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1.
J Vis Exp ; (163)2020 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-33044457

RESUMO

Membrane proteins play essential roles in a variety of cellular processes and perform vital functions. Membrane proteins are medically important in drug discovery because they are the targets of more than half of all drugs. An obstacle to conducting biochemical, biophysical, and structural studies of membrane proteins as well as antibody development has been the difficulty in producing large amounts of high-quality membrane protein with correct conformation and activity. Here we describe a "bilayer-dialysis method" using a wheat germ cell-free system, liposomes, and dialysis cups to efficiently synthesize membrane proteins and prepare purified proteoliposomes in a short time with a high success rate. Membrane proteins can be produced as much as in several milligrams, such as GPCRs, ion channels, transporters, and tetraspanins. This cell-free method contributes to reducing the time, cost and effort for preparing high-quality proteoliposomes, and provides suitable means for functional analysis of membrane proteins, drug targets screening, and antibody development.

2.
Appl Radiat Isot ; 167: 109471, 2020 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-33068986

RESUMO

The CR-(RC)n filter is used for Semi-Gaussian pulse shaping in analog spectroscopy. A new digital CR-(RC)n filter is presented based on the recursive models of the CR filter and RC filter. The spectroscopic signal reconstruction technique is proposed. This technique is applied to digital exponential decay signals obtained by the digitization of analog signals that represent the combined response of a FAST-SDD detector and the associated front-end circuit. Compared with the direct application of the digital CR-(RC)n filter, the technique eliminates the undershoot of the Semi-Gaussian pulse which is the primary cause of the low-energy peak tailing. And, the digital pole-zero cancellation filter follows digital CR-(RC)n filter to reduce the long tail of the shaped Semi-Gaussian pulse which leads to the high-energy peak tailing. Experimental results based on XRF signals of manganese sample show that the proposed techniques can cancel peak tailing due to the shaped Semi-Gaussian pulse improving FWTM/FWHM ratio and throughput capability.

3.
Oncogene ; 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33082557

RESUMO

As a result of the hostile microenvironment, metabolic alterations are required to enable the malignant growth of cancer cells. To understand metabolic reprogramming during metastasis, we conducted shotgun proteomic analysis of highly metastatic (HM) and non-metastatic (NM) ovarian cancer cells. The results suggest that the genes involved in fatty-acid (FA) metabolism are upregulated, with consequent increases of phospholipids with relatively short FA chains (myristic acid, MA) in HM cells. Among the upregulated proteins, ACSL1 expression could convert the lipid profile of NM cells to that similar of HM cells and make them highly aggressive. Importantly, we demonstrated that ACSL1 activates the AMP-activated protein kinase and Src pathways via protein myristoylation and finally enhances FA beta oxidation. Patient samples and tissue microarray data also suggested that omentum metastatic tumours have higher ACSL1 expression than primary tumours and a strong association with poor clinical outcome. Overall, our data reveal that ACSL1 enhances cancer metastasis by regulating FA metabolism and myristoylation.

4.
Environ Pollut ; 268(Pt B): 115004, 2020 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-33010674

RESUMO

Nitrate leaching caused by overusing or misusing nitrogen (N) fertilizers in field vegetable cropping systems in China is a leading contributor to nitrate contamination of groundwater. Identification of the critical fertilizer N input rate could support management decisions that maintain yields while reducing the impact of nitrate leaching on groundwater. A four-season field experiment involving six N treatments (0, 60, 120, 180, 240, and 300 kg N ha-1) was undertaken to investigate the impacts of various N rates on N use efficiency (NUE), seasonal nitrate leaching loss (SNLL), nitrate residue (NR), and radish yield, and to identify the critical N fertilizer rate for both optimum yield and minimum nitrate leaching loss in a field vegetable (radish, Raphanus sativus L.) cropping system in northern China. The results showed that radish yield enhanced quadratically and NUE reduced linearly with increasing N addition, while the NR and SNLL increased exponentially. The yield did not increase markedly when N fertilization exceeded 180 kg N ha-1. SNLL and nitrate concentrations in the leachate averaged 11.5-71.5 kg N ha-1 and 5.1-35.6 mg N L-1, respectively, under N rates of 60-300 kg N ha-1. The results showed that N fertilizer rate ranging from 180 to 196 kg N ha-1 resulted in high yields and low nitrate leaching losses. Compared with those in response to the N fertilizer amount applied by local farmers, the NUE, NR, and SNLL in response to the N fertilizer amount identified in this study increased, decreased by 30.9%-35.0%, and decreased by 49.9%-55.7%, respectively, without any yield loss. Thus, a critical N fertilizer rate ranging from 180 to 196 kg N ha-1 is recommended to obtain optimum yields with minimal environmental risks in radish fields in northern China.

5.
Chem Biol Interact ; 332: 109285, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33038330

RESUMO

Ginsenoside Rb1 (GsRb1) is the best constituent of ginseng and although it shows clinical efficacy as an antineoplastic, antioxidative and antirheumatic agent, its oral bioavailability is poor due to its limited solubility. In this study, the solubility of GsRb1 was improved by encapsulating it in polymeric nanocapsules (encapsulation efficiency: 99.79%), therefore, improving the oral bioavailability. The encapsulation resulted in stable, homogenous and well-dispersed nano-GsRb1, whose mean particle size and zeta potential were 183.9 nm and +36.9 mV, respectively. A significant improvement was observed in the in vitro release profile of nano-GsRb1 as compared to its free form. Our study also indicated a significant repression of the degradation of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα), the nuclear factor kappa B (NF-κB) signaling pathway, NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome activation, and the mitochondrial damage, thereby, reducing inflammation and gouty arthritis induced by monosodium urate (MSU), when compared to free GsRb1, strongly suggesting that polymeric nano-particles can be a novel approach for delivering the GsRb1 into the inflamed joints for a better treatment effectiveness.

6.
Nanoscale Horiz ; 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33063803

RESUMO

Compared with the highly active anatase TiO2, rutile TiO2 usually presents poor photocatalytic performance due to high electron-hole recombination. Herein, we propose a surface domain heterojunction (SDH) structure between adjacent micro-domains with and without chemisorbed chlorine on rutile TiO2, which utilizes the potential difference between these domains to form a built-in field that promotes charge separation. Single-crystal rutile TiO2 nanorods assembled into radial microspheres with SDHs were fabricated, and these exhibited excellent solar-driven photocatalytic hydrogen evolution, ∼8-fold higher than that of the pristine one. Experimental results and density functional theory calculations reveal that the exceptional photocatalytic performance can be attributed to the in situ formation of chemisorbed chlorine, which forms SDHs that separate electrons and holes efficiently and results in surface reconfiguration, exposing the tri-active sites, increasing the O-site active centers and enhancing the catalytic activity of the 4-coordinated (Ti4c) and 5-coordinated Ti sites (Ti5c). This SDH strategy can extend to other halogen elements and thus provides an universal approach for the rational design of high-efficiency TiO2 photocatalysts toward sustainable solar-fuel evolution.

7.
Phys Med Biol ; 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33065559

RESUMO

The clinical demand for low image noise often limits the slice thickness used in many CT applications. However, a thick-slice image is more susceptible to longitudinal partial volume effects, which can blur key anatomic structures and pathologies of interest. In this work, we develop a prior-knowledge-aware iterative denoising (PKAID) framework that utilizes spatial data redundancy in the slice increment direction to generate low-noise, thin-slice images, and demonstrate its application in non-contrast head CT exams. The proposed technique takes advantage of the low-noise of thicker images and exploits the structural similarity between the thick- and thin-slice images to reduce noise in the thin-slice image. Phantom data and patient cases (n=3) of head CT were used to assess performance of this method. Images were reconstructed at clinically-utilized slice thickness (5 mm) and thinner slice thickness (2 mm). PKAID was used to reduce image noise in 2 mm images using the 5 mm images as low-noise prior. Noise amplitude, noise power spectra (NPS), modulation transfer function (MTF), and slice sensitivity profiles (SSP) of images before/after denoising were analyzed. The NPS and MTF analysis showed that PKAID preserved noise texture and resolution of the original thin-slice image, while reducing noise to the level of thick-slice image. The SSP analysis showed that the slice thickness of the original thin-slice image was retained. Patient examples demonstrated that PKAID-processed, thin-slice images better delineated brain structures and key pathologies such as subdural hematoma compared to the clinical 5 mm images, while additionally reducing image noise. To test an alternative PKAID utilization for dose reduction, a head exam with 40% dose reduction was simulated using projection-domain noise insertion. The image of 5 mm slice thickness was then denoised using PKAID. The results showed that the PKAID-processed reduced-dose images maintained similar noise and image quality compared to the full-dose images.

8.
Qual Life Res ; 2020 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-33068235

RESUMO

PURPOSE: At present, it is not clear about the influence of health-promoting lifestyle, aging perceptions, social support, and other psychosocial factors on elderly depression. This study aims to explore the mediating role of aging perceptions between health-promoting lifestyle and elderly depression, and the moderating role of social support in the mediating process. METHODS: A cross-sectional survey was conducted among 359 elderly people in six districts of a city. The Chinese version of the Health-Promoting Lifestyle Profile-II (HPLP-IIR), the Brief Aging Perceptions Questionnaire (B-PQ), the Center for Epidemiological Studies Depression Scale (CES-DR), and the Social Support Rate Scale (SSRS) were conducted and recollected on the spot. Stepwise analysis was used to test the mediating effect and moderating effect, and age and gender variables were controlled. RESULTS: The results showed the following: (1) health-promoting lifestyle is an important influencing factor of elderly depression; (2) aging perceptions plays a mediating role in the relationship between health-promoting lifestyle and elderly depression, accounting for 31.8% of the total utility; and (3) social support plays a moderating role between aging perceptions and elderly depression, with a high level of social support. The effect of aging perceptions on depression is less than that of the elderly with low social support level. CONCLUSION: Health-promoting lifestyle influence the depression of elderly people through aging perceptions and social support moderates the influence of aging perceptions on the elderly depression.

9.
Artigo em Inglês | MEDLINE | ID: mdl-33017285

RESUMO

Autophagy, or cellular self-digestion, is an essential process for eliminating abnormal protein in mammalian cells. Accumulating evidence indicates that increased neuronal autophagy has a protective effect on neurodegenerative disorders. It has been reported that low-intensity pulsed ultrasound (LIPUS) can non-invasively modulate neural activity in the brain. Yet, the effect of LIPUS on neuronal autophagy is still unclear. The objective of this study was to examine whether LIPUS stimulation could induce neuronal autophagy. Primary neurons were treated by LIPUS with frequency of 0.68 MHz, pulse repetition frequency (PRF) of 500 Hz, spatial peak temporal-average intensities (ISPTA) of 70 and 165 mW/cm2. Then immunofluorescent analysis of LC3B was carried out for evaluating neuronal autophagy. Further, 0.5 MHz LIPUS was non-invasively delivered to the cortex and hippocampus of adult mice (n = 16) with PRF of 500 Hz and ISPTA of 235 mW/cm2. The LC3BII/LC3BI ratio and p62 (autophagic markers) were measured by western blot analysis. In in vitro study, the expression of LC3B in primary neurons was statistically improved after LIPUS stimulation was implemented for 4 hours (p < 0.01). With the increase of irradiation duration or acoustic intensity of LIPUS stimulation, the expression of LC3B in primary neurons was increased. Furthermore, transcranial LIPUS stimulation increased the LC3BII/LC3BI ratio (p < 0.05), and decreased the expression of p62 (p < 0.05) in the cortex and hippocampus. We concluded that LIPUS provides a safe and capable tool for activating neuronal autophagy in vitro and in vivo.

10.
Sci Rep ; 10(1): 16998, 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33046785

RESUMO

Most initially perfect flowers of Toona ciliata Roem subsequently develop into functionally unisexual flowers and their relative positions in the same inflorescence could enhance the outcrossing system in this species. Here we investigated the mating system of this species. We used eight nuclear microsatellite markers and investigated the progeny of 125 mother trees from six populations naturally distributed in South China, with sample sizes ranging from 64 to 300 seeds. The multilocus outcrossing rate was 0.970 ± 0.063, and the single locus outcrossing rate was 0.859 ± 0.106, indicating the pattern of predominant outcrossing. Selfing was present in one population, but biparental inbreeding occurred in five populations. Inbreeding was absent in maternal parents, and correlations of selfing among families or among loci were generally insignificant. Positive correlation of paternity at multiple loci was significant in four populations, but was not consistent with the results at single loci. Population substructure occurred in male similarity between outcrosses only in one population. Population genetic differentaitaion was significant (Fst = 34.5%) and the effects of isolation-by-distance at the eight loci were significant among the six populations. These results provide evidence that self-comptability and inbreeding naturally occur in T. ciliata and indicate that inbreeding avoidance is necessary during genetic improvement and breeding of this endangered tree species.

11.
Sci Rep ; 10(1): 17617, 2020 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-33077783

RESUMO

Heterologous expression is the main approach for recombinant protein production ingenetic synthesis, for which codon optimization is necessary. The existing optimization methods are based on biological indexes. In this paper, we propose a novel codon optimization method based on deep learning. First, we introduce the concept of codon boxes, via which DNA sequences can be recoded into codon box sequences while ignoring the order of bases. Then, the problem of codon optimization can be converted to sequence annotation of corresponding amino acids with codon boxes. The codon optimization models for Escherichia Coli were trained by the Bidirectional Long-Short-Term Memory Conditional Random Field. Theoretically, deep learning is a good method to obtain the distribution characteristics of DNA. In addition to the comparison of the codon adaptation index, protein expression experiments for plasmodium falciparum candidate vaccine and polymerase acidic protein were implemented for comparison with the original sequences and the optimized sequences from Genewiz and ThermoFisher. The results show that our method for enhancing protein expression is efficient and competitive.

12.
Tree Physiol ; 2020 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-33079182

RESUMO

Conspicuous color is a common trait of foliar galls, but their relationship with gall-inducing insects is unknown. Red and green galls were taken from sunny or shady parts of peach species Prunus persica f. rubro-plena with peach aphid Tuberocephalus momonis infestation. We found that the loss of photosynthetic pigments was associated with the conspicuous coloration of green gall tissues. The concentrations of anthocyanins significantly increased following UV irradiation of green gall tissues, suggesting that accumulation of anthocyanins in red galls is related to UVB and UVC radiation. The expression of structural genes related to the biosynthesis of chlorogenic acid and malic acid benzoate were increased in all gall tissues and negatively correlated with the expression profiles of certain genes associated with photosynthetic biosynthesis, indicating that the increased transcript levels of the phenylpropanoid pathway might cause loss of photosynthetic efficiency in the gall tissues. Transcriptome and qRT-PCR analyses revealed that MYB transcription factors that up-regulate the biosynthesis of anthocyanins in red gall tissues might be activated by both UVB and UVC exposure. Comet assays suggest that green and red gall tissues have similar DNA damage following UV irradiation. No obvious effect of the up-regulated compounds on the growth of the peach aphid was observed. Interestingly, peach aphids under leaves painted with anthocyanins had lower mortality following UV irradiation than those in controls. These results suggest that the anthocyanins in red gall tissues have a defensive function for the peach aphid, protecting it against UV radiation.

13.
Sci Rep ; 10(1): 17166, 2020 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-33051568

RESUMO

Evidence regarding the association between blood lead levels (BLL) and hyperhomocysteinemia (HHcy) in US adults was limited. We aimed to investigate the association of BLL with the risk of HHcy, and to examine possible effect modifiers using US National Health and Nutrition Examination Survey (NHANES) database. We performed a cross-sectional study using data from up to 9,331 participants aged ≥ 20 years of NHANES from 2001 to 2006. BLL was measured by atomic absorption spectrometry. HHcy was defined as plasma homocysteine level > 15 µmol/L. The weighted prevalence of HHcy was 6.87%. The overall mean BLL was 1.9 µg/dL. Overall, there was a nonlinear positive association between Ln-transformed BLL (LnBLL) and the risk of HHcy. The Odds ratios (95% CI) for participants in the second (0.04-0.49 µg/dL), third (0.5-0.95 µg/dL) and fourth quartiles (> 0.95 µg/dL) were 1.12 (95% CI: 0.71, 1.76), 1.13 (95% CI: 0.73, 1.77), and 1.67 (95% CI: 1.07, 2.61), respectively, compared with those in quartile 1. Consistently, a significantly higher risk of HHcy (OR: 1.49; 95% CI: 1.19, 1.88) was found in participants in quartile 4 compared with those in quartiles 1-3. Furthermore, a strongly positive association between LnBLL and HHcy was observed in participants with estimated glomerular filtration rate (eGFR) < 60 mL/min-1/1.73 m-2. Our results suggested that a higher level of BLL (LnBLL > 0.95 µg/dL) was associated with increased risk of HHcy compared with a lower level of BLL (LnBLL ≤ 0.95 µg/dL) among U.S. adults, and the association was modified by the eGFR.

14.
J Diabetes Res ; 2020: 3634974, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33015191

RESUMO

Tangshen Formula (TSF) is a Chinese Medicine formula that has been reported to alleviate proteinuria and protect renal function in humans and animals with diabetic kidney disease (DKD). However, little is known about its mechanism in improving proteinuria. The dysregulation of podocyte cell-matrix adhesion has been demonstrated to play an important role in the pathogenesis and progression of proteinuric kidney diseases including DKD. In the present study, the underlying protective mechanism of TSF on podocytes was investigated using the murine model of type 2 DKD db/db mice in vivo and advanced glycation end products (AGEs)-stimulated primary mice podocytes in vitro. Results revealed that TSF treatment could significantly mitigate reduction of podocyte numbers and foot process effacement, reduce proteinuria, and protect renal function in db/db mice. There was a significant increase in expression of transient receptor potential canonical channel 6 (TRPC6) and a decrease in expression of talin1 in podocytes of db/db mice. The results of AGEs-stimulated primary mice podocytes showed increased cell migration and actin-cytoskeleton rearrangement. Moreover, primary mice podocytes stimulated by AGEs displayed an increase in TRPC6-dependent Ca2+ influx, a loss of talin1, and translocation of nuclear factor of activated T cell (NFATC) 2. These dysregulations in mice primary podocytes stimulated by AGEs could be significantly attenuated after TSF treatment. 1-Oleoyl-2-acetyl-sn-glycerol (OAG), a TRPC6 agonist, blocked the protective role of TSF on podocyte cell-matrix adherence. In conclusion, TSF could protect podocytes from injury and reduce proteinuria in DKD, which may be mediated by the regulation of the TRPC6/Talin1 pathway in podocytes.

15.
Med Phys ; 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33020942

RESUMO

PURPOSE: To develop a convolutional neural network (CNN) that can directly estimate material density distribution from multi-energy CT images without performing conventional material decomposition. METHODS: The proposed CNN (denoted as Incept-net) followed the general framework of encoder-decoder network, with an assumption that local image information was sufficient for modeling the non-linear physical process of multi-energy CT. Incept-net was implemented with a customized loss function, including an in-house-designed image-gradient-correlation (IGC) regularizer to improve edge preservation. The network consisted of two types of customized multi-branch modules exploiting multi-scale feature representation to improve the robustness over local image noise and artifacts. Inserts with various densities of different materials (hydroxyapatite (HA), iodine, a blood-iodine mixture, and fat) were scanned using a research photon-counting-detector (PCD) CT with two energy thresholds and multiple radiation dose levels. The network was trained using phantom image patches only, and tested with different-configurations of full field-of-view phantom and in vivo porcine images. Further, the nominal mass densities of insert materials were used as the labels in CNN training, which potentially provided an implicit mass conservation constraint. The Incept-net performance was evaluated in terms of image noise, detail preservation, and quantitative accuracy. Its performance was also compared to common material decomposition algorithms including least-square-based material decomposition (LS-MD), total-variation regularized material decomposition (TV-MD), and a U-net based method. RESULTS: Incept-net improved accuracy of the predicted mass density of basis materials compared with the U-net, TV-MD, and LS-MD: the mean absolute error (MAE) of iodine was 0.66, 1.0, 1.33, and 1.57 mgI/cc for Incept-net, U-net, TV-MD and LS-MD, respectively, across all iodine-present inserts (2.0 to 24.0 mgI/cc). With the LS-MD as the baseline, Incept-net and U-net achieved comparable noise reduction (both around 95%), both higher than TV-MD (85%). The proposed IGC regularizer effectively helped both Incept-net and U-net to reduce image artifact. Incept-net closely conserved the total mass densities (i.e. mass conservation constraint) in porcine images, which heuristically validated the quantitative accuracy of its outputs in anatomical background. In general, Incept-net performance was less dependent on radiation dose levels than the two conventional methods; with approximately 40% less parameters, the Incept-net achieved relatively improved performance than the comparator U-net, indicating that performance gain by Incept-net was not achieved by simply increasing network learning capacity. CONCLUSION: Incept-net demonstrated superior qualitative image appearance, quantitative accuracy, and lower noise than the conventional methods and less sensitive to dose change. Incept-net generalized and performed well with unseen image structures and different material mass densities. This study provided preliminary evidence that the proposed CNN may be used to improve the material decomposition quality in multi-energy CT.

16.
Chem Commun (Camb) ; 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33001080

RESUMO

A novel expanded metal-organic framework (UTSA-111a) with functional pyrimidine sites exhibits simultaneously high gravimetric and volumetric methane storage working capacities of 309 cm3 (STP) g-1 and 183 cm3 (STP) cm-3 at 298 K and 5.8-65 bar.

17.
Nanoscale ; 2020 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-33030190

RESUMO

This study focused on the processing and photothermal healing of gold nanoparticle (Au NP) and polystyrene (PS) hybrid films. Effects of Au NP contents were investigated using hybrid films with the NP content from 0 to 1 wt% via a solvent-assisted approach. The as-synthesized Au NPs showed an average diameter of 4-5 nm with a face-centered cubic structure. The Au NP agglomeration deteriorated as the content increased and the interparticle distance decreased. The film transparency and flexibility also decreased with the NP content. The Au-PS films demonstrated desirable photothermal healing behaviors, which required more energy with the defect size increase. The simulated temperature distribution on the hybrid films during the photo-induced healing showed good agreement with the experimental results, with particle agglomeration degrading the healing properties. The developed hybrid films can be used in functional devices and coatings with high flexibility and healed using photon energy sources.

18.
J Cardiothorac Surg ; 15(1): 302, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33028372

RESUMO

OBJECTIVES: This study aimed to identify the incidence rate of Acute kidney injury (AKI) in our center and predict in-hospital mortality and long-term survival after heart transplantation (HTx). METHODS: This single-center, retrospective study from October 2009 and March 2020 analyzed the pre-, intra-, and postoperative characteristics of 95 patients who underwent HTx. AKI was defined according to the Kidney Disease: Improving Global Outcomes (KDIGO) criteria. Risk factors were analyzed by multivariable logistic regression models. The log-rank test was used to compare long-term survival. RESULTS: Thirty-three (34.7%) patients developed AKI. The mortality in hospital in HTx patients with and without AKI were 21.21 and 6.45%, respectively (P < 0.05). Recipients in AKI who required renal replacement therapy (RRT) had a hospital mortality rate of 43.75% compared to 6.45% in those without AKI or RRT (P < 0.0001). A long cardiopulmonary bypass (CPB) time (OR:11.393, 95% CI: 2.183 to 59.465, P = 0.0039) was positively related to the occurrence of AKI. A high intraoperative urine volume (OR: 0.031, 95% CI: 0.005 to 0.212, P = 0.0004) was negatively correlated with AKI. AKI requiring RRT (OR, 11.348; 95% CI, 2.418-53.267, P = 0.002) was a risk factor for mortality in hospital. Overall survival in patients without AKI at 1 and 3 years was not different from that in patients with AKI (P = 0.096). CONCLUSIONS: AKI is common after HTx. AKI requiring RRT could contribute powerful prognostic information to predict mortality in hospital. A long CPB time and low intraoperative urine volume are associated with the occurrence of AKI.

19.
Artigo em Inglês | MEDLINE | ID: mdl-33035388

RESUMO

The authors aimed to evaluate the relationship of serum albumin with peripheral arterial disease (PAD) and investigate any possible effect modifiers in hypertensive patients. In the cross-sectional study, a total of 10,900 Chinese hypertensive patients aged ≥18 years were enrolled. The outcome was PAD, defined as an ankle-brachial index (ABI) <0.90 in either leg. The overall mean (SD) serum albumin was 46.8 (4.2) g/L. There were significant inverse associations of serum albumin with PAD (per SD increment; OR: 0.83; 95% CI: 0.72, 0.94). Compared with the lowest tertile (<45.1 g/L), the multivariate-adjusted ORs (95% CI) for participants in the middle tertile (45.1-48.2 g/L) and highest tertile (≥48.2 g/L) of serum albumin were 0.89 (95% CI: 0.67, 1.16) and 0.65 (95% CI: 0.47, 0.90), respectively. Conversely, lower albumin (<48.2 g/L) concentrations were associated with increased odds of PAD (OR: 1.45; 95% CI: 1.08-1.96) compared with higher concentrations. Furthermore, the albumin-PAD association was significantly stronger in males (OR: 2.08; 95% CI: 1.31, 3.30) than in females (OR: 1.06; 95% CI: 0.70, 1.58; p interaction = .024). In conclusion, among Chinese hypertensive adults, lower serum albumin was associated with the prevalence of PAD only in males but not in females.

20.
Biochim Biophys Acta Rev Cancer ; 1874(2): 188443, 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33035642

RESUMO

Fibroblasts have gained increasing attention in tissue desmoplasia, especially in tumors. Activated fibroblasts from various sources modulate matrix composition and stiffness associated with organ fibrosis as well as tumor progression. More importantly, cancer-associated fibroblasts (CAFs) interacts with both cancer cells and other stromal cells, providing a tumor-promoting microenvironment for tumor invasion and metastasis. However, CAF biology is not fully understood due to its heterogeneity. Here, we describe the main transforming cues that contribute to CAF activation and unveil the expanding CAF heterogeneity associated with tumor progression in multiple aspects. Finally, we summarize the prospective promising and challenging stroma-targeted anti-tumor strategies.

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