Angioarchitecture and morphology of temporomandibular joint of Monodelphis domestica.

The opossum Monodelphis domestica presents movement of the temporomandibular joint (TMJ) reflecting adaptation to eating habits similar to movement in humans, but the structure of the TMJ is not yet known. Thus, nine young M. domestica, of both sexes were weighed, anesthetized with xylazine (10 mg kg(-1) ), and ketamine (70 mg kg(-1) ) and processed for: 1. The analyses of the macroscopic angioarchitecture after latex injection, as well as the topography of the TMJ; 2. The analysis of microvascularization after injection of Mercox resin and corrosion of soft tissue with NaOH using scanning electron microscopy and; 3. The histological evaluation of the TMJ with an optical microscope. Macroscopic analysis of the latex injected vessels revealed the distribution of the arteries from the common carotid artery, receiving branches of the superficial temporal and maxillary arteries. The mandibular condyle has the long axis in the lateral-lateral direction, and is convex in the anterior-posterior direction. Its topography was determined in relation to the eye and external acoustic meatus. With scanning electron microscopy, microvascularization consists of arterioles of varying diameter (85-15 µm) of the meandering capillary network in the retrodiscal region, and a network of straight capillaries in the TMJ anterior region. Via light microscopy the TMJ has similar histological features to those of humans. These macroscopic, microscopic and ultrastructural data from TMJ of the M. domestica could be a suitable model for TMJ physiology and pathophysiology studies for then speculate on possible human studies. Microsc. Res. Tech. 79:806-813, 2016. © 2016 Wiley Periodicals, Inc.

Preservación de Placenta Humana: Técnica Anatómica / Preservation of Human Placenta: Anatomical Technique

The fixation, conservation and preservation techniques, with academic and didactic aims, look for the way to find substances that maintain, as far as it is possible, the natural state of the body or organs, permeating the tissues, allowing an easy assembly, providing a detailed information and offering a three-dimensional view of the structures.The objective of the research was the view of angioarchitecture of the human placenta. The samples used were taken from the Servicio de Ginecología y Obstetricia, Unidad de Partos, del Hospital Hernán Enríquez Aravena, de Temuco, IX Región, Chile. All placentas were of a completed gestation condition, of single and normal childbirths.The placentas were injected with hardening solutions, and subject to corrosion with hydrochloric acid 32,4 %. This allowed the creation of a pattern of arteries and veins. Our results confirm that this technique can be useful in teaching and investigation, in view of the low costs and excellent results in the macroscopic visualization of the vascular plot of the human placenta.

Las técnicas de fijación, conservación y preservación, con fines didácticos y académicos, buscan la manera de encontrar sustancias que mantengan, dentro de lo posible, el estado natural del cuerpo u órganos, penetrando en los tejidos y permitiendo un fácil montaje, proporcionando información detallada y una visión tridimensional de las estructuras. Se planteó como objetivo del trabajo la visualización de la angioarquitectura de placenta humana, de gestación a término, partos normales, únicos, perteneciente al Servicio de Ginecología y Obstetricia, Unidad de Partos, del Hospital Hernán Enríquez Aravena, de Temuco, IX Región, Chile. Estas fueron inyectadas a través de los vasos placentarios con resina acrílica autopolimerizable, realizando luego, una corrosión por 11 días con HCL al 32,4 %, siendo hidrolizado completamente el parénquima y el estroma de la placenta, obteniéndose el molde de los vasos. Nuestros resultados confirmaron que esta técnica es útil en docencia e investigación, dado los bajos costos y excelente visualización macroscópica de la trama vascular de la placenta humana.

Angioarquitetura venosa do órgão genital da fêmea bovina / Venous angioarchitecture of the bovine female genital organ

Investigou-se a angioarquitetura venosa da genitália de fêmeas bovinas não prenhes, para avaliar a presença de possíveis anastomoses dos vasos provenientes da região da vulva e da vagina com a veia útero-ovariana, usando-se cinco peças obtidas em abatedouros. No laboratório, um ramo da veia vaginal caudal, infundido com contraste radiográfico intravascular, foi submetido à radiografia; Observou-se que a veia vaginal forma intensa rede de anastomoses na superfície ventral do útero, entre os antímeros direito e esquerdo. As genitálias apresentaram anastomoses dos vasos vulvares e vaginais com vasos da cérvice, corpo e cornos uterinos, sugerindo que parte de um agente luteolítico administrado via intravulvosubmucosa pode ser transportado diretamente aos ovários por uma rota local, sem atingir a circulação sistêmica.(AU)

The venous angioarchitecture of the non-pregnant female genital organs from five cattle obtained from a slaughterhouse was studied for possible anastomoses of the vulva and vagina vasa and the uterus-ovary vein, with the aid of the X-ray technique. In the laboratory, a branch of the caudal vaginal vein was injected with radiographic contrast. Vaginal vein formed and anastomotic network in the ventral surface of the uterus between the right and left sides. The genitals present anastomoses of the vulvar and vaginal veins with vasa of cervix, body and uterine horns and suggest that a portion of luteolytic agents injected through intravulvosubmucosal route can be transported directly to the ovary through a local route before reaching the systemic circulation.(AU)

Some features of the angioarchitecture of the testis in guinea-pig / Características da angioarquitetura do testículo de cobaios

Os ramos da artéria testicular em cobaio são uma artéria capsular principal e alguns ramos capsulares com disposições oblíqualongitudinal e transversa-longitudinal, mostrando um padrão distributivo variável em ambas as superfícies dos testículos. Os ramos subcapsulares se originam da própria artéria testicular ou da artéria capsular, sendo que estes ramos subcapsulares emitem vários outros ramos com disposição estratigrafica variável. Assim, se notam vasos sanguíneos extraalbugínicos colocados entre o estrato mesotelial e o próprio estrato denso da albugínea. Os vasos intra-albugínicos são caracterizados como seguimentos arteriasis e venosos dispostos na intimidade da albugínea testicular. Os vasos subcapsulares e intratesticulares sequentes aos anteriores aparecem na intimidade do testículo como pequenas artérias e veias, arteríolas e vênulas e capilares das redes capilares. Os vasos intratesticulares mostram um padrão complexo de disposição, sendo vistos, especialmente os capilares, com orientação espacial paralela ou perpendicular em relação aos túbulos seminíferos os quais circundam.(AU)