Nutrient intake, productive and metabolic parameters of Nellore bulls feed a forage free diet using cottonseed cake as a fiber source.

We hypothesized that cottonseed cake in confined Nellore young bulls' diet as fiber source, could maintain or improve the nutrient intake, and productive and metabolic parameters. It was evaluated the total replacement of whole plant corn silage (WPCS) by cottonseed cake (CSC) as a source of fiber in the diet. A completely randomized design with two treatments and 12 replications was used. The treatments were two experimental diets containing 300 g/kg of WPCS or CSC as roughage. All animals at 0, 15, 30, 60 and 112 days were weighed to monitor productive performance and nutrient intake. Dry matter intakes, organic matter, neutral detergent fiber, physically effective neutral detergent fiber, crude protein and non-fiber carbohydrates of the WPCS treatment cattle were higher (p < 0.05), and the ethereal extract intake of cattle fed CSC diet was higher. The BW of cattle fed WPCS was higher in the two initial periods of confinement (p < 0.05). Animals from both treatments showed linear growth during the confinement period. There was a fiber source × period interaction for total weight gain (TWG), average daily gain (ADG) and feed efficiency (FE) (p < 0.05), with the lowest in the first 15 days and the highest at 30 days, reflecting a compensatory gain in animals fed with CSC. Bulls fed with CSC showed negative TWG and ADG in the first period (p < 0.05), but recovered in the following periods, surpassing that of the SC treatment in the last two periods. There was negative EF of bulls fed with CSC in the first period (P < 0.05), but in the following periods the CSC diet did not differ from the WPCS diet. The animals fed CSC diet showed a higher concentration of cholesterol and glucose in the blood plasma (p < 0.05). The forage-free diet containing cottonseed cake as a source of fiber replacing corn silage promoted better animal performance.

Ruminal responses, digestibility, and blood parameters of beef cattle fed diets with different oilseeds.

It aimed to evaluate the effects of different whole oilseeds in lipid-rich diets on nutrient intake, apparent digestibility, ingestive behavior, and ruminal and blood parameters of steers. A control diet (without oilseed) and four diets containing whole oilseeds (cotton, canola, sunflower, and soybean) were tested. All diets used the whole-plant corn silage at 400 g/kg as roughage. Five diets, being a control diet (without oilseed) and four diets containing whole oilseeds (cotton, canola, sunflower, and soybean), were tested. All diets used the whole-plant corn silage at 400 g/kg as roughage. Five rumen fistulated crossbreed steers, in a 5 × 5 Latin square design were distributed using five periods of 21 days. The steers fed cottonseed and canola diets had lower dry matter intakes (6.6 kg/day). Steers showed higher averages of time in rumination for treatments with sunflower, soybean, and cottonseed (406, 362, and 361 min/day, respectively). There was no treatment effect for the ruminal pH and ammonia (NH3) variables. There was an effect of the treatment on the volatile fatty acid concentrations. The animals that received soybean showed a higher plasma urea concentration (50.7 mg/dL). Animals fed the control diet showed lower serum cholesterol levels (111.8 mg/dL) than those fed diets containing whole cottonseed, canola, sunflower, and soybean (152.7, 137.1, 146.9, and 138.2 mg/dL, respectively). We recommended using whole soybean or sunflower seeds to formulate lipid-rich diets with 70 g/kg of ether extract for crossbreed steers in the feedlot.

Development of a new generation of miniemulsion based on cottonseed oil with α-tocopherol and ZnO and evaluation of its adjuvant activity.

Background: Emulsions have been widely used as immunological adjuvants. But the use of materials derived from plants such as cottonseed oil, alpha-tocopherol, or minerals such as zinc, as well as their use at the nanometric scale has been little explored. In this study, we develop a new miniemulsion and evaluated its antioxidant and phagocytic capacity, as well as parameters related to immune response stimulation by cytokine expression and antibodies production in a mice model. Methods: Formulated CN (cottonseed oil miniemulsion) and CNZ (cottonseed oil miniemulsion whit zinc oxide nanoparticles) miniemulsions were characterized by scanning electronic microscopy SEM, DLS and FT-IR. In murine macrophages, splenocytes and thymocytes primary cultures safety and cytotoxicity were determined by MTT. In macrophages the antioxidant and phagocytic capacity was evaluated. In BALB/c mice, the stimulation of the immune system was determined by the expression of cytokines and the production of antibodies. Results: The CN and CNZ presented stability for 90 days. Immediately after preparation, the CN presented a higher particle size (543.1 nm) than CNZ (320 nm). FT-IR demonstrated the correct nanoparticle synthesis by the absence of sulfate groups. CN and CNZ (1.25 to 10 µL/mL) had no toxic effect on macrophages (p = 0.108), splenocytes (p = 0.413), and thymocytes (p = 0.923). All CN and CNZ doses tested induced nitric oxide and antioxidants production in dose dependent manner when compared with control. CN-ovalbumin and CNZ-ovalbumin treatments in femoral subcutaneous tissue area showed inflammation with higher leukocyte infiltration compared with FCA. The intraperitoneal administration with CN, CNZ, and FCA showed a higher total intraperitoneal cells recruitment (CD14+) after 24 h of inoculation than control (p = 0.0001). CN and CNZ increased the phagocyte capacity with respect to untreated macrophages in the Candida albicans-phagocytosis assay. The evaluation of residual CFU indicated that only CN significantly decreased (p = 0.004) this value at 3 h. By other side, only CN increased (p = 0.002) the nitric oxide production. CNZ stimulated a major INFγ secretion compared with FCA at day 7. A major IL-2 secretion was observed at days 7 and 14, stimulated with CN and CNZ. Both miniemulsions did not affect the antibody isotypes production (IgG1, IgG2a, IgG3, IgA and IgM) at days 7, 14, 28, and 42. CN induced a significant IgG production against OVA, but lesser than FCA. Conclusions: The two new miniemulsions with adjuvant and antioxidant capacity, were capable of generating leukocyte infiltration and increased cytokines and antibodies production.

Bioactives and Extracellular Enzymes Obtained from Fermented Macrofungi Cultivated in Cotton and Jatropha Seed Cakes.

This work focused on obtaining fermented oil cake (cotton or Jatropha) via macrofungi growth with potential characteristics for animal feed formulations, such as the presence of extracellular enzymes, bioactive (ergosterol and antioxidants), and detoxification of antinutritional compounds. The concentration of phorbol esters was reduced by four macrofungi in Jatropha seed cake (JSC) to non-toxic levels. At least two macrofungi efficiently degraded free gossypol in cottonseed cake (CSC). Fermentation with Coriolopsis sp. INPA1646 and Tyromyces sp. INPA1696 resulted in increased ergosterol concentrations, antioxidant activity reduction, and high activity of laccases and proteases. Bromatological analysis indicated high crude protein concentrations, with partial solubilization by fungal proteases. Fermented products from Coriolopsis sp. and Tyromyces sp. in JSC or CSC can be considered important biological inputs for monogastric and polygastric animal feed.

Physicochemical features, functional characteristics, and health benefits of cottonseed oil: a review / Benefícios à saúde humana e características físico-químicas e funcionais do óleo de semente de algodão: uma revisão

Vegetable oils have their specific physicochemical properties due to which they are playing vital role in human nutritional diet for health benefits. Cottonseed oil is obtained from various species of cotton seeds that are famous to be grown mainly for their fiber quality. The most prominently used specie is Gossypium hirsutum. It is obvious that the seeds of different variety of cotton vary as grown in diverse agroclimatic conditions with respect to oil, fats and protein contents. Cottonseed oil is routinely used for cooking and food manufacturing products. Cottonseed oil obtained after proper extraction/processing steps from crude state to refined oil in a variety of ways. Cotton crop is considered for their dual-use purpose, for fiber quality and oil production to promote health benefits in the world. Keeping in view the above facts, this review clearly demonstrated an overview about physicochemical and functional properties of cottonseed oil to promote health benefits associated with the use of this oil. The overall characteristics and all concerned health benefits of CSO will further improve their usefulness is a compact way. We have summarized a brief multi-dimensional features of CSO in all aspects up to the best of our knowledge fort he end researchers who can further research in the respective aspect.(AU)

Os óleos vegetais têm propriedades físico-químicas específicas que desempenham um papel vital na dieta nutricional humana em benefício à saúde. O óleo de semente de algodão, utilizado rotineiramente no preparo e na fabricação de alimentos, é obtido através de várias espécies de sementes de algodão, famosas pela alta qualidade de sua fibra, cuja espécie mais utilizada é Gossypium hirsutum. As sementes variam, em relação ao teor do óleo, da gordura e das proteínas, de acordo com o cultivo e as diversas condições agroclimáticas. O óleo de algodão é obtido após etapas adequadas de extração das sementes e processamento do estado bruto ao refinado. Assim, a presente revisão demonstra, de maneira geral, as propriedades físico-químicas e funcionais do óleo de semente de algodão e seus benefícios à saúde humana, resumindo suas características multidimensionais. As características gerais e todos os benefícios do composto podem melhorar ainda mais se utilizadas de forma compacta, auxiliando futuros pesquisadores.(AU)

Physicochemical features, functional characteristics, and health benefits of cottonseed oil: a review / Benefícios à saúde humana e características físico-químicas e funcionais do óleo de semente de algodão: uma revisão

Vegetable oils have their specific physicochemical properties due to which they are playing vital role in human nutritional diet for health benefits. Cottonseed oil is obtained from various species of cotton seeds that are famous to be grown mainly for their fiber quality. The most prominently used specie is Gossypium hirsutum. It is obvious that the seeds of different variety of cotton vary as grown in diverse agroclimatic conditions with respect to oil, fats and protein contents. Cottonseed oil is routinely used for cooking and food manufacturing products. Cottonseed oil obtained after proper extraction/processing steps from crude state to refined oil in a variety of ways. Cotton crop is considered for their dual-use purpose, for fiber quality and oil production to promote health benefits in the world. Keeping in view the above facts, this review clearly demonstrated an overview about physicochemical and functional properties of cottonseed oil to promote health benefits associated with the use of this oil. The overall characteristics and all concerned health benefits of CSO will further improve their usefulness is a compact way. We have summarized a brief multi-dimensional features of CSO in all aspects up to the best of our knowledge fort he end researchers who can further research in the respective aspect.

Os óleos vegetais têm propriedades físico-químicas específicas que desempenham um papel vital na dieta nutricional humana em benefício à saúde. O óleo de semente de algodão, utilizado rotineiramente no preparo e na fabricação de alimentos, é obtido através de várias espécies de sementes de algodão, famosas pela alta qualidade de sua fibra, cuja espécie mais utilizada é Gossypium hirsutum. As sementes variam, em relação ao teor do óleo, da gordura e das proteínas, de acordo com o cultivo e as diversas condições agroclimáticas. O óleo de algodão é obtido após etapas adequadas de extração das sementes e processamento do estado bruto ao refinado. Assim, a presente revisão demonstra, de maneira geral, as propriedades físico-químicas e funcionais do óleo de semente de algodão e seus benefícios à saúde humana, resumindo suas características multidimensionais. As características gerais e todos os benefícios do composto podem melhorar ainda mais se utilizadas de forma compacta, auxiliando futuros pesquisadores.

Physicochemical features, functional characteristics, and health benefits of cottonseed oil: a review

Abstract Vegetable oils have their specific physicochemical properties due to which they are playing vital role in human nutritional diet for health benefits. Cottonseed oil is obtained from various species of cotton seeds that are famous to be grown mainly for their fiber quality. The most prominently used specie is Gossypium hirsutum. It is obvious that the seeds of different variety of cotton vary as grown in diverse agroclimatic conditions with respect to oil, fats and protein contents. Cottonseed oil is routinely used for cooking and food manufacturing products. Cottonseed oil obtained after proper extraction/processing steps from crude state to refined oil in a variety of ways. Cotton crop is considered for their dual-use purpose, for fiber quality and oil production to promote health benefits in the world. Keeping in view the above facts, this review clearly demonstrated an overview about physicochemical and functional properties of cottonseed oil to promote health benefits associated with the use of this oil. The overall characteristics and all concerned health benefits of CSO will further improve their usefulness is a compact way. We have summarized a brief multi-dimensional features of CSO in all aspects up to the best of our knowledge for the end researchers who can further research in the respective aspect.

Resumo Os óleos vegetais têm propriedades físico-químicas específicas que desempenham um papel vital na dieta nutricional humana em benefício à saúde. O óleo de semente de algodão, utilizado rotineiramente no preparo e na fabricação de alimentos, é obtido através de várias espécies de sementes de algodão, famosas pela alta qualidade de sua fibra., cuja espécie mais utilizada é Gossypium hirsutum. As sementes variam, em relação ao teor do óleo, da gordura e das proteínas, de acordo com o cultivo e as diversas condições agroclimáticas. O óleo de algodão é obtido após etapas adequadas de extração das sementes e processamento do estado bruto ao refinado. Assim, a presente revisão demonstra, de maneira geral, as propriedades físico-químicas e funcionais do óleo de semente de algodão e seus benefícios à saúde humana, resumindo suas características multidimensionais. As características gerais e todos os benefícios do composto podem melhorar ainda mais se utilizadas de forma compacta, auxiliando futuros pesquisadores.

Physicochemical features, functional characteristics, and health benefits of cottonseed oil: a review / Benefícios à saúde humana e características físico-químicas e funcionais do óleo de semente de algodão: uma revisão

Vegetable oils have their specific physicochemical properties due to which they are playing vital role in human nutritional diet for health benefits. Cottonseed oil is obtained from various species of cotton seeds that are famous to be grown mainly for their fiber quality. The most prominently used specie is Gossypium hirsutum. It is obvious that the seeds of different variety of cotton vary as grown in diverse agroclimatic conditions with respect to oil, fats and protein contents. Cottonseed oil is routinely used for cooking and food manufacturing products. Cottonseed oil obtained after proper extraction/processing steps from crude state to refined oil in a variety of ways. Cotton crop is considered for their dual-use purpose, for fiber quality and oil production to promote health benefits in the world. Keeping in view the above facts, this review clearly demonstrated an overview about physicochemical and functional properties of cottonseed oil to promote health benefits associated with the use of this oil. The overall characteristics and all concerned health benefits of CSO will further improve their usefulness is a compact way. We have summarized a brief multi-dimensional features of CSO in all aspects up to the best of our knowledge for the end researchers who can further research in the respective aspect.

Os óleos vegetais têm propriedades físico-químicas específicas que desempenham um papel vital na dieta nutricional humana em benefício à saúde. O óleo de semente de algodão, utilizado rotineiramente no preparo e na fabricação de alimentos, é obtido através de várias espécies de sementes de algodão, famosas pela alta qualidade de sua fibra., cuja espécie mais utilizada é Gossypium hirsutum. As sementes variam, em relação ao teor do óleo, da gordura e das proteínas, de acordo com o cultivo e as diversas condições agroclimáticas. O óleo de algodão é obtido após etapas adequadas de extração das sementes e processamento do estado bruto ao refinado. Assim, a presente revisão demonstra, de maneira geral, as propriedades físico-químicas e funcionais do óleo de semente de algodão e seus benefícios à saúde humana, resumindo suas características multidimensionais. As características gerais e todos os benefícios do composto podem melhorar ainda mais se utilizadas de forma compacta, auxiliando futuros pesquisadores.

Cottonseed and Soy-bean agro-industrial by-products used in feedlot cattle diet: effects on beef fatty acid profile and quality parameters / Subprodutos agroindustriais de caroço de algodão e soja utilizados na dieta de bovinos confinados: efeitos no perfil de ácidos graxos e parâmetros de qualidade da carne bovina

Beef composition are directly influenced by the dietary fat, particularly in ruminants, due to rumen biohydrogenation, which promotes alterations in the dietary fatty acid (FA) profile and affects the meat quality. Beef sensorial, chemical, and FA compositions of intramuscular and subcutaneous fat were evaluated, from 40 Nellore males finished on feedlot diet based, containing cottonseed (CSB) and soybean by-product (SOB) as agro-industrial by-product fat sources. CSB as a fat source, did not alter the beef pH, shear force, chemical composition, or subcutaneous FA profile compared with the SOB diet. Differences were observed at yellow and red beef color, with low and high CSB diet inclusion; on the contrary, inclusion of SOB in the diet led to an intensely unpleasant aroma in aged and cooked meats. Regarding beef FA profile, CSB with 3% dietary fat produced steaks with a lower proportion of C 17:0 , having 1.359 and 3.238 g/100 g of intramuscular FA, whereas CSB with 5% dietary fat led to an increase in C 18:2 n -6 , with 0.298 and 0.132 g/100 g of intramuscular FA in steaks from animals fed with CSB and SOB, respectively. CSB produced more intense red beef color, unpleasant aroma, and higher linoleic acid content; however, the color and aroma of the meat produced from cattle fed with CSB in the diet were less intense and were like those observed in cattle fed with SOB; these could therefore be recommended for use as agro-industrial by-products in beef cattle diet.(AU)

A composição da carne bovina é influenciada diretamente pela gordura da dieta, principalmente em ruminantes, devido à biohidrogenação ruminal, que promove alterações no perfil de ácidos graxos dietéticos e afeta a qualidade da carne. Foram avaliadas às composições sensoriais, químicas e de ácido graxo da carne, da gordura intramuscular e subcutânea, de 40 machos Nelore terminados em dieta de confinamento contendo caroço de algodão (CSB) e subproduto de soja (SOB) como fontes de gordura de subprodutos agroindustriais. CSB, não alterou o pH da carne, força de cisalhamento, composição química ou perfil de ácido graxo subcutâneo em comparação com a dieta SOB. Diferenças foram observadas na cor amarela e vermelha da carne bovina, com baixa e alta inclusão de CSB na dieta; a inclusão de SOB na dieta levou a um aroma intensamente desagradável em carnes envelhecidas e cozidas. Em relação ao perfil de ácido graxo da carne bovina, CSB com 3% de gordura dietética produziu bifes com menor proporção de C 17: 0 , tendo 1.359 e 3.238 g / 100 g de ácido graxo intramuscular, enquanto CSB com 5% de gordura dietética levou a um aumento de C 18: 2n- 6 , com 0,298 e 0,132 g / 100 g de ácido graxo intramuscular em bifes de animais alimentados com CSB e SOB, respectivamente. CSB produziu cor vermelha bovina mais intensa, aroma desagradável e maior teor de ácido linoléico; entretanto, a cor e o aroma da carne produzida em bovinos alimentados com CSB na dieta foram menos intensos e semelhantes aos observados em bovinos alimentados com SOB; estes poderiam, portanto, ser recomendados para uso como subprodutos agroindustriais na dieta de bovinos de corte.(AU)

Long-Term and Carryover Effects of Supplementation with Whole Oilseeds on Methane Emission, Milk Production and Milk Fatty Acid Profile of Grazing Dairy Cows.

Research is ongoing to find nutritional methane (CH4) mitigation strategies with persistent effects that can be applied to grazing ruminants. Lipid addition to dairy cow diets has shown potential as means to decrease CH4 emissions. This study evaluated the effects of oilseeds on CH4 emission and production performance of grazing lactating dairy cows. Sixty Holstein Friesian cows grazing pasture were randomly allocated to 1 of 4 treatments (n = 15): supplemented with concentrate without oilseeds (CON), with whole cottonseed (CTS), rapeseed (RPS) or linseed (LNS). Oilseeds were supplemented during weeks 1-16 (spring period) and 17-22 (summer period), and the autumn period (wk 23-27) was used to evaluate treatment carryover effects. Cows fed CTS decreased CH4 yield by 14% compared to CON in spring, but these effects did not persist after 19 weeks of supplementation (summer). Compared to CON, RPS decreased milk yield and CTS increased milk fat concentration in both spring and summer. In summer, CTS also increased milk protein concentration but decreased milk yield, compared to CON. In spring, compared to CON, CTS decreased most milk medium-chain fatty acids (FA; 8:0, 12:0, 14:0 and 15:0) and increased stearic, linoleic and rumenic FA, and LNS increased CLA FA. There were no carry-over effects into the autumn period. In conclusion, supplementation of grazing dairy cows with whole oilseeds resulted in mild effects on methane emissions and animal performance. In particular, supplementing with CTS can decrease CH4 yield without affecting milk production, albeit with a mild and transient CH4 decrease effect. Long term studies conducted under grazing conditions are important to provide a comprehensive overview of how proposed nutritional CH4 mitigation strategies affect productivity, sustainability and consumer health aspects.

Feeding behavior of feedlot lambs fed diets with different energy and protein sources.

This study aimed to evaluate the effect of the replacement of ground corn by crude glycerin and soybean meal by cottonseed cake, in association or not, on feedlot lambs' feeding behavior. Thirty-two non-castrated crossbred ½ Dorper ½ Santa Inês lambs, with an average body weight of 18.52 ± 3.24 kg (mean ± standard deviation), were distributed in a completely randomized design, into four treatments and eight replicates per treatment, in a 2 × 2 factorial scheme. Four experimental diets were tested with different sources or combinations of energy and protein ingredients in the concentrate, respectively: (1) ground corn + soybean meal; (2) ground corn and cottonseed cake; (3) crude glycerin and soybean meal; and (4) crude glycerin and cottonseed cake. The glycerin decreased dry matter (DM) and neutral detergent fiber (NDF) (P < 0.001) intakes, whereas it increased times spent in feeding (P = 0.009; from 21.8 to 28.6), rumination (P = 0.006; from 56.2 to 67.2), and chewing (P < 0.001; from 79.4 to 97.2), in minutes/100 g of DM. Feeding efficiencies of DM (P = 0.003) and NDF (P = 0.008) were decreased by the diets. Protein sources increased (P = 0.018) the times spent in chewing and per period in rumination (P = 0.014). Cottonseed cake, as a protein source, up to a 112 g/kg DM level, can totally replace soybean meal in diets for feedlot lambs without effects on the intake, behavioral activities, and feeding efficiencies. In contrast, the crude glycerin energy source is not adequate to replace ground corn in lambs' diets.

Does the Effect of Replacing Cottonseed Meal with Dried Distiller's Grains on Nellore Bulls Finishing Phase Vary between Pasture and Feedlot?

The study aimed to evaluate the effect of replacing cottonseed meal by dried distiller's grains (DDG) in terms of efficiency in the productive aspects of beef cattle finishing in pasture versus feedlot. The experiment was conducted in a completely randomized design in a 2 × 3 factorial arrangement, with two production systems (pasture versus feedlot) and three supplements: CM, conventional supplement with cottonseed meal (CM) as a protein source; 50DDG: supplement with 50% replacement of CM by DDG; and 100DDG: 100% replacement. The effect of replacing CM by DDG on dry matter and nutrients intake and nutrients digestibility depends on the finishing system (p < 0.05). While in the pasture system animal consumed more nutrients in the CM, a greater intake was observed in the 100DDG in feedlot. The nutrients digestibility was lower in the pasture (p < 0.05). Animal performance and final body weight were higher in the feedlot (p < 0.0001), with averages of 1.57 kg/d and 566 kg of final body weight (FBW) for feedlot, and 0.99 kg/d and 504 kg FBW for pasture. The use of DDG does not change the animal performance finished in pasture or feedlot, and it is a viable alternative to replace conventional supplements in finishing phase in both systems in tropical environment.

Effects of the association between whole cottonseed and calcium salts of fatty acids on nutrient intake, feedlot performance, and carcass characteristics of Bos indicus animals offered a high-concentrate diet.

This experiment evaluated the effects of feeding whole cottonseed (WC) and/or calcium salts of fatty acids (CSFA) on dry matter intake (DMI), performance, and carcass characteristics of Bos indicus animals receiving a high-concentrate diet during the finishing phase. On day 0, 96 Nellore bulls were blocked according to initial shrunk body weight (BW; 302 ± 26.7 kg) into group pens (four animals/pen) and, within blocks, pens were randomly assigned to receive: 1) 15% of WC and 2% of CSFA (dry matter [DM] basis) of palm, cottonseed, and soybean oil (15WC; n = 6); 2) 10% of WC and 3% of CSFA (DM basis) of palm, cottonseed, and soybean oil (10WC; n = 6); 3) 5% of WC and 4% of CSFA (DM basis) of palm, cottonseed, and soybean oil (5WC; n = 6); and 4) 0% of WC and 5% of CSFA (DM basis) of palm, cottonseed, and soybean oil (0WC; n = 6). Diets were formulated to be isocaloric, isonitrogenous, and isolipidic. Experimental period lasted 108 d; DMI was evaluated daily, whereas blood samples and carcass measurements were obtained on days 0, 55, and 108 of the study. Upon slaughter on day 109, steaks were collected for determination of the chemical and fatty acid (FA) profile of the meat. No treatment effects (P ≥ 0.35) were observed on DMI, performance, average daily gain (ADG), carcass ultrasound measurements, and chemical variables of the steak. Nonetheless, including WC into the diets decreased C12:0, C16:0, C16:1 trans-9, C17:0, C18:0, C18:1 cis-9, C18:2 cis-9, cis-12, C18:3 cis-9, cis-12, cis-15, saturated, and unsaturated FA intake (P < 0.01). Moreover, adding WC increased DMI fluctuation and feed efficiency (FE; P = 0.03) but decreased marbling (P ≤ 0.03). A treatment × day interaction was observed (P < 0.01) for serum leptin concentration, as 10WC animals had greater leptin concentration on day 103 vs. other treatments (P < 0.01). Regarding steak FA profile, WC addition into the diet increased C18:2 cis-7, trans-9 and C18:3 cis-9, cis-12, cis-15 (P < 0.001), whereas saturated FA was quadratically affected (P = 0.02) and unsaturated FA was reduced for 15WC (P < 0.04). In summary, increasing levels of CSFA into isolipidic finishing diets containing WC did not negatively impact feedlot performance but reduced FE and increased marbling scores of B. indicus bulls, demonstrating its feasibility as a technology to improve carcass traits of low-marbling animals.

Protected and partially protected fat sources from ruminal degradation for pregnant sheep

Fat sources are important component on animal feed. However, ruminants did not evolve to efficient degrade lipids. Thus, it is necessary to study how its supplementation may affect animal performance. The objective of this study was to evaluate the use of different lipids sources over consumption, performance, metabolites, and feed behavior of pregnant sheep. The experiment was carried out at Capim Branco Experimental Farm, at goat and sheep sector of Federal University of Uberlândia, from August to November 2015. Twenty-four pregnant sheep were used, mean body weight 50 kg and approximate 12 months age. The animals were divided into three treatments (Control, palm oil protected fat and cottonseed). The corn silage was used as the bulk. The roughage: concentrate ratio was 60:40 (on dry matter). Measurements of body weight, body condition score and biometric measurements were taken every 21 days. Ingestive behavior evaluation was performed throughout the experiment. The consumption measurement was made in descriptive way. Shortly after birth, the sheep and their offspring were weighed, the barrel circumference was taken, and samples from the mother's blood were collected. The experimental design was completely randomized with repeated measurements in time. It was verified greater time spent with feeding and chewing in the treatment with cottonseed and consequently less time in idle. There was no statistical difference in weight, barrel circumference (BC) and body condition score (BCS) of the sheep under the different treatments. There was a linear growth in BC measurements and sheep weight over the evaluated periods. There were differences between the treatments for the offspring's average weight. The use of cottonseed as a fat source increases the birth weight of the progeny and improves pregnant sheep metabolic profile in relation to inert fat.

Protected and partially protected fat sources from ruminal degradation for pregnant sheep

Fat sources are important component on animal feed. However, ruminants did not evolve to efficient degrade lipids. Thus, it is necessary to study how its supplementation may affect animal performance. The objective of this study was to evaluate the use of different lipids sources over consumption, performance, metabolites, and feed behavior of pregnant sheep. The experiment was carried out at Capim Branco Experimental Farm, at goat and sheep sector of Federal University of Uberlândia, from August to November 2015. Twenty-four pregnant sheep were used, mean body weight 50 kg and approximate 12 months age. The animals were divided into three treatments (Control, palm oil protected fat and cottonseed). The corn silage was used as the bulk. The roughage: concentrate ratio was 60:40 (on dry matter). Measurements of body weight, body condition score and biometric measurements were taken every 21 days. Ingestive behavior evaluation was performed throughout the experiment. The consumption measurement was made in descriptive way. Shortly after birth, the sheep and their offspring were weighed, the barrel circumference was taken, and samples from the mother's blood were collected. The experimental design was completely randomized with repeated measurements in time. It was verified greater time spent with feeding and chewing in the treatment with cottonseed and consequently less time in idle. There was no statistical difference in weight, barrel circumference (BC) and body condition score (BCS) of the sheep under the different treatments. There was a linear growth in BC measurements and sheep weight over the evaluated periods. There were differences between the treatments for the offspring's average weight. The use of cottonseed as a fat source increases the birth weight of the progeny and improves pregnant sheep metabolic profile in relation to inert fat.(AU)

Effect of the diet on performance characteristics and quality of meat fat of lamb by principal component analysis

The objective of this study was to evaluate the effect of use of agroindustrial co-products in the diet of lambs on the performance and nutritional quality of meat fat. Twenty lambs were distributed into four experimental groups: control diet (C) or diets containing cottonseed (CS), sunflower meal (SFM), and castor cake (CC). During the experimental period, data on dry matter consumption (DMC) and total weight gain (TWG) were collected. After slaughter, samples of Logissimus dorsi (LD) muscle were taken for fatty acids and cholesterol analysis. Diets C and CS provided similar characteristics of fat quality and animal performance. However, SFM and CC diets tend to form isolated groups, with different fat and performance characteristics. The SFM diet results in meat with a higher saturated fatty acids and cholesterol content. The CC diet is related to improved performance characteristics and lower cholesterol content. In conclusion, the inclusion of CC in the experimental diets provided, in the proportions used in this experiment, better characteristics of animal performance and meat fat quality.(AU)

Effect of the diet on performance characteristics and quality of meat fat of lamb by principal component analysis

The objective of this study was to evaluate the effect of use of agroindustrial co-products in the diet of lambs on the performance and nutritional quality of meat fat. Twenty lambs were distributed into four experimental groups: control diet (C) or diets containing cottonseed (CS), sunflower meal (SFM), and castor cake (CC). During the experimental period, data on dry matter consumption (DMC) and total weight gain (TWG) were collected. After slaughter, samples of Logissimus dorsi (LD) muscle were taken for fatty acids and cholesterol analysis. Diets C and CS provided similar characteristics of fat quality and animal performance. However, SFM and CC diets tend to form isolated groups, with different fat and performance characteristics. The SFM diet results in meat with a higher saturated fatty acids and cholesterol content. The CC diet is related to improved performance characteristics and lower cholesterol content. In conclusion, the inclusion of CC in the experimental diets provided, in the proportions used in this experiment, better characteristics of animal performance and meat fat quality.

Supplementation with calcium salts of cottonseed oil improves performance of Bos indicus animals consuming finishing diets.

Lipid ingredients are often used into feedlot cattle diets, primarily to increase energy density and improve efficiency parameters of the herd. Therefore, this study was designed to evaluate the effects of including calcium salts of fatty acids (CSFA) and increasing levels of cottonseed byproducts into feedlot diets. On day 0 of the study, 96 Bos indicus bullocks were individually weighed twice and initial body weight (BW) was considered the average of both measurements (initial BW = 287 ± 22.4 kg). Bulls were ranked by initial BW, allocated into 1 of 12 feedlot pens (eight bulls per pen), and pens randomly assigned to one of three treatments: 1) inclusion of 15.0% [dry matter (DM) basis] of cottonseed byproducts into the finishing diet (CTS-15; n = 4), 2) inclusion of 22.0% (DM basis) of cottonseed byproducts into the finishing diet (CTS-22; n = 4), and 3) inclusion of 2.7% (DM basis) of CSFA of cottonseed oil into the finishing diet (CSFA; n = 4). The experimental period lasted 135 d and consisted of 5 d of preadaptation, 15 d of adaptation (ADP), 31 d of growing (GRO), and 84 d of finishing (FIN). Performance and carcass characteristics data were evaluated at the end of the experimental period. A treatment × period interaction was observed on total DM intake (DMI; P < 0.0001), given that no treatment differences were observed during ADP (P > 0.33), whereas CSFA-supplemented animals had a reduced DMI during GRO and FIN phases (P < 0.05). When individual mean nutrient intake was evaluated, CSFA supplementation caused a reduction in crude protein and physically effective neutral detergent fiber intake (P ≤ 0.05), and tended to reduce metabolizable energy, net energy for maintenance and gain intake (P = 0.06). Additionally, CSFA inclusion or CTS increase into the diet did not affect final BW, BW change, average daily gain (ADG), hot carcass weight, carcass ADG, and yield gain (P ≥ 0.11). On the other hand, CSFA reduced DMI as percentage of BW and improved feed efficiency (FE; P < 0.02) and also tended to improve biological conversion (BC; P = 0.07) versus CTS. Similarly, increasing CTS byproducts in the diet improved FE and BC (P = 0.02) but also tended to increase dressing percentage (DP; P = 0.08). In summary, including CSFA into feedlot diets reduced DMI but improved FE and BC of beef cattle, demonstrating the efficacy of this technology on feedlot beef cattle diets. Moreover, increasing cottonseed byproducts into the diets also benefited FE, BC, and DP of finishinw B. indicus cattle.

Efeito de diferentes concentrações de ingestão de gossipol livre sobre morfometria testicular e qualidade seminal de touros da raça Nelore / Effect of free gossypol intake in different levels on the testicular morphometry and seminal quality of Nelore bulls

A cotonicultura tem forte fator de impacto na economia nacional, e o estado de Mato Grosso se destaca por ser o maior produtor de algodão herbáceo e deter o maior rebanho bovino do país, condições essas que estimulam o uso do caroço, da torta e do farelo de algodão na alimentação animal. Considerando que o gossipol está presente nos subprodutos do algodão e que seus efeitos sobre a reprodução podem reduzir a fertilidade dos animais, objetivou-se, com essa pesquisa, avaliar o efeito da ingestão de dietas com diferentes teores de gossipol livre por dia sobre a morfometria testicular e a qualidade seminal de touros da raça Nelore. Foram utilizados 28 touros, distribuídos aleatoriamente em seis tratamentos: T0, 0 grama de gossipol livre/touro/dia ; T1, 1,08 grama de gossipol livre/touro/dia; T2, 2,07 gramas de gossipol livre/touro/dia; T3, 3,24 gramas de gossipol livre/touro/dia; T4, 3,82 gramas de gossipol livre/touro/dia e T5, 5,08 gramas de gossipol livre/touro/dia. Os animais de cada tratamento foram mantidos confinados em área média de 100m2, dotada de bebedouro, cochos para mistura mineral e para volumoso/concentrado. O consumo de 3,24 gramas de gossipol livre/touro/dia alterou a qualidade espermática e a morfometria testicular de touros.(AU)

The cotton industry has a strong impact factor in the Brazilian economy and the state of Mato Grosso stands out for being the largest upland-type cotton producer and also holds the largest cattle herd in the country, conditions that stimulate the use of cottonseed pie and cottonseed meal in animal feed. Whereas gossypol is present in cotton by-products and their effects on reproduction can reduce the animal fertility, this research evaluated the effect of diets with different free gossypol contents in the testicular morphometry and semen quality of Nelore bulls. 28 bulls were randomly distributed in six treatments: T0, receiving no free gossypol; T1, receiving 1.08g / bull / day; T2, receiving 2.07g / bull / day; T3, receiving 3.24g / bull / day; T4, receiving 3.82g / bull / day and T5, receiving 5.08g / bull / day of free gossypol respectively. The animals in each treatment were kept confined in an averaged area of 100m2, having fresh water, troughs for mineral mixture and roughage / concentrate. The consumption of 3.24g of free gossypol / bull / day altered the bulls sperm quality and testicular morphometry.(AU)

Efeito de diferentes concentrações de ingestão de gossipol livre sobre morfometria testicular e qualidade seminal de touros da raça Nelore / Effect of free gossypol intake in different levels on the testicular morphometry and seminal quality of Nelore bulls

A cotonicultura tem forte fator de impacto na economia nacional, e o estado de Mato Grosso se destaca por ser o maior produtor de algodão herbáceo e deter o maior rebanho bovino do país, condições essas que estimulam o uso do caroço, da torta e do farelo de algodão na alimentação animal. Considerando que o gossipol está presente nos subprodutos do algodão e que seus efeitos sobre a reprodução podem reduzir a fertilidade dos animais, objetivou-se, com essa pesquisa, avaliar o efeito da ingestão de dietas com diferentes teores de gossipol livre por dia sobre a morfometria testicular e a qualidade seminal de touros da raça Nelore. Foram utilizados 28 touros, distribuídos aleatoriamente em seis tratamentos: T0, 0 grama de gossipol livre/touro/dia ; T1, 1,08 grama de gossipol livre/touro/dia; T2, 2,07 gramas de gossipol livre/touro/dia; T3, 3,24 gramas de gossipol livre/touro/dia; T4, 3,82 gramas de gossipol livre/touro/dia e T5, 5,08 gramas de gossipol livre/touro/dia. Os animais de cada tratamento foram mantidos confinados em área média de 100m2, dotada de bebedouro, cochos para mistura mineral e para volumoso/concentrado. O consumo de 3,24 gramas de gossipol livre/touro/dia alterou a qualidade espermática e a morfometria testicular de touros.(AU)

The cotton industry has a strong impact factor in the Brazilian economy and the state of Mato Grosso stands out for being the largest upland-type cotton producer and also holds the largest cattle herd in the country, conditions that stimulate the use of cottonseed pie and cottonseed meal in animal feed. Whereas gossypol is present in cotton by-products and their effects on reproduction can reduce the animal fertility, this research evaluated the effect of diets with different free gossypol contents in the testicular morphometry and semen quality of Nelore bulls. 28 bulls were randomly distributed in six treatments: T0, receiving no free gossypol; T1, receiving 1.08g / bull / day; T2, receiving 2.07g / bull / day; T3, receiving 3.24g / bull / day; T4, receiving 3.82g / bull / day and T5, receiving 5.08g / bull / day of free gossypol respectively. The animals in each treatment were kept confined in an averaged area of 100m2, having fresh water, troughs for mineral mixture and roughage / concentrate. The consumption of 3.24g of free gossypol / bull / day altered the bulls sperm quality and testicular morphometry.(AU)