Caesium-137 in the muscles of game animals in 2015-2022 - levels and time trend.

Introduction: Radioactive caesium-137 occurring in the environment may be taken up by plants and animals and pose a trophic threat to humans. Game animals living in forest ecosystems are very good bioindicators of the level of environmental contamination by ionising radiation. The main species measurably exposed to caesium-137 are the wild boar (Sus scrofa), the roe deer (Capreolus capreolus), and the red deer (Cervus elaphus). The study determined the levels of Cs-137 in muscle samples of these game animals in 2015-2022. Material and Methods: Using gamma radiation spectrometry, 858 samples of game animal muscle tissue were examined: 508 wild boar, 145 roe deer and 205 red deer samples. Results: Concentrations of Cs-137 varied widely (from minimum detectable activity (MDA) values to over 4,000 Bq/kg). In 63.4% of cases, the obtained concentrations exceeded the MDA. The permissible limit (600 Bq/kg for food) was exceeded in nine wild boar muscle samples, whereas it was not even exceeded once in roe or red deer muscle. The average concentration in wild boar was three times higher than in roe and red deer and amounted to 42.84 Bq/kg. The highest concentration of Cs-137 in wild boar muscle was 4,195 ± 372.0 Bq/kg, in roe deer muscle it was 111.5 ± 12.50 Bq/kg, and in red deer muscle was 86.70 ± 3.470 Bq/kg. Conclusion: The seven years' data indicate that wild boar absorb the most caesium-137 among game animals. The concentrations of Cs-137 in the muscle of game animals in the years 2015-2022 were at a nearly constant level, a very slow diminution being noticeable over time in roe and red deer muscle.

Long-term behaviour of Cs-137, Cs-133 and K in beech trees of French forests.

In the long-term after atmospheric deposit onto a forest ecosystem, Cs-137 becomes incorporated into the biogeochemical cycle of stable elements and progressively reaches a quasi-equilibrium state. This study aimed at determining to what extent Cs-137 activity distribution in tree vegetation could be predicted from that of stable caesium (Cs-133) and potassium (K), which are known to be stable chemical analogues and competitors for Cs-137 intake in tree organs. Field campaigns that focused on beech trees (Fagus sylvatica L.) were conducted in 2021 in three French forest stands with contrasted characteristics regarding either the contribution of global vs. Chornobyl fallouts, soil or climatic conditions. Decades after Cs-137 fallouts, it was found that more than 80% of the total radioactive inventory in the system remained confined in the top 20 cm mineral layers, while organic layers and beech vegetation (including roots) contributed each to less than 1.5%. The enhanced downward migration of Cs-137 in cambisol than podzol forest sites was presumably due to migration of clay particles and bioturbation. The distribution of Cs-137 and Cs-133 inventories in beech trees was very similar among sites but differed from that of K due a higher accumulation of Cs isotopes in roots (40-50% vs. < 25% for K). The aggregated transfer factor (Tag) of Cs-137 calculated for aerial beech organs were all lower than those reported in literature more than 20 years ago, this suggesting a decrease of bioavailability in soil due to ageing processes. Regarding their variability, Tags were generally lower by a factor 5 at the cambisol site, which was fairly well explained by a much higher value of RIP (radiocesium immobilisation potential). Cs-137 concentrations in trees organs normalized by the soil exchangeable fractions were linearly correlated to those of Cs-133 and the best fit was found for the linear regression model without intercept indicating that no more contribution of the foliar uptake could be observed on long term. Provided that the vertical distribution of caesium concentrations and fine root density are properly measured or estimated, Cs-133 was shown to be a much better proxy than K to estimate the root transfer of Cs-137.

Calibration of medical gamma cameras for estimation of internal contamination from137Cs.

Calibration of 22 gamma camera units was performed at 15 hospitals in southern and western Sweden to estimate137Cs contamination in humans in a supine static geometry, with a new developed calibration protocol and phantom. The minimum detectable activities (MDAs) and the estimated committed effective doses (CEDs) were calculated for each calibration. Generic calibration factors were calculated for five predetermined groups based on the detector type and manufacturer. Group 1 and 2 included NaI-based gamma cameras from General Electrics (GEs) with a crystal thickness of 5/8'' and 3/8'' respectively. Group 3 and 4 included NaI-based gamma cameras from Siemens Healthineers with a crystal thickness of 3/8'', with a similar energy window as the GE NaI-based cameras and a dual window respectively. Group 5 included semiconductor-based gamma cameras from GE with a CdZnTe (CZT) detector. The generic calibration factors were 60.0 cps kBq-1, 52.3 cps kBq-1, 50.3 cps kBq-1, 53.2 cps kBq-1and 48.4 cps kBq-1for group 1, 2, 3, 4, and 5 respectively. The MDAs ranged between 169 and 1130 Bq for all groups, with measurement times of 1-10 min, corresponding to a CED of 4.77-77.6µSv. A dead time analysis was performed for group 1 and suggested a dead time of 3.17µs for137Cs measurements. The dead time analysis showed that a maximum count rate of 232 kcps could be measured in the calibration geometry, corresponding to a CED of 108-263 mSv. It has been shown that semiconductor-based gamma cameras with CZT detectors are feasible for estimating137Cs contamination. The generic calibration factors derived in this study can be used for gamma cameras of the same models in other hospitals, for measurements in the same measurement geometry. This will increase the measurement capability for estimating internal137Cs contamination in the recovery phase following radiological or nuclear events.

Ordinary-Portland-cement solidification of Cs-137 contaminated electric arc furnace dust from steel production industry in Thailand.

The cementation was used to immobilize the Cs-137 contaminated electric arc furnace dust (EAFD). Various mixing recipes were used to prepare the EAFD-cement waste form specimens. The strength test, the ANSI/ANS-16.1 leaching test and the immersion test were performed to judge whether the cured specimens satisfy the Radwaste disposal requirements. The strengths of all specimens were higher than the acceptable limit (3.45 MPa). The specimen's strength depended on the EAFD content, the water-to-binders ratio, and the curing time. Moreover, it could be affected by the leaching of the cement and EAFD components. The leaching results showed that the Cs-137 could be totally leached after ending of the test. There is a positive correlation between the quantity of Cs-137 in the leachate and the leachate pH. The Cs-137 leachability index (LI) decreased as the EAFD content and the ratio increased. The LI values ranged from 5.9 to 6.4. The Cs-137 leaching from the specimens could be controlled by the diffusion or the surface wash-off, depending on the recipes used. Additionally, the Cs-137 leaching might be controlled by multiple mechanisms. The findings reasonably recommend that the recipe with the ratio of 0.40 and 40 % EAFD could be used for the EAFD immobilizing.

Latent variable modeling of gamma-ray background in repeated measurements.

We propose a novel approach for background subtraction in repeated gamma-ray spectrometric measurements. This entirely data-driven method eliminates the need for Monte Carlo detector simulation. To accomplish this, we utilized the framework of Latent Variable Modeling, incorporating various matrix factorization techniques and artificial neural networks. Subsequently, we applied this method to estimate radionuclide activity through spectrum unmixing. Significant improvements in sensitivity, surpassing traditional methods, were observed for the test case scenario of aerosol filter measurements.

Effective half-lives for 137Cs in dairy milk from alpine ecosystems and the controlling factors.

Alpine regions in the federal state of Salzburg (Austria) have been intensively contaminated by Chernobyl fallout, necessitating long-term monitoring programs. The sites predominately affected are those in areas with soil developed on silicate bedrock, as these soils tend to be acidic, favouring high transfer factors for 137Cs. In addition, nutrient deficiency, low mineral and high organic matter content, and tough climatic conditions are causing the slow migration of 137Cs in the soil, which are associated with long effective half-lives in the biosphere. As a quantitative measure for effective half-lives, milk has been collected at nine alpine seasonal stock farming sites since 1988; at four sites, the monitoring is still ongoing (2020). For the period between 1999 and 2020, the decrease of 137Cs can be reasonably fitted with one effective half-life describing the time-trend. The effective half-lives obtained by this procedure vary between 9.3 ± 0.9 years and 18.8 ± 3.4 years. The effective half-lives show a weak negative correlation with the half-value depth of 137Cs, defined as the depth of the upper soil layer containing half of the deposited fallout inventory. The majority of the inventory is bound in the rooting zone of 0-10 cm, which is reflected by the small half value depths in the range between 3.2 and 4.4 cm. The soils investigated are acidic with pH values between 3.78 and 4.88, showing a pronounced negative correlation with the effective half-lives of 137Cs in milk. The data indicate that in these soils rich in organic matter, which are also almost totally devoid of clay minerals and have a very low clay size fraction, pH may be the dominating factor influencing the effective half-lives of 137Cs plant uptake and the subsequent contamination of milk.

Developments in Pb-210 methodologies to provide chronologies for environmental change.

Chronologies generated from core profiles to apply dates to environmental changes commonly use the measurement of the activity of radionuclides deposited and stratified with physical environmental material. The most commonly reported nuclide to define chronologies covering the last 150 years is Pb-210, for which accepted data processing methodologies in the literature have focussed on the constant rate of supply (CRS) model and the more recently published Bayesian Plum model. This short communication describes a validation approach using defined sediment layers referred to as 'varve' counting, which provide known points of reference to account for uncertainty between generated dates from each model using published Pb-210 measurements. A significant improvement in the chronologies was observed when applying reference date corrections to the models. This was shown to be essential in providing confidence in reported datasets and accuracy of predicted chronologies, which will better inform the interpretation of environmental change, e.g. sedimentation rates, climate change, pollution pathways and land degradation. Generated chronologies from both the CRS and Plum methods showed good agreement with the established varve dates (typically < 4-year difference).

Regularities of 137Cs distribution in the soil and vegetation cover of elementary landscape-geochemistry system within the forest test plot in the Chernobyl NPP exclusion zone.

The main goal of the investigation was to reveal the lateral and vertical regularities of element distribution in the elementary landscape-geochemical system (ELGS) type: summit-slope-closing depression. We used an isotope 137Cs as a tracer of migration of chemical elements in soil and vegetation cover. The study was performed in a test site characterizing undisturbed forest landscapes in the Russian zone of the Chernobyl accident. Investigated ELGS was 16-m long with a relative height difference of 1.5 m. Field measurements of 137Cs activity was performed with a 1-meter step by the modified portable gamma-spectrometer Violinist-III (USA). Cs-137 content in moss and soil cores sampled with the same lateral step was determined in the laboratory using Canberra (USA, HPGe detector). The upper soil layer 8-cm thick contained from 70 to 96% of 137Cs, and 89-99% of the total inventory was fixed in the top 20-cm layer. Cs-137 activity in both the soil and moss cover demonstrated a cyclic type of variability, which was described and modeled using Fourier analysis. Correlation between the actual and modeled activity values (r0.01 = 0.868) showed that three main harmonics are sufficient for representative modeling of the observed cycles. We infer that the revealed patterns are characteristic for most of the chemical elements and may be useful for practical purposes.

Analysis of 134Cs, 137Cs, and 60Co in aerosol samples: An intercomparison among laboratories / 中国辐射卫生

Objective To improve the analysis of 134Cs, 137Cs, and 60Co in aerosol samples by the national key radiation environment laboratories. Methods Intercomparison of analysis results of 134Cs, 137Cs, and 60Co in standard aerosol samples was performed among the national key radiation environment laboratories according to Gamma spectrometry method of analyzing radionuclides in biological samples (GB/T 16145-1995 ), and the intercomparison results were evaluated by the standard deviation. Results Six laboratories were involved in the intercomparison. For 134Cs, 50% of the laboratories showed a relative deviation less than 10%, and 50% showed a relative deviation of 10%-20%. For 137Cs, 33.3% of the laboratories showed a relative deviation less than 10%, and 76.7% showed a relative deviation of 10%-20%. For 60Cs, all laboratories showed a relative deviation less than 10%. The overall intercomparison results were acceptable. Conclusion The laboratories in this intercomparison show generally good results.

Gold Nanoparticles Enhancing Generation of ROS for Cs-137 Radiotherapy.

Radiotherapy is an important modality for the treatment of cancer, e.g., X-ray, Cs-137 γ-ray (peak energy: 662 keV). An important therapy pathway of radiation is to generate the double strand breaks of DNA to prohibit the proliferation of cancer cells. In addition, the excessive amount of reactive oxygen species (ROS) is induced to damage the organelles, which can cause cellular apoptosis or necrosis. Gold nanoparticles (GNPs) have been proven potential as a radiosensitizer due to the high biocompatibility, the low cytotoxicity and the high-Z property (Z = 79) of gold. The latter property may allow GNPs to induce more secondary electrons for generating ROS in cells as irradiated by high-energy photons. In this paper, the radiobiological effects on A431 cells with uptake of 55-nm GNPs were studied to investigate the GNPs-enhanced production of ROS on these cells as irradiated by Cs-137 γ-ray. The fluorescence-labeling image of laser scanning confocal microscopy (LSCM) shows the excessive expression of ROS in these GNPs-uptake cells after irradiation. And then, the follow-up disruption of cytoskeletons and dysfunction of mitochondria caused by the induced ROS are observed. From the curves of cell survival fraction versus the radiation dose, the radiosensitization enhancement factor of GNPs is 1.29 at a survival fraction of 30%. This demonstrates that the tumoricidal efficacy of Cs-137 radiation can be significantly raised by GNPs. Because of facilitating the production of excessive ROS to damage tumor cells, GNPs are proven to be a prospective radiosensitizer for radiotherapy, particularly for the treatment of certain radioresistant tumor cells. Through this pathway, the tumoricidal efficacy of radiotherapy can be raised.

Comparison and calibration of dose delivered by137Cs and x-ray irradiators in mice.

Objective.The Office of Radiological Security, U.S. Department of Energy's National Nuclear Security Administration, is implementing a radiological risk reduction program which seeks to minimize or eliminate the use of high activity radiological sources, including137Cs, by replacing them with non-radioisotopic technologies, such as x-ray irradiators. The main goal of this paper is to evaluate the equivalence of the dose delivered by gamma- and x-ray irradiators in mice using experimental measurements and Monte Carlo simulations. We also propose a novel biophantom as anin situdose calibration method.Approach.We irradiated mouse carcasses and 3D-printed mouse biophantoms in a137Cs irradiator (Mark I-68) and an x-ray irradiator (X-Rad320) at three voltages (160 kVp, 225 kVp and 320 kVp) and measured the delivered radiation dose. A Geant4-based Monte Carlo model was developed and validated to provide a comprehensive picture of gamma- and x-ray irradiation in mice.Main Results.Our Monte Carlo model predicts a uniform dose delivered in soft-tissue for all the explored irradiation programs and in agreement with the absolute dose measurements. Our Monte Carlo model shows an energy-dependent difference between dose in bone and in soft tissue that decreases as photon energy increases. Dose rate depends on irradiator and photon energy. We observed a deviation of the measured dose from the target value of up to -9% for the Mark I-68, and up to 35% for the X-Rad320. The dose measured in the 3D-printed phantoms are equivalent to that in the carcasses within 6% uncertainty.Significance.Our results suggest that 320 kVp irradiation is a good candidate to substitute137Cs irradiation barring a few caveats. There is a significant difference between measured and targeted doses for x-ray irradiation that suggests a strong need forin situcalibration, which can be achieved with 3D-printed mouse biophantoms. A dose correction is necessary for bone doses, which can be provided by a Monte Carlo calculation. Finally, the biological implications of the differences in dose rates and dose per photon for the different irradiation methods should be carefully assessed for each small-animal irradiation experiment.

Impact of WO3-Nanoparticles on Silicone Rubber for Radiation Protection Efficiency.

Silicone rubbers are a good choice for shielding materials because of having elastic and attenuating properties as well as cost-effectiveness. Thus, the aim of this study was to prepare ground-breaking silicone rubber samples by adding WO3-nanoparticles and testing the performance of their radiation shielding ability against Cs-137, Co-60, and Am-241 gamma energy. Increasing the concentration of WO3 nanoparticles in silicone rubber (SR) led to decreasing the half-value layer (HVL) and mean free path (MFP) values determined for the samples tested. Furthermore, the values of MFP and HVL upsurged according to the enhancement of the photon energy. It is noteworthy that the prepared silicone rubber (SR) systems with 50 and 60 wt% concentrations of WO3-nanoparticles displayed lower HVL than the Bi2O3-containing silicone rubber (SR) systems. In the same way, studied silicone rubber SR-W60 represented the lowest HVL comprising iron ore containing silicone rubber.

The Kuroshio radiocesium stream.

The Fukushima accident released short-lived Cs-134 and longer-lived Cs-137 to the ocean. The amount, although substantial, is much less than that produced during the atomic bomb tests 60 years ago. But, the latter has received little attention. Here we found only Cs-137 in waters near the origin of the Kuroshio. The speed of the Kuroshio current generally decreases with water depth, yet, the Cs-137 activity increases with depth to reach a subsurface maximum of 2.4 Bq m-3. As a result, a core of high Cs-137 flux, or a radiocesium stream, exists at approximately 200-400 m in depth. In total, the Kuroshio transports about 1 PBq year-1 Cs-137 northward between 121 and 123°E, 1000 times more than the 0.73-1.0 TBq year-1 discharged to the ocean at Fukushima in 2016-2018.

Multifractal features of activity concentration and stochastic risk assessment of naturally occurring and technogenic radionuclides in the soil of Yerevan, Armenia.

Spatial patterns and background ranges of naturally occurring radionuclides (NORs) (i.e. U-238, Th-232, K-40) and Cs-137 were studied in the urban soils of Yerevan, the capital city of Armenia. Multifractal Inverse Distance Weighting (MIDW) was used to generate and analyze distribution patterns of radionuclide activities. Based on Fourier transformation of radioactivity data, a spectral analysis was also applied to separate, where possible, background/baseline patterns from local anomalies: two ranges of background values were found to characterise the Yerevan territory. Specifically, in the south and south-east of Yerevan, the lower background ranges of U-238, Th-232 and K-40 comprised in the intervals 2.60-36.42 Bq/kg, 4.04-30.63 Bq/kg and 147.7-396.7 Bq/kg, respectively, were observed in association with the presence of sedimentary formations. In contrast, the higher ones were found, instead, in the central and northern parts of the city where andesite-basalt lavas and ignimbrite tuffs occur. Here, the background values rise to 142.4 Bq/kg, 138.76 Bq/kg and 1502 Bq/kg, respectively. As for the distribution of artificial Cs-137, its baseline levels in Yerevan seem to depend mostly on the global radioactive fallout and some local technogenic sources. Its distribution patterns partially differ from those of NORs. In the framework of this paper, Radium equivalent activity (RaEq), outdoor absorbed dose rate in air (ODRA) and annual effective dose equivalent (AEDEs) were also determined and mapped. They show a good coincidence of their spatial variations with those of NORs. The Monte Carlo simulation was used to assess excess lifetime cancer risk from a stochastic perspective. The related sensitivity analysis revealed that, among NORs, U-238 and Th-232 give the greatest contribution to the total variance (45.7% 42.8%, respectively). In comparison, K-40 has the lowest share (11.3%). Regarding Cs-137, a highly negligible contribution to the onset of health risks (accounting for 0.02%) was observed.

Microplastic inventory in sediment profile: A case study of Golden Horn Estuary, Sea of Marmara.

Assessment of microplastics (MPs) in sediment cores is necessary to unveil global plastic pollution since most of the plastic litter might have been stored in sediment columns. In the current study, MPs inventory was determined in a 105 cm sediment core, collected in the Golden Horn Estuary, Sea of Marmara. Radiodating of sediment profile by using naturally occurring 210Pb and fission product 137Cs allowed us to couple the retrospective of global MP production to sediment MPs inventory. More than 90% of total MPs inventory was found in the deep layer of the sediment column (below 15 cm). Small MPs (20-200 µm) were more abundant than large ones (200-4000 µm). Elevated concentrations of MPs were attributed to industrial and municipal effluent of Istanbul metropolitan. On a local scale, this study suggests that the Golden Horn Estuary was polluted with MPs before the 1950s, and the abundance of MPs reached a maximum in the 1980s. We also propose on a global scale that "the missing" plastics might have been buried in deep sediment and radiodating of sediment is useful to reveal their historical input records.

The assessment of the April 2020 chernobyl wildfires and their impact on Cs-137 levels in Belgium and The Netherlands.

In April 2020, several wildfires took place in and around the Chernobyl exclusion zone. These fires reintroduced radioactive particles deposited during the 1986 Chernobyl disaster into the atmosphere, causing concern about a possible radiation hazard. Several countries and several stations of the International Monitoring System measured increased Cs137 levels. This study presents the analyses made by RIVM and SCK CEN/RMI during the April 2020 wildfires. Furthermore, more in-depth research was performed after the wildfires. A statistical analysis of Cs137 detections is presented, comparing the April 2020 detections with historical detections. Inverse atmospheric transport modelling is applied to infer the total released Cs137 during the wildfires. Finally, it is assessed whether the Cs137 detections in Belgium and the Netherlands can be attributed to the wildfires.

Modeling Cell Survival Fraction and Other Dose-Response Relationships for Immunodeficient C.B-17 SCID Mice Exposed to 320-kV X Rays.

US homeland security concerns related to potential misuse of γ-ray-emitting radiation sources employed in radiobiological research (eg, shielded cesium-137 irradiators) led to recommendations by the National Research Council to conduct studies into possibly replacing γ-ray irradiators used in research involving small rodent and other models with X-ray instruments. A limiting factor is suitability of the X-ray photon energy spectra. The objective of our research was to demonstrate the suitability of the radiation energy spectrum of 320-kV X rays after filtration (HVL = 4 mm Cu) for in-vivo cytotoxicity studies in immunodeficient C.B-17 SCID mice. By using a previously-published Hazard Function (HF) model to characterize dose-response relationships for in vivo bone marrow and spleen cell survival fractions and also to characterize the acute lethality risk (hematopoietic syndrome mode) we demonstrate that the filtered 320-kV X-ray beam appears suitable for such studies. A key finding for C.B-17 SCID mice when compared to results previously obtained for immunocompetent C.B-17 mice is that the immunodeficient mice appear to be more radioresistant, implicating a possible role of the immune system capacity in radiosensitivity of mammals.

Wildfires in the Chornobyl exclusion zone-Risks and consequences.

Following the 1986 Chornobyl accident, an area approaching 5000 km2 surrounding the nuclear plant was abandoned, creating the Chornobyl exclusion zone (CEZ). Although this area likely contains the most radioactive terrestrial ecosystem on earth, the absence of humans and associated activities for nearly 35 years since the accident has resulted in increases in wildlife numbers. Both the Belarussian and Ukrainian components of the CEZ are now designated as nature reserves; together they form one of Europe's largest protected areas and have been described as an iconic example of rewilding. Forests and former agricultural land (now scrub) dominate the CEZ and wildfires are an annual event. In April 2020, the CEZ suffered its most widespread fires to date when greater than 800 km2 of the 2600 km2 Ukrainian portion of the CEZ was burnt. Large-scale fires in the CEZ have implications for wildlife, as they do elsewhere, but they also pose additional radioecological and radiological protection questions. We discuss the implications of wildfires in the CEZ, considering effects on wildlife and changes in radionuclide mobility. We also demonstrate that the risk to firefighters and the wider public from the inhalation of radionuclides in smoke resulting from fires in the CEZ is likely to be low. However, further experimental and modeling work to evaluate potential doses to firefighters from inhaled radioactive particles would be valuable, not least for reassurance purposes. Integr Environ Assess Manag 2021;17:1141-1150 © 2021 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).

Phytoremediation of 137Cs, 60Co, 241Am, and 239Pu from aquatic solutions using Chlamydomonas reinhardtii, Scenedesmus obliquus, and Chlorella vulgaris.

NOVELTY STATEMENT: Ecologically suitable methods for the decontamination of liquid radioactive waste or radioactively contaminated areas are becoming more and more important due to the pollution of the planet. We believe that phytoremediation of radionuclides using microalgae is one of the optimal ecological methods to decontamination of radioactive waste. Microalgae as unicellular organisms have a number of advantages over the other organisms used in bioremediation-high level of tolerance to the environment, fast growth rates, high tolerance to various pH levels, etc. In this study, we used 3 different strains of microalgae for phytoremediation of various radionuclides (137Cs, 60Co, 241Am, and 239Pu). This research was focused on ex situ phytoremediation of radionuclides using microalgae at various pH levels of radioactively contaminated solutions. Due to the ability of microalgae to adapt to sometimes even extreme pH values, this research may be interesting for many institutions and researchers dealing with more environmentally friendly methods of decontamination of radioactive waste.