Primed and cued: long-term acoustic telemetry links interannual and seasonal variations in freshwater flows to the spawning migrations of Common Snook in the Florida Everglades.

BACKGROUND: Spawning migrations are a widespread phenomenon among fishes, often occurring in response to environmental conditions prompting movement into reproductive habitats (migratory cues). However, for many species, individual fish may choose not to migrate, and research suggests that conditions preceding the spawning season (migratory primers) may influence this decision. Few studies have provided empirical descriptions of these prior conditions, partly due to a lack of long-term data allowing for robust multi-year comparisons. To investigate how primers and cues interact to shape the spawning migrations of coastal fishes, we use acoustic telemetry data from Common Snook (Centropomus undecimalis) in Everglades National Park, Florida, USA. A contingent of Snook migrate between rivers and coastal spawning sites, varying annually in both the proportion of the population that migrates and the timing of migration within the spawning season. However, the specific environmental factors that serve as migratory primers and cues remain unknown. METHODS: We used eight years of acoustic telemetry data (2012-2019) from 173 tagged Common Snook to investigate how primers and cues influence migratory patterns at different temporal scales. We hypothesize that (1) interannual differences in hydrologic conditions preceding the spawning season contribute to the number of individuals migrating each year, and (2) specific environmental cues trigger the timing of migrations during the spawning season. We used GLMMs to model both the annual and seasonal migratory response in relation to flow characteristics (water level, rate of change in water level), other hydrologic/abiotic conditions (temperature, salinity), fish size, and phenological cues independent of riverine conditions (photoperiod, lunar cycle). RESULTS: We found that the extent of minimum marsh water level prior to migration and fish size influence the proportion of Snook migrating each year, and that high river water level and daily rates of change serve as primary cues triggering migration timing. CONCLUSION: Our findings illustrate how spawning migrations are shaped by environmental factors acting at different temporal scales and emphasize the importance of long-term movement data in understanding these patterns. Research providing mechanistic descriptions of conditions that promote migration and reproduction can help inform management decisions aimed at conserving ecologically and economically important species.

Variability in nearshore fish biodiversity indicators after a mining disaster in eastern Brazil.

The rupture of the Fundão mining dam (Doce river basin, Brazil) caused a wide range of negative impacts. Yet, assemblage-level implications to estuarine and coastal fishes remain unclear, partly due to the lack of pre-disaster information. Based on monthly otter trawl surveys, we analyzed spatial and seasonal variability in univariate (total biomass, biomass of species vulnerable to exploitation, rarefied richness and evenness) and multivariate (species composition and trophic composition) indicators of fish biodiversity in the Doce river delta, eastern Brazil. We determined the independent and interactive effects of environmental, seasonal and spatial variables on species composition to test whether environmental alterations provoked by mine tailings could affect assemblage's organization. Most indicators present idiosyncratic spatiotemporal patterns, suggesting they have complementary roles in revealing changes in fish biodiversity. Environmental variables, including those affected by the Fundão dam collapse such as turbidity, dissolved oxygen and pH, were much more important than seasonal and spatial predictors in explaining the variation in fish species composition. These findings highlight the potential from mine tailings to disrupt local ichthyofauna and indicate a preponderant role of environmental conditions in assemblage structuring. Given the lack of data prior to rupture, our results may be used as a baseline against which to assess temporal trends in fish biodiversity relative to changes detected in less disturbed estuarine and coastal assemblages.

A multidisciplinary analytical framework to delineate spawning areas and quantify larval dispersal in coastal fish.

Assessing larval dispersal is essential to understand the structure and dynamics of marine populations. However, knowledge about early-life dispersal is sparse, and so is our understanding of the spawning process, perhaps the most obscure component of biphasic life cycles. Indeed, poorly known species-specific spawning modality and species-specific early-life traits, as well as the high spatio-temporal variability of the oceanic circulation experienced during larval drift, hamper our ability to appraise the realized connectivity of coastal fishes. Here, we propose an analytical framework which combines Lagrangian modelling, network theory, otolith analyses and biogeographical information to pinpoint and characterize larval sources which are then grouped into discrete spawning areas. Such well-delineated larval sources allow improving the quantitative evaluations of both dispersal scales and connectivity patterns. To illustrate its added value, our approach is applied to two case-studies focusing on Diplodus sargus and Diplodus vulgaris in the Adriatic sea. We evidence robust correlations between otolith geochemistry and modelled spawning areas to assess their relative importance for the larval replenishment of the Apulian coast. Our results show that, contrary to D. sargus, D. vulgaris larvae originate from both eastern and western Adriatic shorelines. Our findings also suggest that dispersal distances and dispersal surfaces scale differently with the pelagic larval duration. Furthermore, 30.8% of D. sargus larvae and 23.6% of D. vulgaris larvae of the Apulian populations originate from Marine protected area (MPA), exemplifying larval export from MPAs to surrounding unprotected areas. This flexible multidisciplinary framework, which can be adjusted to any coastal fish and oceanic system, exploits the explanatory power of a dispersal model, fine-tuned and backed-up by observations, to provide more reliable scientific basis for the management and conservation of marine ecosystems.

Divergent transcriptomic signatures in response to salinity exposure in two populations of an estuarine fish.

In estuary and coastal systems, human demand for freshwater, climate change-driven precipitation variability, and extreme weather impact salinity levels, reducing connectivity between mesohaline coastal fish populations and potentially contributing to genomic divergence. We examined gill transcriptome responses to salinity in wild-caught juveniles from two populations of Sacramento splittail (Pogonichthys macrolepidotus), a species of conservation concern that is endemic to the San Francisco Estuary, USA, and the lower reaches of its tributaries. Recent extreme droughts have led to salinities above the tolerance limits for this species, creating a migration barrier between these populations, which potentially contributed to population divergence. We identified transcripts involved in a conserved response to salinity; however, the more salinity-tolerant San Pablo population had greater transcriptome plasticity (3.6-fold more transcripts responded than the Central Valley population) and a response consistent with gill remodeling after 168 hr of exposure to elevated salinity. The reorganization of the gill in response to changing osmotic gradients is a process critical for acclimation and would facilitate enhanced salinity tolerance. We detected an upregulation of receptors that control the Wnt (wingless-type) cell signaling pathway that may be required for an adaptive response to increases in salinity, patterns not observed in the relatively salinity-sensitive Central Valley population. We detected 62 single nucleotide polymorphisms (SNPs) in coding regions of 26 transcripts that differed between the populations. Eight transcripts that contained SNPs were associated with immune responses, highlighting the importance of diversity in immune gene sequences as a defining characteristic of genomic divergence between these populations. Our data demonstrate that these populations have divergent transcriptomic responses to salinity, which is consistent with observed physiological differences in salinity tolerance.

Using DNA Barcodes to Aid the Identification of Larval Fishes in Tropical Estuarine Waters (Malacca Straits, Malaysia).

Larval descriptions of tropical marine and coastal fishes are very few, and this taxonomic problem is further exacerbated by the high diversity of fish species in these waters. Nonetheless, accurate larval identification in ecological and early life history studies of larval fishes is crucial for fishery management and habitat protection. The present study aimed to evaluate the usefulness of DNA barcodes to support larval fish identification since conventional dichotomous keys based on morphological traits are not efficient due to the lack of larval traits and the rapid morphological changes during ontogeny. Our molecular analysis uncovered a total of 48 taxa (21 families) from the larval samples collected from the Klang Strait waters encompassing both spawning and nursery grounds of marine and estuarine fishes. Thirty-two (67%) of the larval taxa were identified at the species level, two taxa (4%) at the genus level, and 14 taxa (29%) at family level. The relatively low rate of species-level identification is not necessarily due to the DNA barcoding method per se, but a general lack of reference sequences for speciose and non- commercial fish families such as Gobiidae, Blenniidae, and Callionymidae. Larval morphology remains important in species diagnoses when molecular matches are ambiguous. A lower ethanol percentage (50%) for larva preservation is also useful to keep the body of larvae intact for morphological identification, and to preserve DNA for subsequent molecular analyses. The 10% Chelex resin used to extract DNA is also cost- effective for long term monitoring of larval fishes. Hence, the DNA barcoding method is an effective and easy way to aid the identification of estuarine larval fishes at the species level.

Taxonomic and functional distinctness of the fish assemblages in three coastal environments (bays, coastal lagoons and oceanic beaches) in Southeastern Brazil.

Several species of marine fish use different coastal systems especially during their early development. However, these habitats are jeopardized by anthropogenic influences threatening the success of fish populations, and urgent measures are needed to priorize areas to protect their sustainability. We applied taxonomic (Δ+) and functional (X+) distinctiveness indices that represent taxonomic composition and functional roles to assess biodiversity of three different costal systems: bays, coastal lagoons and oceanic beaches. We hypothesized that difference in habitat characteristics, especially in the more dynamism and habitat homogeneity of oceanic beaches compared with more habitat diversity and sheltered conditions of bays and coastal lagoons results in differences in fish richness and taxonomic and functional diversity. The main premise is that communities phylogenetically and functionally more distinct have more interest in conservation policies. Significant differences (P < 0.004) were found in the species richness, Δ+ and X+ among the three systems according to PERMANOVA. Fish richness was higher in bays compared with the coastal lagoons and oceanic beaches. Higher Δ+ was found for the coastal lagoons compared with the bays and oceanic beaches, with the bays having some values below the confidence limit. Similar patterns were found for X+, although all values were within the confidence limits for the bays, suggesting that the absence of some taxa does not interfere in functional diversity. The hypothesis that taxonomic and functional structure of fish assemblages differ among the three systems was accepted and we suggest that coastal lagoons should be priorized in conservation programs because they support more taxonomic and functional distinctiveness.

Age and growth of the white croaker Micropogonias furnieri (Perciformes: Sciaenidae) in a coastal area of Southeastern Brazilian Bight

Studies on age and growth of fish populations have direct application in fisheries and contribute for policies of conservation. Our aim was update information on the Micropogonias furnieri growth parameters based on sagittae otoliths annulis readings, and we expected that the studied population from Southeast Brazil can reach more longevity than the reported in the available literature. We examined 286 individuals between August-2010 and July-2011 from Ubatuba Bay, SP. The Total Length ranged 200-480 mm. The determined growth parameters were: Males, L∞ =523.4mm, k = 0.05×year-1, t0 = -8.78 year; Females L∞ = 573.5mm, k = 0.06 × year-1, t0 = -7.49 year. The MI and % of edge type evidenced the formation of one ring per year during autumn/winter, coinciding with low temperature and low growth rate. These results differed from those reported for the Vazzoler’s Population I (summer), and we detected higher longevity (45-48 years) as compared with the recorded in the available information.(AU)

Estudos de idade e crescimento de peixes têm aplicação direta na biologia pesqueira e contribuem para medidas de conservação. Nosso objetivo foi atualizar informações sobre parâmetros de crescimento de Micropogonias furnieri utilizando leitura de anéis etários em otólitos sagittae, esperando que a população do Sudeste do Brasil alcance maior longevidade do que o reportado na literatura. Examinamos 286 indivíduos, capturados entre agosto de 2010 e julho de 2011, na Baía de Ubatuba, SP. O comprimento total variou de 200-480 mm. Foram determinados os parâmetros de crescimento: Machos, L∞ = 523,4 mm, k = 0,05 ano-1, t0 = -8,78 ano; Fêmeas, L∞ = 573,5 mm, k = 0,06 ano-1, t0 = -7,49 ano. O IM e a porcentagem do tipo de borda evidenciaram o padrão de formação anual de anéis no outono/inverno, coincidindo com mais baixas temperaturas e crescimento lento. Estes resultados diferem dos reportados para População I de Vazzoler (verão), e nós detectamos longevidade bem superior (45-48 anos) à descrita anteriormente.(AU)