1.
Methods Mol Biol
; 1726: 29-34, 2018.
Artigo
em Inglês
| MEDLINE
| ID: mdl-29468541
RESUMO
It has increasingly been considered crucial the understanding of DNA methylation of Tumor Suppressor Gene (TSG) promoters, such as that of retinoblastoma 1 gene (RB1), and its role during carcinogenesis. We present a detailed and optimized protocol of the methylation-specific PCR (MSP) technique to study RB1 gene promoter hypermethylation.