RESUMO
The objective of the present study was to evaluate the behavior of piglets in the nursery phase in the presence of pure sisal ropes, soaked with different flavors as a means of environmental enrichment. Sixty-four piglets with 35 days of age in the nursery phase were used, piglets were randomly distributed in a completely randomized design in 16 pens, with four animals in each pen and there was no separation of pens by group. Three treatments were used: Pure rope (PR) which was considered the control of the pen, rope soaked in vanilla essence and sugar (SV), and rope soaked in coffee (SC). An ethogram was prepared for observations of the following behavioral parameters, eating, drinking, urinating, defecating, lying down, standing, playful, and others. The parameters were measured every 10 minutes in a total of three daily assessments with two hours each for a period of three days. There was a greater preference of piglets for the SV rope with a percentage of 63.2% in the observations of the interactions. A possible justification for the result is the fact that this rope is the most palatable for piglets since piglets prefer sweet tastes. The PR rope aroused curiosity in the piglets, with a percentage of observations of 21.4%. Therefore, the use of equipment flavored with sweet vanilla taste is promising as a means of environmental enrichment, being more interesting to piglets.
Assuntos
Animais , Comportamento Animal/efeitos dos fármacos , Suínos , Vanilla/efeitos adversosRESUMO
Background Aspergillus niger has the ability to secrete feruloyl esterase. However, for economically viable industrial applications, it is necessary to increase their catalytic activities and/or protein yields to satisfy the increasing needs for feruloyl esterases. Results The gene AnFaeA that encodes a type A feruloyl esterase was successfully expressed in Pichia pastoris by a two-copy engineered yeast. After a screen in shaker flask, a one-copy strain GSKFA3 having the highest feruloyl esterase activity of 2.4 U/mL was obtained. Then, the pPICZaA-AnFaeA plasmid was transformed into GSKFA3 and the transformants were grown on YPDS plates with antibiotic Zeocin. After cultivation, a two-copy strain GSKZaFA20 with the highest feruloyl esterase activity of 15.49 U/mL was obtained. The expressed protein (recombinant AnFaeA) may be a glycoprotein with an apparent molecular weight of 40 kDa. It displayed the maximum activity at pH 6.0 and 50°C, and was stable at a pH range of 4.0-6.5 and at below 45°C. Its activity was not significantly affected by K+, Ca2 +, Mg2 +, Cu2 +, Zn2 +, Mn2 +, Na+ and EDTA, but activated by Fe2 +. The Km and Vmax toward 4-nitrophenyl ferulate were 5.5 mM and 69.0 U/mg, respectively. Conclusions The two-copy strain GSKZaFA20 showed a 4.4-fold increase in extracellular enzyme activity compared with the one-copy strain GSKFA3. Construction of two-copy strain improved secretion of recombinant AnFaeA in P. pastoris.
Assuntos
Pichia/enzimologia , Aspergillus niger/enzimologia , Hidrolases de Éster Carboxílico/metabolismo , Leveduras , Western BlottingRESUMO
Complementary near-infrared (NIR) and Raman mapping techniques have been used to study the distribution of drug particles suspended in a polymeric film. The film configuration was used with the goal of maintaining a drug with a particle size of less than 10 µm in a nonagglomerated form and to satisfy two commonly encountered pharmaceutical needs: enhanced dissolution rate of poorly soluble drugs and the content uniformity of drugs administered in low doses. A total of four film batches were prepared for this study using hydroxypropyl methylcellulose (HPMC) with griseofulvin as the active pharmaceutical ingredient. The NIR method analyzed a film area of 3 × 2.6 mm, whereas Raman mapping analyzed an area of 10 × 10 µm, and every sample was analyzed by the two methods. The second derivative transform removed baseline variations in the NIR spectra and provided differentiation between the two components. NIR chemical imaging did not identify clusters larger than 0.05 mm. Raman analysis identified areas rich in griseofulvin or HPMC, which were used to develop a partial least squares discriminant analysis method to determine drug or polymer distribution throughout the film.