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1.
Int J Nanomedicine ; 17: 4119-4135, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36118178

RESUMO

Pharyngocutaneous fistula is the most common complication after total laryngectomy and is difficult to heal. Although conservative treatment and surgical repair are effective, they often take longer and additional trips to the operating room, which undoubtedly increases the financial burden on patients. Especially in combination with diseases such as diabetes and hypertension, which affect the efficacy of surgery. Adding growth factors into the repair material can promote fibroblast proliferation, angiogenesis, and accelerate wound healing. A substantial number of studies have shown that a type of nanoscale extracellular vesicle, called exosomes, facilitates organization repair by promoting blood vessel production, protein polysaccharides, and collagen deposition, thereby representing a new type of cellular therapy. At present, there is little research on the application of exosomes in pharyngocutaneous fistula regeneration after total laryngectomy. In this review, we summarize the biological characteristics of exosomes and their application in biomedical science, and highlight their application prospects in pharyngocutaneous fistula regeneration after total laryngectomy.


Assuntos
Fístula Cutânea , Exossomos , Neoplasias Laríngeas , Doenças Faríngeas , Fístula Cutânea/complicações , Fístula Cutânea/terapia , Humanos , Laringectomia/efeitos adversos , Doenças Faríngeas/etiologia , Doenças Faríngeas/cirurgia
2.
Liver Int ; 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36069651

RESUMO

BACKGROUND AND AIMS: Epidemiological evidence regarding the association of air pollution with the risk of non-alcoholic fatty liver disease (NAFLD) is limited. This study was to examine the associations of long-term exposure to various air pollutants and overall air pollution with risk of incident NAFLD as well as cirrhosis, a major liver-related morbidity for NAFLD. METHODS: Included were 456 687 UK residents. Air pollution data included PM2.5 , PM2.5-10 , PM10 , NO2 and NOx . A weighted air pollution score was also generated from PM10 and NOx . Cox proportional hazard models were employed to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs). RESULTS: We identified 4978 cases of NAFLD and 1575 cases of incident cirrhosis, over a median follow-up of 11.9 years. PM2.5 , PM10 , NO2 and NOx exposures contributed to the excess risk of NAFLD associated with air pollution score; and the corresponding adjusted HRs (95% CI) were 1.10 (1.05, 1.14), 1.14 (1.09, 1.20), 1.19 (1.13, 1.24) and 1.11 (1.07, 1.15), respectively, for each interquartile range increase in the above specific air pollutants. Similar patterns were also indicated for cirrhosis risk. Alcohol consumption was an effect modifier for the association between air pollution score and NAFLD risk, whereas body mass index modified the association for cirrhosis risk. CONCLUSION: Long-term exposure to air pollution was associated with risks of NAFLD and cirrhosis among the UK population.

3.
Zootaxa ; 5133(2): 270-278, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-36101099

RESUMO

Although some females of Plecoptera are described in taxonomic literature, they are difficult to morphologically distinguish among regional congeners, and identifications are often made through inference only. Therefore, females of many species are unknown and it is often difficult to match males and females correctly. Recently, we reviewed the specimens of the genus Rhopalopsole Klaplek, 1912 that we collected and found that differentiation between female subgenital plates is significant and very helpful for the identification of species. A new species of this genus from Sichuan Province, Rhopalopsole singiplatta Yang Du, sp. nov., is established based on morphological and molecular data. In addition, we used modern tree- and genetic distance-based phylogenetic methods to positively associate females with males for six species in total.


Assuntos
Insetos , Neópteros , Animais , Feminino , Masculino , Filogenia
4.
Med Biol Eng Comput ; 60(10): 3029-3040, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36053430

RESUMO

Accurate diagnosis and surgical selection of the double-outlet right ventricle (DORV) is both critical and difficult. Virtual models and three-dimensional (3D) printing have been used to provide morphological copies to doctors as reference. However, the existing methods have shortcomings in visualization of the surgical results, optimal surgical design, and accurate surgical scheme measurements. To overcome this problem, we performed surgical predictions by designing the intraventricular baffle and ventricular septal defect patch to evaluate surgical options and using 3D printing to guide the trimming of the baffle or patch. A complete set of processes including scanning, modeling, designing, 3D printing, and guiding the trimming of the baffle for the diagnosis and surgical planning of DORV was established. Six cases were used to evaluate the feasibility of this method. The average rate of misdiagnosis of the six cases by computed tomography and echocardiography was 42.5%, which was reduced to 4.6% when the diagnosis was established using the virtual models and 3D printing as auxiliary tools. The approach effectively improved diagnostic accuracy, guided the operation, and simplified the process of patch trimming. The proposed method can thus be used for improving the surgical simulation and guiding of the DORV surgery.


Assuntos
Dupla Via de Saída do Ventrículo Direito , Comunicação Interventricular , Dupla Via de Saída do Ventrículo Direito/cirurgia , Ecocardiografia , Estudos de Viabilidade , Comunicação Interventricular/cirurgia , Humanos , Impressão Tridimensional
5.
Animals (Basel) ; 12(17)2022 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-36077903

RESUMO

Underwater noise pollution caused by human activities may affect freshwater cetaceans to different degrees. To analyze the impacts of water intake pontoons on Yangtze finless porpoises (Neophocaena asiaeorientalis), this study collected underwater noise data from such a pontoon in a nature reserve, plotted the power spectral density of acoustic signals, and calculated the root mean square sound pressure levels and the magnitude of sound source levels. The 1/3-octave sound pressure level root mean square values at the transient holding pens were <18.0 kHz, 39.5-60.0 kHz, which were slightly higher than the Yangtze finless porpoise hearing threshold curve values and therefore could be perceived. However, the results indicated that the porpoises would not develop a temporary hearing threshold shift. Meanwhile, pontoon noise did not interfere with the porpoises' high-frequency acoustic signal nor did it affect their echolocation; it significantly interfered with their low-frequency acoustic signal, however, and the mother-child communication of the finless porpoises was affected, but this effect was quickly compensated due to the limited space range of the holding pens. Through this study of Yangtze finless porpoises, this paper provides a reference for assessing whether human facilities have impacts on freshwater cetaceans.

6.
BMC Musculoskelet Disord ; 23(1): 835, 2022 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-36057592

RESUMO

BACKGROUND: A symptomatic postoperative pseudocyst (PP) is a cystic lesion that is formed in the operation area of the intervertebral disc, leading to worse symptoms. Some minority patients who developed PP experienced rapidly aggravating symptoms and could not be treated by any kind of conservative treatment. However, no clinical studies have evaluated the clinical characteristics and surgical strategies of symptomatic PP requiring a revision surgery after full-endoscopic lumbar discectomy (FELD). This study aimed to demonstrate the clinical characteristics and surgical strategies of symptomatic PP requiring a revision surgery after FELD. METHODS: We retrospectively analyzed the data of patients who received FELD revision surgeries due to symptomatic PP formation between January 2016 and December 2021. Common characteristics, time intervals of symptom recurrence and revision surgery, strategies for conservative treatment and revision surgery, operative time, imaging characteristics, numeric rating scale (NRS) score, Oswestry disability index (ODI) and overall outcome rating based on modified MacNab criteria were analyzed. RESULTS: Fourteen patients (males = 10, females = 4), with a mean age of 24.4 years, were enrolled. The mean time intervals of symptom recurrence and revision surgery were 43.5 and 18.9 days respectively. While the patients were conservatively managed with analgesics and physical therapy, pain persisted or progressively worsened. In comparison to the initial herniated disc, the PP was larger in 11 cases, and up- or down-migrated in four cases. The PP location included the lateral recess (n = 12), foraminal (n = 1), and centrolateral (n = 1) zones. One of the two cases treated by percutaneous aspiration (PA) was eventually treated by FELD as pain was not relieved. Follow-ups revealed an improved mean NRS score from 7.1 to 1.4, mean ODI from 68.6 to 7.9% and promising overall surgical outcomes. CONCLUSIONS: The progressively severe pain experienced due to PP might be a result of its enlargement or migration to the lateral recess and foraminal zones. As complete removal of capsule is the goal, we recommend FELD instead of PA.


Assuntos
Discotomia Percutânea , Deslocamento do Disco Intervertebral , Adulto , Discotomia/efeitos adversos , Discotomia/métodos , Discotomia Percutânea/efeitos adversos , Discotomia Percutânea/métodos , Endoscopia/efeitos adversos , Endoscopia/métodos , Feminino , Humanos , Deslocamento do Disco Intervertebral/etiologia , Deslocamento do Disco Intervertebral/cirurgia , Vértebras Lombares/diagnóstico por imagem , Vértebras Lombares/cirurgia , Masculino , Dor/etiologia , Reoperação , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
7.
BMC Infect Dis ; 22(1): 723, 2022 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-36064333

RESUMO

BACKGROUND: The prevalence of infectious diseases remains one of the major challenges faced by the Chinese health sector. Policymakers have a tremendous interest in investigating the spatiotemporal epidemiology of infectious diseases. We aimed to review the small-scale (city level, county level, or below) spatiotemporal epidemiology of notifiable infectious diseases in China through a systematic review, thus summarizing the evidence to facilitate more effective prevention and control of the diseases. METHODS: We searched four English language databases (PubMed, EMBASE, Cochrane Library, and Web of Science) and three Chinese databases (CNKI, WanFang, and SinoMed), for studies published between January 1, 2004 (the year in which China's Internet-based disease reporting system was established) and December 31, 2021. Eligible works were small-scale spatial or spatiotemporal studies focusing on at least one notifiable infectious disease, with the entire territory of mainland China as the study area. Two independent reviewers completed the review process based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. RESULTS: A total of 18,195 articles were identified, with 71 eligible for inclusion, focusing on 22 diseases. Thirty-one studies (43.66%) were analyzed using city-level data, 34 (47.89%) were analyzed using county-level data, and six (8.45%) used community or individual data. Approximately four-fifths (80.28%) of the studies visualized incidence using rate maps. Of these, 76.06% employed various spatial clustering methods to explore the spatial variations in the burden, with Moran's I statistic being the most common. Of the studies, 40.85% explored risk factors, in which the geographically weighted regression model was the most commonly used method. Climate, socioeconomic factors, and population density were the three most considered factors. CONCLUSIONS: Small-scale spatiotemporal epidemiology has been applied in studies on notifiable infectious diseases in China, involving spatiotemporal distribution and risk factors. Health authorities should improve prevention strategies and clarify the direction of future work in the field of infectious disease research in China.


Assuntos
Doenças Transmissíveis , China/epidemiologia , Doenças Transmissíveis/epidemiologia , Humanos , Incidência , Prevalência , Fatores de Risco
8.
Healthcare (Basel) ; 10(9)2022 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-36141223

RESUMO

Lip and oral cavity cancer is a common malignancy faced by many developing countries, and the disease burden is high in China. This study explored this cancer burden and its risk factors using data from China in the GBD 2019, along with predicting the incidence trends in 2020-2049. Data on age-standardized rates (ASR), incidence, death and disability-adjusted life years (DALY), by sex, age and risk factors were collected from the Institute for Health Metrics and Evaluation (IHME). Joinpoint regression and Age-Period-Cohort (APC) models were selected to analyze the epidemic trend of this cancer in China, and descriptive analysis was used for the time trend and age distribution of risk factors. The Bayesian APC model was selected to foresee the incidence trend in 2020-2049. This cancer burden was found to be in an upward trend in China in 1990-2019. The upward trend was more pronounced among men than among women. These cancer deaths and DALYs are overwhelmingly attributable to smoking and drinking. On APC analysis, the younger generation in China demonstrated a lower cancer risk. In 2049, the incidence of this cancer is projected to be 3.99/100,000, 6.07/100,000, 7.37/100,000, 10.49/100,000, 14.82/100,000, 19.19/100,000, 20.71/100,000, 23.64/100,000, 16.42/100,000 and 9.91/100,000 among those aged 50-54, 55-59, 60-64, 65-69, 70-74, 75-79, 80-84, 85-89, 85-89 and over 95 years, respectively. Disease control policies and early screening should focus on men and the elderly and target different risk factors.

9.
Healthcare (Basel) ; 10(9)2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36141337

RESUMO

On 14 March 2022, China's tech hub Shenzhen, a mega-city with more than 18 million inhabitants, imposed a one-week citywide lockdown immediately after it observed a surge in infections. We assessed the effect of this one-week lockdown, coupled with mass testing, on reducing the daily number of new confirmed cases and asymptomatic cases during the Omicron wave, using an interrupted time series analysis approach. Our analysis suggests that the one-week citywide lockdown in Shenzhen was effective at lowering both daily new confirmed cases and asymptomatic cases during the Omicron wave. Early detection ensures timely isolation and treatment of infected patients in designated hospitals, and therefore helps lower the prevalence of confirmed cases and asymptomatic cases. Our findings of the immediate increase in asymptomatic cases after lockdown warrant further verifications in other city epidemic scenarios.

10.
Sensors (Basel) ; 22(18)2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36146126

RESUMO

Orthotropic steel decks (OSDs) are inevitably subjected to fatigue damage caused by cycled vehicle loads in long-span bridges. This study establishes a probabilistic analysis framework integrating the dynamic Bayesian network (DBN) and fracture mechanics to model the fatigue crack propagation considering mutual correlations among multiple fatigue details. Both the observations of fatigue crack length from field inspection and monitoring data of vehicle loads from the weight-in-motion (WIM) system are utilized. First, fracture mechanics-based uncertainty analysis is performed to determine the multiple uncertainty sources in the Paris crack propagation model, material property, and observation data of crack length. The uncertainty of monitoring data of vehicle loads is ignored because of its high accuracy; consequently, the vehicle-load-related uncertainty is spontaneously ignored, which is also demonstrated to be very small on the investigated actual bridges. Second, a hierarchical DBN model is introduced to construct mutual dependencies among various uncertainties and intra-correlations in the propagation process of multiple fatigue cracks at different components. Third, a stochastic traffic model is established based on the WIM monitoring data and multi-scale finite element analysis via substructure techniques to determine the probability distribution of vehicle-load-related parameters. After variable discretization, a refined exact inference algorithm in a forward-backward-forward manner is adopted to estimate the posterior distribution of equivalent initial crack length and update the established DBN model. Finally, the proposed method is demonstrated by a numerical case study and a typical application on an actual cable-stayed bridge with steel box girders using OSDs in China. The results show that the probability distribution of equivalent initial crack size can be spontaneously derived with a larger mean value than the results of conventional empirical analysis. Furthermore, the component-level fatigue reliability is tracked and predicted based on the established crack propagation model.

11.
RSC Adv ; 12(38): 24480-24490, 2022 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-36128392

RESUMO

Fast ionic conduction at low operating temperatures is a key factor for the high electrochemical performance of solid oxide fuel cells (SOFCs). Here an A-site deficient semiconductor electrolyte Sr1-x Co x FeO3-δ is proposed for low-temperature solid oxide fuel cells (LT-SOFCs). A fuel cell with a structure of Ni/NCAL-Sr0.7Co0.3FeO3-δ -NCAL/Ni reached a promising performance of 771 mW cm-2 at 550 °C. Moreover, appropriate doping of cobalt at the A-site resulted in enhanced charge carrier transportation yielding an ionic conductivity of >0.1 S cm-1 at 550 °C. A high OCV of 1.05 V confirmed that neither short-circuiting nor power loss occurred during the operation of the prepared SOFC device. A modified composition of Sr0.5Co0.5FeO3-δ and Sr0.3Co0.7FeO3-δ also reached good fuel cell performance of 542 and 345 mW cm-2, respectively. The energy bandgap analysis confirmed optimal cobalt doping into the A-site of the prepared perovskite structure improved the charge transportation effect. Moreover, XPS spectra showed how the Co-doping into the A-site enhanced O-vacancies, which improve the transport of oxide ions. The present work shows that Sr0.7Co0.3FeO3-δ is a promising electrolyte for LT-SOFCs. Its performance can be boosted with Co-doping to tune the energy band structure.

12.
Phys Chem Chem Phys ; 2022 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-36131634

RESUMO

Cerium oxide based ceramic fuel cells (CFCs) enable a good cell performance with high ionic conductivity when a lithium compound is utilized as the anode material. However, the mechanism of enhancement of the ionic conductivity and its effect on the fuel cell performance as well as the stability involved via the lithium effect have not been fully understood in this stage. In this paper, the role of lithium was unveiled through experimental measurements and DFT calculations in cerium oxide-based CFCs. It is found that the redistribution of lithium in cerium oxide causes gradient Li+ distribution, resulting in the diffusion of Li+ in CeO2 electrolyte to improve the cell performance. Further study discloses that the lithium at the anode is depleted and in situ doped into the cerium oxide lattice, modulating the band structure of CeO2, leading to the increased electronic conductivity and open circuit voltage (OCV) degradation. This work provides an insight into the role of lithium in cerium oxide-based CFCs, opening a new methodology for designing high performance CFCs.

13.
J Wound Care ; 31(9): 800-804, 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36113546

RESUMO

OBJECTIVE: Platelet-rich fibrin (PRF) plays a role in promoting wound healing by releasing cytokines, chemokines and growth factors, and by inducing proliferation and activation of cells. A pressure injury (PI) is a treatable but serious and costly disease with adverse outcomes for the patient. However, traditional PI treatments are time-consuming, with limited effectiveness. Thus, we aimed to investigate the effects and mechanisms of PRF on skin wound healing in PIs in vivo in a rat model. METHOD: PRF was prepared from the blood of male Wistar rats. A rat model for PI ischaemia/reperfusion injury was established by placing a magnet onto the back skin, where a magnetic steel plate had been previously implanted. The rats were randomised into two groups: the control group was treated with sterile gauze dressings and the iPRF group received additional PRF. Skin wound healing rate was calculated and a CD31/Masson's trichrome stain performed. RESULTS: In this study, 16 rats were allocated to the two groups (n=8 in each group). PRF improved the skin wound healing rate of PIs in the rats; haematoxylin and eosin staining and CD31 staining showed that the number of capillaries increased significantly in the wound. However, Masson's trichrome staining showed no increase in fibrotic tissues after PRF treatment. CONCLUSION: In this in vivo rat model for PI, PRF accelerated skin wound healing by increasing angiogenesis in the wound.


Assuntos
Fibrina Rica em Plaquetas , Animais , Citocinas , Amarelo de Eosina-(YS)/farmacologia , Masculino , Ratos , Ratos Wistar , Aço/farmacologia , Cicatrização/fisiologia
14.
Microbiol Spectr ; : e0308522, 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36073822

RESUMO

Spring viremia of carp virus (SVCV) is highly contagious and lethal to most cyprinid fish, causing serious economic losses to the carp aquaculture industry. Although DNA vaccines can generate long-term humoral and cellular immune responses, which provide protective immunity against SVCV, the major drawback of DNA vaccines is their low immunogenicity in clinical tests. Here, we construct a dual-targeted polymer DNA vaccine delivery platform (MCS-PCHG) by using mannosylated chitosan to encapsulate the poly(d,l-lactide-co-glycolide)-loaded DNA vaccine containing the heavy-chain CH3 region (CH3) of common carp IgM and the antigenic domain (G131c). The developed nanovaccine delivery platform showed good biocompatibility in vivo and in vitro. With the modification of the mannose moiety and the modification of CH3, the constructed MCS-PCHG could efficiently activate the maturation of antigen-presenting cells. Moreover, we observe significantly high level of immune-related genes expression, serum antigen-specific IgM, SVCV-neutralizing antibody titers in fish vaccinated with MCS-PCHG. Next, the protective efficacy of MCS-PCHG was further evaluated by challenge test. The highest survival rate (ca. 84%) was observed in fish vaccinated with MCS-PCHG after challenging with SVCV. This study presents a novel design for smart, dual-targeted polymer nanoparticles, which are inherently biocompatible, promising for targeted vaccine delivery. IMPORTANCE Spring viremia of carp virus (SVCV) affects global cyprinid fish farming industry, with no available commercial vaccine. Herein, we developed a dual-targeting polymer nanovaccine (MCS-PCHG) by using mannose and common carp IgM heavy chain CH3 region (CH3) as antigen presenting cell (APCs) recognition moiety, attaining the effective delivery of antigen. This dual-targeting polymer vaccine can efficiently activate the APCs, and further induce robust and durable adaptive immune response with good protection against SVCV infection. Our study provides valuable theoretical basis for developing efficient vaccine against infectious diseases in aquaculture.

15.
Nat Commun ; 13(1): 5284, 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36075929

RESUMO

Myelofibrosis is a rare myeloproliferative neoplasm (MPN) with high risk for progression to acute myeloid leukemia. Our integrated genomic analysis of up to 933 myelofibrosis cases identifies 6 germline susceptibility loci, 4 of which overlap with previously identified MPN loci. Virtual karyotyping identifies high frequencies of mosaic chromosomal alterations (mCAs), with enrichment at myelofibrosis GWAS susceptibility loci and recurrently somatically mutated MPN genes (e.g., JAK2). We replicate prior MPN associations showing germline variation at the 9p24.1 risk haplotype confers elevated risk of acquiring JAK2V617F mutations, demonstrating with long-read sequencing that this relationship occurs in cis. We also describe recurrent 9p24.1 large mCAs that selectively retained JAK2V617F mutations. Germline variation associated with longer telomeres is associated with increased myelofibrosis risk. Myelofibrosis cases with high-frequency JAK2 mCAs have marked reductions in measured telomere length - suggesting a relationship between telomere biology and myelofibrosis clonal expansion. Our results advance understanding of the germline-somatic interaction at JAK2 and implicate mCAs involving JAK2 as strong promoters of clonal expansion of those mutated clones.


Assuntos
Transtornos Mieloproliferativos , Mielofibrose Primária , Células Germinativas , Haplótipos , Humanos , Janus Quinase 2/genética , Mutação , Transtornos Mieloproliferativos/genética , Mielofibrose Primária/genética
16.
Environ Res ; 214(Pt 4): 114095, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36037924

RESUMO

Since the Air Pollution Prevention and Control Action Plan (air clean plan) issued in 2013, air quality has been in continuous improvement. The second stage of air clean plan since 2018 was focused on O3 controlling, but it still didn't decline so significantly as PM2.5. This study conducted a long-term observation on black carbon (BC) and utilized the observational data of other air pollutants (PM2.5, PM10, NO2, SO2, CO and O3), the meteorological elements and the vertical sounding data of PBL in Nanjing. In the daytime (08:00-20:00), PM2.5 kept decreasing from 2015 to 2020 at the rate of 4.8 µg⋅m-3⋅a-1, however, BC increased at the rate of 0.6 µg⋅m-3⋅a-1, which has led to the continuous growth of BC/PM2.5 (0.9%⋅a-1). However, during this period, O3 was relatively stable and, in 2020, it returned below its value in 2015 after slight increases in 2017 and 2018. Meanwhile, the average surface temperature had increased by around 1.0 °C during 2015-2019 at the rate of 0.3 °C⋅a-1. Also, the average height of the inversion layer had increased significantly by 494.0 and 176.7 m at 20:00 and 08:00, whose growth ratio was up to 57% and 25%, respectively. The above observation results have formed a set of chain reactions as follows. The growth of the surface BC caused the surface temperature to rise due to the increasing heating effect of BC. The continuous growth of the surface temperature made it easier for the PBL height to develop, which led to the lift of the inversion layer in the PBL and the larger atmospheric environment capacity. Ultimately, it is conducive to the diffusion of the near surface pollutants, thus helping reduce their concentrations, which offsets the increasing tendency of O3 and add to the decreasing trend of PM2.5. This phenomenon is the most remarkable in summer, with the fastest increasing rate of temperature (0.8 °C⋅a-1) and O3 (3.9 µg⋅m-3⋅a-1) during 2015-2019 (excluding 2020 to erase the great effect of COVID-19 lockdown on emissions).


Assuntos
Poluentes Atmosféricos , Poluição do Ar , COVID-19 , Poluentes Atmosféricos/análise , Poluição do Ar/análise , Poluição do Ar/prevenção & controle , COVID-19/epidemiologia , COVID-19/prevenção & controle , Carbono , China , Controle de Doenças Transmissíveis , Monitoramento Ambiental , Humanos , Material Particulado/análise , Rios , Fuligem
17.
J Fish Dis ; 45(10): 1429-1437, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35930453

RESUMO

Micropterus salmoides rhabdovirus (MSRV) is one of the common pathogens in the largemouth bass industry, which can cause lethal diseases in juvenile fish and enormous economic losses. To establish effective means to prevent MSRV infection, the pcDNA3.1-G plasmid containing the MSRV glycoprotein gene was successfully constructed and intramuscularly injected into the largemouth bass to evaluate the immune responses and protective effects in our study. As the results showed, the serum antibody levels of the fish vaccinated with different doses of pcDNA3.1-G were significantly higher compared with the control groups (PBS and pcDNA3.1). Meanwhile, the immune parameters (acid phosphatase and alkaline phosphatase) were also significantly up-regulated. Several immune-related genes (IgM, IL-8, IL-12p40 and CD40) were expressed in the pcDNA3.1-G groups at higher levels than in the control groups, which indicated that strong immune responses were induced. Besides, the survival percentages of fish in the control groups (PBS and pcDNA3.1) and pcDNA3.1-G groups (2.5, 5, 10 and 20 µg/fish) at 14 days after challenge experiment with MSRV were 0%, 0%, 6.1%, 15.2%, 29.0% and 48.5% respectively. This study indicated that pcDNA3.1-G was a prospective DNA vaccine candidate against MSRV-induced mortality.


Assuntos
Bass , Doenças dos Peixes , Rhabdoviridae , Vacinas de DNA , Animais , Estudos Prospectivos , Rhabdoviridae/genética
18.
Zhongguo Dang Dai Er Ke Za Zhi ; 24(8): 899-907, 2022 Aug 15.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-36036129

RESUMO

OBJECTIVES: To develop the birth weight curve of twin neonates with a gestational age of 25-40 weeks, and to investigate the regional differences of the birth weight curve. METHODS: A total of 11 maternal and child health care hospitals with more than 7 000 neonates delivered annually were selected in 11 cities of China (Haikou, Guangzhou, Liuzhou, Guilin, Quanzhou, Shenzhen, Chongqing, Chengdu, Changsha, Ningbo, and Lianyungang), and all live twin neonates delivered in the 11 hospitals from January 1, 2017 to December 31, 2020 were enrolled for the development of birth weight curves. RESULTS: A total of 17 256 twin neonates with a gestational age of 25-40 weeks from the 11 cities were included in the study. The reference values of the 3rd-97th percentiles of birth weight of twin neonates for the total of the 11 cities and for each of the 11 cities in China were established, and the birth weight percentile curves were drawn. The birth weight curve level of twin neonates in Liuzhou was lower than the average level of the 11 cities; the birth weight curve level of twin neonates in Ningbo was higher than the average level of the 11 cities; the birth weight curve level of twin neonates in Lianyungang was obviously higher than the average level of the 11 cities; the birth weight curve level of twin neonates in other 8 cities was almost the same as the average level of the 11 cities. CONCLUSIONS: The reference values of the 3rd-97th percentiles of birth weight of twin neonates for the total of the 11 cities and for each of the 11 cities are developed, which can be used as a reference for evaluating the intrauterine growth of twin neonates in the region. The level of intrauterine growth of twin neonates in some cities is different from the average level of the 11 cities of China.


Assuntos
Gêmeos , Peso ao Nascer , Criança , China , Cidades , Idade Gestacional , Humanos , Lactente , Recém-Nascido
19.
Infect Immun ; : e0024722, 2022 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-36040155

RESUMO

The oral epithelial barrier acts as both a physical barrier to the abundant oral microbiome and a sentry for the immune system that, in health, constrains the accumulation of the polymicrobial plaque biofilm. The immune homeostasis during gingivitis that is largely protective becomes dysregulated, unproductive, and destructive to gingival tissue as periodontal disease progresses to periodontitis. The progression to periodontitis is associated with the dysbiosis of the oral microbiome, with increasing prevalences and abundances of periodontal pathogens such as Treponema denticola. Despite the association of T. denticola with a chronic inflammatory disease, relatively little is known about gingival epithelial cell responses to T. denticola infection. Here, we characterized the transcriptome of gingival keratinocytes following T. denticola challenge and identified interleukin-36γ (IL-36γ) as the most differentially expressed cytokine. IL-36γ expression is regulated by p65 NF-κB and the activation of both the Jun N-terminal protein kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) pathways downstream of Toll-like receptor 2 (TLR2). Finally, we demonstrate for the first time that mitogen- and stress-activated kinase 1 (MSK1) contributes to IL-36γ expression and may link the activation of MAPK and NF-κB signaling. These findings suggest that the interactions of T. denticola with the gingival epithelium lead to elevated IL-36γ expression, which may be a critical inducer and amplifier of gingival inflammation and subsequent alveolar bone loss.

20.
Front Immunol ; 13: 966098, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35979361

RESUMO

COVID-19, caused by SARS-CoV-2, has resulted in hundreds of millions of infections and millions of deaths worldwide. Preliminary results exhibited excellent efficacy of SARS-CoV-2 vaccine in preventing hospitalization and severe disease. However, data on inactivated vaccine-induced immune responses of naturally infected patients are limited. Here, we characterized SARS-CoV-2 RBD-specific IgG (anti-S-RBD IgG) and neutralizing antibodies (NAbs) against SARS-CoV-2 wild type and variants of concerns (VOCs), as well as RBD-specific IgG-secreting B cells and antigen-specific T cells respectively in 51 SARS-CoV-2 recovered subjects and 63 healthy individuals. In SARS-CoV-2 recovered patients, a single dose vaccine is sufficient to reactivate robust anti-S-RBD IgG and NAbs. The neutralizing capacity against VOCs increased significantly post-vaccination no matter healthy individuals or SARS-CoV-2 recovered patients. In addition, RBD-specific IgG-secreting B cells in SARS-CoV-2 recovered patients were significantly higher than that in healthy vaccine recipients. After the vaccine booster, the frequencies of specific IFN-γ+ CD4+ T cell, IL-2+ CD4+ T cell, and TNF-α+ CD4+ T cell responses were significantly increased in SARS-CoV-2 recovered patients. Our data highlighted the safety and utility of SARS-CoV-2 inactivated vaccine and demonstrated that robust humoral and cellular immune response can be reactivated by one-dose inactivated vaccine in SARS-CoV-2 recovered patients.


Assuntos
COVID-19 , Vacinas Virais , Anticorpos Neutralizantes , Anticorpos Antivirais , COVID-19/prevenção & controle , Vacinas contra COVID-19 , Humanos , Imunidade Celular , Imunoglobulina G , SARS-CoV-2 , Vacinação , Vacinas de Produtos Inativados
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