Bovine leukemia virus transmission rates in persistent lymphocytotic infected dairy cows.

Bovine leukemia virus (BLV) establishes a lifelong persistent infection in dairy cattle. White blood cell count (WBC) is correlated with proviral load in the blood and milk of BLV-infected cattle, and testing WBC can be used to assess both BLV infectiousness levels and risk of BLV transmission from different types of infected animals. The objective of the study was to compare effective transmission rates (ß) and the basic reproduction ratio (R o) among two types of BLV-infected dairy cows in Chile: those affected with persistent lymphocytosis (PL) vs. aleukemic (AL).The estimated (ß) coefficient was higher in PL cattle [1.1; 95% Confidence interval (CI) (-1.6, 3.8)], compared to AL cattle (-3.1; 95% CI = -3.7, -2.5). In addition, the R o was higher in PL cattle (60.4; 95% CI = 3.5; 820.6), compared to AL cattle (1.5; 95% CI = 0.7, 3.1). The ratio between PL/AL expected rate of cases was 73.9. The estimated effective transmission rate and the Ro were higher in PL cattle compared to AL cattle. The WBC test is a convenient alternative that can be considered for risk identification and risk management of BLV infection in dairy herds; particularly in livestock regions where laboratory capacity is limited (e.g., use of PCR or gene sequencing techniques) and/or molecular tests are not cost-effective. Therefore, when prevalence of infection is high, the removal of PL cattle should be engaged to control BLV within-herds.

Adjunctive photobiomodulation to Basic Periodontal therapy using different low-power laser application techniques: a systematic review and meta-analysis.

To review current literature and synthesize clinical outcomes related to different low-level laser techniques as a complement to basic periodontal therapy (BPT). Electronic searches were conducted in PubMed, Cochrane, and Scopus, and clinical trials published from January 2013 to August 2023 using photobiomodulation as a complement to basic periodontal therapy, with a clear description of the laser technique, were included. The risk of bias was assessed using the Joanna Briggs Institute Critical Assessment Checklist. Estimates of interest were calculated using random effects meta-analyses. A total of 947 references were retrieved, and 22 studies were included for qualitative synthesis. Ten studies used intrasulcular laser techniques, with 89% using infrared wavelength, and 12 studies used transgingival techniques, with 61.5% using red wavelength. The frequency of photobiomodulation after BPT ranged from 1 to 9 sessions, with follow-up periods ranging from 5 days to 12 months. Risk of bias was considered low in 16 studies and moderate in six studies. Meta-analysis of 13 studies showed that BPT reduced probing depth at 4-, 12- and 24-weeks post-treatment, and improved clinical level attachment at 6-, 12- and 24-weeks post-treatment. Studies suggest that photobiomodulation may be a valuable complement in the treatment of periodontitis, especially using transgingival application technique.

Desarrollo de habilidades motrices básicas en niños con trastorno por déficit de atención e hiperactividad, en Educación Física

Se estudia el desarrollo de habilidades motrices básicas en niños con trastorno por déficit de atención e hiperactividad y se planteó como objetivo diseñar y validar una estrategia didáctica inclusiva en una unidad educativa del Ecuador, con el empleo de una metodología mixta y pre experimental. Se utilizó un muestreo no probabilístico, intencional y estratificado, con criterio de inclusión, la muestra la conformaron cinco niños con trastorno por déficit de atención e hiperactividad, en su grupo, clase, y su docente de Educación Física. La medición de las variables inclusión-atención del trastorno con enfoque educativo y el desarrollo de habilidades motrices básicas, se realizó mediante ocho indicadores, con el empleo de una guía de observación, el análisis documental, y apoyada en la técnica de tarjado simple para agrupar los datos observados. Como resultado se expone una estrategia didáctica inclusiva para el desarrollo de las habilidades motrices básicas, en niños con trastorno por déficit de atención e hiperactividad, durante las clases de Educación Física, diseñada a través del enfoque sistémico estructural y funcional que mostró su efectividad en la práctica educativa, al comparar los resultados del pre- y postest, por medio de la prueba no paramétrica de los signos.

Estuda-se o desenvolvimento de habilidades motoras básicas em crianças com transtorno de déficit de atenção e hiperatividade e o objetivo foi desenhar e validar uma estratégia de ensino inclusiva em uma unidade educacional no Equador, utilizando uma metodologia mista e pré-experimental. Foi utilizada uma amostragem não probabilística, intencional e estratificada, com critérios de inclusão, a amostra foi composta por cinco crianças com transtorno de déficit de atenção e hiperatividade, em seu grupo, turma, e seu professor de Educação Física. A mensuração das variáveis ​​inclusão-atenção do transtorno com abordagem educativa e desenvolvimento de habilidades motoras básicas foi realizada por meio de oito indicadores, com uso de guia de observação, análise documental e apoiado na técnica de pontuação simples para agrupar os dados observados. Como resultado, apresenta-se uma estratégia didática inclusiva para o desenvolvimento de habilidades motoras básicas, em crianças com transtorno de déficit de atenção e hiperatividade, durante as aulas de Educação Física, desenhada através da abordagem sistêmica estrutural e funcional que mostrou sua eficácia na prática educativa, ao comparar os resultados do pré e pós-teste, por meio do teste não paramétrico de sinais.

The development of basic motor skills in children with attention deficit and hyperactivity disorder is studied and the objective was to design and validate an inclusive teaching strategy in an educational unit in Ecuador, using a mixed and pre-experimental methodology. An intentional and stratified non-probabilistic sampling was used, with inclusion criteria, the sample was made up of five children with attention deficit and hyperactivity disorder, in their class group, and their Physical Education teacher. The measurement of the inclusion-attention variables of the disorder with an educational approach and the development of basic motor skills was carried out using eight indicators, with the use of an observation guide, documentary analysis, and supported by the simple scoring technique to group the observed data. As a result, an inclusive didactic strategy is presented for the development of basic motor skills in children with attention deficit and hyperactivity disorder during Physical Education classes, designed through the structural and functional systemic approach that showed its effectiveness in the educational practice, when comparing the results of the pre- and post-test, through the non-parametric test of signs.

Production of biologically active human basic fibroblast growth factor (hFGFb) using Nicotiana tabacum transplastomic plants.

MAIN CONCLUSION: We generated transplastomic tobacco lines that stably express a human Basic Fibroblast Growth Factor (hFGFb) in their chloroplasts stroma and purified a biologically active recombinant hFGFb. MAIN: The use of plants as biofactories presents as an attractive technology with the potential to efficiently produce high-value human recombinant proteins in a cost-effective manner. Plastid genome transformation stands out for its possibility to accumulate recombinant proteins at elevated levels. Of particular interest are recombinant growth factors, given their applications in animal cell culture and regenerative medicine. In this study, we produced recombinant human Fibroblast Growth Factor (rhFGFb), a crucial protein required for animal cell culture, in tobacco chloroplasts. We successfully generated two independent transplastomic lines that are homoplasmic and accumulate rhFGFb in their leaves. Furthermore, the produced rhFGFb demonstrated its biological activity by inducing proliferation in HEK293T cell lines. These results collectively underscore plastid genome transformation as a promising plant-based bioreactor for rhFGFb production.

Schistosoma mansoni infection in residents of a riverside community in Eastern Amazon.

Schistosoma mansoni worms cause a waterborne parasitic disease called schistosomiasis. It commonly affects individuals in lack of sanitation structure. In Brazil, Pará state has Belém as one of the worst sanitation-ranking places in 2023, where schistosomiasis transmission was already documented. This study reports the occurrence of schistosomiasis in residents of Ilha das Onças, an island next to Belém. Stool samples were obtained from participants over 2 years old, all residents from Furo do Rio Grande, one of the rivers on the island. The Kato-Katz technique was performed for parasite investigation in the stool samples. Each participant responded to a sociodemographic and clinical questionnaire. The residences were georeferenced for map designing. Three out of 263 participants were S. mansoni positive, all men, ages ranging from 19 to 41 years old, with low parasitic load. Malacological surveys were carried out, but no Biomphalaria snails were found. Risk factors for schistosomiasis establishment are present on the island, and the lack of sanitation makes it a potential risk area. Malacological surveys are highly encouraged as preventive measures, as well as health surveillance for riverside populations, generating data that will help health authorities in the management and planning of preventive control actions.

Efectos del programa corporeidad para la adquisición de competencias motrices básicas en escolares de educación primaria

El estudio tuvo por objetivo determinar el efecto de la aplicación del Programa Corporeidad para la adquisición de competencias motrices básicas en escolares de educación primaria, Huanta-Ayacucho, 2023. La investigación fue aplicada, abordó desde el enfoque cuantitativo y diseño cuasi experimental. Participaron 107 estudiantes del 6° grado de primaria en dos grupos; solo al grupo experimental se aplicó el Programa de Corporeidad mediante 15 sesiones en las dimensiones percepción postural y percepción y regulación de móviles. Se aplicó la Batería MOBAK 5-6 midiendo dos competencias motrices básicas: control de objetos y control del cuerpo. Los resultados establecen que ambos grupos (control y experimental) en el pretest no reflejan diferencias significativas en el logro de las competencias motrices básicas (0.170 ≥ 0.05). Asimismo, se evidenció que ambos grupos (control y experimental) en el postest si reflejan diferencias significativas (0.000 < 0.05), encontrándose que el rango promedio del grupo control es 27.51 y del grupo experimental notoriamente más alto con 73.98, dando un valor para la prueba U de Mann-Whitney de 184,500; concluyendo que la aplicación del Programa Corporeidad si tuvo efecto significativo en la adquisición de las competencias motrices básicas en los escolares de educación primaria.

The objective of the study was to determine the effect of the application of the Corporeidad Program for the acquisition of basic motor skills in primary school students, Huanta-Ayacucho, 2023. The research was applied, approached from the quantitative approach and quasi-design experimental. 107 students from the 6th grade of primary school participated in two groups; The Corporeity Program was applied only to the experimental group through 15 sessions in the postural perception and mobile perception and regulation dimensions. The MOBAK Battery 5-6 was applied, measuring two basic motor skills: control of objects and control of the body. The results establish that both groups (control and experimental) in the pretest do not reflect significant differences in the achievement of basic motor skills (0.170 ≥ 0.05). Likewise, it was evidenced that both groups (control and experimental) in the post-test do reflect significant differences (0.000 < 0.05), finding that the average range of the control group is 27.51 and of the experimental group notoriously higher with 73.98, giving a value for the Mann-Whitney U test of 184,500; concluding that the application of the Corporeity Program did have a significant effect on the acquisition of basic motor skills in primary school students.

O objetivo do estudo foi determinar o efeito da aplicação do Programa Corporeidad para a aquisição de habilidades motoras básicas em alunos do ensino fundamental, Huanta-Ayacucho, 2023. A pesquisa foi aplicada, abordada a partir da abordagem quantitativa e quase-desenho experimental. Participaram 107 alunos do 6º ano do ensino fundamental em duas turmas; O Programa de Corporeidade foi aplicado apenas ao grupo experimental através de 15 sessões nas dimensões percepção postural e percepção móvel e regulação. Foi aplicada a Bateria MOBAK 5-6, medindo duas habilidades motoras básicas: controle de objetos e controle do corpo. Os resultados estabelecem que ambos os grupos (controle e experimental) no pré-teste não refletem diferenças significativas na obtenção de habilidades motoras básicas (0,170 ≥ 0,05). Da mesma forma, evidenciouse que ambos os grupos (controle e experimental) no pós-teste refletem diferenças significativas (0,000 < 0,05), verificandose que o intervalo médio do grupo controle é de 27,51 e do grupo experimental notoriamente superior com 73,98, dando um valor para o teste Mann-Whitney U de 184.500; concluindo que a aplicação do Programa Corporeidade teve um efeito significativo na aquisição de habilidades motoras básicas em alunos do ensino fundamental.

The importance of basic and translational research in caring for children with malignant solid tumors in Latin America.

Objective: Basic and translational research in pediatric cancer are essential to improve patient care. To critically assess the developments achieved in these areas in Latin America, we systematically reviewed information published between 2013 and 2023. Methods: Studies of basic and translational research performed by investigators in Latin America evaluating pediatric malignant solid and central nervous system tumors were retrieved from PubMed. Original articles published in English between 2013 and 2023 were included. Collaborations among Latin American authors or among Latin American authors working with researchers from other continents were also included. Studies were excluded if they focused only on adults or on basic research in tumor biology not specifically related to the tumor types analyzed in this review. Results: A total of 550 articles were retrieved, but after removal of duplicates, 514 articles were included in the analysis, the majority of which were authored by researchers affiliated with institutions in Argentina, Brazil and Mexico. These countries also had the highest number of collaborations on original articles published with authors from Europe and North America. Argentina had the highest number of collaborations on original publications, with coauthors from Brazil and Uruguay. The median impact factor of the 244 journals in which articles were published was 3.5. The most commonly studied tumors were osteosarcomas, neuroblastomas and medulloblastomas; the most commonly studied areas were molecular analysis, tumor cell biology and biomarkers. Conclusions: In Latin America, research in pediatric oncology is on the agenda, despite a notable disparity in publication rates and frequency of collaboration between countries. There is a need to strengthen scientific collaboration within Latin America and with countries from other continents to promote research and to develop novel treatment strategies that reflect the local needs of children in Latin America who have solid tumors and brain cancer.

Transmission dynamics of Zika virus with multiple infection routes and a case study in Brazil.

The Zika virus (ZIKV) is a serious global public health crisis. A major control challenge is its multiple transmission modes. This paper aims to simulate the transmission patterns of ZIKV using a dynamic process-based epidemiological model written in ordinary differential equations, which incorporates the human-to-mosquito infection by bites and sewage, mosquito-to-human infection by bites, and human-to-human infection by sex. Mathematical analyses are carried out to calculate the basic reproduction number and backward bifurcation, and prove the existence and stability of the equilibria. The model is validated with infection data by applying it to the 2015-2016 ZIKV epidemic in Brazil. The results indicate that the reproduction number is estimated to be 2.13, in which the contributions by mosquito bite, sex and sewage account for 85.7%, 3.5% and 10.8%, respectively. This number and the morbidity rate are most sensitive to parameters related to mosquito ecology, rather than asymptomatic or human-to-human transmission. Multiple transmission routes and suitable temperature exacerbate ZIKV infection in Brazil, and the vast majority of human infection cases were prevented by the intervention implemented. These findings may provide new insights to improve the risk assessment of ZIKV infection.

Persistent Barriers of the Gluten-Free Basic Food Basket: Availability, Cost, and Nutritional Composition Assessment.

Gluten-related disorders are treated with a gluten-free diet. The "basic food basket" (BFB) consists of a list of basic foods consumed by low-income groups in society, including those lowest-cost versions within each food category. To evaluate the cost, availability, and nutritional quality of the BFB and gluten-free BFB (GF-BFB), foods were photographed, registering their cost, availability, and nutritional characteristics, in high quality and mid-range supermarkets, wholesalers, health shops, and corner shops, matching each regular BFB product with a gluten-free equivalent. Of the 1177 potential products, the selection of lowest-cost foods yielded 55 and 47 products (BFB and GF-BFB, respectively). Breads/cereals and drinks showed the highest differences (279% and 146%, respectively) while meats and sausages showed the lowest ones (18.6%). The GF-BFB cost represents 30.1% of the minimum wage, which covers the cost of 5.2 and 3.3 of the BFB and GF-BFB per month, respectively. Availability ranged between 22.7 and 42.4%. Lower availability was associated with poorer nutritional quality in the GF-BFB, which provides 5% less energy, 26% more fat, and 25% less protein than the BFB. Only 47% of gluten-free products declared their "gluten-free" condition. The results strongly suggest that the GF-BFB must be redesigned to be both gluten-free and nutritionally adequate.

Assessment of preparedness and proficiency in basic and advanced life support among nursing professionals: a cross-sectional study.

Background: Cardiac diseases are among the leading causes of death worldwide, including sudden cardiac arrest in particular. Nursing professionals are often the first to encounter these scenarios in various settings. Adequate preparation and competent knowledge among nurses significantly impact survival rates positively. Aim: To describe the state of knowledge about Basic and Advanced Life Support guidelines among Ecuadorian nursing professionals. Methodology: A nationwide, descriptive, cross-sectional study was conducted from February to April 2023 among Ecuadorian nursing professionals. Participants were invited through official social media groups such as WhatsApp and Facebook. The study utilized a self-administered online questionnaire to evaluate theoretical knowledge of Basic Life Support (BLS) and Advanced Life Support (ALS). Knowledge scores were assigned based on the number of correct answers on the tests. T-tests and one-way ANOVA were used to examine relationships between knowledge scores and demographic and academic training variables. Results: A total of 217 nursing professionals participated in the study. The majority of the participants were female (77.4%) and held a university degree (79.9%). Among them, only 44.7% claimed to have obtained a BLS training certificate at least once, and 19.4% had ALS certification. The overall BLS knowledge score (4.8/10 ± 1.8 points) was higher than the ALS score (4.3/10 ± 1.8 points). Participants who had obtained BLS certification and those who used evidence-based summaries as a source of extracurricular training achieved higher BLS and ALS knowledge scores. Conclusion: Ecuadorian nursing professionals in this study exhibited a significant deficiency in theoretical knowledge of BLS and ALS. Formal training and preparation positively impact life support knowledge. Support and inclusion of Ecuadorian nurses in training and academic preparation programs beginning at the undergraduate level are essential for promoting life support knowledge and improving outcomes.

Dataset about Mexico's forest diversity: Site locations of tree species, wood densities, and geographic database of forest-vegetation provinces.

A dataset about three topics is provided, as a follow-up to the article "Mexico's forest diversity: common tree species and proposed forest-vegetation provinces" by Ricker et al. [1]. Firstly, 6927 site locations are provided for 22,532 trees of 1452 species. Secondly, measurements of basic wood-densities are reported for 779 tree species, obtained from 5256 trunk-core samples from Mexico's national forest inventory, and ranging from 0.05 to 0.93 g/cm3. Third, the data and maps of the forest-vegetation provinces from [1] were updated with the new cartography of Mexico's vegetation and land use (base year 2018). The maps are available now in an adjusted presentation as a shapefile-set for ArcGIS, as well as map-package and image files.

Spatial analysis of Dengue through the reproduction numbers relating to socioeconomic features: Case studies on two Brazilian urban centers.

The study of the propagation of infectious diseases in urban centers finds a close connection with their population's social characteristics and behavior. This work performs a spatial analysis of dengue cases in urban centers based on the basic reproduction numbers, R0, and incidence by planning areas (PAs), as well as their correlations with the Human Development Index (HDI) and the number of trips. We analyzed dengue epidemics in 2002 at two Brazilian urban centers, Belo Horizonte (BH) and Rio de Janeiro (RJ), using PAs as spatial units. Our results reveal heterogeneous spatial scenarios for both cities, with very weak correlations between R0 and both the number of trips and the HDI; in BH, the values of R0 show a less spatial heterogeneous pattern than in RJ. For BH, there are moderate correlations between incidence and both the number of trips and the HDI; meanwhile, they weakly correlate for RJ. Finally, the absence of strong correlations between the considered measures indicates that the transmission process should be treated considering the city as a whole.

Pepsin Inhibitors Prevent Inflammation and Loss of Laryngeal Barrier Function in Mice with Gastroesophageal Reflux.

OBJECTIVE: This study aimed to evaluate the role of pepsin inhibitors in the inflammatory response and their effects on laryngeal mucosal integrity during gastroesophageal reflux (GERD) under in vivo conditions. METHODS: A surgical model of GERD was used, in which mice were treated with pepstatin (0.3 mg/kg) or darunavir (8.6 mg/kg) for 3 days. On the third day after the experimental protocol, the laryngeal samples were collected to assess the severity of inflammation (wet weight and myeloperoxidase activity) and mucosal integrity (transepithelial electrical resistance and paracellular epithelial permeability to fluorescein). RESULTS: The surgical GERD model was reproduced. It showed features of inflammation and loss of barrier function in the laryngeal mucosa. Pepstatin and darunavir administration suppressed laryngeal inflammation and preserved laryngeal mucosal integrity. CONCLUSION: Pepsin inhibition by the administration of pepstatin and darunavir improved inflammation and protected the laryngeal mucosa in a mouse experimental model of GERD. LEVEL OF EVIDENCE: NA Laryngoscope, 134:3080-3085, 2024.

Changes in Food and Beverage Purchases Associated With the Coronavirus Disease Pandemic in Mexico.

BACKGROUND: Public policies to contain the spread of coronavirus disease in Mexico could have had an effect on food purchase patterns. OBJECTIVE: The objective of the study was to assess changes in the quantity of food and beverages purchased and proportion spent on food consumed away from home during the coronavirus disease pandemic in Mexican households. DESIGN: This study is a secondary analysis of cross-sectional data from the National Household Income and Expenditure Survey 2014-2020. PARTICIPANTS/SETTING: The study included 234,631 households with information on food and beverages purchases from 2014 to 2020. MAIN OUTCOME MEASURES: Food and beverage purchases were classified into six categories: basic, nonbasic energy-dense foods, prepared food for consumption at home, water, milk, and sugar-sweetened beverages. In the National Household Income and Expenditure Survey 2014-2020, expenditures on food and beverages consumed away from home are not classified into any specific items but represents more than 10% of food and beverage expenditures; therefore, the analyses included the proportion of food and beverage expenditures spent on food consumed away from home. STATISTICAL ANALYSES PERFORMED: A two-part model was used to evaluate changes in the quantity of food purchased and the proportion spent away from home during the coronavirus disease pandemic in 2020 compared with 2018, adjusting for socio-demographic variables, gross domestic product and previous survey rounds. Results are presented at the national level, by income quintile and by place of residence. RESULTS: Purchases of basic food increased by 17 g/capita/day at the national level and 22.4 g/capita/day in urban areas (P < 0.001). Purchases of nonbasic energy-dense foods decreased both at the national level (-4.2 g/capita/day; P < 0.001) and by place of residence (-4.8 g/capita/day; P < 0.001 in urban areas and -2.5 g/capita/day; P = 0.001 in rural settings). Purchases of prepared food increased 16 g/capita/day (P < 0.001). In rural areas, purchases of sugar-sweetened beverages increased 7.2 mL/per capita/day (P < 0.001). For the lowest income quintile purchases of processed meat increased 2.4 g/capita/day (P < 0.001). The proportion spent on food consumed away from home decreased by -44.9% (P < 0.001). CONCLUSIONS: Results of this study show that during the coronavirus disease lockdown there was an increase in basic and prepared food purchases, whereas purchases of nonbasic energy-dense foods and the proportion spent on food consumed away from home decreased. However, findings showed an increase in sugar-sweetened beverages purchases among rural dwellers and an increase in purchases of processed meat among the lowest income quintile. Various factors such as income reductions, unemployment, mobility restrictions, or increases in prices may have led the observed changes. Future research should be conducted to analyze these potential pathways.

Removal of dyes from water using Citrullus lanatus seed powder in continuous and discontinuous systems.

The objective of this study is to develop a low-cost biosorbent using residual seeds of the Citrullus lanatus fruit for the removal of cationic dyes. Physicochemical parameters such as pH, adsorbent mass, contact time, and temperature were evaluated for their effects on dye removal. The biosorbent is composed of lignin and cellulose, exhibiting a highly heterogeneous surface with randomly distributed cavities and bulges. The adsorption of both dyes was most effective at natural pH with a dosage of 0.8 g L-1. Equilibrium was reached within 120 min, regardless of concentration, indicating rapid kinetics. The Elovich model and pseudo-second-order kinetics were observed for crystal violet and basic fuchsin dye, respectively. The Langmuir model fitted well with the equilibrium data of both dyes. However, the increased temperature had a negative impact on dye adsorption. The biosorbent also demonstrated satisfactory performance (R = 43%) against a synthetic mixture of dyes and inorganic salts, with a small mass transfer zone. The adsorption capacities for crystal violet and basic fuchsin dye were 48.13 mg g-1 and 44.26 mg g-1, respectively. Thermodynamic studies confirmed an exothermic nature of adsorption. Overall, this low-cost biosorbent showed potential for the removal of dyes from aqueous solutions.

In this work, a novel biosorbent was developed using residual Citrullus lanatus fruit seeds that can efficiently remove cationic dyes from aqueous solutions. The biosorbent's composition includes lignin and cellulose, and its surface structure is highly heterogeneous, consisting of randomly distributed cavities and bulges. The biosorbent demonstrated a rapid and efficient adsorption capacity for both crystal violet and basic fuchsin, regardless of dye concentration. Moreover, the biosorbent was successfully employed in the treatment of a synthetic mixture containing several dyes and inorganic salts. Finally, the application of the biosorbent in continuous adsorption showed a low zone of mass transfer and high breakthrough time, indicating it to be an excellent material for fixed-bed operation. Overall, this study provides a low-cost and efficient alternative for the removal of dyes from aqueous solutions, with promising practical applications.

Effects of the preoperative use of artificial tears combined with recombinant bovine basic fibroblast growth factor on cataract patients complicated with dry eyes / Efeitos do uso pré-operatório de lágrimas artificiais combinadas com fator de crescimento de fibroblastos básicos bovinos recombinantes em pacientes com catarata complicada com olhos secos

ABSTRACT Purpose: To assess the effects of the preoperative application of artificial tears combined with recombinant bovine basic fibroblast growth factor on the ocular surface function and inflammatory factor levels after operation in cataract patients complicated with dry eyes. Methods: A total of 118 cataract patients (118 eyes) complicated with dry eyes treated from February 2019 to February2020 were assigned to control and observation groups (n=59 eyes/group) using a random number table. One week before the operation, the control group was administered 0.1% sodium hyaluronate eye drops (artificial tears), based on which the observation group received Beifushu eye drops (recombinant bovine basic fibroblast growth factor), both 6 times daily for 1 week. A comparison was made between the scores of clinical symptoms and the indices of ocular surface function, inflammatory factors in tears, and oxidative stress indices before and after the operation. The ocular surface function was evaluated by an ocular surface disease index questionnaire, tear film breakup-time assay, Schirmer's I test, and corneal fluorescein stain test. The inflammatory factors in tears were measured. Results: No significant differences were noted in the general data and clinical symptom score, ocular surface disease index, tear film breakup-time, Schirmer's I test score, fluorescein stain score, interleukin-6, tumor necrosis factor-alpha, malondialdehyde, superoxide dismutase, lipid peroxide, and total antioxidant capacity before treatment between the 2 groups (p>0.05). After treatment, the clinical symptom score, ocular surface disease index, fluorescein stain score, tumor necrosis factor-alpha, interleukin-6, malondial-dehyde and lipid peroxide declined significantly, and tear film breakup-time, Schirmer's I test score, superoxide dismutase, and total antioxidant capacity increased in both the groups. The improvements in the clinical symptom score as well as in the indices of ocular surface function, inflammatory factors, and oxidative stress were more prominent in the observation group than in the control group (p<0.05). Conclusions: Artificial tears combined with recombinant bovine basic fibroblast growth factor before operation. significantly improved the ocular surface function, reduced inflammatory factors in tears, and alleviated dry eye symptoms after operation in cataract patients.

RESUMO Objetivo: Avaliar os efeitos da aplicação pré-operatória de lágrimas artificiais combinadas com o fator de crescimento de fibroblastos básicos bovinos recombinantes na função da superfície ocular e níveis de fator inflamatório após cirurgia em pacientes com catarata complicada com olhos secos. Métodos: Um total de 118 pacientes com catarata complicada com olhos secos (118 olhos), tratados entre fevereiro de 2019 e fevereiro de 2020, foram divididos em grupos de controle e de observação (n=59, 59 olhos) usando uma tabela de números aleatórios. Uma semana antes da cirurgia, o grupo controle recebeu colírio de hialuronato de sódio a 0,1% (lágrimas artificiais), enquanto o grupo de observação recebeu colírio Beifushu (fator de crescimento de fibroblastos básicos bovinos recombinantes), ambos, seis vezes ao dia, por uma semana. Antes do tratamento e um mês após a cirurgia, os escores de sintomas clínicos, índices de função da superfície ocular, níveis de fatores inflamatórios nas lágrimas e índices de estresse oxidativo foram comparados. A função da superfície ocular foi avaliada pelo questionário do índice de doença da superfície ocular, ensaio de tempo de ruptura do filme lacrimal, teste I de Schirmer e teste de coloração por fluoresceína da córnea. Os níveis de fatores inflamatórios nas lágrimas foram medidos. Resultados: Não houve diferenças significativas nos dados gerais e no escore de sintomas clínicos, índice de doença da superfície ocular, tempo de ruptura do filme lacrimal, escore do teste I de Schirmer, pontuação do teste de coloração por fluoresceína da córnea, interleucina-6, fator de necrose tumoral alfa, malondialdeído, superóxido dismutase, peróxido lipídico e capacidade antioxidante total antes do tratamento entre os dois grupos (p>0,05). Após o tratamento, o escore de sintomas clínicos, índice de doença da superfície ocular, escore do teste de coloração por fluoresceína da córnea, fator de necrose tumoral alfa, interleucina-6, malondialdeído e peróxido lipídico diminuíram significativamente, e o tempo de ruptura do filme lacrimal, escore do teste I de Schirmer, superóxido dismutase e a capacidade antioxidante total aumentou em ambos os grupos. As melhorias no escore de sintomas clínicos, bem como os índices de função da superfície ocular, fatores inflamatórios e estresse oxidativo foram mais proeminentes no grupo de observação do que no grupo controle (p<0,05). Conclusões: Lágrimas artificiais combinadas com fator de crescimento de fibroblastos básicos recombinantes antes da cirurgia melhoram notavelmente a função da superfície ocular, diminuem os níveis de fatores inflamatórios nas lágrimas e aliviam os sintomas de olho seco após a cirurgia em pacientes com catarata complicada com olhos secos.

Realização periódica do Papanicolau: uma contribuição de empoderamento através de oficina / Periodic realization of Papanicolaou: a contribution to the empowerment of women / Realización periódica de Papanicolaou: una contribución al empoderamiento de la mujer

Objetivo: desenvolver uma oficina como forma de contribuição ao empoderamento de mulheres para a realização periódica do Papanicolau. Método: qualitativo, exploratório e descritivo, tipo pesquisa participante, em uma unidade de saúde, com mulheres entre 25 a 59 anos, através de entrevista semiestruturada coletiva, durante uma oficina ocorrida em fevereiro de 2023, analisada por meio da Análise Temática de Conteúdo. Resultados: a oficina mostrou-se como um dispositivo que potencializa o empoderamento feminino, interferindo no processo saúde-doença. Desvelou-se ainda como ferramenta de dialogicidade e escuta ativa, na compreensão dos determinantes que se configuram como empecilhos na realização periódica do Papanicolau. Considerações finais: como forma de romper barreiras, ao tratar-se da periodicidade do Papanicolau, a oficina é uma ferramenta eficiente e incentivadora de promoção à participação ativa, à autonomia, à autoestima e ao empoderamento social por meio do processo educativo

Objective: develop a workshop as a way of contributing to the empowerment of women to carry out regular Pap smears. Method: qualitative, exploratory and descriptive, participant research type, in a health unit, with women between 25 and 59 years old, through collective semi-structured interviews, during a workshop, analyzed through Thematic Content Analysis. Results: the workshop proved to be a device that enhances female empowerment, interfering in the health-disease process. It also revealed itself as a tool for dialogue and active listening, in understanding the determinants that constitute obstacles in the periodic performance of the Pap smear. Final considerations: as a way of breaking down barriers, when it comes to the frequency of the Pap smear, the workshop is an efficient and encouraging tool to promote active participation, autonomy, self- esteem and social empowerment through the educational process

Objetivos: desarrollar un taller como forma de contribuir al empoderamiento de las mujeres para la realización periódica de Papanicolaou. Método: investigación cualitativa, exploratoria y descriptiva, tipo participante, en una unidad de salud, con mujeres entre 25 y 59 años, a través de entrevistas colectivas semiestructuradas, durante un taller, analizadas mediante Análisis de Contenido Temático. Resultados: el taller resultó ser un dispositivo que potencia el empoderamiento femenino, interfiriendo en el proceso salud-enfermedad. También se reveló como una herramienta de diálogo y escucha activa, en la comprensión de los determinantes que constituyen obstáculos en la realización periódica del Papanicolaou. Consideraciones finales: como una forma de romper barreras en cuanto a la frecuencia de la prueba de Papanicolaou, el taller es una herramienta eficiente y alentadora para promover la participación activa, la autonomía, la autoestima y el empoderamiento social a través del proceso educativo

A relevância em abordar suporte básico de vida para estudantes em escolas públicas e privadas / The relevance of addressing basic life support for students in public and private schools / La relevancia de abordar el soportevital básico para estudiantes en escuelas públicas y privadas

Objetivo:Investigar a relevância da abordagem de suporte básico de vida aos estudantes nas escolas públicas e privadas. Método:Trata-se de um trabalho constituído através de um estudo descritivo, exploratório, por intermédio de uma revisão sistemática, utilizandoestratégias qualitativas de pesquisa. Ao todo, nove artigos foram escolhidos, cujos critérios foram inclusão, exclusão e éticos. Posteriormente houve a leitura dos textos completos e descarte de conteúdos que fugiam da temática. Utilizou-se como base de dados a Biblioteca Virtual em Saúde e a PUBMED (Serviço da U. S. National Library of Medicine). Resultados:Verificou-se que os estudantes que tiveram contato com a temática Suporte Básico de Vida, por meio de simulações e questionários, se mostraram mais preparados para salvar vidas, prevenir acidentes e reduzir danos. Visto que, as emoções e o desenvolvimento de habilidades foram diretamente ligadas, pois, as emoções positivas contribuíram para maior adesão das informações e desenvolvimento de habilidades. Considerações Finais: Assim sendo, os estudantes entendem a relevância de abordar esse assunto e com treinamento contínuo nas escolas a longo prazo irá manter a expertise dos alunos, garantindo chances altas para salvar uma vida.

Objective:To investigate the relevance of the basic life support approach to students in public and private schools. Method:This is a work constituted through a descriptive, exploratory study, through an integrative review, using qualitative research strategies. In all, nine articles were chosen, whose criteria were inclusion, exclusion and ethical. Subsequently, the complete texts were read and contents that deviated from the theme were discarded. The Virtual Health Library and PUBMED (Service of the U.S. National Library of Medicine) were used as a database. Results:It was found that students who had contact with the theme Basic Life Support, through simulations and questionnaires, were more prepared to save lives, prevent accidents and reduce harm. Since emotions and skill development were directly linked, as positive emotions contributed to greater adherence to information and skill development. Final considerations:Therefore, students understand the relevance of addressing this subject and with continuous training in schools in the long term, students' expertise will be maintained, guaranteeing high chances of saving a life.

Objetivo:Investigar la pertinencia del abordaje de soporte vital básico para estudiantes de escuelas públicas y privadas. Método: Este es un trabajo constituido a través de un estudio descriptivo, exploratorio, a través de una revisión integradora, utilizando estrategias de investigación cualitativa. En total, se eligieron nueve artículos, cuyos criterios fueron de inclusión, exclusión y éticos. Posteriormente, se leyeron los textos completos y se descartaron los contenidos que se desviaban del tema. Se utilizó como base de datos la Virtual Health Library y PUBMED (Service of the U.S. National Library of Medicine). Resultados: Se constató que los estudiantes que tuvieron contacto con el tema Soporte Básico de Vida, a través de simulacros y cuestionarios, estaban máspreparados para salvar vidas, prevenir accidentes y reducir daños. Dado que las emociones y el desarrollo de habilidades estaban directamente relacionados, las emociones positivas contribuyeron a una mayor adherencia a la información y al desarrollo de habilidades. Consideraciones Finales: Por lo tanto, los estudiantes entienden la relevancia de abordar este tema y con la formación continua en las escuelas a largo plazo, se mantendrá la experiencia de los estudiantes, garantizando altas posibilidades de salvar una vida

Trilha Digital Gamificada: Relato de experiência de um trabalho colaborativo entre Universidade e Escola / Gamified Digital Trail: Experience report of collaborative work between University and School / Sendero Digital Gamificado: Informe de experiencia de trabajo colaborativo entre Universidad y Escuela

tendo como foco a criação de um recurso pedagógico digital inovador destinado a aprimorar o ensino de fisiologia humana na Escola Básica. Método:Trata-se de um relato de experiência, de caráter descritivo, acerca das vivências de professores da Universidade Estadual de Feira de Santana e de uma Escola Pública do Estado da Bahia, entre outubro de 2022 a outubro de 2023. As atividades foram organizadas em duas etapas interconectadas: 1) Realização de um Curso de formação de Educação à Distância (EaD) durante a Semana Nacional de Ciência e Tecnologia (SNCT). 2) Elaboração de um material didático inovador envolvendo as tecnologias digitais educacionais, como produto final do Curso EaD realizado na SNCT. Resultados:Elaboração do jogo de tabuleiro, intitulado jogo de trilha gamificada denominada "Trilha Corpo Humano: Conhecer para Cuidar". Conclusão:A proposta desenvolvida no presente trabalho vislumbrou a inovação da prática pedagógica, por meio do desenvolvimento de novas tecnologias educacionais, contribuindo para a melhoria do ensino na Escola.

Objective: To report the experience of integrating University and Primary School Teachers, focusing on the creation of an innovative digital pedagogical resource aimed at improving the teaching of human physiology in Primary Schools. Method:This is an experience report, of a descriptive nature, about the experiences of teachers at the State University of Feira de Santana and a Public School in the State of Bahia, between October 2022 and October 2023. The activities were organized in two interconnected stages: 1) Carrying out a Distance Education (EaD) training course during National Science and Technology Week (SNCT). 2) Creation of innovative teaching material involving digital educational technologies, as the final product of the Distance Learning Courseheld at SNCT. Results:Preparation of the board game, entitled gamified trail game called "Human Body Trail: Knowing to Care". Conclusion: The proposal developed in this work envisioned the innovation of pedagogical practice, through the development of new educational technologies, contributing to the improvement of teaching at School.

Objetivo: Reportar la experiencia de integración de Profesores Universitarios y de Educación Primaria, centrándose en la creación de un recurso pedagógico digital innovador orientado a mejorar la enseñanza de la fisiología humana en la Escuela Primaria. Método: Se trata de un relato de experiencia, de carácter descriptivo, sobre las vivencias de docentes de la Universidad Estatal de Feira de Santana y de una Escuela Pública del Estado de Bahía, entre octubre de 2022 y octubre de 2023. Las actividades se organizaron en dos espacios interconectados. etapas: 1) Realización de un curso de capacitación en Educación a Distancia (EaD) durante la Semana Nacional de Ciencia y Tecnología (SNCT). 2) Desarrollo de material didáctico innovador que involucre tecnologías educativas digitales, como producto final del Curso a Distancia realizado en el SNCT. Resultados:Elaboración del juego de mesa, titulado Juego de Sendero gamificado denominado "Human Body Trail: Saber Cuidar". Conclusión:La propuesta desarrollada en este trabajo vislumbró la innovación de la práctica pedagógica, a través del desarrollo de nuevas tecnologías educativas, contribuyendo a la mejora de la enseñanza en la Escuela.

Necessidades Formativas do Professor Frente à Demanda de Alunos da Educação Especial em Classes Comuns / Teacher's Training Needs Towards Special Education Students' Demand in Ordinary Classrooms

RESUMO: Este estudo teve como escopo analisar os avanços da inclusão do público da Educação Especial nas salas de aula comuns da Educação Básica, considerando as necessidades formativas dos docentes nessa área específica. Consiste em um estudo quantiqualitativo, tendo como fontes empíricas microdados da Prova Brasil, edição 2021, mais especificamente os questionários destinados aos docentes do 5º ano do Ensino Fundamental, os quais estão disponíveis no portal do Instituto Nacional de Estudos e Pesquisas Educacionais Anísio Teixeira (Inep). A extração dessas informações e análise estatística foram realizadas com o auxílio do Statistical Package for Social Science (SPSS). O resultado da investigação revela que o percentual de matrículas dos alunos com deficiência, transtorno do espectro autista (TEA) e altas habilidades em classes comuns, no Brasil, entre 2013 e 2019, cresceu 7,4%. Aponta, também, que 86,5% dos professores da amostra investigada declararam possuir necessidades formativas de nível moderado a alto. Conclui-se que o público da Educação Especial ainda necessita travar muitos embates em prol de uma escola mais justa e inclusiva para todos, o que implica batalhar para que os princípios de igualdade e equidade se materializem tanto no campo real quanto no campo ideal.

ABSTRACT: This study focused on analyzing the inclusive advances for the population of Special Education in ordinary classrooms in Basic Education, considering teachers' training need in this specific area. It consists of a quantitative and qualitative approach study, using as empirical sources microdata from Prova Brasil, 2021 edition, more specifically the questionnaires applied to teachers of the 5th grade of Elementary School, which are available on the webpage of the National Institute of Educational Studies and Research Anísio Teixeira (INEP). The extraction of this information and statistical analysis were carried out with the aid of the Statistical Package for Social Science (SPSS). The result of the investigation reveals that the percentage of enrollments for students with disabilities, autism spectrum disorder (ASD) and giftedness in ordinary classrooms, in Brazil, between 2013 and 2019, rose 7.4%. It also points out that 86.5% of the teachers in the investigated sample declared that they had moderate to high training needs. It was concluded that the population of Special Education still needs to face some issues in favor of a fairer and more inclusive school to everyone, which implies fighting so that the principles of equality and equity materialize both in the real field and in the ideal field.