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1.
Food Chem ; 442: 138615, 2024 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-38309242

RESUMO

Rice gluten, as the hydrophobic protein, exhibits restricted application value in hydrophilic food, which may be enhanced through interaction with soybean 11S globulin, characterized by favorable functional properties. This study aims at revealing their interaction mechanism via multi-spectroscopy and molecular dynamics simulation. The formation and structural change of rice glutelin-soybean 11S globulin complexes were detected using fluorescence, ultra-violet and circular dichroism spectra. The addition of 11S globulin increased the contents of α-helix, ß-turn and random coil, but decreased ß-sheet content, and the change in secondary structure was correlated with particle size. Moreover, exposure of hydrophobic groups and formation of disulfide bonds occurred in the complexes. Molecular dynamics simulation verified these experimental results through analyses of root mean square deviation and fluctuation, hydrogen bond, secondary structure, and binding free energy analysis. This study contributes to expounding the interaction mechanism of protein and protein from the molecular level.


Assuntos
Globulinas , Oryza , Glutens/química , Oryza/metabolismo , Simulação de Dinâmica Molecular , Espectrometria de Fluorescência , Globulinas/química , Simulação de Acoplamento Molecular
2.
Zhongguo Zhen Jiu ; 43(8): 932-6, 2023 Aug 12.
Artigo em Chinês | MEDLINE | ID: mdl-37577891

RESUMO

OBJECTIVE: To observe the clinical effect of repeated transcranial acupuncture combined with electroacupuncture on flat descending sudden deafness. METHODS: A total of 80 patients with flat descending sudden deafness were randomly divided into an acupuncture and medication group (39 cases, 1 case dropped off) and a western medication group (39 cases, 1 case dropped off). Patients in the western medication group were treated with glucocorticoid + batroxobin + ginkgo leaf preparation. On the basis of the western medication group, patients in the acupuncture and medication group were treated with acupuncture at Baihui (GV 20), Ningshen, Yunting area, Mastoid 1 point, Mastoid 2 point, Fengchi (GB 20), Gongxue, Tinghui (GB 2), etc., repeated transcranial acupuncture was applied at Baihui (GV 20), Ningshen and Yunting area; Mastoid 1 point and Mastoid 2 point, Fengchi (GB 20) and Gongxue of the affected side were connected to the electroacupuncture instrument, continuous wave, 2 Hz in frequency. The needle was retained for 30 min, once a day, and rest for 1 d after 6 d of continuous treatment. All patients were treated for 3 weeks. The average hearing threshold, tinnitus handicap inventory (THI) score, dizziness handicap inventory (DHI) score and speech discrimination test score were compared between the two groups before and after treatment, and the clinical effect was evaluated. RESULTS: After treatment, the average hearing threshold, THI and DHI scores of the two groups were lower than those before treatment (P<0.05), and above indexes in the acupuncture and medication group were lower than those in the western medication group (P<0.05). The speech discrimination test scores of the two groups were higher than those before treatment (P<0.05), and the score in the acupuncture and medication group was higher than that in the western medication group (P<0.05). The total effective rate was 87.2% (34/39) in the acupuncture and medication group, which was higher than 74.4% (29/39) in the western medication group (P<0.05). CONCLUSION: Repeated transcranial acupuncture combined with electroacupuncture can improve the hearing level of patients with flat descending sudden deafness, relieve tinnitus and vertigo symptoms.


Assuntos
Terapia por Acupuntura , Eletroacupuntura , Perda Auditiva Súbita , Zumbido , Humanos , Zumbido/terapia , Perda Auditiva Súbita/terapia , Resultado do Tratamento , Pontos de Acupuntura
3.
ACS Appl Mater Interfaces ; 15(10): 13644-13655, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36861749

RESUMO

Biofouling and corrosion of underwater equipment induced by marine organisms have become major issues in the marine industry. The superior corrosion resistance of Fe-based amorphous coatings makes them suitable for marine applications; however, they have a poor antifouling ability. In this work, a hydrogel-anchored amorphous (HAM) coating with satisfactory antifouling and anticorrosion performance is designed, utilizing an interfacial engineering strategy involving micropatterning, surface hydroxylation, and a dopamine intermediate layer to increase the adhesion strength between the hydrogel layer and the amorphous coating. The as-obtained HAM coating exhibits exceptional antifouling properties, achieving 99.8% resistance to algae, 100% resistance to mussels, and excellent biocorrosion resistance against Pseudomonas aeruginosa. Antifouling and anticorrosion performance of the HAM coating was also explored by conducting a marine field test in the East China Sea, and no signs of corrosion and fouling are observed after 1 month of immersion. It is revealed that the outstanding antifouling properties stem from the killing-resisting-camouflaging trinity that resists organism attachment across different length scales, and the excellent anticorrosion performance originates from the remarkable barrier of the amorphous coating against Cl- ion diffusion and microbe-induced biocorrosion. This work presents a novel methodology for designing marine protective coating with excellent antifouling and anticorrosion properties.

4.
Zhongguo Zhen Jiu ; 43(3): 261-4, 2023 Mar 12.
Artigo em Chinês | MEDLINE | ID: mdl-36858385

RESUMO

To introduce the clinical experience of professor SUN Shen-tian in treatment of Tourette's syndrome (TS) with acupuncture. TS is a psychosomatic disease and the core pathogenesis refers to blood deficiency producing internal wind. The disease is located in the heart and liver. Acupoints are selected according to the functional orientation of the cerebral cortex. The extrapyramidal system area is preferred for tic disorder, and the emotional area is for behavioral disorder. The treatment focuses on regulating the mind by multiple methods, including manual needling technique used the transcranial repeated acupuncture, and regulating the mind by taking multiple acupoints, Baihui (GV 20), Neiguan (PC 6), Shenmen (HT 7) and Dazhong (KI 4) are stimulated. For abdominal twitching and mental symptoms of TS children, the first and third abdominal areas are selected. The target symptoms (biao) are treated specially by local acupoints, the combination of the starting and ending acupoints of the affected meridian, or the acupoints of the meridians with same name. The modified chaihu longgu muli decoction and siwu decoction are prescribed to sooth liver, nourish blood and soothe wind. In association with the characteristics and target symptoms of TS, the sequential therapy is used with filiform needling, intradermal needling, Chinese herbal medication and psychotherapy.


Assuntos
Terapia por Acupuntura , Meridianos , Síndrome de Tourette , Criança , Humanos , Fígado , Psicoterapia
5.
Zhongguo Zhen Jiu ; 43(2): 197-202, 2023 Feb 12.
Artigo em Chinês | MEDLINE | ID: mdl-36808515

RESUMO

The paper introduces GAO Wei-bin's clinical experience in acupuncture treatment for neurogenic bladder. In association with the etiology, the location and types of neurogenic bladder and in accordance with nerve anatomy and meridian differentiation, the acupoints are selected accurately in treatment. Four acupoint prescriptions are allocated. For frequent urination and urinary incontinence, the foot-motor-sensory area of scalp acupuncture, Shenshu (BL 23) and Huiyang (BL 35) are used. For all kinds of urine retention, especially the patients who are not suitable for acupuncture at the lumbar region, Zhongji (CV 3), Qugu (CV 2), Henggu (KI 11) and Dahe (KI 12) are selected. For all kinds of urine retention, Zhongliao (BL 33) and Ciliao (BL 32) are applicable. For the patients with both dysuria and urinary incontinence, Zhongliao (BL 33), Ciliao (BL 32) and Huiyang (BL 35) are chosen. In treatment of neurogenic bladder, both biao (root causes) and ben (primary symptoms) are considered, as well as the accompanying symptoms; and electroacupuncture is combined accordingly. During the delivery of acupuncture, the sites where the acupoints located are detected and palpated so as to rationally control the depth of needle insertion and the operation of reinforcing and reducing needling techniques.


Assuntos
Terapia por Acupuntura , Eletroacupuntura , Meridianos , Bexiga Urinaria Neurogênica , Incontinência Urinária , Retenção Urinária , Humanos , Bexiga Urinaria Neurogênica/etiologia , Terapia por Acupuntura/efeitos adversos , Pontos de Acupuntura
6.
Food Chem ; 408: 135230, 2023 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-36549163

RESUMO

The work aimed to assess the antioxidant ability and obtain a new antioxidant peptide from rice bran protein. Rice bran protein was hydrolyzed by Alcalase, Neutral, Pepsin, Chymotrypsin, and Trypsin, separately. Trypsin hydrolysate (T-RBPH) showed high Fe2+ chelating activity (IC50, 2.271 ± 0.007 mg/mL), DPPH and hydroxyl radical scavenging ability (IC50, 0.191 ± 0.006 and 1.038 ± 0.034 mg/mL). Moreover, T-RBPH could alleviate the H2O2-induced oxidative damage in Caco-2. The T-RBPH was purified and identified by UF, GF, FPLC, and LC-MS/MS. Finally, 9-amino acid peptide-AFDEGPWPK with low molecular weight (1045.48 Da), high antioxidant activity, good safety, and solubility was screened by in silico method and chemical oxidation determination, and its interaction with Keap1 was also demonstrated. The ORAC and DPPH radical scavenging ability of AFDEGPWPK were 44.16 ± 0.79 and 28.38 ± 0.14 µmol TE/mM. Moreover, the Molecular docking and Western blot (WB) results showed that AFDEGPWPK could enter the binding pocket in the Kelch domain and activate Keap1/Nrf2/HO-1 pathway.


Assuntos
Antioxidantes , Oryza , Humanos , Antioxidantes/farmacologia , Antioxidantes/química , Hidrolisados de Proteína/química , Oryza/química , Proteína 1 Associada a ECH Semelhante a Kelch/metabolismo , Cromatografia Líquida , Tripsina/metabolismo , Simulação de Acoplamento Molecular , Peróxido de Hidrogênio/metabolismo , Células CACO-2 , Espectrometria de Massas em Tandem , Fator 2 Relacionado a NF-E2/metabolismo , Peptídeos/química
7.
Food Res Int ; 162(Pt B): 112108, 2022 12.
Artigo em Inglês | MEDLINE | ID: mdl-36461408

RESUMO

Broken rice is an important by-product during milling process of rice, which is rich in protein. To increase the value of by-products and search for effective antioxidants, the antioxidant peptides from broken rice protein hydrolysate were separated and identified by ultrafiltration, gel filtration chromatography, fast protein liquid chromatography, and LC-MS/MS in this study. These identified peptides were further screened using a combined in silico and in vitro method and their antioxidant mechanism was explored by Western blot and molecular docking analysis. Ninety-eight peptides were obtained after antioxidant activity-oriented isolation and four novel peptides, SGDWSDIGGR, DFGSEILPR, GEPFPSDPKKQLQ, and GEKGGIPIGIGK, with excellent solubility, safety, and antioxidant activity were synthesized. Among these, SGDWSDIGGR showed good antioxidant activities in the extracellular assay (41.57 µmol TE/g and 29.41 % in ORAC and DPPH assay, respectively.), and it possessed a protective effect against H2O2-injured oxidative stress in 2BS cells in a dose-dependent manner. Furthermore, Western blot and molecular docking results showed that SGDWSDIGGR achieves antioxidant ability by occupying the Nrf2-binding site, activating the Keap1-Nrf2 signaling pathway, and upregulating the expression of antioxidant enzymes. This study extends the rice industry chain and provides insights into the selection and mechanisms research of antioxidant peptides.


Assuntos
Oryza , Hidrolisados de Proteína , Hidrolisados de Proteína/farmacologia , Antioxidantes/farmacologia , Fator 2 Relacionado a NF-E2 , Proteína 1 Associada a ECH Semelhante a Kelch , Peróxido de Hidrogênio , Cromatografia Líquida , Simulação de Acoplamento Molecular , Espectrometria de Massas em Tandem , Peptídeos/farmacologia
8.
Zhongguo Zhen Jiu ; 42(11): 1285-9, 2022 Nov 12.
Artigo em Chinês | MEDLINE | ID: mdl-36397227

RESUMO

This paper introduces GAO Wei-bin's academic thought in treatment of medulla oblongata paralysis with acupuncture. Through analyzing the etiologies and locations of medulla oblongata paralysis, in accordance with "selecting the nearby acupoints of the affected area", the acupoints are selected from the nape region, the nape acupuncture therapy and the corresponding new points are developed. Based on the human anatomy of the nape region, the anatomic structures of new points (e.g. Gongxue, Tunyan-1, Tunyan-2, Fayin, Zhiqiang and Tiyan) and their effect mechanism are explained. The treatment principle, "distinguishing the symptoms from the root causes, mutual treatment for both symptoms and root causes", is proposed, and the importance of electric stimulation of nape acupuncture is suggested in treatment of medulla oblongata paralysis.


Assuntos
Terapia por Acupuntura , Acupuntura , Paralisia Bulbar Progressiva , Humanos , Pontos de Acupuntura , Paralisia Bulbar Progressiva/terapia , Bulbo , Paralisia
9.
Zhongguo Zhen Jiu ; 42(5): 511-4, 2022 May 12.
Artigo em Chinês | MEDLINE | ID: mdl-35543941

RESUMO

OBJECTIVE: To compare the clinical effect between head acupuncture combined with exercise therapy and conventional acupuncture for nonspecific low back pain. METHODS: A total of 64 patients with nonspecific low back pain were randomized into an observation group (32 cases, 2 cases dropped off) and a control group (32 cases, 2 cases dropped off). In the control group, conventional acupuncture was applied at Jiaji (EX-B 2) of L1 to L3, ashi point, Shenshu (BL 23), Dachangshu (BL 25), Yaoyangguan (GV 3) and Weizhong (BL 40). The observation group was treated with head acupuncture combined with exercise therapy, head acupuncture was applied at foot-motor-sensory area on the healthy side and Cuanzhu (BL 2), Tongziliao (GB 1) on the affected side, and McKenzie therapy was performed during retention. The needles were retained for 40 min, once a day, continuous treatment for 6 days with the interval of 1 day, 14 days were required in the two groups. Before and after treatment, the pain visual analogue scale (VAS) score, Oswestry disability index (ODI) score and infrared thermography temperature of pain area in the low back were compared in the two groups. RESULTS: Compared before treatment, the VAS and ODI scores after treatment were decreased in the two groups (P<0.01), and those in the observation group were lower than the control group (P<0.01). Compared before treatment, the infrared thermography temperature of pain area in the low back after treatment was increased in the two groups (P<0.01), and that in the observation group was higher than the control group (P<0.01). CONCLUSION: Head acupuncture combined with exercise therapy could relieve pain, improve dysfunction and increase the local temperature of pain area in patients with nonspecific low back pain, and its curative effect is better than conventional acupuncture.


Assuntos
Terapia por Acupuntura , Acupuntura , Dor Lombar , Pontos de Acupuntura , Terapia por Exercício , Humanos , Dor Lombar/terapia , Resultado do Tratamento
10.
Zhongguo Zhen Jiu ; 42(1): 75-8, 2022 Jan 12.
Artigo em Chinês | MEDLINE | ID: mdl-35025161

RESUMO

To summarize SUN Shen-tian's treatment ideas and clinical features. SUN applies meridian syndrome differentiation to the diagnosis and treatment of diseases; advocates that prevention and treatment of diseases should be regulated mind firstly; applies transcranial repetitive acupuncture combined modern cerebral cortex function positioning; emphasizes the application of multiple acupuncture methods and manipulation, and includes the meridian penetrating needling method, the flat needling and penetrating needling method, and the stagnant needle lifting method, pays attention to the importance of achieving qi and manipulation for the effect.


Assuntos
Terapia por Acupuntura , Acupuntura , Meridianos , Moxibustão , Pontos de Acupuntura
11.
Zhongguo Zhen Jiu ; 41(2): 189-91, 2021 Feb 12.
Artigo em Chinês | MEDLINE | ID: mdl-33788468

RESUMO

Professor SUN Shen-tian's academic thought and clinical experience of acupuncture for peripheral facial paralysis are summarized. According to the different stages of facial paralysis, professor SUN 's treatment plan has its own emphasis. In the acute stage, professor SUN emphasizes the importance of standardized western medicine treatment, and proposes early acupuncture treatment. The treatment mainly focuses on dispersing wind and cold, promoting blood circulation and dredging collaterals. In the recovery stage, professor SUN emphasizes the method of "pulling the sticking needle", and applies repeated transcranial acupuncture according to the functional orientation of cerebral cortex. In the sequelae stage, professor SUN emphasizes the value of regulating the mind. In the course of treatment, professor SUN highly values the use of flexible acupoint selection, repeated transcranial acupuncture, "pulling the sticking needle" method, acupoint pressing and giant-needle method, combined with electroacupuncture and targeted injection. In addition, based on the clinical experience, professor SUN summarizes the common methods to estimate the prognosis of peripheral facial paralysis.


Assuntos
Terapia por Acupuntura , Acupuntura , Paralisia Facial , Moxibustão , Pontos de Acupuntura , Paralisia Facial/terapia , Humanos
12.
Food Chem ; 346: 128880, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33418415

RESUMO

The variations in flavor substances across the different stages of fermented soybean whey tofu (FSWT) production were analyzed by headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) combined with principal component analysis (PCA). The results revealed 24 representative flavor compounds in the samples across all production stages. After heating, the signal intensity of hexanal, 1-octen-3-ol, heptanal, and (E)-2-hexenol, which are unpleasant flavor substances found in raw soymilk, weakened, whereas those of some aroma substances increased. Furthermore, fermented flavor compounds, namely, 2-heptanone, 2-pentylfuran, pentanal, and 2,3-butanedione, were produced after the addition of fermented soybean whey as a coagulant. A PCA based on the signal intensity of the detected volatile compounds revealed effective differentiation of samples from different stages into comparatively independent spaces. These results showed that the flavor fingerprints of the samples from different stages of FSWT production can be successfully built using HS-GC-IMS and PCA based on the detected volatile compounds.


Assuntos
/química , Alimentos de Soja/análise , Compostos Orgânicos Voláteis/análise , Cromatografia Gasosa , Fermentação , Espectrometria de Mobilidade Iônica , Análise de Componente Principal , Compostos Orgânicos Voláteis/química
13.
Zhongguo Zhen Jiu ; 27(11): 810-2, 2007 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-18085142

RESUMO

OBJECTIVE: To observe therapeutic effect of Yuanluo Tongjing needling method for treatment of mild cognitive impairment and search for an effective therapy of TCM for mild cognitive impairment. METHODS: Sixty cases were randomly divided into an observation group and a control group, 30 cases in each group. The observation group were treated by Yuanluo Tongjing needling method with Baihui (GV 20), Danzhong (CV 17), Guanyuan (CV 4), Shenmen (HT 7), etc. selected, and the control group by oral administration of Aricept, 2.5 mg. Changes of scores of all indexes in clinical memory scale and memory quotient (MQ) before and after treatment were investigated in the two groups, and clinical therapeutic effects were assessed by using MQ increasing values. RESULTS: After treatment for 30 days, the scores of memory quotient, direction memory, picture free memory, re-cognition of no-significant picture forms and associated memory of human picture characteristics significantly increased (P < 0.01, P < 0.05) in the observation group; and all the indexes except associated memory in the control group significantly changed (P < 0.05), with no significant difference between the two groups (P > 0.05). The good rate for memory effect was 80.0% in the observation group and 76.7% in the control group with no significant difference between the two groups (P > 0.05). CONCLUSION: Yuanluo Tongjing needling method can improve memory ability of the patient of mild cognitive impairment.


Assuntos
Terapia por Acupuntura/métodos , Transtornos Cognitivos/terapia , Medicina Tradicional Chinesa , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
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