RESUMO
Extraction was optimized of polysaccharides from Gleoestereum incarnatum (GIP). The three parameters, extraction temperature, extraction time and the ratio of water to raw material, were optimized using the Box-Behnken design. As a result, the optimal extraction conditions were: extraction temperature 87.5 °C, extraction time 1 h and the ratio of water to raw material of 39.7 mL/g, where the highest yield of polysaccharide (13.18%) was obtained. GIP-II was the main fraction purified form GIP. GIP-II was composed of galactose, glucose, xylose, and mannose, with glucose was the predominant monosaccharide. GIP-II exhibited strong scavenging activities against DPPH and hydroxyl radials in vitro, as well as a strong inhibitory effect on the growth of HepG2 cells. The overall findings indicated that GIP-II is worthy of further exploration for its potential applications in antitumor drugs or health foods.