Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.083
Filtrar
1.
Chemosphere ; 270: 129541, 2021 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-33429234

RESUMO

Several technologies have been employed to treat greywater (GW) for domestic use. Aerobic biological treatment has achieved high efficiency, the main cost being the necessary source of oxygen (O2). This study explores the effects of lumen air pressure (LAP) on reactor performance and microbial community succession in an O2-based membrane biofilm reactor (O2-MBfR) treating GW. At high LAP (≥0.8 psi), the dissolved oxygen (DO) concentration inside the reactor was higher than 0.38 ± 0.02 mg/L, leading to removal efficiencies of 90%, 98%, and 80%, of total chemical oxygen demand, total linear alkylbenzene sulfonate (LAS), and total nitrogen, respectively. Lower LAP (<0.8 psi) led to a decrease in DO inside the system, and a less effective GW treatment. Low O2 pressure decreased organic biodegradation and ammoniation, and caused LAS accumulation in the biofilm, leading to the solubilization of extracellular polymeric substances and cell lysis. Comprehensive consideration of reactor performance and energy input, DO inside the MBfR at 0.38 ± 0.02 mg/L could be selected as the optimized condition for GW treatment. Microbial community analyses results also revealed that improved LAP was favorable for the enrichment of LAS-biodegradation related genus (Pseudomonas, Parvibaculum, Magnetospirillum, Clostridium, Zoogloea, Dechloromonas and Mycobacterium), nitrifiers (Nitrosomonas and Sphingomonas) and facultative microorganisms (Dechloromonas, Flavobacterium, Pseudomonas, Aeromonas and Zoogloea) that can carry out denitrification under relatively high DO conditions (>0.38 mg/L), but led to the reduction of the relative abundance of heterotrophs (Acidovorax, Thermomonas, Brevundimonas and Enterobacter) that are more sensitive towards high DO conditions.

2.
Am J Prev Med ; 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33485723

RESUMO

INTRODUCTION: Trends in blood lead levels in the same birth cohort (generation) are necessary to identify the lead load in the population. This analysis uses a nationally representative sample to investigate the trends in blood lead levels from 1999 to 2016 by birth cohort and to revisit the association between blood lead levels and age. METHODS: Data from the 1996 to 2016 National Health and Nutrition Examination Surveys were used to describe the distribution of blood lead levels. Trends in blood lead levels were analyzed using joinpoint regression models. Association of blood lead levels with age was conducted with both cross-sectional and birth cohort analysis. Analyses were conducted in 2020. RESULTS: In total, 68,877 participants were included (weighted mean age=38.4 years, 50.6% female). From 1999 to 2016, the geometric mean of blood lead levels decreased from 1.68 µg/dL (95% CI=1.63, 1.74) to 0.82 µg/dL (95% CI=0.77, 0.87). The annual percentage change estimated by the joinpoint model was -4.26% (p<0.05). The associations between blood lead levels and age were "U"-shaped by cross-sectional analysis, with higher risks for the lowest and highest ages. However, by birth cohort analysis the blood lead levels declined monotonically with age. The joinpoint analysis indicated the inflection point of age 13-17 years and statistically significant differences in decline slopes before and after this age. CONCLUSIONS: In this nationally representative study of the U.S. population, estimates of blood lead levels showed an overall decrease from 1999 to 2016. Blood lead levels are highest in childhood.

3.
J Am Coll Cardiol ; 77(3): 271-281, 2021 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-33478650

RESUMO

BACKGROUND: Short-term exposure to ambient air pollution has been linked to occurrence of myocardial infarction (MI); however, only a limited number of studies investigated its association with death from MI, and the results remain inconsistent. OBJECTIVES: This study sought to investigate the association of short-term exposure to air pollution across a wide range of concentrations with MI mortality. METHODS: A time-stratified case-crossover study was conducted to investigate 151,608 MI death cases in Hubei province (China) from 2013 to 2018. Based on each case's home address, exposure to particulate matter with an aerodynamic diameter ≤2.5 µm (PM2.5), particulate matter with an aerodynamic diameter ≤10 µm (PM10), sulfur dioxide, nitrogen dioxide (NO2), carbon monoxide, and ozone on each of the case and control days was assessed as the inverse distance-weighted average concentration at neighboring air quality monitoring stations. Conditional logistic regression models were implemented to quantify exposure-response associations. RESULTS: Exposure to PM2.5, PM10, and NO2 (mean exposure on the same day of death and 1 day prior) was significantly associated with increased odds of MI mortality. The odds associated with PM2.5 and PM10 exposures increased steeply before a breakpoint (PM2.5, 33.3 µg/m3; PM10, 57.3 µg/m3) and flattened out at higher exposure levels, while the association for NO2 exposure was almost linear. Each 10-µg/m3 increase in exposure to PM2.5 (<33.3 µg/m3), PM10 (<57.3 µg/m3), and NO2 was significantly associated with a 4.14% (95% confidence interval [CI]: 1.25% to 7.12%), 2.67% (95% CI: 0.80% to 4.57%), and 1.46% (95% CI: 0.76% to 2.17%) increase in odds of MI mortality, respectively. The association between NO2 exposure and MI mortality was significantly stronger in older adults. CONCLUSIONS: Short-term exposure to PM2.5, PM10, and NO2 was associated with increased risk of MI mortality.

4.
Environ Res ; : 110735, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33460631

RESUMO

The large amount of missing values has challenged the application of satellite-retrieved aerosol optical depth (AOD) in mapping surface PM2.5 concentrations. In this study, we developed a non-AOD random forest model to estimate daily concentrations of PM2.5 in Guangdong Province, China, where more than 80% of AOD data were missing. The predictive ability of the non-AOD model was compared with that of AOD-based model. Daily ground-based measurements of PM2.5 were obtained from 148 ground-monitoring sites in Guangdong with a 60 km rectangle buffer from January 2016 to December 2018. Daily MODIS MAIAC AOD were downloaded from NASA at a resolution of approximately 1 km. Two random forest models were developed to predict ground-level PM2.5 with one included AOD as a predictor and the other one without AOD. The two random forest models were developed using the same dataset and their predictive abilities were compared. The results for 10-fold cross validation show that the non-AOD and AOD-based random forest models showed similar model performance. The R2 (RMSE) for the non-AOD model in 2016-2018 were 0.82 (8.4 µg/m3), 0.81 (9.5 µg/m3) and 0.78 (9.4 µg/m3), and those for AOD-based model were 0.83 (8.2 µg/m3), 0.82 (9.2 µg/m3) and 0.80 (9.0 µg/m3). Higher consistency of estimated PM2.5 were observed in areas close to monitoring sites than those far away from sites, and in southern coastal than northern areas. The non-AOD random forest model showed similar performance as the AOD-based model. As the non-AOD random forest model is not affected by AOD missingness, it can be used for epidemiological studies to estimate individual's exposure to PM2.5 at high temporal resolution without spatial or temporal gaps.

5.
Birth Defects Res ; 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33452730

RESUMO

BACKGROUND: Hypospadias is a common birth defect that might be caused by inadequate fusion of the urethral folds in the process of male external genital development. We intended to discover the crucial long noncoding RNAs (lncRNAs) regulating autophagy from the gene expression profile of the genital tubercle (GT) of dibutyl phthalate (DBP) induced hypospadiac rats. METHODS: Whole transcriptome resequencing was used to determine the expression of the total RNA in GTs and cultured fibroblasts obtained from GTs of DBP-induced hypospadiac male rat fetuses. Autophagosomes and autolysosomes were examined under a transmission electron microscope after overexpression of lncRNA NONRATT008453.2 in the fibroblasts by adenovirus transfection. Finally, the protein expression levels of Atg5, Beclin-1, Atg7, and the LC3A/B-II:LC3A/B-I ratio were detected in the fibroblasts by western blotting. RESULTS: NONRATT008453.2 suppressed autophagy by promoting the expression of Atg7, but inhibited the expressions of Atg5, Beclin-1, and the LC3A/B-II:LC3A/B-I ratio in the GT fibroblasts. CONCLUSIONS: NONRATT008453.2 may have an influence on autophagy in the fibroblasts of the GT in DBP-induced hypospadiac rats.

7.
Plant J ; 2021 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-33484592

RESUMO

The shoot apical meristems (SAMs) of land plants are crucial for plant growth and organ formation. In several angiosperms, the HAIRY MERISTEM (HAM) genes function as key regulators that control meristem development and stem cell homeostasis. To date, the origin and evolutionary history of the HAM family in land plants remains unclear. Potentially shared and divergent functions of HAM family members from angiosperms and non-angiosperms are also not known. In constructing a comprehensive phylogeny of the HAM family, we show that HAM proteins are widely present in land plants and HAM originated prior to the divergence of bryophytes. HAM duplicated in a common ancestor of angiosperms, leading to two distinct groups, the Type I and Type II. The Type II HAM members are widely present in angiosperms, whereas the Type I HAM members were independently lost in different orders of monocots. Furthermore, HAM members from angiosperms and non-angiosperms (including bryophytes, lycophytes, ferns, and gymnosperms) are able to replace the role of the Type II HAM genes in Arabidopsis, maintaining established SAMs and promoting the initiation of new stem cell niches. Our results uncover the conserved functions of HAM family members and reveal the conserved regulatory mechanisms underlying HAM expression patterning in meristems, providing insight into the evolution of key stem cell regulators in land plants.

8.
Water Res ; 188: 116465, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33049566

RESUMO

We created a hydrogen-based membrane palladium-film reactor (MPfR) by depositing palladium nanoparticles (PdNPs) on hollow-fiber membranes via autocatalytic hydrogenation to form a Pd-film. The MPfR was used for hydrodechlorination (HDC) of 2,4-dichlorophenol (2,4-DCP). HDC performances and mechanisms were systematically evaluated, and a continuous-flow dechlorination model was established. Approximately 87% of the input 2,4-DCP was reduced to the end-product phenol (P), while 2-chlorophenol (2-CP) was an intermediate, but only at 2%. Selective adsorption of the 2,4-DCP onto the Pd-film and fast desorption of P facilitated efficient dechlorination. Modeling results represented well the concentrations of 2,4-DCP and its intermediates. It demonstrated three dechlorination pathways: The majority of 2,4-DCP was completely dechlorinated to P in an adsorbed state without release of monochlorphenol, some 2,4-DCP was degraded to 2-CP that was released and subsequently adsorbed and reduced to P, and a small amount was reduced to 4-CP that was released and subsequently adsorbed and reduced to P. Analysis based on Density Functional Theory suggests that the pathway of full dechlorination was dominant due to its thermodynamically favorable adsorption configuration, with both Cl atoms bonded to Pd. This study documents full dechlorination of 2,4-DCP in the MPfR and the interacting roles of adsorption and HDC.


Assuntos
Clorofenóis , Nanopartículas Metálicas , Poluentes Químicos da Água , Hidrogênio , Membranas , Filmes Cinematográficos , Paládio , Fenóis
9.
Environ Res ; 192: 110259, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33002504

RESUMO

BACKGROUND: Cadmium is a recognized human carcinogen, raising global concern for its ubiquitously environmental exposure on public health. Diabetogenic effects of cadmium have been suggested in previous studies, but the longitudinal associations of chronic cadmium exposure with fasting blood glucose changes and type 2 diabetes mellitus have not been fully elucidated. OBJECTIVE: To investigate the effects of long-term cadmium exposure on the fasting blood glucose changes and type 2 diabetes mellitus risk in a longitudinal prospective study of China. METHODS: A total of 3521 urban adults were included as baseline study population from the Wuhan-Zhuhai cohort, and followed up three years later. Urinary cadmium concentrations were determined repeatedly during the follow-up of a three-year period. The within-person and between-person variability of urinary cadmium concentrations over three years was estimated using multilevel random-effects mixed models. Multivariate regression models were performed to evaluate the associations of cadmium exposure with fasting blood glucose changes and type 2 diabetes mellitus risk. RESULTS: The geometric means of creatinine-corrected urinary cadmium concentration at baseline were 1.13 µg/g creatinine, which were close to the levels of follow-up (1.14 µg/g creatinine). The intra-class correlation coefficient of creatinine-corrected urinary cadmium concentrations was 0.71, achieving good reproducibility of cadmium over three years. With adjustment for potential confounders, each one-unit increase in log10-transformed cadmium was associated with a 0.11 (95%CI: 0.03 to 0.19) elevation in fasting blood glucose concentration, and was associated with a 42% (95%CI: 1.16 to 1.73) increase in risk of prevalent type 2 diabetes mellitus. Upward trends of fasting blood glucose changes and type 2 diabetes mellitus incidence were observed with increasing cadmium exposure. Individuals with the highest urinary cadmium exposure had a significant increase in fasting blood glucose change at follow-up [ß (95% CI): 0.49 (0.31-0.67)]. Risk of incident type 2 diabetes mellitus were gradually elevated across increasing quartiles of cadmium exposure, though associations did not reach statistical significance (P = 0.15). CONCLUSIONS: Our findings suggested that relatively high chronic cadmium exposure for general population adults might contribute to elevated changes of fasting blood glucose resulting in the development of type 2 diabetes mellitus.

10.
J Hazard Mater ; 401: 123278, 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-32634658

RESUMO

Previous studies found that exposure to polycyclic aromatic hydrocarbons (PAHs) was associated with type 2 diabetes (T2D) prevalence. However, the potential mechanism is still unclear. In this study, we investigated 3031 Chinese urban adults to discover the relationship between PAH exposure and plasma Interleukin-22 (IL-22) and potential role of IL-22 in the association between PAH and fasting plasma glucose (FPG) or risk of T2D. After adjustment for potential confounders, significant dose-response relationships were observed between several urinary PAH metabolites with FPG and the prevalence of T2D. Each 1-U increase in ln-transformed value of 2-hydroxynaphthalene (2-OHNa), 2-hydroxyphenanthrene (2-OHPh), 3-hydroxyphenanthrene (3-OHPh), 4-hydroxyphenanthrene (4-OHPh), 9-hydroxyphenanthrene (9-OHPh), 1-hydroxypyrene (1-OHP) or total PAH metabolites was significantly associated with a 0.053, 0.026, 0.037, 0.045, 0.051, 0.041 or 0.047 unit decrease in IL-22 level, respectively. In addition, plasma IL-22 level was negatively associated with FPG and prevalence of T2D in a dose-dependent manner. Mediation analysis showed that IL-22 mediated 8.48 %, 3.87 %, 6.64 %, 6.47 %, and 8.67 % of the associations between urinary 2-OHNa, 1-OHPh, 3-OHPh, 4-OHPh, and 9-OHPh with the prevalence of T2D, respectively. These results indicated that urinary PAHs metabolites were inversely associated with plasma levels of IL-22, but positively related to FPG and the T2D prevalence. Downregulation of IL-22 might play a significant role in mediating PAHs exposure-associated risk increasement of T2D.

11.
Entropy (Basel) ; 22(8)2020 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-33286663

RESUMO

Stealth malware is a representative tool of advanced persistent threat (APT) attacks, which poses an increased threat to cyber-physical systems (CPS) today. Due to the use of stealthy and evasive techniques, stealth malwares usually render conventional heavy-weight countermeasures inapplicable. Light-weight countermeasures, on the other hand, can help retard the spread of stealth malwares, but the ensuing side effects might violate the primary safety requirement of CPS. Hence, defenders need to find a balance between the gain and loss of deploying light-weight countermeasures, which normally is a challenging task. To address this challenge, we model the persistent anti-malware process as a shortest-path tree interdiction (SPTI) Stackelberg game with both static version (SSPTI) and multi-stage dynamic version (DSPTI), and safety requirements of CPS are introduced as constraints in the defender's decision model. The attacker aims to stealthily penetrate the CPS at the lowest cost (e.g., time, effort) by selecting optimal network links to spread, while the defender aims to retard the malware epidemic as much as possible. Both games are modeled as bi-level integer programs and proved to be NP-hard. We then develop a Benders decomposition algorithm to achieve the Stackelberg equilibrium of SSPTI, and design a Model Predictive Control strategy to solve DSPTI approximately by sequentially solving an 1+δ approximation of SSPTI. Extensive experiments have been conducted by comparing proposed algorithms and strategies with existing ones on both static and dynamic performance metrics. The evaluation results demonstrate the efficiency of proposed algorithms and strategies on both simulated and real-case-based CPS networks. Furthermore, the proposed dynamic defense framework shows its advantage of achieving a balance between fail-secure ability and fail-safe ability while retarding the stealth malware propagation in CPS.

12.
EMBO J ; : e104615, 2020 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-33280146

RESUMO

The BRASSINAZOLE-RESISTANT 1 (BZR1) transcription factor family plays an essential role in plant brassinosteroid (BR) signaling, but the signaling mechanism through which BZR1 and its homologs cooperate with certain coactivators to facilitate transcription of target genes remains incompletely understood. In this study, we used an efficient protein interaction screening system to identify blue-light inhibitor of cryptochromes 1 (BIC1) as a new BZR1-interacting protein in Arabidopsis thaliana. We show that BIC1 positively regulates BR signaling and acts as a transcriptional coactivator for BZR1-dependent activation of BR-responsive genes. Simultaneously, BIC1 interacts with the transcription factor PIF4 to synergistically and interdependently activate expression of downstream genes including PIF4 itself, and to promote plant growth. Chromatin immunoprecipitation assays demonstrate that BIC1 and BZR1/PIF4 interdependently associate with the promoters of common target genes. In addition, we show that the interaction between BIC1 and BZR1 is evolutionally conserved in the model monocot plant Triticum aestivum (bread wheat). Together, our results reveal mechanistic details of BR signaling mediated by a transcriptional activation module BIC1/BZR1/PIF4 and thus provide new insights into the molecular mechanisms underlying the integration of BR and light signaling in plants.

13.
Environ Pollut ; 267: 115647, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33254652

RESUMO

Copper have been reported to be associated with metabolic diseases. However, results on copper exposure with blood lipid profiles are inconsistent, and the underlying mechanisms of this association remain unclear. This study focused on investigating associations between urinary copper and blood lipid profiles; and exploring the potential role of systemic inflammation in such relationships. Concentrations of urinary copper, plasma C-reactive protein (CRP), and four blood lipid parameters (e.g., Total cholesterol [TC], triglycerides [TG], low-density lipoprotein cholesterol [LDL-C], and high-density lipoprotein cholesterol [HDL-C]) were measured in the adult participants from Wuhan-Zhuhai cohort. The associations between copper, CRP, and four blood lipids were assessed by the multivariable linear regression models, and the 3D mesh graphs was used to examine the joint effects of copper exposure and CRP on four blood lipid parameters. In addition, we used mediation analysis to investigate the mediated effects of CRP in the relationships between copper exposure and blood lipid profiles. Each 1% increase in urinary copper was statistically significantly associated with a 5.32% (95% CI: 2.48%, 8.24%) increase in TG after adjusting for the confounders (P < 0.05). No significant associations were observed between urinary copper and the other three blood lipid parameters (all P > 0.05). In addition, urinary copper increased monotonically with plasma CRP elevation, which in turn, was positively associated with TC, TG, and LDL-C and negatively related to HDL-C (all P < 0.05). Results from 3D mesh graphs demonstrated that increased levels of plasma CRP with higher urinary copper corresponded to higher TC, TG, LDL-C, and lower HDL-C concentrations. Mediation analysis observed that CRP mediated 6.27% in the relationships of urinary copper and TG. These findings suggest that systemic inflammation partly mediated the association between copper exposure and abnormal blood lipid, and may contribute to the development of dyslipidemias.

14.
Front Cell Infect Microbiol ; 10: 588107, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33324575

RESUMO

Rodents constitute the largest and most successful group of mammals worldwide. Brown rats (Rattus norvegicus) are one of the most common rodent species, and they serve as intermediate hosts of Hydatigera taeniaeformis. Although there have been a few studies reporting on the presence of the larval form of H. taeniaeformis (strobilocercus fasciolaris) in brown rats worldwide, little information is available on the genetic characterization of this parasite, with no molecular data from China. Therefore, from April 2014 to March 2016, this study was carried out to understand the prevalence and genetic characters of strobilocercus fasciolaris in brown rats captured in Heilongjiang Province in northeastern China. The livers of brown rats were collected and examined for the presence of cysts. Each cyst was identified based on morphological observation: the larvae with the naked eye and the scolexes under a microscope. The results were confirmed by polymerase chain reaction (PCR) and sequencing of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 4 (nad4) genes. At the investigated sites, 11.8% (13/110) of the brown rats were infected with strobilocercus fasciolaris. Based on sequence analysis, there were 10 and six haplotypes regarding the cox1 and the nad4 loci, with 24 and 42 polymorphic sites, respectively (degree of intraspecific variation: 0.3%-4.4% and 0.6%-4.7%, respectively). Twelve nucleotide sequences (six of the 10 at the cox1 locus and all six at the nad4 locus) have not previously been described. Base differences in three of the six novel cox1 gene sequences and five of the six novel nad4 gene sequences caused amino acid changes. Phylogenetic analyses of the cox1 and nad4 gene sequences based on neighbor-joining and Bayesian inference trees indicated that all the strobilocercus fasciolaris isolates belonged to Hydatigera taeniaeformis sensu stricto (s.s.). This is the first report on the genetic characterization of strobilocercus fasciolaris in brown rats in China. The findings of novel cox1 and nad4 nucleotide and amino acid sequences may reflect the region-specific genetic characterization of the parasite. The data will be useful to explore the biological and epidemiological significance of the intraspecific variation within H. taeniaeformis s.s.

15.
BMC Endocr Disord ; 20(1): 182, 2020 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-33317485

RESUMO

OBJECTIVE: Diabetic ketoacidosis (DKA) and hyperosmolar hyperglycemic state (HHS) are the two most common hyperglycemic emergencies (HEs) associated with diabetes mellitus. Individuals with HEs can present with combined features of DKA and HHS. The objective of this study is to assess the clinical characteristics, therapeutic outcomes, and associated predisposing factors of type 2 diabetic patients with isolated or combined HEs in China. METHODS: We performed a retrospective analysis of 158 patients with type 2 diabetes (T2DM), complicated with DKA, HHS, or DKA combined with HHS (DKA-HHS) in Shanghai Tongji Hospital, China from 2010 to 2015. Admission clinical features, therapeutic approaches and treatment outcomes of those patients were extracted and analyzed. RESULTS: Of the 158 patients with T2DM, 65 (41.1%) patients were DKA, 74 (46.8%) were HHS, and 19 (12.0%) were DKA-HHS. The most common precipitants were infections (111, 70.3%), newly diagnosed diabetes (28,17.7%) and non-compliance to medications (9, 5.7%). DKA patients were divided into mild, moderate and severe group, based on arterial blood gas. Spearman correlation analysis revealed that C-reaction protein (CRP) was positively correlated with severity of DKA, whereas age and fasting C peptide were inversely correlated with severity of DKA (P < 0.05). The mortality was 10.8% (17/158) in total and 21.6% (16/74) in the HHS group, 5.9% (1/17) in DKA-HHS. Spearman correlation analysis indicated that death in patients with HHS was positively correlated to effective plasma osmolality (EPO), renal function indicators and hepatic enzymes, while inversely associated with the continuous subcutaneous insulin infusion (CSII) therapy. Logistic regression analysis suggested that elevated blood urea nitrogen (BUN) on admission was an independent predisposing factor of mortality in HHS, while CSII might be a protective factor for patients with HHS. Furthermore, the receiver-operating characteristic (ROC) curve analysis indicated that BUN had the largest area under the ROC curves for predicting death in patients with HHS. CONCLUSIONS: Our findings showed elevated CRP and decreased fasting C-peptide might serve as indicator for severe DKA. Elevated BUN might be an independent predictor of mortality in patients with HHS, whereas CSII might be a protective factor against death in HHS.

16.
Neurochem Int ; 143: 104929, 2020 Dec 23.
Artigo em Inglês | MEDLINE | ID: mdl-33359189

RESUMO

During spinal cord injury (SCI), a quick and sustained decline of Neuregulin-1 (Nrg1) has been observed, exerting a significant positive effect in modulating the proliferation of astrocytes and the formation of glial scars within the damaged spinal cord. In this study, we revealed the abnormal downregulation of lncRNA Ftx and Nrg1 and upregulation of miR-382-5p after SCI, which contributed to the inflammatory response in microglial cells and affected SCI repair. Ftx overexpression was significantly reduced, and Ftx knockdown further promoted LPS effects on the inflammatory factors, indicating that lncRNA Ftx might affect the microglial inflammatory response. miR-382-5p targeted both lncRNA Ftx and Nrg1, and lncRNA Ftx competed with Nrg1 for miR-382-5p binding to act as a ceRNA, therefore counteracting miR-382-5p-mediated inhibition of Nrg1. miR-382-5p overexpression was significantly enhanced, and Nrg1 overexpression attenuated LPS effects on inflammatory factors within the microglia. Under LPS stimulation, the effects of Ftx overexpression were significantly reversed by overexpression of miR-382-5p, and the effects of miR-382-5p overexpression were significantly reversed by Nrg1 overexpression. In summary, the lncRNA Ftx/miR-382-5p/Nrg1 axis improves the inflammation response of the microglia, which might improve SCI repair.

17.
Cancer Manag Res ; 12: 10705-10713, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33149677

RESUMO

Introduction: Pancreatic cancer (PC) is a malignant tumor with poor prognosis. This study aimed to determine the role of trefoil factor 2 (TFF2) in the proliferation and apoptosis of LPS-induced normal pancreatic duct cells and pancreatic cancer cells through ß-catenin pathway. Methods: TFF2 expression in normal pancreatic duct cells, pancreatic cancer cells and LPS-induced normal pancreatic duct cells was detected by RT-qPCR analysis and Western blot analysis. The transfection effects in pancreatic cancer cells and LPS-induced normal pancreatic duct cells were analyzed by RT-qPCR analysis. After indicated transfection, proliferation, apoptosis and inflammation of these cells were respectively detected by CCK-8 assay, TUNEL assay and certain ELISA kits. Expression of ß-catenin pathway-related proteins was analyzed by Western blot analysis. Co-immunoprecipitation assay determined the combination of TFF2 and ß-catenin. Results: TFF2 expression was increased in pancreatic cancer cells and LPS-induced HPDE cells compared with HPDE cells. According to TFF2 expression in these cells, PanC-1 cells and 5 µg/mL LPS were selected. In addition, TFF2 interference decreased the proliferation and promoted the apoptosis of PanC-1 cells and LPS-induced HPDE cells. However, TFF2 interference did not obviously change the levels of TNF-α, IL-1ß and IL-6 in PanC-1 cells and LPS-induced HPDE cells. Furthermore, TFF2 interference suppressed the expression of ß-catenin, c-Myc, Cyclin D1 and BIRC5 in PanC-1 cells and LPS-induced HPDE cells. TFF2 was demonstrated to combine with ß-catenin. Discussion: TFF2 interference inhibits proliferation and promotes apoptosis of PanC-1 cells and LPS-induced HPDE cells by suppressing ß-catenin pathway.

18.
Materials (Basel) ; 13(21)2020 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-33137981

RESUMO

The surface crack of ballastless track slab can seriously reduce the serviceability and durability of high-speed railway (HSR). Aiming at accurately and efficiently detecting the slab cracks, this research proposes an infrared thermography (IRT)-based method for the surface crack, which is the most serious and common crack type in track slab. A three dimensional finite element (FE) model of IRT detection of concrete slab with surface cracks is established. The relation between the width of detectable cracks and the ambient temperature can be thereby obtained by inputting the measured thermodynamic parameters in the model. Parametric study shows that with ambient temperature higher than 15 °C, cracks with a width of no less than 0.2 mm can be well detected. Scale model test and field test are conducted, IRT method can effectively locate the slab surface cracks with width as small as 0.14 mm when ambient temperature is no less than 20 °C.

19.
Artigo em Inglês | MEDLINE | ID: mdl-33245515

RESUMO

Diabetic retinopathy (DR) is a serious microvascular complication of diabetes. Gambogic acid has been reported to have anti-inflammatory effect. However, the effect of GA on inflammatory response of ARPE-19 cells remains unclear. In our study, ARPE-19 cells were stimulated by palmitic acid (PA) induction in the presence of 30 mM glucose and then treated with 0.5, 1, 2, 5, 10, or 20 µM GA. CCK-8 assay showed that cell viability was increased by GA treatment at doses of 0.5, 1, and 2 µM instead of higher doses. ELISA analysis found that GA dose-dependently reduced the production of pro-inflammatory mediators TNF-α and IL-1ß. Western blot indicated that GA downregulated the expression of NLRP3 inflammasome components including TXNIP, NLRP3, ASC, cleaved-caspase-1, and cleaved-IL-1ß in a dose-dependent manner. In addition, Western blot and immunofluorescence analysis suggested that GA effectively increased the protein level of nuclear factor E2-related factor-2 (Nrf2). RT-qPCR showed that GA significantly increased the mRNA levels of Heme oxygenase-1 (HO-1) and NADPH:quinone oxidoreductase1 (NQO1). Furthermore, Nrf2 siRNA transfection confirmed the above effects of GA. In total, subtoxic doses of GA significantly flattened the inflammatory response induced by HG and PA in ARPE-19 cells via modulating the Nrf2 signaling pathway.

20.
Chem Commun (Camb) ; 56(93): 14617-14620, 2020 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-33151211

RESUMO

A straightforward and efficient method for the preparation of 2-aryl-2H-indazoles from ortho-alkyl substituted azoxybenzenes is presented. The reaction proceeds through base-catalyzed benzyl C-H deprotonation and cyclization to afford 2-aryl-2H-indazoles in good yields. This synthetic strategy can be applied to the construction of several fluorescent and bioactive molecules.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA