[Acupuncture combined with Qingfei Qutan decoction for stroke-associated pneumonia with phlegm-heat obstructing lung and its effect on cellular immune function].

OBJECTIVE: To observe the clinical effect of acupuncture combined with Qingfei Qutan decoction for stroke-associated pneumonia (SAP) with phlegm-heat obstructing lung, and explore its possible mechanism. METHODS: Ninety-nine patients of SAP with phlegm-heat obstructing lung were randomly divided into a combination group (33 cases, 1 case dropped off), a Chinese medication group (33 cases, 1 case dropped off) and an acupuncture group (33 cases, 1 case dropped off). On the basis of routine basic treatment, the patients in the acupuncture group were treated with acupuncture at Tiantu (CV 22), Feishu (BL 13), Taiyuan (LU 9), Sanyinjiao (SP 6), etc., once a day, with an interval of 1 day after continuous 6-day treatment; the patients in the Chinese medication group were treated with Qingfei Qutan decoction, 1 dose per day; the patients in the combination group were treated with acupuncture combined with Qingfei Qutan decoction. Two weeks were taken as a course of treatment, and two courses of treatment were given. Before and after treatment, the clinical pulmonary infection score (CPIS), inflammatory indexes (neutrophil-to-lymphocyte ratio [NLR], procalcitonin [PCT], C-reactive protein [CRP]), cellular immune function (CD+3, CD+4, CD+8 and CD+4/CD+8) were compared in the 3 groups. The clearance of pathogenic bacteria after treatment was observed in the 3 groups. The clinical efficacy of each group was evaluated. RESULTS: After treatment, the CPIS scores, NLR, PCT, CRP and CD+8 in the each group were lower than those before treatment (P<0.05), while the levels of CD+3, CD+4, CD+4/CD+8 were higher than those before treatment (P<0.05). The above indexes in the combination group were better than those in the acupuncture group and the Chinese medication group (P<0.05), and the above indexes in the Chinese medication group were better than those in the acupuncture group (P<0.05). There was no significant difference in the clearance rate of pathogenic bacteria among three groups (P>0.05). The cured and markedly effective rate was 65.6% (21/32) in the combination group, which was higher than 43.8% (14/32) in the Chinese medication group and 18.8% (6/32) in the acupuncture group (P<0.05). The cured and markedly effective rate in the Chinese medication group was higher than that in the acupuncture group (P<0.05). CONCLUSION: Acupuncture combined with Qingfei Qutan decoction could effectively improve the clinical symptoms of SAP patients with phlegm-heat obstructing lung, and the mechanism may be related to enhancing the cellular immune function and reducing the level of inflammatory reaction.

Expression of serum LAIR2 in patients with non-small cell lung cancer and its relationship with cellular immune function / 中华内分泌外科杂志

Objective:To investigate the relationship between serum leukocyte-associated immunoglobulin-like receptor 2 (LAIR2) expression and cellular immune function, prognosis in patients with non-small cell lung cancer.Methods:From April 2016 to April 2017, 90 patients with non-small cell lung cancer who were treated in Shangqiu first people’s Hospital were taken as the lung cancer group, and they were grouped into the survival group and the death group according to whether the patients died within 5 years. Another 84 patients with benign pulmonary mass were selected as the control group. The levels of CD4+[ (28.26±5.14) % vs (47.02±6.73) %], CD8+ [ (23.76±5.84) % vs (30.12±6.03) %] and CD4+/CD8+ [ (1.17±0.30) % vs (1.56±0.50) %] in peripheral blood immune cells were detected by flow cytometry; the serum LAIR2 level was detected by enzyme-linked immunosorbent assay (ELISA) , and the average serum LAIR2 level of patients with non-small cell lung cancer was applied as the boundary, and they were grouped into a low LAIR2 expression group and a LAIR2 high expression group; Pearson correlation was applied to analyze the correlation between serum LAIR2 level and immune cell level in patients with non-small cell lung cancer; Kaplan-Meier curve was applied to analyze the relationship between serum LAIR2 level and 5-year survival rate of patients with non-small cell lung cancer; and multivariate COX regression analysis was applied to analyze the factors affecting 5-year mortality in patients with non-small cell lung cancer.Results:The level of serum LAIR2 [ (69.55±13.12) ng/ml vs. (20.64±7.13) ng/ml] in the lung cancer group was significantly higher than that in the control group, and the levels of CD4 +, CD8 + and CD4+/CD8+ were significantly lower than those in the control group ( P<0.05) . The serum LAIR2 level in patients with TNM stage III+IV (77.32±13.09) ng/ml, poorly differentiated tissue (78.14±13.26) ng/ml, and lymph node metastasis (79.02±13.81) ng/ml was significantly higher than that in patients with TNM stage I+II (64.37±12.89) ng/ml, medium/well differentiated tissue (64.32±12.73) ng/ml, and no lymph node metastasis (62.92±12.85) ng/ml ( P<0.05) . The levels of CD4 +, CD8 + and CD4 +/CD8 + in the LAIR2 high expression group were significantly lower than those in the LAIR2 low expression group ( P<0.05) . Serum LAIR2 level in patients with non-small cell lung cancer was negatively correlated with CD4+, CD8+ and CD4+/CD8+ levels ( r=-0.510, -0.496, -0.494, P<0.05) . The 5-year survival rate of patients with high LAIR2 expression was lower than that of patients with low LAIR2 expression ( r=6.375, P<0.05) . LAIR2 was an independent risk factor for 5-year death in patients with non-small cell lung cancer ( P<0.05) . Conclusion:LAIR2 is highly expressed in serum of patients with non-small cell lung cancer, and its expression level is closely related to cellular immune function and prognosis.

Effects of HBeAg Status on cellular immune function of patients with Hepatitis B virus/Treponema Pallidum Co-infectio.

OBJECTIVES: This study was aimed at exploring the effects of hepatitis B envelope antigen (HBeAg) status on the cellular immune function of patients with hepatitis B virus/treponema pallidum (HBV/TP) co-infection. METHODS: The clinical data of 79 patients with HBV/TP co-infection admitted to our hospital from January 2019 to January 2020 were retrospectively analyzed. These patients were divided into two groups according to the different HBeAg statuses before the treatment: 41 HBeAg+ patients were included in the HBeAg+ group, while 38 HBeAg- patients were included in the HBeAg- group. The levels of HBV-DNA, T lymphocyte subsets represented by NK cells and cytokines associated with T cells in the peripheral blood (PB) of the patients were compared between both groups. RESULTS: The HBV-DNA levels in the HBeAg+ group were significantly higher than those in the HBeAg- group (P < 0.05). The levels of CD3+, CD4+, CD4+/CD8+ and natural killer (NK) cells in the HBeAg+ group were higher than those in the HBeAg- group (P < 0.05), while the levels of CD8+ cells were lower than those in the HBeAg- group (P < 0.05). Moreover, the levels of interferon-γ (IFN-γ), tumor necrosis factor (TNF-α), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-17 (IL-17), transforming growth factor-ß (TGF-ß) in the HBeAg+ group were all significantly higher than those in the HBeAg- group (P < 0.05), but there was no significant difference in the levels of interleukin-4 (IL-4) and interleukin-10 (IL-10) between the HBeAg+ group and the HBeAg- group (P > 0.05). CONCLUSION: HBeAg+ can increase the HBV-DNA levels in the PB of patients with HBV/TP co-infection, in turn triggering the body to initiate cellular immunity, increasing the levels of T lymphocyte subsets, and promote the secretion of cytokines.

A randomised trial: effects of different anesthesia methods on early perioperative pain sensitivity and cellular immune function in patients undergoing radical mastectomy.

BACKGROUND: This study sought to investigate the effects of transversus thoracic muscle plane-pectoral nerves (TTP-PECS) block combined with propofol anesthesia on early perioperative pain sensitivity and cellular immune function in patients undergoing radical mastectomy. METHODS: A total of 115 patients who underwent radical mastectomy for breast cancer at our hospital from January 2019 to January 2021 were selected as the study subjects. The patients were allocated to the control group (n=57) or observation group (n=58) using a random number method. The control group was given simple general anesthesia, and the observation group was given TTP-PECS block combined with propofol anesthesia. The recovery time, pain [visual analogue scoring (VAS)] scores, and incidences of adverse reactions were compared between the 2 groups. Hemodynamic indicators [i.e., heart rate (HR), mean arterial pressure (MAP)], stress indicators [i.e., blood glucose (GLU), epinephrine (E), cortisol (Cor)], and the cellular immune function ofthe2 groups before anesthesia (T0), at the end of operation (T1), 1day after operation (T2) and 3days after operation (T3) were recorded. RESULTS: The spontaneous respiration recovery time, time to full wakefulness and the extubation time of the observation group were shorter than those of the control group (P<0.05). The observation group had lower VAS scores than the control group at 2, 8, 12, and 24 h after operation (P<0.05). The levels of MAP, HR, GLU, E and Cor in the observation group at T1, T2, and T3 were lower than those in the control group (P<0.05). Compared to the control group, the observation group had increased cluster of differentiation (CD)3+, CD4+, and CD4+/CD8+ cells (P<0.05), but there were no significant differences in CD8+ and natural killer (NK) cells between the 2 groups (P>0.05). The incidence of adverse reactions in the observation group was lower than that in the control group (8.62% vs. 24.56%) (P<0.05). CONCLUSIONS: TTP-PECS block combined with propofol anesthesia can relieve pain, shorten the recovery time, stabilize the hemodynamic level, and alleviate the stress responses of patients undergoing radical mastectomy with a slight suppression of cellular immune function and high safety. TRIAL REGISTRATION: Chinese Clinical Trial Registration Center ChiCTR2100048438.

Analysis of the SARS-CoV-2 specific antibodies and immune function of COVID-19 patients one year after discharge / 中国胸心血管外科临床杂志

@#Objective    To summarize the results of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgG antibody, total antibody and cellular immune function of COVID-19 convalescent patients one year after discharge, and to analyze the correlation between the SARS-CoV-2 antibody and the indexes of immune function. Methods    A total of 41 confirmed COVID-19 patients discharged from Chengdu Public Health Clinical Medical Center from January to April 2020 and followed up one year after discharge were included in the study as the research group, including 18 males and 23 females with an average age of 47.83±12.95 years. The results of SARS-CoV-2 IgG, total antibody and immune function indexes one year after discharge were collected in order to discuss the correlation of SARS-CoV-2 and cellular immune function. A total of 40 healthy employees of the hospital vaccinated against COVID-19 were randomly selected as the vaccine group, including 10 males and 30 females with an average age of 43.90±6.86 years. The SARS-CoV-2 antibodies between the two groups were compared. Results    CD8+T cell count was higher and CD4+T/CD8+T was lower in male patients than those in female patients (all P<0.05). The IgG and total antibodies in patients with re-detectable positive RNA test were both higher than those in patients without re-detectable positive RNA test, but the differences were not statistically significant (P=0.158, 0.060). The positive rate of SARS-CoV-2 IgG in the research group was 80.5% (33/41). SARS-CoV-2 IgG was positively correlated with total antibody (P<0.001). There was a positive correlation between CD4+T cell count and SARS-CoV-2 IgG (r=0.455, P=0.003). The positive rate of SARS-CoV-2 IgG, SARS-CoV-2 IgG amount and total antibody amount in the research group were significantly higher than those in the vaccine group (all P<0.001). Conclusion    SARS-CoV-2 IgG of most COVID-19 patients one year after discharge is positive, and their SARS-CoV-2 total antibody is significantly higher than people vaccinated against COVID-19, which suggests that patients infected with SARS-CoV-2 can obtain lasting protection, but the protection may be gradually weaken over time. The degree of antibody attenuation in patients with re-detectable positive RNA test may be weaker. In the convalescence stage, the dynamics of SARS-CoV-2 IgG may be closely related to cellular immune function.

A practical guide for assessing respiratory burst and phagocytic cell activity in the fathead minnow, an emerging model for immunotoxicity.

Measures of respiratory burst and phagocytic cell activity are frequently utilized to assess cellular immune function in teleosts. Respiratory burst predominately occurs in neutrophils and causes the release of reactive oxygen species to kill pathogens. Phagocytosis is the process by which pathogens are engulfed and destroyed by various immune cells. Though a variety of approaches have been utilized to measure respiratory burst and phagocytic cell activity, assays that rely only on common laboratory equipment (e.g., plate reader) may offer advantages over those that rely on more specialized equipment (e.g., flow cytometer). The goal of the current study was to optimize and validate the use of a colorimetric plate-based respiratory burst and fluorometric plate-based phagocytic cell activity assays for use with kidney cells from the fathead minnow (Pimephales promelas), an emerging immunotoxicity model. In addition, a protocol for the dissection of kidney tissue followed by the extraction of kidney cells, as well as recommendations and resources for future experiments utilizing each of these assays, are provided.•All methods are optimized for use with the fathead minnow or similar teleost species.•Respiratory burst and phagocytic cell activity are measured using a standard plate reader.

Therapeutic effect of Saccharomyces boulardii combined with Bifidobacterium and on cellular immune function in children with acute diarrhea.

Clinical effect of Saccharomyces boulardii combined with bifidobacterium and its effect on cellular immune function in children with acute diarrhea were studied. In total 116 cases of children with acute diarrhea admitted to Xuzhou Children's Hospital from March 2015 to March 2017 were collected and analyzed retrospectively. There were 59 children treated with Saccharomyces boulardii as control group and 57 children treated with Saccharomyces boulardii combined with bifidobacterium as experimental group. The clinical effect, stool frequency in different time periods, mean antidiarrheal time, mean antipyretic time and length of stay, and immune function of children in the two groups after treatment were analyzed. The cure rate (73.68%) and the total effective rate (87.72%) in the experimental group were significantly higher than those in the control group (47.46 and 71.19%) (P<0.05). The stool frequency in the experimental group was significantly lower than that in the control group 3 days after treatment (P<0.05). The mean antidiarrheal time in the experimental group was significantly shorter than that in the control group (P<0.05). The length of stay in the control group was significantly longer than that in the experimental group (P<0.05). CD3+, CD4+ and CD4+/CD8+ increased significantly in the experimental group after treatment while CD8+ decreased significantly (P<0.05). After treatment, the ratio of Th1 and Th2 in the two groups decreased significantly compared with before treatment (P<0.05), and the experimental group was significantly lower than the control group (P<0.05). After treatment, Th1/Th2 ratio was significantly higher than that before treatment (P<0.05), and the experimental group was significantly higher than the control group (P<0.05). In conclusion, treatment of acute diarrhea in children with Saccharomyces boulardii combined with bifidobacterium can effectively shorten the duration of diarrhea and hospital stay, reduce the number of diarrhea and enhance the cellular immune function.

Effects of Shenyankangfu tablets combined with losartan on cellular immune function, inflammatory factors and renal function in patients with chronic glomerulonephritis / 中国基层医药

Objective@#To investigate the effects of Shenyankangfu tablets combined with losartan on cellular immune function, inflammatory factors and renal function in patients with chronic glomerulonephritis.@*Methods@#From March 2017 to March 2018, 94 patients with chronic glomerulonephritis admitted to Hangzhou Dajiangdong Hospital were randomly divided into observation group (47 cases) and control group (47 cases). The control group was treated with losartan, and the observation group was treated with Shenyankangfu tablets on the basis of the control group.The two groups were treated for 12 weeks.The changes of cellular immune function, inflammatory factors and renal function were compared between the two groups before and after treatment.@*Results@#After treatment, the levels of CD4＋(71.98±3.28)%, CD4＋(40.01±3.86)% and CD4＋/CD8＋ (1.54±0.23) in the observation group were higher than those in the control group [(64.32±4.01)%, (34.87±1.96)% and (1.23±0.16)] (t=10.137, 8.140, 7.585, all P<0.05). After treatment, the level of IL-2 in the observation group [(3.18±0.59)μg/L] was higher than that in the control group [(2.37±0.71)μg/L], while the levels of IL-6[(227.42±12.41)ng/L] and TNF-α [(2.45±0.29)ng/L] in the observation group were lower than those in the control group [(289.64±19.36)ng/L and (3.38±0.65) ng/L] (t=6.015, 18.549, 8.958, all P<0.05). After treatment, the levels of Scr [(123.49±8.97)μmol/L], Upro[(0.76±0.18)g/24h] and BUN[(6.71±0.5)mmol/L] in the observation group were lower than those in the control group [(159.51±7.41)μmol/L, (1.28±0.23)g/24h, (7.91±0.47)mmol/L](t=21.224, 12.206, 11.136, all P<0.05). The total effective rate of the observation group (93.62%) was higher than that of the control group (72.34%) (P<0.05).@*Conclusion@#Shenyankangfu tablets combined with losartan can improve the cellular immune function and renal function in patients with chronic glomerulonephritis, and can reduce inflammatory reaction.The curative effect is remarkable, which is worthy of clinical reference.

Analysis of the characteristics of cellular immune function and its prognostic risk factors in patients with community-acquired pneumonia / 中华危重病急救医学

Objective To analyze the characteristics of cellular immune function and its prognostic risk factors in patients with community-acquired pneumonia (CAP). Methods The clinical data of patients diagnosed as CAP admitted to department of respiratory and critical care medicine of the Second Affiliated Hospital of Anhui Medical University from June 2018 to February 2019 were retrospectively analyzed. The patients were divided into survival group and death group according to the mortality at discharge and 28-day survival after hospital discharge; in addition, they were divided into bacterial group, fungi group and mixed infection group according to pathogen results at discharge. The differences of general clinical characteristics, arterial blood gas analysis indexes, plasma albumin, cellular immune function, inflammatory cytokines, the length of hospital stay among groups were analyzed. The correlation between the prognosis-related indicators in patients were analyzed by Pearson test or Spearman test, and Logistic regression model was used to analyze the risk factors of patients in non-survival group. Results 106 patients were finally enrolled, 69 of whom were survived, and 37 dead. Among 56 patients with pathogen results, 27 were diagnosed as bacterial infection, 11 as fungal infection, and 18 with mixed infection. Compared with the survival group, plasma albumin level, total T cell count, CD4+ T cell count, CD8+ T cell count were decreased in the death group, temperature, pH, acute physiology and chronic health evaluationⅡ(APACHEⅡ), procalcitonin (PCT), interleukin-6 (IL-6), C-reactive protein (CRP) were increased, and the length of hospital stay was significantly shortened. While there was no significant difference in gender, age, mean arterial pressure, arterial oxygen pressure, CD4+/CD8+, regulation T cell/effect T cell (TREG/TEF) between the two groups. Correlation analysis showed that plasma albumin, the length of hospital stay, APACHEⅡ score, CD4+ T cell count, CD8+ T cell count, CD4+/CD8+, PCT, IL-6, CRP were correlated with death (r value was -0.480, -0.209, 0.203, -0.279, -0.270, 0.271, 0.247, 0.410, 0.329, all P <0.05). Logistic regression analysis showed that plasma albumin, CD4+ T cell count, CD8+ T cell count, CD4+/CD8+decreased; APACHEⅡ score, PCT, IL-6, and CRP increased; the length of hospital stay were correlated with death, and all were independent risk factors for death in CAP patients (all P < 0.05). Correlation analysis showed that the APACHEⅡ score was negatively related to plasma albumin (r = -0.375, P < 0.05), positively related to CRP and IL-6 (r value was 0.363 and 0.333 respectively, both P < 0.05); negative correlation between plasma albumin and IL-6 (r = -0.372, P < 0.05), PCT and CD4+ T cell count (r = -0.354, P < 0.05), CRP and the length of hospital stay (r = -0.356, P < 0.05). There were no significant correlations between the others. There was no significant difference in cellular immune function or inflammatory factor expression between different pathogenic infections. Conclusions Cellular immune dysfunction, hypoproteinemia, APACHEⅡ score and elevated inflammatory index are all influential factors for the death of CAP patients. Apart from conventional anti-infective treatment, the symptoms of hypoproteinemia and cellular immune function can predict the severity and prognosis of CAP patients.

Effects of Shenyankangfu tablets combined with losartan on cellular immune function,inflammatory factors and renal function in patients with chronic glomerulonephritis / 中国基层医药

Objective To investigate the effects of Shenyankangfu tablets combined with losartan on cellular immune function,inflammatory factors and renal function in patients with chronic glomerulonephritis.Methods From March 2017 to March 2018,94 patients with chronic glomerulonephritis admitted to Hangzhou Dajiangdong Hospital were randomly divided into observation group (47 cases) and control group (47 cases).The control group was treated with losartan,and the observation group was treated with Shenyankangfu tablets on the basis of the control group .The two groups were treated for 12 weeks.The changes of cellular immune function ,inflammatory factors and renal function were compared between the two groups before and after treatment.Results After treatment, the levels of CD +4 (71.98 ±3.28)%,CD+4 (40.01 ±3.86)%and CD +4 ／CD+8 (1.54 ±0.23) in the observation group were higher than those in the control group [(64.32 ±4.01)%,(34.87 ±1.96)% and (1.23 ±0.16)] ( t ＝10.137,8.140, 7.585,all P＜0.05).After treatment,the level of IL－2 in the observation group [(3.18 ±0.59)μg／L] was higher than that in the control group [(2.37 ±0.71) μg／L],while the levels of IL －6 [(227.42 ±12.41) ng／L] and TNF－α[(2.45 ±0.29) ng／L] in the observation group were lower than those in the control group [(289.64 ± 19.36)ng／L and (3.38 ±0.65) ng／L] (t＝6.015,18.549,8.958,all P＜0.05).After treatment,the levels of Scr [(123.49 ±8.97) μmol／L],Upro[(0.76 ±0.18) g／24h] and BUN[(6.71 ±0.5) mmol／L] in the observation group were lower than those in the control group [(159.51 ±7.41) μmol／L, ( 1.28 ±0.23 ) g／24h, (7.91 ± 0.47)mmol／L]( t ＝21.224,12.206,11.136, all P ＜0.05).The total effective rate of the observation group (93.62%) was higher than that of the control group (72.34%) (P＜0.05).Conclusion Shenyankangfu tablets combined with losartan can improve the cellular immune function and renal function in patients with chronic glomerulone－phritis,and can reduce inflammatory reaction.The curative effect is remarkable ,which is worthy of clinical reference.

Effect of 3D laparoscopic hepatectomy in glissonian approach for hepatocellular carcinoma on the cellular immune function / 实用医学杂志

Objective To compare the effects of 3D laparoscopic hepatectomy (3D-LH) and open hepatectomy(OH)in glissonian access for hepatocellular carcinoma(HCC)on postoperative immunosuppression by analyzing cellular immune function. Methods During January 2014 to December 2017,sixty patients randomly scheduled to undergo hepatectomy were divided into 3D-LH group and OH group,with 30 patients in each group. All patients' peripheral blood was sampled to measure cell-mediated-immunologic markers(CD4+,CD8+T cell) before operation and in the mornings of the 1st,7thday after surgery. The effects of different operative methods on cellular immune function in patients organism were investigated. Results The decreased level in 3D-LH group was less than that in OH group in postoperative 1stday. The improvement in the 3D-LH group was more faster than that in the OH group on postoperative 7thday. There was statistical significance between the two groups(P<0.05), respectively. Conclusion When compared to open hepatectomy ,3D laparoscopy liver resection in glissonian approach for HCC,less effects in cellular immune function.

Effect of Feiliuping ointment combined with chemotherapy on cellular immune function and related inflammatory factors in patients with lung cancer / 中草药

Objective To study the effect of Feiliuping ointment combined with chemotherapy on the cellular immune function and related inflammatory factors in patients with lung cancer. Methods A total of 202 patients with non-small cell lung cancer (NSCLC) admitted to Henan Cancer Hospital from May 2014 to December 2016 were selected as subjects. They were randomly divided into the simple chemotherapy group (control group) and Feiliuping Ointment combined with chemotherapy group (observation group), and there were 101 cases in each group. The short-term curative effect, cellular immune function, and levels of inflammatory factors before and after treatment were observed and the adverse reactions were recorded. Results The effective rate and stability rate in the observation group were significantly higher than those in the control group (P < 0.05). Levels of CD3+, CD4+, CD4+/CD8+, and NK in the observation group after treatment were significantly higher than those in the control group (P < 0.05), while CD8+ was significantly lower than the control group (P < 0.05). Compared with the control group, the levels of serum IL-6, TNF-α, and hs-CPR were significantly lower in the observation group after treatment (P < 0.05). The incidence of adverse reactions in the observation group after treatment was significantly lower than that in the control group (P < 0.05). Conclusion Feiliuping Ointment combined with chemotherapy can effectively improve the clinical curative effect on lung cancer, regulate the immune mechanism, reduce the levels of inflammatory factors, improve the condition, and reduce the occurrence of adverse reactions in patients, thus deserving promoting.

Effect of different anesthetic methods on cellular immune functioning and the prognosis of patients with ovarian cancer undergoing oophorectomy.

The present study aimed to explore the effects of different anesthetic methods on cellular immune function and prognosis of patients with ovarian cancer (OC) undergoing oophorectomy. A total of 167 patients who received general anesthesia (GA) treatment (GA group) and 154 patients who received combined general/epidural anesthesia (GEA) treatment (GEA group) were collected retrospectively. Each group selected 124 patients that met the inclusion and exclusion criteria for further study. ELISA and radioimmunoassay were employed to detect levels of IL-2, TNF-α, and CA-125. The rates of tumor-red cell rosette (RTRR), red cell immune complex rosette (RRICR), and red cell C3b receptor rosette (RRCR) were also measured. Systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR) were determined by hemodynamics. The levels of tumor necrosis factor-α (TNF-α) and interleukin (IL)-2 decreased at 1 h intraoperation (T2), but increased 24-h post surgery (T3). The levels of TNF-α and IL-2 were recovered faster in the GEA group than in the GA group. The GA group exhibited greater levels of CA-125 expression than in the GEA group. The levels of RTRR, RRICR, and RRCR; ratios of CD3+, CD4+, CD4+/CD8+, CD16+, and CD56+ at 30 min after anesthesia (T1), T2, T3 and 48 h after the operation (T4) and levels of SBP, DBP, and HR at T1, T2, and T3 displayed increased levels in the GEA group than in the GA group. At 72-h post surgery (T5), the 5-year survival rate significantly increased in the GEA group compared with the GA group. GEA to be more suitable than GA for surgery on OC patients.

Correlation between circulating tumor cells EGFR expression and T cell subsets in advanced non-small cell lung cancer patients after tyrosine kinase inhibitor treatment.

Research indicates that after EGFR-tyrosine kinase inhibitors (EGFR-TKIs) treatment of non-small cell lung cancer (NSCLC), patient immune function significantly improved, and that circulating tumor cells (CTCs) measurements and peripheral blood epidermal growth factor receptor (EGFR) mutation data can guide TKIs treatment. Sixty-six advanced NSCLC patients treated with TKIs were enrolled and CTCs, EGFR expression, T cell subsets and natural killer (NK) cells in peripheral blood were measured using flow cytometry before and after treatment and assessed with respect to patient prognosis. CTCs and EGFR expression were negatively correlated with cellular immune function and immune recovery after EGFR-TKI treatment. Thus, CD4+/ CD8+ ratios and NK cells may be useful prognostic indicators for advanced NSCLC patients who receive TKIs treatment.

The clinical significance of thymopeptides α1 in the treatment of severe sepsis / 中国生化药物杂志

Objective To investigate the application values of thymosin α1 in severe sepsis.Methods Selected 94 patients with severe sepsis in our hospital from September 2014 to October 2016,were randomly divided into observation group(n=45)and control group(n=49),the control group was given routine treatment,the observation group was given thymosin α1 on the basis of conventional treatment,observed two groups before and after treatment T cell subsets and CD14+monocyte human leukocyte antigen(HLA-DR)and so on.Results The observation group the duration of ventilator use and ICU treatment were(12.51±3.82)d and(15.81±3.18)d,significantly shorter than the control group(P< 0.05).There was no significant difference in 28d mortality between the observation group and the control group; After treatment,the CD14+monocytes HLA-DR,CD3+and CD4+T cells in the observation group were significantly improved than before treatment(P<0.05);The HLA-DR,CD3+and CD4+T cells in the observation group after treatment were(36.04± 8.90)％,(58.93±8.74)％and(43.20±9.90)％,significantly higher than those in the control group(P<0.05);The observation group after treatment TNF-α,endotoxin and CRP respectively(56.40±11.78)ng/L,(27.83±9.98)ng/L and(53.20±9.73)g/mL,significantly lower than the control group(P< 0.05).Conclusion Application of thymosin α1 in the treatment of severe sepsis,which can improve the cellular immune function,adjust the state of inflammatory response,shorten the duration of mechanical ventilation and ICU stay.

Effect of Ningdong granule combined with haloperidol on the therapeutic effect and cellular immune function in children with Tourette's syndrome / 中国生化药物杂志

Objective To investigate the clinical effect and changes in cellular immune of children with Tourette 's syndrome in treatment with Ningdong granule and haloperidol.Methods 48 cases of children with Tourette's syndrome from February 2015 to February 2016 in hospital were selected and randomly divided into the observation group and control group with 24 cases in each group.The patients of two groups were all treated with conventional treatment, including mental relaxation, reverse muscle relaxation therapy and self-training.The patients of control group were treated with haloperidol, and on the basis of the control group, the patients of observation group were treated with Ningdong granule.The clinical effect, the levels of TNF-ɑ, IL-12, CD3, CD4, CD8, CD4/CD8 were compared before and after treatment between two groups, and recorded the incidence of adverse reactions in two groups of children.Results After the treatment, the total effective rate of the observation group was 91.6%, which was significantly better than the control group 66.6%, the difference was statistically significant (P<0.05).The levels of TNF-ɑ, IL-12, CD8 of the observation group were significantly lower than those of the control group (P<0.05), the levels of CD3, CD4, CD4/CD8 of the observation group were significantly higher than those of the control group, the difference was statistically significant (P<0.05).There were constipation, dizziness, drowsiness, fatigue, poor appetite, tachycardia and other adverse reactions occurred in both two groups, and the incidence of adverse reactions in the observation group was significantly lower than that in the control group, the difference was statistically significant (P<0.05).Conclusion The combination of Ningdong granule and haloperidol in the treatment of children with Tourette 's syndrome is effective and can improve the cellular immune function .

Static absorption compound anesthesia with intravenous anesthesia alone stress reaction and cellular immune function with gastric cancer surgery / 中国现代普通外科进展

Objective:To explore the analysis of the static absorption compound anesthesia with intravenous anesthesia alone stress reaction and cellular immune function in patients with gastric cancer surgery.Methods:From March 2014 to March 2014 were lines of gastric cancer patients,78 cases of elective surgical procedureS,using the random number table method was divided into control group and experimental group,each group of 39 cases,control group given simple intravenous anesthesia,patients given static absorption compound anesthesia,compared two groups of patients with preoperative 30 min (T0),cut the skin (T1),bi (T2),tube drawing immediate (T3),24 h after surgery (T4) different time points,such as serum C-reactive protein (CRP) and interleukin 2 (IL-2),the level of interleukin 8 (IL-8),CD4,CD8,the number of NK cells,and CD4/CD8 ratio.Re· sults:T2 IL-2,IL-8 point test group dropped to the lowest level,CRP rose to the highest,to T4 point back to the level before anesthesia,IL-2 and the control group,while the level of IL-8 T3 point minimum,CRP rose to the highest,and T4 point is still not fully recovered to the level before anesthesia,CRP T1 and T4 each point test group,IL-2 and IL-8 cytokine levels were significantly better than the control group,with significant difference (P＜0.05).T1 point,two groups of patients with CD4,NK cells and CD4/CD8 ratios were significantly decreased,CD8 levels increased significantly,CD4 T3 point test group began to rebound,NK cells and CD4/CD8 ratio,level of CD8 began to decline,and T4 point before anesthesia recovery level,and T4 point control indicators are still relatively preoperative levels have obvious deviation,T1 and T4 each point group CD4,NK cells and CD4/CD8 ratio than the control group obviously on the higher side,had clear statistical significance(P＜0.05),and each point CD8 levels have no obvious difference between groups(P＞0.05).Conelusions:Different anesthetic methods on gastric cancer surgery the patient's level of stress reaction and cell immune function all can produce certain effect,compared with the conventional simple intravenous anesthesia,the static absorption compound anesthesia can obviously relieve open perioperative stress response in patients with gastric cancer,reduce the inhibition of cellular immune level,is a kind of can effectively guarantee the anesthesia effect and prognosis of patients with ideal anesthesia method.

Effects of pegylated interferon on cellular immune function in patients with chronic hepatitis C / 中国生化药物杂志

Objective To investigate the clinical effect of pegylated interferon(IFN)on chronic hepatitis C and its effect on cellular immune function.Methods A total of 92 patients with chronic hepatitis C were enrolled in our hospital from June 2013 to October 2016.They were randomly divided into study group and control group by single and double numbers(admission order),with 46 cases in each group.The control group was treated with recombinant human interferon alpha-2b on the basis of conventional treatment.The study group was treated with pegylated interferon alpha-2a on the basis of conventional treatment.Results After treatment, the rates of RVR, EVR, ETVR and SVR in the study group(52.17%, 71.74%, 76.09% and 54.35%, respectively)were significantly higher than those in the control group(34.78%, 50%, 58.70%, 39.13%, respectively), The difference was statistically significant(P<0.05).There were no significant difference in the cellular immune function index between the two groups before treatment;After treatment,the CD3+, CD4+, CD4+/CD8+ and other immune function indexes of the study group were significantly higher than those before treatment, while the results of CD8+ were significantly lower than before treatment(P<0.05);There was no significant difference in the cellular immune function in the control group compared with before, and the difference of the cellular immune function between the 2 groups after treatment was statistically significant(P<0.05).Conclusion Pegylated interferon(IFN)on the basis of routine treatment can improve the virologic response and cellular immune function in patients with chronic hepatitis C.

Effects of Different Anesthetic Methods on Cellular Immune and Neuroendocrine Functions in Patients With Hepatocellular Carcinoma Before and After Surgery.

BACKGROUND: Many anesthesia methods have been studies in hepatocellular carcinoma (HCC). We aimed to explore the effects of combined intravenous and inhalation anesthesia and combined general and epidural anesthesia on cellular immune function and neuroendocrine function in patients with HCC before and after surgery. METHODS: Between September 2012 and April 2014, 72 patients who underwent a hepatectomy in our hospital were enrolled. RESULTS: Compared with the combined intravenous and inhalation anesthesia group, the combined general and epidural anesthesia group demonstrated increased CD4+ /CD8+ T cells 0 hr after surgery, increased CD3+ , CD4+ , CD4+ /CD8+ cells, and IFN-γ levels 12 hr after surgery, and increased CD3+ , CD4+ , and CD4+ /CD8+ cells 24 hr after surgery (all P < 0.05). At 72 hr after surgery, the levels of ACTH and Cor in the combined general and epidural anesthesia group, and the levels of CD3+ , CD4+ , CD4+ /CD8+ cells, and IFN-γ in both the combined intravenous and inhalation anesthesia and the combined general and epidural anesthesia groups decreased to pre-surgery levels. Significant differences were observed in the comparisons of CD3+ , IL-6, and IL-10 between the combined intravenous and inhalation anesthesia and the combined general and epidural anesthesia groups 72 hr after surgery (all P < 0.05). CONCLUSION: Our results revealed that combined general and epidural anesthesia plays a crucial role in hepatectomy via the mitigation of the inhibition of immunologic function in HCC patients during the perioperative period. Combined general and epidural anesthesia also hastens the recovery of immunologic suppression after surgery, which can provide a certain reference for the selection of clinical anesthesia in the treatment of HCC.