Influence of High Strain Dynamic Loading on HEMA-DMAEMA Hydrogel Storage Modulus and Time Dependence.

Hydrogels have been extensively studied for biomedical applications such as drug delivery, tissue-engineered scaffolds, and biosensors. There is a gap in the literature pertaining to the mechanical properties of hydrogel materials subjected to high-strain dynamic-loading conditions even though empirical data of this type are needed to advance the design of innovative biomedical designs and inform numerical models. For this work, HEMA-DMAEMA hydrogels are fabricated using a photopolymerization approach. Hydrogels are subjected to high-compression oscillatory dynamic mechanical loading at strain rates equal to 50%, 60%, and 70%, and storage and loss moduli are observed over time, e.g., 72 h and 5, 10, and 15 days. As expected, the increased strains resulted in lower storage and loss moduli, which could be attributed to a breakdown in the hydrogel network attributed to several mechanisms, e.g., increased network disruption, chain scission or slippage, and partial plastic deformation. This study helps to advance our understanding of hydrogels subjected to high strain rates to understand their viscoelastic behavior, i.e., strain rate sensitivity, energy dissipation mechanisms, and deformation kinetics, which are needed for the accurate modeling and prediction of hydrogel behavior in real-world applications.

Iron-Catalyzed Sulfonylmethylation of Imidazo[1,2-α]pyridines with N,N-Dimethylacetamide and Sodium Sulfinates.

Functionalized imidazo[1,2-α]pyridines are important scaffolds in pharmaceuticals. Herein, we present an efficient 3-sulfonylmethylation protocol for imidazo[1,2-α]pyridines by sodium sulfinates in DMA and H2O (2:1) via an FeCl3-catalyzed three-component coupling reaction. Various sulfonylmethyl imidazo[1,2-α]pyridines were thus afforded in high yields with excellent functional group tolerance. A plausible oxidation-addition mechanism was proposed.

Toward the Manufacturing of a Non-Toxic High-Performance Biobased Epoxy-Hemp Fibre Composite.

This study describes the production of a new biobased epoxy thermoset and its use with long hemp fibres to produce high-performance composites that are totally biobased. The synthesis of BioIgenox, an epoxy resin derived from a lignin biorefinery, and its curing process have been optimised to decrease their environmental impact. The main objective of this study is to characterise the rheology and kinetics of the epoxy system with a view to optimising the composite manufacturing process. Thus, the epoxy resin/hardener system was chosen considering the constraints imposed by the implementation of composites reinforced with plant fibres. The viscosity of the chosen mixture shows the compatibility of the formulation with the traditional implementation processes of the composites. In addition, unlike BPA-a precursor of diglycidyl ether of bisphenol A (DGEBA) epoxy resin-BioIgenox and its precursor do not have endocrine disrupting activities. The neat polymer and its unidirectional hemp fibre composite are characterised using three-point bending tests. Results measured for the fully biobased epoxy polymer show a bending modulus, a bending strength, a maximum strain at failure and a Tg of, respectively, 3.1 GPa, 55 MPa, 1.82% and 120 °C. These values are slightly weaker than those of the DGEBA-based epoxy material. It was also observed that the incorporation of fibres into the fully biobased epoxy system induces a decrease in the damping peak and a shift towards higher temperatures. These results point out the effective stress transfers between the hemp fibres and the fully biobased epoxy system. The high mechanical properties and softening temperature measured in this work with a fully biobased epoxy system make this type of composite a very promising sustainable material for transport and lightweight engineering applications.

Effect of Nanoceria Suspension Addition on the Physicochemical and Mechanical Properties of Hybrid Organic-Inorganic Hydroxyapatite Composite Scaffolds.

In the current study, the synthesis of hydroxyapatite-ceria (HAP-CeO2) scaffolds is attempted through a bioinspired chemical approach. The utilized colloidal CeO2 suspension presents antifungal activity against the Aspergillus flavus and Aspergillus fumigatus species at concentrations higher than 86.1 ppm. Three different series of the composite HAP-CeO2 suspensions are produced, which are differentiated based on the precursor suspension to which the CeO2 suspension is added and by whether this addition takes place before or after the formation of the hydroxyapatite phase. Each of the series consists of three suspensions, in which the pure ceria weight reaches 4, 5, and 10% (by mass) of the produced hydroxyapatite, respectively. The characterization showed that the 2S series's specimens present the greater alteration towards their viscoelastic properties. Furthermore, the 2S series's sample with 4% CeO2 presents the best mechanical response. This is due to the growth of needle-like HAP crystals during lyophilization, which-when oriented perpendicular to the direction of stress application-enhance the resistance of the sample to deformation. The 2S series's scaffolds had an average pore size equal to 100 µm and minimum open porosity 89.5% while simultaneously presented the lowest dissolution rate in phosphate buffered saline.

Risk assessment of small organoarsenic species in food.

The European Commission asked EFSA for a risk assessment on small organoarsenic species in food. For monomethylarsonic acid MMA(V), decreased body weight resulting from diarrhoea in rats was identified as the critical endpoint and a BMDL10 of 18.2 mg MMA(V)/kg body weight (bw) per day (equivalent to 9.7 mg As/kg bw per day) was calculated as a reference point (RP). For dimethylarsinic acid DMA(V), increased incidence in urinary bladder tumours in rats was identified as the critical endpoint. A BMDL10 of 1.1 mg DMA(V)/kg bw per day (equivalent to 0.6 mg As/kg bw per day) was calculated as an RP. For other small organoarsenic species, the toxicological data are insufficient to identify critical effects and RPs, and they could not be included in the risk assessment. For both MMA(V) and DMA(V), the toxicological database is incomplete and a margin of exposure (MOE) approach was applied for risk characterisation. The highest chronic dietary exposure to DMA(V) was estimated in 'Toddlers', with rice and fish meat as the main contributors across population groups. For MMA(V), the highest chronic dietary exposures were estimated for high consumers of fish meat and processed/preserved fish in 'Infants' and 'Elderly' age class, respectively. For MMA(V), an MOE of ≥ 500 was identified not to raise a health concern. For MMA(V), all MOEs were well above 500 for average and high consumers and thus do not raise a health concern. For DMA(V), an MOE of 10,000 was identified as of low health concern as it is genotoxic and carcinogenic, although the mechanisms of genotoxicity and its role in carcinogenicity of DMA(V) are not fully elucidated. For DMA(V), MOEs were below 10,000 in many cases across dietary surveys and age groups, in particular for some 95th percentile exposures. The Panel considers that this would raise a health concern.

Performance Evaluation of Out-of-Spec Carbon Prepregs for Upcycling Purposes.

In view of exploring the possibility of upcycling aerospace scrap, cure characteristics of out-of-spec carbon fiber prepregs are investigated in this study. The cure behavior of the prepreg is examined in the form of the mechanical cure conversion state of the material using a Dynamic Mechanical Analyzer (DMA). Cure kinetics is modeled by comparing the storage modulus at the start of the reaction (E'0) and instantaneously (E't) during isothermal experiments with those of the fully cured material (E'∞) obtained from dynamic scans. The glass transition temperature Tg and the extent of reaction before gelation are modeled using the DiBenedetto model, where the Tg of each laminate is determined in a DMA, per standard ASTM D7028. The mechanical properties, the extent of cure, and the glass transition temperature of the cured laminates were determined according to industry and international standards. The maximum conversion at temperatures between 100 °C and 140 °C is approximately 80% (±5%). The modeled rate of conversion shows a reasonable match with the experimental data, exhibiting a maximum reaction rate at about 30-40% of the cure conversion. The predicted evolution of the Tg as a function of cure conversion using the DiBenedetto model provides a 94% match with the experimental data. The multi-stage cure cycle based on the models offers shorter cycle times and high-quality laminates. The mechanical test results indicate approximately a 13% and 15% decrease in tensile strength and modulus, respectively, compared to pristine ones. The experimental extent of cure of the cured laminates (95.4%) is in close agreement with that predicted by the model (97%). The porosity in the laminates is estimated to be approximately 2.4%, which is acceptable in several industries.

Optimizing Epoxy Molding Compound Processing: A Multi-Sensor Approach to Enhance Material Characterization and Process Reliability.

The in-line control of curing during the molding process significantly improves product quality and ensures the reliability of packaging materials with the required thermo-mechanical and adhesion properties. The choice of the morphological and thermo-mechanical properties of the molded material, and the accuracy of their determination through carefully selected thermo-analytical methods, play a crucial role in the qualitative prediction of trends in packaging product properties as process parameters are varied. This work aimed to verify the quality of the models and their validation using a highly filled molding resin with an identical chemical composition but 10 wt% difference in silica particles (SPs). Morphological and mechanical material properties were determined by dielectric analysis (DEA), differential scanning calorimetry (DSC), warpage analysis and dynamic mechanical analysis (DMA). The effects of temperature and injection speed on the morphological properties were analyzed through the design of experiments (DoE) and illustrated by response surface plots. A comprehensive approach to monitor the evolution of ionic viscosity (IV), residual enthalpy (dHrest), glass transition temperature (Tg), and storage modulus (E) as a function of the transfer-mold process parameters and post-mold-cure (PMC) conditions of the material was established. The reliability of Tg estimation was tested using two methods: warpage analysis and DMA. The noticeable deterioration in the quality of the analytical signal for highly filled materials at high cure rates is discussed. Controlling the temperature by increasing the injection speed leads to the formation of a polymer network with a lower Tg and an increased storage modulus, indicating a lower density and a more heterogeneous structure due to the high heating rate and shear heating effect.

Structural, radiation shielding, thermal and dynamic mechanical analysis for waste rubber/EPDM rubber composite loaded with Fe2O3 for green environment.

Increasing waste rubber recycling produces a specious range of products for many valuable applications. Waste Rubber/EPDM composite with different concentrations was prepared. Infrared spectroscopy (FTIR) is used to identify the chemical composition. A water absorption test, Dynamic mechanical analysis (DMA), and Thermal Gravimetric Analysis (TGA) were performed. The (75/25) WR/EPDM rubber composite exhibited the best behavior with the highest mechanical performance. Fe2O3 was added to (75/25) WR/EPDM rubber composite. Water absorption, FTIR, TGA, and DMA were investigated. The composite performance was improved with increasing Fe2O3 content. The linear attenuation coefficients (µ) were also measured as a function of the concentrations of Fe2O3 for γ-ray energy 662 keV by using 137Cs point source; the radiation shielding can be denoted by numbers of parameters like mass attenuation coefficient (µm), half value layer (HVL), Tenth value layer TVL and radiation protection efficiency (RPE%), radiation protection efficiency increased as Fe2O3 increased.

Baseline study for the total mercury determination in Yemeni fish.

The high levels of mercury toxicity in humans make it necessary to monitor mercury levels in food, pharmaceuticals, and the environment to minimize human exposure. Between June 2020 and October 2021, researchers collected 240 fish samples from different locations along the Yemeni coast to evaluate mercury contamination. The Direct Mercury Analyzer was used to determine the concentration of mercury in each sample. To ensure method accuracy, a series of triplicate mercury concentration analyses were conducted. The samples ranged from 2 to 100 ng to determine linearity and repeatability i.e., within-day variation. The results showed a high level of precision, with a correlation coefficient of 0.9990 and a repeatability of 1.34 %-5.62 % RSD range. The method was also highly accurate, as the mercury recovery results from the contaminated fish samples ranged from 96.77 % to 105.14 %. The limits of detection and quantitation of mercury were 0.0015 ppm and 0.0049 ppm, respectively. This allowed the method to detect trace amounts of mercury in fish meat. Mercury concentration in the 240 fish samples did not exceed the FDA, but below the 0.5 ppm specified limit of YSMO.

Mechanical, Thermal, and Physicochemical Properties of Filaments of Poly (Lactic Acid), Polyhydroxyalkanoates and Their Blend for Additive Manufacturing.

Polymeric blends are employed in the production of filaments for additive manufacturing to balance mechanical and processability properties. The mechanical and thermal properties of polymeric filaments made of poly (lactic acid) (PLA), polyhydroxyalkanoates (PHA), and its blend (PLA-PHA) are investigated herein and correlated to their measured structural and physicochemical properties. PLA exhibits the highest stiffness and tensile strength, but lower toughness. The mechanical properties of the PLA-PHA blend were similar to those of PLA, but with a significantly higher toughness. Despite the lower mechanical properties of neat PHA, incorporating a small amount (12 wt.%) of PHA into PLA significantly enhances toughness (approximately 50%) compared to pure PLA. The synergistic effect is attributed to the spherulitic morphology of blended PHA in PLA, promoting interactions between the amorphous regions of both polymers. Thermal stability is notably improved in the PLA-PHA blend, as determined by thermogravimetric analysis. The blend also exhibits lower cold crystallization and glass transition temperatures as compared to PLA, which is beneficial for additive manufacturing. Following additive manufacturing, X-ray photoelectron spectroscopic showed that the three filaments present an increase in C-C and C=O bonds associated with the loss of C-O bonds. The thermal process induces a slight increase in crystallinity in PHA due to chain reorganization. The study provides insights into the thermal and structural changes occurring during the melting process of additive manufacturing.

Dimethylformamide dimethyl acetal reagent for in situ chiral analyses of organic molecules on Titan with the Dragonfly mass spectrometer space instrument (Dragonfly mission).

Thanks to the Cassini-Huygens space mission between 2004 and 2017, a lot was learned about Titan, the biggest satellite of Saturn, and its intriguing atmosphere, surface, and organic chemistry complexity. However, key questions about the potential for the atmosphere and surface chemistry to produce organic molecules of direct interest for prebiotic chemistry and life did not find an answer. Due to Titan potential as a habitable world, NASA selected the Dragonfly space mission to be launched in 2027 to Titan's surface and explore the Shangri-La surface region for minimum 3 years. One of the main goals of this mission will be to understand the past and actual abundant prebiotic chemistry on Titan, especially using the Dragonfly Mass Spectrometer (DraMS). Two recently used sample pre-treatments for Gas Chromatography - Mass Spectrometry (GC-MS mode of DraMS) analyses are planned prior analysis to extract refractory organic molecules of interest for prebiotic chemistry and astrobiology. The dimethylformamide dimethylacetal (DMF-DMA) derivatization reaction offers undoubtedly an opportunity to detect biosignatures by volatilizing refractory biological or prebiotic molecules and conserving the chiral carbons' conformation while an enantiomeric excess indicates a chemical feature induced primarily by life (and may be aided on the primitive systems by light polarization). The goal of this study is to investigate the ageing of DMF-DMA in DraMS (and likely MOMA) capsules prior to in situ analysis on Titan (or Mars). The main results highlighted by our work on DMF-DMA are first its satisfactory stability for space requirements through time (no significant degradation over a year of storage and less than 30 % of lost under thermal stress) to a wide range of temperature (0 °C to 250 °C), or the presence of water and oxidants during the derivatization reaction (between 0 and 10 % of DMF-DMA degradation). Moreover, this reagent derivatized very well amines and carboxylic acids in high or trace amounts (ppt to hundreds of ppm), conserving their molecular conformation during the heat at 145 °C for 3 min (0 to 4% in the enantiomeric form change).

Effect of Irradiation Temperature and Atmosphere on Aging of Epoxy Resins for Superconducting Magnets.

The superconducting magnets of future particle accelerators will be exposed to high irradiation doses at cryogenic temperatures. To investigate the effect of irradiation temperature and atmosphere on the aging behavior, we have characterized the changes in thermomechanical properties of six epoxy resins for potential use in superconducting magnets after irradiation up to 20 MGy in ambient air, inert gas, and liquid helium. Based on the results obtained by Dynamic Mechanical Analysis (DMA), we discuss the effect of irradiation temperature and the presence of oxygen. The irradiation temperature can have a strong influence on the rates at which cross-linking and chain scission occur.

[The study on biological exposure index of occupational exposure to arsenic and its inorganic compounds].

Objective: To establish biological exposure index (BEI) of occupational exposure to arsenic and its inorganic compounds through occupational epidemiology and the regression analysis of internal and external exposure of workers. Methods: In November 2021, 125 workers with occupational exposure to arsenic and its inorganic compounds and 49 office administrators in a non-ferrous metal smelter in Yunnan Province were selected as the exposure group and control group, respectively. Air samples from the workplace of the study subjects on weekdays were collected and arsenic concentrations were determined. Urine samples were collected in end-of-work weekend and high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) was used to detect the levels of trivalent inorganic arsenic (iAs(3+)) , pentavalent inorganic arsenic (iAs(5+)) , monomethyl arsenic (MMA) and dimethyl arsenic (DMA) in urine. The correlations between arsenic concentration in the workplace air and arsenic species in urine of workers were analyzed. Arsenic exposure concentration and the level of urinary arsenic (ΣiAs+MMA+DMA) of workers was analyzed by linear regression and the BEI of arsenic and its inorganic compounds in the workplace was proposed based on the results of micronucleus test. Results: The median of time-weighted average concentration (C(TWA)) of arsenic in the workplace air of the exposure group was 0.0116 mg/m(3), and the over-standard rate was 71.2% (89/125) . The concentrations of iAs(3+), iAs(5+), inorganic arsenic (iAs=ΣiAs(3+)+iAs(5+)) 、MMA、DMA and urinary arsenic in the exposure group were higher than those in the control group at the end of shift, and the differences were statistically significant (P<0.05) . The concentration of arsenic in the workplace air had the strongest correlation with the concentration of urinary arsenic at the end of the shift (r(s)=0.909, P<0.001) . The regression equation was lg (y) =7.662+2.968lg (x) (r=0.821, P<0.05) . According to the occupational exposure limit (OEL) of arsenic in China, the concentration of urinary arsenic in the end-of-work weekend was calculated to be 53.2 µg/L. Combined with the results of micronucleus test, the BEI of occupational exposure to arsenic and its inorganic compounds in the workplace was proposed to be 50 µg/L. Conclusion: The urinary arsenic in the end-of-work weekend can be used as a biomarker of occupational exposure to arsenic, and its BEI is recommended to be 50 µg/L.

Characterization of size-resolved effective density of atmospheric particles in an urban atmosphere in Southern China.

Effective density (ρeff) is one of the most important physical properties of atmospheric particles, providing important references in exploring the emissions and aging processes of fresh particles. In this study, a combined system of differential mobility analyzer, centrifugal particle mass analyzer, and condensation particle counter was used to periodically measure the ρeff of atmospheric particles in Shenzhen from Oct. 2021 to Jan. 2022. Results showed that the ρeff of particles with various size presented a bimodal distribution, which could be divided into main density (ρm, main peak, corresponding to relatively dense particles after aging) and sub density (ρs, sub peak, corresponding to fresh particles). The occurrence frequencies of ρs of particles with diameters of 50 and 80 nm were less than 20%, but were as high as about 40% of that with diameters from 120 to 350 nm. The ρm showed increasing trend with the size of particles, while ρs decreased as the increasing of the size of particles. The ρeff on pollution day varied significantly with chemical compositions. The increasing of the proportion of sulfate could promote the increasing of ρeff, while black carbon and organic matter caused opposite effects, which may be related to various factors, including the difference of the material density and morphology of various chemical components. The ρeff of 50, 80 and 120 nm particles decreased considerably during the new particle formation event, indicating that organic condensation was an important contributor to new particle growth.

Comparative analysis between mercury levels in fish tissues evaluated using direct mercury analyzer and inductively plasma-coupled mass spectrometer.

Recent ecotoxicological studies have indicated mercury (Hg) contamination in aquatic ecosystems in the Amazon Basin. Although Hg contamination can be associated with small-scale gold mining, the soils of the Amazon region have naturally high Hg concentrations, and can be transported to aquatic ecosystems via deforestation and mining activities. Biomagnification of Hg can pose risks to the local human population; therefore, its concentration in fish tissues must be monitored consistently. Fast and sensitive Hg determination is required for continuously monitoring ecosystems impacted by mineral exploration. The direct mercury analyzer (DMA-80) is widely used for determining total Hg levels in tissue samples; it is fast and cost-effective, without requiring sample preparation. Here, we determined the sensitivity and specificity of Hg detection accomplished using DMA-80, and whether these results are reliable compared to those obtained using Inductively Coupled Plasma Mass Spectrometer (ICP-MS), which is the gold standard. We obtained 106 paired dried samples of muscle tissue from fish species occupying different trophic levels in the Lower Amazon region, and analyzed them using both equipment (DMA-80 and ICP-MS). The results obtained using DMA-80 had an overall Hg mean of 1.90 ± 0.18 mg/kg which was higher (p < 0.05) than the mean of those obtained using ICP-MS (1.55 ± 0.13 mg/kg). Linear regression analysis comparing the Hg levels obtained using both devices was within the 95% prediction interval, and a high coefficient of correlation showed agreement between the devices (r = 0.979; 0.069 to 0.986, 95% CI). Bland-Altman analysis showed that DMA-80 had a positive bias of 6.5% in relation to ICP-MS, which is more evident in samples with high Hg concentrations. DMA-80 was efficient in determining whether the Hg levels exceeded the maximum allowed levels required by the European Union, USA, and Brazil, showing a specificity and sensitivity of above 95%.

Effect of melatonin on cryopreservation of Beijing you chicken (gallus gallus) spermatozoa.

Beijing You Chicken, a valuable local chicken breed from Beijing, China, was once listed as an endangered breed. From the point of view of conservation, the preservation of this breed is an important task for the local researchers. Semen cryopreservation is a popular method to maintain valuable species. However, during cryopreservation, semen is susceptible to oxidative damage. Melatonin is a potent antioxidant and free radical scavenger, so it has been selected to improve the efficiency of sperm cryopreservation. In this study, the chicken semen was treated with different concentrations of melatonin in the cryopreservation solution. The results showed that melatonin at concentrations of 10-3 M and 10-5 M significantly improved sperm progressive motility and total motility, respectively, compared to the control (P < 0.05). Melatonin at 10-3 M also significantly improved the plasma membrane and acrosome integrity of spermatozoa compared to the control. The mechanisms are that melatonin significantly reduces the level of ROS and preserves sperm mitochondrial membrane potential. Most importantly, the melatonin-treated cryopreserved chicken sperm after artificial insemination significantly increased the hatching rate of chicks compared to the control (p < 0.05). The results show that melatonin has a positive effect on the quality of the cryopreserved spermatozoa. These results provide the theoretical and practical basis for using melatonin to improve Beijing You Chicken conservation, and they may also be applicable to poultry as a whole.

Maternal Exposure to Arsenic and Its Impact on Maternal and Fetal Health: A Review.

Arsenic exposure is a significant public health issue, with harmful effects caused by its use in commercial products such as car batteries, pesticides, and herbicides. Arsenic has three main compounds: inorganic, organic, and arsine gas. Inorganic arsenic compounds in water are highly toxic. The daily intake of arsenic from food and beverages is between 20 and 300 mcg/day. Arsenic is known for its carcinogenic properties and is classified as a human carcinogen by different institutions. Exposure can lead to oxidative stress, DNA damage, and epigenetic deregulation, which can cause endocrine disorders, altered signal transduction pathways, and cell proliferation. In addition, arsenic can easily cross the placenta, making it a critical concern for maternal and fetal health. Exposure can lead to complications such as gestational diabetes, anemia, low birth weight, miscarriage, and congenital anomalies. Female babies are particularly vulnerable to the negative impact of arsenic exposure, with a higher risk of low weight for gestational age and congenital cardiac anomalies. Therefore, it is crucial to monitor and regulate the levels of arsenic in drinking water and food sources to prevent these adverse health outcomes. Further research is necessary to fully understand the impact of arsenic exposure on human health, especially during pregnancy and infancy, by implementing preventative measures and monitoring the levels of arsenic in the environment.

The Effect of Microballoon Volume Fraction on the Elastic and Viscoelastic Properties of Hollow Microballoon-Filled Epoxy Composites.

This paper reports the study of hollow microballoon-filled epoxy composites also known as syntactic foams with various volume fractions of microballoons. Different mechanical and thermomechanical investigations were carried out to study the elastic and viscoelastic behavior of these foams. The density, void content, and microstructure of these materials were also studied for better characterization. In addition to the experimental testing, a representative 3D model of these syntactic foams was developed to further investigate their elastic behavior. The results indicate that changes in the volume percentage of the microballoons had a substantial impact on the elastic and viscoelastic behavior of these foams. These results will help in designing and optimizing custom-tailored syntactic foams for different engineering applications.

Prognostic gene HLA-DMA associated with cell cycle and immune infiltrates in LUAD.

BACKGROUND: The dominant subclass of non-small-cell lung cancer (NSCLC) is lung adenocarcinoma (LUAD). The tumor microenvironment (TME) is a crucial feature of carcinogenesis and progression in LUAD. Furthermore, immune and stromal components of TME are crucial factors to investigating and curing LUAD. Thus, the study assessed the value of TME-related genes for LUAD prognosis and immune infiltration. METHODS: All data were downloaded from TCGA and GEO databases. The immune and stromal scores were downloaded from ESTIMATE, and the association between the scores and prognosis was explored by Kaplan-Meier survival analysis. Protein-protein interaction (PPI) network and univariate Cox regression were used to find TME-related differentially expressed genes (DEGs), and HLA-DMA was regarded as a prognostic hub gene. Western blot analyses, qRT-PCR, and immunofluorescence were applied to verify HLA-DMA expression in clinical samples. NSCLC cell lines were used to verify the effect of HLA-DMA on cell proliferation and cell cycle distribution. At last, the alteration of immunotherapy response and TME transition caused by HLA-DMA different expression were further studied. RESULTS: The immune score was positively correlated with survival. The functional analyses suggested that TME-related DEGs may be involved in the immune response. The expression level of HLA-DMA was decreased in LUAD. In addition, HLA-DMA expression was associated with several clinical features and was positively associated with survival. Furthermore, HLA-DMA may suspend cell proliferation by regulating cell cycle. HLA-DMA expression was closely associated with immune infiltration and positively correlated with TMB, indicating that patients with high HLA-DMA level were more suitable for immunotherapy. CONCLUSION: These results reveal that HLA-DMA might act as a biomarker for immune infiltration and immunotherapy response.

Thermal and Mechanical Properties of Biocomposites Based on Polylactide and Tall Wheatgrass.

Biocomposites based on polylactic acid (PLA), tall wheatgrass (TWG), and hemp (H) were made by injection molding. The article discusses the impact of the agrofiller content on the composite properties, including thermal (DSC, DMA, and TG) and mechanical characteristics (tensile modulus, tensile strength, and impact strength). Generally, the introduction of a plant filler into the polylactide matrix reduced the thermal resistance of the resulting composites. Plant fillers influenced primarily the cold crystallization process, probably due to their nucleating properties. The addition of fillers to the PLA matrix resulted in an increased storage modulus across all tested temperatures compared to pure PLA. In the case of a composite with 50% of plant fillers, it was almost 118%. The mechanical properties of the tested composites depended significantly on the amount of plant filler used. It was observed that adding 50% of plant filler to PLA led to a twofold increase in tensile modulus and a decrease in tensile strength and impact strength by an average of 23 and 70%, respectively. It was determined that composites incorporating tall wheatgrass (TWG) particles exhibited a slightly elevated tensile modulus while showcasing a marginally reduced strength and impact resistance in comparison to composites containing hemp (H) components.