RESUMO
A total DNA extracted from Eucalyptus globlus, E. citriodora, or a subspecies of E. gunnii (Maruha Yu-kari) being used as an template, Polymerase Chain Reaction (PCR) was performed with 40 kinds of commercially available random primers (Operon, OPA-01-20 and OPB-01-20). After electrophoresis of PCR-products on agarose gel, we analyzed the band pattern formed by DNA-fragments with 0.5-3.0 kbp as molecular weight. As a remuneration for the examination in which forty 10-mer arbitrary oligonucleotides were tried to be used as primers in PCR for each species, we found that 9 primers (for E. globlus) leads polymorphism, 6 primers for E. citriodora, and 6 primers for Maruha Yu-kari.
Assuntos
DNA de Plantas/química , Polimorfismo Genético , Técnica de Amplificação ao Acaso de DNA Polimórfico , Árvores/genética , Sequência de Bases , Primers do DNA , DNA de Plantas/genética , Óperon , Sementes , Especificidade da Espécie , Árvores/classificaçãoRESUMO
A feline T-lymphoblastoid cell line susceptible to feline immunodeficiency viruses (FIV) was established and designated as Yu-1 cells. Yu-1 cells continued to grow over one year with more than 100 successive passages in the presence of human recombinant interleukin-2. Surface antigens of Yu-1 cells were feline Pan-T+, CD4+, and CD8-. Susceptibility of Yu-1 cells to FIV strains were higher than that of the primary culture of the feline peripheral blood mononuclear cells, indicating that this cell line should be useful for isolation, propagation, and neutralization test of FIV.
