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1.
Fish Shellfish Immunol ; 98: 564-573, 2020 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-32001354

RESUMO

A relatively large repertoire of type I interferon (IFN) genes is apparent in rainbow trout/Atlantic salmon, that includes six different IFN subgroups (IFNa-IFNf) belonging to the three known type I IFN groups (1-3) in bony fish. Whether this is true for other salmonids, and how the various type I subgroups evolved in teleost fish was studied using the extensive genomic resources available for fish. This confirmed that salmonids, at least the Salmoninae, indeed have a complex (in terms of IFN subgroups present) and large (number of genes) IFN repertoire relative to other teleost fish. This is in part a consequence of the salmonid 4 R WGD that duplicated the growth hormone (GH) locus in which type I IFNs are generally located. Divergence of the IFN genes at the two GH loci was apparent but was not seen in common carp, a species that also underwent an independent 4 R WGD. However, expansion of IFN gene number can be found at the CD79b locus of some perciform fish (both freshwater and marine), with expansion of the IFNd gene repertoire. Curiously the primordial gene order of GH-IFNc-IFNb-IFNa-IFNe is largely retained in many teleost lineages and likely reflects the tandem duplications that are taking place to increase IFN gene number. With respect to the evolution of the IFN subgroups, a complex acquisition and/or loss has occurred in different teleost lineages, with complete loss of IFN genes at the GH or CD79b locus in some species, and reduction to a single IFN subgroup in others. It becomes clear that there are many variations to be discovered regarding the mechanisms by which fish elicit protective (antiviral) immune responses.

2.
Aging (Albany NY) ; 122020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-32023553

RESUMO

The treatment for intervertebral disc degeneration (IDD) has drawn great attention and recent studies have revealed that the p38 MAPK pathway is a potential therapeutic target for delaying the degeneration of intervertebral discs. In this study, we analyzed a nature-derived protein tyrosine kinase inhibitor, Genistein, and its function in delaying IDD in rats both in vitro and in vivo via the p38 MAPK pathway. Nucleus pulposus cells treated with Genistein showed better function compared with untreated cells. Further study revealed that Genistein could play a protective role in IDD by inhibiting phosphorylation of p38, consequently inhibiting the p38 pathway-mediated inflammatory response. The rat IDD model also demonstrated that Genistein could effectively delay the degeneration of intervertebral disc tissue. The current study reveals new biological functions of Genistein, further demonstrates the effects of the p38 MAPK pathway on intervertebral disc degeneration, and deepens our understanding of the treatment and prevention of IDD.

3.
Curr Eye Res ; 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32027190

RESUMO

Purpose: To compare the differences of higher order aberrations (HOAs) after laser subepithelial keratomileusis (LASEK) between two different laser platforms.Methods: One hundred and seven eyes of 107 patients were included in this study. Fifty-six eyes underwent LASEK with the Triple-A profile (an ablation profile of the MEL 90 excimer laser) and fifty-one eyes underwent LASEK with the aspheric (Aberration Smart Ablation, ASA) profile. Uncorrected distance visual acuity (UDVA), corrected distance visual acuity (CDVA), corneal topography, and ocular aberrations were measured before and 6 months postoperatively.Results: In ASA group, the values of horizontal trefoil,vertical coma, horizontal coma, spherical aberrations, and total HOAs increased significantly after surgery (all P<0.05). There was no significant differences in the vertical trefoil between the preoperative and 6-month postoperative period. In Triple-A group, there were no differences in vertical trefoil and horizontal trefoil values between the preoperative and 6 months postoperative periods. Compared with the preoperative values, vertical coma, horizontal coma, spherical aberrations, and total HOAs were significantly increased 6 months after surgery (all P<0.05). Compared to the Triple-A group, higher horizontal trefoil and horizontal coma were introduced in the ASA group at 6 months postoperatively.Conclusion: The Triple-A ablation profile of the MEL 90 Excimer laser at a 500 Hz pulse rate was an efficient method to correct myopia for mild to moderate myopia, compared with the aspheric ablation model, fewer horizontal trefoil and horizontal coma were induced at six months after LASEK.

4.
J Cell Physiol ; 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-32003015

RESUMO

C-type lectin domain family 11 member A (Clec11a), also known as stem cell growth factor (SCGF), C-type lectin superfamily member 3 (CLECSF3), or osteolectin was initially identified as a growth factor for hematopoietic progenitor cells. The human Clec11a gene encodes a polypeptide of 323 amino acids with characteristics of a secreted glycoprotein encompassing two integrin-binding motifs, RGD (Arg-Gly-Asp) and LDT (Leu-Asp-Thr), a putative leucine zipper domain, and a functional C-type lectin domain. It regulates hematopoietic differentiation and homeostasis and exhibits a protective effect against severe malarial anemia and lipotoxicity. Furthermore, Clec11a promotes the differentiation of mesenchymal progenitors into mature osteoblasts in vitro and plays an important role in the maintenance of adult skeleton age-related bone loss and fracture repair. Receptor ligand binding results in activation of downstream signaling cascades including glycogen synthase kinase 3 (GSK3), ß-catenin, and Wnt, resulting in the expression of osteoblast-related gene transcripts including Alp, Runx2, Lef1, and Axin2. In addition, Clec11a is also associated with the development of several cancers, including leukemia, multiple myeloma, and gastrointestinal tract tumors. To date, however, the mechanisms governing transcription regulation of the Clec11a gene are not known and remain to be uncovered. Understanding the function and mechanism of action of Clec11a will pave the way for the development of Clec11a as a novel therapeutic target for conditions such as cancer, anemia, and skeletal diseases.

5.
Semin Cancer Biol ; 2020 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-31962173

RESUMO

Cancer has thwarted as a major health problem affecting the global population. With an alarming increase in the patient population suffering from diverse varieties of cancers, the global demographic data predicts sharp escalation in the number of cancer patients. This can be expected to reach 420 million cases by 2025. Among the diverse types of cancers, the most frequently diagnosed cancers are the breast, colorectal, prostate and lung cancer. From years, conventional treatment approaches like surgery, chemotherapy and radiation therapy have been practiced. In the past few years, increasing research on molecular level diagnosis and treatment of cancers have significantly changed the realm of cancer treatment. Lately, uses of advanced chemotherapy and immunotherapy like treatments have gained significant progress in the cancer therapy, but these approaches have several limitations on their safety and toxicity. This has generated lot of momentum for the evolution of new drug delivery approaches for the effective delivery of anticancer therapeutics, which may improve the pharmacokinetic and pharmacodynamic effect of the drugs along with significant reduction in the side effects. In this regard, the protein-based nano-medicines have gained wider attention in the management of cancer. Proteins are organic macromolecules essential, for life and have quite well explored in developing the nano-carriers. Furthermore, it provides passive or active tumour cell targeted delivery, by using protein based nanovesicles or virus like structures, antibody drug conjugates, viral particles, etc. Moreover, by utilizing various formulation strategies, both the animal and plant derived proteins can be converted to produce self-assembled virus like nano-metric structures with high efficiency in targeting the metastatic cancer cells. Therefore, the present review extensively discusses the applications of protein-based nano-medicine with special emphasis on intracellular delivery/drug targeting ability for anticancer drugs.

6.
Biomaterials ; 230: 119617, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31771859

RESUMO

Tissue engineering is a promising strategy for the repair of large-scale bone defects, in which scaffolds and growth factors are two critical issues influencing the efficacy of bone regeneration. Unfortunately, the broad application of growth factors is limited by their poor stability in the scaffolds. In the present study, the strictly controlled expression of human bone morphogenetic protein-4 (hBMP-4) in the presence of doxycycline is achieved by adding an hBMP-4 gene fragment into a non-viral artificial restructuring plasmid vector (pSTAR) to form the pSTAR-hBMP-4 plasmid (phBMP-4). Furthermore, the controlled release of phBMP-4 is obtained with an electroactive tissue engineering scaffold, generated by combining a triblock copolymer of poly(l-lactic acid)-block-aniline pentamer-block-poly(l-lactic acid) (PLA-AP) with poly(lactic-co-glycolic acid)/hydroxyapatite (PLGA/HA). This PLGA/HA/PLA-AP/phBMP-4 composite scaffold, with controlled gene release and Dox-regulated gene expression upon electrical stimulation, operating synergistically, exhibits an improved cell proliferation ability, enhanced osteogenesis differentiation in vitro, and effective bone healing in vivo in a rabbit radial defect model. Taking these results together, the proposed smart PLGA/HA/PLA-AP/phBMP-4 scaffold lays a solid theoretical and experimental basis for future applications of such multi-functional materials in bone tissue engineering to help patients in need.

7.
Am J Physiol Gastrointest Liver Physiol ; 318(1): G10-G22, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31433213

RESUMO

Gastric cancer (GC) is one of the most common cancers in the world and remains a heavy burden of health worldwide. Adenylate cyclase 3 (ADCY3) is a widely expressed membrane-associated protein in human tissues and has been identified to be a new molecular target of GC. Long noncoding RNAs have a substantial influence on tumorigenesis and progression of tumors by binding to microRNAs. Therefore, this study is to clarify the mechanism by which LINC00319 sponges micro RNA-335-5p (miR-335-5p) to influence the development of GC. Initially, microarray analysis identified GC-related differentially expressed LINC00319 and ADCY3 for this study. The interaction was confirmed that LINC00319 interacted with miR-335-5p to regulate ADCY3. Next, SGC-7901 cells presenting with the lowest LINC00319 expression and the highest miR-335-5p expression were transfected with LINC00319, miR-335-5p inhibitor, or ADCY3 vector to examine their roles in growth and metastasis of GC cells, which was further ascertained by in vivo experiments. LINC00319 was upregulated and miR-335-5p was downregulated in GC cells. LINC00319 overexpression, miR-335-5p inhibitor, or ADCY3 overexpression was shown to significantly elevate the expression of cyclin-dependent kinase 4 and metastasis associated 1, decrease that of growth arrest-specific 1, and promote tumor growth and metastasis by increasing proliferation and migration and reducing cell apoptosis. Importantly, it was found that overexpressed miR-335-5p exerted its tumor suppressive role in GC through downregulating ADCY3. Collectively, LINC00319 expedited growth and metastasis of GC by upregulating miR-335-5p-mediated ADCY3.NEW & NOTEWORTHY This study is carried out based on in vivo and in vitro studies in mice and gastric cancer (GC) cells with the aim of clarifying the role of LINC00319 on GC growth and metastasis, which associated with micro RNA-335-5p-mediated adenylate cyclase 3. Altogether, we identified LINC00319 to be a potential therapy to treat GC.

8.
Adv Healthc Mater ; 9(2): e1901176, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31840437

RESUMO

The development of a universal influenza vaccine is an ideal strategy to eliminate public health threats from influenza epidemics and pandemics. This ultimate goal is restricted by the low immunogenicity of conserved influenza epitopes. Layered protein nanoparticles composed of well-designed conserved influenza structures have shown improved immunogenicity with new physical and biochemical features. Herein, structure-stabilized influenza matrix protein 2 ectodomain (M2e) and M2e-neuraminidase fusion (M2e-NA) recombinant proteins are generated and M2e protein nanoparticles and double-layered M2e-NA protein nanoparticles are produced by ethanol desolvation and chemical crosslinking. Immunizations with these protein nanoparticles induce immune protection against different viruses of homologous and heterosubtypic NA in mice. Double-layered M2e-NA protein nanoparticles induce higher levels of humoral and cellular responses compared with their comprising protein mixture or M2e nanoparticles. Strong cytotoxic T cell responses are induced in the layered M2e-NA protein nanoparticle groups. Antibody responses contribute to the heterosubtypic NA immune protection. The protective immunity is long lasting. These results demonstrate that double-layered protein nanoparticles containing structure-stabilized M2e and NA can be developed into a universal influenza vaccine or a synergistic component of such vaccines. Layered protein nanoparticles can be a general vaccine platform for different pathogens.

9.
Food Chem Toxicol ; 135: 110921, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31669597

RESUMO

Determining chemical carcinogenicity in the early stages of drug discovery is fundamentally important to prevent the adverse effect of carcinogens on human health. There has been a recent surge of interest in developing computational approaches to predict chemical carcinogenicity. However, the predictive power of many existing approaches is limited, and there is plenty of room for improvement. Here, we develop a new deep learning architecture, termed CapsCarcino, to distinguish between carcinogens and noncarcinogens. CapsCarcino is constructed based on a dynamic routing algorithm that requires less data, extracts more comprehensive information, and does not require feature selection. We find that CapsCarcino provides a significantly improved predictive and generalization ability over, and outperforms five other machine learning models. Specifically, the best model of CapsCarcino achieves an accuracy of 85.0% on an external validation dataset. In addition, we discover that the enhanced predictive capability of CapsCarcino over that of the other methods is robust and can be achieved using sparse datasets. Training on merely 20% of the dataset, CapsCarcino performs comparably to the other methods based on the full training dataset. Further mechanism analysis indicates that CapsCarcino could efficiently learn the characteristics of carcinogens even if structural alerts are insufficiently represented. The results indicate that CapsCarcino should be helpful for carcinogen risk assessment.

10.
Dev Comp Immunol ; 104: 103571, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31837379

RESUMO

To elucidate the immunity-protecting role of the interferon-ß promoter stimulator-1 (ScIPS-1) in barbel chub Squaliobarbus curriculus, the full-length cDNA of ScIPS-1 was cloned and expression levels in response to stimulation were investigated. In addition, the function of ScIPS-1 and its domains were analyzed. The full-length cDNA of ScIPS-1 is 2524 bp and encodes 601 aa. The N-terminal caspase activation and recruitment domain, central proline-rich domain, C-terminal transmembrane domain, C2HC-zinc finger, and Cwf21 domains were identified. The mRNA level of ScIPS-1 was the highest in the kidney, whereas the highest protein level was observed in the liver. The ScIPS-1 expressions were significantly up-regulated after lipopolysaccharide and poly I:C treatment. The ScIPS-1 protein level was up-regulated at 12 h in the head kidney and was up-regulated at 12 h and then down-regulated from 12 to 48 h in the liver after grass carp reovirus (GCRV) infection. The CiIFN and CiMx transcription levels were significantly enhanced in pEGFP-C1-IPS-1 and pcDNA3.1-ΔCwf21 overexpressing cells after GCRV infection. The results indicate that ScIPS-1 may function in the immune response against pathogens and provide a basis for achieving resistance to diseases in fish breeding.

11.
Adv Exp Med Biol ; 1179: 17-37, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31741332

RESUMO

Chronic hepatitis B virus (HBV) infection remains to be a serious threat to public health and is associated with many liver diseases including chronic hepatitis B (CHB), liver cirrhosis, and hepatocellular carcinoma. Although nucleos(t)ide analogues (NA) and pegylated interferon-α (Peg-IFNα) have been confirmed to be efficient in inhibiting HBV replication, it is difficult to eradicate HBV and achieve the clinical cure of CHB. Therefore, long-term therapy has been recommended to CHB treatment under the current antiviral therapy. In this context, the new antiviral therapy targeting one or multiple critical steps of viral life cycle may be an alternative approach in future. In the last decade, the functional receptor [sodium-taurocholate cotransporting polypeptide (NTCP)] of HBV entry into hepatocytes has been discovered, and the immature nucleocapsids containing the non- or partially reverse-transcribed pregenomic RNA, the nucleocapsids containing double-strand linear DNA (dslDNA), and the empty particles devoid of any HBV nucleic acid have been found to be released into circulation, which have supplemented the life cycle of HBV. The understanding of HBV life cycle may offer a new instruction for searching the potential antiviral targets, and the new viral markers used to monitor the efficacy of antiviral therapy for CHB patients in the future.


Assuntos
Antivirais , Vírus da Hepatite B , Hepatite B Crônica , Antivirais/farmacologia , Vírus da Hepatite B/efeitos dos fármacos , Vírus da Hepatite B/genética , Vírus da Hepatite B/crescimento & desenvolvimento , Hepatite B Crônica/virologia , Hepatócitos/virologia , Humanos , Interferon-alfa/farmacologia , Internalização do Vírus/efeitos dos fármacos , Replicação Viral/efeitos dos fármacos
12.
Free Radic Biol Med ; 147: 262-270, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31883468

RESUMO

OBJECTIVES: The degeneration of intervertebral discs (IVD) is a risk factor for chronic low back pain. Anti-inflammation therapy could alleviate IVD degeneration. IL-10 is an important anti-inflammatory cytokine. However, the effect of IL-10 on IVD has not been fully revealed. The current study is to reveal the effect of IL-10 on IVD and its underlying mechanism. METHODS: IL-1ß was used to induce the degeneration of nucleus pulposus cells (NPCs). mRNA expression level was determined by qPCR. Protein expression level was determined by western blotting. Methylene blue was used to determined the expression of aggrecan. Immunocytochemical staining was used to determined the expression of collagen II. A rat caudal IVD degeneration model was established and used to evaluate the effect of IL-10 on IVD in vivo. RESULTS: IL10 could alleviated NPC degeneration in both morphology and extracellular matrix. IL-10 could increase the mRNA expression of Collagen II, Sox-9, but decrease the mRNA expression of IL-1ß, TNFα and Collagen X. IL-10 could also increase the protein level of Collagen II and aggrecan, but decrease that of Collagen X. Western blotting futher revealed the mechanism of the positive effect of IL-10 on IVD. IL-10 reduces phosphorylation level of p38 MAPK effectively. Rat caudal IVD degeneration model futher confirmed the positive effect of IL-10 on IVD degeneration and its mechanism in vivo. CONCLUSION: The current study demonstrates that exogenous IL-10 treatment can induce an anti-inflammatory response and inhibit p38 MAPK activation to delay IVD degeneration.

13.
Nutr Metab (Lond) ; 16: 79, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31788011

RESUMO

Background: Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease and has become a public health concern worldwide. The hallmark of NAFLD is hepatic steatosis. Therefore, there is an urgent need to develop new therapeutic strategies that are efficacious and have minimal side effects in hepatic steatosis and NAFLD treatment. The present study aimed to investigate the effect of dietary supplement of curcumin on high-fat diet (HFD)-induced hepatic steatosis and the underlying mechanism. Methods: ApoE-/- mice were fed a normal diet, high-fat diet (HFD) or HFD supplemented with curcumin (0.1% w/w) for 16 weeks. Body and liver weight, blood biochemical.parameters, and liver lipids were measured. Intestinal permeability, hepatic steatosis and mRNA and protein expressions of TLR4-related inflammatory signaling molecule were analyzed. Results: The administration of curcumin significantly prevented HFD-induced body weight gain and reduced liver weight. Curcumin attenuated hepatic steatosis along with improved serum lipid profile. Moreover, curcumin up-regulated the expression of intestinal tight junction protein zonula occluden-1 and occludin, which further improved gut barrier dysfunction and reduced circulating lipopolysaccharide levels. Curcumin also markedly down-regulated the protein expression of hepatic TLR4 and myeloid differentiation factor 88 (MyD88), inhibited p65 nuclear translocation and DNA binding activity of nuclear factor-κB (NF-κB) in the liver. In addition, the mRNA expression of hepatic tumour necrosis factor-α (TNF-α) and interleukin-1ß (IL-1ß) as well as the plasma levels of TNF-α and IL-1ß were also lowered by curcumin treatment. Conclusion: These results indicated that curcumin protects against HFD-induced hepatic steatosis by improving intestinal barrier function and reducing endotoxin and liver TLR4/NF-κB inflammation. The ability of curcumin to inhibit hepatic steatosis portrayed its potential as effective dietry intervention for NAFLD prevention.

14.
Oxid Med Cell Longev ; 2019: 5120275, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31885798

RESUMO

Melatonin, a neuroendocrine hormone secreted by the pineal body, has a positive effect on intervertebral disc degeneration. The present study is aimed at investigating the biological role of melatonin in intervertebral disc degeneration and its underlying mechanism. A human nucleus pulposus cell (NPC) line was exposed to melatonin at different concentrations. Cell proliferation was measured by CCK-8 assay. Cell cycle and apoptosis were analyzed by flow cytometry. Western blot was performed to measure the protein expression of indicated genes. A rabbit model of intervertebral disc degeneration was established to detect the role and mechanism of melatonin on intervertebral disc degeneration. Our study showed that melatonin promoted NPC viability and inhibited cell arrest. Furthermore, melatonin treatment led to the upregulation of collagen II and aggrecan and downregulation of collagen X. Moreover, melatonin significantly elevated the activity of the ERK signaling pathway. Inhibition of the ERK1/2 signals reversed the role of melatonin in the regulation of NPCs both in vitro and in vivo. Melatonin increased NPC viability through inhibition of cell cycle arrest and apoptosis. Moreover, melatonin promoted the secretion of functional factors influencing the nucleus pulposus cell physiology and retarded cell degeneration. Our results suggest that melatonin activated the ERK1/2 signaling pathway, thereby affecting the biological properties of the intervertebral disc degeneration.

15.
Emerg Microbes Infect ; 8(1): 367-376, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31851879

RESUMO

The dimorphic fungus Talaromyces marneffei (TM) is a common cause of HIV-associated opportunistic infections in Southeast Asia. Cotrimoxazole (CTX) inhibits folic acid synthesis which is important for the survival of many bacteria, protozoa, and fungi and has been used to prevent several opportunistic infections among HIV/AIDS patients. We question whether CTX is effective in preventing TM infection. To investigate this question, we conducted an 11-year (2005-2016) retrospective observational cohort study of all patients on the Chinese national antiretroviral therapy (ART) programme in Guangxi, a province with high HIV and TM burden in China. Survival analysis was conducted to investigate TM cumulative incidence, and Cox regression and propensity score matching (PSM) were used to evaluate the effect of CTX on TM incidence. Of the 3359 eligible individuals contributing 10,504.66 person-years of follow-up, 81.81% received CTX within 6 months after ART initiation, and 4.73% developed TM infection, contributing 15.14/1,000 person-year TM incidence rate. CTX patients had a significantly lower incidence of TM infection than non-CTX patients (4.11% vs. 7.53%; adjusted hazard ratio (aHR) = 0.50, 95% CI 0.35-0.73). CTX reduced TM incidence in all CD4+ cell subgroups (<50 cells/µL, 50-99 cells/µL, 100-199 cells/µL), with the highest reduction observed in patients with a baseline CD4+ cell count <50 cells/µL in both Cox regression and the PSM analyses. In conclusion, in addition to preventing other HIV-associated opportunistic infections, CTX prophylaxis has the potential to prevent TM infection in HIV/AIDS patients receiving ART.

16.
Zhongguo Zhong Yao Za Zhi ; 44(21): 4698-4703, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31872667

RESUMO

The aim of this paper was to investigate the protective effect of Shengdihuang Decoction(SDHD) on premature ovarian failure in rats and to explore its protective mechanism. Totally 48 adult female SD rats were randomly divided into six groups with 8 rats in each group: control group,model group,Bujiale group,SDHD high,medium and low dose group(12,6,3 g·kg-1). Rats were administered with Tripterygium Glycosides Tablets for 14 d to make model of premature ovarian failure except for control group. Rats were treated with corresponding medicines for 21 d after that. The oestrous cycle was observed,ovarian index and uterine index were detected,respectively. The variation in contents of E2,P,FSH,LH was detected with radioimmunoassay,the morphological changes of ovary and uterus were observed by HE staining,SOD activity and MDA content were detected in serum. The expression of ERα in ovarian and uterine tissues was detected by SABC,and the expression of ERα in uterus tissue was detected by Western blot. Compared with the model group,the index of the uterus and ovary in the high and middle dose group of Shengdihuang Decoction increased(P<0. 05),the level of serum E2 and P increased(P<0. 01,P<0. 05) and the level of LH decreased(P<0. 01). The number of ovarian follicles increased,the endometrium thickened,and the glands were developed,the activity of SOD was enhanced and the content of MDA decreased in serum,the expression of ERα in the follicle granulosa cells and the epithelial cells of the uterus increased,and the expression of ERα in the uterus increased. Shengdihuang Decoction could improve the morphology and function of the uterus and ovary,and relieve the premature failure of the ovary. The effect may be achieved by enhancing the antioxidant capacity of ovarian granulosa cells,restoring ovarian function,promoting serum estradiol and progesterone secretion,and increasing the expression of ER in uterine mucosal epithelial cells and ovarian granulosa cells.


Assuntos
Insuficiência Ovariana Primária , Animais , Antioxidantes , Feminino , Humanos , Ratos , Ratos Sprague-Dawley , Tripterygium
17.
Int J Biol Macromol ; 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31751714

RESUMO

To overcome the low mechanical strength and difficult bonding of hydrogels to bones which are the major limitations of hydrogels used in bone-regeneration, a new type of calcium polyphosphate incorporated into bioinspired alginate/polyacrylic acid (CPP/PAA-Alg) hybrid double network (DN) hydrogel with both high strength and enhanced osseointergration was prepared by a two-step polymerization with alginate and polyacrylic acid for bone regeneration. The morphology, mechanical properties, swelling, biocompatibility, osseointegration and osteogenic ability of this CPP/PAA-Alg DN hydrogel were investigated. The results show that CPP/PAA-Alg DN hydrogel with highly porous microstructure possesses high water absorption capacity and highly strength properties which meet the requirements of bone repairing. The results of in vitro studies revealed that the CPP/PAA-Alg DN hydrogels can support the spread of cells and promote the cell proliferation. Animal studies demonstrated that the CPP incorporated would enhance the osseointegration of DN hydrogel with host bone at an early stage after implantation to accelerate the regeneration of bone. This research may provide a new way to develop biocompatible biomaterials with high mechanical strength and good osseointegration to meet the needs of bone regeneration.

18.
Opt Express ; 27(22): 32666-32678, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31684475

RESUMO

An Al2O3:Mn4+, Mg2+ red emitting ceramic phosphor, which can be effectively excited by ultraviolet and blue light, was successfully synthesized via solid-state reaction in an oxygen and air atmosphere. The ceramic sintered in oxygen atmosphere has higher optical transmittance and stronger luminescence intensity than the ceramic sintered in the air, which is more suitable for LED application. Since the structure of α-Al2O3 is very simple, it is convenient to study the factors affecting the Mn4+ luminescence. The crystal-strength parameter Dq, Racah parameters B and C, and the nephelauxetic ratio ß1 were calculated to investigate the influence of crystal field strength and nephelauxetic effect on the emission of Mn4+ in the Al2O3 host. The ratio of Dq to B was 1.74, which was lower than 2.2. This indicated that the Mn4+ ions in the α-Al2O3 host were in a weak crystal field environment. Under the 395 nm and 460 nm excitations, quantum yields (QY) of the sample were measured to be 46% and 28.7%, respectively. The density measured by the Archimedes method was 3.61 g/cm3. The ceramic also showed an excellent thermal conductivity value, which was as high as 26.27 W·m-1·K-1@30 °C.

19.
Sci Rep ; 9(1): 16571, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31719624

RESUMO

The rock-climbing fish (Beaufortia kweichowensis) adheres to slippery, fouled surfaces and crawls both forward and backward in torrential streams. During locomotion, two suckers can be distinguished. Here, the general skeletal structure of the rock-climbing fish was determined using microtomography. Friction and adhesion were positively correlated, as were friction and fin ray angle. The unique adhesive locomotion system used by the rock-climbing fish was observed with a high speed camera. This system comprised two anisotropic suckers bearing two paired fins and two girdle muscles. A locomotion model was established based on these results. In this model, the fin states controlled the direction of motion using anisotropic friction, and alternate contractions of the girdle muscles provided propulsion during bidirectional crawling. This adhesive locomotion system was compared with other biological locomotion mechanisms. Based on these comparisons, we hypothesized that this novel system might represent an energy-saving solution for undulatory underwater vertical movement without detaching from the substrate.

20.
Adv Sci (Weinh) ; 6(21): 1901371, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31728286

RESUMO

Flexible material-based soft robots are widely used in various areas. In many situations, the suitable soft robots should be rapidly fabricated to complete the urgent tasks (such as rescue), so the facile fabricating methods of the multifunctional soft robots are still in urgent needs. In this work, the origami structure is employed to design vacuum-powered silicone rubber artificial muscles, which can perform multiple motions, including contraction, bending, twisting, and radial motions. Artificial muscles can be used for rapid reconfiguration of different soft robots, just like the "building bricks". Based on these artificial muscles, four soft robots with different functions, including an omnidirectional quadruped robot, a flexible gripper, a flexible wrist, and a pipe-climbing robot, are reconfigured to complete different tasks. The proposed origami artificial muscles offer a facile and rapid fabricating method of flexible material-based soft robots, and also greatly improve the utilization rate of flexible materials.

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