Effects of vitamin D supplementation on glucose and lipid metabolism in patients with type 2 diabetes mellitus and risk factors for insulin resistance.

BACKGROUND: Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease featured by insulin resistance (IR) and decreased insulin secretion. Currently, vitamin D deficiency is found in most patients with T2DM, but the relationship between vitamin D and IR in T2DM patients requires further investigation. AIM: To explore the risk factors of IR and the effects of vitamin D supplementation on glucose and lipid metabolism in patients with T2DM. METHODS: Clinical data of 162 T2DM patients treated in First Affiliated Hospital of Harbin Medical University between January 2019 and February 2022 were retrospectively analyzed. Based on the diagnostic criteria of IR, the patients were divided into a resistance group (n = 100) and a non-resistance group (n = 62). Subsequently, patients in the resistance group were subdivided to a conventional group (n = 44) or a joint group (n = 56) according to the treatment regimens. Logistic regression was carried out to analyze the risk factors of IR in T2DM patients. The changes in glucose and lipid metabolism indexes in T2DM patients with vitamin D deficiency were evaluated after the treatment. RESULTS: Notable differences were observed in age and body mass index (BMI) between the resistance group and the non-resistance group (both P < 0.05). The resistance group exhibited a lower 25-hydroxyvitamin D3 (25(OH)D3) level, as well as notably higher levels of 2-h postprandial blood glucose (2hPG), fasting blood glucose (FBG), and glycosylated hemoglobin (HbA1c) than the non-resistance group (all P < 0.0001). Additionally, the resistance group demonstrated a higher triglyceride (TG) level but a lower high-density lipoprotein-cholesterol (HDL-C) level than the non-resistance group (all P < 0.0001). The BMI, TG, HDL-C, 25(OH)D3, 2hPG, and HbA1c were found to be risk factors of IR. Moreover, the post-treatment changes in levels of 25(OH)D3, 2hPG, FBG and HbA1c, as well as TG, total cholesterol, and HDL-C in the joint group were more significant than those in the conventional group (all P < 0.05). CONCLUSION: Patients with IR exhibit significant abnormalities in glucose and lipid metabolism parameters compared to the non-insulin resistant group. Logistic regression analysis revealed that 25(OH)D3 is an independent risk factor influencing IR. Supplementation of vitamin D has been shown to improve glucose and lipid metabolism in patients with IR and T2DM.

Berberine induces apoptosis in non-small-cell lung cancer cells by upregulating miR-19a targeting tissue factor.

BACKGROUND: Berberine (BBR) from the widely used Chinese herbal medicine Huanglian has an array of pharmacological and biochemical properties, including anti-neoplastic activity. However, the specific mechanisms underlying these properties are unknown. The aim of this study was to explore the anti-tumor mechanisms of BBR in non-small cell lung cancer (NSCLC). METHODS: The effects of BBR on NSCLC tumor development and programmed cell death were investigated both in vivo and in vitro. Luciferase reporter assays were used to determine whether tissue factor (TF) was a target of miR-19a. RESULTS: BBR suppressed NSCLC growth and promoted apoptosis in NSCLC cells by modulating miR-19a and TF expression. Luciferase assays showed that TF was a direct inhibitory target of miR-19a in NSCLC cells. BBR induced apoptosis through the miR-19a/TF/MAPK axis. CONCLUSION: The results suggest that BBR induces apoptosis of NSCLC cells via the miR-19a/TF/MAPK signaling pathway.

Correlations of PTEN genetic polymorphisms with the risk of depression and depressive symptoms in a Chinese population.

OBJECTIVE: This study aimed to investigate the correlations of three common single nucleotide polymorphisms (SNPs) in the PTEN gene (rs701848 T>C, rs2735343 G>C and rs112025902 A>T) with the risk of depression and depressive symptoms in a Chinese population. METHODS: From July 2011 to June 2013, a total of 384 patients with depression and 400 healthy individuals were included in this study. These SNPs in the PTEN gene were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct sequencing. The Hamilton Depression Rating Scale (HAMD) was used to evaluate the severity of depression. RESULTS: The C allele of rs701848, the C allele of rs2735343 and the T allele of rs112025902 were associated with an increased risk of depression (odds ratio [OR]=3.814, 95% CI: 3.093-4.703, P<0.001; OR=2.642, 95% CI: 2.152-3.242, P<0.001; OR=2.882, 95% CI: 2.347-3.539, P<0.001; respectively). Depression patients carrying C allele (TC+CC) of rs701848 and carrying T allele (AT+TT) of rs112025902 had higher HAMD total scores and HAMD anxiety factor scores than those carrying TT genotype of rs701848 and carrying AA genotype of rs112025902 (all P<0.05). Furthermore, depression patients carrying C allele (GC+CC) of rs2735343 had lower HAMD total scores and HAMD factors associated with depression scores than those carrying GG genotype (both P<0.05). Logistic regression analysis revealed that rs701848, rs2735343 and rs112025902 polymorphisms in the PTEN gene may be independent risk factors of depression (relative risk [RR]=1.807, 95% CI=1.023-3.193, P=0.042; RR=1.759, 95% CI=1.033-2.995, P=0.038; RR=1.646, 95% CI=1.018-2.663, P=0.042; respectively). CONCLUSION: Our findings provide evidence that rs701848, rs2735343 and rs112025902 polymorphisms in the PTEN gene may be correlated with the risk of depression and depressive symptoms in the Chinese population.

Clavicle and coracoid process drilling technique for truly anatomic coracoclavicular ligament reconstruction.

BACKGROUND: High-grade acromioclavicular (AC) joint separations require surgical treatment, as conservative treatment may result in functional decline or persistent pain. Although many surgical techniques have been described in the literature, there is still no non-controversial gold standard procedure for AC joint dislocation. The different orientation of the two components of the coracoclavicular (CC) ligaments has been proven to account for different functions. However, the majority of the techniques reconstruct the CC ligaments with a single structure. The purposes of this study were to assess the feasibility of truly anatomic coracoclavicular ligament reconstruction (TACCR) and to determine the corresponding drilling parameter. METHODS: We constructed virtual three-dimensional (3D) models of 105 shoulders from computed tomography (CT) scan data by using SuperImage software. For each model, the attachment sites and footprint dimensions of the conoid and trapezoid ligaments were defined and adjusted according to previously defined anatomic parameters and individual measurement results. Virtual drilling and 3D measurement were carried out in each model separately. Guided by the drilling parameter, we performed TACCR on 24 shoulders from 12 whole cadavers after transecting the AC and CC ligaments. RESULTS: The collinear drilling technique was noted to breach the bone cortex of the clavicle and/or the coracoid process in 95 of 105 virtual models (90.5%). No cortical breach was observed using a non-collinear drilling technique. The anteversion angulation of the conoid-coracoid tunnel ranged from 10° to 15° (mean value 12.4°). The extraversion angulation of the conoid-coracoid tunnel varied from 5° to 10° (mean value 6.8°). The anteversion angulation of the trapezoid-coracoid tunnel ranged from 20° to 30° (mean value 25.2°). There were no failures when performing the non-collinear drilling technique on cadaver models. CONCLUSIONS: The collinear drilling technique is not technically feasible for TACCR. For this reason, we recommend the non-collinear drilling technique, which provides the ability to prepare bony tunnels without any risk of cortical breach. CLINICAL RELEVANCE: Based on this computer and cadaver model study, we have devised a novel drilling technique to restore the CC ligaments anatomically.

Coracoclavicular ligament attachment regions of the Chinese population: a quantitative anatomic study.

It is reported that the coracoclavicular (CC) ligaments arise at a constant region, which is proportional to the size of the clavicle and the coracoid process. However, all cadavers in those studies were from whites or African-Americans. The aim of this study was to evaluate dimension and orientation of CC footprints in Chinese cadavers and to determine whether race-dependent differences in these measurements exist. A total of 172 shoulders from 87 Chinese cadavers were used in this study, and the CC ligaments including the coracoid and the lateral clavicle were exposed. After measurement of the length of the CC ligaments, the ligaments were dissected and the insertion sites as well as the footprint centers were identified and marked. Each CC insertion dimension and its distance to the bony landmarks were recorded. Ratios representing the distance from the clavicular landmarks to each footprint center divided by clavicular length and clavicular width were calculated. These ratios were calculated for the coracoid process as well. The mean length of the clavicle and the coracoid process was 139.9 ± 9.4 and 40.5 ± 4.0 mm. The distance from the lateral edge of the clavicle to the conoidal center and to the trapezoidal center was 35.7 ± 3.4 and 21.8 ± 2.7 mm, respectively. The distance from the tip of the coracoid to the conoidal center and to the trapezoidal center was 35.1 ± 3.2 and 29.7 ± 2.9 mm, respectively. The ratios of the distance to the conoidal center and to the trapezoidal center divided by clavicular length and coracoidal length were 25.5, 15.6, 86.8 and 73.4 %, respectively. While absolute differences in the origin of the CC ligaments exist between different races, the ratio of these origins to the size of the clavicle and the coracoid process is constant.

[Investigation of reproductive organs of male children and juvenile of the Meng and the Han nationality in Chifeng area].

OBJECTIVES: To investigate the development and the health of reproductive organs of male children and juvenile between the Meng and the Han nationality in the Meng nationality area. METHODS: Male juvenile(4-18 years old) of the Meng nationality (n = 2,315) and the Han nationality (n = 2,832) were divided into four age groups. Height, weight, length and perimeter of penis, volume of left and right testis and reproductive organs illness were examined. RESULTS: In 13-18 years group, the developmental speed of reproductive organs was faster in Mongolia male juvenile than that in the Han nationality (P < 0.02). After 13 years old, the developmental speed of reproductive organs of male living in town is faster than that in the country (P < 0.05). Illness of male reproductive organs was common such as hernia, varicocle etc. CONCLUSIONS: There was difference of developmental status and the prevalence rate of reproductive organs of male children and juvenile between the Meng and the Han nationality.