has anti-hepatic fibrosis remains unclear. This study aims to investigate the anti-fibrotic activities and potential mechanisms of GL-V9. EXPERIMENTAL APPROACH: Bile duct ligation (BDL) and carbon tetrachloride (CCl4 ) challenge were used to assess anti-fibrotic effects of GL-V9 in vivo. Mouse primary hepatic stellate cells (pHSCs) and human HSC line LX2 also served as an in vitro liver fibrosis model. Cellular functions and molecular mechanism were analyzed using senescence-associated beta-galactosidase staining, real-time PCR, western blotting, immunofluorescence and co-immunoprecipitation. KEY RESULTS: GL-V9 attenuated hepatic histopathological injury, collagen accumulation and decreased the expression of fibrotic genes in vivo. In vitro, GL-V9 promoted senescence and inhibited the expression of fibrogenic genes in HSCs. Mechanistic studies revealed that GL-V9 induced senescence by upregulating GATA4 expression in HSCs. Further studies confirmed that GL-V9 stabilized GATA4 by promoting autophagic degradation of P62. CONCLUSION AND IMPLICATIONS: GL-V9 exerted potent anti-fibrotic effect both in vivo and in vitro by stabilizing GATA4, thereby promoting the senescence of HSCs, avoiding its activation and ultimately inhibiting liver fibrosis. This indicated that the flavonoid GL-V9 is a potential therapeutic candidate for the treatment of liver fibrosis.

15.
Radiother Oncol ; 177: 113-120, 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36336111

RESUMO

PURPOSE: To determine the differences in supraclavicular lymph node metastasis between esophageal cancer (EC) and nasopharyngeal cancer (NPC) and explore the feasibility of differential supraclavicular clinical target volume (CTV) contouring between these two diseases based on the involvement of different fascial spaces. MATERIALS AND METHODS: One hundred patients with supraclavicular nodes positive for EC or NPC were enrolled, and their pre-treatment images were reviewed. The distribution patterns of nodes between the two diseases were compared in the context of node levels defined by the 2017 Japanese Esophageal Society and 2013 International Consensus on Cervical Lymph Node Level Classification. Grouping supraclavicular nodes based on sub-compartments formed by the cervical fascia was discussed, and the feasibility of differential CTV contouring based on the differences in the involvement of these sub-compartments between EC and NPC was explored. RESULTS: The 2013 Consensus on cervical node levels and 2017 Japanese Esophageal Society node station could not practically guide supraclavicular CTV contouring. We divided the supraclavicular space into six sub-compartments: the para-esophageal space (PES), carotid sheath space (CSS), sub-thyroid pre-trachea space (STPTS), pre-vascular space (PVS), and vascular lateral space (VLS) I and II. EC mainly spread to the PES, STPTS, CSS, and VLS I, whereas NPC tended to spread to the CSS, VLS I, and VLS II. These combinations of sub-compartments may help constitute the supraclavicular CTVs for EC and NPC. CONCLUSIONS: The fascia anatomy-based sub-compartments sufficiently distinguished metastasis to the supraclavicular space between EC and NPC, thus facilitating differential CTV contouring between these two diseases.

16.
Methods ; 208: 42-47, 2022 12.
Artigo em Inglês | MEDLINE | ID: mdl-36341922

RESUMO

The adaptor proteins play a crucially important role in regulating lymphocyte activation. Rapid and efficient identification of adaptor proteins is essential for understanding their functions. However, biochemical methods require not only expensive experimental costs, but also long experiment cycles and more personnel. Therefore, a computational method that could accurately identify adaptor proteins is urgently needed. To solve this issue, we developed a classifier that combined the support vector machine (SVM) with the composition of k-Spaced Amino Acid Pairs (CKSAAP) and the amino acid composition (AAC) to identify adaptor proteins. Analysis of variance (ANOVA) was used to select the optimized features which could generate the maximum prediction performance. By examining the proposed model on independent data, we found that the 447 optimized features could achieve an accuracy of 92.39% with an AUC of 0.9766, demonstrating the powerful capabilities of our model. We hope that the proposed model could provide more clues for studying adaptor proteins.


Assuntos
Biologia Computacional , Máquina de Vetores de Suporte , Biologia Computacional/métodos , Aminoácidos/metabolismo , Análise de Variância
17.
ACS Appl Mater Interfaces ; 14(47): 53421-53432, 2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-36384285

RESUMO

Developing high-performance protective materials is important for soldiers and civilians who are exposed to the atmosphere of highly toxic chemical warfare agents (CWAs). Polyelectrolyte membranes are promising candidates with excellent chemical resistance and moisture permeability, but they cannot efficiently degrade CWAs. Here, we design and prepare a hybrid membrane through in situ growth of catalytically active zirconium-based metal-organic frameworks (Zr-MOFs) on a polyelectrolyte membrane mediated by biofilm-inspired curli nanofibers (CNFs). Superior to the bare polyelectrolyte membrane, the prepared MOF-808@CNF-PQ hybrid membrane exhibits improved rejection of the nerve agent simulant dimethyl methyl phosphonate (DMMP) vapor and permeation of the water vapor by 113 and 45%, respectively. The water/DMMP selectivity of the hybrid membrane reaches 498.6, approximately 13 times that of the commercial polyelectrolyte membrane Nafion 117. In addition, the hybrid membrane possesses appreciable catalytic activity for the hydrolysis of the nerve agent simulant dimethyl 4-nitrophenyl phosphate (DMNP) with a half-life of ∼38 min. Nanomechanical characterization results based on atomic force microscopy (AFM) techniques demonstrate the critical role of CNFs in mediating Zr-MOF nucleation and the dominant effect of electrostatic interactions on self-assembly of CNFs on polyelectrolyte base. It is also confirmed that the Zr-MOF toppings serve as the key components in physically adsorbing and chemically degrading the DMNP molecules through multiple strong intermolecular interactions. Our work offers a rational strategy to develop advanced membranes toward efficient chemical protection, moisture permeation, and catalytic detoxification against CWAs.

18.
Mol Immunol ; 153: 36-41, 2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36403432

RESUMO

The innate immune system is the first line of defense for the host against any microbial attack. It can quickly identify microorganisms and produce an immune response, removing pathogenic microorganisms. However, a strong immune response might lead to excessive inflammation and even autoimmune diseases. NLRC3 is an important regulator of innate immune system homeostasis. It is a member of the anti-inflammatory NLR family and can inhibit excessive immune response in the body. In this review, we primarily focused on the current research progress on NLRC3 and its potential application. It can decrease the production of pro-inflammatory cytokines by inhibiting the NF-κB, MAK-ERK, PI3K-mTOR, IL-6/JAK2/STAT3, and cGAS-STING pathways. It also inhibits inflammatory responses by interfering with the assembly and activity of the NLRP3 inflammasome complexes. Additionally, NLRC3 can also reduce the functions of some antigen-presenting cells and their ability to activate and polarize CD4+ T cells into Th1 and Th17 subsets. NLRC3 is closely related to the development of tumors, infectious diseases, autoimmune diseases, and AD. These diseases might be treated effectively by regulating the expression of NLRC3.

19.
Chem Soc Rev ; 2022 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-36448635

RESUMO

Correction for 'Recent advances and perspectives for solar-driven water splitting using particulate photocatalysts' by Xiaoping Tao et al., Chem. Soc. Rev., 2022, 51, 3561-3608, https://doi.org/10.1039/d1cs01182k.

20.
Diabetes Metab Syndr Obes ; 15: 3397-3402, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36345491

RESUMO

Objective: To evaluate the consistency between SUDOSCAN examinations and electromyography (EMG) results in patients with diabetes. Methods: A total of 326 patients with diabetes (201 males and 125 females) who were hospitalized in the endocrinology ward of the Affiliated Hospital of Nanjing University of Chinese Medicine (Jiangsu Province Hospital of Chinese Medicine) from June 2020 to February 2021 were selected as participants. All the patients were tested using a SUDOSCAN conductance analyzer for electrical skin conductivities and EMG for nerve conduction. The differences and consistencies between the results of the two examinations were analyzed. McNemar's test was used to analyze the differences between the results, and Cohen's kappa test was utilized to test the consistencies. Results: A total of 174 patients had abnormal SUDOSCAN results, and 152 patients had normal SUDOSCAN results. The EMG results of 299 patients were abnormal, and the EMG results of 27 patients were normal. The McNemar test result was P = 0.000, and the differences between the results of the SUDOSCAN and EMG examinations were statistically significant (P < 0.01). No significant consistency was found between the SUDOSCAN and EMG results, meaning that the consistency between the two examination results was not statistically significant (P = 0.868, P > 0.05). The difference between the results was statistically significant (P < 0.05), and the consistency was poor (kappa = 0.005). Conclusion: Differences existed between the SUDOSCAN examination and EMG results, and the results of the two examination methods were inconsistent, indicating that SUDOSCAN examinations cannot replace EMG examinations for DSPN.

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