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1.
J Oleo Sci ; 68(9): 863-871, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31484902

RESUMO

The variations in average particle size, zeta potential, free fatty acids (FFA) release rate, and the bioavailability of menthol under in vitro simulated digestion conditions of peppermint oil nanoemulsion were investigated. 3D confocal laser scanning microscopy and Cryo-scanning electron microscopy were used to observe the microstructure characteristics of peppermint oil nanoemulsion, which indicated that soybean protein was completely adsorbed at the oil-water interface of the nanoemulsion and presented a core shell structure. And the results indicated that FFA release rate and menthol bioavailability of peppermint oil nanoemulsion prepared by using high-pressure homogenization were much higher. In the simulated gastric digestion phase, the average particle size and the zeta potential of the nanoemulsion increased, and droplet polymerization appeared. After the simulated intestinal, the interfacial protein of nanoemulsion was hydrolyzed, and the oil droplets were digested, which resulted in the decreased particle size and increased absolute value of zeta potential.

2.
Int J Mol Med ; 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31485603

RESUMO

Although a number of experimental models have been developed for liver research, each has its own advantages and disadvantages. The present study attempted to develop a simple and effective 3­dimensional mouse liver microsphere tissue culture (LMTC) model in vitro for the analysis of hepatic functions. Hepatic characteristics and potential applications of this model were compared with that of mouse model in vivo and mouse primary hepatocytes in vitro. Using freshly­perfused mouse liver tissue passed through 80­mesh sift strainer (sift80), it was demonstrated that under the optimal culture conditions, the sift80 microsphere tissue cultured in 2% bovine calf serum medium remained viable with marked proliferating cell nuclear antigen and anti­Myc proto­oncogene protein expression, exhibited normal hepatic functions including indocyanine green (ICG) uptake/release and periodic acid­Schiff staining, and expressed hepatocyte­specific genes for up to 2 weeks. The microsphere tissue was responsive to bone morphogenic protein 9 (BMP9) stimulation leading to upregulation of downstream targets of BMP9 signaling. Furthermore, 3 commonly­used liver­damaging drugs were indicated to effectively inhibit hepatic ICG uptake, and induce the expression of hepatotoxicity­associated genes. Therefore, this simplified LMTC model may be a useful in vitro tissue culture model to investigate drug­induced liver injury and metabolism, and hepatocyte­based cell singling.

3.
Sensors (Basel) ; 19(18)2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31505879

RESUMO

This paper proposed an optimal spectral resolution for diagnosing cadmium-lead (Cd-Pb) cross contamination with different pollution levels based on the hyperspectral reflectance of rice canopy. Feature bands were sequentially selected by two-way analysis of variance (ANOVA2) and random forests from the high-dimensional hyperspectral data after preprocessing. Then Support Vector Machine (SVM) was applied to diagnose the pollution levels using different feature bands combination with different spectral resolutions and cross validation was conducted to evaluate the distinguishing accuracies. Finally, the optimal spectral resolution could be determined by comparing the diagnosing accuracies of the optimal feature bands combination in each spectral resolution. In the experiments, the hyperspectral reflectance data of rice canopy with ten different spectral resolutions was captured, covering 16 pretreatments of Cd and Pb pollution. The experimental results showed the optimal spectral resolution was 9 nm with the highest average accuracy of 0.71 and relatively standard deviation of 0.07 for diagnosing the categories and levels of Cd-Pb cross contamination. The useful exploration provided an evidence for optimal spectral resolution selection to reduce the cost of heavy metal pollution diagnose.

4.
Hepatology ; 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31509261

RESUMO

We found that lncRNA PDIA3P1 (protein disulfide isomerase family A member 3 pseudogene 1) was up-regulated in multiple cancer types and upon treatment with DNA-damaging chemotherapeutic agents, like doxorubicin (Dox). Higher PDIA3P1 level was associated with poorer recurrence-free survival of human hepatocellular carcinoma (HCC). Both gain- and loss-of-function studies revealed that PDIA3P1 protected cancer cells from Dox-induced apoptosis and allowed tumor xenografts to grow faster and to be more resistant to Dox treatment. Mechanistically, miR-125a/b and miR-124 suppressed the expression of tumor necrosis factor receptor-associated factor 6 (TRAF6), but PDIA3P1 bound to miR-125a/b/miR-124 and relieved their repression on TRAF6, leading to activation of the nuclear factor-K B (NF-K B) pathway. Consistently, the effect of PDIA3P1 inhibition in promoting Dox-triggered apoptosis was antagonized by silencing the inhibitor of K Bα (IK Bαor overexpressing TRAF6. Administration of BAY 11-7085, an NF-K B inhibitor, attenuated PDIA3P1-induced resistance to Dox treatment in mouse xenografts. Moreover, up-regulation of PDIA3P1 was significantly correlated with elevation of TRAF6, phosphorylated p65, or NF-K B downstream anti-apoptosis genes in human HCC tissues. These data indicate that enhanced PDIA3P1 expression may confer chemoresistance by acting as a microRNA (miRNA) sponge to increase TRAF6 expression and augment NF-K B signaling. Subsequent investigations into the mechanisms of PDIA3P1 up-regulation revealed that human homologue of mRNA transport mutant 4 (hMTR4), which promotes RNA degradation, could bind to PDIA3P1, and this interaction was disrupted by Dox treatment. Overexpression of hMTR4 attenuated Dox-induced elevation of PDIA3P1, whereas silencing hMTR4 increased PDIA3P1 level, suggesting that Dox may up-regulate PDIA3P1 by abrogating the hMTR4-mediated PDIA3P1 degradation CONCLUSION: There exists a novel hMTR4-PDIA3P1-miR-125/124-TRAF6 regulatory axis that promotes NF-K B signaling and chemoresistance, which may be exploited for anticancer therapy.

6.
Anal Chem ; 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31509397

RESUMO

Effective extension of mass spectrometry-based proteomics to single cells remains challenging. Herein we combined microfluidic nanodroplet technology with tandem mass tag (TMT) isobaric labeling to significantly improve analysis throughput and proteome coverage for single mammalian cells. Isobaric labeling facilitated multiplex analysis of single cell-sized protein quantities to a depth of ~1,600 proteins with median CV of 10.9% and correlation coefficient of 0.98. To demonstrate in-depth high throughput single cell analysis, the platform was applied to measure protein expression in 72 single cells from three murine cell populations (epithelial, immune, and endothelial cells) in <2 days instrument time with over 2,300 proteins identified. Principal component analysis grouped the single cells into three distinct populations based on protein expression with each population characterized by well-known cell-type specific markers. Our platform enables high throughput and unbiased characterization of single cell heterogeneity at the proteome level.

7.
Int J Cancer ; 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31509622

RESUMO

The N6 -Methyladenosine (m6 A) modification plays an important role in many biological processes, especially tumor development. However, little is still known about how it affects colorectal cancer (CRC) carcinogenesis. Here we first systematically investigate the association of variants related to m6 A modification with the CRC risk in 1,062 CRC cases and 2,184 controls by using our exome-wide association data and followed by two replication sets including 7,341 CRC cases and 7,902 controls. The variant rs8100241 located in ANKLE1 was significantly associated with CRC risk (OR = 0.88, 95% CI= 0.84-0.92, P = 4.85×10-8 ) in 8,403 cases and 10,086 controls. This variant was previously identified to be associated with the susceptibility of breast cancer with BRCA1 mutation triple negative breast cancer. Further functional analysis indicated that overexpression of the rs8100241[A] allele significantly increased the ANKLE1 m6 A level and facilitated the ANKLE1 protein expression compared to that of rs8100241[G] allele. We further found the ANKLE1 m6 A modification was catalysed by the "writer" complex (METTL3, METTL14 or WTAP) and recognized by the "reader" YTHDF1. Mechanistically, we found that the ANKLE1 functions as a potential tumor suppressor that inhibits cell proliferation and facilitates the genomic stability. An elevated frequency of micronucleated cells, increased cell proliferation and colony formation ability were observed when ANKLE1 knockdown. Our study illustrated that the germline missense variant can increase CRC risk by influencing ANKLE1 m6 A level, highlighting a clinical potential of variants-associated m6 A modification as a risk marker for CRC prevention. This article is protected by copyright. All rights reserved.

8.
J Exp Clin Cancer Res ; 38(1): 333, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31370861

RESUMO

BACKGROUND: Cathepsin L (CTSL) is a cysteine protease known to have important roles in regulating cancer cellular resistance to chemotherapy. However mechanism underlying which regulates CTSL-mediated drug resistance remain largely unknown. METHODS: We used NSCLC cell lines: A549, A549/TAX (paclitaxel-resistant), A549/DDP (cisplatin-resistant), H460 and PC9 cells, to evaluate CTSL and drug resistance changes. Tumor specimens from 53 patients with NSCLC and Xenograft models was also utilized to explore the regulatory relationship of CTSL, TGF-ß, Egr-1 and CREB. RESULTS: TGF-ß and smad3 were overexpressed only in A549/TAX cells, silencing TGF-ß or smad3 in A549/TAX cells decreased the expression of CTSL and enhanced their sensitivity to paclitaxel. Smad3 binds to the Smad-binding-element(SBE) of the CTSL promoter, resulting in increased activity of the CTSL promoter and subsequent CTSL. Egr-1 and CREB were overexpressed only in A549/DDP cells, and silencing Egr-1 or CREB reduced the expression of CTSL and increased cisplatin cytotoxicity. CREB could affect the activity of the CTSL promoter by binding to it. And the potential regulatory factors of CTSL were consistent in vivo and in human lung cancer. These different regulatory mechanisms of CTSL-mediated drug resistance exist in two other NSCLC cell lines. CONCLUSION: CTSL-mediated drug resistance to paclitaxel and cisplatin may be modulated by different mechanisms. The results of our study identified different mechanisms regulating CTSL-mediated drug resistance and identified smad3 as a novel regulator of CTSL.

9.
BMJ Open ; 9(7): e024409, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31371283

RESUMO

OBJECTIVE: Tuberculosis (TB) remains a major deadly threat in mainland China. Early warning and advanced response systems play a central role in addressing such a wide-ranging threat. The purpose of this study is to establish a new hybrid model combining a seasonal autoregressive integrated moving average (SARIMA) model and a non-linear autoregressive neural network with exogenous input (NARNNX) model to understand the future epidemiological patterns of TB morbidity. METHODS: We develop a SARIMA-NARNNX hybrid model for forecasting future levels of TB incidence based on data containing 255 observations from January 1997 to March 2018 in mainland China, and the ultimate simulating and forecasting performances were compared with the basic SARIMA, non-linear autoregressive neural network (NARNN) and error-trend-seasonal (ETS) approaches, as well as the SARIMA-generalised regression neural network (GRNN) and SARIMA-NARNN hybrid techniques. RESULTS: In terms of the root mean square error, mean absolute error, mean error rate and mean absolute percentage error, the identified best-fitting SARIMA-NARNNX combined model with 17 hidden neurons and 4 feedback delays had smaller values in both in-sample simulating scheme and the out-of-sample forecasting scheme than the preferred single SARIMA(2,1,3)(0,1,1)12 model, a NARNN with 19 hidden neurons and 6 feedback delays and ETS(M,A,A), and the best-performing SARIMA-GRNN and SARIMA-NARNN models with 32 hidden neurons and 6 feedback delays. Every year, there was an obvious high-risk season for the notified TB cases in March and April. Importantly, the epidemic levels of TB from 2006 to 2017 trended slightly downward. According to the projection results from 2018 to 2025, TB incidence will continue to drop by 3.002% annually but will remain high. CONCLUSIONS: The new SARIMA-NARNNX combined model visibly outperforms the other methods. This hybrid model should be used for forecasting the long-term epidemic patterns of TB, and it may serve as a beneficial and effective tool for controlling this disease.

10.
PLoS Pathog ; 15(8): e1008002, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31404116

RESUMO

The galectin 3 binding protein (LGALS3BP, also known as 90K) is a ubiquitous multifunctional secreted glycoprotein originally identified in cancer progression. It remains unclear how 90K functions in innate immunity during viral infections. In this study, we found that viral infections resulted in elevated levels of 90K. Further studies demonstrated that 90K expression suppressed virus replication by inducing IFN and pro-inflammatory cytokine production. Upon investigating the mechanisms behind this event, we found that 90K functions as a scaffold/adaptor protein to interact with TRAF6, TRAF3, TAK1 and TBK1. Furthermore, 90K enhanced TRAF6 and TRAF3 ubiquitination and served as a specific ubiquitination substrate of TRAF6, leading to transcription factor NF-κB, IRF3 and IRF7 translocation from the cytoplasm to the nucleus. Conclusions: 90K is a virus-induced protein capable of binding with the TRAF6 and TRAF3 complex, leading to IFN and pro-inflammatory production.

11.
Ann Hepatol ; 18(5): 770-776, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31422029

RESUMO

INTRODUCTION AND OBJECTIVES: Acute liver failure (ALF) is a severe disease which is associated with a high mortality rate. As mild hypothermia has been shown to have protective effects on the brain, this study aimed to determine whether it also provides protection to the liver in rats with ALF and to explore its underlying mechanism. MATERIALS AND METHODS: In total, 72 rats were divided into 3 groups: control group (CG, treated with normal saline), normothermia group (NG, treated with d-galactosamine and lipopolysaccharide; d-GalN/LPS), and mild hypothermia group (MHG, treated with d-GalN/LPS and kept in a state of mild hypothermia, defined as an anal temperature of 32-35°C). The rats were examined at 4, 8, and 12h after treatment. RESULTS: Mild hypothermia treatment significantly reduced serum alanine transaminase and aspartate transaminase levels and improved the liver condition of rats with d-GalN/LPS-induced ALF at 12h. Serum tumor necrosis factor-alpha levels were significantly lower in the MHG than in the NG at 4h, but no significant differences were observed in the interleukin-10 levels between the NG and MHG at any time. The serum and hepatic levels of high mobility group box 1 were significantly lower in the MHG than in the NG at 8 and 12h. The protein expression levels of cytochrome C and cleaved-caspase 3 in hepatic tissues were significantly lower in the MHG than in the NG at 8h. CONCLUSION: Mild hypothermia improved the liver conditions of rats with ALF via its anti-inflammatory and anti-apoptotic effects.

12.
Medicine (Baltimore) ; 98(32): e16806, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31393411

RESUMO

BACKGROUND: Non-steroidal anti-inflammatory drugs (NSAIDs) especially aspirin has been gained increasing attention due to its potential therapy against to lung cancer. Previous investigations have showed different findings in this issue. We studied the safety profile and efficacy of NSAIDs in treating lung cancer. METHOD: Embase, Pubmed, and Cochrane Library databases were searched from January 2011 to February 2019. We identified the studies meeting a priori inclusion criteria and it also conducted a secondary review. This meta-analysis of 5 prospective studies was launched to evaluate the effect of NSAIDs for patients with lung cancer on the hazard risk (HR). We used the Random-Effect Model to assess pooled HR and between-study heterogeneity. Application of subgroup analysis, meta-regression, as well as sensitivity analysis was to pinpoint the exact sources of the observed heterogeneity. RESULTS: 5 Prospective Cohorts Studies, including 6017 patients with lung cancer were recruited in the final meta-analysis. In general, using of NSAIDs especially aspirin is not associated with mortality of lung cancer: pooled hazard ratio (HR) of 0.88 [95% confidence intervals (CI): 0.73-1.05] with low heterogeneity (Q = 6.95; I = 42.4%, P = .139). Egger (P = .665) and Begg (P = 1.000) test also showed little trial error in this meta-analysis. CONCLUSION: NSAIDs did not increase the risk of mortality in patients with lung cancer.


Assuntos
Anti-Inflamatórios não Esteroides/uso terapêutico , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/mortalidade , Anti-Inflamatórios não Esteroides/administração & dosagem , Anti-Inflamatórios não Esteroides/efeitos adversos , Aspirina/uso terapêutico , Humanos , Estudos Prospectivos
13.
Aging (Albany NY) ; 11(16): 6312-6335, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31434796

RESUMO

Limited studies have focused on developing prognostic models with trans-omics biomarkers for early-stage lung adenocarcinoma (LUAD). We performed integrative analysis of clinical information, DNA methylation, and gene expression data using 825 early-stage LUAD patients from 5 cohorts. Ranger algorithm was used to screen prognosis-associated biomarkers, which were confirmed with a validation phase. Clinical and biomarker information was fused using an iCluster plus algorithm, which significantly distinguished patients into high- and low-mortality risk groups (Pdiscovery = 0.01 and Pvalidation = 2.71×10-3). Further, potential functional DNA methylation-gene expression-overall survival pathways were evaluated by causal mediation analysis. The effect of DNA methylation level on LUAD survival was significantly mediated through gene expression level. By adding DNA methylation and gene expression biomarkers to a model of only clinical data, the AUCs of the trans-omics model improved by 18.3% (to 87.2%) and 16.4% (to 85.3%) in discovery and validation phases, respectively. Further, concordance index of the nomogram was 0.81 and 0.77 in discovery and validation phases, respectively. Based on systematic review of published literatures, our model was superior to all existing models for early-stage LUAD. In summary, our trans-omics model may help physicians accurately identify patients with high mortality risk.

14.
J Environ Radioact ; 208-209: 106008, 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31419761

RESUMO

Channelization significantly affects soil erosion in river floodplains. The object of this study was to use 137Cs as a tracer to determine the 137Cs inventory and derived soil erosion rates under various land use types in a catchment on channelized river floodplain in the lower reaches of Yangtze River, China. Sampling was carried out to establish a137Cs reference inventory in a 70-year old paddy field located on the shoulder-slope of a local hill. The mean reference inventory of 137Cs was 1275 Bq m-2, whereas the 137Cs inventory within the catchment ranged from 284 to 1150 Bq m-2 and the soil erosion rates from -33.3 to -2.4 t ha-1 yr-1, respectively. The dominated land use of paddy in cultivated soils contributed relative low soil erosion. Bamboo and castanea mollissima were preferential for local land uses in uncultivated soils in comparison with woodland and Pinnus massoniana. The rates of soil erosion rates in old tea garden were higher than that in new tea garden. Overall, severe soil erosion and no deposition in the entire catchment occurred in the entire catchment due to the human-induced channelization in the 1970s. Our results suggest that restricting farmland being returned to tea plantations, thereby maintaining the current land use types would reduce soil erosion in river floodplain in the future.

15.
Genet Test Mol Biomarkers ; 23(8): 533-556, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31373850

RESUMO

Background: To determine whether vitamin D receptor (VDR) gene polymorphisms are correlated with susceptibility to diabetic vascular complications. Methods: We included all eligible studies, and used Stata12.0 to calculate the pooled results. Results: Eight thousand eleven diabetic patients and 1635 normal controls from 27 studies were included. Our results showed that there was no correlation between VDR gene TaqI variants and diabetic nephropathy (DN) or diabetic retinopathy (DR) susceptibility. In comparison with diabetic patients without DN, there was a link between the VDR gene ApaI variant and DN susceptibility under allelic model (p = 0.029) in all populations. In addition, the VDR gene BsmI variant correlated with DN under both dominant (p = 0.005) and allelic (p = 0.003) models in Asian populations. The VDR gene FokI variant was also correlated with DN susceptibility under the recessive model (p = 0.027) in the Asian subgroup. In comparison with diabetic patients without DR, we identified a link between the VDR gene ApaI variant and DR susceptibility under the dominant model (p = 0.034) in all populations. Also, the VDR gene FokI variant was correlated with DR under the recessive (p = 0.016), the allelic (p = 0.001), and the dominant (p < 0.001) models in all populations. When compared with healthy controls, the VDR gene BsmI variant was associated with DR under the additive (p = 0.014), the allelic (p = 0.033), and the dominant (p < 0.001) models in Indian populations. Conclusions: The VDR gene BsmI, ApaI, and FokI gene variants are associated with DN and DR susceptibility. No association was found between the VDR gene TaqI gene variants and diabetic vascular complications.

16.
Arch Virol ; 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31428915

RESUMO

Current antiviral therapies against hepatitis B virus (HBV) infections, such as treatment with nucleos(t)ide analogs (NAs) and interferon alpha, can significantly lower HBV DNA titers, eventually to undetectable levels. However, it is still difficult to completely eliminate the stable template of HBV, the covalently closed circular DNA (cccDNA), and this contributes to viral rebound when treatment is discontinued. HBV pregenomic RNA (pgRNA), which was recently found to be present in the enveloped mature HBV viral particle in blood, is tentatively regarded, with still accumulating clinical evidence, as a novel bona fide virological marker reflecting the amount and status of cccDNA when serum HBV DNA becomes undetectable. HBV pgRNA and DNA share almost identical sequences, and it is therefore difficult to differentiate pgRNA from viral DNA using normal PCR methods. To exclude interference from viral DNA, methods for measuring pgRNA usually require a selective DNA degradation step, which is complicated and time-consuming and also compromises the accuracy of detection. In this study, we developed a simplified quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay with improved accuracy achieved by probing the polyA tail of pgRNA. Using clinical serum samples, we observed that not all patients share the same 3' sequence, suggesting slight differences between HBV strains in the way they end transcription. We then designed and evaluated a universal primer and probe set for distinguishing HBV pgRNA from HBV DNA. Our results demonstrated that a one-step qRT-PCR assay could selectively amplify HBV pgRNA from a mixture of HBV RNA and DNA, which is valuable for clinical applications.

17.
Future Oncol ; 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31424272

RESUMO

Aim: The purpose of this study was to investigate the predictive power of the systemic immune inflammation index (SII) based on neutrophil (N), platelet (P) and lymphocyte (L) on the clinical outcomes of patients with SCLC. Patients & methods: Blood samples of 228 patients were obtained 1 week before treatment to measure the SII (SII = P × N/L). Overall survival (OS) and progression-free survival (PFS) were estimated using the Kaplan-Meier curves and Cox regression models. Results: Higher SII was associated with poorer OS (p < 0.001) and poorer PFS (p < 0.001). Multivariable analyses further revealed SII as an independent prognostic factor for OS (p < 0.001) and PFS (p < 0.001). Conclusion: Pretreatment SII was a valuable prognostic factor for PFS and OS in SCLC patients.

18.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(4): 1131-1137, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31418368

RESUMO

OBJECTIVE: To explore the clinical and pathologic features as well as prognosis of systemic EBV-positive T-cell lymphoma in children. METHODS: The clinical data including clinical manifestation, pathologic changes and treatment in 16 patients with children's systemic EBV-positive T-cell lymphoma were analyzed retrospectively, and follow-up of patients were carried out. RESULTS: The 16 cases included 12 males and 4  females with median age of 3.3 years old. It was demonstrated that the clinical and pathological features of the children's systemic EBV-positive T-cell lymphoma were as followed fever, hepatosplenomegaly, cytopenia, lymphadenopathy, and hemophagocytosis in bone marrow or organ. Histologically, the structures of lymph node was normal, partially or completely destoryed. The paracortical zone was expanded with prominent infiltration of small to medium-sized atypical lymphocytes. The major immunophenotypic characteristics were as follows: (1) Almost all biopsies exhibited prominent T cell proliferation. (2) CD3 was expressed in 16 patients (100%, 16/16), CD4 in 5 patients (31.3%, 5/16),CD5 in 13 patients (81.3%, 13/16),CD7 was expressed in 11 patients (68.8%, 11/16),CD8 in 15 patients (93.8%, 15/16),CD4 and CD8 were expressed in 5 patients (31.3%, 5/16),CD4 and CD8 double-negative in patients (6.3%, 1/16),16 patients were CD56 negative (100%, 16/16). (3) TCR gene cloning rearrangement in 16 patients (93.8%, 15/16). (4) EBV-EBER was expressed in 16 patients (100%, 16/16). 11 out of 16 cases died, 1 cese failed to be followed up, 1 case relapsed,and 3 cases survived, reseptively. The media survival time was 4 months. CONCLUSION: Systemic EBV-positive T-cell lymphoma predominantly occurred in childhood and early teen-age, and lacks specific clinic features, usually combined with hemophagocytic syndrome. The confirmed diagnosis requires comprehensive analysis of clinical manifestation, pathomorphology, immunohistochemical detection, EBV-EBER insite hybridization, and TCR gene test. The overall prognosis of the disease is poor and the fatality rate is high.


Assuntos
Infecções por Vírus Epstein-Barr , Linfoma de Células T , Adolescente , Pré-Escolar , Infecções por Vírus Epstein-Barr/complicações , Feminino , Herpesvirus Humano 4 , Humanos , Linfoma de Células T/etiologia , Masculino , Estudos Retrospectivos , Linfócitos T
19.
Thyroid ; 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31452441

RESUMO

BACKGROUND: Co-occurrence of TERT promoter (TERTp) mutations with BRAF/RAS mutations is associated with significantly more aggressive thyroid cancer. TERTp mutations are hypothesized to generate de novo binding sites for ETS transcription factors, which are themselves activated by BRAF/RAS-stimulated MEK-ERK activity. To date, a detailed study of this mechanism has been limited to only a few cancer types, and we hypothesized that ETS factors involved in TERTp activation could vary between different cancers. METHODOLOGY: Here we sought to identify ETS factor(s) required for TERTp activation in thyroid cancer, using a combination of in silico analyses of TCGA data, and experimentation using in vitro thyroid cell models analysed by qRT-PCR, immunoprecipitation (IP), chromatin IP (ChIP) and gene reporter assays. RESULTS: We found that ETV5 was abundantly expressed in papillary thyroid cancers (PTCs) from the TCGA dataset, and in thyroid cancer cell-lines TPC1, SW1736 and C643. Furthermore, ETV5 demonstrated specific binding affinity for the -124bp(T) TERTp allele, and stimulated TERT transcription in thyroid cancer cells. In contrast GABPA, which is critical for TERTp activation in several other malignancies, was functionally redundant in our thyroid cancer models. We also found that ETV5 functionally cooperates with the transcription factor FOXE1 to further enhance TERTp activity, a mechanism that may at least partially explain why FOXE1 represents a significant genetic determinant of thyroid cancer risk. CONCLUSIONS: ETS factors that activate mutant TERTp vary between cancer types, and here we show for the first time that ETV5 demonstrates mutant allele-specific affinity for TERTp in thyroid cancer, a property that has previously only been attributable to GABPA.

20.
Sci Total Environ ; 696: 133982, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31470327

RESUMO

Phthalates (PAEs) are in a group of artificial chemicals with potential adverse effects to human health and they can be frequently detected in environmental matrices due to its extensive usage. However, seasonal patterns of concentrations in atmosphere and risks posed by PAEs in airborne PM2.5 to Chinese population have not been well characterized. During the period of November 2015 to March 2017, samples of fine particulate matter (PM2.5) were collected in four cities of Guangzhou, Shanghai, Beijing and Harbin, which are major metropolitan areas of various latitudes of China. Concentrations of fourteen PAEs in airborne PM2.5 were quantified using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). Estimated daily intakes (EDIs), hazard quotients (HQs) and hazard index (HI) were calculated. Lifetime average daily doses (LADD) and incremental lifetime cancer risks (ILCR) of di(2-ethylhexyl) phthalate (DEHP) for four age groups, which divide with infant, toddler, adolescent and adult, by inhalation route were evaluated. Dimethyl phthalate (DMP), Diethyl phthalate (DEP), Di-n-butyl phthalate (DBP), and DEHP were the four major PAEs contaminants in these PM2.5 samples. The sum concentrations of DMP, DEP, DBP and DEHP in Guangzhou, Shanghai, Beijing and Harbin ranged from 32.5-76.1, 10.1-101, 8.02-107 and 13.5-622 ng/m3, with mean concentrations of 59.1, 50.8, 43.8 and 136 ng/m3, respectively. The concentration of total PAEs in PM2.5 from higher latitudes city (Harbin) was higher than those from lower latitudes cities (Guangzhou and Shanghai). Total concentrations of PAEs were significantly higher during warmer seasons than those during colder seasons among the four cities. Although the EDIs, HQs, and HI for all age groups were less than the threshold set by the U.S. Environmental Protection Agency (US EPA) and European Food Safety Authority (EFSA), the highest values of 70-years ILCR from Shanghai and Harbin were 1.2 × 10-6 and 1.3 × 10-6, which were slightly beyond the acceptable level of 10-6. These findings reveal that the cancer risks of DEHP bound to PM2.5 in these two cites should be of particular concern.

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